BLASTX nr result
ID: Ephedra28_contig00006186
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Ephedra28_contig00006186
(353 letters)
Database: ./nr
37,332,560 sequences; 13,225,080,153 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|Q50EK0.1|C16B2_PICSI RecName: Full=Cytochrome P450 716B2; Alt... 109 3e-22
sp|Q50EK1.1|C16B1_PICSI RecName: Full=Cytochrome P450 716B1; Alt... 109 3e-22
gb|ABR16707.1| unknown [Picea sitchensis] 100 2e-19
sp|Q8W4T9.1|T13H_TAXCU RecName: Full=Taxane 13-alpha-hydroxylase... 97 3e-18
gb|AAX20147.1| taxane 13-alpha-hydroxylase [Taxus x media] 95 9e-18
ref|XP_002965726.1| hypothetical protein SELMODRAFT_84634 [Selag... 95 9e-18
gb|AAX59903.1| 13-alpha-hydroxylase [Taxus wallichiana var. chin... 95 1e-17
emb|CBI39273.3| unnamed protein product [Vitis vinifera] 93 3e-17
ref|XP_002527982.1| cytochrome P450, putative [Ricinus communis]... 93 3e-17
ref|XP_002279608.1| PREDICTED: cytochrome P450 716B2 [Vitis vini... 93 3e-17
ref|NP_001268115.1| cytochrome P450 CYP716A15 [Vitis vinifera] g... 93 4e-17
gb|ABC94482.1| putative taxane 13-alpha-hydroxylase cytochrome P... 92 6e-17
ref|XP_002520384.1| cytochrome P450, putative [Ricinus communis]... 92 6e-17
ref|XP_002279623.1| PREDICTED: cytochrome P450 716B2 [Vitis vini... 92 6e-17
gb|AAS89065.2| taxoid 2-alpha-hydroxylase [Taxus canadensis] 92 7e-17
ref|XP_004239296.1| PREDICTED: cytochrome P450 716B2-like [Solan... 91 1e-16
ref|NP_001268076.1| cytochrome P450 CYP716A17 [Vitis vinifera] g... 91 1e-16
ref|XP_006340081.1| PREDICTED: cytochrome P450 716B1-like [Solan... 91 2e-16
ref|XP_006338129.1| PREDICTED: cytochrome P450 716B2-like [Solan... 91 2e-16
gb|ABK25038.1| unknown [Picea sitchensis] gi|324983873|gb|ADY687... 91 2e-16
>sp|Q50EK0.1|C16B2_PICSI RecName: Full=Cytochrome P450 716B2; AltName: Full=Cytochrome P450
CYPA2 gi|59800276|gb|AAX07437.1| cytochrome P450 CYPA2
[Picea sitchensis]
Length = 497
Score = 109 bits (273), Expect = 3e-22
Identities = 54/98 (55%), Positives = 67/98 (68%), Gaps = 6/98 (6%)
Frame = -1
Query: 278 MIYTAILAVLL-CAFFL-----KRKASMKGTPPGNLGWPLLGETMEFLAQRRNGNPQEFV 117
+++T LA + AFF R + +G PPG GWPL+GET+EFL +R GNP++F
Sbjct: 21 LMFTVFLASFIGLAFFFYLISNHRTKAWRGIPPGTFGWPLIGETLEFLGCQRKGNPRDFF 80
