BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Ephedra28_contig00019178
         (734 letters)

Database: ./nr 
           37,332,560 sequences; 13,225,080,153 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AHF65153.1| stylopine synthase, partial [synthetic construct]      154   2e-35
gb|ADB89214.1| stylopine synthase [Papaver somniferum]                154   2e-35
sp|B5UAQ8.1|C7195_ESCCA RecName: Full=Cheilanthifoline synthase;...   152   1e-34
gb|AFI57927.1| cytochrome P450, partial [Papaver somniferum]          148   2e-33
sp|B1NF19.1|C719D_ARGME RecName: Full=(S)-stylopine synthase; Sh...   148   2e-33
gb|AFB74615.1| cytochrome P450 [Papaver somniferum]                   147   3e-33
gb|ADB89213.1| cheilanthifoline synthase [Papaver somniferum]         146   6e-33
sp|B1NF20.1|C719E_ARGME RecName: Full=Cheilanthifoline synthase;...   145   1e-32
sp|B1NF18.1|C719B_PAPSO RecName: Full=Salutaridine synthase; Alt...   145   1e-32
sp|Q50LH3.1|C7192_ESCCA RecName: Full=(S)-stylopine synthase 1; ...   144   3e-32
dbj|BAG75114.1| cytochrome P450 [Eschscholzia californica]            143   5e-32
dbj|BAF98470.1| cytochrome P450 [Coptis japonica var. dissecta]       143   7e-32
gb|AAU20771.1| (S)-canadine synthase [Thalictrum flavum subsp. g...   142   1e-31
gb|ACO90219.1| stylopine synthase [Eschscholzia californica]          141   2e-31
gb|AGL76711.1| (S)-canadine synthase, partial [Coptis chinensis]      141   3e-31
sp|Q948Y1.1|C719A_COPJA RecName: Full=(S)-canadine synthase; Alt...   141   3e-31
sp|Q50EK4.1|C75A1_PINTA RecName: Full=Cytochrome P450 750A1; Alt...   140   6e-31
ref|XP_002978564.1| hypothetical protein SELMODRAFT_108700 [Sela...   139   7e-31
ref|XP_002970487.1| hypothetical protein SELMODRAFT_93385 [Selag...   139   1e-30
dbj|BAG75116.1| cytochrome P450 [Eschscholzia californica]            139   1e-30

>gb|AHF65153.1| stylopine synthase, partial [synthetic construct]
          Length = 511

 Score =  154 bits (390), Expect = 2e-35
 Identities = 93/223 (41%), Positives = 134/223 (60%), Gaps = 8/223 (3%)
 Frame = -2

Query: 727 EEIAFNVFEIFFLGVDSTATSMEWALAYFVAHPEMQNKAFNEIQTVTPSKKD---VAMED 557
           E I F +FE + LGVDST+ +  WALA+ +  P +Q+K + E++ +T +K D   V +ED
Sbjct: 297 EVIIFAIFEAYLLGVDSTSLTTAWALAFLIREPVVQDKLYQELKNLT-AKNDCEIVKVED 355

Query: 556 VSKLTYLEAVLKEVLRMHAIAPFAIPHRSTRKTILGGFVVPANTTVLFNIDSVAHDPKLW 377
           ++KL YL+AVLKE +RM  IAP AIPH++ R T L G  +   T V+ NI ++ H+ K+W
Sbjct: 356 LNKLQYLQAVLKETMRMKPIAPLAIPHKACRDTSLKGNKIDQGTQVMVNIYALHHNEKVW 415

Query: 376 GEDEHELDKFKAQRFMK---MAESKGKERGANPMCENISFGAGRRQCAGMELANMLVPLT 206
            E      KF  +RF++   +   KG E+   P      F AG R CAGMEL  +    +
Sbjct: 416 KEP----FKFMPERFLQTQDVVNGKGMEQSLLP------FSAGMRICAGMELGKLQFSFS 465

Query: 205 LANLLKAFEWTCETSP--PDLTPYCPFMLRSLKKPLRARIFHR 83
           LANL+ AFEW+C +    PD++    F+L  +K PL+ARI  R
Sbjct: 466 LANLVNAFEWSCVSDGVLPDMSDQLGFVL-LMKTPLQARIVPR 507


>gb|ADB89214.1| stylopine synthase [Papaver somniferum]
          Length = 494

 Score =  154 bits (390), Expect = 2e-35
 Identities = 93/223 (41%), Positives = 134/223 (60%), Gaps = 8/223 (3%)
 Frame = -2

Query: 727 EEIAFNVFEIFFLGVDSTATSMEWALAYFVAHPEMQNKAFNEIQTVTPSKKD---VAMED 557
           E I F +FE + LGVDST+ +  WALA+ +  P +Q+K + E++ +T +K D   V +ED
Sbjct: 283 EVIIFAIFEAYLLGVDSTSLTTAWALAFLIREPVVQDKLYQELKNLT-AKNDCEIVKVED 341

Query: 556 VSKLTYLEAVLKEVLRMHAIAPFAIPHRSTRKTILGGFVVPANTTVLFNIDSVAHDPKLW 377
           ++KL YL+AVLKE +RM  IAP AIPH++ R T L G  +   T V+ NI ++ H+ K+W
Sbjct: 342 LNKLQYLQAVLKETMRMKPIAPLAIPHKACRDTSLKGNKIDQGTQVMVNIYALHHNEKVW 401

