BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Ephedra28_contig00020686
         (777 letters)

Database: ./nr 
           37,332,560 sequences; 13,225,080,153 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ABR18163.1| unknown [Picea sitchensis]                             377   e-102
ref|XP_002991365.1| hypothetical protein SELMODRAFT_236271 [Sela...   343   3e-92
ref|XP_002988231.1| hypothetical protein SELMODRAFT_235490 [Sela...   341   1e-91
gb|AAT85663.1| polyunsaturated fatty acid delta-5-desaturase [Ma...   334   2e-89
ref|XP_001766264.1| predicted protein [Physcomitrella patens] gi...   318   2e-84
gb|ADB81956.1| delta 5 desaturase [Lobosphaera incisa] gi|358251...   285   1e-74
ref|XP_005650943.1| acyl-CoA dependent delta5-desaturase [Coccom...   268   1e-69
ref|XP_001420855.1| predicted protein [Ostreococcus lucimarinus ...   268   2e-69
ref|XP_003055412.1| predicted protein [Micromonas pusilla CCMP15...   268   2e-69
ref|XP_003082424.1| polyunsaturated fatty acid delta-5-desaturas...   265   1e-68
emb|CAQ30478.1| Acyl-CoA dependent delta5-desaturase [Mantoniell...   260   3e-67
ref|XP_002507174.1| predicted protein [Micromonas sp. RCC299] gi...   255   1e-65
emb|CCO66066.1| predicted protein [Bathycoccus prasinos]              251   1e-64
gb|ADM78345.1| sphingolipid desaturase-like protein, partial [Pi...   246   6e-63
gb|ADM78344.1| sphingolipid desaturase-like protein, partial [Pi...   246   6e-63
gb|ADM78346.1| sphingolipid desaturase-like protein, partial [Pi...   246   8e-63
gb|ADM78388.1| sphingolipid desaturase-like protein, partial [Pi...   245   1e-62
gb|ADM78374.1| sphingolipid desaturase-like protein, partial [Pi...   244   2e-62
ref|XP_002682732.1| polyunsaturated fatty acid delta-5-desaturas...   190   4e-46
ref|XP_003292036.1| hypothetical protein DICPUDRAFT_39933 [Dicty...   188   2e-45

>gb|ABR18163.1| unknown [Picea sitchensis]
          Length = 472

 Score =  377 bits (967), Expect = e-102
 Identities = 171/258 (66%), Positives = 206/258 (79%)
 Frame = +3

Query: 3   GASSFMWRHQHVVGHHSYTNVDNYDPDIRVKDPDVRRVTAKQPIRKYHAFQHVYXXXXXX 182
           GASSFMWR QHVVGHHS+TNV+NYDPDIRVKDPDVRRVT +QPI+KYH+FQH+Y      
Sbjct: 214 GASSFMWRQQHVVGHHSFTNVENYDPDIRVKDPDVRRVTYEQPIQKYHSFQHLYLGALYG 273

Query: 183 XXXXXXXXXDDFVAFFQGSIGPVKIPKMTVSEAAVFFGGKIFYAIYMILVPLLYSHHSMS 362
                    DDFVAF  GSIGPVKIP+MT  E  VF GGKI Y +YM ++P  +SHH+++
Sbjct: 274 LLALKGVLLDDFVAFITGSIGPVKIPEMTTLEFGVFIGGKILYTLYMFVLPSFFSHHNVA 333

Query: 363 SCIILYVVSQLIAGWVLAFLFQVAHVVSEVEFLAVDTSEGVPKLNKGWAEAQVRSTANFS 542
             +ILY+VSQL+ GW LAFLFQ+AHVV E  F  VD+S+G PKL++GWA  QVR+T NFS
Sbjct: 334 LRVILYIVSQLVCGWTLAFLFQLAHVVPEAAFPVVDSSDGRPKLHQGWAAMQVRTTTNFS 393

Query: 543 TNSTIWTHISGGLNFQIEHHLFPSFCHLHYPMIHSIVRETCREFNVPYFSYPSFWAALLA 722
            NS +WTH+SGGLN+QIEHHLFPS CHL+YP IHSIV+ETC++F+VPY SYPSFW AL A
Sbjct: 394 ANSMLWTHLSGGLNYQIEHHLFPSVCHLYYPSIHSIVKETCKDFDVPYHSYPSFWTALKA 453

Query: 723 HFQHLKRVGTENFVLRVS 776
           HF HLK+VG++NF L +S
Sbjct: 454 HFLHLKKVGSDNFELHLS 471


>ref|XP_002991365.1| hypothetical protein SELMODRAFT_236271 [Selaginella moellendorffii]
           gi|300140758|gb|EFJ07477.1| hypothetical protein
           SELMODRAFT_236271 [Selaginella moellendorffii]
          Length = 453

 Score =  343 bits (881), Expect = 3e-92
 Identities = 155/249 (62%), Positives = 185/249 (74%)
 Frame = +3

Query: 3   GASSFMWRHQHVVGHHSYTNVDNYDPDIRVKDPDVRRVTAKQPIRKYHAFQHVYXXXXXX 182
           GASSFMW++QHVVGHHS+TNV NYDPDIRVKDPD+RRVT+KQP   YH +QHVY      
Sbjct: 196 GASSFMWKYQHVVGHHSFTNVHNYDPDIRVKDPDIRRVTSKQPWHDYHMYQHVYLAILYG 255

Query: 183 XXXXXXXXXDDFVAFFQGSIGPVKIPKMTVSEAAVFFGGKIFYAIYMILVPLLYSHHSMS 362
                    DDFVA+F G IGPVK+PKMT  E  +F+ GK  YA YM+++PLL+SHH+  
Sbjct: 256 LLALKSIFLDDFVAYFNGCIGPVKVPKMTPVEFHIFWWGKAVYATYMLVLPLLFSHHNFL 315

Query: 363 SCIILYVVSQLIAGWVLAFLFQVAHVVSEVEFLAVDTSEGVPKLNKGWAEAQVRSTANFS 542
           S ++ Y+VSQL+ GW+LA LFQVAHVV E EF  VD + GVP L+ GWA  QVR+TANFS
Sbjct: 316 SLLVCYLVSQLVTGWMLALLFQVAHVVEEAEFFDVDCTNGVPTLSSGWAALQVRTTANFS 375

