BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Ephedra29_contig00005253
         (2248 letters)

Database: ./nr 
           115,041,592 sequences; 42,171,959,267 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AGX45528.1 4-coumarate:CoA ligase [Pinus taeda]                       770   0.0  
AFX98059.1 4-coumarate:CoA ligase [Cunninghamia lanceolata]           745   0.0  
AEX32786.1 coumaroyl-CoA ligase [Vitis vinifera]                      736   0.0  
XP_002274994.1 PREDICTED: 4-coumarate--CoA ligase 2 [Vitis vinif...   733   0.0  
XP_002520028.1 PREDICTED: 4-coumarate--CoA ligase 2 [Ricinus com...   726   0.0  
XP_018817126.1 PREDICTED: 4-coumarate--CoA ligase 2-like [Juglan...   724   0.0  
AIS92506.1 4-coumarate:CoA ligase 2 [Epimedium sagittatum]            723   0.0  
XP_019199737.1 PREDICTED: 4-coumarate--CoA ligase 2 [Ipomoea nil]     723   0.0  
XP_010034948.2 PREDICTED: LOW QUALITY PROTEIN: 4-coumarate--CoA ...   723   0.0  
XP_010261545.1 PREDICTED: 4-coumarate--CoA ligase 2 isoform X1 [...   722   0.0  
AFG26323.1 4-coumarate-CoA ligase [Cinnamomum osmophloeum]            720   0.0  
AMN10098.1 4-coumarate-CoA ligase [Ginkgo biloba]                     719   0.0  
XP_008234565.1 PREDICTED: 4-coumarate--CoA ligase 2 [Prunus mume]     721   0.0  
AKG06666.1 4-coumarate-CoA ligase 2 [Ziziphus jujuba]                 718   0.0  
XP_016202253.1 PREDICTED: 4-coumarate--CoA ligase 2 [Arachis ipa...   717   0.0  
XP_015964696.1 PREDICTED: 4-coumarate--CoA ligase 2 [Arachis dur...   717   0.0  
ALM23512.1 4-coumarate-CoA ligase 2 [Ziziphus jujuba]                 717   0.0  
OAY41753.1 hypothetical protein MANES_09G127000 [Manihot esculenta]   716   0.0  
BAG82851.1 4-coumarate:CoA ligase [Ipomoea batatas]                   716   0.0  
ADZ54779.1 4-coumarate-CoA ligase [Prunus avium]                      717   0.0  

>AGX45528.1 4-coumarate:CoA ligase [Pinus taeda]
          Length = 575

 Score =  770 bits (1988), Expect = 0.0
 Identities = 397/578 (68%), Positives = 462/578 (79%), Gaps = 3/578 (0%)
 Frame = +3

Query: 174  MIEVKCMATVPQITEPESPSVKVLEKKTEEIHKMEEYVFRSRLPDIYIPDNMSLHDYCFE 353
            MIEV+    + + TE E+      E        M+ +++RS+LPDI IP ++ LH YCFE
Sbjct: 1    MIEVQSAPPMARSTENENNQHDAEEGAVLNEGGMD-FLYRSKLPDIDIPYHLPLHSYCFE 59

Query: 354  RLHELRDRPCLIQGSTGKILTYGEVEASSRRVAAGLRKIGMAKGEVVMLLLQNCPEFVIT 533
            +L ELR++PCLIQGS GKI +YGEVE  SR+VA+GL K+G  KG+VVMLLL NCPEFV  
Sbjct: 60   KLDELREKPCLIQGSNGKIYSYGEVELISRKVASGLAKLGFKKGDVVMLLLPNCPEFVFV 119

Query: 534  FLGASMGGNVVTTANPFYTPADIAKQASASNTRIVVTSSSYVNKLGDLMSG-GVRVFTVD 710
            FLGASM G + TTANPFYTP+DIAKQ  AS  R++VT ++ V KL DLM   GV+V T+D
Sbjct: 120  FLGASMAGAIATTANPFYTPSDIAKQRGASGARLIVTHAACVKKLRDLMENHGVQVITID 179

Query: 711  EPQEGCEHFSVLTDVADEGPL--GNVSVDAEDTVALPYSSGTTGLPKGVMLTHRSLVTSV 884
             P +GCEH S+L D  DE      +  V  +DTVALPYSSGTTGLPKGVMLTH+ LV+SV
Sbjct: 180  NPPKGCEHISLLLD-GDENEYCPADCIVQPDDTVALPYSSGTTGLPKGVMLTHKGLVSSV 238

Query: 885  AQQVDGDNPNLNLLPEDVVLCVLPLFHIYSLNSVLLCSLRAGSAVLLMHKFEIGNLLEFV 1064
            AQ VDG+NPNL L  EDVVLCVLPLFHIYSLNSVLLCSLRAGSA+LLMHKFEIG+LL+ V
Sbjct: 239  AQLVDGENPNLYLHSEDVVLCVLPLFHIYSLNSVLLCSLRAGSAILLMHKFEIGSLLDLV 298

Query: 1065 PKYKISXXXXXXXXXXXXXKNQMVESYDLSSVRFVLSGAAPLGKELEEALRKRVPNAIFG 1244
             ++K++             KN +VESYDLSS+R VLSGAAPLGKELE+ALR R+P A FG
Sbjct: 299  QRFKVTVAPVVPPIVLAFAKNALVESYDLSSIRVVLSGAAPLGKELEDALRLRLPKATFG 358

Query: 1245 QGYGMTEAGPVLSMCLGFAKEPTPMKFGSCGCVVRNAEMKIVDSDTGISLPRNKPGEICI 1424
            QGYGMTEAGPVLSMCL FAKEP PMK GSCG VVRNA+MKI+D DTG  LP N+PGEICI
Sbjct: 359  QGYGMTEAGPVLSMCLAFAKEPFPMKSGSCGTVVRNAQMKIIDPDTGTCLPYNQPGEICI 418

Query: 1425 RGAQIMKGYLNDPDSTARTIDEEGWLHTGDIGYVDDDDEVFIIDRVKEIIKYKGFQVPPA 1604
            RG QIMKGYLND +STARTIDE+GWLHTGDIGY+DDD+EVFI+DRVKEIIKYKGFQVPPA
Sbjct: 419  RGPQIMKGYLNDAESTARTIDEDGWLHTGDIGYIDDDEEVFIVDRVKEIIKYKGFQVPPA 478

Query: 1605 ELEALLINHPSIADAAVVPLNDELAGEVPVAFLVKSENGPGVTEEDIKQFVAKQVVYYKR 1784
            ELEA+LI HPSIADAAVVP  DE+AGEVPVAF+V+S NG  +TE++IKQFVAKQVV+YK+
Sbjct: 479  ELEAILITHPSIADAAVVPQKDEVAGEVPVAFVVRS-NGFDLTEDEIKQFVAKQVVFYKK 537

Query: 1785 LHKVNFIHAIPKSPSGKILRKDLKAKLNEPIPNEEPEA 1898
            LHKV FIHAIPKSPSGKILRKDL+AKL+ P    E +A
Sbjct: 538  LHKVYFIHAIPKSPSGKILRKDLRAKLSAPTSTVEIKA 575


>AFX98059.1 4-coumarate:CoA ligase [Cunninghamia lanceolata]
          Length = 565

 Score =  745 bits (1924), Expect = 0.0
 Identities = 373/571 (65%), Positives = 452/571 (79%)
 Frame = +3

Query: 174  MIEVKCMATVPQITEPESPSVKVLEKKTEEIHKMEEYVFRSRLPDIYIPDNMSLHDYCFE 353
            MI+V+   +VP +  P      ++      +   +EY++RS+LPDI IP ++ LH YCFE
Sbjct: 1    MIQVEDKPSVPLMPMPTPTPPSLINDNNSPV---KEYLYRSKLPDIDIPYHLPLHTYCFE 57

Query: 354  RLHELRDRPCLIQGSTGKILTYGEVEASSRRVAAGLRKIGMAKGEVVMLLLQNCPEFVIT 533
             + +++++PCLI+GS+GKI TYG+VE  SR+ A+GL K+G+ KG+VVMLLL NCPEF   
Sbjct: 58   NVEQVKEKPCLIEGSSGKIYTYGDVELISRKAASGLSKMGIGKGDVVMLLLHNCPEFAFV 117

Query: 534  FLGASMGGNVVTTANPFYTPADIAKQASASNTRIVVTSSSYVNKLGDLMSGGVRVFTVDE 713
            FLG S+ G VVTTANPFYTPADI+KQ  ASN RIVVT ++YV KL D +   ++V TVD 
Sbjct: 118  FLGTSISGGVVTTANPFYTPADISKQLRASNARIVVTHAAYVEKLTDFLPH-LKVLTVDN 176

Query: 714  PQEGCEHFSVLTDVADEGPLGNVSVDAEDTVALPYSSGTTGLPKGVMLTHRSLVTSVAQQ 893
            P EGC H S L    DE     V +  +D VALPYSSGTTGLPKGV+LTH+SL++SVAQQ
Sbjct: 177  PPEGCSHISELLQ-GDEEKCPAVEIHPDDAVALPYSSGTTGLPKGVILTHKSLLSSVAQQ 235

Query: 894  VDGDNPNLNLLPEDVVLCVLPLFHIYSLNSVLLCSLRAGSAVLLMHKFEIGNLLEFVPKY 1073
            VDG NPNL L  EDVVLCVLPLFHIYSLNSVLLCSLRAG+A+LLMHKFEI  LL  +  Y
Sbjct: 236  VDGLNPNLYLHSEDVVLCVLPLFHIYSLNSVLLCSLRAGAAILLMHKFEIATLLHLIHTY 295

Query: 1074 KISXXXXXXXXXXXXXKNQMVESYDLSSVRFVLSGAAPLGKELEEALRKRVPNAIFGQGY 1253
            K++             KN M++ +DL+S+R VLSGAAPLGKELE AL  R+P A+FGQGY
Sbjct: 296  KVTVAAVVPPIVLAIAKNPMIDHHDLTSIRIVLSGAAPLGKELELALTTRIPRAVFGQGY 355

Query: 1254 GMTEAGPVLSMCLGFAKEPTPMKFGSCGCVVRNAEMKIVDSDTGISLPRNKPGEICIRGA 1433
            GMTEAGPVLSMCLGFAKEP P K GSCG VVRNA+MKI+D +TG SLP NKPGEICIRG 
Sbjct: 356  GMTEAGPVLSMCLGFAKEPFPTKSGSCGTVVRNAQMKIIDPETGESLPYNKPGEICIRGP 415

Query: 1434 QIMKGYLNDPDSTARTIDEEGWLHTGDIGYVDDDDEVFIIDRVKEIIKYKGFQVPPAELE 1613
            QIMKGYLNDP++TARTID++GWLHTGDIGY+D+ +EVFI+DRVKE+IKYKGFQVPPAELE
Sbjct: 416  QIMKGYLNDPEATARTIDKDGWLHTGDIGYIDEGEEVFIVDRVKELIKYKGFQVPPAELE 475

Query: 1614 ALLINHPSIADAAVVPLNDELAGEVPVAFLVKSENGPGVTEEDIKQFVAKQVVYYKRLHK 1793
            ++LI HPSIADAAVVP  +E AGEVPVAF+V+S NG  +TE++IKQFVAKQVV+YKRLHK
Sbjct: 476  SILITHPSIADAAVVPQKNEAAGEVPVAFVVRS-NGFDLTEDEIKQFVAKQVVFYKRLHK 534

Query: 1794 VNFIHAIPKSPSGKILRKDLKAKLNEPIPNE 1886
            V F+HAIPKSP+GKILRKDL+AKL  P+ ++
Sbjct: 535  VYFVHAIPKSPAGKILRKDLRAKLEAPVEDK 565