Query: 376 GEDEHELDKFKAQRFMK---MAESKGKERGANPMCENISFGAGRRQCAGMELANMLVPLT 206
            E      KF  +RF++   +   KG E+   P      F AG R CAGMEL  +    +
Sbjct: 402 KEP----FKFMPERFLQTQDVVNGKGMEQSLLP------FSAGMRICAGMELGKLQFSFS 451

Query: 205 LANLLKAFEWTCETSP--PDLTPYCPFMLRSLKKPLRARIFHR 83
           LANL+ AFEW+C +    PD++    F+L  +K PL+ARI  R
Sbjct: 452 LANLVNAFEWSCVSDGVLPDMSDQLGFVL-LMKTPLQARIVPR 493


>sp|B5UAQ8.1|C7195_ESCCA RecName: Full=Cheilanthifoline synthase; Short=CHS; AltName:
           Full=Cytochrome P450 719A5 gi|209571368|dbj|BAG75113.1|
           cheilanthifoline synthase [Eschscholzia californica]
          Length = 490

 Score =  152 bits (384), Expect = 1e-34
 Identities = 92/220 (41%), Positives = 137/220 (62%), Gaps = 3/220 (1%)
 Frame = -2

Query: 733 TNEEIAFNVFEIFFLGVDSTATSMEWALAYFVAHPEMQNKAFNEIQTVTPSKKDVAMEDV 554
           ++E I   V E++ LGVDSTA++  WAL + V  P +Q K + EI  +T  ++ V +EDV
Sbjct: 275 SDEVIISAVLEVYDLGVDSTASTAVWALTFLVREPRVQEKLYKEIIDLTGGERSVKVEDV 334

Query: 553 SKLTYLEAVLKEVLRMHAIAPFAIPHRSTRKTILGGFVVPANTTVLFNIDSVAHDPKLWG 374
           SKL YL+AV+KE +RM  IAP AIPH+++R T L G  V   T+++ N+ ++ H+PK++ 
Sbjct: 335 SKLPYLQAVMKETMRMKPIAPMAIPHKTSRDTSLMGKKVNKGTSIMVNLYAIHHNPKVFP 394

Query: 373 EDEHELDKFKAQRFMKMAESKGKERGANPMCEN-ISFGAGRRQCAGMELANMLVPLTLAN 197
           E      KF  +RF++  ESK  +     M ++ + F AG R CAGMEL  +    +LA+
Sbjct: 395 EPY----KFIPERFLQGQESKYGD--IKEMEQSLLPFSAGMRICAGMELGKLQYGFSLAS 448

Query: 196 LLKAFEWTC--ETSPPDLTPYCPFMLRSLKKPLRARIFHR 83
           L++AF+WTC  +   PDL+    F+L  +K PL ARI  R
Sbjct: 449 LVEAFKWTCAVDGKLPDLSEDHCFIL-LMKNPLEARITPR 487


>gb|AFI57927.1| cytochrome P450, partial [Papaver somniferum]
          Length = 488

 Score =  148 bits (374), Expect = 2e-33
 Identities = 88/219 (40%), Positives = 126/219 (57%), Gaps = 4/219 (1%)
 Frame = -2

Query: 727 EEIAFNVFEIFFLGVDSTATSMEWALAYFVAHPEMQNKAFNEIQTVTPSKKDVAM--EDV 554
           E I F +FE + LGVDST+++  WALA+ +  P +Q K + E++  T +     +  EDV
Sbjct: 281 EVIIFAIFEAYLLGVDSTSSTTAWALAFLIREPSVQEKLYQELKNFTANNNRTMLKVEDV 340

Query: 553 SKLTYLEAVLKEVLRMHAIAPFAIPHRSTRKTILGGFVVPANTTVLFNIDSVAHDPKLWG 374
           +KL YL+AV+KE +RM  IAP AIPH++ + T L G  V   T V+ NI ++ H  K+W 
Sbjct: 341 NKLPYLQAVVKETMRMKPIAPLAIPHKACKDTSLMGKKVDKGTKVMVNIHALHHTEKVWK 400

Query: 373 EDEHELDKFKAQRFMKMAESKGKERGANPMCENISFGAGRRQCAGMELANMLVPLTLANL 194
           E      KF  +RF++    K  E+   P      F AG R CAGMEL  +    +LANL
Sbjct: 401 EPY----KFMPERFLQ-KHDKAMEQSLLP------FSAGMRICAGMELGKLQFSFSLANL 449

Query: 193 LKAFEWTCETSP--PDLTPYCPFMLRSLKKPLRARIFHR 83
           + AF+W+C +    PD++    F+L  +K PL ARI  R
Sbjct: 450 VNAFKWSCVSDGVLPDMSDLLGFVL-FMKTPLEARIVPR 487


>sp|B1NF19.1|C719D_ARGME RecName: Full=(S)-stylopine synthase; Short=STS; AltName:
           Full=Cytochrome P450 719A13 gi|148841092|gb|ABR14721.1|
           stylopine synthase [Argemone mexicana]
          Length = 504

