BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Ephedra28_contig00005521
         (677 letters)

Database: ./nr 
           37,332,560 sequences; 13,225,080,153 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|NP_196114.1| nicotianamine synthase 1 [Arabidopsis thaliana]...   136   5e-30
dbj|BAA74589.1| nicotianamine synthase [Arabidopsis thaliana]         136   5e-30
ref|XP_006289941.1| hypothetical protein CARUB_v10003563mg [Caps...   136   7e-30
gb|AFH08365.1| nicotianamine synthase 1, partial [Arabidopsis ha...   135   9e-30
ref|XP_002873177.1| hypothetical protein ARALYDRAFT_487282 [Arab...   135   9e-30
ref|XP_006398952.1| hypothetical protein EUTSA_v10014123mg [Eutr...   135   1e-29
ref|XP_003558078.1| PREDICTED: nicotianamine synthase 8-like iso...   135   1e-29
gb|AFK39325.1| unknown [Lotus japonicus]                              134   3e-29
dbj|BAH22562.1| nicotianamine synthase [Lotus japonicus]              134   3e-29
ref|XP_006401382.1| hypothetical protein EUTSA_v10013812mg [Eutr...   133   4e-29
tpg|DAA45019.1| TPA: hypothetical protein ZEAMMB73_285183 [Zea m...   133   4e-29
gb|ESW35316.1| hypothetical protein PHAVU_001G225000g [Phaseolus...   133   6e-29
gb|AEF97346.1| nicotianamine synthase 4 [Noccaea caerulescens]        133   6e-29
ref|XP_006840459.1| hypothetical protein AMTR_s00045p00177240 [A...   132   1e-28
ref|XP_004984619.1| PREDICTED: nicotianamine synthase 2-like [Se...   132   1e-28
gb|AFH08366.1| nicotianamine synthase 2 [Arabidopsis halleri sub...   132   1e-28
ref|XP_006392427.1| hypothetical protein EUTSA_v10024166mg [Eutr...   131   2e-28
sp|Q9XFB6.1|NAS8_HORVU RecName: Full=Nicotianamine synthase 8; A...   131   2e-28
gb|EXB98412.1| Nicotianamine synthase [Morus notabilis]               130   4e-28
ref|XP_006382395.1| hypothetical protein POPTR_0005s01730g [Popu...   130   4e-28

>ref|NP_196114.1| nicotianamine synthase 1 [Arabidopsis thaliana]
           gi|27151631|sp|Q9FF79.1|NAS1_ARATH RecName:
           Full=Nicotianamine synthase 1; AltName:
           Full=S-adenosyl-L-methionine:S-adenosyl-L-methionine:S-
           adenosyl-methionine 3-amino-3-carboxypropyltransferase
           1; Short=AtNAS1 gi|10178034|dbj|BAB11517.1|
           nicotianamine synthase [Arabidopsis thaliana]
           gi|18252859|gb|AAL62356.1| nicotianamine synthase
           [Arabidopsis thaliana] gi|23198368|gb|AAN15711.1|
           nicotianamine synthase [Arabidopsis thaliana]
           gi|332003425|gb|AED90808.1| nicotianamine synthase 1
           [Arabidopsis thaliana]
          Length = 320

 Score =  136 bits (343), Expect = 5e-30
 Identities = 80/185 (43%), Positives = 116/185 (62%), Gaps = 1/185 (0%)
 Frame = +1

Query: 112 VVERVRGLYGKICKLQGSLKPSQNVNALFGELVSVCGGAQGRNVRVDKLPSADRVWRFHL 291
           VV+++  LY +I KL+ SLKPS+NV+ LFG+LVS C      N+ V  +    +  R +L
Sbjct: 8   VVKQIIDLYDQISKLK-SLKPSKNVDTLFGQLVSTCLPTD-TNIDVTNMCEEVKDMRANL 65

Query: 292 IQLCSESEGELERHYSRLLGKLERPCSP-ESLRMFPYYDNYVKLSKLEYTTLCMAAPEMS 468
           I+LC E+EG LE+H+S +LG L+   +P + L +FPYY NY+KL KLE+  L        
Sbjct: 66  IKLCGEAEGYLEQHFSTILGSLQEDQNPLDHLHIFPYYSNYLKLGKLEFDLL-----SQH 120

Query: 469 ASKESKRVAFFGSGPLPLSTIVMAMGNYFGEHAVFDNYDMDSSANDLARNLVEPHEMLAN 648
           +S    ++AF GSGP+PL++IV+A   +   +  F N+D+DS AN LA NLV     L+ 
Sbjct: 121 SSHVPTKIAFVGSGPMPLTSIVLA--KFHLPNTTFHNFDIDSHANTLASNLVSRDPDLSK 178

Query: 649 KMFFH 663
           +M FH
Sbjct: 179 RMIFH 183


>dbj|BAA74589.1| nicotianamine synthase [Arabidopsis thaliana]
          Length = 320

 Score =  136 bits (343), Expect = 5e-30
 Identities = 80/185 (43%), Positives = 116/185 (62%), Gaps = 1/185 (0%)
 Frame = +1