>AEX32786.1 coumaroyl-CoA ligase [Vitis vinifera]
          Length = 570

 Score =  736 bits (1899), Expect = 0.0
 Identities = 369/565 (65%), Positives = 450/565 (79%), Gaps = 10/565 (1%)
 Frame = +3

Query: 216  EPESPSVKVLEKK---TEEIHKMEEYVFRSRLPDIYIPDNMSLHDYCFERLHELRDRPCL 386
            E ++P V  L+      +  ++++++VFRS+LP+I I +++ LH YCFE   +  DRPCL
Sbjct: 5    ETQNPDVSNLDTSHSIPKMANRIDDHVFRSKLPEIPISNHLPLHTYCFENYSQFADRPCL 64

Query: 387  IQGSTGKILTYGEVEASSRRVAAGLRKIGMAKGEVVMLLLQNCPEFVITFLGASMGGNVV 566
            I GST K  ++ E    SR+V AG   +G+ +G+VVM+LLQNC EF  +FLGASM G V 
Sbjct: 65   IVGSTNKTYSFAETHLISRKVGAGFAHLGLKQGDVVMILLQNCAEFAFSFLGASMVGAVT 124

Query: 567  TTANPFYTPADIAKQASASNTRIVVTSSSYVNKLGDLMSG-------GVRVFTVDEPQEG 725
            TTANPFYT A+I KQ +AS  +IVVT + YV+KL D   G       G  V T+D+P E 
Sbjct: 125  TTANPFYTSAEIFKQLNASKAKIVVTQAQYVDKLRDYPDGQVAKIGEGFTVITIDDPPEN 184

Query: 726  CEHFSVLTDVADEGPLGNVSVDAEDTVALPYSSGTTGLPKGVMLTHRSLVTSVAQQVDGD 905
            C HFSV+++ A+E  L  VS++++D VALP+SSGTTGLPKGV+LTH+SL+TSVAQQVDG+
Sbjct: 185  CMHFSVVSE-ANESELPEVSINSDDPVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGE 243

Query: 906  NPNLNLLPEDVVLCVLPLFHIYSLNSVLLCSLRAGSAVLLMHKFEIGNLLEFVPKYKISX 1085
            NPNL+L P+DVVLCVLPLFHIYSLNSVLLCSLRAG+AVLLM KFEIG LLE + +Y++S 
Sbjct: 244  NPNLHLTPDDVVLCVLPLFHIYSLNSVLLCSLRAGAAVLLMQKFEIGTLLELIQRYRVSV 303

Query: 1086 XXXXXXXXXXXXKNQMVESYDLSSVRFVLSGAAPLGKELEEALRKRVPNAIFGQGYGMTE 1265
                        KN MVES+DLSS+R VLSGAAPLGKELE ALR RVP A+ GQGYGMTE
Sbjct: 304  AAVVPPLVLALAKNPMVESFDLSSIRVVLSGAAPLGKELEAALRSRVPQAVLGQGYGMTE 363

Query: 1266 AGPVLSMCLGFAKEPTPMKFGSCGCVVRNAEMKIVDSDTGISLPRNKPGEICIRGAQIMK 1445
            AGPVLSMCLGFAK+P P K GSCG VVRNAE+K+VD +TG SL RN+PGEICIRG QIMK
Sbjct: 364  AGPVLSMCLGFAKQPFPTKSGSCGTVVRNAELKVVDPETGCSLGRNQPGEICIRGQQIMK 423

Query: 1446 GYLNDPDSTARTIDEEGWLHTGDIGYVDDDDEVFIIDRVKEIIKYKGFQVPPAELEALLI 1625
            GYLNDP++TA TID +GWLHTGDIGYVDDD+EVFI+DRVKE+IK+KGFQVPPAELEALL+
Sbjct: 424  GYLNDPEATASTIDVDGWLHTGDIGYVDDDEEVFIVDRVKELIKFKGFQVPPAELEALLV 483

Query: 1626 NHPSIADAAVVPLNDELAGEVPVAFLVKSENGPGVTEEDIKQFVAKQVVYYKRLHKVNFI 1805
            +HPSIADAAVVP  D++AGEVPVAF+V+S NG  +TEE +K+F++KQVV+YKRLHKV F+
Sbjct: 484  SHPSIADAAVVPQKDDVAGEVPVAFVVRS-NGFELTEEAVKEFISKQVVFYKRLHKVYFV 542

Query: 1806 HAIPKSPSGKILRKDLKAKLNEPIP 1880
            HAIPKSPSGKILRKDL+AKL E  P
Sbjct: 543  HAIPKSPSGKILRKDLRAKLAEKTP 567


>XP_002274994.1 PREDICTED: 4-coumarate--CoA ligase 2 [Vitis vinifera]
          Length = 570

 Score =  733 bits (1893), Expect = 0.0
 Identities = 368/565 (65%), Positives = 449/565 (79%), Gaps = 10/565 (1%)
 Frame = +3

Query: 216  EPESPSVKVLEKK---TEEIHKMEEYVFRSRLPDIYIPDNMSLHDYCFERLHELRDRPCL 386
            E ++P V  L+      +  ++++++VFRS+LP+I I +++ LH YCFE   +  DRPCL
Sbjct: 5    ETQNPDVSNLDTSHSIPKMANRIDDHVFRSKLPEIPISNHLPLHTYCFENYSQFADRPCL 64

Query: 387  IQGSTGKILTYGEVEASSRRVAAGLRKIGMAKGEVVMLLLQNCPEFVITFLGASMGGNVV 566
            I GST K  ++ E    SR+V AG   +G+ +G+VVM+LLQNC EF  +FLGASM G V 
Sbjct: 65   IVGSTNKTYSFAETHLISRKVGAGFAHLGLKQGDVVMILLQNCAEFAFSFLGASMVGAVT 124

Query: 567  TTANPFYTPADIAKQASASNTRIVVTSSSYVNKLGDLMSG-------GVRVFTVDEPQEG 725
            TTANPFYT A+I KQ +AS  +IVVT + YV+KL D   G       G  V T+D+P E 
Sbjct: 125  TTANPFYTSAEIFKQLNASKAKIVVTQAQYVDKLRDYPDGQVAKIGEGFTVITIDDPPEN 184

Query: 726  CEHFSVLTDVADEGPLGNVSVDAEDTVALPYSSGTTGLPKGVMLTHRSLVTSVAQQVDGD 905
            C HFSV+++ A+E  L  VS++++D VALP+SSGTTGLPKGV+LTH+SL+TSVAQQVDG+
Sbjct: 185  CMHFSVVSE-ANESELPEVSINSDDPVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGE 243

Query: 906  NPNLNLLPEDVVLCVLPLFHIYSLNSVLLCSLRAGSAVLLMHKFEIGNLLEFVPKYKISX 1085
            NPNL+L P+DVVLCVLPLFHIYSLNSVLLCSLRAG+AVLLM KFEIG LLE + +Y++S 
Sbjct: 244  NPNLHLTPDDVVLCVLPLFHIYSLNSVLLCSLRAGAAVLLMQKFEIGTLLELIQRYRVSV 303

Query: 1086 XXXXXXXXXXXXKNQMVESYDLSSVRFVLSGAAPLGKELEEALRKRVPNAIFGQGYGMTE 1265
                        KN MVES+DLSS+R VLSGAAPLGKELE ALR RVP A+ GQGYGMTE
Sbjct: 304  AAVVPPLVLALAKNPMVESFDLSSIRVVLSGAAPLGKELEAALRSRVPQAVLGQGYGMTE 363

Query: 1266 AGPVLSMCLGFAKEPTPMKFGSCGCVVRNAEMKIVDSDTGISLPRNKPGEICIRGAQIMK 1445
            AGPVLSMCL FAK+P P K GSCG VVRNAE+K+VD +TG SL RN+PGEICIRG QIMK
Sbjct: 364  AGPVLSMCLAFAKQPFPTKSGSCGTVVRNAELKVVDPETGCSLGRNQPGEICIRGQQIMK 423

Query: 1446 GYLNDPDSTARTIDEEGWLHTGDIGYVDDDDEVFIIDRVKEIIKYKGFQVPPAELEALLI 1625
            GYLNDP++TA TID +GWLHTGDIGYVDDD+EVFI+DRVKE+IK+KGFQVPPAELEALL+
Sbjct: 424  GYLNDPEATASTIDVDGWLHTGDIGYVDDDEEVFIVDRVKELIKFKGFQVPPAELEALLV 483

Query: 1626 NHPSIADAAVVPLNDELAGEVPVAFLVKSENGPGVTEEDIKQFVAKQVVYYKRLHKVNFI 1805
            +HPSIADAAVVP  D++AGEVPVAF+V+S NG  +TEE +K+F++KQVV+YKRLHKV F+
Sbjct: 484  SHPSIADAAVVPQKDDVAGEVPVAFVVRS-NGFELTEEAVKEFISKQVVFYKRLHKVYFV 542

Query: 1806 HAIPKSPSGKILRKDLKAKLNEPIP 1880
            HAIPKSPSGKILRKDL+AKL E  P
Sbjct: 543  HAIPKSPSGKILRKDLRAKLAEKTP 567


>XP_002520028.1 PREDICTED: 4-coumarate--CoA ligase 2 [Ricinus communis] EEF42352.1
            AMP dependent CoA ligase, putative [Ricinus communis]
          Length = 573

 Score =  726 bits (1874), Expect = 0.0
 Identities = 365/572 (63%), Positives = 445/572 (77%), Gaps = 9/572 (1%)
 Frame = +3

Query: 192  MATVPQITEP--ESPSVKVLEKKTEEIHKMEEYVFRSRLPDIYIPDNMSLHDYCFERLHE 365
            M ++  +  P  E  S    +K+       E ++FRS+LPDI I +++ LH YCFE L +
Sbjct: 1    MISIASLEPPKQELSSSSSSQKQNPSSSSSETHIFRSKLPDIPISNHLPLHTYCFENLFK 60

Query: 366  LRDRPCLIQGSTGKILTYGEVEASSRRVAAGLRKIGMAKGEVVMLLLQNCPEFVITFLGA 545
            L D+PCLI GSTGK  ++ E    S++ AAGL  +G+ KG+V+M+LLQNCPEFV +F+GA
Sbjct: 61   LSDKPCLISGSTGKTYSFAETHLISQKTAAGLSSLGIKKGDVIMILLQNCPEFVFSFMGA 120

Query: 546  SMGGNVVTTANPFYTPADIAKQASASNTRIVVTSSSYVNKLGD-------LMSGGVRVFT 704
            SM G V TTANPFYT  +I KQ ++S  ++++T S YV+KL D        +     V T
Sbjct: 121  SMIGAVATTANPFYTSNEIFKQLNSSRPKLIITQSLYVDKLRDPDHKIQPKLGEDFTVIT 180

Query: 705  VDEPQEGCEHFSVLTDVADEGPLGNVSVDAEDTVALPYSSGTTGLPKGVMLTHRSLVTSV 884
            +DEP E C HF+VL++ ++      ++ + +D VALP+SSGTTGLPKGV+LTH+SL+TSV
Sbjct: 181  IDEPPENCLHFTVLSEASETEIPEVINANPDDPVALPFSSGTTGLPKGVILTHKSLITSV 240

Query: 885  AQQVDGDNPNLNLLPEDVVLCVLPLFHIYSLNSVLLCSLRAGSAVLLMHKFEIGNLLEFV 1064
            AQQVDG+NPNL L  +DVVLCVLPLFHIYSLNSVLLCSLRAG+AVLLM KF+IG LLE +
Sbjct: 241  AQQVDGENPNLYLKEDDVVLCVLPLFHIYSLNSVLLCSLRAGAAVLLMQKFQIGALLELI 300

