BLASTX nr result
ID: Ephedra28_contig00006187
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Ephedra28_contig00006187
(363 letters)
Database: ./nr
37,332,560 sequences; 13,225,080,153 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|Q50EK0.1|C16B2_PICSI RecName: Full=Cytochrome P450 716B2; Alt... 114 1e-23
sp|Q50EK1.1|C16B1_PICSI RecName: Full=Cytochrome P450 716B1; Alt... 114 1e-23
gb|ABR16707.1| unknown [Picea sitchensis] 104 1e-20
ref|XP_002965726.1| hypothetical protein SELMODRAFT_84634 [Selag... 96 5e-18
ref|NP_001268115.1| cytochrome P450 CYP716A15 [Vitis vinifera] g... 95 8e-18
sp|Q8W4T9.1|T13H_TAXCU RecName: Full=Taxane 13-alpha-hydroxylase... 94 2e-17
ref|XP_006340081.1| PREDICTED: cytochrome P450 716B1-like [Solan... 94 2e-17
ref|NP_001268076.1| cytochrome P450 CYP716A17 [Vitis vinifera] g... 94 2e-17
ref|XP_002527982.1| cytochrome P450, putative [Ricinus communis]... 94 2e-17
gb|ESW31548.1| hypothetical protein PHAVU_002G247300g [Phaseolus... 93 3e-17
ref|XP_004239296.1| PREDICTED: cytochrome P450 716B2-like [Solan... 93 3e-17
ref|XP_004228960.1| PREDICTED: cytochrome P450 716B1-like [Solan... 93 3e-17
emb|CAN72302.1| hypothetical protein VITISV_041935 [Vitis vinifera] 93 3e-17
ref|XP_006338129.1| PREDICTED: cytochrome P450 716B2-like [Solan... 93 4e-17
gb|ABC94482.1| putative taxane 13-alpha-hydroxylase cytochrome P... 93 4e-17
ref|XP_002266024.1| PREDICTED: cytochrome P450 716B2 [Vitis vini... 93 4e-17
gb|AAX20147.1| taxane 13-alpha-hydroxylase [Taxus x media] 92 7e-17
gb|AAX59903.1| 13-alpha-hydroxylase [Taxus wallichiana var. chin... 92 9e-17
emb|CBI39273.3| unnamed protein product [Vitis vinifera] 92 9e-17
ref|XP_002279608.1| PREDICTED: cytochrome P450 716B2 [Vitis vini... 92 9e-17
>sp|Q50EK0.1|C16B2_PICSI RecName: Full=Cytochrome P450 716B2; AltName: Full=Cytochrome P450
CYPA2 gi|59800276|gb|AAX07437.1| cytochrome P450 CYPA2
[Picea sitchensis]
Length = 497
Score = 114 bits (286), Expect = 1e-23
Identities = 57/98 (58%), Positives = 70/98 (71%), Gaps = 6/98 (6%)
Frame = -2
Query: 278 MIYTAILAVLL-CAFFL-----KRKASLKGTPPGSLGWPLLGETMEFLAQQRKGNPQEFV 117
+++T LA + AFF R + +G PPG+ GWPL+GET+EFL QRKGNP++F
Sbjct: 21 LMFTVFLASFIGLAFFFYLISNHRTKAWRGIPPGTFGWPLIGETLEFLGCQRKGNPRDFF 80