Query: 543 TNSTIWTHISGGLNFQIEHHLFPSFCHLHYPMIHSIVRETCREFNVPYFSYPSFWAALLA 722
            NS  WTH+SGGLN QIEHHLFP+ CHLHYP I  IV  TC+EF +PY  +P  W AL A
Sbjct: 376 VNSLFWTHVSGGLNHQIEHHLFPAICHLHYPRIQPIVESTCKEFGIPYHRFPDVWTALKA 435

Query: 723 HFQHLKRVG 749
           HF +LK+VG
Sbjct: 436 HFAYLKKVG 444


>ref|XP_002988231.1| hypothetical protein SELMODRAFT_235490 [Selaginella moellendorffii]
           gi|300143963|gb|EFJ10650.1| hypothetical protein
           SELMODRAFT_235490 [Selaginella moellendorffii]
          Length = 453

 Score =  341 bits (875), Expect = 1e-91
 Identities = 154/249 (61%), Positives = 185/249 (74%)
 Frame = +3

Query: 3   GASSFMWRHQHVVGHHSYTNVDNYDPDIRVKDPDVRRVTAKQPIRKYHAFQHVYXXXXXX 182
           GASSFMW++QHVVGHHS+TNV NYDPDIRVKDPD+RRVT+KQP   YH +QHVY      
Sbjct: 196 GASSFMWKYQHVVGHHSFTNVHNYDPDIRVKDPDIRRVTSKQPWHDYHMYQHVYLAILYG 255

Query: 183 XXXXXXXXXDDFVAFFQGSIGPVKIPKMTVSEAAVFFGGKIFYAIYMILVPLLYSHHSMS 362
                    DDFVA+F G IGPVK+PKMT  E  +F+ GK  YA YM+++PLL+S+H+  
Sbjct: 256 LLALKSIFLDDFVAYFNGRIGPVKVPKMTPVEFHIFWWGKAVYATYMLVLPLLFSYHNFL 315

Query: 363 SCIILYVVSQLIAGWVLAFLFQVAHVVSEVEFLAVDTSEGVPKLNKGWAEAQVRSTANFS 542
           S ++ Y+VSQL+ GW+LA LFQVAHVV E EF  VD + GVP L+ GWA  QVR+TANFS
Sbjct: 316 SLLVCYLVSQLVTGWMLALLFQVAHVVEEAEFFDVDCTNGVPTLSSGWAALQVRTTANFS 375

Query: 543 TNSTIWTHISGGLNFQIEHHLFPSFCHLHYPMIHSIVRETCREFNVPYFSYPSFWAALLA 722
            NS  WTH+SGGLN QIEHHLFP+ CHLHYP I  IV  TC+EF +PY  +P  W AL A
Sbjct: 376 VNSLFWTHVSGGLNHQIEHHLFPAICHLHYPRIQPIVESTCKEFGIPYHRFPDVWTALKA 435

Query: 723 HFQHLKRVG 749
           HF +LK+VG
Sbjct: 436 HFAYLKKVG 444


>gb|AAT85663.1| polyunsaturated fatty acid delta-5-desaturase [Marchantia
           polymorpha]
          Length = 484

 Score =  334 bits (856), Expect = 2e-89
 Identities = 152/252 (60%), Positives = 186/252 (73%)
 Frame = +3

Query: 3   GASSFMWRHQHVVGHHSYTNVDNYDPDIRVKDPDVRRVTAKQPIRKYHAFQHVYXXXXXX 182
           GASSFMWR QHV GHHS+TN+D+YDPDIRVKDPD+RRVT++QP R +H +QH+Y      
Sbjct: 227 GASSFMWRQQHVAGHHSFTNIDHYDPDIRVKDPDLRRVTSQQPRRWFHEYQHIYLGVLYG 286

Query: 183 XXXXXXXXXDDFVAFFQGSIGPVKIPKMTVSEAAVFFGGKIFYAIYMILVPLLYSHHSMS 362
                    DDF AFF G+IGPVKI +MT  E  VF+GGK+ YA+YM L+P++Y  +++ 
Sbjct: 287 VLALKSVLIDDFSAFFSGAIGPVKIAQMTPLEMGVFWGGKVVYALYMFLLPMMYGQYNIL 346

Query: 363 SCIILYVVSQLIAGWVLAFLFQVAHVVSEVEFLAVDTSEGVPKLNKGWAEAQVRSTANFS 542
           + I LY++SQL+AGW LA  FQVAHVV +  F   +T  G  K+  GWAE QVR+T NFS
Sbjct: 347 TFIGLYILSQLVAGWTLALFFQVAHVVDDAVFPVAETDGGKAKIPSGWAEMQVRTTTNFS 406

Query: 543 TNSTIWTHISGGLNFQIEHHLFPSFCHLHYPMIHSIVRETCREFNVPYFSYPSFWAALLA 722
           + S  WTHISGGLN QIEHHLFP  CH+HYP I  IV+ TC EFNVPY SYP+FWAAL A
Sbjct: 407 SRSMFWTHISGGLNHQIEHHLFPGVCHVHYPSIQPIVKATCDEFNVPYTSYPTFWAALRA 466

Query: 723 HFQHLKRVGTEN 758
           HFQHLK VG ++
Sbjct: 467 HFQHLKNVGLQD 478


>ref|XP_001766264.1| predicted protein [Physcomitrella patens]
           gi|162682478|gb|EDQ68896.1| predicted protein
           [Physcomitrella patens]
          Length = 480

 Score =  318 bits (814), Expect = 2e-84
 Identities = 153/255 (60%), Positives = 180/255 (70%)
 Frame = +3

Query: 3   GASSFMWRHQHVVGHHSYTNVDNYDPDIRVKDPDVRRVTAKQPIRKYHAFQHVYXXXXXX 182
           GASSFMWR QHVVGHHS+TNVDNYDPDIRVKDPDVRRV   QP + YHA+QH+Y      
Sbjct: 228 GASSFMWRQQHVVGHHSFTNVDNYDPDIRVKDPDVRRVATTQPRQWYHAYQHIYLAVLYG 287

Query: 183 XXXXXXXXXDDFVAFFQGSIGPVKIPKMTVSEAAVFFGGKIFYAIYMILVPLLYSHHSMS 362
                    DDF+A+F GSIGPVK+ KMT  E  +FF GK+ YA YM ++P +Y  HS  
Sbjct: 288 TLALKSIFLDDFLAYFTGSIGPVKVAKMTPLEFNIFFQGKLLYAFYMFVLPSVYGVHSGG 347