Query: 112 VVERVRGLYGKICKLQGSLKPSQNVNALFGELVSVCGGAQGRNVRVDKLPSADRVWRFHL 291
           VV+++  LY +I KL+ SLKPS+NV+ LFG+LVS C      N+ V  +    +  R +L
Sbjct: 8   VVKQIIDLYDQISKLK-SLKPSKNVDTLFGQLVSTCLPTD-TNIDVTNMCEEVKDMRANL 65

Query: 292 IQLCSESEGELERHYSRLLGKLERPCSP-ESLRMFPYYDNYVKLSKLEYTTLCMAAPEMS 468
           I+LC E+EG LE+H+S +LG L+   +P + L +FPYY NY+KL KLE+  L        
Sbjct: 66  IKLCGEAEGYLEQHFSTILGSLQEDQNPLDHLHIFPYYSNYLKLGKLEFDLL-----SQH 120

Query: 469 ASKESKRVAFFGSGPLPLSTIVMAMGNYFGEHAVFDNYDMDSSANDLARNLVEPHEMLAN 648
           +S    ++AF GSGP+PL++IV+A   +   +  F N+D+DS AN LA NLV     L+ 
Sbjct: 121 SSHVPTKIAFVGSGPMPLTSIVLA--KFHLPNTTFHNFDIDSHANTLASNLVSRDPDLSK 178

Query: 649 KMFFH 663
           +M FH
Sbjct: 179 RMIFH 183


>ref|XP_006289941.1| hypothetical protein CARUB_v10003563mg [Capsella rubella]
           gi|482558647|gb|EOA22839.1| hypothetical protein
           CARUB_v10003563mg [Capsella rubella]
          Length = 321

 Score =  136 bits (342), Expect = 7e-30
 Identities = 80/185 (43%), Positives = 116/185 (62%), Gaps = 1/185 (0%)
 Frame = +1

Query: 112 VVERVRGLYGKICKLQGSLKPSQNVNALFGELVSVCGGAQGRNVRVDKLPSADRVWRFHL 291
           VV+++  LY +I KL+ SL+PS+NV+ LFGELVS C      N+ V KL    +  R +L
Sbjct: 8   VVKQIIDLYDQISKLE-SLRPSKNVDTLFGELVSTCLPTD-TNIDVTKLSEEVKDMRSNL 65

Query: 292 IQLCSESEGELERHYSRLLGKLERPCSP-ESLRMFPYYDNYVKLSKLEYTTLCMAAPEMS 468
           I+LC E+EG LE+H+S +LG L    +P + L +FPY++NY+KL KLE+  L        
Sbjct: 66  IKLCGEAEGYLEQHFSTILGSLPEDQNPLDHLHIFPYFNNYLKLGKLEFDLL-----SQH 120

Query: 469 ASKESKRVAFFGSGPLPLSTIVMAMGNYFGEHAVFDNYDMDSSANDLARNLVEPHEMLAN 648
           +S    ++AF GSGP+PL++IV+A   +   +  F N+D+DS AN LA  LV     L+ 
Sbjct: 121 SSHVPSKIAFVGSGPMPLTSIVLA--KFHLPNTTFHNFDIDSHANTLASRLVSRDPDLSK 178

Query: 649 KMFFH 663
           +M FH
Sbjct: 179 RMIFH 183


>gb|AFH08365.1| nicotianamine synthase 1, partial [Arabidopsis halleri subsp.
           halleri]
          Length = 322

 Score =  135 bits (341), Expect = 9e-30
 Identities = 80/185 (43%), Positives = 115/185 (62%), Gaps = 1/185 (0%)
 Frame = +1

Query: 112 VVERVRGLYGKICKLQGSLKPSQNVNALFGELVSVCGGAQGRNVRVDKLPSADRVWRFHL 291
           VV+++  LY +I KL  SLKPS+NV+ LFG+LVS C      N+ V  +    +  R +L
Sbjct: 8   VVKQIVDLYDQISKLV-SLKPSKNVDTLFGQLVSTCLPTD-TNIDVTNMCEEVKYMRSNL 65

Query: 292 IQLCSESEGELERHYSRLLGKLERPCSP-ESLRMFPYYDNYVKLSKLEYTTLCMAAPEMS 468
           I+LC E+EG LE+H+S +LG L+   +P + L +FPYY NY+KL KLE+  L        
Sbjct: 66  IKLCGEAEGYLEQHFSTILGSLQEDQNPLDHLHIFPYYTNYLKLGKLEFDLL-----SQH 120

Query: 469 ASKESKRVAFFGSGPLPLSTIVMAMGNYFGEHAVFDNYDMDSSANDLARNLVEPHEMLAN 648
           +S    ++AF GSGP+PL++IV+A   +   +  F N+D+DS AN LA NLV     L+ 
Sbjct: 121 SSHVPTKIAFVGSGPMPLTSIVLA--KFHLPNTTFHNFDIDSHANTLASNLVSRDPDLSK 178

Query: 649 KMFFH 663
           +M FH
Sbjct: 179 RMIFH 183


>ref|XP_002873177.1| hypothetical protein ARALYDRAFT_487282 [Arabidopsis lyrata subsp.
           lyrata] gi|297319014|gb|EFH49436.1| hypothetical protein
           ARALYDRAFT_487282 [Arabidopsis lyrata subsp. lyrata]
          Length = 320