Query: 116 KSRAAKYGNVFKTSLIGNPTVVFGGAEGNRFLFSNENK 3
SR KYGNVF TSL+G+PTVVF EGNRFLFSNENK
Sbjct: 81 DSRTQKYGNVFTTSLVGHPTVVFCSPEGNRFLFSNENK 118
>sp|Q50EK1.1|C16B1_PICSI RecName: Full=Cytochrome P450 716B1; AltName: Full=Cytochrome P450
CYPA1 gi|59800274|gb|AAX07436.1| cytochrome P450 CYPA1
[Picea sitchensis]
Length = 493
Score = 114 bits (286), Expect = 1e-23
Identities = 57/98 (58%), Positives = 70/98 (71%), Gaps = 6/98 (6%)
Frame = -2
Query: 278 MIYTAILAVLL-CAFFL-----KRKASLKGTPPGSLGWPLLGETMEFLAQQRKGNPQEFV 117
+++T LA + AFF R + +G PPG+ GWPL+GET+EFL QRKGNP++F
Sbjct: 21 LMFTVFLASFIGLAFFFYLISNHRTKAWRGIPPGTFGWPLIGETLEFLGCQRKGNPRDFF 80
Query: 116 KSRAAKYGNVFKTSLIGNPTVVFGGAEGNRFLFSNENK 3
SR KYGNVF TSL+G+PTVVF EGNRFLFSNENK
Sbjct: 81 DSRTQKYGNVFTTSLVGHPTVVFCSPEGNRFLFSNENK 118
>gb|ABR16707.1| unknown [Picea sitchensis]
Length = 490
Score = 104 bits (260), Expect = 1e-20
Identities = 48/87 (55%), Positives = 60/87 (68%)
Frame = -2
Query: 263 ILAVLLCAFFLKRKASLKGTPPGSLGWPLLGETMEFLAQQRKGNPQEFVKSRAAKYGNVF 84
I A R +G PPG+ GWPL+GET+EFL Q++G+P +F SR +YGNVF
Sbjct: 30 IFLFFFSAIIRHRNRVSRGIPPGTFGWPLIGETVEFLRCQKRGSPHQFFDSRTQRYGNVF 89
Query: 83 KTSLIGNPTVVFGGAEGNRFLFSNENK 3
T L+G+PTVVF G EGNRFLF+NENK
Sbjct: 90 TTFLLGHPTVVFCGPEGNRFLFANENK 116
>ref|XP_002965726.1| hypothetical protein SELMODRAFT_84634 [Selaginella moellendorffii]
gi|300166540|gb|EFJ33146.1| hypothetical protein
SELMODRAFT_84634 [Selaginella moellendorffii]
Length = 469
Score = 95.9 bits (237), Expect = 5e-18
Identities = 45/94 (47%), Positives = 61/94 (64%)
Frame = -2
Query: 284 MNMIYTAILAVLLCAFFLKRKASLKGTPPGSLGWPLLGETMEFLAQQRKGNPQEFVKSRA 105
M ++ A +L+ F R + PPG+LGWP++GET++FLA RK F R
Sbjct: 1 MILLLGACFLLLITTIFAIRFSPSSNLPPGNLGWPIVGETLQFLALFRKNKAYSFFHERM 60
Query: 104 AKYGNVFKTSLIGNPTVVFGGAEGNRFLFSNENK 3
AK+G VFKTSL+G+PT+V G +GN+FLFS ENK
Sbjct: 61 AKHGGVFKTSLLGSPTIVMPGPDGNKFLFSQENK 94
>ref|NP_001268115.1| cytochrome P450 CYP716A15 [Vitis vinifera]
gi|326324797|dbj|BAJ84106.1| cytochrome P450 [Vitis
vinifera]
Length = 480
Score = 95.1 bits (235), Expect = 8e-18
Identities = 45/95 (47%), Positives = 66/95 (69%), Gaps = 3/95 (3%)
Frame = -2
Query: 278 MIYTAILAVLLCAFFLKRKASLKGT--PPGSLGWPLLGETMEFLAQQRKGNPQEFVKSRA 105