Query: 363 SCIILYVVSQLIAGWVLAFLFQVAHVVSEVEFLAVDTSEGVPKLNKGWAEAQVRSTANFS 542
           + + LYV SQLI GW+LAFLFQVAHVV +V F    T EG  K+  GWA  QV +T +FS
Sbjct: 348 TFLALYVASQLITGWMLAFLFQVAHVVDDVAF---PTPEG-GKVKGGWAAMQVATTTDFS 403

Query: 543 TNSTIWTHISGGLNFQIEHHLFPSFCHLHYPMIHSIVRETCREFNVPYFSYPSFWAALLA 722
             S  W H+SGGLN QIEHHLFP  CH+HYP I  IV +TC+EF+VPY +YP+FW AL A
Sbjct: 404 PRSWFWGHVSGGLNNQIEHHLFPGVCHVHYPAIQPIVEKTCKEFDVPYVAYPTFWTALRA 463

Query: 723 HFQHLKRVGTENFVL 767
           HF HLK+VG   F L
Sbjct: 464 HFAHLKKVGLTEFRL 478


>gb|ADB81956.1| delta 5 desaturase [Lobosphaera incisa] gi|358251492|gb|AEU04699.1|
           fatty acid delta-5-desaturase [Lobosphaera incisa]
          Length = 481

 Score =  285 bits (729), Expect = 1e-74
 Identities = 134/249 (53%), Positives = 170/249 (68%)
 Frame = +3

Query: 3   GASSFMWRHQHVVGHHSYTNVDNYDPDIRVKDPDVRRVTAKQPIRKYHAFQHVYXXXXXX 182
           GASSFMWR QHVVGHH+YTNVD  DPD+RVKDPDVRRVT  QP + Y A+QH+Y      
Sbjct: 224 GASSFMWRQQHVVGHHAYTNVDGQDPDLRVKDPDVRRVTKFQPQQSYQAYQHIYLAFLYG 283

Query: 183 XXXXXXXXXDDFVAFFQGSIGPVKIPKMTVSEAAVFFGGKIFYAIYMILVPLLYSHHSMS 362
                    DDF+A   G+IG VK+ K+T  E  VF+GGK  +  Y +L+P++ S HS  
Sbjct: 284 LLAIKSVLLDDFMALSSGAIGSVKVAKLTPGEKLVFWGGKALWLGYFVLLPVVKSRHSWP 343

Query: 363 SCIILYVVSQLIAGWVLAFLFQVAHVVSEVEFLAVDTSEGVPKLNKGWAEAQVRSTANFS 542
                +++S+ + GW+LAF+FQVAHV S+V +L  D +  VP   +GWA AQ  +TA+F+
Sbjct: 344 LLAACWLLSEFVTGWMLAFMFQVAHVTSDVSYLEADKTGKVP---RGWAAAQAATTADFA 400

Query: 543 TNSTIWTHISGGLNFQIEHHLFPSFCHLHYPMIHSIVRETCREFNVPYFSYPSFWAALLA 722
             S  WT ISGGLN+Q+ HHLFP  CHLHYP I  IV +TC+EFNVPY  YP+F  AL A
Sbjct: 401 HGSWFWTQISGGLNYQVVHHLFPGICHLHYPAIAPIVLDTCKEFNVPYHVYPTFVRALAA 460

Query: 723 HFQHLKRVG 749
           HF+HLK +G
Sbjct: 461 HFKHLKDMG 469


>ref|XP_005650943.1| acyl-CoA dependent delta5-desaturase [Coccomyxa subellipsoidea C-169]
            gi|384252924|gb|EIE26399.1| acyl-CoA dependent
            delta5-desaturase [Coccomyxa subellipsoidea C-169]
          Length = 509

 Score =  268 bits (686), Expect = 1e-69
 Identities = 128/254 (50%), Positives = 166/254 (65%)
 Frame = +3

Query: 3    GASSFMWRHQHVVGHHSYTNVDNYDPDIRVKDPDVRRVTAKQPIRKYHAFQHVYXXXXXX 182
            G SSFMWR QHVVGHH+YTN+   DPDIRV D D RRV    P + +HAFQHVY      
Sbjct: 254  GVSSFMWRQQHVVGHHAYTNLA-CDPDIRVSDCDARRVAPHHPKQPFHAFQHVYLAALYG 312

Query: 183  XXXXXXXXXDDFVAFFQGSIGPVKIPKMTVSEAAVFFGGKIFYAIYMILVPLLYSHHSMS 362
                     DDF A  +GSIG +++ +MT  EA +F+  K  YA Y I +P LYS H   
Sbjct: 313  LLKVKSVLVDDFAALARGSIGQLRLSRMTPGEAFMFWACKFLYAAYYIALPALYSPHGWR 372

Query: 363  SCIILYVVSQLIAGWVLAFLFQVAHVVSEVEFLAVDTSEGVPKLNKGWAEAQVRSTANFS 542
            +   L+++++++AGW+LAFLFQVAH+  EVEFL  D      K+ +GWA  QV +TA+FS
Sbjct: 373  ALAALWIIAEVLAGWMLAFLFQVAHITGEVEFLDSDGG----KVERGWAAGQVATTADFS 428

Query: 543  TNSTIWTHISGGLNFQIEHHLFPSFCHLHYPMIHSIVRETCREFNVPYFSYPSFWAALLA 722
              S +WT  SGGLN+Q+ HHLFP+ CH HYP I  IV +TCRE+N+PY  +P+F +AL A
Sbjct: 429  HGSWLWTQFSGGLNYQVVHHLFPNICHTHYPAIAPIVMDTCREYNIPYKVFPNFRSALWA 488

Query: 723  HFQHLKRVGTENFV 764
            HF HLK +G    +
Sbjct: 489  HFAHLKEMGAAQMI 502


>ref|XP_001420855.1| predicted protein [Ostreococcus lucimarinus CCE9901]
           gi|144581090|gb|ABO99148.1| predicted protein
           [Ostreococcus lucimarinus CCE9901]
           gi|336462646|gb|AEI59764.1| delta-5 desaturase
           [synthetic construct]
          Length = 491