 Score =  135 bits (341), Expect = 9e-30
 Identities = 80/185 (43%), Positives = 115/185 (62%), Gaps = 1/185 (0%)
 Frame = +1

Query: 112 VVERVRGLYGKICKLQGSLKPSQNVNALFGELVSVCGGAQGRNVRVDKLPSADRVWRFHL 291
           VV+++  LY +I KL  SLKPS+NV+ LFG+LVS C      N+ V  +    +  R +L
Sbjct: 8   VVKQIVDLYDQISKLV-SLKPSKNVDTLFGQLVSTCLPTD-TNIDVTNMCEEVKYMRSNL 65

Query: 292 IQLCSESEGELERHYSRLLGKLERPCSP-ESLRMFPYYDNYVKLSKLEYTTLCMAAPEMS 468
           I+LC E+EG LE+H+S +LG L+   +P + L +FPYY NY+KL KLE+  L        
Sbjct: 66  IKLCGEAEGYLEQHFSTILGSLQEDQNPLDHLHIFPYYTNYLKLGKLEFDLL-----SQH 120

Query: 469 ASKESKRVAFFGSGPLPLSTIVMAMGNYFGEHAVFDNYDMDSSANDLARNLVEPHEMLAN 648
           +S    ++AF GSGP+PL++IV+A   +   +  F N+D+DS AN LA NLV     L+ 
Sbjct: 121 SSHVPTKIAFVGSGPMPLTSIVLA--KFHLPNTTFHNFDIDSHANTLASNLVSRDPDLSK 178

Query: 649 KMFFH 663
           +M FH
Sbjct: 179 RMIFH 183


>ref|XP_006398952.1| hypothetical protein EUTSA_v10014123mg [Eutrema salsugineum]
           gi|557100042|gb|ESQ40405.1| hypothetical protein
           EUTSA_v10014123mg [Eutrema salsugineum]
          Length = 321

 Score =  135 bits (340), Expect = 1e-29
 Identities = 79/185 (42%), Positives = 116/185 (62%), Gaps = 1/185 (0%)
 Frame = +1

Query: 112 VVERVRGLYGKICKLQGSLKPSQNVNALFGELVSVCGGAQGRNVRVDKLPSADRVWRFHL 291
           VV+++  LY +I KL+ SLKPS+NV+ LFG+LVS C      N+ V  +    +  R  L
Sbjct: 8   VVKQIIDLYDQISKLE-SLKPSKNVDTLFGQLVSTCLPTD-TNIDVTNMSEEVKDMRSSL 65

Query: 292 IQLCSESEGELERHYSRLLGKLERPCSP-ESLRMFPYYDNYVKLSKLEYTTLCMAAPEMS 468
           I+LC E+EG LE+H+S +LG L+   +P + L +FPYY+NY+KL KLE+  L        
Sbjct: 66  IKLCGEAEGYLEQHFSTILGSLQEDQNPLDHLHIFPYYNNYLKLGKLEFDLL-----SQH 120

Query: 469 ASKESKRVAFFGSGPLPLSTIVMAMGNYFGEHAVFDNYDMDSSANDLARNLVEPHEMLAN 648
           +S    ++AF GSGP+PL++IV+A   +   +  F N+D+DS AN LA +LV     L+ 
Sbjct: 121 SSHVPTKIAFVGSGPMPLTSIVLA--KFHLPNTTFHNFDIDSHANTLASSLVSRDPDLSK 178

Query: 649 KMFFH 663
           +M FH
Sbjct: 179 RMIFH 183


>ref|XP_003558078.1| PREDICTED: nicotianamine synthase 8-like isoform 1 [Brachypodium
           distachyon] gi|357112565|ref|XP_003558079.1| PREDICTED:
           nicotianamine synthase 8-like isoform 2 [Brachypodium
           distachyon]
          Length = 333

 Score =  135 bits (339), Expect = 1e-29
 Identities = 82/184 (44%), Positives = 109/184 (59%)
 Frame = +1

Query: 112 VVERVRGLYGKICKLQGSLKPSQNVNALFGELVSVCGGAQGRNVRVDKLPSADRVWRFHL 291
           +V+++  L+  I KL  SL PS  V+ALF ELV+ C  A    V V KL    +  R  L
Sbjct: 11  LVQKITVLHAAIAKLP-SLSPSPEVDALFTELVTACVPASP--VDVTKLSPEAQKMREGL 67

Query: 292 IQLCSESEGELERHYSRLLGKLERPCSPESLRMFPYYDNYVKLSKLEYTTLCMAAPEMSA 471
           I+LCSE+EG+LE HYS +L   + P   + L MFPYY NY+ LSKLEY  L    P   +
Sbjct: 68  IRLCSEAEGKLEAHYSDMLAAFDNPL--DHLGMFPYYSNYINLSKLEYELLARYVPSSVS 125

Query: 472 SKESKRVAFFGSGPLPLSTIVMAMGNYFGEHAVFDNYDMDSSANDLARNLVEPHEMLANK 651
               +RVAF GSGPLP S++V+A  +  G   +FDNYD+  +AND A  LV   + +  +
Sbjct: 126 PAPPRRVAFIGSGPLPFSSLVLAARHLPG--TMFDNYDLCGAANDRASKLVRGDKDVGAR 183