Query: 116 KSRAAKYGNVFKTSLIGKPTVVFGGAEGNRFIFSNENK 3
SR KYGNVF TSL+G PTVVF EGNRF+FSNENK
Sbjct: 81 DSRTQKYGNVFTTSLVGHPTVVFCSPEGNRFLFSNENK 118
>sp|Q50EK1.1|C16B1_PICSI RecName: Full=Cytochrome P450 716B1; AltName: Full=Cytochrome P450
CYPA1 gi|59800274|gb|AAX07436.1| cytochrome P450 CYPA1
[Picea sitchensis]
Length = 493
Score = 109 bits (273), Expect = 3e-22
Identities = 54/98 (55%), Positives = 67/98 (68%), Gaps = 6/98 (6%)
Frame = -1
Query: 278 MIYTAILAVLL-CAFFL-----KRKASMKGTPPGNLGWPLLGETMEFLAQRRNGNPQEFV 117
+++T LA + AFF R + +G PPG GWPL+GET+EFL +R GNP++F
Sbjct: 21 LMFTVFLASFIGLAFFFYLISNHRTKAWRGIPPGTFGWPLIGETLEFLGCQRKGNPRDFF 80
Query: 116 KSRAAKYGNVFKTSLIGKPTVVFGGAEGNRFIFSNENK 3
SR KYGNVF TSL+G PTVVF EGNRF+FSNENK
Sbjct: 81 DSRTQKYGNVFTTSLVGHPTVVFCSPEGNRFLFSNENK 118
>gb|ABR16707.1| unknown [Picea sitchensis]
Length = 490
Score = 100 bits (250), Expect = 2e-19
Identities = 46/87 (52%), Positives = 57/87 (65%)
Frame = -1
Query: 263 ILAVLLCAFFLKRKASMKGTPPGNLGWPLLGETMEFLAQRRNGNPQEFVKSRAAKYGNVF 84
I A R +G PPG GWPL+GET+EFL ++ G+P +F SR +YGNVF
Sbjct: 30 IFLFFFSAIIRHRNRVSRGIPPGTFGWPLIGETVEFLRCQKRGSPHQFFDSRTQRYGNVF 89
Query: 83 KTSLIGKPTVVFGGAEGNRFIFSNENK 3
T L+G PTVVF G EGNRF+F+NENK
Sbjct: 90 TTFLLGHPTVVFCGPEGNRFLFANENK 116
>sp|Q8W4T9.1|T13H_TAXCU RecName: Full=Taxane 13-alpha-hydroxylase; AltName: Full=Cytochrome
P450 725A2 gi|17148242|gb|AAL23619.1| taxane
13-alpha-hydroxylase [Taxus cuspidata]
Length = 485
Score = 96.7 bits (239), Expect = 3e-18
Identities = 47/106 (44%), Positives = 72/106 (67%)
Frame = -1
Query: 320 MGMWRTLQGGGNMNMIYTAILAVLLCAFFLKRKASMKGTPPGNLGWPLLGETMEFLAQRR 141
M + L+ ++ + A++A ++ F KR +S+K PPGNLG+PL+GET++F+
Sbjct: 1 MDALKQLEVSPSILFVTLAVMAGIILFFRSKRHSSVK-LPPGNLGFPLVGETLQFVRSLG 59
Query: 140 NGNPQEFVKSRAAKYGNVFKTSLIGKPTVVFGGAEGNRFIFSNENK 3
+ PQ+F++ R +K+G+VFKTS+IG PTVV G GNR + SNENK
Sbjct: 60 SSTPQQFIEERMSKFGDVFKTSIIGHPTVVLCGPAGNRLVLSNENK 105
>gb|AAX20147.1| taxane 13-alpha-hydroxylase [Taxus x media]
Length = 485
Score = 95.1 bits (235), Expect = 9e-18
Identities = 46/106 (43%), Positives = 71/106 (66%)
Frame = -1
Query: 320 MGMWRTLQGGGNMNMIYTAILAVLLCAFFLKRKASMKGTPPGNLGWPLLGETMEFLAQRR 141
M + L+ ++ + A++A ++ F KR +S+K PPGNLG+PL+GET++F+
Sbjct: 1 MDALKQLEVSPSILFVTLAVMAGIILFFRSKRHSSVK-LPPGNLGFPLVGETLQFVRSLG 59