Query: 1065 PKYKISXXXXXXXXXXXXXKNQMVESYDLSSVRFVLSGAAPLGKELEEALRKRVPNAIFG 1244
             K+K+S             KN MV  +DLSS+R VLSGAAPLGKELE+ALR RVP AI G
Sbjct: 301  QKHKVSVAAVVPPLVLALAKNPMVAEFDLSSIRVVLSGAAPLGKELEDALRSRVPQAILG 360

Query: 1245 QGYGMTEAGPVLSMCLGFAKEPTPMKFGSCGCVVRNAEMKIVDSDTGISLPRNKPGEICI 1424
            QGYGMTEAGPVLSMCLGFAK+P P K GSCG VVRNAE+K++D +TG SL  N+PGEICI
Sbjct: 361  QGYGMTEAGPVLSMCLGFAKQPFPTKSGSCGTVVRNAELKVIDPETGCSLGYNQPGEICI 420

Query: 1425 RGAQIMKGYLNDPDSTARTIDEEGWLHTGDIGYVDDDDEVFIIDRVKEIIKYKGFQVPPA 1604
            RG QIMKGYLNDP++TA TID EGWLHTGDIGYVDDDDE+FI+DRVKEIIK+KGFQVPPA
Sbjct: 421  RGPQIMKGYLNDPEATANTIDVEGWLHTGDIGYVDDDDEIFIVDRVKEIIKFKGFQVPPA 480

Query: 1605 ELEALLINHPSIADAAVVPLNDELAGEVPVAFLVKSENGPGVTEEDIKQFVAKQVVYYKR 1784
            ELEALL+NHPSIADAAVVP  DE+AGEVPVAF+V+S NG  ++EED+K+++AKQVV+YK+
Sbjct: 481  ELEALLLNHPSIADAAVVPQKDEVAGEVPVAFVVRS-NGFELSEEDVKEYIAKQVVFYKK 539

Query: 1785 LHKVNFIHAIPKSPSGKILRKDLKAKLNEPIP 1880
            LHKV FIHAIPKSPSGKILRKDLKAKL  P P
Sbjct: 540  LHKVYFIHAIPKSPSGKILRKDLKAKLATPSP 571


>XP_018817126.1 PREDICTED: 4-coumarate--CoA ligase 2-like [Juglans regia]
          Length = 572

 Score =  724 bits (1868), Expect = 0.0
 Identities = 368/577 (63%), Positives = 453/577 (78%), Gaps = 8/577 (1%)
 Frame = +3

Query: 174  MIEVKCMATVPQITEPESPSVKVLEKKTEEIHKMEEYVFRSRLPDIYIPDNMSLHDYCFE 353
            M+ V   +++   T+  SP  K+   +  E  + +  VFRS+LPDI I +++ LH YCFE
Sbjct: 1    MVSVVSSSSIEPQTQDFSP--KISHSQNHEASETDR-VFRSKLPDIPISNHLPLHTYCFE 57

Query: 354  RLHELRDRPCLIQGSTGKILTYGEVEASSRRVAAGLRKIGMAKGEVVMLLLQNCPEFVIT 533
             L E  DRPCLI GSTGK  T+ E    S++VAAGL  +G+ KG+ +M+LLQNC EFV +
Sbjct: 58   HLSEFSDRPCLIVGSTGKSFTFAETHLISQKVAAGLFNLGIRKGDAIMILLQNCAEFVFS 117

Query: 534  FLGASMGGNVVTTANPFYTPADIAKQASASNTRIVVTSSSYVNKLGD------LMSGGVR 695
            F+GASM G V TTANPFYT A++ KQ  AS  +++++ S YV+KL +       +     
Sbjct: 118  FIGASMLGAVTTTANPFYTSAEVFKQFRASRAKLIISQSQYVDKLREPGEDYPKLGEDFT 177

Query: 696  VFTVDEPQEGCEHFSVLTDVADEGPLGNVSVDAEDTVALPYSSGTTGLPKGVMLTHRSLV 875
            V TVD+P E C HFSVL++ A+E  +  VS+D ED VALP+SSGTTGLPKGV+LTH+SL+
Sbjct: 178  VITVDDPPEKCLHFSVLSE-ANESEIPTVSIDPEDPVALPFSSGTTGLPKGVVLTHKSLI 236

Query: 876  TSVAQQVDGDNPNLNLLPEDVVLCVLPLFHIYSLNSVLLCSLRAGSAVLLMHKFEIGNLL 1055
            TSVAQQVDG+NPNL L PEDVVLCVLPLFHIYSLNSVLLCSLR G+AVLLM KFEIG+LL
Sbjct: 237  TSVAQQVDGENPNLYLKPEDVVLCVLPLFHIYSLNSVLLCSLRVGAAVLLMQKFEIGSLL 296

Query: 1056 EFVPKYKISXXXXXXXXXXXXXKNQMVESYDLSSVRFVLSGAAPLGKELEEALRKRVPNA 1235
            E + +++IS             KN +V+ +DLSS+R VLSGAAPLGKELEEAL+ R+P A
Sbjct: 297  ELIQRHRISVVAVVPPLVLALAKNPIVDKFDLSSIRVVLSGAAPLGKELEEALQSRIPQA 356

Query: 1236 IFGQGYGMTEAGPVLSMCLGFAKEPTPMKFGSCGCVVRNAEMKIVDSDTGISLPRNKPGE 1415
            IFGQGYGMTEAGPVLSMCLGFAK+P P K GSCG VVRNAE+K++D +TG S   N+PGE
Sbjct: 357  IFGQGYGMTEAGPVLSMCLGFAKQPFPTKSGSCGTVVRNAELKVIDPETGSSFGFNQPGE 416

Query: 1416 ICIRGAQIMKGYLNDPDSTARTIDEEGWLHTGDIGYVDDDDEVFIIDRVKEIIKYKGFQV 1595
            ICIRG+QIMKGYLNDP++TA TID+EGWLHTGDIGYVDDD E+FI+DRVKE+IK+KGFQV
Sbjct: 417  ICIRGSQIMKGYLNDPEATASTIDDEGWLHTGDIGYVDDDYEIFIVDRVKELIKFKGFQV 476

Query: 1596 PPAELEALLINHPSIADAAVVPLNDELAGEVPVAFLVKSENGPGVTEEDIKQFVAKQVVY 1775
            PPAELEALL++HPSIADAAVVP   ++AGEVPVAF+V+S NG  +TEE +K+FVAK+VV+
Sbjct: 477  PPAELEALLVSHPSIADAAVVPQKKDVAGEVPVAFVVRS-NGFELTEEAVKEFVAKEVVF 535

Query: 1776 YKRLHKVNFIHAIPKSPSGKILRKDLKAKL--NEPIP 1880
            YKRLHKV F+HAIPKSPSGKILRKDL+AKL  + P+P
Sbjct: 536  YKRLHKVYFVHAIPKSPSGKILRKDLRAKLATSSPLP 572


>AIS92506.1 4-coumarate:CoA ligase 2 [Epimedium sagittatum]
          Length = 568

 Score =  723 bits (1865), Expect = 0.0
 Identities = 364/560 (65%), Positives = 442/560 (78%), Gaps = 7/560 (1%)
 Frame = +3

Query: 216  EPESPSV--KVLEKKTEEIHKMEEYVFRSRLPDIYIPDNMSLHDYCFERLHELRDRPCLI 389
            E  +P V  +V+     E    E ++FRS+LPDI IP+++ LH YCFE + E  D+PCLI
Sbjct: 8    EANTPQVVPQVIPTSDVETPLSETFIFRSKLPDITIPNHLPLHTYCFENITEFADKPCLI 67

Query: 390  QGSTGKILTYGEVEASSRRVAAGLRKIGMAKGEVVMLLLQNCPEFVITFLGASMGGNVVT 569
             GS GKI T+ E     ++ AAGL K+G+ +G+VVM+LLQNCPEFV TF+GASM G + T
Sbjct: 68   SGSNGKIYTFEETHLICQKTAAGLSKLGVKEGDVVMILLQNCPEFVFTFMGASMLGAITT 127

Query: 570  TANPFYTPADIAKQASASNTRIVVTSSSYVNKLGD-----LMSGGVRVFTVDEPQEGCEH 734
            TANPFYT ++I KQ  AS T+++VT S YV+KL        +     V TVD+P E C H
Sbjct: 128  TANPFYTTSEIFKQYHASGTKLIVTQSHYVDKLRGGEDCPKIGEDFLVVTVDDPPENCIH 187

Query: 735  FSVLTDVADEGPLGNVSVDAEDTVALPYSSGTTGLPKGVMLTHRSLVTSVAQQVDGDNPN 914
            FSV+++ +DE  +  V++D++  VALP+SSGTTGLPKGV+LTH+SL++SVAQQVDG+NPN
Sbjct: 188  FSVVSE-SDEKEVPVVTIDSDTAVALPFSSGTTGLPKGVILTHKSLISSVAQQVDGENPN 246

Query: 915  LNLLPEDVVLCVLPLFHIYSLNSVLLCSLRAGSAVLLMHKFEIGNLLEFVPKYKISXXXX 1094
            L+L  ED+VLCVLPLFHIYSLNSVLLCSLRAG+ VL+M KFEIG+LLE + ++++S    
Sbjct: 247  LHLTTEDLVLCVLPLFHIYSLNSVLLCSLRAGAGVLIMPKFEIGSLLELIQRFRVSVAPV 306

Query: 1095 XXXXXXXXXKNQMVESYDLSSVRFVLSGAAPLGKELEEALRKRVPNAIFGQGYGMTEAGP 1274
                     KN MVE +DLSS+R VLSGAAPLGKELE AL+ RVP AIFGQGYGMTEAGP
Sbjct: 307  VPPLVLALAKNPMVEKFDLSSIRIVLSGAAPLGKELENALKSRVPQAIFGQGYGMTEAGP 366

Query: 1275 VLSMCLGFAKEPTPMKFGSCGCVVRNAEMKIVDSDTGISLPRNKPGEICIRGAQIMKGYL 1454
            VLSMC GFAK+P P K GSCG VVRNAE+KI+D +TG+SL  N PGEICIRG QIMKGYL
Sbjct: 367  VLSMCSGFAKQPFPTKSGSCGTVVRNAELKIIDPETGLSLKHNLPGEICIRGPQIMKGYL 426

Query: 1455 NDPDSTARTIDEEGWLHTGDIGYVDDDDEVFIIDRVKEIIKYKGFQVPPAELEALLINHP 1634
            ND DSTA TID EGWLHTGDIGYVDDD+EVFI+DRVKE+IK+KGFQVPPAELEALL+NHP
Sbjct: 427  NDADSTAATIDVEGWLHTGDIGYVDDDEEVFIVDRVKELIKFKGFQVPPAELEALLVNHP 486

Query: 1635 SIADAAVVPLNDELAGEVPVAFLVKSENGPGVTEEDIKQFVAKQVVYYKRLHKVNFIHAI 1814
            SIADAAVVP  D++ GEVPVAF+V S NG  ++E+ +K+F+AKQVV+YKRLHKV FIHAI
Sbjct: 487  SIADAAVVPQKDDMTGEVPVAFVVPS-NGFELSEDAVKEFIAKQVVFYKRLHKVYFIHAI 545

Query: 1815 PKSPSGKILRKDLKAKLNEP 1874
            PKSPSGKILRKDL+AKL  P
Sbjct: 546  PKSPSGKILRKDLRAKLASP 565


>XP_019199737.1 PREDICTED: 4-coumarate--CoA ligase 2 [Ipomoea nil]
          Length = 569

