BLASTX nr result
ID: Ephedra28_contig00012902
seq
BLASTX 2.2.25 [Feb-01-2011] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= Ephedra28_contig00012902 (556 letters) Database: ./nr 37,332,560 sequences; 13,225,080,153 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|XP_006845407.1| hypothetical protein AMTR_s00019p00072350 [A... 181 8e-44 gb|EOY29291.1| Monogalactosyl diacylglycerol synthase 1 [Theobro... 177 2e-42 ref|XP_004498820.1| PREDICTED: probable monogalactosyldiacylglyc... 176 3e-42 ref|XP_004159249.1| PREDICTED: LOW QUALITY PROTEIN: monogalactos... 175 8e-42 ref|XP_004138694.1| PREDICTED: monogalactosyldiacylglycerol synt... 175 8e-42 sp|P93115.1|MGDG_CUCSA RecName: Full=Monogalactosyldiacylglycero... 175 8e-42 ref|XP_002515397.1| 1,2-diacylglycerol 3-beta-galactosyltransfer... 175 8e-42 ref|XP_002309689.1| MGDG synthase type A family protein [Populus... 175 8e-42 ref|XP_004956860.1| PREDICTED: probable monogalactosyldiacylglyc... 174 1e-41 sp|Q69QJ7.1|MGDG1_ORYSJ RecName: Full=Probable monogalactosyldia... 173 2e-41 ref|NP_001063213.2| Os09g0423600 [Oryza sativa Japonica Group] g... 173 2e-41 gb|EEC84596.1| hypothetical protein OsI_31415 [Oryza sativa Indi... 173 2e-41 dbj|BAN66663.1| monogalactosyldiacylglycerol synthase 1 type A [... 172 4e-41 gb|EXB60094.1| putative monogalactosyldiacylglycerol synthase [M... 172 5e-41 gb|ACF84568.1| unknown [Zea mays] 172 5e-41 ref|NP_001142118.1| opaque endosperm5 [Zea mays] gi|194690888|gb... 172 5e-41 ref|XP_006660638.1| PREDICTED: probable monogalactosyldiacylglyc... 172 6e-41 ref|XP_006450252.1| hypothetical protein CICLE_v10007936mg [Citr... 172 6e-41 gb|EMJ23504.1| hypothetical protein PRUPE_ppa003927mg [Prunus pe... 172 6e-41 ref|XP_002324925.2| MGDG synthase type A family protein [Populus... 171 8e-41 >ref|XP_006845407.1| hypothetical protein AMTR_s00019p00072350 [Amborella trichopoda] gi|548847979|gb|ERN07082.1| hypothetical protein AMTR_s00019p00072350 [Amborella trichopoda] Length = 518 Score = 181 bits (460), Expect = 8e-44 Identities = 88/99 (88%), Positives = 93/99 (93%) Frame = +1 Query: 1 DCIITKAGPGTIAEAMIRGLPVILNDYIAGQEAGNVPFVVDNGAGKFSKSPKEIANIVAD 180 DCIITKAGPGTIAEAMIRGLP+ILNDYIAGQEAGNVP+VV+NGAGKFSKSPKEIANIVA Sbjct: 410 DCIITKAGPGTIAEAMIRGLPIILNDYIAGQEAGNVPYVVENGAGKFSKSPKEIANIVAQ 469 Query: 181 WFGPKIDELKRFSQNALKMARPDAVFKIVHDLDELVNRR 297 WFGPK DELK SQNALK+ARPDAVFKIVHDL