BLASTX nr result
ID: Ephedra29_contig00009857
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Ephedra29_contig00009857
(547 letters)
Database: ./nr
115,041,592 sequences; 42,171,959,267 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
JAT67159.1 Protein FAM214B [Anthurium amnicola] 67 5e-14
KQJ90955.1 hypothetical protein BRADI_4g34840 [Brachypodium dist... 62 8e-14
ERN06234.1 hypothetical protein AMTR_s00016p00184960 [Amborella ... 65 1e-13
XP_011623540.1 PREDICTED: uncharacterized protein LOC18434426 [A... 65 1e-13
XP_003578404.1 PREDICTED: uncharacterized protein LOC100820958 i... 62 1e-13
KQJ90956.1 hypothetical protein BRADI_4g34840 [Brachypodium dist... 62 1e-13
EMT06546.1 hypothetical protein F775_16796 [Aegilops tauschii] 62 1e-13
XP_020159240.1 uncharacterized protein LOC109744522 [Aegilops ta... 62 1e-13
XP_010238343.1 PREDICTED: uncharacterized protein LOC100820958 i... 61 2e-13
XP_019701643.1 PREDICTED: uncharacterized protein LOC105061402 i... 69 2e-13
XP_010943732.2 PREDICTED: uncharacterized protein LOC105061402 i... 69 2e-13
XP_019701644.1 PREDICTED: uncharacterized protein LOC105061402 i... 69 2e-13
XP_010943735.1 PREDICTED: uncharacterized protein LOC105061402 i... 69 2e-13
XP_010943737.1 PREDICTED: uncharacterized protein LOC105061402 i... 69 2e-13
XP_010943736.1 PREDICTED: uncharacterized protein LOC105061402 i... 69 2e-13
JAT49805.1 Protein FAM214B [Anthurium amnicola] 67 2e-13
EMS50633.1 hypothetical protein TRIUR3_09702 [Triticum urartu] 61 4e-13
BAK07297.1 predicted protein [Hordeum vulgare subsp. vulgare] BA... 60 5e-13
ONK72059.1 uncharacterized protein A4U43_C04F15260 [Asparagus of... 62 5e-13
BAK02814.1 predicted protein, partial [Hordeum vulgare subsp. vu... 60 5e-13
>JAT67159.1 Protein FAM214B [Anthurium amnicola]
Length = 782
Score = 67.0 bits (162), Expect(2) = 5e-14
Identities = 30/50 (60%), Positives = 40/50 (80%)
Frame = -1
Query: 280 EKIEGFLTVLNVTRGSFSTPS*KIPFSVNNVDADKTCLYYALVDIAGGMP 131
++++GFL VLNVT G+FS PS K+PFSV +VD D + LYYA +D+AG MP
Sbjct: 423 KRLDGFLAVLNVTGGNFSPPSRKLPFSVISVDGDSSLLYYASIDLAGNMP 472
Score = 37.7 bits (86), Expect(2) = 5e-14
Identities = 19/32 (59%), Positives = 24/32 (75%)
Frame = -2
Query: 114 VAERKKSSKNEDSRASKSRF*IPVRGRIQLVL 19
V + ++S NEDSRASK R IP++G IQLVL
Sbjct: 478 VPKMRRSLSNEDSRASKCRLRIPMKGCIQLVL 509
>KQJ90955.1 hypothetical protein BRADI_4g34840 [Brachypodium distachyon]
Length = 502
Score = 61.6 bits (148), Expect(2) = 8e-14
Identities = 26/50 (52%), Positives = 38/50 (76%)