 Score =  148 bits (373), Expect = 2e-33
 Identities = 89/224 (39%), Positives = 128/224 (57%), Gaps = 8/224 (3%)
 Frame = -2

Query: 730 NEEIAFNVFEIFFLGVDSTATSMEWALAYFVAHPEMQNKAFNEIQTVTPS--KKDVAMED 557
           +E I F +FE + LGVDST+++  WALAY +  P +Q K + E++  T    +K V  ED
Sbjct: 291 DEVIIFAIFEAYLLGVDSTSSTTAWALAYLIREPNVQEKLYEELKNFTNDNDRKMVKFED 350

Query: 556 VSKLTYLEAVLKEVLRMHAIAPFAIPHRSTRKTILGGFVVPANTTVLFNIDSVAHDPKLW 377
           ++KL YL+AV+KE +RM  IAP AIPH++ R+T L G  V   T V+ NI ++ H+  +W
Sbjct: 351 LNKLQYLQAVVKETMRMKPIAPLAIPHKACRETSLMGRKVNQGTRVMVNIYALHHNQNVW 410

Query: 376 GEDEHELDKFKAQRFMKM----AESKGKERGANPMCENISFGAGRRQCAGMELANMLVPL 209
            E      KF  +RF++      + K  E+   P      F AG R CAGMEL  +    
Sbjct: 411 KEPY----KFNPERFLQKNQDGVDGKAMEQSLLP------FSAGMRICAGMELGKLQFSF 460

Query: 208 TLANLLKAFEWTCETSP--PDLTPYCPFMLRSLKKPLRARIFHR 83
            LANL+ AF+W+C +    PD++    F+L  +K PL A I  R
Sbjct: 461 ALANLVNAFKWSCVSDGVFPDMSDQLGFVL-LMKTPLEAGIVPR 503


>gb|AFB74615.1| cytochrome P450 [Papaver somniferum]
          Length = 486

 Score =  147 bits (372), Expect = 3e-33
 Identities = 88/219 (40%), Positives = 126/219 (57%), Gaps = 4/219 (1%)
 Frame = -2

Query: 727 EEIAFNVFEIFFLGVDSTATSMEWALAYFVAHPEMQNKAFNEIQTVTPSKKDVAM--EDV 554
           E I F +FE + LGVDST+++  WALA+ +  P +Q K + E++  T +     +  EDV
Sbjct: 279 EVIIFAIFEAYLLGVDSTSSTTAWALAFLIREPSVQEKLYQELKNFTANNNRTMLKVEDV 338

Query: 553 SKLTYLEAVLKEVLRMHAIAPFAIPHRSTRKTILGGFVVPANTTVLFNIDSVAHDPKLWG 374
           +KL YL+AV+KE +RM  IAP AIPH++ + T L G  V   T V+ NI ++ H  K+W 
Sbjct: 339 NKLPYLQAVVKETMRMKPIAPLAIPHKACKDTSLMGKKVDKGTKVMVNIHALHHTEKVWK 398

Query: 373 EDEHELDKFKAQRFMKMAESKGKERGANPMCENISFGAGRRQCAGMELANMLVPLTLANL 194
           E      KF  +RF++    K  E+   P      F AG R CAGMEL  +    +LANL
Sbjct: 399 EPY----KFIPERFLQ-KHDKAMEQSLLP------FSAGMRICAGMELGKLQFSFSLANL 447

Query: 193 LKAFEWTCETSP--PDLTPYCPFMLRSLKKPLRARIFHR 83
           + AF+W+C +    PD++    F+L  +K PL ARI  R
Sbjct: 448 VNAFKWSCVSDGVLPDMSDLLGFVL-FMKTPLEARIVPR 485


>gb|ADB89213.1| cheilanthifoline synthase [Papaver somniferum]
          Length = 492

 Score =  146 bits (369), Expect = 6e-33
 Identities = 85/212 (40%), Positives = 126/212 (59%), Gaps = 3/212 (1%)
 Frame = -2

Query: 709 VFEIFFLGVDSTATSMEWALAYFVAHPEMQNKAFNEIQTVTPSKKDVAMEDVSKLTYLEA 530
           + E++ LGVDSTA++  WAL + V   ++Q K + EI  VT  KK V +ED++KL YL+A
Sbjct: 284 ILEVYDLGVDSTASTTVWALTFLVREQKIQEKLYREINNVTGGKKPVKVEDLNKLPYLQA 343

Query: 529 VLKEVLRMHAIAPFAIPHRSTRKTILGGFVVPANTTVLFNIDSVAHDPKLWGEDEHELDK 350
           V+KE +RM  IAP AIPH++++ T L G  +     V+ N+ ++ H+P ++ E      K
Sbjct: 344 VMKETMRMKPIAPMAIPHKASKDTSLMGKKINKGAVVMVNLYAIHHNPAVFPEPY----K 399

Query: 349 FKAQRFMKMAESKGKERGANPMCEN-ISFGAGRRQCAGMELANMLVPLTLANLLKAFEWT 173
           F  +RF+K A S G       M  + ++F AG R CAGMEL  + +   LA+L+  F+W 
Sbjct: 400 FMPERFLKDANSDGSLGDIKKMESSLLAFSAGMRICAGMELGKLQLAFGLASLVNEFKWD 459