ELV +R Sbjct: 470 WFGPKADELKSMSQNALKLARPDAVFKIVHDLHELVRQR 508 >gb|EOY29291.1| Monogalactosyl diacylglycerol synthase 1 [Theobroma cacao] Length = 531 Score = 177 bits (449), Expect = 2e-42 Identities = 85/100 (85%), Positives = 92/100 (92%) Frame = +1 Query: 1 DCIITKAGPGTIAEAMIRGLPVILNDYIAGQEAGNVPFVVDNGAGKFSKSPKEIANIVAD 180 DCIITKAGPGTIAEAMIRGLP+ILNDYIAGQE GNVP+VV+NG GKFSKSPKEIANIVA Sbjct: 423 DCIITKAGPGTIAEAMIRGLPIILNDYIAGQEVGNVPYVVENGCGKFSKSPKEIANIVAQ 482 Query: 181 WFGPKIDELKRFSQNALKMARPDAVFKIVHDLDELVNRRD 300 WFGPK DELK SQNALK+ARP+AVFKIVHDL ELV +R+ Sbjct: 483 WFGPKADELKAMSQNALKLARPEAVFKIVHDLHELVRQRN 522 >ref|XP_004498820.1| PREDICTED: probable monogalactosyldiacylglycerol synthase, chloroplastic-like [Cicer arietinum] Length = 533 Score = 176 bits (447), Expect = 3e-42 Identities = 85/104 (81%), Positives = 94/104 (90%) Frame = +1 Query: 1 DCIITKAGPGTIAEAMIRGLPVILNDYIAGQEAGNVPFVVDNGAGKFSKSPKEIANIVAD 180 DCIITKAGPGTIAEAMIRGLP+ILNDYIAGQEAGNVP+VV+NG GK+SKSPKEIA IVAD Sbjct: 425 DCIITKAGPGTIAEAMIRGLPIILNDYIAGQEAGNVPYVVENGCGKYSKSPKEIATIVAD 484 Query: 181 WFGPKIDELKRFSQNALKMARPDAVFKIVHDLDELVNRRDEMLN 312 WFGPK DELK SQNAL+ ARPDAVFKIVHDL +LV +R +L+ Sbjct: 485 WFGPKADELKTMSQNALRQARPDAVFKIVHDLHDLVRQRRLLLD 528 >ref|XP_004159249.1| PREDICTED: LOW QUALITY PROTEIN: monogalactosyldiacylglycerol synthase, chloroplastic-like [Cucumis sativus] Length = 525 Score = 175 bits (443), Expect = 8e-42 Identities = 85/99 (85%), Positives = 91/99 (91%) Frame = +1 Query: 1 DCIITKAGPGTIAEAMIRGLPVILNDYIAGQEAGNVPFVVDNGAGKFSKSPKEIANIVAD 180 DCIITKAGPGTIAEAMIRGLP+ILNDYIAGQEAGNVP+VV+NG GKFSKSPKEIANIVA Sbjct: 419 DCIITKAGPGTIAEAMIRGLPIILNDYIAGQEAGNVPYVVENGCGKFSKSPKEIANIVAK 478 Query: 181 WFGPKIDELKRFSQNALKMARPDAVFKIVHDLDELVNRR 297 WFGPK DEL SQNAL++ARPDAVFKIVHDL ELV +R Sbjct: 479 WFGPKADELLIMSQNALRLARPDAVFKIVHDLHELVKQR 517 >ref|XP_004138694.1| PREDICTED: monogalactosyldiacylglycerol synthase, chloroplastic-like [Cucumis sativus] Length = 525 Score = 175 bits (443), Expect = 8e-42 Identities = 85/99 (85%), Positives = 91/99 (91%) Frame = +1 Query: 1 DCIITKAGPGTIAEAMIRGLPVILNDYIAGQEAGNVPFVVDNGAGKFSKSPKEIANIVAD 180 DCIITKAGPGTIAEAMIRGLP+ILNDYIAGQEAGNVP+VV+NG GKFSKSPKEIANIVA Sbjct: 419 DCIITKAGPGTIAEAMIRGLPIILNDYIAGQEAGNVPYVVENGCGKFSKSPKEIANIVAK 478 Query: 181 WFGPKIDELKRFSQNALKMARPDAVFKIVHDLDELVNRR 297 WFGPK DEL SQNAL++ARPDAVFKIVHDL ELV +R Sbjct: 479 WFGPKADELLIMSQNALRLARPDAVFKIVHDLHELVKQR 