Frame = -1
Query: 280 EKIEGFLTVLNVTRGSFSTPS*KIPFSVNNVDADKTCLYYALVDIAGGMP 131
+ I GFL VLNVT G+FS P+ K+PF+V ++D D + LYY+ +D+AG +P
Sbjct: 411 QNINGFLAVLNVTGGTFSPPTQKLPFAVTSIDEDSSLLYYSSIDLAGRLP 460
Score = 42.4 bits (98), Expect(2) = 8e-14
Identities = 20/35 (57%), Positives = 26/35 (74%)
Frame = -2
Query: 123 NPNVAERKKSSKNEDSRASKSRF*IPVRGRIQLVL 19
N + K+SS N DSR++KSR IPV+GRIQLV+
Sbjct: 463 NSKSPKHKRSSNNNDSRSAKSRLHIPVKGRIQLVM 497
>ERN06234.1 hypothetical protein AMTR_s00016p00184960 [Amborella trichopoda]
Length = 805
Score = 65.1 bits (157), Expect(2) = 1e-13
Identities = 29/49 (59%), Positives = 38/49 (77%)
Frame = -1
Query: 280 EKIEGFLTVLNVTRGSFSTPS*KIPFSVNNVDADKTCLYYALVDIAGGM 134
++I+GFL +LNVT GSFS PS K+PFSV +VD D LYYA +D+AG +
Sbjct: 404 QRIDGFLAILNVTGGSFSPPSQKLPFSVTSVDGDSYLLYYASIDLAGNL 452
Score = 38.5 bits (88), Expect(2) = 1e-13
Identities = 18/28 (64%), Positives = 23/28 (82%)
Frame = -2
Query: 102 KKSSKNEDSRASKSRF*IPVRGRIQLVL 19
K+S NEDSR++KS IP++GRIQLVL
Sbjct: 463 KRSLSNEDSRSNKSHLRIPMKGRIQLVL 490
>XP_011623540.1 PREDICTED: uncharacterized protein LOC18434426 [Amborella
trichopoda]
Length = 759
Score = 65.1 bits (157), Expect(2) = 1e-13
Identities = 29/49 (59%), Positives = 38/49 (77%)
Frame = -1
Query: 280 EKIEGFLTVLNVTRGSFSTPS*KIPFSVNNVDADKTCLYYALVDIAGGM 134
++I+GFL +LNVT GSFS PS K+PFSV +VD D LYYA +D+AG +
Sbjct: 404 QRIDGFLAILNVTGGSFSPPSQKLPFSVTSVDGDSYLLYYASIDLAGNL 452
Score = 38.5 bits (88), Expect(2) = 1e-13
Identities = 18/28 (64%), Positives = 23/28 (82%)
Frame = -2
Query: 102 KKSSKNEDSRASKSRF*IPVRGRIQLVL 19
K+S NEDSR++KS IP++GRIQLVL
Sbjct: 463 KRSLSNEDSRSNKSHLRIPMKGRIQLVL 490
>XP_003578404.1 PREDICTED: uncharacterized protein LOC100820958 isoform X1
[Brachypodium distachyon] XP_010238341.1 PREDICTED:
uncharacterized protein LOC100820958 isoform X1
[Brachypodium distachyon] XP_014758356.1 PREDICTED:
uncharacterized protein LOC100820958 isoform X1
[Brachypodium distachyon] KQJ90957.1 hypothetical
protein BRADI_4g34840 [Brachypodium distachyon]
Length = 734
Score = 61.6 bits (148), Expect(2) = 1e-13
Identities = 26/50 (52%), Positives = 38/50 (76%)
Frame = -1
Query: 280 EKIEGFLTVLNVTRGSFSTPS*KIPFSVNNVDADKTCLYYALVDIAGGMP 131
+ I GFL VLNVT G+FS P+ K+PF+V ++D D + LYY+ +D+AG +P
Sbjct: 411 QNINGFLAVLNVTGGTFSPPTQKLPFAVTSIDEDSSLLYYSSIDLAGRLP 460
Score = 42.0 bits (97), Expect(2) = 1e-13
Identities = 20/35 (57%), Positives = 26/35 (74%)
Frame = -2
Query: 123 NPNVAERKKSSKNEDSRASKSRF*IPVRGRIQLVL 19