 Score =  723 bits (1865), Expect = 0.0
 Identities = 363/562 (64%), Positives = 437/562 (77%), Gaps = 6/562 (1%)
 Frame = +3

Query: 216  EPESPSVKVLEKKTEEIHKMEEYVFRSRLPDIYIPDNMSLHDYCFERLHELRDRPCLIQG 395
            E + P ++V +    +    + ++FRSRLPDI IP+ + LH YCF+ L E RDR CLI G
Sbjct: 5    ETQKPELEVADSGKTQAQSSQIHIFRSRLPDIPIPNQIPLHTYCFQNLAEYRDRTCLIVG 64

Query: 396  STGKILTYGEVEASSRRVAAGLRKIGMAKGEVVMLLLQNCPEFVITFLGASMGGNVVTTA 575
            STGK  ++ E     R+VA+GL K+G+ KG+V+M LLQNCPEFV +F+GASM G V TTA
Sbjct: 65   STGKTYSFAETHLICRKVASGLGKLGVKKGDVIMTLLQNCPEFVFSFMGASMIGAVTTTA 124

Query: 576  NPFYTPADIAKQASASNTRIVVTSSSYVNKLGDL------MSGGVRVFTVDEPQEGCEHF 737
            NPFYT ++I KQ SAS  ++++T S YV KL D       +     V TVD+P E C HF
Sbjct: 125  NPFYTKSEIFKQMSASKAKVIITQSMYVEKLKDSGEEYPKLGEDFSVVTVDDPPENCLHF 184

Query: 738  SVLTDVADEGPLGNVSVDAEDTVALPYSSGTTGLPKGVMLTHRSLVTSVAQQVDGDNPNL 917
            SVL++  +E     V +  +D VALP+SSGTTGLPKGV+LTH+SL+TSVAQQVDG+NPNL
Sbjct: 185  SVLSEADEEDIPATVDIAPDDAVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNL 244

Query: 918  NLLPEDVVLCVLPLFHIYSLNSVLLCSLRAGSAVLLMHKFEIGNLLEFVPKYKISXXXXX 1097
             L  +DVVLCVLPLFHI+SLNSVLLCSLRAG+AVLLM KFEI  LLE V K+++S     
Sbjct: 245  YLKEDDVVLCVLPLFHIFSLNSVLLCSLRAGAAVLLMQKFEIKALLELVKKHRVSVAAVV 304

Query: 1098 XXXXXXXXKNQMVESYDLSSVRFVLSGAAPLGKELEEALRKRVPNAIFGQGYGMTEAGPV 1277
                    KN +V+SYDLSS+R VLSGAAPLGKELEEAL +RVP AIFGQGYGMTEAGPV
Sbjct: 305  PPLVLALAKNPIVDSYDLSSIRVVLSGAAPLGKELEEALLQRVPQAIFGQGYGMTEAGPV 364

Query: 1278 LSMCLGFAKEPTPMKFGSCGCVVRNAEMKIVDSDTGISLPRNKPGEICIRGAQIMKGYLN 1457
            LSMC  FAK+P P K GSCG VVRNAE+ +VD +TG SL RNKPGEICIRG+QIMK YLN
Sbjct: 365  LSMCPAFAKQPLPAKSGSCGSVVRNAELMVVDPETGCSLGRNKPGEICIRGSQIMKEYLN 424

Query: 1458 DPDSTARTIDEEGWLHTGDIGYVDDDDEVFIIDRVKEIIKYKGFQVPPAELEALLINHPS 1637
            DP +TARTID +GWLHTGDIG+VDDDDEVFI+DRVKE+IK+KGFQVPPAELEALL++HP 
Sbjct: 425  DPAATARTIDVDGWLHTGDIGFVDDDDEVFIVDRVKELIKFKGFQVPPAELEALLLSHPM 484

Query: 1638 IADAAVVPLNDELAGEVPVAFLVKSENGPGVTEEDIKQFVAKQVVYYKRLHKVNFIHAIP 1817
            IADAAVVP  D+ AGEVPVAF+V+S +G  +TEE +K+F+AKQVV+YK+LHKV FIHAIP
Sbjct: 485  IADAAVVPQKDDAAGEVPVAFVVRSSDGFDLTEEAVKEFIAKQVVFYKKLHKVYFIHAIP 544

Query: 1818 KSPSGKILRKDLKAKLNEPIPN 1883
            KS SGKILRKDL+ KL  P P+
Sbjct: 545  KSASGKILRKDLREKLQAPPPS 566


>XP_010034948.2 PREDICTED: LOW QUALITY PROTEIN: 4-coumarate--CoA ligase 2 [Eucalyptus
            grandis]
          Length = 570

 Score =  723 bits (1865), Expect = 0.0
 Identities = 364/566 (64%), Positives = 445/566 (78%), Gaps = 14/566 (2%)
 Frame = +3

Query: 225  SPSVKVLEKKTEEIHKMEEY-------VFRSRLPDIYIPDNMSLHDYCFERLHELRDRPC 383
            +PS +   K   E+ + E+        +FRS+LPDI IP+++SLH YCFE + +  DRPC
Sbjct: 5    APSPETAPKNPSEVPQGEKREAPAACTIFRSKLPDIPIPNHLSLHSYCFEHVPQFPDRPC 64

Query: 384  LIQGSTGKILTYGEVEASSRRVAAGLRKIGMAKGEVVMLLLQNCPEFVITFLGASMGGNV 563
            LI  STGK  ++ +    SR+VAAGL  +G+ +G+VVM LLQNCPEF  +F+GASM G V
Sbjct: 65   LISDSTGKSFSFSDALLLSRKVAAGLHNLGIRRGDVVMTLLQNCPEFAFSFMGASMIGAV 124

Query: 564  VTTANPFYTPADIAKQASASNTRIVVTSSSYVNKLGDLMSGG-------VRVFTVDEPQE 722
             TTANPFYTP++I KQ +AS T++V+T S YV+KL D   G          V TVD+P E
Sbjct: 125  TTTANPFYTPSEIFKQFAASRTKLVITQSLYVDKLRDHADGNGPTLGKDFVVVTVDDPPE 184

Query: 723  GCEHFSVLTDVADEGPLGNVSVDAEDTVALPYSSGTTGLPKGVMLTHRSLVTSVAQQVDG 902
            GC HFSVL + A+E     V++  +D VALP+SSGTTGLPKGV+LTHR+L+TS+AQQVDG
Sbjct: 185  GCVHFSVLGE-ANEEEAPEVAIHPDDPVALPFSSGTTGLPKGVILTHRNLITSIAQQVDG 243

Query: 903  DNPNLNLLPEDVVLCVLPLFHIYSLNSVLLCSLRAGSAVLLMHKFEIGNLLEFVPKYKIS 1082
            +NPNL+L  EDV+LCVLPLFHIYSLNSVLLCSLRAG+ VLLMHKFEIG LL+ + ++++S
Sbjct: 244  ENPNLHLRAEDVMLCVLPLFHIYSLNSVLLCSLRAGAGVLLMHKFEIGTLLQLIERHRVS 303

Query: 1083 XXXXXXXXXXXXXKNQMVESYDLSSVRFVLSGAAPLGKELEEALRKRVPNAIFGQGYGMT 1262
                         KN +VE +DLSS+R VLSGAAPLGKELE AL+ R+P AI GQGYGMT
Sbjct: 304  VAAVVPPLVLALAKNPLVEKFDLSSIRMVLSGAAPLGKELELALQTRLPGAILGQGYGMT 363

Query: 1263 EAGPVLSMCLGFAKEPTPMKFGSCGCVVRNAEMKIVDSDTGISLPRNKPGEICIRGAQIM 1442
            EAGPVLSMCLGFAK+P P K GSCG VVRNAE+K++D +TG SL  N+PGEICIRG QIM
Sbjct: 364  EAGPVLSMCLGFAKQPFPTKSGSCGTVVRNAELKVIDPETGSSLGYNQPGEICIRGQQIM 423

Query: 1443 KGYLNDPDSTARTIDEEGWLHTGDIGYVDDDDEVFIIDRVKEIIKYKGFQVPPAELEALL 1622
            KGYLNDP++T+ TID +GWLHTGDIGYVDDDDE+FI+DRVKEIIK+KGFQVPPAELEALL
Sbjct: 424  KGYLNDPEATSITIDADGWLHTGDIGYVDDDDEIFIVDRVKEIIKFKGFQVPPAELEALL 483

Query: 1623 INHPSIADAAVVPLNDELAGEVPVAFLVKSENGPGVTEEDIKQFVAKQVVYYKRLHKVNF 1802
            ++HPSIADAAVVP  DE+AGEVPVAF+V+S NG   TEE +K+F+AKQVV+YK+LHKV+F
Sbjct: 484  VSHPSIADAAVVPQKDEVAGEVPVAFVVRS-NGFDXTEEAVKEFIAKQVVFYKKLHKVHF 542

Query: 1803 IHAIPKSPSGKILRKDLKAKLNEPIP 1880
            +HAIPKSPSGKILRKDL+AKL    P
Sbjct: 543  VHAIPKSPSGKILRKDLRAKLATAAP 568


>XP_010261545.1 PREDICTED: 4-coumarate--CoA ligase 2 isoform X1 [Nelumbo nucifera]
          Length = 574

 Score =  722 bits (1864), Expect = 0.0
 Identities = 361/569 (63%), Positives = 448/569 (78%), Gaps = 11/569 (1%)
 Frame = +3

Query: 192  MATVPQITEPESPSVKVLEKKTEEIHKM-----EEYVFRSRLPDIYIPDNMSLHDYCFER 356
            MAT+  +  PE+   ++    T  IH       E ++FRS+LPDI I +++ LH +CFE+
Sbjct: 1    MATMVSVASPETQPPQI----TPSIHPTKDPATETFIFRSKLPDIPISNHLPLHTFCFEK 56

Query: 357  LHELRDRPCLIQGSTGKILTYGEVEASSRRVAAGLRKIGMAKGEVVMLLLQNCPEFVITF 536
            + E  DRPCLI GS G+I T+ E     +++A GL K G+ KG+V+M+LLQN PEFV +F
Sbjct: 57   VSEFSDRPCLITGSNGRIYTFAETHLICQKIAVGLSKFGIKKGDVIMVLLQNSPEFVFSF 116

Query: 537  LGASMGGNVVTTANPFYTPADIAKQASASNTRIVVTSSSYVNKLGD------LMSGGVRV 698
            +GASM G V TTANPFYTPA+I KQ S S  ++++T S YV+KL D       +  G+ V
Sbjct: 117  MGASMLGAVTTTANPFYTPAEIYKQFSTSXAKLIITQSQYVDKLKDPSQKFPKIGEGLTV 176

Query: 699  FTVDEPQEGCEHFSVLTDVADEGPLGNVSVDAEDTVALPYSSGTTGLPKGVMLTHRSLVT 878
             TVD+P E C HFSV+++ +DE  L +VS+D +D VALP+SSGTTG PKGV+LTH+SL +
Sbjct: 177  VTVDDPPENCVHFSVISN-SDERELPSVSIDPDDPVALPFSSGTTGFPKGVILTHKSLTS 235

Query: 879  SVAQQVDGDNPNLNLLPEDVVLCVLPLFHIYSLNSVLLCSLRAGSAVLLMHKFEIGNLLE 1058
            SVAQQVDG+NPNL+L  EDVVLCVLPLFHIYSLNSVLLCSLRA +AVLLM KFEIG LL+
Sbjct: 236  SVAQQVDGENPNLHLKTEDVVLCVLPLFHIYSLNSVLLCSLRARAAVLLMQKFEIGALLD 295