517 >sp|P93115.1|MGDG_CUCSA RecName: Full=Monogalactosyldiacylglycerol synthase, chloroplastic; Short=CsMGD1; Flags: Precursor gi|1805254|gb|AAC49624.1| monogalactosyldiacylglycerol synthase [Cucumis sativus] Length = 525 Score = 175 bits (443), Expect = 8e-42 Identities = 85/99 (85%), Positives = 91/99 (91%) Frame = +1 Query: 1 DCIITKAGPGTIAEAMIRGLPVILNDYIAGQEAGNVPFVVDNGAGKFSKSPKEIANIVAD 180 DCIITKAGPGTIAEAMIRGLP+ILNDYIAGQEAGNVP+VV+NG GKFSKSPKEIANIVA Sbjct: 419 DCIITKAGPGTIAEAMIRGLPIILNDYIAGQEAGNVPYVVENGCGKFSKSPKEIANIVAK 478 Query: 181 WFGPKIDELKRFSQNALKMARPDAVFKIVHDLDELVNRR 297 WFGPK DEL SQNAL++ARPDAVFKIVHDL ELV +R Sbjct: 479 WFGPKADELLIMSQNALRLARPDAVFKIVHDLHELVKQR 517 >ref|XP_002515397.1| 1,2-diacylglycerol 3-beta-galactosyltransferase, putative [Ricinus communis] gi|223545341|gb|EEF46846.1| 1,2-diacylglycerol 3-beta-galactosyltransferase, putative [Ricinus communis] Length = 535 Score = 175 bits (443), Expect = 8e-42 Identities = 84/100 (84%), Positives = 91/100 (91%) Frame = +1 Query: 1 DCIITKAGPGTIAEAMIRGLPVILNDYIAGQEAGNVPFVVDNGAGKFSKSPKEIANIVAD 180 DCIITKAGPGTIAEAMIRGLP+ILN YIAGQE GNVP+VV+N GKFSKSPKEIANIV + Sbjct: 427 DCIITKAGPGTIAEAMIRGLPIILNGYIAGQEVGNVPYVVENKCGKFSKSPKEIANIVGE 486 Query: 181 WFGPKIDELKRFSQNALKMARPDAVFKIVHDLDELVNRRD 300 WFGPK DELK SQNALK+ARPDAVFKIVHDLDELV +R+ Sbjct: 487 WFGPKADELKAMSQNALKLARPDAVFKIVHDLDELVRQRN 526 >ref|XP_002309689.1| MGDG synthase type A family protein [Populus trichocarpa] gi|222855665|gb|EEE93212.1| MGDG synthase type A family protein [Populus trichocarpa] Length = 537 Score = 175 bits (443), Expect = 8e-42 Identities = 84/100 (84%), Positives = 90/100 (90%) Frame = +1 Query: 1 DCIITKAGPGTIAEAMIRGLPVILNDYIAGQEAGNVPFVVDNGAGKFSKSPKEIANIVAD 180 DCIITKAGPGTIAEAMIRGLP+ILNDYIAGQE GNVP+VV+NG GKFSKSPKEIA IVA+ Sbjct: 429 DCIITKAGPGTIAEAMIRGLPIILNDYIAGQEVGNVPYVVENGCGKFSKSPKEIAKIVAE 488 Query: 181 WFGPKIDELKRFSQNALKMARPDAVFKIVHDLDELVNRRD 300 WFGPK DEL SQNALK+ARPDAVFKIVHDL ELV R+ Sbjct: 489 WFGPKADELSAMSQNALKLARPDAVFKIVHDLHELVRHRN 528 >ref|XP_004956860.1| PREDICTED: probable monogalactosyldiacylglycerol synthase 1, chloroplastic-like [Setaria italica] Length = 531 Score = 174 bits (442), Expect = 1e-41 Identities = 83/99 (83%), Positives = 91/99 (91%) Frame = +1 Query: 1 DCIITKAGPGTIAEAMIRGLPVILNDYIAGQEAGNVPFVVDNGAGKFSKSPKEIANIVAD 180 DCIITKAGPGTIAEAMIRGLP+ILNDYIAGQEAGNVP+VV+NG GKFSKSPK+IANIVAD