BLASTX nr result
ID: Ephedra28_contig00022137
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Ephedra28_contig00022137
(537 letters)
Database: ./nr
37,332,560 sequences; 13,225,080,153 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|ADX42738.1| (E)-beta-farnesene synthase 2 [Pseudotsuga menzie... 65 7e-09
gb|ADX42737.1| (E)-beta-farnesene synthase 1 [Pseudotsuga menzie... 65 7e-09
gb|AAX07265.1| (E)-beta-farnesene synthase [Pseudotsuga menziesii] 64 2e-08
gb|AGO02736.1| sabinene synthase [Thuja plicata] 64 2e-08
dbj|BAF43701.1| putative sesquiterpene synthase [Cycas taitungen... 63 5e-08
gb|ADZ45500.1| (-)-linalool synthase [Picea glauca] 62 6e-08
gb|ADZ45501.1| (-)-linalool synthase [Picea sitchensis] 62 1e-07
gb|ADH29869.1| e-beta farnesene synthase [Pinus sylvestris] 61 2e-07
gb|ACN41126.1| unknown [Picea sitchensis] 60 2e-07
gb|AFU73861.1| alpha terpineol /1,8-cineole synthase [Pinus cont... 60 4e-07
sp|F1CKI8.1|3CAR2_PICSI RecName: Full=Carene synthase 2, chlorop... 59 5e-07
gb|AFU73860.1| alpha terpineol synthase [Pinus banksiana] 59 7e-07
sp|Q9M7D1.1|TPSD8_ABIGR RecName: Full=Beta-phellandrene synthase... 59 9e-07
gb|AAC24191.1| E-alpha-bisabolene synthase [Abies grandis] 58 1e-06
sp|O81086.1|TPSD1_ABIGR RecName: Full=Alpha-bisabolene synthase;... 58 1e-06
gb|ADZ45514.1| (E,E)-alpha-farnesene synthase/(E)-beta-ocimene s... 57 2e-06
gb|AAS47697.1| E,E-alpha-farnesene synthase [Picea abies] 57 2e-06
sp|Q84KL5.1|PT5_PINTA RecName: Full=Alpha-farnesene synthase gi|... 57 3e-06
gb|AFJ73578.1| 2-methyl-3-buten-2-ol synthase [Pinus teocote] 57 3e-06
gb|AFJ73577.1| 2-methyl-3-buten-2-ol synthase [Pinus teocote] 57 3e-06
>gb|ADX42738.1| (E)-beta-farnesene synthase 2 [Pseudotsuga menziesii]
gi|323364474|gb|ADX42739.1| (E)-beta-farnesene synthase 2
[Pseudotsuga menziesii]
Length = 825
Score = 65.5 bits (158), Expect = 7e-09
Identities = 33/112 (29%), Positives = 57/112 (50%), Gaps = 1/112 (0%)
Frame = -2
Query: 476 RILDEMVDY-LEYPQGEAVKSVECLLKENSGYSSEDALCFLHEMVRESVKHIAKLYFSPE 300
R+ D++ Y E +GE S+EC +KE+ G + E A+ +++ ++ +VK + + PE
Sbjct: 714 RLADDIHTYKAEKARGELASSIECYMKEHPGSTEEVAVNYMYSLLEPAVKELTWEFLKPE 773
Query: 299 YDTEEEPLMLKRIAFEVARATYLLLNEGDGYSKEPQIFKDLVALVFFNPVPL 144
T P K++ E R T ++ EGDG+ KD + P+PL
Sbjct: 774 DSTVHIPFQCKKMLMEETRVTMVIFKEGDGFGISKTKIKDYIKDCLIEPLPL 825
>gb|ADX42737.1| (E)-beta-farnesene synthase 1 [Pseudotsuga menziesii]