Query: 1059 FVPKYKISXXXXXXXXXXXXXKNQMVESYDLSSVRFVLSGAAPLGKELEEALRKRVPNAI 1238
             + +++++             KNQMVE++DLSS+R VLSGAAPLGKELEEAL  RVP A+
Sbjct: 296  LIQRHRVTVAPVVPPLVLALAKNQMVENFDLSSIRIVLSGAAPLGKELEEALHSRVPQAV 355

Query: 1239 FGQGYGMTEAGPVLSMCLGFAKEPTPMKFGSCGCVVRNAEMKIVDSDTGISLPRNKPGEI 1418
            FGQGYGMTEAGPVLSMCLGFAK+P P K GSCG VVRNAE+K++D  TG SLP N+PG+I
Sbjct: 356  FGQGYGMTEAGPVLSMCLGFAKQPFPTKSGSCGTVVRNAELKVIDPATGASLPHNQPGQI 415

Query: 1419 CIRGAQIMKGYLNDPDSTARTIDEEGWLHTGDIGYVDDDDEVFIIDRVKEIIKYKGFQVP 1598
            CIRG QIMKGYLNDPD+TA TID +GWLHTGDIGYVDDD+EVFI+DRVKE+IK+KGFQVP
Sbjct: 416  CIRGPQIMKGYLNDPDATASTIDVDGWLHTGDIGYVDDDEEVFIVDRVKELIKFKGFQVP 475

Query: 1599 PAELEALLINHPSIADAAVVPLNDELAGEVPVAFLVKSENGPGVTEEDIKQFVAKQVVYY 1778
            PAELEALL++HP I+DAA+VP  D +AGE+PVAF+V+S NG  +TEE +K+F+AKQVV+Y
Sbjct: 476  PAELEALLVSHPFISDAAIVPQKDAVAGEIPVAFVVRS-NGFDLTEEAVKEFIAKQVVFY 534

Query: 1779 KRLHKVNFIHAIPKSPSGKILRKDLKAKL 1865
            K+LHKV F+ AIPKSPSGKILRKDL+AKL
Sbjct: 535  KKLHKVYFVPAIPKSPSGKILRKDLRAKL 563


>AFG26323.1 4-coumarate-CoA ligase [Cinnamomum osmophloeum]
          Length = 539

 Score =  720 bits (1858), Expect = 0.0
 Identities = 350/537 (65%), Positives = 431/537 (80%), Gaps = 1/537 (0%)
 Frame = +3

Query: 276  EEYVFRSRLPDIYIPDNMSLHDYCFERLHELRDRPCLIQGSTGKILTYGEVEASSRRVAA 455
            ++++F+S+LPDIYIP+++ LH YCF+ +H   DRPC+I G+TG I TY +VE +SR+VAA
Sbjct: 5    KDHIFKSKLPDIYIPNHIPLHSYCFQNIHHFADRPCIIDGATGDIFTYADVELTSRKVAA 64

Query: 456  GLRKIGMAKGEVVMLLLQNCPEFVITFLGASMGGNVVTTANPFYTPADIAKQASASNTRI 635
            GL  +G+ KG+V+M+LL NCPEFV  FLGAS  G   TTANPFYTP +++KQA A N  +
Sbjct: 65   GLHHLGVEKGDVIMILLPNCPEFVFAFLGASYRGATTTTANPFYTPQEVSKQAKACNACV 124

Query: 636  VVTSSSYVNKLGDLMS-GGVRVFTVDEPQEGCEHFSVLTDVADEGPLGNVSVDAEDTVAL 812
            V+T S YV+KL DLM    V+V  +D+  EGC HFS L + ADE  L  V +  +D VAL
Sbjct: 125  VITQSQYVDKLRDLMQESDVKVVCIDKAVEGCMHFSELAE-ADESELPEVDISPDDVVAL 183

Query: 813  PYSSGTTGLPKGVMLTHRSLVTSVAQQVDGDNPNLNLLPEDVVLCVLPLFHIYSLNSVLL 992
            PYSSGTTGLPKGVMLTH+ L+TSVAQQVDGDNPNL    EDVVLC+LPLFHIYSLNSVLL
Sbjct: 184  PYSSGTTGLPKGVMLTHKGLITSVAQQVDGDNPNLYFKKEDVVLCLLPLFHIYSLNSVLL 243

Query: 993  CSLRAGSAVLLMHKFEIGNLLEFVPKYKISXXXXXXXXXXXXXKNQMVESYDLSSVRFVL 1172
            C LR G+A+L+M KF+I  L+E V KYK++             K+  V+ YDLSS+R V+
Sbjct: 244  CGLRVGAAILIMQKFDIVALMELVQKYKVTIAPFVPPIVLAIAKSPEVDRYDLSSIRTVM 303

Query: 1173 SGAAPLGKELEEALRKRVPNAIFGQGYGMTEAGPVLSMCLGFAKEPTPMKFGSCGCVVRN 1352
            SGAAP+GKELE+ LR ++PNA  GQGYGMTEAGPVLSMCL FAKEP  +K GSCG VVRN
Sbjct: 304  SGAAPMGKELEDILRAKIPNAKLGQGYGMTEAGPVLSMCLAFAKEPFEIKSGSCGTVVRN 363

Query: 1353 AEMKIVDSDTGISLPRNKPGEICIRGAQIMKGYLNDPDSTARTIDEEGWLHTGDIGYVDD 1532
            AE+KIVD +TG SLPRN+ GEICIRG+QIMKGYLNDP++T  TID+EGWLHTGDIG+VDD
Sbjct: 364  AELKIVDPETGASLPRNQAGEICIRGSQIMKGYLNDPEATKMTIDKEGWLHTGDIGFVDD 423

Query: 1533 DDEVFIIDRVKEIIKYKGFQVPPAELEALLINHPSIADAAVVPLNDELAGEVPVAFLVKS 1712
            DDE+FI+DR+KE+IKYKGFQV PAELEA+LI HP++ADAAVVP+ D LAGEVPVAF+VKS
Sbjct: 424  DDEIFIVDRLKELIKYKGFQVAPAELEAMLITHPNVADAAVVPMKDVLAGEVPVAFVVKS 483

Query: 1713 ENGPGVTEEDIKQFVAKQVVYYKRLHKVNFIHAIPKSPSGKILRKDLKAKLNEPIPN 1883
             N   +TEE+IKQFVAKQVV+YKR+++V F+ +IPK+PSGKILRKDL+A+L   +PN
Sbjct: 484  -NSSNITEEEIKQFVAKQVVFYKRINRVFFVDSIPKAPSGKILRKDLRARLAAGMPN 539


>AMN10098.1 4-coumarate-CoA ligase [Ginkgo biloba]
          Length = 557

 Score =  719 bits (1857), Expect = 0.0
 Identities = 352/550 (64%), Positives = 440/550 (80%)
 Frame = +3

Query: 219  PESPSVKVLEKKTEEIHKMEEYVFRSRLPDIYIPDNMSLHDYCFERLHELRDRPCLIQGS 398
            P +   KV     +  +  +EY+FRS+LPDI IP ++ LH YCFERL E  ++PCLI G+
Sbjct: 6    PSTFDHKVTSAPAKMENGADEYLFRSKLPDIQIPYHLPLHTYCFERLSEFAEKPCLIDGA 65

Query: 399  TGKILTYGEVEASSRRVAAGLRKIGMAKGEVVMLLLQNCPEFVITFLGASMGGNVVTTAN 578
            TGKI  YGEVE  SR+VAAGL K+G+ +GEVVMLLLQNC EF   FLG+S+ G + TTAN
Sbjct: 66   TGKIYNYGEVELISRKVAAGLAKLGLKQGEVVMLLLQNCVEFAFVFLGSSIRGAITTTAN 125

Query: 579  PFYTPADIAKQASASNTRIVVTSSSYVNKLGDLMSGGVRVFTVDEPQEGCEHFSVLTDVA 758
            PFYTP +IAKQA+AS  RI+VT ++Y +KL DL S  + V T+D P EGC+H SVLT+ A
Sbjct: 126  PFYTPGEIAKQANASGARIIVTQAAYADKLTDLKSENLIVITIDSPPEGCQHISVLTE-A 184

Query: 759  DEGPLGNVSVDAEDTVALPYSSGTTGLPKGVMLTHRSLVTSVAQQVDGDNPNLNLLPEDV 938
            DE    +V++D +D VALPYSSG TGLPKGVMLTH+  V+SVAQQVDG+NPNL +  EDV
Sbjct: 185  DESECPSVNIDPDDVVALPYSSGATGLPKGVMLTHKGCVSSVAQQVDGENPNLYMHSEDV 244

Query: 939  VLCVLPLFHIYSLNSVLLCSLRAGSAVLLMHKFEIGNLLEFVPKYKISXXXXXXXXXXXX 1118
            +LCVLPLFHIYSLN+ LLC+LRAG+A+L+M KF    LLE + ++K++            
Sbjct: 245  ILCVLPLFHIYSLNAALLCALRAGAAILIMQKFNTVALLELIQRFKVTIAPFVPPIVLEM 304

Query: 1119 XKNQMVESYDLSSVRFVLSGAAPLGKELEEALRKRVPNAIFGQGYGMTEAGPVLSMCLGF 1298
             KN +V +YD+SS+R ++SG APLGKELE+ALR R+P A FGQGYGMTEAGPV +M L F
Sbjct: 305  AKNPIVLNYDVSSIRVIMSGGAPLGKELEDALRARLPKAKFGQGYGMTEAGPVPAMNLAF 364

Query: 1299 AKEPTPMKFGSCGCVVRNAEMKIVDSDTGISLPRNKPGEICIRGAQIMKGYLNDPDSTAR 1478
            AKEP P+K G+CG VV NA+MKI+D++TG  LPRNKPGEICIRG QIMKGYLNDP++TAR
Sbjct: 365  AKEPYPVKSGACGTVVPNAQMKIIDTETGQCLPRNKPGEICIRGPQIMKGYLNDPEATAR 424

Query: 1479 TIDEEGWLHTGDIGYVDDDDEVFIIDRVKEIIKYKGFQVPPAELEALLINHPSIADAAVV 1658
            TIDEEGWLHTGD+G++D+D+E+FI+DRVKE+IKYKGFQV PAELEA+L+NHP IADAAVV
Sbjct: 425  TIDEEGWLHTGDVGFIDNDEEIFIVDRVKELIKYKGFQVAPAELEAILVNHPFIADAAVV 484

Query: 1659 PLNDELAGEVPVAFLVKSENGPGVTEEDIKQFVAKQVVYYKRLHKVNFIHAIPKSPSGKI 1838
            P  +E AGE+PVAF+VKS NG  ++E++IK+FVAKQVV+YK++ KV F+ AIPKSPSGKI
Sbjct: 485  PQKNEAAGEIPVAFVVKS-NGAEISEQEIKEFVAKQVVFYKKIQKVFFLDAIPKSPSGKI 543

Query: 1839 LRKDLKAKLN 1868
            LRKDL+A+LN
Sbjct: 544  LRKDLRARLN 553


>XP_008234565.1 PREDICTED: 4-coumarate--CoA ligase 2 [Prunus mume]
          Length = 606

 Score =  721 bits (1862), Expect = 0.0
 Identities = 357/547 (65%), Positives = 437/547 (79%), Gaps = 14/547 (2%)
 Frame = +3

Query: 282  YVFRSRLPDIYIPDNMSLHDYCFERLHELRDRPCLIQGSTGKILTYGEVEASSRRVAAGL 461
            +VF+S+LPDI IP+++ LH YCF++L E  DRPCLI GSTGK  ++ +    S++  AGL
Sbjct: 60   HVFKSKLPDIPIPNHLPLHTYCFQKLSEFSDRPCLIVGSTGKSYSFSDTYLISQKTGAGL 119