Query: 652 MFFH 663
           M FH
Sbjct: 184 MSFH 187


>gb|AFK39325.1| unknown [Lotus japonicus]
          Length = 318

 Score =  134 bits (336), Expect = 3e-29
 Identities = 81/184 (44%), Positives = 111/184 (60%)
 Frame = +1

Query: 112 VVERVRGLYGKICKLQGSLKPSQNVNALFGELVSVCGGAQGRNVRVDKLPSADRVWRFHL 291
           ++E+V  LY +I  L+ SLKPS+NV+ LF ELV  C       + V  L    +  R HL
Sbjct: 8   LIEKVCSLYSQISTLE-SLKPSKNVDTLFTELVLTC--MPPSPIDVTNLTKNVQDIRSHL 64

Query: 292 IQLCSESEGELERHYSRLLGKLERPCSPESLRMFPYYDNYVKLSKLEYTTLCMAAPEMSA 471
           I+LC E+EG LE HYS +LG  + P   + L +FPYY+NY+KL  LEYT L       ++
Sbjct: 65  IRLCGEAEGHLESHYSTILGSHKNPL--DHLHIFPYYNNYLKLGLLEYTIL-----TQNS 117

Query: 472 SKESKRVAFFGSGPLPLSTIVMAMGNYFGEHAVFDNYDMDSSANDLARNLVEPHEMLANK 651
               +++AF GSGPLPL++IV+A  +       F NYD+DSSAN  A+ LV     L+N+
Sbjct: 118 IHVPEKIAFIGSGPLPLTSIVLASNHLIS--TTFHNYDIDSSANSSAQKLVLSDPDLSNR 175

Query: 652 MFFH 663
           M FH
Sbjct: 176 MVFH 179


>dbj|BAH22562.1| nicotianamine synthase [Lotus japonicus]
          Length = 318

 Score =  134 bits (336), Expect = 3e-29
 Identities = 81/184 (44%), Positives = 111/184 (60%)
 Frame = +1

Query: 112 VVERVRGLYGKICKLQGSLKPSQNVNALFGELVSVCGGAQGRNVRVDKLPSADRVWRFHL 291
           ++E+V  LY +I  L+ SLKPS+NV+ LF ELV  C       + V  L    +  R HL
Sbjct: 8   LIEKVCSLYSQISTLE-SLKPSKNVDTLFTELVLTC--MPPSPIDVTNLTKNVQDIRSHL 64

Query: 292 IQLCSESEGELERHYSRLLGKLERPCSPESLRMFPYYDNYVKLSKLEYTTLCMAAPEMSA 471
           I+LC E+EG LE HYS +LG  + P   + L +FPYY+NY+KL  LEYT L       ++
Sbjct: 65  IRLCGEAEGHLESHYSTILGSHKNPL--DHLHIFPYYNNYLKLGLLEYTIL-----TQNS 117

Query: 472 SKESKRVAFFGSGPLPLSTIVMAMGNYFGEHAVFDNYDMDSSANDLARNLVEPHEMLANK 651
               +++AF GSGPLPL++IV+A  +       F NYD+DSSAN  A+ LV     L+N+
Sbjct: 118 IHVPEKIAFIGSGPLPLTSIVLASNHLIS--TTFHNYDIDSSANSSAQKLVLSDPDLSNR 175

Query: 652 MFFH 663
           M FH
Sbjct: 176 MVFH 179


>ref|XP_006401382.1| hypothetical protein EUTSA_v10013812mg [Eutrema salsugineum]
           gi|557102472|gb|ESQ42835.1| hypothetical protein
           EUTSA_v10013812mg [Eutrema salsugineum]
          Length = 382

 Score =  133 bits (335), Expect = 4e-29
 Identities = 81/201 (40%), Positives = 123/201 (61%), Gaps = 5/201 (2%)
 Frame = +1

Query: 76  SIGVENLDLGTE---VVERVRGLYGKICKLQGSLKPSQNVNALFGELVSVCGGAQGRNVR 246
           S+ + ++D+  +   VV+++  LY +I  L+ SLKPS+NV+ LFG+LVS C      N+ 
Sbjct: 55  SLSLHSVDMACQDNLVVKQIMDLYNQISNLE-SLKPSKNVDTLFGQLVSTCLPTD-TNID 112

Query: 247 VDKLPSAD-RVWRFHLIQLCSESEGELERHYSRLLGKLE-RPCSPESLRMFPYYDNYVKL 420
           V K+     +  R HLI+LC E+EG LE+H+S+++G  E  P +   L +FPYY+NY+KL
Sbjct: 113 VKKIHDEKVKDMRSHLIKLCGEAEGYLEQHFSKIIGSFEDNPLN--HLHIFPYYNNYLKL 170

Query: 421 SKLEYTTLCMAAPEMSASKESKRVAFFGSGPLPLSTIVMAMGNYFGEHAVFDNYDMDSSA 600
           SKLE+  L         S    ++AF GSGP+PL++IV+A   +   +    N+D+DS A
Sbjct: 171 SKLEFDLLA-----QHTSHVPSKIAFIGSGPMPLTSIVLA--KFHLPNTTIHNFDIDSQA 223