Length = 825
Score = 65.5 bits (158), Expect = 7e-09
Identities = 33/112 (29%), Positives = 57/112 (50%), Gaps = 1/112 (0%)
Frame = -2
Query: 476 RILDEMVDY-LEYPQGEAVKSVECLLKENSGYSSEDALCFLHEMVRESVKHIAKLYFSPE 300
R+ D++ Y E +GE S+EC +KE+ G + E A+ +++ ++ +VK + + PE
Sbjct: 714 RLADDIHTYKAEKARGELASSIECYMKEHPGSTEEVAVNYMYSLLEPAVKELTWEFLKPE 773
Query: 299 YDTEEEPLMLKRIAFEVARATYLLLNEGDGYSKEPQIFKDLVALVFFNPVPL 144
T P K++ E R T ++ EGDG+ KD + P+PL
Sbjct: 774 DSTVHIPFQCKKMLMEETRVTMVIFKEGDGFGISKTKIKDYIKDCLIEPLPL 825
>gb|AAX07265.1| (E)-beta-farnesene synthase [Pseudotsuga menziesii]
Length = 826
Score = 64.3 bits (155), Expect = 2e-08
Identities = 32/112 (28%), Positives = 57/112 (50%), Gaps = 1/112 (0%)
Frame = -2
Query: 476 RILDEMVDY-LEYPQGEAVKSVECLLKENSGYSSEDALCFLHEMVRESVKHIAKLYFSPE 300
R+ D++ Y E +GE S+EC ++E+ G + E A+ +++ ++ +VK + + PE
Sbjct: 715 RLADDIHTYKAEKARGELASSIECYMREHPGSTEEVAVNYMYSLLEPAVKELTWEFLKPE 774
Query: 299 YDTEEEPLMLKRIAFEVARATYLLLNEGDGYSKEPQIFKDLVALVFFNPVPL 144
T P K++ E R T ++ EGDG+ KD + P+PL
Sbjct: 775 DSTVHIPFQCKKMLMEETRVTMVIFKEGDGFGISKTKIKDYIKDCLIEPLPL 826
>gb|AGO02736.1| sabinene synthase [Thuja plicata]
Length = 604
Score = 63.9 bits (154), Expect = 2e-08
Identities = 32/101 (31%), Positives = 56/101 (55%)
Frame = -2
Query: 446 EYPQGEAVKSVECLLKENSGYSSEDALCFLHEMVRESVKHIAKLYFSPEYDTEEEPLMLK 267
E +GE V + C +K++ G S E+AL +L +++++ +K + + Y P P + K
Sbjct: 508 EANRGELVSGISCYIKDHPGSSEEEALDYLKDLLQKRLKELDQEYLKP----NNVPAISK 563
Query: 266 RIAFEVARATYLLLNEGDGYSKEPQIFKDLVALVFFNPVPL 144
A+ +AR+ LL E DG++ + KDLV + P+PL
Sbjct: 564 DHAYNIARSYQLLYKERDGFTNSNKDIKDLVTQILLEPIPL 604
>dbj|BAF43701.1| putative sesquiterpene synthase [Cycas taitungensis]
Length = 846
Score = 62.8 bits (151), Expect = 5e-08
Identities = 33/101 (32%), Positives = 50/101 (49%)
Frame = -2
Query: 446 EYPQGEAVKSVECLLKENSGYSSEDALCFLHEMVRESVKHIAKLYFSPEYDTEEEPLMLK 267
E +GE S+EC +KENSG + +DAL + +VK + Y P ++ P K
Sbjct: 750 EKARGELASSIECYMKENSGSTQDDALNHIRAATSSAVKEMTWEYLKP----DDVPYRCK 805
Query: 266 RIAFEVARATYLLLNEGDGYSKEPQIFKDLVALVFFNPVPL 144
+ FEVAR L+ +GD Y + +D + PVP+
Sbjct: 806 KFVFEVARVWMLMYKDGDPYGISNKEMQDYIREALIEPVPI 846
>gb|ADZ45500.1| (-)-linalool synthase [Picea glauca]
Length = 627
Score = 62.4 bits (150), Expect = 6e-08
Identities = 34/113 (30%), Positives = 60/113 (53%), Gaps = 1/113 (0%)
Frame = -2
Query: 479 MRILDEMVDY-LEYPQGEAVKSVECLLKENSGYSSEDALCFLHEMVRESVKHIAKLYFSP 303