N + K+SS N DSR++KSR IPV+GRIQLV+
Sbjct: 463 NSKSPKHKRSSNNNDSRSAKSRLHIPVKGRIQLVV 497
>KQJ90956.1 hypothetical protein BRADI_4g34840 [Brachypodium distachyon]
Length = 577
Score = 61.6 bits (148), Expect(2) = 1e-13
Identities = 26/50 (52%), Positives = 38/50 (76%)
Frame = -1
Query: 280 EKIEGFLTVLNVTRGSFSTPS*KIPFSVNNVDADKTCLYYALVDIAGGMP 131
+ I GFL VLNVT G+FS P+ K+PF+V ++D D + LYY+ +D+AG +P
Sbjct: 411 QNINGFLAVLNVTGGTFSPPTQKLPFAVTSIDEDSSLLYYSSIDLAGRLP 460
Score = 42.0 bits (97), Expect(2) = 1e-13
Identities = 20/35 (57%), Positives = 26/35 (74%)
Frame = -2
Query: 123 NPNVAERKKSSKNEDSRASKSRF*IPVRGRIQLVL 19
N + K+SS N DSR++KSR IPV+GRIQLV+
Sbjct: 463 NSKSPKHKRSSNNNDSRSAKSRLHIPVKGRIQLVV 497
>EMT06546.1 hypothetical protein F775_16796 [Aegilops tauschii]
Length = 787
Score = 62.4 bits (150), Expect(2) = 1e-13
Identities = 26/50 (52%), Positives = 39/50 (78%)
Frame = -1
Query: 280 EKIEGFLTVLNVTRGSFSTPS*KIPFSVNNVDADKTCLYYALVDIAGGMP 131
+ I+GFL VLNVT G+FS P+ K+PF+V ++D D + LYY+ +D+AG +P
Sbjct: 468 QNIDGFLAVLNVTGGNFSPPTQKLPFAVTSIDEDSSLLYYSSIDLAGRLP 517
Score = 40.8 bits (94), Expect(2) = 1e-13
Identities = 19/35 (54%), Positives = 26/35 (74%)
Frame = -2
Query: 123 NPNVAERKKSSKNEDSRASKSRF*IPVRGRIQLVL 19
N + K+SS N DSR+S+SR IP++GRIQLV+
Sbjct: 520 NSKSPKLKRSSNNNDSRSSRSRLHIPIKGRIQLVV 554
>XP_020159240.1 uncharacterized protein LOC109744522 [Aegilops tauschii subsp.
tauschii] XP_020159241.1 uncharacterized protein
LOC109744522 [Aegilops tauschii subsp. tauschii]
XP_020159243.1 uncharacterized protein LOC109744522
[Aegilops tauschii subsp. tauschii] XP_020159244.1
uncharacterized protein LOC109744522 [Aegilops tauschii
subsp. tauschii] XP_020168436.1 uncharacterized protein
LOC109753927 [Aegilops tauschii subsp. tauschii]
XP_020168437.1 uncharacterized protein LOC109753927
[Aegilops tauschii subsp. tauschii] XP_020168438.1
uncharacterized protein LOC109753927 [Aegilops tauschii
subsp. tauschii] XP_020168439.1 uncharacterized protein
LOC109753927 [Aegilops tauschii subsp. tauschii]
Length = 732
Score = 62.4 bits (150), Expect(2) = 1e-13
Identities = 26/50 (52%), Positives = 39/50 (78%)
Frame = -1
Query: 280 EKIEGFLTVLNVTRGSFSTPS*KIPFSVNNVDADKTCLYYALVDIAGGMP 131
+ I+GFL VLNVT G+FS P+ K+PF+V ++D D + LYY+ +D+AG +P
Sbjct: 413 QNIDGFLAVLNVTGGNFSPPTQKLPFAVTSIDEDSSLLYYSSIDLAGRLP 462
Score = 40.8 bits (94), Expect(2) = 1e-13
Identities = 19/35 (54%), Positives = 26/35 (74%)
Frame = -2
Query: 123 NPNVAERKKSSKNEDSRASKSRF*IPVRGRIQLVL 19
N + K+SS N DSR+S+SR IP++GRIQLV+