Query: 140 NGNPQEFVKSRAAKYGNVFKTSLIGKPTVVFGGAEGNRFIFSNENK 3
+ PQ+F++ R +K+G+VFKTS+IG PTV G GNR + SNENK
Sbjct: 60 SSTPQQFIEERMSKFGDVFKTSIIGHPTVALCGPAGNRLVLSNENK 105
>ref|XP_002965726.1| hypothetical protein SELMODRAFT_84634 [Selaginella moellendorffii]
gi|300166540|gb|EFJ33146.1| hypothetical protein
SELMODRAFT_84634 [Selaginella moellendorffii]
Length = 469
Score = 95.1 bits (235), Expect = 9e-18
Identities = 44/94 (46%), Positives = 59/94 (62%)
Frame = -1
Query: 284 MNMIYTAILAVLLCAFFLKRKASMKGTPPGNLGWPLLGETMEFLAQRRNGNPQEFVKSRA 105
M ++ A +L+ F R + PPGNLGWP++GET++FLA R F R
Sbjct: 1 MILLLGACFLLLITTIFAIRFSPSSNLPPGNLGWPIVGETLQFLALFRKNKAYSFFHERM 60
Query: 104 AKYGNVFKTSLIGKPTVVFGGAEGNRFIFSNENK 3
AK+G VFKTSL+G PT+V G +GN+F+FS ENK
Sbjct: 61 AKHGGVFKTSLLGSPTIVMPGPDGNKFLFSQENK 94
>gb|AAX59903.1| 13-alpha-hydroxylase [Taxus wallichiana var. chinensis]
Length = 485
Score = 94.7 bits (234), Expect = 1e-17
Identities = 46/106 (43%), Positives = 71/106 (66%)
Frame = -1
Query: 320 MGMWRTLQGGGNMNMIYTAILAVLLCAFFLKRKASMKGTPPGNLGWPLLGETMEFLAQRR 141
M + L+ ++ + A++ ++ F KR +S+K PPGNLG+PL+GET++F+
Sbjct: 1 MDALKQLEVSPSILFVTLAVMVGIILFFRSKRHSSVK-LPPGNLGFPLVGETLQFVRTLG 59
Query: 140 NGNPQEFVKSRAAKYGNVFKTSLIGKPTVVFGGAEGNRFIFSNENK 3
+ PQ+F++ R +K+G+VFKTS+IG PTVV G GNR + SNENK
Sbjct: 60 SSTPQQFIEERMSKFGDVFKTSIIGHPTVVLCGPAGNRLVLSNENK 105
>emb|CBI39273.3| unnamed protein product [Vitis vinifera]
Length = 933
Score = 93.2 bits (230), Expect = 3e-17
Identities = 44/95 (46%), Positives = 63/95 (66%), Gaps = 3/95 (3%)
Frame = -1
Query: 278 MIYTAILAVLLCAFFLKR--KASMKGTPPGNLGWPLLGETMEFLAQRRNGNPQEFVKSRA 105
+++ +L+V LC FL R K++ PPGN GWP++GET+ F ++GNP F+K R
Sbjct: 7 LLHPILLSVSLCVVFLVRRYKSATAKLPPGNQGWPIIGETLAFALGSKSGNPTRFIKERM 66
Query: 104 AKYG-NVFKTSLIGKPTVVFGGAEGNRFIFSNENK 3
KY +VF+TSL+G+ VF G GN+F+FSN NK
Sbjct: 67 MKYSPDVFRTSLVGEKVAVFCGPAGNKFLFSNHNK 101
Score = 84.7 bits (208), Expect = 1e-14
Identities = 38/77 (49%), Positives = 51/77 (66%), Gaps = 1/77 (1%)
Frame = -1
Query: 230 KRKASMKGTPPGNLGWPLLGETMEFLAQRRNGNPQEFVKSRAAKYGN-VFKTSLIGKPTV 54
+RK++ PPG LGWP++GET EF +N NP+ F+ R KY VF+TSL+G+
Sbjct: 482 RRKSACSKLPPGKLGWPIIGETSEFALGGKNSNPERFINDRMKKYSPIVFRTSLLGEKVA 541
Query: 53 VFGGAEGNRFIFSNENK 3
VF G GN+F+FSN NK
Sbjct: 542 VFCGPAGNKFLFSNHNK 558