+I+ +++ L F K ++ G PPG +GWP++GE++EFL+ KG+P++F+ R
Sbjct: 10 LIFVLSVSIGLHLLFYKHRSHFTGPNLPPGKIGWPMVGESLEFLSTGWKGHPEKFIFDRI 69
Query: 104 AKYGN-VFKTSLIGNPTVVFGGAEGNRFLFSNENK 3
+KY + VFKTSL+G P VF GA GN+FLFSNENK
Sbjct: 70 SKYSSEVFKTSLLGEPAAVFAGAAGNKFLFSNENK 104
>sp|Q8W4T9.1|T13H_TAXCU RecName: Full=Taxane 13-alpha-hydroxylase; AltName: Full=Cytochrome
P450 725A2 gi|17148242|gb|AAL23619.1| taxane
13-alpha-hydroxylase [Taxus cuspidata]
Length = 485
Score = 93.6 bits (231), Expect = 2e-17
Identities = 44/88 (50%), Positives = 65/88 (73%)
Frame = -2
Query: 266 AILAVLLCAFFLKRKASLKGTPPGSLGWPLLGETMEFLAQQRKGNPQEFVKSRAAKYGNV 87
A++A ++ F KR +S+K PPG+LG+PL+GET++F+ PQ+F++ R +K+G+V
Sbjct: 19 AVMAGIILFFRSKRHSSVK-LPPGNLGFPLVGETLQFVRSLGSSTPQQFIEERMSKFGDV 77
Query: 86 FKTSLIGNPTVVFGGAEGNRFLFSNENK 3
FKTS+IG+PTVV G GNR + SNENK
Sbjct: 78 FKTSIIGHPTVVLCGPAGNRLVLSNENK 105
>ref|XP_006340081.1| PREDICTED: cytochrome P450 716B1-like [Solanum tuberosum]
Length = 474
Score = 93.6 bits (231), Expect = 2e-17
Identities = 46/87 (52%), Positives = 59/87 (67%), Gaps = 1/87 (1%)
Frame = -2
Query: 260 LAVLLCAFFLKRKASLKGTPPGSLGWPLLGETMEFLAQQRKGNPQEFVKSRAAKY-GNVF 84
LA+ + + R+ L PPGS GWP LGET++FL ++G P++FVK R KY +F
Sbjct: 12 LALFIIIVLVMRRKRLN-YPPGSCGWPFLGETLKFLKANKEGKPEKFVKERIEKYKSKIF 70
Query: 83 KTSLIGNPTVVFGGAEGNRFLFSNENK 3
KTSL+G VV GGA GN+FLFSNENK
Sbjct: 71 KTSLMGETVVVLGGASGNKFLFSNENK 97
>ref|NP_001268076.1| cytochrome P450 CYP716A17 [Vitis vinifera]
gi|326324799|dbj|BAJ84107.1| Cytochrome P450 [Vitis
vinifera]
Length = 480
Score = 93.6 bits (231), Expect = 2e-17
Identities = 45/99 (45%), Positives = 70/99 (70%), Gaps = 5/99 (5%)
Frame = -2
Query: 284 MNMIYTAILAVLLCAFFL--KRKASLKGT--PPGSLGWPLLGETMEFLAQQRKGNPQEFV 117
++++ ++L+V + + L K ++ G PPG +GWP++GE++EFL+ KG+P++F+
Sbjct: 6 LSLLLISVLSVSIRLYLLLYKHRSHFTGPNLPPGKIGWPMVGESLEFLSTGWKGHPEKFI 65
Query: 116 KSRAAKYGN-VFKTSLIGNPTVVFGGAEGNRFLFSNENK 3
R +KY + VFKTSL+G P VF GA GN+FLFSNENK
Sbjct: 66 FDRISKYSSEVFKTSLLGEPAAVFAGAAGNKFLFSNENK 104
>ref|XP_002527982.1| cytochrome P450, putative [Ricinus communis]
gi|223532608|gb|EEF34394.1| cytochrome P450, putative
[Ricinus communis]
Length = 480
Score = 93.6 bits (231), Expect = 2e-17