Sbjct: 465 NSKSPKLKRSSNNNDSRSSRSRLHIPIKGRIQLVV 499
>XP_010238343.1 PREDICTED: uncharacterized protein LOC100820958 isoform X2
[Brachypodium distachyon]
Length = 362
Score = 60.8 bits (146), Expect(2) = 2e-13
Identities = 26/48 (54%), Positives = 37/48 (77%)
Frame = -1
Query: 274 IEGFLTVLNVTRGSFSTPS*KIPFSVNNVDADKTCLYYALVDIAGGMP 131
I GFL VLNVT G+FS P+ K+PF+V ++D D + LYY+ +D+AG +P
Sbjct: 41 INGFLAVLNVTGGTFSPPTQKLPFAVTSIDEDSSLLYYSSIDLAGRLP 88
Score = 42.0 bits (97), Expect(2) = 2e-13
Identities = 20/35 (57%), Positives = 26/35 (74%)
Frame = -2
Query: 123 NPNVAERKKSSKNEDSRASKSRF*IPVRGRIQLVL 19
N + K+SS N DSR++KSR IPV+GRIQLV+
Sbjct: 91 NSKSPKHKRSSNNNDSRSAKSRLHIPVKGRIQLVV 125
>XP_019701643.1 PREDICTED: uncharacterized protein LOC105061402 isoform X1 [Elaeis
guineensis]
Length = 804
Score = 68.9 bits (167), Expect(2) = 2e-13
Identities = 29/51 (56%), Positives = 42/51 (82%)
Frame = -1
Query: 283 TEKIEGFLTVLNVTRGSFSTPS*KIPFSVNNVDADKTCLYYALVDIAGGMP 131
+++I+GFL VLNVTRG+FS PS K+PF+V ++D D + LYYA +D+AG +P
Sbjct: 426 SQRIDGFLAVLNVTRGNFSPPSQKLPFAVTSIDGDSSLLYYASIDLAGRLP 476
Score = 33.5 bits (75), Expect(2) = 2e-13
Identities = 17/28 (60%), Positives = 21/28 (75%)
Frame = -2
Query: 102 KKSSKNEDSRASKSRF*IPVRGRIQLVL 19
++S N DSRA KSR IP++G IQLVL
Sbjct: 486 RRSLSNGDSRAVKSRLRIPMKGCIQLVL 513
>XP_010943732.2 PREDICTED: uncharacterized protein LOC105061402 isoform X2 [Elaeis
guineensis]
Length = 803
Score = 68.9 bits (167), Expect(2) = 2e-13
Identities = 29/51 (56%), Positives = 42/51 (82%)
Frame = -1
Query: 283 TEKIEGFLTVLNVTRGSFSTPS*KIPFSVNNVDADKTCLYYALVDIAGGMP 131
+++I+GFL VLNVTRG+FS PS K+PF+V ++D D + LYYA +D+AG +P
Sbjct: 426 SQRIDGFLAVLNVTRGNFSPPSQKLPFAVTSIDGDSSLLYYASIDLAGRLP 476
Score = 33.5 bits (75), Expect(2) = 2e-13
Identities = 17/28 (60%), Positives = 21/28 (75%)
Frame = -2
Query: 102 KKSSKNEDSRASKSRF*IPVRGRIQLVL 19
++S N DSRA KSR IP++G IQLVL
Sbjct: 486 RRSLSNGDSRAVKSRLRIPMKGCIQLVL 513
>XP_019701644.1 PREDICTED: uncharacterized protein LOC105061402 isoform X3 [Elaeis
guineensis]
Length = 802
Score = 68.9 bits (167), Expect(2) = 2e-13
Identities = 29/51 (56%), Positives = 42/51 (82%)
Frame = -1
Query: 283 TEKIEGFLTVLNVTRGSFSTPS*KIPFSVNNVDADKTCLYYALVDIAGGMP 131
+++I+GFL VLNVTRG+FS PS K+PF+V ++D D + LYYA +D+AG +P
Sbjct: 426 SQRIDGFLAVLNVTRGNFSPPSQKLPFAVTSIDGDSSLLYYASIDLAGRLP 476
Score = 33.5 bits (75), Expect(2) = 2e-13
Identities = 17/28 (60%), Positives = 21/28 (75%)
Frame = -2
Query: 102 KKSSKNEDSRASKSRF*IPVRGRIQLVL 19