Query: 172 C--ETSPPDLTPYCPFMLRSLKKPLRARIFHR 83
           C  E   PDL+    F+L  +K PL A+I  R
Sbjct: 460 CFAEGKLPDLSEEHCFIL-LMKNPLEAKITPR 490


>sp|B1NF20.1|C719E_ARGME RecName: Full=Cheilanthifoline synthase; Short=CHS; AltName:
           Full=Cytochrome P450 719A14 gi|148841094|gb|ABR14722.1|
           cheilanthifoline synthase [Argemone mexicana]
          Length = 494

 Score =  145 bits (367), Expect = 1e-32
 Identities = 84/217 (38%), Positives = 130/217 (59%), Gaps = 4/217 (1%)
 Frame = -2

Query: 730 NEEIAFN-VFEIFFLGVDSTATSMEWALAYFVAHPEMQNKAFNEIQTVTPSKKDVAMEDV 554
           NE+I  + + E++ LGVDSTA++  WAL + V   E+Q K + EI  VT  K+ V +EDV
Sbjct: 278 NEDIIISAILEVYDLGVDSTASTTVWALTFLVREQEIQEKLYREIVNVTGGKRSVKVEDV 337

Query: 553 SKLTYLEAVLKEVLRMHAIAPFAIPHRSTRKTILGGFVVPANTTVLFNIDSVAHDPKLWG 374
           +K+ YL+AV+KE +RM  IAP AIPH++++ T L G  +   + ++ N+ ++ H+PK++ 
Sbjct: 338 NKMPYLQAVMKETMRMKPIAPMAIPHKTSKDTSLMGKKINKGSVIMVNLYAIHHNPKVFP 397

Query: 373 EDEHELDKFKAQRFMKMAESKGKERGANPMCEN-ISFGAGRRQCAGMELANMLVPLTLAN 197
           E      KF  +RF+K   S         M  + ++F AG R CAGMEL  + +   LA+
Sbjct: 398 EPY----KFMPERFLKDVNSDESLGNIKTMESSLLAFSAGMRICAGMELGKLQLAFGLAS 453

Query: 196 LLKAFEWTCETSP--PDLTPYCPFMLRSLKKPLRARI 92
           L+  F+W+C      PDL+    F+L  +K PL A+I
Sbjct: 454 LVHEFKWSCSVDGKLPDLSEDHCFIL-LMKNPLEAKI 489


>sp|B1NF18.1|C719B_PAPSO RecName: Full=Salutaridine synthase; AltName: Full=Cytochrome P450
           719B1 gi|148841090|gb|ABR14720.1| salutaridine synthase
           [Papaver somniferum]
          Length = 505

 Score =  145 bits (367), Expect = 1e-32
 Identities = 87/220 (39%), Positives = 127/220 (57%), Gaps = 4/220 (1%)
 Frame = -2

Query: 730 NEEIAFN-VFEIFFLGVDSTATSMEWALAYFVAHPEMQNKAFNEIQTVTPSKKDVAMEDV 554
           ++EI ++ V EIF LGVDSTA +  WAL++ V   E+Q K + EI   T  ++ V + D+
Sbjct: 285 SDEIIYSMVLEIFGLGVDSTAATAVWALSFLVGEQEIQEKLYREINNRTGGQRPVKVVDL 344

Query: 553 SKLTYLEAVLKEVLRMHAIAPFAIPHRSTRKTILGGFVVPANTTVLFNIDSVAHDPKLWG 374
            +L YL+AV+KE LRM  IAP A+PH + + T   G  +   T V+ N+ ++ HDP ++ 
Sbjct: 345 KELPYLQAVMKETLRMKPIAPLAVPHVAAKDTTFKGRRIVKGTKVMVNLYAIHHDPNVFP 404

Query: 373 EDEHELDKFKAQRFMKMAESKGKERGANPMCEN-ISFGAGRRQCAGMELANMLVPLTLAN 197
                  KF  +RF+K   S G+    N M  + I FGAG R C G+ELA  +V   LA+
Sbjct: 405 APY----KFMPERFLKDVNSDGRFGDINTMESSLIPFGAGMRICGGVELAKQMVAFALAS 460

Query: 196 LLKAFEWTC--ETSPPDLTPYCPFMLRSLKKPLRARIFHR 83
           ++  F+W C  E   PDL+    F+L  +K PL A+I  R
Sbjct: 461 MVNEFKWDCVSEGKLPDLSEAISFIL-YMKNPLEAKITPR 499


>sp|Q50LH3.1|C7192_ESCCA RecName: Full=(S)-stylopine synthase 1; Short=STS; AltName:
           Full=Cytochrome P450 719A2; Short=EcCYP719A2;
           Short=EcCYPB gi|63108256|dbj|BAD98250.1| stylopine
           synthase [Eschscholzia californica]
          Length = 495

 Score =  144 bits (363), Expect = 3e-32
 Identities = 89/225 (39%), Positives = 128/225 (56%), Gaps = 9/225 (4%)
 Frame = -2

Query: 730 NEEIAFNVFEIFFLGVDSTATSMEWALAYFVAHPEMQNKAFNEIQTVTPSKKD---VAME 560
           +E I F +FE + LGVDST+ +  WALA+ +  P +Q K + E+++   SK D   + +E
Sbjct: 282 DEVIIFAIFEAYLLGVDSTSLTTAWALAFLIREPNVQEKLYQELESFA-SKNDRRILKVE 340