Identities = 45/102 (44%), Positives = 69/102 (67%), Gaps = 9/102 (8%)
Frame = -2
Query: 281 NMIYTAILAVLLCAFFLKRKA--------SLKGTPPGSLGWPLLGETMEFLAQQRKGNPQ 126
++ +AI ++ L +FL K+ SL TPPG+ GWPL+GET+E+L+ + G P+
Sbjct: 6 SVFISAIFSIALVLYFLNYKSKPRKDRAESLIPTPPGTTGWPLIGETLEYLSTTKAGIPE 65
Query: 125 EFVKSRAAKYGN-VFKTSLIGNPTVVFGGAEGNRFLFSNENK 3
+F+ R +Y + +FKTSL+G PTV+ A+GN+F+FSNENK
Sbjct: 66 KFINDRRKRYSSKLFKTSLLGQPTVLLCNADGNKFIFSNENK 107
>gb|ESW31548.1| hypothetical protein PHAVU_002G247300g [Phaseolus vulgaris]
Length = 478
Score = 93.2 bits (230), Expect = 3e-17
Identities = 46/98 (46%), Positives = 61/98 (62%), Gaps = 3/98 (3%)
Frame = -2
Query: 287 NMNMIYTAILAVLLCAFFLKRKASLKGT--PPGSLGWPLLGETMEFLAQQRKGNPQEFVK 114
NM ++ + +LC F+ R+ L+ PPG LGWP++GET EF+ +GN F+K
Sbjct: 5 NMVVLSAVLAFFVLCLHFITRRVRLRNLNLPPGRLGWPIVGETFEFMHAMLEGNVLRFLK 64
Query: 113 SRAAKY-GNVFKTSLIGNPTVVFGGAEGNRFLFSNENK 3
R KY VFKTS+ G+P VF G GN+FLFSNENK
Sbjct: 65 ERVEKYDSRVFKTSMFGDPVAVFCGPAGNKFLFSNENK 102
>ref|XP_004239296.1| PREDICTED: cytochrome P450 716B2-like [Solanum lycopersicum]
Length = 476
Score = 93.2 bits (230), Expect = 3e-17
Identities = 46/98 (46%), Positives = 64/98 (65%), Gaps = 4/98 (4%)
Frame = -2
Query: 284 MNMIYTAILAV---LLCAFFLKRKASLKGTPPGSLGWPLLGETMEFLAQQRKGNPQEFVK 114
M ++Y ++ V L+ L +K S +G PPG GWP+ GE++EFL+ KG+P++F+
Sbjct: 1 MELLYVCLVCVFVFLVSLLLLYKKKSGEGLPPGKTGWPVFGESLEFLSSGWKGHPEKFIF 60
Query: 113 SRAAKY-GNVFKTSLIGNPTVVFGGAEGNRFLFSNENK 3
R AKY +VFKT L+G VF GA N+FLFSNENK
Sbjct: 61 DRVAKYSSSVFKTHLLGEEAAVFCGASANKFLFSNENK 98
>ref|XP_004228960.1| PREDICTED: cytochrome P450 716B1-like [Solanum lycopersicum]
Length = 321
Score = 93.2 bits (230), Expect = 3e-17
Identities = 46/87 (52%), Positives = 59/87 (67%), Gaps = 1/87 (1%)
Frame = -2
Query: 260 LAVLLCAFFLKRKASLKGTPPGSLGWPLLGETMEFLAQQRKGNPQEFVKSRAAKY-GNVF 84
LA+ + + R+ L PPGS GWP LGET++FL ++G P++FVK R KY +F
Sbjct: 12 LALFIIIVIVMRRKRLN-YPPGSCGWPYLGETLDFLNANKEGKPEKFVKERIEKYKSKIF 70
Query: 83 KTSLIGNPTVVFGGAEGNRFLFSNENK 3
KTSL+G VV GGA GN+FLFSNENK
Sbjct: 71 KTSLMGETVVVLGGASGNKFLFSNENK 97
>emb|CAN72302.1| hypothetical protein VITISV_041935 [Vitis vinifera]
Length = 480
Score = 93.2 bits (230), Expect = 3e-17
Identities = 45/99 (45%), Positives = 69/99 (69%), Gaps = 5/99 (5%)
Frame = -2