 Score =  268 bits (685), Expect = 2e-69
 Identities = 124/254 (48%), Positives = 162/254 (63%)
 Frame = +3

Query: 3   GASSFMWRHQHVVGHHSYTNVDNYDPDIRVKDPDVRRVTAKQPIRKYHAFQHVYXXXXXX 182
           GASSFMW+ QHV GHH+YTNV+  DPDIR  + DVRRV   QP   YH  QHVY      
Sbjct: 234 GASSFMWKQQHVAGHHAYTNVEGIDPDIRCSEKDVRRVNEHQPHEPYHRVQHVYLALMYG 293

Query: 183 XXXXXXXXXDDFVAFFQGSIGPVKIPKMTVSEAAVFFGGKIFYAIYMILVPLLYSHHSMS 362
                    DDF AFF G IG VK+ K T  EA  F+G K+ +A Y + +P  YSH S+ 
Sbjct: 294 LLSFKSCFVDDFNAFFSGRIGWVKVMKFTRGEAVAFWGSKLAWAFYYLYLPAKYSHRSIG 353

Query: 363 SCIILYVVSQLIAGWVLAFLFQVAHVVSEVEFLAVDTSEGVPKLNKGWAEAQVRSTANFS 542
             + L+ V++ + GW+LAF+FQVAHVV +V F  ++      +LNKGW EAQ+ ++A+F+
Sbjct: 354 QLLALWTVTEFVTGWLLAFMFQVAHVVGDVHFFRLNEKN---QLNKGWGEAQLMTSADFA 410

Query: 543 TNSTIWTHISGGLNFQIEHHLFPSFCHLHYPMIHSIVRETCREFNVPYFSYPSFWAALLA 722
             S  WTH SGGLN+Q+ HHLFP  CH+HYP +  I++    +  + Y  YP+FW+AL A
Sbjct: 411 HGSKFWTHFSGGLNYQVVHHLFPGVCHVHYPALAPIIKAAADKHGLHYQIYPTFWSALRA 470

Query: 723 HFQHLKRVGTENFV 764
           HF HL RVG E +V
Sbjct: 471 HFSHLARVGHEAYV 484


>ref|XP_003055412.1| predicted protein [Micromonas pusilla CCMP1545]
           gi|226463386|gb|EEH60664.1| predicted protein
           [Micromonas pusilla CCMP1545]
          Length = 464

 Score =  268 bits (684), Expect = 2e-69
 Identities = 129/255 (50%), Positives = 163/255 (63%), Gaps = 1/255 (0%)
 Frame = +3

Query: 3   GASSFMWRHQHVVGHHSYTNVDNYDPDIRVKDP-DVRRVTAKQPIRKYHAFQHVYXXXXX 179
           GASSFMWR QHVVGHH+YTNV+  DPDIR  D  DVRRV   QP  KYH FQHVY     
Sbjct: 206 GASSFMWRQQHVVGHHAYTNVEGVDPDIRCADEGDVRRVNEHQPHEKYHVFQHVYLFFAY 265

Query: 180 XXXXXXXXXXDDFVAFFQGSIGPVKIPKMTVSEAAVFFGGKIFYAIYMILVPLLYSHHSM 359
                     DDF A+  G IG VK+ K T  E+  F+G K+ +A Y +  P   SHHS 
Sbjct: 266 GLLSFKSCFADDFTAWASGKIGWVKVAKFTSGESLSFWGSKLLWAFYYLYYPSTRSHHSA 325

Query: 360 SSCIILYVVSQLIAGWVLAFLFQVAHVVSEVEFLAVDTSEGVPKLNKGWAEAQVRSTANF 539
                L  ++++I GW+LAF+FQVAHVV +V F  + + EG  KLN GW E+Q+ S+A+F
Sbjct: 326 LRLATLVCITEVITGWLLAFMFQVAHVVGDVRFFQL-SKEG--KLNLGWGESQLYSSADF 382

Query: 540 STNSTIWTHISGGLNFQIEHHLFPSFCHLHYPMIHSIVRETCREFNVPYFSYPSFWAALL 719
           +  S  WTH SGGLN+Q+ HHLFP  CH HYP I  ++ +  +E  + Y  YP+FW+AL 
Sbjct: 383 AHGSKFWTHFSGGLNYQVAHHLFPGVCHCHYPAIAPVIMKVAKEHGLEYCVYPTFWSALA 442

Query: 720 AHFQHLKRVGTENFV 764
           AHF HLKRVG + +V
Sbjct: 443 AHFSHLKRVGQKTYV 457


>ref|XP_003082424.1| polyunsaturated fatty acid delta-5-desaturase (ISS) [Ostreococcus
           tauri] gi|116060892|emb|CAL57370.1| polyunsaturated
           fatty acid delta-5-desaturase (ISS) [Ostreococcus tauri]
           gi|336462648|gb|AEI59765.1| delta-5 desaturase
           [synthetic construct]
          Length = 491

 Score =  265 bits (677), Expect = 1e-68
 Identities = 121/254 (47%), Positives = 160/254 (62%)
 Frame = +3

Query: 3   GASSFMWRHQHVVGHHSYTNVDNYDPDIRVKDPDVRRVTAKQPIRKYHAFQHVYXXXXXX 182
           GASSFMW+ QHV GHH+YTNV+  DPDIR  + D+RRV   QP   YH FQHVY      
Sbjct: 234 GASSFMWKQQHVAGHHAYTNVEGIDPDIRCSEKDIRRVNEHQPHEPYHVFQHVYLAFMYG 293

Query: 183 XXXXXXXXXDDFVAFFQGSIGPVKIPKMTVSEAAVFFGGKIFYAIYMILVPLLYSHHSMS 362
                    DDF A+F G IG VK+ K T  EA  F+G K+ +A Y + +PL  SH  + 
Sbjct: 294 LLSLKSCFVDDFNAYFSGRIGWVKVMKFTRGEAIAFWGTKLLWAAYYLALPLKMSHRPLG 353

Query: 363 SCIILYVVSQLIAGWVLAFLFQVAHVVSEVEFLAVDTSEGVPKLNKGWAEAQVRSTANFS 542
             + L+ V++ + GW+LAF+FQVAHVV EV F  +D      ++N GW EAQ+ S+A+F+
Sbjct: 354 ELLALWAVTEFVTGWLLAFMFQVAHVVGEVHFFTLDAKN---RVNLGWGEAQLMSSADFA 410