+R+ +++ Y E +GE + C +K+N G + EDA+ +++ MV +S+K + + P
Sbjct: 518 LRLKGDILTYQAERSRGEKSSCISCYMKDNPGSTEEDAVAYINGMVNKSLKELNWEFLRP 577
Query: 302 EYDTEEEPLMLKRIAFEVARATYLLLNEGDGYSKEPQIFKDLVALVFFNPVPL 144
+ P+ K+ AF++ RA Y L DG+S ++LV PVPL
Sbjct: 578 D---SNAPITSKKHAFDILRAFYHLYKHRDGFSVARNEIRNLVKTTVIEPVPL 627
>gb|ADZ45501.1| (-)-linalool synthase [Picea sitchensis]
Length = 627
Score = 61.6 bits (148), Expect = 1e-07
Identities = 36/123 (29%), Positives = 63/123 (51%), Gaps = 1/123 (0%)
Frame = -2
Query: 509 STLLKECSRTMRILDEMVDY-LEYPQGEAVKSVECLLKENSGYSSEDALCFLHEMVRESV 333
S+L + +R+ ++ Y E +GE + C +K+N G + EDA+ +++ MV +S+
Sbjct: 508 SSLNRLACSILRLKGDIHTYQAERSRGEKSSCISCYMKDNPGSTEEDAVTYINAMVNKSL 567
Query: 332 KHIAKLYFSPEYDTEEEPLMLKRIAFEVARATYLLLNEGDGYSKEPQIFKDLVALVFFNP 153
K + + P+ P+ K+ AF++ RA Y L DG+S ++LV P
Sbjct: 568 KELNWEFLRPD---SNAPITSKKHAFDILRAFYHLYKHRDGFSVARNEIRNLVKTTVIEP 624
Query: 152 VPL 144
VPL
Sbjct: 625 VPL 627
>gb|ADH29869.1| e-beta farnesene synthase [Pinus sylvestris]
Length = 811
Score = 60.8 bits (146), Expect = 2e-07
Identities = 31/113 (27%), Positives = 59/113 (52%), Gaps = 2/113 (1%)
Frame = -2
Query: 476 RILDEMVDY-LEYPQGEAVKSVECLLKENSGYSSEDALCFLHEMVRESVKHIAKLYFSPE 300
R+ D++ Y E +GE S+EC +KE+ G + E A+ +++ ++ +VK + + P+
Sbjct: 699 RLADDIHTYKAEKARGELASSIECYMKEHPGSTEEVAVNYMYSLLEPAVKELTWEFLKPD 758
Query: 299 YDTEEE-PLMLKRIAFEVARATYLLLNEGDGYSKEPQIFKDLVALVFFNPVPL 144
++ + P K++ E R T ++ EGDG+ KD + P+PL
Sbjct: 759 DTSDVDIPFQCKKMLMEETRVTMVIFKEGDGFGISKTKIKDYIKECLIEPLPL 811
>gb|ACN41126.1| unknown [Picea sitchensis]
Length = 627
Score = 60.5 bits (145), Expect = 2e-07
Identities = 33/113 (29%), Positives = 60/113 (53%), Gaps = 1/113 (0%)
Frame = -2
Query: 479 MRILDEMVDY-LEYPQGEAVKSVECLLKENSGYSSEDALCFLHEMVRESVKHIAKLYFSP 303
+R+ +++ Y E +GE + C +K+N G + E+A+ +++ MV +S+K + + P
Sbjct: 518 LRLKGDILTYQAERSRGEKSSCISCYMKDNPGSTEENAVAYINGMVNKSLKELNWEFLRP 577
Query: 302 EYDTEEEPLMLKRIAFEVARATYLLLNEGDGYSKEPQIFKDLVALVFFNPVPL 144
+ P+ K+ AF++ RA Y L DG+S ++LV PVPL
Sbjct: 578 D---SNAPITSKKHAFDILRAFYHLYKHRDGFSVARNEIRNLVKTTVIEPVPL 627
>gb|AFU73861.1| alpha terpineol /1,8-cineole synthase [Pinus contorta]
Length = 616
Score = 59.7 bits (143), Expect = 4e-07
Identities = 32/98 (32%), Positives = 49/98 (50%)
Frame = -2
Query: 437 QGEAVKSVECLLKENSGYSSEDALCFLHEMVRESVKHIAKLYFSPEYDTEEEPLMLKRIA 258
+GE + C +KEN G + ED + +H MV + +K + P+ + P+ K+ A