Query: 559 DVSKLTYLEAVLKEVLRMHAIAPFAIPHRSTRKTILGGFVVPANTTVLFNIDSVAHDPKL 380
           D++KL YL+AV+KE +RM  IAP AIPH++ R T L G  +   T V+ NI ++ H+  +
Sbjct: 341 DINKLQYLQAVIKETMRMKPIAPLAIPHKACRDTSLMGKKIDKGTRVMVNIFALHHNKNV 400

Query: 379 WGEDEHELDKFKAQRFMKM----AESKGKERGANPMCENISFGAGRRQCAGMELANMLVP 212
           + +      KF  +RFMK+    A  K  E+   P      F AG R CAGMEL  +   
Sbjct: 401 FNDP----FKFMPERFMKVDSQDANGKAMEQSLLP------FSAGMRICAGMELGKLQFS 450

Query: 211 LTLANLLKAFEWTCETSP--PDLTPYCPFMLRSLKKPLRARIFHR 83
             LANL  AF+W+C      PD++    F+L  +K PL ARI  R
Sbjct: 451 FALANLAYAFKWSCVADGVLPDMSDQLGFVL-LMKTPLEARINRR 494


>dbj|BAG75114.1| cytochrome P450 [Eschscholzia californica]
          Length = 495

 Score =  143 bits (361), Expect = 5e-32
 Identities = 85/222 (38%), Positives = 127/222 (57%), Gaps = 7/222 (3%)
 Frame = -2

Query: 727 EEIAFNVFEIFFLGVDSTATSMEWALAYFVAHPEMQNKAFNEIQTVTPS--KKDVAMEDV 554
           E I F++FE++ LGVDST+++  WALAY V  P +Q   + E+        +K + +ED+
Sbjct: 284 ELIIFSIFEVYLLGVDSTSSTTAWALAYLVREPNVQETLYQELDNFAKQNDRKILKVEDI 343

Query: 553 SKLTYLEAVLKEVLRMHAIAPFAIPHRSTRKTILGGFVVPANTTVLFNIDSVAHDPKLWG 374
           +KL YL+AV KE +RM  IAP AIPH+++R T L G  V   T V+ N+ ++ H+  ++ 
Sbjct: 344 NKLQYLQAVTKETMRMKPIAPLAIPHKASRDTTLMGKKVEKGTKVMVNLHALHHNENVFN 403

Query: 373 EDEHELDKFKAQRFMKM---AESKGKERGANPMCENISFGAGRRQCAGMELANMLVPLTL 203
           +      KF  +RF+K    A++K  E+   P      F AG R C GME+  +     L
Sbjct: 404 DPY----KFMPERFLKSYQGAKAKAMEQSYLP------FSAGMRICGGMEVGKLQFGFAL 453

Query: 202 ANLLKAFEWTCETSP--PDLTPYCPFMLRSLKKPLRARIFHR 83
           ANL  AF+W+C  +   PD++    F+L  +K PL ARI  R
Sbjct: 454 ANLAYAFKWSCAVNGVLPDMSDELGFVL-FMKTPLEARIVRR 494


>dbj|BAF98470.1| cytochrome P450 [Coptis japonica var. dissecta]
          Length = 491

 Score =  143 bits (360), Expect = 7e-32
 Identities = 86/223 (38%), Positives = 124/223 (55%), Gaps = 6/223 (2%)
 Frame = -2

Query: 733 TNEEIAFNVFEIFFLGVDSTATSMEWALAYFVAHPEMQNKAFNEIQTVTPSKKDVAMEDV 554
           + E I F  FE+F LGVDST+++  WALAY +    +Q K   +I+        V +ED+
Sbjct: 278 SKEIIIFCTFELFLLGVDSTSSTATWALAYLIHEQAVQEKLNEDIRMTIGDVDLVKIEDL 337

Query: 553 SKLTYLEAVLKEVLRMHAIAPFAIPHRSTRKTILGGFVVPANTTVLFNIDSVAHDPKLWG 374
           SKL YL+AV+KE +RM  IAP AIPH++   T L G  V   T ++ N+ ++ H+  +W 
Sbjct: 338 SKLKYLQAVVKETMRMKPIAPLAIPHKTANDTTLMGTKVARGTQIMVNLYALHHNEDIWP 397

Query: 373 EDEHELDKFKAQRFMK----MAESKGKERGANPMCENISFGAGRRQCAGMELANMLVPLT 206
           E      KF  +RF++     A +K  E+   P      FGAG R CAGM+L  + +   
Sbjct: 398 EPY----KFMPERFLQGENVSANNKAMEQSFLP------FGAGMRICAGMDLGKLQIAFA 447

Query: 205 LANLLKAFEWTC--ETSPPDLTPYCPFMLRSLKKPLRARIFHR 83
           LANL+ AF+W+C  E   PD++    F +  +K PL  RI  R
Sbjct: 448 LANLVNAFKWSCVDEGKLPDMSEQL-FAVTLMKTPLEGRISTR 489


>gb|AAU20771.1| (S)-canadine synthase [Thalictrum flavum subsp. glaucum]
          Length = 492