Query: 462  RKIGMAKGEVVMLLLQNCPEFVITFLGASMGGNVVTTANPFYTPADIAKQASASNTRIVV 641
              +G+ KG+V+M+LLQNC EFV  F+GASM G V TTANPFYT A+I KQ  ASN ++++
Sbjct: 120  SNLGIKKGDVIMILLQNCAEFVFAFMGASMIGAVTTTANPFYTAAEIFKQFKASNAKLII 179

Query: 642  TSSSYVNKLGDLMSGGV--------------RVFTVDEPQEGCEHFSVLTDVADEGPLGN 779
            T S +V+KL +  + G               +V TVD+P E C HFSV+++ A+E  L  
Sbjct: 180  THSQFVDKLREAPADGAAPDQNNYPKLGEHFKVVTVDDPPENCLHFSVISE-ANETELPE 238

Query: 780  VSVDAEDTVALPYSSGTTGLPKGVMLTHRSLVTSVAQQVDGDNPNLNLLPEDVVLCVLPL 959
            V +D ED VALP+SSGTTGLPKGV+LTH+SL+TSVAQQVDG+NPNL L  +DVVLCVLPL
Sbjct: 239  VVIDPEDPVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLKEDDVVLCVLPL 298

Query: 960  FHIYSLNSVLLCSLRAGSAVLLMHKFEIGNLLEFVPKYKISXXXXXXXXXXXXXKNQMVE 1139
            FHIYSLNSV+LCSLRAG+AVL+MHKFEIG LLE + +Y++S             KN MV 
Sbjct: 299  FHIYSLNSVMLCSLRAGAAVLVMHKFEIGTLLELIQRYRVSVAAVVPPLVIALAKNPMVA 358

Query: 1140 SYDLSSVRFVLSGAAPLGKELEEALRKRVPNAIFGQGYGMTEAGPVLSMCLGFAKEPTPM 1319
             +DLSS+R VLSGAAPLGKELEEAL+ RVP A+ GQGYGMTEAGPVLSMCL FAKEP P 
Sbjct: 359  QFDLSSIRVVLSGAAPLGKELEEALKGRVPRAVLGQGYGMTEAGPVLSMCLAFAKEPLPS 418

Query: 1320 KFGSCGCVVRNAEMKIVDSDTGISLPRNKPGEICIRGAQIMKGYLNDPDSTARTIDEEGW 1499
            K GSCG VVRNAE+K++D++TG SL  N+PGEICIRG+QIMKGYLND ++TA T+D+EGW
Sbjct: 419  KSGSCGTVVRNAELKVIDAETGCSLGYNQPGEICIRGSQIMKGYLNDAEATATTVDKEGW 478

Query: 1500 LHTGDIGYVDDDDEVFIIDRVKEIIKYKGFQVPPAELEALLINHPSIADAAVVPLNDELA 1679
            LHTGD+GYVDDD+EVFI+DRVKE+IK+KGFQVPPAELE+LL++HPSIADAAVVP  DE A
Sbjct: 479  LHTGDVGYVDDDEEVFIVDRVKELIKFKGFQVPPAELESLLVSHPSIADAAVVPQKDEAA 538

Query: 1680 GEVPVAFLVKSENGPGVTEEDIKQFVAKQVVYYKRLHKVNFIHAIPKSPSGKILRKDLKA 1859
            GEVPVAF+V+S NG  +TEE +K+F+AKQVV+YKRLHKV F+HAIPKSPSGKILRKDL+A
Sbjct: 539  GEVPVAFVVRS-NGLQLTEEAVKEFIAKQVVFYKRLHKVYFVHAIPKSPSGKILRKDLRA 597

Query: 1860 KLNEPIP 1880
            KL  P P
Sbjct: 598  KLATPTP 604


>AKG06666.1 4-coumarate-CoA ligase 2 [Ziziphus jujuba]
          Length = 591

 Score =  718 bits (1854), Expect = 0.0
 Identities = 366/571 (64%), Positives = 444/571 (77%), Gaps = 8/571 (1%)
 Frame = +3

Query: 195  ATVPQITEPESPSVKVLEKKTEEIHKMEEYVFRSRLPDIYIPDNMSLHDYCFER-LHELR 371
            AT P    P  P    L ++ + IH     VF+S+LPDI I +++ LH YCFER L E  
Sbjct: 28   ATTPP--PPPPPPKNQLPQQDDAIH-----VFKSKLPDIPISNHVPLHTYCFERSLVEFP 80

Query: 372  DRPCLIQGSTGKILTYGEVEASSRRVAAGLRKIGMAKGEVVMLLLQNCPEFVITFLGASM 551
            DRPCLI GSTGK  T+ E    S ++AAGL  +G+ KG+V+M+LLQNC EFV  F+GAS+
Sbjct: 81   DRPCLIVGSTGKTYTFAETHLISSKIAAGLSTLGIKKGDVIMILLQNCAEFVFAFMGASI 140

Query: 552  GGNVVTTANPFYTPADIAKQASASNTRIVVTSSSYVNKLGDLMSG-------GVRVFTVD 710
             G V TTANPFYT  +I KQ +AS +++++T S YV+KL +   G         +V T+D
Sbjct: 141  IGAVSTTANPFYTVPEIFKQFNASKSKLIITQSQYVDKLREPGGGQYPKLGQDFKVITID 200

Query: 711  EPQEGCEHFSVLTDVADEGPLGNVSVDAEDTVALPYSSGTTGLPKGVMLTHRSLVTSVAQ 890
            +P E C  FSV++D A+E     V +DAED VALP+SSGTTGLPKGV+LTH+SL+TSVAQ
Sbjct: 201  DPPENCLDFSVISD-ANENEFPEVVIDAEDPVALPFSSGTTGLPKGVVLTHKSLITSVAQ 259

Query: 891  QVDGDNPNLNLLPEDVVLCVLPLFHIYSLNSVLLCSLRAGSAVLLMHKFEIGNLLEFVPK 1070
            QVDGDNPNL L  EDVV CVLP+FHI+SLNSVLL SLR G+AVL+MHKFEIG LLE + +
Sbjct: 260  QVDGDNPNLYLTEEDVVPCVLPMFHIFSLNSVLLNSLRVGAAVLIMHKFEIGALLELIQR 319

Query: 1071 YKISXXXXXXXXXXXXXKNQMVESYDLSSVRFVLSGAAPLGKELEEALRKRVPNAIFGQG 1250
            +++S             KN MV+ +DLSS+R VLSGAAPLGKELEEALR RVP A+ GQG
Sbjct: 320  HRVSVAAVVPPLVLALAKNPMVDKFDLSSIRLVLSGAAPLGKELEEALRNRVPQAVLGQG 379

Query: 1251 YGMTEAGPVLSMCLGFAKEPTPMKFGSCGCVVRNAEMKIVDSDTGISLPRNKPGEICIRG 1430
            YGMTEAGPVLSMCLGFAK+P P K GSCG VVRNAE+K+VD ++G SL  N+PGEICIRG
Sbjct: 380  YGMTEAGPVLSMCLGFAKQPFPTKSGSCGTVVRNAELKVVDPESGCSLGYNQPGEICIRG 439

Query: 1431 AQIMKGYLNDPDSTARTIDEEGWLHTGDIGYVDDDDEVFIIDRVKEIIKYKGFQVPPAEL 1610
            +QIMKGYLND ++TA TIDE+GWLHTGDIGYVDDDDE+FI+DRVKEIIK+KGFQVPPAEL
Sbjct: 440  SQIMKGYLNDAEATANTIDEDGWLHTGDIGYVDDDDEIFIVDRVKEIIKFKGFQVPPAEL 499

Query: 1611 EALLINHPSIADAAVVPLNDELAGEVPVAFLVKSENGPGVTEEDIKQFVAKQVVYYKRLH 1790
            EALL+ HPSIADAAVVP NDE+AGEVPVAF+V+S NG  +TEE +K+F++KQVV+YKRLH
Sbjct: 500  EALLVGHPSIADAAVVPQNDEVAGEVPVAFVVRSTNGVELTEEAVKEFISKQVVFYKRLH 559

Query: 1791 KVNFIHAIPKSPSGKILRKDLKAKLNEPIPN 1883
            KV F+HA+PKSPSGKILRKDL+AKL    P+
Sbjct: 560  KVYFVHAVPKSPSGKILRKDLRAKLATASPS 590


>XP_016202253.1 PREDICTED: 4-coumarate--CoA ligase 2 [Arachis ipaensis]
          Length = 574

 Score =  717 bits (1852), Expect = 0.0
 Identities = 357/532 (67%), Positives = 430/532 (80%), Gaps = 4/532 (0%)
 Frame = +3

Query: 282  YVFRSRLPDIYIPDNMSLHDYCFERLHELRDRPCLIQGSTGKILTYGEVEASSRRVAAGL 461
            ++F+S+LPDI + +++ LH +CF+RL+E+ DRPCLI GS GK  TYGE   +SRR+AAG+
Sbjct: 37   HIFKSKLPDIPLSNHLPLHSFCFQRLNEISDRPCLIVGSAGKTYTYGETYHASRRLAAGM 96

Query: 462  RKIGMAKGEVVMLLLQNCPEFVITFLGASMGGNVVTTANPFYTPADIAKQASASNTRIVV 641
              IG+ KG+V+M+LLQN  EFV +F+ ASM G V TTANPFYTPA+I KQ +AS  ++V+
Sbjct: 97   SAIGITKGDVIMILLQNSAEFVFSFIAASMIGAVATTANPFYTPAEIFKQITASKAKLVI 156

Query: 642  TSSSYVNKLGDLMSGGV----RVFTVDEPQEGCEHFSVLTDVADEGPLGNVSVDAEDTVA 809
            T + YV+KL D  S  +    +V TVD+P E C HFS +++  +E  L  V +  +D VA
Sbjct: 157  TQAMYVDKLRDSDSAKIGEDFKVVTVDDPPENCLHFSAISE-GNENDLPEVEIHPDDAVA 215

Query: 810  LPYSSGTTGLPKGVMLTHRSLVTSVAQQVDGDNPNLNLLPEDVVLCVLPLFHIYSLNSVL 989
            LP+SSGTTGLPKGV+LTHRSL TSVAQQVDG+NPNL L  EDV+LCVLPLFHI+SLNSVL
Sbjct: 216  LPFSSGTTGLPKGVVLTHRSLTTSVAQQVDGENPNLYLKTEDVLLCVLPLFHIFSLNSVL 275

Query: 990  LCSLRAGSAVLLMHKFEIGNLLEFVPKYKISXXXXXXXXXXXXXKNQMVESYDLSSVRFV 1169
            LC+LRAGSAVLLM KF+IG LLE + +Y++S             KN MV  YDLSS+R V
Sbjct: 276  LCALRAGSAVLLMQKFDIGTLLELIQRYRVSVAMVVPPLVLALAKNPMVAEYDLSSIRLV 335

Query: 1170 LSGAAPLGKELEEALRKRVPNAIFGQGYGMTEAGPVLSMCLGFAKEPTPMKFGSCGCVVR 1349
            LSGAAPLGKELEEALR RVP A+ GQGYGMTEAGPVLSMCLGFAK+P P K GSCG VVR
Sbjct: 336  LSGAAPLGKELEEALRSRVPQAVLGQGYGMTEAGPVLSMCLGFAKQPFPTKSGSCGTVVR 395

Query: 1350 NAEMKIVDSDTGISLPRNKPGEICIRGAQIMKGYLNDPDSTARTIDEEGWLHTGDIGYVD 1529
            NAE+K+VD +TG SL  N+PGEICIRG QIMKGYLND  +TA TID +GWLHTGD+GYVD
Sbjct: 396  NAELKVVDPETGCSLGYNQPGEICIRGQQIMKGYLNDDTATATTIDVDGWLHTGDVGYVD 455