Query: 284 MNMIYTAILAVLLCAFFL--KRKASLKGT--PPGSLGWPLLGETMEFLAQQRKGNPQEFV 117
++++ +L+V + + L K ++ G PPG +GWP++GE++EFL+ KG+P++F+
Sbjct: 6 LSLLLICVLSVSIRLYLLLYKHRSHFTGPNLPPGKIGWPMVGESLEFLSTGWKGHPEKFI 65
Query: 116 KSRAAKYGN-VFKTSLIGNPTVVFGGAEGNRFLFSNENK 3
R +KY + VFKTSL+G P VF GA GN+FLFSNENK
Sbjct: 66 FDRISKYSSEVFKTSLLGEPAAVFAGAAGNKFLFSNENK 104
>ref|XP_006338129.1| PREDICTED: cytochrome P450 716B2-like [Solanum tuberosum]
Length = 476
Score = 92.8 bits (229), Expect = 4e-17
Identities = 46/98 (46%), Positives = 64/98 (65%), Gaps = 4/98 (4%)
Frame = -2
Query: 284 MNMIYTAILAV---LLCAFFLKRKASLKGTPPGSLGWPLLGETMEFLAQQRKGNPQEFVK 114
M +Y +++ V L+ L + S +G PPG GWP+ GE++EFL+ KG+P++F+
Sbjct: 1 MEFLYVSLVCVFVFLVSLLLLYKNKSGEGLPPGKTGWPVFGESLEFLSTGWKGHPEKFIF 60
Query: 113 SRAAKY-GNVFKTSLIGNPTVVFGGAEGNRFLFSNENK 3
R AKY +VFKT L+G VF GA GN+FLFSNENK
Sbjct: 61 DRVAKYSSSVFKTHLLGEEAAVFCGASGNKFLFSNENK 98
>gb|ABC94482.1| putative taxane 13-alpha-hydroxylase cytochrome P450 [Artemisia
annua]
Length = 480
Score = 92.8 bits (229), Expect = 4e-17
Identities = 44/96 (45%), Positives = 61/96 (63%), Gaps = 4/96 (4%)
Frame = -2
Query: 278 MIYTAILAVLLCAFFLKRKASL--KGTPPGSLGWPLLGETMEFLAQQRKGNPQEFVKSRA 105
+++ +L + +C F + R S K PPGS GWP +GET+ ++ +R G+P+ F K R
Sbjct: 7 LLFVFLLFIAICFFLVHRHNSTTTKNLPPGSFGWPFIGETLAYIRSKRGGDPERFTKERI 66
Query: 104 AKYGN--VFKTSLIGNPTVVFGGAEGNRFLFSNENK 3
KYG+ VFKTS+ G VF G EGN+FLF NENK
Sbjct: 67 EKYGSTLVFKTSVAGERMAVFCGPEGNKFLFGNENK 102
>ref|XP_002266024.1| PREDICTED: cytochrome P450 716B2 [Vitis vinifera]
Length = 480
Score = 92.8 bits (229), Expect = 4e-17
Identities = 46/99 (46%), Positives = 69/99 (69%), Gaps = 5/99 (5%)
Frame = -2
Query: 284 MNMIYTAILAVLLCAFFL--KRKASLKGT--PPGSLGWPLLGETMEFLAQQRKGNPQEFV 117
++++ +L+V + FL K ++ G PPG +GWP++GE++EFL+ KG+P++F+
Sbjct: 6 LSLLLICVLSVSIGLQFLFYKHRSHFTGPNLPPGRIGWPMVGESLEFLSTGWKGHPEKFI 65
Query: 116 KSRAAKYGN-VFKTSLIGNPTVVFGGAEGNRFLFSNENK 3
R +KY + VFKTSL+G P VF GA GN+FLFSNENK
Sbjct: 66 FDRISKYSSEVFKTSLLGEPAAVFAGAAGNKFLFSNENK 104
>gb|AAX20147.1| taxane 13-alpha-hydroxylase [Taxus x media]
Length = 485
Score = 92.0 bits (227), Expect = 7e-17
Identities = 43/88 (48%), Positives = 64/88 (72%)
Frame = -2
Query: 266 AILAVLLCAFFLKRKASLKGTPPGSLGWPLLGETMEFLAQQRKGNPQEFVKSRAAKYGNV 87