Sbjct: 522 RGEEASCISCYMKENPGLTEEDVVNHIHGMVDDLIKELNWELLKPDCNV---PISSKKHA 578
Query: 257 FEVARATYLLLNEGDGYSKEPQIFKDLVALVFFNPVPL 144
F++ RA + DGYS KDLV + PVPL
Sbjct: 579 FDICRAVHHGYKYRDGYSVATNEIKDLVMITVLEPVPL 616
>sp|F1CKI8.1|3CAR2_PICSI RecName: Full=Carene synthase 2, chloroplastic; Short=PsTPS-3car2;
AltName: Full=(+)-car-3-ene synthase 2; AltName:
Full=3-carene cyclase 2; Flags: Precursor
gi|317016740|gb|ADU85926.1| (+)-3-carene synthase [Picea
sitchensis]
Length = 627
Score = 59.3 bits (142), Expect = 5e-07
Identities = 29/98 (29%), Positives = 52/98 (53%)
Frame = -2
Query: 437 QGEAVKSVECLLKENSGYSSEDALCFLHEMVRESVKHIAKLYFSPEYDTEEEPLMLKRIA 258
+GE + C +K+N G + EDAL ++ M+ E++K + + P+ + P+ K+ A
Sbjct: 533 RGEEASCISCYMKDNPGSTQEDALNHINNMIEETIKKLNRELLKPDNNV---PISSKKHA 589
Query: 257 FEVARATYLLLNEGDGYSKEPQIFKDLVALVFFNPVPL 144
F+++R + N DGY+ K+LV PVP+
Sbjct: 590 FDISRGLHHFYNYRDGYTVASNETKNLVIKTVLEPVPM 627
>gb|AFU73860.1| alpha terpineol synthase [Pinus banksiana]
Length = 626
Score = 58.9 bits (141), Expect = 7e-07
Identities = 32/98 (32%), Positives = 49/98 (50%)
Frame = -2
Query: 437 QGEAVKSVECLLKENSGYSSEDALCFLHEMVRESVKHIAKLYFSPEYDTEEEPLMLKRIA 258
+GE + C +KEN G + ED + +H MV + +K + P+ + P+ K+ A
Sbjct: 532 RGEEASCISCYMKENPGLTEEDVVNHIHGMVDDLIKELNWELLKPDCNV---PISSKKHA 588
Query: 257 FEVARATYLLLNEGDGYSKEPQIFKDLVALVFFNPVPL 144
F++ RA + DGYS KDLV + PVPL
Sbjct: 589 FDICRAFHHGYKYRDGYSVATNEIKDLVMITVLEPVPL 626
>sp|Q9M7D1.1|TPSD8_ABIGR RecName: Full=Beta-phellandrene synthase, chloroplastic; AltName:
Full=(-)-(4S)-beta-phellandrene synthase; AltName:
Full=Agg-Bphe; Flags: Precursor
gi|7381249|gb|AAF61453.1|AF139205_1 beta-phellandrene
synthase [Abies grandis]
Length = 630
Score = 58.5 bits (140), Expect = 9e-07
Identities = 31/98 (31%), Positives = 51/98 (52%)
Frame = -2
Query: 437 QGEAVKSVECLLKENSGYSSEDALCFLHEMVRESVKHIAKLYFSPEYDTEEEPLMLKRIA 258
+GE S+ C +K+N G + EDAL ++ M+ + +K + P+ + P+ ++ A
Sbjct: 536 RGEEASSISCYMKDNPGLTEEDALNHINAMINDIIKELNWELLKPDSNI---PMTARKHA 592
Query: 257 FEVARATYLLLNEGDGYSKEPQIFKDLVALVFFNPVPL 144
+E+ RA + L DG+S Q K LV PVPL
Sbjct: 593 YEITRAFHQLYKYRDGFSVATQETKSLVRRTVLEPVPL 630
>gb|AAC24191.1| E-alpha-bisabolene synthase [Abies grandis]
Length = 782
Score = 58.2 bits (139), Expect = 1e-06
Identities = 30/112 (26%), Positives = 57/112 (50%), Gaps = 1/112 (0%)
Frame = -2
Query: 476 RILDEMVDY-LEYPQGEAVKSVECLLKENSGYSSEDALCFLHEMVRESVKHIAKLYFSPE 300