>ref|XP_002527982.1| cytochrome P450, putative [Ricinus communis]
gi|223532608|gb|EEF34394.1| cytochrome P450, putative
[Ricinus communis]
Length = 480
Score = 93.2 bits (230), Expect = 3e-17
Identities = 45/102 (44%), Positives = 69/102 (67%), Gaps = 9/102 (8%)
Frame = -1
Query: 281 NMIYTAILAVLLCAFFLKRKA--------SMKGTPPGNLGWPLLGETMEFLAQRRNGNPQ 126
++ +AI ++ L +FL K+ S+ TPPG GWPL+GET+E+L+ + G P+
Sbjct: 6 SVFISAIFSIALVLYFLNYKSKPRKDRAESLIPTPPGTTGWPLIGETLEYLSTTKAGIPE 65
Query: 125 EFVKSRAAKYGN-VFKTSLIGKPTVVFGGAEGNRFIFSNENK 3
+F+ R +Y + +FKTSL+G+PTV+ A+GN+FIFSNENK
Sbjct: 66 KFINDRRKRYSSKLFKTSLLGQPTVLLCNADGNKFIFSNENK 107
>ref|XP_002279608.1| PREDICTED: cytochrome P450 716B2 [Vitis vinifera]
Length = 481
Score = 93.2 bits (230), Expect = 3e-17
Identities = 44/95 (46%), Positives = 63/95 (66%), Gaps = 3/95 (3%)
Frame = -1
Query: 278 MIYTAILAVLLCAFFLKR--KASMKGTPPGNLGWPLLGETMEFLAQRRNGNPQEFVKSRA 105
+++ +L+V LC FL R K++ PPGN GWP++GET+ F ++GNP F+K R
Sbjct: 7 LLHPILLSVSLCVVFLVRRYKSATAKLPPGNQGWPIIGETLAFALGSKSGNPTRFIKERM 66
Query: 104 AKYG-NVFKTSLIGKPTVVFGGAEGNRFIFSNENK 3
KY +VF+TSL+G+ VF G GN+F+FSN NK
Sbjct: 67 MKYSPDVFRTSLVGEKVAVFCGPAGNKFLFSNHNK 101
>ref|NP_001268115.1| cytochrome P450 CYP716A15 [Vitis vinifera]
gi|326324797|dbj|BAJ84106.1| cytochrome P450 [Vitis
vinifera]
Length = 480
Score = 92.8 bits (229), Expect = 4e-17
Identities = 43/95 (45%), Positives = 66/95 (69%), Gaps = 3/95 (3%)
Frame = -1
Query: 278 MIYTAILAVLLCAFFLKRKASMKGT--PPGNLGWPLLGETMEFLAQRRNGNPQEFVKSRA 105
+I+ +++ L F K ++ G PPG +GWP++GE++EFL+ G+P++F+ R
Sbjct: 10 LIFVLSVSIGLHLLFYKHRSHFTGPNLPPGKIGWPMVGESLEFLSTGWKGHPEKFIFDRI 69
Query: 104 AKYGN-VFKTSLIGKPTVVFGGAEGNRFIFSNENK 3
+KY + VFKTSL+G+P VF GA GN+F+FSNENK
Sbjct: 70 SKYSSEVFKTSLLGEPAAVFAGAAGNKFLFSNENK 104
>gb|ABC94482.1| putative taxane 13-alpha-hydroxylase cytochrome P450 [Artemisia
annua]
Length = 480
Score = 92.4 bits (228), Expect = 6e-17
Identities = 42/96 (43%), Positives = 62/96 (64%), Gaps = 4/96 (4%)
Frame = -1
Query: 278 MIYTAILAVLLCAFFLKRKASM--KGTPPGNLGWPLLGETMEFLAQRRNGNPQEFVKSRA 105
+++ +L + +C F + R S K PPG+ GWP +GET+ ++ +R G+P+ F K R
Sbjct: 7 LLFVFLLFIAICFFLVHRHNSTTTKNLPPGSFGWPFIGETLAYIRSKRGGDPERFTKERI 66
Query: 104 AKYGN--VFKTSLIGKPTVVFGGAEGNRFIFSNENK 3