Query: 543 TNSTIWTHISGGLNFQIEHHLFPSFCHLHYPMIHSIVRETCREFNVPYFSYPSFWAALLA 722
             S  WTH SGGLN+Q+ HHLFP  CH+HYP +  I++    +  + Y  YP+FW+AL A
Sbjct: 411 HGSKFWTHFSGGLNYQVVHHLFPGVCHVHYPALAPIIKAAAEKHGLHYQIYPTFWSALRA 470

Query: 723 HFQHLKRVGTENFV 764
           HF+HL  VG   +V
Sbjct: 471 HFRHLANVGRAAYV 484


>emb|CAQ30478.1| Acyl-CoA dependent delta5-desaturase [Mantoniella squamata]
          Length = 482

 Score =  260 bits (665), Expect = 3e-67
 Identities = 125/255 (49%), Positives = 162/255 (63%), Gaps = 1/255 (0%)
 Frame = +3

Query: 3   GASSFMWRHQHVVGHHSYTNVDNYDPDIR-VKDPDVRRVTAKQPIRKYHAFQHVYXXXXX 179
           GASSFMWR QHVVGHH+YTNV+  DPDIR   + D+RRV   QP   YH  QHVY     
Sbjct: 224 GASSFMWRQQHVVGHHAYTNVEGVDPDIRCAPEKDIRRVNEHQPHESYHPLQHVYLFFAY 283

Query: 180 XXXXXXXXXXDDFVAFFQGSIGPVKIPKMTVSEAAVFFGGKIFYAIYMILVPLLYSHHSM 359
                     DDF A+  G IG VK+ K T  EA  F+G K+ +A Y + +P  YS HS 
Sbjct: 284 GLLSFKSCFADDFNAWASGRIGWVKVAKFTRGEAVSFWGSKVLWAFYYLYLPATYSPHSG 343

Query: 360 SSCIILYVVSQLIAGWVLAFLFQVAHVVSEVEFLAVDTSEGVPKLNKGWAEAQVRSTANF 539
              + L  ++++I GW+LAF+FQVAHVV +V F  + + EG  KLN GW E+Q+ S+A+F
Sbjct: 344 LRIVALVTITEVITGWLLAFMFQVAHVVGDVRFFKL-SEEG--KLNLGWGESQLYSSADF 400

Query: 540 STNSTIWTHISGGLNFQIEHHLFPSFCHLHYPMIHSIVRETCREFNVPYFSYPSFWAALL 719
           +  S  W H SGGLN+Q+ HHLFP  CH HYP I  I+ +  +E+ + Y  YP+FW+AL 
Sbjct: 401 AHGSKFWMHFSGGLNYQVAHHLFPGVCHCHYPAIAPIIMKVAKEYGLEYAVYPTFWSALS 460

Query: 720 AHFQHLKRVGTENFV 764
           AHF HLK VG + +V
Sbjct: 461 AHFTHLKNVGQKTYV 475


>ref|XP_002507174.1| predicted protein [Micromonas sp. RCC299]
           gi|226522449|gb|ACO68432.1| predicted protein
           [Micromonas sp. RCC299]
          Length = 480

 Score =  255 bits (652), Expect = 1e-65
 Identities = 121/255 (47%), Positives = 165/255 (64%), Gaps = 1/255 (0%)
 Frame = +3

Query: 3   GASSFMWRHQHVVGHHSYTNVDNYDPDIR-VKDPDVRRVTAKQPIRKYHAFQHVYXXXXX 179
           GASSFMWR QHVVGHH+YTNV   DPDIR   + D+RR+   QP   YH +QH+Y     
Sbjct: 222 GASSFMWRQQHVVGHHAYTNVIEVDPDIRCAPEKDLRRINEHQPHETYHCYQHIYLVFAY 281

Query: 180 XXXXXXXXXXDDFVAFFQGSIGPVKIPKMTVSEAAVFFGGKIFYAIYMILVPLLYSHHSM 359
                     DDF A   G IG VK+ K T  EA  F+G K+ +A Y + +P +Y  HS 
Sbjct: 282 GLLSLKSCFVDDFSALASGKIGWVKVAKFTNHEAMTFWGCKLIWAFYYLYLPTIYGTHSA 341

Query: 360 SSCIILYVVSQLIAGWVLAFLFQVAHVVSEVEFLAVDTSEGVPKLNKGWAEAQVRSTANF 539
           S   +L +V++ + GW+LAF+FQVAHVV +V F  + T++G  KL  GW E+Q+ S+A+F
Sbjct: 342 SHAALLIIVTEFVTGWLLAFMFQVAHVVGDVRFFKL-TAQGSLKL--GWGESQLYSSADF 398

Query: 540 STNSTIWTHISGGLNFQIEHHLFPSFCHLHYPMIHSIVRETCREFNVPYFSYPSFWAALL 719
           +  S  WTH SGGLN+Q+ HHLFP  CH +YP I  I+++  +E+ + Y  YPSFW AL+
Sbjct: 399 AHGSWFWTHFSGGLNYQVAHHLFPGVCHCYYPHIAPIIQKVAKEYGLDYCVYPSFWTALV 458

Query: 720 AHFQHLKRVGTENFV 764
           AHF++LK +G ++ V
Sbjct: 459 AHFKYLKVIGQKSHV 473


>emb|CCO66066.1| predicted protein [Bathycoccus prasinos]
          Length = 528

 Score =  251 bits (642), Expect = 1e-64
 Identities = 122/252 (48%), Positives = 157/252 (62%), Gaps = 3/252 (1%)
 Frame = +3

Query: 3    GASSFMWRHQHVVGHHSYTNVDNYDPDIRVKDP-DVRRVTAKQPIRKYHAFQHVYXXXXX 179
            GASSFMW+ QHVVGHH++TNV++ DPDIR  +  DVRRV   QP   +H  QHVY     
Sbjct: 264  GASSFMWKQQHVVGHHAFTNVEDIDPDIRCDEKNDVRRVNYMQPHAFFHKAQHVYLALMY 323

Query: 180  XXXXXXXXXXDDFVAFFQGSIGPVKIPKMTVSEAAVFFGGKIFYAIYMILVPLLYSHH-- 353
                      DDF A     IG V +PK    EA  F+G K  +A Y + +P  YS H  
Sbjct: 324  GLLSFKSCFFDDFSARKNNKIGWVTVPKFERREAVEFWGSKAVWAFYYLYLPFKYSPHND 383