++S N DSRA KSR IP++G IQLVL
Sbjct: 486 RRSLSNGDSRAVKSRLRIPMKGCIQLVL 513
>XP_010943735.1 PREDICTED: uncharacterized protein LOC105061402 isoform X6 [Elaeis
guineensis]
Length = 767
Score = 68.9 bits (167), Expect(2) = 2e-13
Identities = 29/51 (56%), Positives = 42/51 (82%)
Frame = -1
Query: 283 TEKIEGFLTVLNVTRGSFSTPS*KIPFSVNNVDADKTCLYYALVDIAGGMP 131
+++I+GFL VLNVTRG+FS PS K+PF+V ++D D + LYYA +D+AG +P
Sbjct: 426 SQRIDGFLAVLNVTRGNFSPPSQKLPFAVTSIDGDSSLLYYASIDLAGRLP 476
Score = 33.5 bits (75), Expect(2) = 2e-13
Identities = 17/28 (60%), Positives = 21/28 (75%)
Frame = -2
Query: 102 KKSSKNEDSRASKSRF*IPVRGRIQLVL 19
++S N DSRA KSR IP++G IQLVL
Sbjct: 486 RRSLSNGDSRAVKSRLRIPMKGCIQLVL 513
>XP_010943737.1 PREDICTED: uncharacterized protein LOC105061402 isoform X8 [Elaeis
guineensis]
Length = 766
Score = 68.9 bits (167), Expect(2) = 2e-13
Identities = 29/51 (56%), Positives = 42/51 (82%)
Frame = -1
Query: 283 TEKIEGFLTVLNVTRGSFSTPS*KIPFSVNNVDADKTCLYYALVDIAGGMP 131
+++I+GFL VLNVTRG+FS PS K+PF+V ++D D + LYYA +D+AG +P
Sbjct: 426 SQRIDGFLAVLNVTRGNFSPPSQKLPFAVTSIDGDSSLLYYASIDLAGRLP 476
Score = 33.5 bits (75), Expect(2) = 2e-13
Identities = 17/28 (60%), Positives = 21/28 (75%)
Frame = -2
Query: 102 KKSSKNEDSRASKSRF*IPVRGRIQLVL 19
++S N DSRA KSR IP++G IQLVL
Sbjct: 486 RRSLSNGDSRAVKSRLRIPMKGCIQLVL 513
>XP_010943736.1 PREDICTED: uncharacterized protein LOC105061402 isoform X7 [Elaeis
guineensis]
Length = 765
Score = 68.9 bits (167), Expect(2) = 2e-13
Identities = 29/51 (56%), Positives = 42/51 (82%)
Frame = -1
Query: 283 TEKIEGFLTVLNVTRGSFSTPS*KIPFSVNNVDADKTCLYYALVDIAGGMP 131
+++I+GFL VLNVTRG+FS PS K+PF+V ++D D + LYYA +D+AG +P
Sbjct: 426 SQRIDGFLAVLNVTRGNFSPPSQKLPFAVTSIDGDSSLLYYASIDLAGRLP 476
Score = 33.5 bits (75), Expect(2) = 2e-13
Identities = 17/28 (60%), Positives = 21/28 (75%)
Frame = -2
Query: 102 KKSSKNEDSRASKSRF*IPVRGRIQLVL 19
++S N DSRA KSR IP++G IQLVL
Sbjct: 486 RRSLSNGDSRAVKSRLRIPMKGCIQLVL 513
>JAT49805.1 Protein FAM214B [Anthurium amnicola]
Length = 515
Score = 67.0 bits (162), Expect(2) = 2e-13
Identities = 30/50 (60%), Positives = 40/50 (80%)
Frame = -1
Query: 280 EKIEGFLTVLNVTRGSFSTPS*KIPFSVNNVDADKTCLYYALVDIAGGMP 131
++++GFL VLNVT G+FS PS K+PFSV +VD D + LYYA +D+AG MP
Sbjct: 423 KRLDGFLAVLNVTGGNFSPPSRKLPFSVISVDGDSSLLYYASIDLAGNMP 472
Score = 35.4 bits (80), Expect(2) = 2e-13
Identities = 18/32 (56%), Positives = 23/32 (71%)
Frame = -2
Query: 114 VAERKKSSKNEDSRASKSRF*IPVRGRIQLVL 19
V + ++S NEDSRASK R IP++G IQL L
Sbjct: 478 VPKMRRSLSNEDSRASKCRLRIPMKGCIQLEL 509