A++A ++ F KR +S+K PPG+LG+PL+GET++F+ PQ+F++ R +K+G+V
Sbjct: 19 AVMAGIILFFRSKRHSSVK-LPPGNLGFPLVGETLQFVRSLGSSTPQQFIEERMSKFGDV 77
Query: 86 FKTSLIGNPTVVFGGAEGNRFLFSNENK 3
FKTS+IG+PTV G GNR + SNENK
Sbjct: 78 FKTSIIGHPTVALCGPAGNRLVLSNENK 105
>gb|AAX59903.1| 13-alpha-hydroxylase [Taxus wallichiana var. chinensis]
Length = 485
Score = 91.7 bits (226), Expect = 9e-17
Identities = 43/88 (48%), Positives = 64/88 (72%)
Frame = -2
Query: 266 AILAVLLCAFFLKRKASLKGTPPGSLGWPLLGETMEFLAQQRKGNPQEFVKSRAAKYGNV 87
A++ ++ F KR +S+K PPG+LG+PL+GET++F+ PQ+F++ R +K+G+V
Sbjct: 19 AVMVGIILFFRSKRHSSVK-LPPGNLGFPLVGETLQFVRTLGSSTPQQFIEERMSKFGDV 77
Query: 86 FKTSLIGNPTVVFGGAEGNRFLFSNENK 3
FKTS+IG+PTVV G GNR + SNENK
Sbjct: 78 FKTSIIGHPTVVLCGPAGNRLVLSNENK 105
>emb|CBI39273.3| unnamed protein product [Vitis vinifera]
Length = 933
Score = 91.7 bits (226), Expect = 9e-17
Identities = 44/95 (46%), Positives = 61/95 (64%), Gaps = 3/95 (3%)
Frame = -2
Query: 278 MIYTAILAVLLCAFFLKR--KASLKGTPPGSLGWPLLGETMEFLAQQRKGNPQEFVKSRA 105
+++ +L+V LC FL R K++ PPG+ GWP++GET+ F + GNP F+K R
Sbjct: 7 LLHPILLSVSLCVVFLVRRYKSATAKLPPGNQGWPIIGETLAFALGSKSGNPTRFIKERM 66
Query: 104 AKYG-NVFKTSLIGNPTVVFGGAEGNRFLFSNENK 3
KY +VF+TSL+G VF G GN+FLFSN NK
Sbjct: 67 MKYSPDVFRTSLVGEKVAVFCGPAGNKFLFSNHNK 101
Score = 82.8 bits (203), Expect = 4e-14
Identities = 38/77 (49%), Positives = 49/77 (63%), Gaps = 1/77 (1%)
Frame = -2
Query: 230 KRKASLKGTPPGSLGWPLLGETMEFLAQQRKGNPQEFVKSRAAKYGN-VFKTSLIGNPTV 54
+RK++ PPG LGWP++GET EF + NP+ F+ R KY VF+TSL+G
Sbjct: 482 RRKSACSKLPPGKLGWPIIGETSEFALGGKNSNPERFINDRMKKYSPIVFRTSLLGEKVA 541
Query: 53 VFGGAEGNRFLFSNENK 3
VF G GN+FLFSN NK
Sbjct: 542 VFCGPAGNKFLFSNHNK 558
>ref|XP_002279608.1| PREDICTED: cytochrome P450 716B2 [Vitis vinifera]
Length = 481
Score = 91.7 bits (226), Expect = 9e-17
Identities = 44/95 (46%), Positives = 61/95 (64%), Gaps = 3/95 (3%)
Frame = -2
Query: 278 MIYTAILAVLLCAFFLKR--KASLKGTPPGSLGWPLLGETMEFLAQQRKGNPQEFVKSRA 105
+++ +L+V LC FL R K++ PPG+ GWP++GET+ F + GNP F+K R
Sbjct: 7 LLHPILLSVSLCVVFLVRRYKSATAKLPPGNQGWPIIGETLAFALGSKSGNPTRFIKERM 66
Query: 104 AKYG-NVFKTSLIGNPTVVFGGAEGNRFLFSNENK 3
KY +VF+TSL+G VF G GN+FLFSN NK
Sbjct: 67 MKYSPDVFRTSLVGEKVAVFCGPAGNKFLFSNHNK 101