Sbjct: 423 DCIITKAGPGTIAEAMIRGLPIILNDYIAGQEAGNVPYVVENGCGKFSKSPKQIANIVAD 482 Query: 181 WFGPKIDELKRFSQNALKMARPDAVFKIVHDLDELVNRR 297 WFGPK DE + SQN LK+ARPDAVFKIVHDL ELV ++ Sbjct: 483 WFGPKSDEFRIMSQNCLKLARPDAVFKIVHDLHELVRQK 521 >sp|Q69QJ7.1|MGDG1_ORYSJ RecName: Full=Probable monogalactosyldiacylglycerol synthase 1, chloroplastic; Short=OsMGD1; Flags: Precursor gi|50725897|dbj|BAD33425.1| putative MGDG synthase type A [Oryza sativa Japonica Group] gi|215769330|dbj|BAH01559.1| unnamed protein product [Oryza sativa Japonica Group] gi|222641597|gb|EEE69729.1| hypothetical protein OsJ_29413 [Oryza sativa Japonica Group] Length = 535 Score = 173 bits (439), Expect = 2e-41 Identities = 83/99 (83%), Positives = 91/99 (91%) Frame = +1 Query: 1 DCIITKAGPGTIAEAMIRGLPVILNDYIAGQEAGNVPFVVDNGAGKFSKSPKEIANIVAD 180 DCIITKAGPGTIAEAMIRGLP+ILN YIAGQEAGNVP+VVDNG GKFSKSP++IA IVAD Sbjct: 427 DCIITKAGPGTIAEAMIRGLPIILNGYIAGQEAGNVPYVVDNGCGKFSKSPEQIAKIVAD 486 Query: 181 WFGPKIDELKRFSQNALKMARPDAVFKIVHDLDELVNRR 297 WFGP+ DELK SQNALK+ARPDAVFKIVHDL ELV ++ Sbjct: 487 WFGPRSDELKMMSQNALKLARPDAVFKIVHDLHELVRQK 525 >ref|NP_001063213.2| Os09g0423600 [Oryza sativa Japonica Group] gi|255678907|dbj|BAF25127.2| Os09g0423600 [Oryza sativa Japonica Group] Length = 191 Score = 173 bits (439), Expect = 2e-41 Identities = 83/99 (83%), Positives = 91/99 (91%) Frame = +1 Query: 1 DCIITKAGPGTIAEAMIRGLPVILNDYIAGQEAGNVPFVVDNGAGKFSKSPKEIANIVAD 180 DCIITKAGPGTIAEAMIRGLP+ILN YIAGQEAGNVP+VVDNG GKFSKSP++IA IVAD Sbjct: 83 DCIITKAGPGTIAEAMIRGLPIILNGYIAGQEAGNVPYVVDNGCGKFSKSPEQIAKIVAD 142 Query: 181 WFGPKIDELKRFSQNALKMARPDAVFKIVHDLDELVNRR 297 WFGP+ DELK SQNALK+ARPDAVFKIVHDL ELV ++ Sbjct: 143 WFGPRSDELKMMSQNALKLARPDAVFKIVHDLHELVRQK 181 >gb|EEC84596.1| hypothetical protein OsI_31415 [Oryza sativa Indica Group] Length = 324 Score = 173 bits (439), Expect = 2e-41 Identities = 83/99 (83%), Positives = 91/99 (91%) Frame = +1 Query: 1 DCIITKAGPGTIAEAMIRGLPVILNDYIAGQEAGNVPFVVDNGAGKFSKSPKEIANIVAD 180 DCIITKAGPGTIAEAMIRGLP+ILN YIAGQEAGNVP+VVDNG GKFSKSP++IA IVAD Sbjct: 216 DCIITKAGPGTIAEAMIRGLPIILNGYIAGQEAGNVPYVVDNGCGKFSKSPEQIAKIVAD 275 Query: 181 WFGPKIDELKRFSQNALKMARPDAVFKIVHDLDELVNRR 297 WFGP+ DELK SQNALK+ARPDAVFKIVHDL ELV ++ Sbjct: 276 WFGPRSDELKMMSQNALKLARPDAVFKIVHDLHELVRQK 314 >dbj|BAN66663.1| monogalactosyldiacylglycerol synthase 1 type A [Sesamum indicum] Length = 516 Score = 172 bits (437), Expect = 4e-41 