KYG+ VFKTS+ G+ VF G EGN+F+F NENK
Sbjct: 67 EKYGSTLVFKTSVAGERMAVFCGPEGNKFLFGNENK 102
>ref|XP_002520384.1| cytochrome P450, putative [Ricinus communis]
gi|223540431|gb|EEF42000.1| cytochrome P450, putative
[Ricinus communis]
Length = 379
Score = 92.4 bits (228), Expect = 6e-17
Identities = 50/96 (52%), Positives = 61/96 (63%), Gaps = 5/96 (5%)
Frame = -1
Query: 275 IYTAILAVLLCAFFLKRKAS----MKGTPPGNLGWPLLGETMEFL-AQRRNGNPQEFVKS 111
I+ +A+L+ FLK K + PPG LG P +GETMEF AQR N +EFV+
Sbjct: 14 IFCLAIAILISTMFLKHKLVHNKIKRRLPPGELGLPWIGETMEFFQAQRSNRLFEEFVQP 73
Query: 110 RAAKYGNVFKTSLIGKPTVVFGGAEGNRFIFSNENK 3
R AKYGN+FKT L+G PT+V GAE NRF SNE K
Sbjct: 74 RIAKYGNIFKTRLMGSPTIVVNGAEANRFFLSNEFK 109
>ref|XP_002279623.1| PREDICTED: cytochrome P450 716B2 [Vitis vinifera]
Length = 476
Score = 92.4 bits (228), Expect = 6e-17
Identities = 44/95 (46%), Positives = 61/95 (64%), Gaps = 3/95 (3%)
Frame = -1
Query: 278 MIYTAILAVLLCAFFL--KRKASMKGTPPGNLGWPLLGETMEFLAQRRNGNPQEFVKSRA 105
+++ +L V LC FL +RK++ PPG LGWP++GET EF +N NP+ F+ R
Sbjct: 7 LLHLIVLFVSLCIIFLVSRRKSACSKLPPGKLGWPIIGETSEFALGGKNSNPERFINDRM 66
Query: 104 AKYGN-VFKTSLIGKPTVVFGGAEGNRFIFSNENK 3
KY VF+TSL+G+ VF G GN+F+FSN NK
Sbjct: 67 KKYSPIVFRTSLLGEKVAVFCGPAGNKFLFSNHNK 101
>gb|AAS89065.2| taxoid 2-alpha-hydroxylase [Taxus canadensis]
Length = 495
Score = 92.0 bits (227), Expect = 7e-17
Identities = 46/100 (46%), Positives = 65/100 (65%)
Frame = -1
Query: 302 LQGGGNMNMIYTAILAVLLCAFFLKRKASMKGTPPGNLGWPLLGETMEFLAQRRNGNPQE 123
LQ + + + I+ +++ KR++S+K PPG LG PL+GE++ FL R+ ++
Sbjct: 17 LQSSAILLTVVSGIIVIVILLLRSKRRSSLK-LPPGKLGLPLIGESLSFLWALRSNTLEQ 75
Query: 122 FVKSRAAKYGNVFKTSLIGKPTVVFGGAEGNRFIFSNENK 3
FV R KYGNVFKTSL+G+PTVV GA GNR I SN+ K
Sbjct: 76 FVDKRVKKYGNVFKTSLLGQPTVVLCGAAGNRLILSNQEK 115
>ref|XP_004239296.1| PREDICTED: cytochrome P450 716B2-like [Solanum lycopersicum]
Length = 476
Score = 91.3 bits (225), Expect = 1e-16
Identities = 44/98 (44%), Positives = 64/98 (65%), Gaps = 4/98 (4%)
Frame = -1
Query: 284 MNMIYTAILAV---LLCAFFLKRKASMKGTPPGNLGWPLLGETMEFLAQRRNGNPQEFVK 114
M ++Y ++ V L+ L +K S +G PPG GWP+ GE++EFL+ G+P++F+
Sbjct: 1 MELLYVCLVCVFVFLVSLLLLYKKKSGEGLPPGKTGWPVFGESLEFLSSGWKGHPEKFIF 60
Query: 113 SRAAKY-GNVFKTSLIGKPTVVFGGAEGNRFIFSNENK 3
R AKY +VFKT L+G+ VF GA N+F+FSNENK