Query: 601 NDLARNLVEPHEMLANKMFFH 663
           N LA N+V     L+ +M FH
Sbjct: 224 NTLASNIVSRDPDLSKRMVFH 244


>tpg|DAA45019.1| TPA: hypothetical protein ZEAMMB73_285183 [Zea mays]
          Length = 601

 Score =  133 bits (335), Expect = 4e-29
 Identities = 82/184 (44%), Positives = 112/184 (60%)
 Frame = +1

Query: 112 VVERVRGLYGKICKLQGSLKPSQNVNALFGELVSVCGGAQGRNVRVDKLPSADRVWRFHL 291
           +V+++ GL+  I KL  SL PS +V+ALF ELV  C       V V KL +  +  R  L
Sbjct: 289 LVQKITGLHAAINKLP-SLSPSPDVDALFTELVMAC--VPPSPVDVTKLGTDAQRMREEL 345

Query: 292 IQLCSESEGELERHYSRLLGKLERPCSPESLRMFPYYDNYVKLSKLEYTTLCMAAPEMSA 471
           I+LCS++EG LE HY+ +L   + P   + L  FPY++NYV LSKLEY  L    P ++ 
Sbjct: 346 IRLCSDAEGHLEAHYADMLAAFDNPL--DHLGRFPYFNNYVNLSKLEYDLLVRYVPGIAP 403

Query: 472 SKESKRVAFFGSGPLPLSTIVMAMGNYFGEHAVFDNYDMDSSANDLARNLVEPHEMLANK 651
           S    R+AF GSGPLP S++V+A  +    + +FDNYD  ++AND AR LV   E L  +
Sbjct: 404 S----RIAFVGSGPLPFSSLVLASRHL--PNVMFDNYDRCAAANDRARKLVRADEGLRKR 457

Query: 652 MFFH 663
           MFFH
Sbjct: 458 MFFH 461



 Score =  125 bits (315), Expect = 9e-27
 Identities = 79/184 (42%), Positives = 106/184 (57%)
 Frame = +1

Query: 112 VVERVRGLYGKICKLQGSLKPSQNVNALFGELVSVCGGAQGRNVRVDKLPSADRVWRFHL 291
           +V+++  L+  I KL  SL PS +VNALF  LV  C       V V KL    +  R  L
Sbjct: 11  LVKKIADLHADITKLP-SLSPSPDVNALFTSLVMAC--VPPSTVDVTKLSPDSQRMREEL 67

Query: 292 IQLCSESEGELERHYSRLLGKLERPCSPESLRMFPYYDNYVKLSKLEYTTLCMAAPEMSA 471
           I+LCS++EG LE HY+ +L   + P   + L  FPY+ NY+ LSKLEY  L    P ++ 
Sbjct: 68  IRLCSDAEGHLEAHYADMLAAFDNPL--DHLGRFPYFSNYINLSKLEYDLLVRYIPGLAP 125

Query: 472 SKESKRVAFFGSGPLPLSTIVMAMGNYFGEHAVFDNYDMDSSANDLARNLVEPHEMLANK 651
           S    RVAF GSGPLP S++V+A  +    +  FDNYD  ++AND AR LV   + L  +
Sbjct: 126 S----RVAFVGSGPLPFSSLVLAARHL--PNTTFDNYDRCAAANDRARKLVRADKDLNAR 179

Query: 652 MFFH 663
           M FH
Sbjct: 180 MSFH 183


>gb|ESW35316.1| hypothetical protein PHAVU_001G225000g [Phaseolus vulgaris]
          Length = 317

 Score =  133 bits (334), Expect = 6e-29
 Identities = 81/184 (44%), Positives = 107/184 (58%)
 Frame = +1

Query: 112 VVERVRGLYGKICKLQGSLKPSQNVNALFGELVSVCGGAQGRNVRVDKLPSADRVWRFHL 291
           ++E+V GLY +I  L+ SLKP +NV+ LF +LV  C       + V  L    +  R HL
Sbjct: 8   LIEKVCGLYEQISSLE-SLKPCKNVDMLFTQLVLTC--IPPSPIDVTNLNKNVQEIRSHL 64

Query: 292 IQLCSESEGELERHYSRLLGKLERPCSPESLRMFPYYDNYVKLSKLEYTTLCMAAPEMSA 471
           I+LC E+EG LE HYS LL   + P   E+L +FPYY NY+KL  LE+T L      +  
Sbjct: 65  IKLCGEAEGHLETHYSTLLASYDNPI--ENLDIFPYYSNYLKLGLLEFTILTQHCTHVPT 122

Query: 472 SKESKRVAFFGSGPLPLSTIVMAMGNYFGEHAVFDNYDMDSSANDLARNLVEPHEMLANK 651
                ++AF GSGPLPL++IV+A  +       F NYDMDS AN  A  LV  H  L+N+
Sbjct: 123 -----KIAFVGSGPLPLTSIVLASNHL--PSTTFHNYDMDSLANSNATRLVSSHPDLSNR 175

Query: 652 MFFH 663
           M FH
Sbjct: 176 MLFH 179


>gb|AEF97346.1| nicotianamine synthase 4 [Noccaea caerulescens]
          Length = 322

 Score =  133 bits (334), Expect = 6e-29
 Identities = 80/186 (43%), Positives = 116/186 (62%), Gaps = 1/186 (0%)
 Frame = +1