Query: 354  SMSSCIILYVVSQLIAGWVLAFLFQVAHVVSEVEFLAVDTSEGVPKLNKGWAEAQVRSTA 533
            S +    L+ +++   GW+LAF+FQVAHV  +VEF  VD   GV   NK WAEAQ+ S+A
Sbjct: 384  SYAKLFALWTLTEFTTGWLLAFMFQVAHVTPDVEFFKVDEKTGVISSNKSWAEAQLASSA 443

Query: 534  NFSTNSTIWTHISGGLNFQIEHHLFPSFCHLHYPMIHSIVRETCREFNVPYFSYPSFWAA 713
            +F+  S  WTH SGGLN+Q+ HHLFP  CH+HYP +  +V + C++  + Y  YP+FWAA
Sbjct: 444  DFAHGSKFWTHFSGGLNYQVIHHLFPGVCHVHYPQLAPLVMDVCKKRGLEYVVYPTFWAA 503

Query: 714  LLAHFQHLKRVG 749
            L AHF+HLK VG
Sbjct: 504  LRAHFRHLKNVG 515


>gb|ADM78345.1| sphingolipid desaturase-like protein, partial [Picea sitchensis]
           gi|306018587|gb|ADM78347.1| sphingolipid desaturase-like
           protein, partial [Picea sitchensis]
           gi|306018589|gb|ADM78348.1| sphingolipid desaturase-like
           protein, partial [Picea sitchensis]
           gi|306018591|gb|ADM78349.1| sphingolipid desaturase-like
           protein, partial [Picea sitchensis]
           gi|306018593|gb|ADM78350.1| sphingolipid desaturase-like
           protein, partial [Picea sitchensis]
           gi|306018595|gb|ADM78351.1| sphingolipid desaturase-like
           protein, partial [Picea sitchensis]
           gi|306018597|gb|ADM78352.1| sphingolipid desaturase-like
           protein, partial [Picea sitchensis]
           gi|306018599|gb|ADM78353.1| sphingolipid desaturase-like
           protein, partial [Picea sitchensis]
           gi|306018601|gb|ADM78354.1| sphingolipid desaturase-like
           protein, partial [Picea sitchensis]
           gi|306018603|gb|ADM78355.1| sphingolipid desaturase-like
           protein, partial [Picea sitchensis]
           gi|306018605|gb|ADM78356.1| sphingolipid desaturase-like
           protein, partial [Picea sitchensis]
           gi|306018607|gb|ADM78357.1| sphingolipid desaturase-like
           protein, partial [Picea sitchensis]
           gi|306018609|gb|ADM78358.1| sphingolipid desaturase-like
           protein, partial [Picea sitchensis]
           gi|306018611|gb|ADM78359.1| sphingolipid desaturase-like
           protein, partial [Picea sitchensis]
           gi|306018613|gb|ADM78360.1| sphingolipid desaturase-like
           protein, partial [Picea sitchensis]
           gi|306018615|gb|ADM78361.1| sphingolipid desaturase-like
           protein, partial [Picea sitchensis]
           gi|306018617|gb|ADM78362.1| sphingolipid desaturase-like
           protein, partial [Picea sitchensis]
           gi|306018619|gb|ADM78363.1| sphingolipid desaturase-like
           protein, partial [Picea sitchensis]
           gi|306018621|gb|ADM78364.1| sphingolipid desaturase-like
           protein, partial [Picea sitchensis]
           gi|306018623|gb|ADM78365.1| sphingolipid desaturase-like
           protein, partial [Picea sitchensis]
           gi|306018625|gb|ADM78366.1| sphingolipid desaturase-like
           protein, partial [Picea sitchensis]
           gi|306018627|gb|ADM78367.1| sphingolipid desaturase-like
           protein, partial [Picea sitchensis]
           gi|306018629|gb|ADM78368.1| sphingolipid desaturase-like
           protein, partial [Picea sitchensis]
           gi|306018631|gb|ADM78369.1| sphingolipid desaturase-like
           protein, partial [Picea sitchensis]
           gi|306018633|gb|ADM78370.1| sphingolipid desaturase-like
           protein, partial [Picea sitchensis]
           gi|306018635|gb|ADM78371.1| sphingolipid desaturase-like
           protein, partial [Picea sitchensis]
           gi|306018637|gb|ADM78372.1| sphingolipid desaturase-like
           protein, partial [Picea sitchensis]
           gi|306018639|gb|ADM78373.1| sphingolipid desaturase-like
           protein, partial [Picea sitchensis]
           gi|306018643|gb|ADM78375.1| sphingolipid desaturase-like
           protein, partial [Picea sitchensis]
           gi|306018645|gb|ADM78376.1| sphingolipid desaturase-like
           protein, partial [Picea sitchensis]
           gi|306018647|gb|ADM78377.1| sphingolipid desaturase-like
           protein, partial [Picea sitchensis]
           gi|306018649|gb|ADM78378.1| sphingolipid desaturase-like
           protein, partial [Picea sitchensis]
           gi|306018651|gb|ADM78379.1| sphingolipid desaturase-like
           protein, partial [Picea sitchensis]
           gi|306018653|gb|ADM78380.1| sphingolipid desaturase-like
           protein, partial [Picea sitchensis]
           gi|306018655|gb|ADM78381.1| sphingolipid desaturase-like
           protein, partial [Picea sitchensis]
           gi|306018657|gb|ADM78382.1| sphingolipid desaturase-like
           protein, partial [Picea sitchensis]
           gi|306018659|gb|ADM78383.1| sphingolipid desaturase-like
           protein, partial [Picea sitchensis]
           gi|306018661|gb|ADM78384.1| sphingolipid desaturase-like
           protein, partial [Picea sitchensis]
           gi|306018663|gb|ADM78385.1| sphingolipid desaturase-like
           protein, partial [Picea sitchensis]
           gi|306018665|gb|ADM78386.1| sphingolipid desaturase-like
           protein, partial [Picea sitchensis]
           gi|306018667|gb|ADM78387.1| sphingolipid desaturase-like
           protein, partial [Picea sitchensis]
           gi|306018673|gb|ADM78390.1| sphingolipid desaturase-like
           protein, partial [Picea sitchensis]
           gi|306018675|gb|ADM78391.1| sphingolipid desaturase-like
           protein, partial [Picea sitchensis]
          Length = 164