>EMS50633.1 hypothetical protein TRIUR3_09702 [Triticum urartu]
Length = 707
Score = 60.8 bits (146), Expect(2) = 4e-13
Identities = 25/48 (52%), Positives = 38/48 (79%)
Frame = -1
Query: 274 IEGFLTVLNVTRGSFSTPS*KIPFSVNNVDADKTCLYYALVDIAGGMP 131
I+GFL VLNVT G+FS P+ K+PF+V ++D D + +YY+ +D+AG +P
Sbjct: 389 IDGFLAVLNVTGGNFSPPTQKLPFAVTSIDEDSSLMYYSSIDLAGRLP 436
Score = 40.8 bits (94), Expect(2) = 4e-13
Identities = 19/35 (54%), Positives = 26/35 (74%)
Frame = -2
Query: 123 NPNVAERKKSSKNEDSRASKSRF*IPVRGRIQLVL 19
N + K+SS N DSR+S+SR IP++GRIQLV+
Sbjct: 439 NSKSPKLKRSSNNNDSRSSRSRLHIPIKGRIQLVV 473
>BAK07297.1 predicted protein [Hordeum vulgare subsp. vulgare] BAJ93136.1
predicted protein [Hordeum vulgare subsp. vulgare]
Length = 732
Score = 60.5 bits (145), Expect(2) = 5e-13
Identities = 25/50 (50%), Positives = 38/50 (76%)
Frame = -1
Query: 280 EKIEGFLTVLNVTRGSFSTPS*KIPFSVNNVDADKTCLYYALVDIAGGMP 131
+ I+GFL VLNVT G+F P+ K+PF+V ++D D + LYY+ +D+AG +P
Sbjct: 413 QNIDGFLAVLNVTGGNFCPPTQKLPFAVTSIDEDSSLLYYSSIDLAGRLP 462
Score = 40.8 bits (94), Expect(2) = 5e-13
Identities = 19/35 (54%), Positives = 26/35 (74%)
Frame = -2
Query: 123 NPNVAERKKSSKNEDSRASKSRF*IPVRGRIQLVL 19
N + K+SS N DSR+S+SR IP++GRIQLV+
Sbjct: 465 NSKSPKLKRSSNNNDSRSSRSRLHIPIKGRIQLVV 499
>ONK72059.1 uncharacterized protein A4U43_C04F15260 [Asparagus officinalis]
Length = 714
Score = 61.6 bits (148), Expect(2) = 5e-13
Identities = 26/50 (52%), Positives = 38/50 (76%)
Frame = -1
Query: 283 TEKIEGFLTVLNVTRGSFSTPS*KIPFSVNNVDADKTCLYYALVDIAGGM 134
+++I+GFL VLN+T GSFS S K+PF V ++D D + LYYA +D+AG +
Sbjct: 391 SQRIDGFLAVLNITGGSFSPASQKLPFGVTSIDGDSSLLYYASIDLAGSL 440
Score = 39.7 bits (91), Expect(2) = 5e-13
Identities = 19/28 (67%), Positives = 23/28 (82%)
Frame = -2
Query: 102 KKSSKNEDSRASKSRF*IPVRGRIQLVL 19
K+S N DSRA+KSR IP++GRIQLVL
Sbjct: 451 KRSLSNNDSRAAKSRLRIPMKGRIQLVL 478
>BAK02814.1 predicted protein, partial [Hordeum vulgare subsp. vulgare]
Length = 710
Score = 60.5 bits (145), Expect(2) = 5e-13
Identities = 25/50 (50%), Positives = 38/50 (76%)
Frame = -1
Query: 280 EKIEGFLTVLNVTRGSFSTPS*KIPFSVNNVDADKTCLYYALVDIAGGMP 131
+ I+GFL VLNVT G+F P+ K+PF+V ++D D + LYY+ +D+AG +P
Sbjct: 391 QNIDGFLAVLNVTGGNFCPPTQKLPFAVTSIDEDSSLLYYSSIDLAGRLP 440
Score = 40.8 bits (94), Expect(2) = 5e-13
Identities = 19/35 (54%), Positives = 26/35 (74%)
Frame = -2
Query: 123 NPNVAERKKSSKNEDSRASKSRF*IPVRGRIQLVL 19
N + K+SS N DSR+S+SR IP++GRIQLV+
Sbjct: 443 NSKSPKLKRSSNNNDSRSSRSRLHIPIKGRIQLVV 477