Identities = 83/100 (83%), Positives = 91/100 (91%) Frame = +1 Query: 1 DCIITKAGPGTIAEAMIRGLPVILNDYIAGQEAGNVPFVVDNGAGKFSKSPKEIANIVAD 180 DCIITKAGPGTIAEAMIRGLP+ILNDYIAGQEAGNVP+VV NG GKFSKSPKEIANIV+ Sbjct: 409 DCIITKAGPGTIAEAMIRGLPIILNDYIAGQEAGNVPYVVQNGCGKFSKSPKEIANIVSQ 468 Query: 181 WFGPKIDELKRFSQNALKMARPDAVFKIVHDLDELVNRRD 300 WFGPK DEL SQN+L++ARPDAVFKIVHDL ELV +R+ Sbjct: 469 WFGPKQDELVAMSQNSLRLARPDAVFKIVHDLHELVRQRN 508 >gb|EXB60094.1| putative monogalactosyldiacylglycerol synthase [Morus notabilis] Length = 542 Score = 172 bits (436), Expect = 5e-41 Identities = 82/95 (86%), Positives = 88/95 (92%) Frame = +1 Query: 1 DCIITKAGPGTIAEAMIRGLPVILNDYIAGQEAGNVPFVVDNGAGKFSKSPKEIANIVAD 180 DCIITKAGPGTIAEAMIRG+P+ILNDYIAGQE GNVP+VV+NG GKFSKSPKEIANIVA Sbjct: 428 DCIITKAGPGTIAEAMIRGVPIILNDYIAGQEVGNVPYVVENGCGKFSKSPKEIANIVAQ 487 Query: 181 WFGPKIDELKRFSQNALKMARPDAVFKIVHDLDEL 285 WFGPK DELK SQNALK+A+PDAVFKIVHDL EL Sbjct: 488 WFGPKADELKAMSQNALKLAKPDAVFKIVHDLHEL 522 >gb|ACF84568.1| unknown [Zea mays] Length = 529 Score = 172 bits (436), Expect = 5e-41 Identities = 82/99 (82%), Positives = 90/99 (90%) Frame = +1 Query: 1 DCIITKAGPGTIAEAMIRGLPVILNDYIAGQEAGNVPFVVDNGAGKFSKSPKEIANIVAD 180 DCIITKAGPGTIAEAMIRGLP+ILNDYIAGQEAGNVP+VV+NG GKFSKSPK+IA IVAD Sbjct: 421 DCIITKAGPGTIAEAMIRGLPIILNDYIAGQEAGNVPYVVENGCGKFSKSPKQIAKIVAD 480 Query: 181 WFGPKIDELKRFSQNALKMARPDAVFKIVHDLDELVNRR 297 WFGP+ DE + SQNALK+ARPDAVFKIVHDL ELV + Sbjct: 481 WFGPRSDEFRVMSQNALKLARPDAVFKIVHDLHELVREK 519 >ref|NP_001142118.1| opaque endosperm5 [Zea mays] gi|194690888|gb|ACF79528.1| unknown [Zea mays] gi|194707194|gb|ACF87681.1| unknown [Zea mays] gi|195609976|gb|ACG26818.1| MGDG synthase type A [Zea mays] gi|414885523|tpg|DAA61537.1| TPA: opaque endosperm5 [Zea mays] Length = 529 Score = 172 bits (436), Expect = 5e-41 Identities = 82/99 (82%), Positives = 90/99 (90%) Frame = +1 Query: 1 DCIITKAGPGTIAEAMIRGLPVILNDYIAGQEAGNVPFVVDNGAGKFSKSPKEIANIVAD 180 DCIITKAGPGTIAEAMIRGLP+ILNDYIAGQEAGNVP+VV+NG GKFSKSPK+IA IVAD Sbjct: 421 DCIITKAGPGTIAEAMIRGLPIILNDYIAGQEAGNVPYVVENGCGKFSKSPKQIAKIVAD 480 Query: 181 WFGPKIDELKRFSQNALKMARPDAVFKIVHDLDELVNRR 297 WFGP+ DE + SQNALK+ARPDAVFKIVHDL ELV + Sbjct: 481 WFGPRSDEFRVMSQNALKLARPDAVFKIVHDLHELVREK 519 >ref|XP_006660638.1| PREDICTED: probable monogalactosyldiacylglycerol synthase 1, chloroplastic-like, partial [Oryza brachyantha] Length = 413 Score = 172 bits (435), Expect = 6e-41 Identities = 82/99 (82%), Positives = 91/99 (91%) Frame = +1 Query: 1 DCIITKAGPGTIAEAMIRGLPVILNDYIAGQEAGNVPFVVDNGAGKFSKSPKEIANIVAD 180 DCIITKAGPGTIAEAMIRGLP+ILN YIAGQEAGNVP+VV+NG GKFSKSP++IA IVAD Sbjct: 305 DCIITKAGPGTIAEAMIRGLPIILNGYIAGQEAGNVPYVVENGCGKFSKSPEQIAKIVAD 364 Query: 181 WFGPKIDELKRFSQNALKMARPDAVFKIVHDLDELVNRR 297 WFGP+ DELK SQNALK+ARPDAVFKIVHDL ELV ++ Sbjct: 365 WFGPRSDELKMMSQNALKLARPDAVFKIVHDLHELVRQK 403 >ref|XP_006450252.1| hypothetical protein CICLE_v10007936mg [Citrus clementina] gi|568860010|ref|XP_006483521.1| PREDICTED: monogalactosyldiacylglycerol synthase, chloroplastic-like [Citrus sinensis] gi|557553478|gb|ESR63492.1| hypothetical protein CICLE_v10007936mg [Citrus clementina] Length = 536 Score = 172 bits (435), Expect = 6e-41 Identities = 81/100 (81%), Positives = 93/100 (93%) Frame = +1 Query: 1 DCIITKAGPGTIAEAMIRGLPVILNDYIAGQEAGNVPFVVDNGAGKFSKSPKEIANIVAD 180 DCIITKAGPGTIAEAMIRGLP+ILND+IAGQEAGNVP+VV+NG GKFSKSPKEIAN+V+ Sbjct: 428 DCIITKAGPGTIAEAMIRGLPIILNDFIAGQEAGNVPYVVENGCGKFSKSPKEIANMVSQ 487 Query: 181 WFGPKIDELKRFSQNALKMARPDAVFKIVHDLDELVNRRD 300 WFGP++DELK SQNALK+ARPDAVF+IV DL ELV +R+ Sbjct: 488 WFGPRMDELKAMSQNALKLARPDAVFRIVQDLHELVRQRN 527 >gb|EMJ23504.1| hypothetical protein PRUPE_ppa003927mg [Prunus persica] Length = 539 Score = 172 bits (435), Expect = 6e-41 Identities = 81/99 (81%), Positives = 90/99 (90%) Frame = +1 Query: 1 DCIITKAGPGTIAEAMIRGLPVILNDYIAGQEAGNVPFVVDNGAGKFSKSPKEIANIVAD 180 DCIITKAGPGTIAEAMIRGLP+ILNDYIAGQE GNVP+VV+NG GK+SKSPKEIA IVA Sbjct: 431 DCIITKAGPGTIAEAMIRGLPIILNDYIAGQEVGNVPYVVENGCGKYSKSPKEIAKIVAQ 490 Query: 181 WFGPKIDELKRFSQNALKMARPDAVFKIVHDLDELVNRR 297 WFGPK DELK S+NA+K+ARPD+VFKIVHDL ELV +R Sbjct: 491 WFGPKADELKAMSENAIKLARPDSVFKIVHDLHELVRQR 529 >ref|XP_002324925.2| MGDG synthase type A family protein [Populus trichocarpa] gi|550317900|gb|EEF03490.2| MGDG synthase type A family protein [Populus trichocarpa] Length = 537 Score = 171 bits (434), Expect = 8e-41 Identities = 83/100 (83%), Positives = 90/100 (90%) Frame = +1 Query: 1 DCIITKAGPGTIAEAMIRGLPVILNDYIAGQEAGNVPFVVDNGAGKFSKSPKEIANIVAD 180 DCIITKAGPGTIAEAMIRGLP+ILNDYIAGQE GNVP VV+NG GKFSKSPKEIA IVA+ Sbjct: 429 DCIITKAGPGTIAEAMIRGLPIILNDYIAGQEVGNVPHVVENGCGKFSKSPKEIAKIVAE 488 Query: 181 WFGPKIDELKRFSQNALKMARPDAVFKIVHDLDELVNRRD 300 WFG K DELK SQNALK+ARPD+VFKIVHDL ELV +R+ Sbjct: 489 WFGSKADELKAMSQNALKLARPDSVFKIVHDLHELVRQRN 528