Sbjct: 61 DRVAKYSSSVFKTHLLGEEAAVFCGASANKFLFSNENK 98
>ref|NP_001268076.1| cytochrome P450 CYP716A17 [Vitis vinifera]
gi|326324799|dbj|BAJ84107.1| Cytochrome P450 [Vitis
vinifera]
Length = 480
Score = 91.3 bits (225), Expect = 1e-16
Identities = 43/99 (43%), Positives = 70/99 (70%), Gaps = 5/99 (5%)
Frame = -1
Query: 284 MNMIYTAILAVLLCAFFL--KRKASMKGT--PPGNLGWPLLGETMEFLAQRRNGNPQEFV 117
++++ ++L+V + + L K ++ G PPG +GWP++GE++EFL+ G+P++F+
Sbjct: 6 LSLLLISVLSVSIRLYLLLYKHRSHFTGPNLPPGKIGWPMVGESLEFLSTGWKGHPEKFI 65
Query: 116 KSRAAKYGN-VFKTSLIGKPTVVFGGAEGNRFIFSNENK 3
R +KY + VFKTSL+G+P VF GA GN+F+FSNENK
Sbjct: 66 FDRISKYSSEVFKTSLLGEPAAVFAGAAGNKFLFSNENK 104
>ref|XP_006340081.1| PREDICTED: cytochrome P450 716B1-like [Solanum tuberosum]
Length = 474
Score = 90.9 bits (224), Expect = 2e-16
Identities = 43/87 (49%), Positives = 59/87 (67%), Gaps = 1/87 (1%)
Frame = -1
Query: 260 LAVLLCAFFLKRKASMKGTPPGNLGWPLLGETMEFLAQRRNGNPQEFVKSRAAKY-GNVF 84
LA+ + + R+ + PPG+ GWP LGET++FL + G P++FVK R KY +F
Sbjct: 12 LALFIIIVLVMRRKRLN-YPPGSCGWPFLGETLKFLKANKEGKPEKFVKERIEKYKSKIF 70
Query: 83 KTSLIGKPTVVFGGAEGNRFIFSNENK 3
KTSL+G+ VV GGA GN+F+FSNENK
Sbjct: 71 KTSLMGETVVVLGGASGNKFLFSNENK 97
>ref|XP_006338129.1| PREDICTED: cytochrome P450 716B2-like [Solanum tuberosum]
Length = 476
Score = 90.9 bits (224), Expect = 2e-16
Identities = 44/98 (44%), Positives = 64/98 (65%), Gaps = 4/98 (4%)
Frame = -1
Query: 284 MNMIYTAILAV---LLCAFFLKRKASMKGTPPGNLGWPLLGETMEFLAQRRNGNPQEFVK 114
M +Y +++ V L+ L + S +G PPG GWP+ GE++EFL+ G+P++F+
Sbjct: 1 MEFLYVSLVCVFVFLVSLLLLYKNKSGEGLPPGKTGWPVFGESLEFLSTGWKGHPEKFIF 60
Query: 113 SRAAKY-GNVFKTSLIGKPTVVFGGAEGNRFIFSNENK 3
R AKY +VFKT L+G+ VF GA GN+F+FSNENK
Sbjct: 61 DRVAKYSSSVFKTHLLGEEAAVFCGASGNKFLFSNENK 98
>gb|ABK25038.1| unknown [Picea sitchensis] gi|324983873|gb|ADY68773.1| cytochrome
P450 90A20 [Picea sitchensis]
Length = 487
Score = 90.9 bits (224), Expect = 2e-16
Identities = 42/88 (47%), Positives = 57/88 (64%)
Frame = -1
Query: 266 AILAVLLCAFFLKRKASMKGTPPGNLGWPLLGETMEFLAQRRNGNPQEFVKSRAAKYGNV 87
A L + L F K + + KG PPG++GWPLLGET++ +A + NP+ FV +R ++YG V
Sbjct: 20 AFLVLFLQWVFGKGRRTRKGLPPGSMGWPLLGETLQLIAAYKTSNPEPFVDTRRSRYGKV 79
Query: 86 FKTSLIGKPTVVFGGAEGNRFIFSNENK 3
FKT L GKPT+ E N+FI NE K
Sbjct: 80 FKTHLFGKPTIFSTDPEVNKFILQNEGK 107