 Score =  142 bits (358), Expect = 1e-31
 Identities = 84/223 (37%), Positives = 125/223 (56%), Gaps = 6/223 (2%)
 Frame = -2

Query: 733 TNEEIAFNVFEIFFLGVDSTATSMEWALAYFVAHPEMQNKAFNEIQTVTPSKKDVAMEDV 554
           + E I F +FE++ LGVDST+++  WALAY +    +Q K + + +        V +EDV
Sbjct: 279 SEEVIIFCIFELYLLGVDSTSSTTTWALAYLIREQAIQEKLYQDTRMTLGDVDLVKIEDV 338

Query: 553 SKLTYLEAVLKEVLRMHAIAPFAIPHRSTRKTILGGFVVPANTTVLFNIDSVAHDPKLWG 374
           SKL YL+AV+KE +RM  IAP AIPH++ + T L G  V   T ++ N+ ++ H+  +W 
Sbjct: 339 SKLKYLQAVVKETMRMKPIAPLAIPHKTAKDTTLMGSKVAKGTRIMVNLYALHHNQNIWT 398

Query: 373 EDEHELDKFKAQRFMK----MAESKGKERGANPMCENISFGAGRRQCAGMELANMLVPLT 206
                  KF  +RF++     A +K  E+   P      F AG R CAGM+L  +     
Sbjct: 399 NPY----KFMPERFLQGEDGSASNKAMEQSFLP------FSAGMRICAGMDLGKLQFAFA 448

Query: 205 LANLLKAFEWTC--ETSPPDLTPYCPFMLRSLKKPLRARIFHR 83
           LANL+ AF+W+C  E   PD++    F+L  +K PL A+I  R
Sbjct: 449 LANLVNAFKWSCVEEGKFPDMSEELSFVL-LMKTPLEAKITAR 490


>gb|ACO90219.1| stylopine synthase [Eschscholzia californica]
          Length = 495

 Score =  141 bits (356), Expect = 2e-31
 Identities = 86/224 (38%), Positives = 126/224 (56%), Gaps = 8/224 (3%)
 Frame = -2

Query: 730 NEEIAFNVFEIFFLGVDSTATSMEWALAYFVAHPEMQNKAFNEIQTVTPS--KKDVAMED 557
           +E I F +FE + LGVDST+ +  WALA+ +    +Q K + E+++      +K + +ED
Sbjct: 282 DEVIIFAIFEAYLLGVDSTSLTTAWALAFLIRESNVQEKLYQELESFASKMDRKILKVED 341

Query: 556 VSKLTYLEAVLKEVLRMHAIAPFAIPHRSTRKTILGGFVVPANTTVLFNIDSVAHDPKLW 377
           ++KL YL+AV+KE +RM  IAP AIPH++ R T L G  +   T V+ NI ++ H+  ++
Sbjct: 342 INKLQYLQAVIKETMRMKPIAPLAIPHKACRDTSLMGKKIDKGTRVMVNIYALHHNKNVF 401

Query: 376 GEDEHELDKFKAQRFMKM----AESKGKERGANPMCENISFGAGRRQCAGMELANMLVPL 209
            +      KF  +RFMK+    A  K  E+   P      F AG R CAGMEL  +    
Sbjct: 402 NDP----FKFMPERFMKVDSQDANGKAMEQSLLP------FSAGMRICAGMELGKLQFSF 451

Query: 208 TLANLLKAFEWTCETSP--PDLTPYCPFMLRSLKKPLRARIFHR 83
            LANL  AF+W+C      PD++    F+L  +K PL ARI  R
Sbjct: 452 ALANLAYAFKWSCVADGVLPDMSDQLGFVL-LMKTPLEARINRR 494


>gb|AGL76711.1| (S)-canadine synthase, partial [Coptis chinensis]
          Length = 491

 Score =  141 bits (355), Expect = 3e-31
 Identities = 81/217 (37%), Positives = 121/217 (55%), Gaps = 2/217 (0%)
 Frame = -2

Query: 727 EEIAFNVFEIFFLGVDSTATSMEWALAYFVAHPEMQNKAFNEIQTVTPSKKDVAMEDVSK 548
           E I F +FE++ LGVDST+++  WALAY +     Q K + +I+        V +EDV+K
Sbjct: 280 EVIIFCIFELYLLGVDSTSSTTTWALAYLIREQGAQEKLYQDIRMTLGDVDLVKIEDVNK 339

Query: 547 LTYLEAVLKEVLRMHAIAPFAIPHRSTRKTILGGFVVPANTTVLFNIDSVAHDPKLWGED 368
           L YL+ V+KE +RM  IAP AIPH++ ++T L G  V   T ++ N+ ++ H+  +W + 
Sbjct: 340 LKYLQGVVKETMRMKPIAPLAIPHKTAKETTLMGTKVAKGTRIMVNLYALHHNQNIWPDP 399

Query: 367 EHELDKFKAQRFMKMAESKGKERGANPMCENISFGAGRRQCAGMELANMLVPLTLANLLK 188
                KF  +RF++ A      +        + F AG R CAGM+L  +     LANL+ 
Sbjct: 400 Y----KFMPERFLEGATGTAYNKAMEQ--SFLPFSAGMRICAGMDLGKLQFAFALANLVN 453