Query: 1530 DDDEVFIIDRVKEIIKYKGFQVPPAELEALLINHPSIADAAVVPLNDELAGEVPVAFLVK 1709
            DDDE+FI+DRVKE+IK+KGFQVPPAELEALL++HPSIADAAVVP  D  AGEVPVAF+V+
Sbjct: 456  DDDEIFIVDRVKELIKFKGFQVPPAELEALLVSHPSIADAAVVPQKDVAAGEVPVAFVVR 515

Query: 1710 SENGPGVTEEDIKQFVAKQVVYYKRLHKVNFIHAIPKSPSGKILRKDLKAKL 1865
            S NG  +TEE +K+F+AKQVV+YKRLHKV F+HAIPKSPSGKILRKDL+AKL
Sbjct: 516  S-NGFELTEESVKEFIAKQVVFYKRLHKVYFVHAIPKSPSGKILRKDLRAKL 566


>XP_015964696.1 PREDICTED: 4-coumarate--CoA ligase 2 [Arachis duranensis]
          Length = 574

 Score =  717 bits (1852), Expect = 0.0
 Identities = 357/532 (67%), Positives = 430/532 (80%), Gaps = 4/532 (0%)
 Frame = +3

Query: 282  YVFRSRLPDIYIPDNMSLHDYCFERLHELRDRPCLIQGSTGKILTYGEVEASSRRVAAGL 461
            ++F+S+LPDI + +++ LH +CF+RL+E+ DRPCLI GS GKI TYGE   +SRR+AAG+
Sbjct: 37   HIFKSKLPDIPLSNHLPLHSFCFQRLNEISDRPCLIVGSAGKIYTYGETYHASRRLAAGM 96

Query: 462  RKIGMAKGEVVMLLLQNCPEFVITFLGASMGGNVVTTANPFYTPADIAKQASASNTRIVV 641
              IG+ KG+V+M+LLQN  EFV +F+ ASM G V TTANPFYTPA+I KQ +AS  ++V+
Sbjct: 97   SAIGITKGDVIMILLQNSAEFVFSFIAASMIGAVATTANPFYTPAEIFKQITASKAKLVI 156

Query: 642  TSSSYVNKLGDLMSGGV----RVFTVDEPQEGCEHFSVLTDVADEGPLGNVSVDAEDTVA 809
            T + YV+KL D  S  +    +V TVD+P E C HFS +++  +E  L  V +  +D VA
Sbjct: 157  TQAMYVDKLRDSDSAKIGEDFKVVTVDDPPENCLHFSAISE-GNENDLPEVEIHPDDAVA 215

Query: 810  LPYSSGTTGLPKGVMLTHRSLVTSVAQQVDGDNPNLNLLPEDVVLCVLPLFHIYSLNSVL 989
            LP+SSGTTGLPKGV+LTHRSL TSVAQQVDG+NPNL L  EDV+LCVLPLFHI+SLNSVL
Sbjct: 216  LPFSSGTTGLPKGVVLTHRSLTTSVAQQVDGENPNLYLKTEDVLLCVLPLFHIFSLNSVL 275

Query: 990  LCSLRAGSAVLLMHKFEIGNLLEFVPKYKISXXXXXXXXXXXXXKNQMVESYDLSSVRFV 1169
            LC+LRAGSAVLLM KF+IG LLE + +Y++S             KN MV  YDLSS+R V
Sbjct: 276  LCALRAGSAVLLMQKFDIGTLLELIQRYRVSVAMVVPPLVLALAKNPMVVEYDLSSIRLV 335

Query: 1170 LSGAAPLGKELEEALRKRVPNAIFGQGYGMTEAGPVLSMCLGFAKEPTPMKFGSCGCVVR 1349
            LSGAAPLGKELEE LR RVP A+ GQGYGMTEAGPVLSMCLGFAK+P P K GSCG VVR
Sbjct: 336  LSGAAPLGKELEEGLRSRVPQAVLGQGYGMTEAGPVLSMCLGFAKQPFPTKSGSCGTVVR 395

Query: 1350 NAEMKIVDSDTGISLPRNKPGEICIRGAQIMKGYLNDPDSTARTIDEEGWLHTGDIGYVD 1529
            NAE+K+VD +TG SL  N+PGEICIRG QIMKGYLND  +TA TID +GWLHTGD+GYVD
Sbjct: 396  NAELKVVDPETGCSLGYNQPGEICIRGQQIMKGYLNDDTATATTIDVDGWLHTGDVGYVD 455

Query: 1530 DDDEVFIIDRVKEIIKYKGFQVPPAELEALLINHPSIADAAVVPLNDELAGEVPVAFLVK 1709
            DDDE+FI+DRVKE+IK+KGFQVPPAELEALL++HPSIADAAVVP  D  AGEVPVAF+V+
Sbjct: 456  DDDEIFIVDRVKELIKFKGFQVPPAELEALLVSHPSIADAAVVPQKDVAAGEVPVAFVVR 515

Query: 1710 SENGPGVTEEDIKQFVAKQVVYYKRLHKVNFIHAIPKSPSGKILRKDLKAKL 1865
            S NG  +TEE +K+F+AKQVV+YKRLHKV F+HAIPKSPSGKILRKDL+AKL
Sbjct: 516  S-NGFELTEESVKEFIAKQVVFYKRLHKVYFVHAIPKSPSGKILRKDLRAKL 566


>ALM23512.1 4-coumarate-CoA ligase 2 [Ziziphus jujuba]
          Length = 591

 Score =  717 bits (1852), Expect = 0.0
 Identities = 366/571 (64%), Positives = 444/571 (77%), Gaps = 8/571 (1%)
 Frame = +3

Query: 195  ATVPQITEPESPSVKVLEKKTEEIHKMEEYVFRSRLPDIYIPDNMSLHDYCFER-LHELR 371
            AT P    P  P    L ++ + IH     VF+S+LPDI I +++ LH YCFER L E  
Sbjct: 28   ATTPP--PPPPPPKNQLPQQDDTIH-----VFKSKLPDIPISNHVPLHTYCFERSLVEFP 80

Query: 372  DRPCLIQGSTGKILTYGEVEASSRRVAAGLRKIGMAKGEVVMLLLQNCPEFVITFLGASM 551
            DRPCLI GSTGK  T+ E    S ++AAGL  +G+ KG+V+M+LLQNC EFV  F+GAS+
Sbjct: 81   DRPCLIVGSTGKTYTFAETHLISSKIAAGLSTLGIKKGDVIMILLQNCAEFVFAFMGASI 140

Query: 552  GGNVVTTANPFYTPADIAKQASASNTRIVVTSSSYVNKLGDLMSG-------GVRVFTVD 710
             G V TTANPFYT  +I KQ +AS +++++T S YV+KL +   G         +V TVD
Sbjct: 141  IGAVSTTANPFYTVPEIFKQFNASKSKLIITQSQYVDKLREPGGGQYPKLGQDFKVITVD 200

Query: 711  EPQEGCEHFSVLTDVADEGPLGNVSVDAEDTVALPYSSGTTGLPKGVMLTHRSLVTSVAQ 890
            +P E C  FSV++D A+E     V +DAED VALP+SSGTTGLPKGV+LTH+SL+TSVAQ
Sbjct: 201  DPPENCLDFSVISD-ANENEFPEVVIDAEDPVALPFSSGTTGLPKGVVLTHKSLITSVAQ 259

Query: 891  QVDGDNPNLNLLPEDVVLCVLPLFHIYSLNSVLLCSLRAGSAVLLMHKFEIGNLLEFVPK 1070
            QVDGDNPNL L  EDVVLCVLP+FHI+SLNSVLL SLR G+AVL+MHKFEIG LLE + +
Sbjct: 260  QVDGDNPNLYLTEEDVVLCVLPMFHIFSLNSVLLNSLRVGAAVLIMHKFEIGALLELIQR 319

Query: 1071 YKISXXXXXXXXXXXXXKNQMVESYDLSSVRFVLSGAAPLGKELEEALRKRVPNAIFGQG 1250
            +++S             KN MV+ +DLSS+R VLSGAAPLGKELEEALR RVP A+ GQG
Sbjct: 320  HRVSVAAVVPPLVLALAKNPMVDKFDLSSIRLVLSGAAPLGKELEEALRNRVPQAVLGQG 379

Query: 1251 YGMTEAGPVLSMCLGFAKEPTPMKFGSCGCVVRNAEMKIVDSDTGISLPRNKPGEICIRG 1430
            YGMTEAGPVLSMCLGFAK+P P K GSCG VVRNAE+K+VD ++G SL  N+PGEICIRG
Sbjct: 380  YGMTEAGPVLSMCLGFAKQPFPTKSGSCGTVVRNAELKVVDPESGCSLGYNQPGEICIRG 439

Query: 1431 AQIMKGYLNDPDSTARTIDEEGWLHTGDIGYVDDDDEVFIIDRVKEIIKYKGFQVPPAEL 1610
            +QIMKGYLND ++TA TIDE+GWLHTGDIGYVDDDDE+FI+DRVKEIIK+KGFQVPPAEL
Sbjct: 440  SQIMKGYLNDGEATANTIDEDGWLHTGDIGYVDDDDEIFIVDRVKEIIKFKGFQVPPAEL 499

Query: 1611 EALLINHPSIADAAVVPLNDELAGEVPVAFLVKSENGPGVTEEDIKQFVAKQVVYYKRLH 1790
            EALL+ HPSIADAAVVP  DE+AGEVPVAF+V+S NG  +TEE +K+F++KQVV+YKRLH
Sbjct: 500  EALLVGHPSIADAAVVPQKDEVAGEVPVAFVVRSTNGVELTEEAVKEFISKQVVFYKRLH 559

Query: 1791 KVNFIHAIPKSPSGKILRKDLKAKLNEPIPN 1883
            KV F+HA+PKSPSG+ILRKDL+AKL    P+
Sbjct: 560  KVYFVHAVPKSPSGRILRKDLRAKLATASPS 590


>OAY41753.1 hypothetical protein MANES_09G127000 [Manihot esculenta]
          Length = 563

 Score =  716 bits (1849), Expect = 0.0
 Identities = 363/556 (65%), Positives = 433/556 (77%), Gaps = 6/556 (1%)
 Frame = +3

Query: 216  EPESPSVKVLEKKTEEIHKMEEYVFRSRLPDIYIPDNMSLHDYCFERLHELRDRPCLIQG 395
            EP  P V          H  + ++FRS+LPDI I +++SLH YCFE L    DRPCLI G
Sbjct: 10   EPSKPEVS-------PPHNSDAHIFRSKLPDIPISNHLSLHAYCFENLLSFADRPCLISG 62

Query: 396  STGKILTYGEVEASSRRVAAGLRKIGMAKGEVVMLLLQNCPEFVITFLGASMGGNVVTTA 575
            STGK  ++ E    S++ AAGL  +G+ KG+VVM+LLQNCPEFV +F+GASM G V TTA
Sbjct: 63   STGKTYSFAETHLVSQKCAAGLSNLGINKGDVVMILLQNCPEFVFSFMGASMIGAVTTTA 122

Query: 576  NPFYTPADIAKQASASNTRIVVTSSSYVNKLGDLMSGGVR------VFTVDEPQEGCEHF 737
            NPFYT  +I KQ ++S  ++++T S YV+KL D      +      V T+D+P E C HF
Sbjct: 123  NPFYTSNEIFKQFTSSRAKLIITQSQYVDKLRDSQENQPKLGQDFNVITIDDPPENCLHF 182