Query: 109 EVVERVRGLYGKICKLQGSLKPSQNVNALFGELVSVCGGAQGRNVRVDKLPSADRVWRFH 288
           ++V  +  LY KI KL+ +LKP Q+V+ LF +LVS C      N+ V K+  + +  R +
Sbjct: 8   QLVSTICDLYEKISKLE-TLKPCQDVDTLFKQLVSTCI-PPNPNIDVTKMSESIQEMRSN 65

Query: 289 LIQLCSESEGELERHYSRLLGKLERPCSP-ESLRMFPYYDNYVKLSKLEYTTLCMAAPEM 465
           LI+LC E+EG LE H++ +L   E   +P   L +FPYYDNY+KLSKLE+  L     + 
Sbjct: 66  LIKLCGEAEGHLEHHFTSILNSFED--NPLHHLNLFPYYDNYLKLSKLEFDLL----EQN 119

Query: 466 SASKESKRVAFFGSGPLPLSTIVMAMGNYFGEHAVFDNYDMDSSANDLARNLVEPHEMLA 645
                 K VAF GSGPLPL++IV+A  +Y  + ++F N+D+DS+AN +A  LV     L+
Sbjct: 120 LKGSVPKTVAFIGSGPLPLTSIVLA--SYHLKDSIFHNFDIDSTANSVAARLVSSDPDLS 177

Query: 646 NKMFFH 663
            +MFFH
Sbjct: 178 QRMFFH 183


>ref|XP_006840459.1| hypothetical protein AMTR_s00045p00177240 [Amborella trichopoda]
           gi|548842177|gb|ERN02134.1| hypothetical protein
           AMTR_s00045p00177240 [Amborella trichopoda]
          Length = 327

 Score =  132 bits (332), Expect = 1e-28
 Identities = 80/185 (43%), Positives = 110/185 (59%)
 Frame = +1

Query: 112 VVERVRGLYGKICKLQGSLKPSQNVNALFGELVSVCGGAQGRNVRVDKLPSADRVWRFHL 291
           +V +V  LY KI KL+ SL PS  VN LF +LVS+C      ++ V KL    +  R  L
Sbjct: 8   LVTKVCELYDKISKLE-SLSPSPEVNTLFTQLVSIC--TPPSSIDVSKLSPKVQEIRAKL 64

Query: 292 IQLCSESEGELERHYSRLLGKLERPCSPESLRMFPYYDNYVKLSKLEYTTLCMAAPEMSA 471
           I+LC E+EG LE H+S +L   E P   + L +FPY  NY+KLS+LE+  L         
Sbjct: 65  IRLCGEAEGLLEAHFSSILASFESPM--DHLHIFPYNSNYLKLSRLEFNIL-----SQYT 117

Query: 472 SKESKRVAFFGSGPLPLSTIVMAMGNYFGEHAVFDNYDMDSSANDLARNLVEPHEMLANK 651
           SK  K +AF GSGPLPL++I +A+ +   +   F NYD+D+ AN +A  LV  H  L+ +
Sbjct: 118 SKTPKSLAFVGSGPLPLTSITLALNHL--KFTNFHNYDIDTGANSMALGLVSSHPDLSKR 175

Query: 652 MFFHD 666
           MFFH+
Sbjct: 176 MFFHN 180


>ref|XP_004984619.1| PREDICTED: nicotianamine synthase 2-like [Setaria italica]
          Length = 605

 Score =  132 bits (332), Expect = 1e-28
 Identities = 85/185 (45%), Positives = 109/185 (58%), Gaps = 1/185 (0%)
 Frame = +1

Query: 112 VVERVRGLYGKICKLQGSLKPSQNVNALFGELVSVCGGAQGRNVRVDKL-PSADRVWRFH 288
           +V ++ GL+  I KL  SL PS  V+ALF +LV  C       V V KL P A R+ R  
Sbjct: 11  LVHKIAGLHAAIAKLP-SLSPSPQVDALFTDLVMAC--VPPSPVDVTKLDPEAQRM-REE 66

Query: 289 LIQLCSESEGELERHYSRLLGKLERPCSPESLRMFPYYDNYVKLSKLEYTTLCMAAPEMS 468
           LI+LCS++EG LE HYS +L   + P   + L  FPY+ NY+ LSKLEY  L    P ++
Sbjct: 67  LIRLCSDAEGHLEAHYSDMLNAFDNPL--DHLGRFPYFSNYINLSKLEYDLLVRYIPGLA 124

Query: 469 ASKESKRVAFFGSGPLPLSTIVMAMGNYFGEHAVFDNYDMDSSANDLARNLVEPHEMLAN 648
            S    RVAF GSGPLP S++V+A  +    + VFDNYD   +AND AR LV   E L  
Sbjct: 125 PS----RVAFVGSGPLPFSSLVLAARHL--PNTVFDNYDRSGAANDRARKLVRADEDLRK 178

Query: 649 KMFFH 663
           +M FH
Sbjct: 179 RMAFH 183



 Score =  130 bits (326), Expect = 5e-28
 Identities = 81/184 (44%), Positives = 106/184 (57%)
 Frame = +1