Query: 187 AFEWTC--ETSPPDLTPYCPFMLRSLKKPLRARIFHR 83
           AF+W+C  E   PD+     F+L  +K PL ARI  R
Sbjct: 454 AFKWSCVEEGKLPDMGEELSFVL-LMKTPLEARIAGR 489


>sp|Q948Y1.1|C719A_COPJA RecName: Full=(S)-canadine synthase; AltName:
           Full=(S)-tetrahydrocolumbamine oxidase
           (methylenedioxy-bridge-forming); AltName:
           Full=Cytochrome P450 719A1 gi|15822820|dbj|BAB68769.1|
           methylenedioxy bridge-forming enzyme [Coptis japonica]
          Length = 491

 Score =  141 bits (355), Expect = 3e-31
 Identities = 84/221 (38%), Positives = 124/221 (56%), Gaps = 6/221 (2%)
 Frame = -2

Query: 727 EEIAFNVFEIFFLGVDSTATSMEWALAYFVAHPEMQNKAFNEIQTVTPSKKDVAMEDVSK 548
           E I F +FE++ LGVDST+++  WALAY +     Q K + +I+        V +EDV+K
Sbjct: 280 EVIIFCIFELYLLGVDSTSSTTTWALAYLIREQGAQEKLYQDIRMTLGDVDLVKIEDVNK 339

Query: 547 LTYLEAVLKEVLRMHAIAPFAIPHRSTRKTILGGFVVPANTTVLFNIDSVAHDPKLWGED 368
           L YL+ V+KE +RM  IAP AIPH++ ++T L G  V   T ++ N+ ++ H+  +W + 
Sbjct: 340 LKYLQGVVKETMRMKPIAPLAIPHKTAKETTLMGTKVAKGTRIMVNLYALHHNQNIWPDP 399

Query: 367 EHELDKFKAQRFMK----MAESKGKERGANPMCENISFGAGRRQCAGMELANMLVPLTLA 200
                KF  +RF++     A +K  E+   P      F AG R CAGM+L  +     LA
Sbjct: 400 Y----KFMPERFLEGETGTAYNKAMEQSFLP------FSAGMRICAGMDLGKLQFAFALA 449

Query: 199 NLLKAFEWTC--ETSPPDLTPYCPFMLRSLKKPLRARIFHR 83
           NL+ AF+W+C  E   PD+     F+L  +K PL ARI  R
Sbjct: 450 NLVNAFKWSCVEEGKLPDMGEELSFVL-LMKTPLEARIAGR 489


>sp|Q50EK4.1|C75A1_PINTA RecName: Full=Cytochrome P450 750A1; AltName: Full=Cytochrome P450
           CYPC gi|59800268|gb|AAX07433.1| cytochrome P450 CYPC
           [Pinus taeda]
          Length = 525

 Score =  140 bits (352), Expect = 6e-31
 Identities = 70/195 (35%), Positives = 106/195 (54%)
 Frame = -2

Query: 733 TNEEIAFNVFEIFFLGVDSTATSMEWALAYFVAHPEMQNKAFNEIQTVTPSKKDVAMEDV 554
           T E I    +E+F  G++++A  +EWA++  + HP    K   EI++V   +  V   D+
Sbjct: 306 TREIIKAITYELFSAGMETSANVLEWAMSELLRHPHAMKKLQQEIESVVGQQGTVKESDL 365

Query: 553 SKLTYLEAVLKEVLRMHAIAPFAIPHRSTRKTILGGFVVPANTTVLFNIDSVAHDPKLWG 374
           + + YL  V+KE LR++   P A+PH S     +GG+ +P  T V+ N+ ++  DP +WG
Sbjct: 366 ASIVYLHCVVKETLRLYPSLPLALPHESLEAVTVGGYYIPKKTMVIMNLWAIGRDPSVWG 425

Query: 373 EDEHELDKFKAQRFMKMAESKGKERGANPMCENISFGAGRRQCAGMELANMLVPLTLANL 194
            D  E   FK +RFM+M E+     G       + FGAGRR C G  +A + V  TLA L
Sbjct: 426 ADASE---FKPERFMQMEENGIDLSGGQSDFRMLPFGAGRRTCPGSAMAILTVEFTLAQL 482

Query: 193 LKAFEWTCETSPPDL 149
           L  F+W  E  P +L
Sbjct: 483 LHTFDWRVEGDPSEL 497


>ref|XP_002978564.1| hypothetical protein SELMODRAFT_108700 [Selaginella moellendorffii]
           gi|300153913|gb|EFJ20550.1| hypothetical protein
           SELMODRAFT_108700 [Selaginella moellendorffii]
          Length = 502

 Score =  139 bits (351), Expect = 7e-31
 Identities = 82/221 (37%), Positives = 124/221 (56%), Gaps = 2/221 (0%)
 Frame = -2

Query: 733 TNEEIAFNVFEIFFLGVDSTATSMEWALAYFVAHPEMQNKAFNEIQTVTPSKKDVAMEDV 554
           T+E++     E    G D+TA ++EW+LA  + HP +Q K   EI + +   K V  +D+
Sbjct: 288 TDEDLIVLCSEFLNAGADTTANTLEWSLANVIKHPAVQKKLLEEIHS-SVGDKPVTEKDI 346