Query: 738  SVLTDVADEGPLGNVSVDAEDTVALPYSSGTTGLPKGVMLTHRSLVTSVAQQVDGDNPNL 917
            +VL++ A E  + +V++  +D VALP+SSGTTGLPKGV+LTH+SL+TSVAQQVDG+NPNL
Sbjct: 183  TVLSE-AKESEIPDVTIHPDDPVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNL 241

Query: 918  NLLPEDVVLCVLPLFHIYSLNSVLLCSLRAGSAVLLMHKFEIGNLLEFVPKYKISXXXXX 1097
             L  EDVVLCVLPLFHIYSLNSVLLCSLRAG+AVL M KFEIG LLE + K+K+S     
Sbjct: 242  YLKQEDVVLCVLPLFHIYSLNSVLLCSLRAGAAVLFMQKFEIGALLELIQKHKVSVAAVV 301

Query: 1098 XXXXXXXXKNQMVESYDLSSVRFVLSGAAPLGKELEEALRKRVPNAIFGQGYGMTEAGPV 1277
                    KN MV  +DLSS+R VLSGAAPLGKELE+ALR RVP AI GQGYGMTEAGPV
Sbjct: 302  PPLVLALAKNPMVAGFDLSSIRVVLSGAAPLGKELEDALRSRVPQAILGQGYGMTEAGPV 361

Query: 1278 LSMCLGFAKEPTPMKFGSCGCVVRNAEMKIVDSDTGISLPRNKPGEICIRGAQIMKGYLN 1457
            LSMCLGFAK+P P K GSCG VVRNAE+K++D +TG SL  N+PGEICIRG QIMKGYLN
Sbjct: 362  LSMCLGFAKQPFPTKSGSCGTVVRNAELKVIDPETGCSLGYNQPGEICIRGQQIMKGYLN 421

Query: 1458 DPDSTARTIDEEGWLHTGDIGYVDDDDEVFIIDRVKEIIKYKGFQVPPAELEALLINHPS 1637
            D ++TA TID EGWLHTGDIGYVDDD+EVFI+DRVKEIIK+KGFQVPPAELEALL+NHPS
Sbjct: 422  DLEATANTIDVEGWLHTGDIGYVDDDEEVFIVDRVKEIIKFKGFQVPPAELEALLVNHPS 481

Query: 1638 IADAAVVPLNDELAGEVPVAFLVKSENGPGVTEEDIKQFVAKQVVYYKRLHKVNFIHAIP 1817
            IADAAVVP  DE+AGE PVAF+V S NG  +TEE +K ++AKQVV+YK+LHKV F+HAIP
Sbjct: 482  IADAAVVPQKDEVAGEAPVAFVVPS-NGFELTEEAVKDYIAKQVVFYKKLHKVYFVHAIP 540

Query: 1818 KSPSGKILRKDLKAKL 1865
            KSPSGKILRKD+KAKL
Sbjct: 541  KSPSGKILRKDIKAKL 556


>BAG82851.1 4-coumarate:CoA ligase [Ipomoea batatas]
          Length = 569

 Score =  716 bits (1849), Expect = 0.0
 Identities = 359/562 (63%), Positives = 436/562 (77%), Gaps = 6/562 (1%)
 Frame = +3

Query: 216  EPESPSVKVLEKKTEEIHKMEEYVFRSRLPDIYIPDNMSLHDYCFERLHELRDRPCLIQG 395
            E + P ++V +    +    + ++FRSRLPDI IP+ + LH YCF+ L E RDR CLI G
Sbjct: 5    ETQKPELEVADSGKTQAQSSQIHIFRSRLPDIPIPNQIPLHTYCFQNLAEYRDRTCLIVG 64

Query: 396  STGKILTYGEVEASSRRVAAGLRKIGMAKGEVVMLLLQNCPEFVITFLGASMGGNVVTTA 575
            STGK  ++ E     R+VA+GL K+G+ KG+V+M L QNCPEFV +F+GASM G V TTA
Sbjct: 65   STGKTYSFAETHLICRKVASGLAKLGVKKGDVIMTLFQNCPEFVFSFMGASMIGAVTTTA 124

Query: 576  NPFYTPADIAKQASASNTRIVVTSSSYVNKLGDL------MSGGVRVFTVDEPQEGCEHF 737
            NPFYT A+I KQ SAS  ++V+T S YV+KL D       +     V T+D+P E C HF
Sbjct: 125  NPFYTKAEIFKQMSASKAKVVITQSVYVDKLKDSGEEYPKIGEDFSVVTIDDPPENCLHF 184

Query: 738  SVLTDVADEGPLGNVSVDAEDTVALPYSSGTTGLPKGVMLTHRSLVTSVAQQVDGDNPNL 917
            SVL++  +E     V +  +D VALP+SSGTTGLPKGV+LTH+SL+TSVAQQVDG+NPNL
Sbjct: 185  SVLSEADEEDMPATVEIAPDDAVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNL 244

Query: 918  NLLPEDVVLCVLPLFHIYSLNSVLLCSLRAGSAVLLMHKFEIGNLLEFVPKYKISXXXXX 1097
             L  +DVVLCVLPLFHI+SLNSVLLCSLRAG+AVLLM KFEI +LLE + K+++S     
Sbjct: 245  YLKEDDVVLCVLPLFHIFSLNSVLLCSLRAGAAVLLMQKFEIKSLLELIKKHRVSVAAVV 304

Query: 1098 XXXXXXXXKNQMVESYDLSSVRFVLSGAAPLGKELEEALRKRVPNAIFGQGYGMTEAGPV 1277
                    KN +V+SYDLSS+R VLSGAAPLGKELEEAL +RVP AIFGQGYGMTEAGPV
Sbjct: 305  PPLVLALAKNPIVDSYDLSSIRVVLSGAAPLGKELEEALHQRVPQAIFGQGYGMTEAGPV 364

Query: 1278 LSMCLGFAKEPTPMKFGSCGCVVRNAEMKIVDSDTGISLPRNKPGEICIRGAQIMKGYLN 1457
            LSMC  FAK+  P K GSCG VVRNAE+ +VD +TG SL RN+PGEICIRG+QIMK YLN
Sbjct: 365  LSMCPAFAKQALPAKSGSCGSVVRNAELMVVDPETGCSLGRNQPGEICIRGSQIMKEYLN 424

Query: 1458 DPDSTARTIDEEGWLHTGDIGYVDDDDEVFIIDRVKEIIKYKGFQVPPAELEALLINHPS 1637
            DP +TARTID +GWLHTGDIGYVDDDDEVFI+DRVKE+IK+KGFQVPPAELEALL++HP 
Sbjct: 425  DPAATARTIDVDGWLHTGDIGYVDDDDEVFIVDRVKELIKFKGFQVPPAELEALLLSHPM 484

Query: 1638 IADAAVVPLNDELAGEVPVAFLVKSENGPGVTEEDIKQFVAKQVVYYKRLHKVNFIHAIP 1817
            IADAAVVP  D+ AGEVPVAF+V+S +G  +TEE +K+F+AKQVV+YK+LHKV FIHAIP
Sbjct: 485  IADAAVVPQKDDAAGEVPVAFVVRSSDGFDLTEEAVKEFIAKQVVFYKKLHKVYFIHAIP 544

Query: 1818 KSPSGKILRKDLKAKLNEPIPN 1883
            KS SGKILRK+L+ KL    P+
Sbjct: 545  KSASGKILRKELREKLQAAPPS 566


>ADZ54779.1 4-coumarate-CoA ligase [Prunus avium]
          Length = 604

 Score =  717 bits (1852), Expect = 0.0
 Identities = 354/547 (64%), Positives = 434/547 (79%), Gaps = 14/547 (2%)
 Frame = +3

Query: 282  YVFRSRLPDIYIPDNMSLHDYCFERLHELRDRPCLIQGSTGKILTYGEVEASSRRVAAGL 461
            +VF+S+LPDI IP+++ LH YCF++L E  DRPCLI  STGK  ++ +    S++  AGL
Sbjct: 58   HVFKSKLPDIPIPNHLPLHTYCFQKLSEFSDRPCLIVSSTGKSYSFSDTYLISQKTGAGL 117

Query: 462  RKIGMAKGEVVMLLLQNCPEFVITFLGASMGGNVVTTANPFYTPADIAKQASASNTRIVV 641
              +G+ KG+V+M+LLQNC EFV  F+GASM G + TTANPFYT  +I KQ   SN ++++
Sbjct: 118  SNLGIKKGDVIMILLQNCAEFVFAFMGASMIGAITTTANPFYTATEIFKQFKNSNAKLII 177

Query: 642  TSSSYVNKLGDLMSGGV--------------RVFTVDEPQEGCEHFSVLTDVADEGPLGN 779
            T S +V+KL +  + G               +V TVD+P E C HFSV+++ A+E  L  
Sbjct: 178  THSQFVDKLREAPADGAAPDQNNYLKLGEHFKVVTVDDPPENCLHFSVISE-ANEKELPE 236

Query: 780  VSVDAEDTVALPYSSGTTGLPKGVMLTHRSLVTSVAQQVDGDNPNLNLLPEDVVLCVLPL 959
            V +D ED VALP+SSGTTGLPKGV+LTH+SL+TSVAQQVDG+NPNL L  +DVVLCVLPL
Sbjct: 237  VVIDPEDPVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLKEDDVVLCVLPL 296

Query: 960  FHIYSLNSVLLCSLRAGSAVLLMHKFEIGNLLEFVPKYKISXXXXXXXXXXXXXKNQMVE 1139
            FHIYSLNSV+LCSLRAG+AVL+MHKFEIG LLE + +Y++S             KN MV 
Sbjct: 297  FHIYSLNSVMLCSLRAGAAVLVMHKFEIGTLLELIQRYRVSVAAVVPPLVIALAKNPMVA 356

Query: 1140 SYDLSSVRFVLSGAAPLGKELEEALRKRVPNAIFGQGYGMTEAGPVLSMCLGFAKEPTPM 1319
             +DLSS+R VLSGAAPLGKELEEALR RVP A+ GQGYGMTEAGPVLSMCL FAKEP P 
Sbjct: 357  QFDLSSIRVVLSGAAPLGKELEEALRSRVPGAVLGQGYGMTEAGPVLSMCLAFAKEPLPS 416

Query: 1320 KFGSCGCVVRNAEMKIVDSDTGISLPRNKPGEICIRGAQIMKGYLNDPDSTARTIDEEGW 1499
            K GSCG V+RNAE+K++DS+TG SL  N+PGEICIRG+QIMKGYLND ++TA T+D+EGW
Sbjct: 417  KSGSCGTVIRNAELKVIDSETGCSLGYNQPGEICIRGSQIMKGYLNDAEATATTVDKEGW 476

Query: 1500 LHTGDIGYVDDDDEVFIIDRVKEIIKYKGFQVPPAELEALLINHPSIADAAVVPLNDELA 1679
            LHTGD+GYVDDD+EVFI+DRVKE+IK+KGFQVPPAELE+LL++HPSIADAAVVP  DE A
Sbjct: 477  LHTGDVGYVDDDEEVFIVDRVKELIKFKGFQVPPAELESLLVSHPSIADAAVVPQKDEAA 536

Query: 1680 GEVPVAFLVKSENGPGVTEEDIKQFVAKQVVYYKRLHKVNFIHAIPKSPSGKILRKDLKA 1859
            GEVPVAF+V+S NG  +TEE +K+F+AKQVV+YKRLHKV F+HAIPKSPSGKILRKDL+A
Sbjct: 537  GEVPVAFVVRS-NGLQLTEEAVKEFIAKQVVFYKRLHKVYFVHAIPKSPSGKILRKDLRA 595

Query: 1860 KLNEPIP 1880
            KL  P P
Sbjct: 596  KLATPTP 602