 Score =  246 bits (628), Expect = 6e-63
 Identities = 108/163 (66%), Positives = 135/163 (82%)
 Frame = +3

Query: 288 FFGGKIFYAIYMILVPLLYSHHSMSSCIILYVVSQLIAGWVLAFLFQVAHVVSEVEFLAV 467
           F GGKI Y +YM ++P  +SHH+++  +ILY+VSQL+ GW LAFLFQ+AHVV E  F  V
Sbjct: 1   FIGGKILYTLYMFVLPSFFSHHNVALRVILYIVSQLVCGWTLAFLFQLAHVVPEAAFPVV 60

Query: 468 DTSEGVPKLNKGWAEAQVRSTANFSTNSTIWTHISGGLNFQIEHHLFPSFCHLHYPMIHS 647
           D+S+G PKL++GWA  QVR+T NFSTNS +WTH+SGGLN+QIEHHLFPS CHL+YP IHS
Sbjct: 61  DSSDGRPKLHQGWAAMQVRTTTNFSTNSMLWTHLSGGLNYQIEHHLFPSVCHLYYPSIHS 120

Query: 648 IVRETCREFNVPYFSYPSFWAALLAHFQHLKRVGTENFVLRVS 776
           IV+ETC++F+VPY SYPSFW AL AHF HLK+VG+ENF L +S
Sbjct: 121 IVKETCKDFDVPYHSYPSFWTALKAHFLHLKKVGSENFELHLS 163


>gb|ADM78344.1| sphingolipid desaturase-like protein, partial [Picea sitchensis]
          Length = 164

 Score =  246 bits (628), Expect = 6e-63
 Identities = 108/163 (66%), Positives = 135/163 (82%)
 Frame = +3

Query: 288 FFGGKIFYAIYMILVPLLYSHHSMSSCIILYVVSQLIAGWVLAFLFQVAHVVSEVEFLAV 467
           F GGKI Y +YM ++P  +SHH+++  +ILY+VSQL+ GW LAFLFQ+AHVV E  F  V
Sbjct: 1   FIGGKILYTLYMFVLPSFFSHHNVALRVILYIVSQLVCGWTLAFLFQLAHVVPEAAFPVV 60

Query: 468 DTSEGVPKLNKGWAEAQVRSTANFSTNSTIWTHISGGLNFQIEHHLFPSFCHLHYPMIHS 647
           D+S+G PKL++GWA  QVR+T NFSTNS +WTH+SGGLN+QIEHHLFPS CHL+YP IHS
Sbjct: 61  DSSDGRPKLHQGWAAMQVRTTTNFSTNSMLWTHLSGGLNYQIEHHLFPSVCHLYYPRIHS 120

Query: 648 IVRETCREFNVPYFSYPSFWAALLAHFQHLKRVGTENFVLRVS 776
           IV+ETC++F+VPY SYPSFW AL AHF HLK+VG+ENF L +S
Sbjct: 121 IVKETCKDFDVPYHSYPSFWTALKAHFLHLKKVGSENFELHLS 163


>gb|ADM78346.1| sphingolipid desaturase-like protein, partial [Picea sitchensis]
          Length = 164

 Score =  246 bits (627), Expect = 8e-63
 Identities = 108/163 (66%), Positives = 135/163 (82%)
 Frame = +3

Query: 288 FFGGKIFYAIYMILVPLLYSHHSMSSCIILYVVSQLIAGWVLAFLFQVAHVVSEVEFLAV 467
           F GGKI Y +YM ++P  +SHH+++  +ILY+VSQL+ GW LAFLFQ+AHVV E  F  V
Sbjct: 1   FIGGKILYILYMFVLPSFFSHHNVALRVILYIVSQLVCGWTLAFLFQLAHVVPEAAFPVV 60

Query: 468 DTSEGVPKLNKGWAEAQVRSTANFSTNSTIWTHISGGLNFQIEHHLFPSFCHLHYPMIHS 647
           D+S+G PKL++GWA  QVR+T NFSTNS +WTH+SGGLN+QIEHHLFPS CHL+YP IHS
Sbjct: 61  DSSDGRPKLHQGWAAMQVRTTTNFSTNSMLWTHLSGGLNYQIEHHLFPSVCHLYYPSIHS 120

Query: 648 IVRETCREFNVPYFSYPSFWAALLAHFQHLKRVGTENFVLRVS 776
           IV+ETC++F+VPY SYPSFW AL AHF HLK+VG+ENF L +S
Sbjct: 121 IVKETCKDFDVPYHSYPSFWTALKAHFLHLKKVGSENFELHLS 163


>gb|ADM78388.1| sphingolipid desaturase-like protein, partial [Picea sitchensis]
           gi|306018671|gb|ADM78389.1| sphingolipid desaturase-like
           protein, partial [Picea sitchensis]
          Length = 164

 Score =  245 bits (625), Expect = 1e-62
 Identities = 107/163 (65%), Positives = 135/163 (82%)
 Frame = +3

Query: 288 FFGGKIFYAIYMILVPLLYSHHSMSSCIILYVVSQLIAGWVLAFLFQVAHVVSEVEFLAV 467
           F GGKI Y +YM ++P  +SHH+++  +ILY+VSQL+ GW LAFLFQ+AHVV E  F  V
Sbjct: 1   FIGGKILYTLYMFVLPSFFSHHNVALRVILYIVSQLVCGWTLAFLFQLAHVVPEAAFPVV 60

Query: 468 DTSEGVPKLNKGWAEAQVRSTANFSTNSTIWTHISGGLNFQIEHHLFPSFCHLHYPMIHS 647
           D+S+G PKL++GWA  QVR+T NFSTNS +WTH+SGGLN+QIEHHLFPS CHL+YP IHS
Sbjct: 61  DSSDGRPKLHQGWAAMQVRTTTNFSTNSMLWTHLSGGLNYQIEHHLFPSVCHLYYPSIHS 120

Query: 648 IVRETCREFNVPYFSYPSFWAALLAHFQHLKRVGTENFVLRVS 776
           IV+ETC++F+VPY SYPSFW AL AHF HLK+VG++NF L +S
Sbjct: 121 IVKETCKDFDVPYHSYPSFWTALKAHFLHLKKVGSDNFELHLS 163