Query: 112 VVERVRGLYGKICKLQGSLKPSQNVNALFGELVSVCGGAQGRNVRVDKLPSADRVWRFHL 291
           +V+++  L+  I KL  SL PS +V+ LF ELV  C       V V KL    +  R  L
Sbjct: 289 LVQKITSLHAAITKLP-SLSPSPDVDRLFTELVMAC--VPPSPVDVTKLAPDAQKMREEL 345

Query: 292 IQLCSESEGELERHYSRLLGKLERPCSPESLRMFPYYDNYVKLSKLEYTTLCMAAPEMSA 471
           I+LCS++EG LE HYS +L   + P   + L  FPY+ NY+ LSKLEY  L    P ++ 
Sbjct: 346 IRLCSDAEGHLEAHYSDMLNAFDNPL--DHLSRFPYFSNYINLSKLEYDLLVRYIPGLAP 403

Query: 472 SKESKRVAFFGSGPLPLSTIVMAMGNYFGEHAVFDNYDMDSSANDLARNLVEPHEMLANK 651
           S    RVAF GSGPLP S++V+A  +    + VFDNYD   +AND AR LV   E L  +
Sbjct: 404 S----RVAFVGSGPLPFSSLVLAARHL--PNTVFDNYDRSGAANDRARKLVRADEDLRKR 457

Query: 652 MFFH 663
           M FH
Sbjct: 458 MTFH 461


>gb|AFH08366.1| nicotianamine synthase 2 [Arabidopsis halleri subsp. halleri]
          Length = 320

 Score =  132 bits (331), Expect = 1e-28
 Identities = 81/186 (43%), Positives = 115/186 (61%), Gaps = 2/186 (1%)
 Frame = +1

Query: 112 VVERVRGLYGKICKLQGSLKPSQNVNALFGELVSVCGGAQGRNVRVDKLPSAD-RVWRFH 288
           VV+++  LY +I  L+ SLKPS+NV+ LFG+LVS C      N+ V ++     +  R H
Sbjct: 8   VVKQIIDLYNQISNLE-SLKPSKNVDTLFGQLVSTCLPTD-TNIDVTEIHDEKVKDARSH 65

Query: 289 LIQLCSESEGELERHYSRLLGKLE-RPCSPESLRMFPYYDNYVKLSKLEYTTLCMAAPEM 465
           LI+LC E+EG LE+H+S +LG  E  P +   L +FPYY+NY+KLSKLE+  L      +
Sbjct: 66  LIKLCGEAEGYLEQHFSTILGSFEDNPLN--HLHIFPYYNNYIKLSKLEFDLLAQHTTHV 123

Query: 466 SASKESKRVAFFGSGPLPLSTIVMAMGNYFGEHAVFDNYDMDSSANDLARNLVEPHEMLA 645
                  +VAF GSGP+PL++IV+A   +   +  F N+D+DS AN LA NLV     L+
Sbjct: 124 PT-----KVAFIGSGPMPLTSIVLA--KFHLPNTTFHNFDIDSHANTLASNLVSRDSDLS 176

Query: 646 NKMFFH 663
            +M FH
Sbjct: 177 KRMVFH 182


>ref|XP_006392427.1| hypothetical protein EUTSA_v10024166mg [Eutrema salsugineum]
           gi|557088933|gb|ESQ29713.1| hypothetical protein
           EUTSA_v10024166mg [Eutrema salsugineum]
          Length = 325

 Score =  131 bits (330), Expect = 2e-28
 Identities = 81/186 (43%), Positives = 115/186 (61%), Gaps = 1/186 (0%)
 Frame = +1

Query: 109 EVVERVRGLYGKICKLQGSLKPSQNVNALFGELVSVCGGAQGRNVRVDKLPSADRVWRFH 288
           ++V  +  LY KI KL+ SLKPS++V+ LF +LVS C      N+ V K+  + +  R  
Sbjct: 8   QLVNTICDLYEKISKLE-SLKPSEDVDTLFKQLVSTCI-PPNHNIDVTKMSESIQEMRSS 65

Query: 289 LIQLCSESEGELERHYSRLLGKLERPCSP-ESLRMFPYYDNYVKLSKLEYTTLCMAAPEM 465
           LI+LC E+EG LE H+S +L   E   +P   L +FPYY+NY+KLSKLEY  L     + 
Sbjct: 66  LIKLCGEAEGHLEHHFSSVLTSFED--NPLHHLNLFPYYNNYLKLSKLEYDLL----EQN 119

Query: 466 SASKESKRVAFFGSGPLPLSTIVMAMGNYFGEHAVFDNYDMDSSANDLARNLVEPHEMLA 645
                 K VAF GSGPLPL++IV+A  ++  + ++F N+D+D SAN +A  LV     L+
Sbjct: 120 LNGSVPKTVAFVGSGPLPLTSIVLA--SFHLKDSIFHNFDVDPSANSVAARLVSSDPDLS 177

Query: 646 NKMFFH 663
            +MFFH
Sbjct: 178 QRMFFH 183


>sp|Q9XFB6.1|NAS8_HORVU RecName: Full=Nicotianamine synthase 8; AltName:
           Full=S-adenosyl-L-methionine:S-adenosyl-L-methionine:S-
           adenosyl-methionine 3-amino-3-carboxypropyltransferase 8
           gi|4894910|gb|AAD32650.1|AF136941_1 nicotianamine
           synthase 1 [Hordeum vulgare subsp. vulgare]
          Length = 329