R+ D+ Y E +GE S+EC +K++ + E+AL ++ ++ +VK + + + P
Sbjct: 675 RLADDTKTYKAEKARGELASSIECYMKDHPECTEEEALDHIYSILEPAVKELTREFLKP- 733
Query: 299 YDTEEEPLMLKRIAFEVARATYLLLNEGDGYSKEPQIFKDLVALVFFNPVPL 144
++ P K++ FE R T ++ +GDG+ KD + P+PL
Sbjct: 734 ---DDVPFACKKMLFEETRVTMVIFKDGDGFGVSKLEVKDHIKECLIEPLPL 782
>sp|O81086.1|TPSD1_ABIGR RecName: Full=Alpha-bisabolene synthase; AltName:
Full=(E)-alpha-bisabolene synthase; AltName:
Full=AgfEabis gi|361131501|pdb|3SAE|A Chain A, Structure
Of A Three-Domain Sesquiterpene Synthase: A Prospective
Target For Advanced Biofuels Production
gi|361131502|pdb|3SDQ|A Chain A, Structure Of A
Three-Domain Sesquiterpene Synthase: A Prospective Target
For Advanced Biofuels Production gi|361131554|pdb|3SDR|A
Chain A, Structure Of A Three-Domain Sesquiterpene
Synthase: A Prospective Target For Advanced Biofuels
Production gi|361131555|pdb|3SDT|A Chain A, Structure Of
A Three-Domain Sesquiterpene Synthase: A Prospective
Target For Advanced Biofuels Production
gi|361131556|pdb|3SDU|A Chain A, Structure Of A
Three-Domain Sesquiterpene Synthase: A Prospective Target
For Advanced Biofuels Production gi|361131583|pdb|3SDV|A
Chain A, Structure Of A Three-Domain Sesquiterpene
Synthase: A Prospective Target For Advanced Biofuels
Production gi|3252840|gb|AAC24192.1| E-alpha-bisabolene
synthase [Abies grandis]
Length = 817
Score = 58.2 bits (139), Expect = 1e-06
Identities = 30/112 (26%), Positives = 57/112 (50%), Gaps = 1/112 (0%)
Frame = -2
Query: 476 RILDEMVDY-LEYPQGEAVKSVECLLKENSGYSSEDALCFLHEMVRESVKHIAKLYFSPE 300
R+ D+ Y E +GE S+EC +K++ + E+AL ++ ++ +VK + + + P
Sbjct: 710 RLADDTKTYKAEKARGELASSIECYMKDHPECTEEEALDHIYSILEPAVKELTREFLKP- 768
Query: 299 YDTEEEPLMLKRIAFEVARATYLLLNEGDGYSKEPQIFKDLVALVFFNPVPL 144
++ P K++ FE R T ++ +GDG+ KD + P+PL
Sbjct: 769 ---DDVPFACKKMLFEETRVTMVIFKDGDGFGVSKLEVKDHIKECLIEPLPL 817
>gb|ADZ45514.1| (E,E)-alpha-farnesene synthase/(E)-beta-ocimene synthase [Picea
engelmannii x Picea glauca]
Length = 580
Score = 57.4 bits (137), Expect = 2e-06
Identities = 34/112 (30%), Positives = 56/112 (50%), Gaps = 1/112 (0%)
Frame = -2
Query: 479 MRILDEMVDY-LEYPQGEAVKSVECLLKENSGYSSEDALCFLHEMVRESVKHIAKLYFSP 303
+R+ ++ Y E +GE S+ C +K+N G + EDAL + M++E +K + + P
Sbjct: 471 LRLRGDVCGYKAERSRGEEASSISCYMKDNPGATEEDALNQIDTMIKEKIKELNWEFLKP 530
Query: 302 EYDTEEEPLMLKRIAFEVARATYLLLNEGDGYSKEPQIFKDLVALVFFNPVP 147