>gb|ADM78374.1| sphingolipid desaturase-like protein, partial [Picea sitchensis]
          Length = 164

 Score =  244 bits (623), Expect = 2e-62
 Identities = 108/163 (66%), Positives = 134/163 (82%)
 Frame = +3

Query: 288 FFGGKIFYAIYMILVPLLYSHHSMSSCIILYVVSQLIAGWVLAFLFQVAHVVSEVEFLAV 467
           F GGKI Y +YM ++P  +SHH+++  +ILY+VSQL+ GW LAFLFQ+AHVV E  F  V
Sbjct: 1   FIGGKILYTLYMFVLPSFFSHHNVALRVILYIVSQLVCGWTLAFLFQLAHVVPEAAFPVV 60

Query: 468 DTSEGVPKLNKGWAEAQVRSTANFSTNSTIWTHISGGLNFQIEHHLFPSFCHLHYPMIHS 647
           D+S+G PKL++GWA  QVR+T NFSTNS +WTH+SGGLN QIEHHLFPS CHL+YP IHS
Sbjct: 61  DSSDGRPKLHQGWAAMQVRTTTNFSTNSMLWTHLSGGLNCQIEHHLFPSVCHLYYPSIHS 120

Query: 648 IVRETCREFNVPYFSYPSFWAALLAHFQHLKRVGTENFVLRVS 776
           IV+ETC++F+VPY SYPSFW AL AHF HLK+VG+ENF L +S
Sbjct: 121 IVKETCKDFDVPYHSYPSFWTALKAHFLHLKKVGSENFELHLS 163


>ref|XP_002682732.1| polyunsaturated fatty acid delta-5-desaturase [Naegleria gruberi]
           gi|284096360|gb|EFC49988.1| polyunsaturated fatty acid
           delta-5-desaturase [Naegleria gruberi]
          Length = 443

 Score =  190 bits (483), Expect = 4e-46
 Identities = 91/251 (36%), Positives = 143/251 (56%)
 Frame = +3

Query: 3   GASSFMWRHQHVVGHHSYTNVDNYDPDIRVKDPDVRRVTAKQPIRKYHAFQHVYXXXXXX 182
           G SSF W+  H VGHH  TNV+  DPDI   +P  R++   Q    ++++QH+Y      
Sbjct: 187 GGSSFTWKMIHSVGHHVNTNVEERDPDIHTNEPHFRKIKEGQKQHWWYSYQHIYLPFLYC 246

Query: 183 XXXXXXXXXDDFVAFFQGSIGPVKIPKMTVSEAAVFFGGKIFYAIYMILVPLLYSHHSMS 362
                    D F A   G+ G VK      +E  +F   K+ +  Y +  PL++SHHS +
Sbjct: 247 TLLFELAFRD-FAAMMIGAWGGVKFQPAPKNEYLLFVSSKMCWLTYSLAFPLMFSHHSTA 305

Query: 363 SCIILYVVSQLIAGWVLAFLFQVAHVVSEVEFLAVDTSEGVPKLNKGWAEAQVRSTANFS 542
           +  +L+ ++ ++A ++L  +FQV HV    +   V+ ++G+  ++  W   QV  ++NF+
Sbjct: 306 TVFMLWTIAYMVASFILVLMFQVNHVTPLTDTYHVE-NDGM--VHADWGVTQVSGSSNFA 362

Query: 543 TNSTIWTHISGGLNFQIEHHLFPSFCHLHYPMIHSIVRETCREFNVPYFSYPSFWAALLA 722
             S +W HISGGL  Q EHHLFP+ CH+HYP I  IV +  +++N+ Y +YPSFW A++ 
Sbjct: 363 CRSWLWNHISGGLCHQTEHHLFPTICHIHYPQIQPIVEKCAKKYNIRYNAYPSFWDAVVG 422

Query: 723 HFQHLKRVGTE 755
           HF  LK +G +
Sbjct: 423 HFTLLKEMGAQ 433


>ref|XP_003292036.1| hypothetical protein DICPUDRAFT_39933 [Dictyostelium purpureum]
           gi|325077744|gb|EGC31437.1| hypothetical protein
           DICPUDRAFT_39933 [Dictyostelium purpureum]
          Length = 454

 Score =  188 bits (477), Expect = 2e-45
 Identities = 96/252 (38%), Positives = 142/252 (56%), Gaps = 1/252 (0%)
 Frame = +3

Query: 3   GASSFMWRHQHVVGHHSYTNVDNYDPDIRVKDPDVRRVTAKQPIRKYHAFQHVYXXXXXX 182
           GAS + W HQHV+GHH YTN+ N DPD+   + D R VT  QP   YH +QH+Y      
Sbjct: 193 GASFYAWCHQHVIGHHLYTNIRNADPDLGEGEIDFRIVTPYQPRSWYHKYQHIYAPILYG 252

Query: 183 XXXXXXXXXDDFVAFFQGSIGPVKIPKMTVSEAAVFFGGKIFYAIYMILVPLLYSHHSMS 362
                     D   F +G+ G +++   T  + + F  GK+ Y  +   +PL Y  HS S
Sbjct: 253 LYSFKYHL-QDHETFTKGTNGSIRVSPPTTFDYSAFILGKLSYVFFRFYLPLQY--HSFS 309

Query: 363 SCIILYVVSQLIAGWVLAFLFQVAHVVSEVEFLAVDTS-EGVPKLNKGWAEAQVRSTANF 539
           + II  ++++ I GW LA  FQV+HV  +V+F A     E   ++N+ WA  QV++T ++
Sbjct: 310 NLIIYIIITESILGWYLALGFQVSHVAEDVKFYATPQKPEEAEQINEDWAILQVKTTQDY 369

Query: 540 STNSTIWTHISGGLNFQIEHHLFPSFCHLHYPMIHSIVRETCREFNVPYFSYPSFWAALL 719
              S   T +SG LN+Q+ HHLFPS     YP I  IV+E C+E+NV Y    +F+ AL+
Sbjct: 370 GHGSIACTFLSGSLNYQVVHHLFPSIDQEFYPQIAPIVKEVCKEYNVKYHIKETFYEALM 429

Query: 720 AHFQHLKRVGTE 755
           +H  +L ++G +
Sbjct: 430 SHINYLYKMGND 441