Query: 553 SKLTYLEAVLKEVLRMHAIAPFAIPHRSTRKTILGGFVVPANTTVLFNIDSVAHDPKLWG 374
            KL YL+AV+KE LR H      +PH  T    LGG+ +P + T+LFNI ++ +DP+LW 
Sbjct: 347 DKLVYLKAVVKETLRKHPPGYTTLPHAVTEPCKLGGYDIPVHATLLFNIYAINNDPELWT 406

Query: 373 EDEHELDKFKAQRFMKMAESKGKERGANPMCENISFGAGRRQCAGMELANMLVPLTLANL 194
                 D++K +RF++   +      ++ +   I FGAGRR C G+ LA + V L LA L
Sbjct: 407 NP----DEYKPERFLEGPGASADFTASSGVLNLIPFGAGRRICPGLGLATLHVHLVLARL 462

Query: 193 LKAFEWTC--ETSPPDLTPYCPFMLRSLKKPLRARIFHRAL 77
           ++ FEW      +  DLTP   F +  +K+PLRA +  R L
Sbjct: 463 VQEFEWNTVPGDTAVDLTPIQEFTV-VMKQPLRATLKARRL 502


>ref|XP_002970487.1| hypothetical protein SELMODRAFT_93385 [Selaginella moellendorffii]
           gi|300162003|gb|EFJ28617.1| hypothetical protein
           SELMODRAFT_93385 [Selaginella moellendorffii]
          Length = 503

 Score =  139 bits (350), Expect = 1e-30
 Identities = 84/222 (37%), Positives = 125/222 (56%), Gaps = 3/222 (1%)
 Frame = -2

Query: 733 TNEEIAFNVFEIFFLGVDSTATSMEWALAYFVAHPEMQNKAFNEIQTVTPSKKDVAMEDV 554
           T+E++     E    G D+TA ++EW+LA  + HP +Q K   EI + +   K V  +D+
Sbjct: 289 TDEDLIVLCSEFLNAGADTTANTLEWSLANVIKHPAVQKKLLEEIHS-SVGDKPVTEKDI 347

Query: 553 SKLTYLEAVLKEVLRMHAIAPFAIPHRSTRKTILGGFVVPANTTVLFNIDSVAHDPKLWG 374
            KL YL+AV+KE LR H      +PH  T    LGG+ +P + T+LFNI ++ +DP+LW 
Sbjct: 348 DKLVYLKAVVKETLRKHPPGYTTLPHAVTEPCKLGGYDIPVHATLLFNIYAINNDPELWT 407

Query: 373 EDEHELDKFKAQRFMKMAESKGKERGANPMCENISFGAGRRQCAGMELANMLVPLTLANL 194
                 D++K +RF++   +      ++     I FGAGRR C G+ LA + V L LA L
Sbjct: 408 NP----DEYKPERFLEGPGASADFTASSGALNLIPFGAGRRICPGLGLATLHVHLVLARL 463

Query: 193 LKAFEWTC---ETSPPDLTPYCPFMLRSLKKPLRARIFHRAL 77
           ++ FEW     ET+  DLTP   F +  +K+PLRA +  R L
Sbjct: 464 VQEFEWNTVPGETA-VDLTPIQEFTV-VMKQPLRATLKARRL 503


>dbj|BAG75116.1| cytochrome P450 [Eschscholzia californica]
          Length = 495

 Score =  139 bits (350), Expect = 1e-30
 Identities = 84/223 (37%), Positives = 126/223 (56%), Gaps = 8/223 (3%)
 Frame = -2

Query: 727 EEIAFNVFEIFFLGVDSTATSMEWALAYFVAHPEMQNKAFNEIQTVTP--SKKDVAMEDV 554
           E + F +FE + LGVDST+++  WALAY V  P +Q++ +NE+        +K + +E +
Sbjct: 283 EMVIFAIFETYLLGVDSTSSTTAWALAYLVREPNVQDRLYNELNNYANYNDRKILKVEVI 342

Query: 553 SKLTYLEAVLKEVLRMHAIAPFAIPHRSTRKTILGGFVVPANTTVLFNIDSVAHDPKLWG 374
           +KL YL+AV KE +RM  IAP AIPH++ R T L G  +   T V+ N+ ++ H+  ++ 
Sbjct: 343 NKLQYLQAVTKETMRMKPIAPLAIPHKACRDTSLMGTKIDKGTKVMVNLHALHHNENVFT 402

Query: 373 EDEHELDKFKAQRFMKM----AESKGKERGANPMCENISFGAGRRQCAGMELANMLVPLT 206
           +      KF  +RF+K+       K  E+   P      F AG R CAGMEL  +     
Sbjct: 403 DP----FKFMPERFLKVNNEDVNMKAMEQSLLP------FSAGMRICAGMELGKLQFSFA 452

Query: 205 LANLLKAFEWTC--ETSPPDLTPYCPFMLRSLKKPLRARIFHR 83
           LANL+ AF+W+C  +   PD++    F+L  +K PL ARI  R
Sbjct: 453 LANLVYAFKWSCVDDGVLPDMSDELGFVL-FMKTPLEARIVRR 494