+ + P+ K+ AF++ RA Y L DG+S K LV VP
Sbjct: 531 DSNV---PISSKKHAFDILRAFYHLYKYRDGFSIANNETKSLVMRTLLETVP 579
>gb|AAS47697.1| E,E-alpha-farnesene synthase [Picea abies]
Length = 580
Score = 57.4 bits (137), Expect = 2e-06
Identities = 34/116 (29%), Positives = 58/116 (50%), Gaps = 1/116 (0%)
Frame = -2
Query: 488 SRTMRILDEMVDY-LEYPQGEAVKSVECLLKENSGYSSEDALCFLHEMVRESVKHIAKLY 312
S +R+ ++ Y E +GE S+ C +K+N G + EDAL ++ M+ +++ +
Sbjct: 468 SSILRLRGDICGYQAERSRGEEASSISCYMKDNPGSTEEDALSHINAMISDNINELNWEL 527
Query: 311 FSPEYDTEEEPLMLKRIAFEVARATYLLLNEGDGYSKEPQIFKDLVALVFFNPVPL 144
P + P+ K+ AF++ RA Y L DG+S K+LV PVP+
Sbjct: 528 LKPNSNV---PISSKKHAFDILRAFYHLYKYRDGFSIAKIETKNLVMRTVLEPVPM 580
>sp|Q84KL5.1|PT5_PINTA RecName: Full=Alpha-farnesene synthase gi|28894484|gb|AAO61226.1|
alpha-farnesene synthase [Pinus taeda]
Length = 574
Score = 57.0 bits (136), Expect = 3e-06
Identities = 34/116 (29%), Positives = 57/116 (49%), Gaps = 1/116 (0%)
Frame = -2
Query: 488 SRTMRILDEMVDY-LEYPQGEAVKSVECLLKENSGYSSEDALCFLHEMVRESVKHIAKLY 312
S +R+ ++ Y E +GE S+ C +K+N G + EDAL ++ M+ + + +
Sbjct: 463 SSILRLRGDICGYQAERSRGEQASSISCYMKDNPGSTEEDALSHVNAMIGDKIPEFNWEF 522
Query: 311 FSPEYDTEEEPLMLKRIAFEVARATYLLLNEGDGYSKEPQIFKDLVALVFFNPVPL 144
P + P+ K+ AF++ RA Y L DG+S K LV +PVP+
Sbjct: 523 MKP----SKAPISSKKYAFDILRAFYHLYKYRDGFSIAKIETKKLVMRTVLDPVPM 574
>gb|AFJ73578.1| 2-methyl-3-buten-2-ol synthase [Pinus teocote]
Length = 614
Score = 56.6 bits (135), Expect = 3e-06
Identities = 32/101 (31%), Positives = 52/101 (51%)
Frame = -2
Query: 446 EYPQGEAVKSVECLLKENSGYSSEDALCFLHEMVRESVKHIAKLYFSPEYDTEEEPLMLK 267
E +GE + C ++EN + EDA+ ++ MV + +K + + Y P+ + P+ K
Sbjct: 517 ERSRGEESSCISCYMEENPESTEEDAIDHINSMVDKLLKELNREYLRPDSNV---PITSK 573
Query: 266 RIAFEVARATYLLLNEGDGYSKEPQIFKDLVALVFFNPVPL 144
+ AF++ RA Y L DG+S K+LV PVPL
Sbjct: 574 KHAFDILRAFYHLYKYRDGFSVANYEIKNLVMTTVIEPVPL 614
>gb|AFJ73577.1| 2-methyl-3-buten-2-ol synthase [Pinus teocote]
Length = 614
Score = 56.6 bits (135), Expect = 3e-06
Identities = 32/101 (31%), Positives = 52/101 (51%)
Frame = -2
Query: 446 EYPQGEAVKSVECLLKENSGYSSEDALCFLHEMVRESVKHIAKLYFSPEYDTEEEPLMLK 267
E +GE + C ++EN + EDA+ ++ MV + +K + + Y P+ + P+ K
Sbjct: 517 ERSRGEESSCISCYMEENPESTEEDAIDHINSMVDKLLKELNREYLRPDSNV---PITSK 573
Query: 266 RIAFEVARATYLLLNEGDGYSKEPQIFKDLVALVFFNPVPL 144
+ AF++ RA Y L DG+S K+LV PVPL
Sbjct: 574 KHAFDILRAFYHLYKYRDGFSVANYEIKNLVMTTVIEPVPL 614