 Score =  131 bits (330), Expect = 2e-28
 Identities = 82/184 (44%), Positives = 108/184 (58%)
 Frame = +1

Query: 112 VVERVRGLYGKICKLQGSLKPSQNVNALFGELVSVCGGAQGRNVRVDKLPSADRVWRFHL 291
           +V+++ GL+  I KL  SL PS +V+ALF +LV+ C       V V KL S  +  R  L
Sbjct: 11  LVQKITGLHAAIAKLP-SLSPSPDVDALFTDLVTAC--VPPSPVDVTKLGSEAQEMREGL 67

Query: 292 IQLCSESEGELERHYSRLLGKLERPCSPESLRMFPYYDNYVKLSKLEYTTLCMAAPEMSA 471
           I+LCSE+EG+LE HYS +L   + P   + L MFPYY NY+ LSKLEY  L    P    
Sbjct: 68  IRLCSEAEGKLEAHYSDMLAAFDNPL--DHLGMFPYYSNYINLSKLEYELLARYVP---G 122

Query: 472 SKESKRVAFFGSGPLPLSTIVMAMGNYFGEHAVFDNYDMDSSANDLARNLVEPHEMLANK 651
                RVAF GSGPLP S+ V+A  +     A+FDNYD+ S+AND A  L    + +  +
Sbjct: 123 RHRPARVAFIGSGPLPFSSYVLAARHL--PDAMFDNYDLCSAANDRASKLFRADKDVGAR 180

Query: 652 MFFH 663
           M FH
Sbjct: 181 MSFH 184


>gb|EXB98412.1| Nicotianamine synthase [Morus notabilis]
          Length = 349

 Score =  130 bits (327), Expect = 4e-28
 Identities = 79/186 (42%), Positives = 114/186 (61%), Gaps = 2/186 (1%)
 Frame = +1

Query: 112 VVERVRGLYGKICKLQGSLKPSQNVNALFGELVSVCGGAQGRNVRVDKLPSADRVWRFHL 291
           ++ +V  LY +I +L  SLKPS++V+ LF +LV  C       + V+KL    +  R +L
Sbjct: 9   LITKVCQLYDQISRLS-SLKPSKDVDTLFTQLVLTC--IPPNPIDVNKLCKPIQNIRSNL 65

Query: 292 IQLCSESEGELERHYSRLLGKLERPCSPESLRMFPYYDNYVKLSKLEYTTLC--MAAPEM 465
           I+LC E+EG LE H+S LL   + P   ++L +FPYY NY+KLS+LEY  L   +    +
Sbjct: 66  IRLCGEAEGHLESHFSTLLASYQNPL--QNLNIFPYYSNYLKLSQLEYNILSQHLTKTNI 123

Query: 466 SASKESKRVAFFGSGPLPLSTIVMAMGNYFGEHAVFDNYDMDSSANDLARNLVEPHEMLA 645
             SK + ++AF GSGPLPL++IV+A  +   + A F NYD+D  AN  A  LV     L+
Sbjct: 124 DDSKTALKIAFIGSGPLPLTSIVLASNHL--KTASFHNYDIDPLANSKAEALVSSDPDLS 181

Query: 646 NKMFFH 663
           N+MFFH
Sbjct: 182 NRMFFH 187


>ref|XP_006382395.1| hypothetical protein POPTR_0005s01730g [Populus trichocarpa]
           gi|550337755|gb|ERP60192.1| hypothetical protein
           POPTR_0005s01730g [Populus trichocarpa]
          Length = 269

 Score =  130 bits (327), Expect = 4e-28
 Identities = 80/184 (43%), Positives = 110/184 (59%)
 Frame = +1

Query: 112 VVERVRGLYGKICKLQGSLKPSQNVNALFGELVSVCGGAQGRNVRVDKLPSADRVWRFHL 291
           +V++V  LY +I  L+ SLKPS++V+ LF +LV  C       + V+KL    +  R  L
Sbjct: 8   LVQKVCDLYEQISSLE-SLKPSKDVDMLFTQLVLTC--IPPNPIDVNKLCKKIQEMRSKL 64

Query: 292 IQLCSESEGELERHYSRLLGKLERPCSPESLRMFPYYDNYVKLSKLEYTTLCMAAPEMSA 471
           I+LC E+EG LE H+S +LG  E P   + L +FPYY NY+KLS LE+  L     +   
Sbjct: 65  IRLCGEAEGHLESHFSAILGSYENPL--DHLNVFPYYSNYLKLSHLEFNIL-----KQHC 117

Query: 472 SKESKRVAFFGSGPLPLSTIVMAMGNYFGEHAVFDNYDMDSSANDLARNLVEPHEMLANK 651
           +    +VAF GSGPLPLS+IV+A  +       F NYD+D SAN  A  LV  H  L+++
Sbjct: 118 TNVPSKVAFVGSGPLPLSSIVLACNHLTA--TSFHNYDIDPSANSKALQLVTSHPDLSSR 175

Query: 652 MFFH 663
           MFFH
Sbjct: 176 MFFH 179