BLASTX nr result
ID: Ephedra28_contig00003836
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Ephedra28_contig00003836
(1174 letters)
Database: ./nr
37,332,560 sequences; 13,225,080,153 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|ADZ45513.1| alpha-humulene synthase [Picea glauca] 149 2e-33
gb|ABV44452.1| caryophyllene/humulene synthase [Pinus sylvestris] 142 2e-31
sp|O64404.1|TPSD4_ABIGR RecName: Full=Delta-selinene synthase; A... 142 3e-31
gb|AAK83561.1| delta-selinene synthase [Abies grandis] 140 1e-30
gb|ADL14246.1| pimara-8(14),15-diene synthase [Taiwania cryptome... 138 5e-30
gb|ABA86249.1| delta-selinene-like synthase [Picea sitchensis] 137 6e-30
gb|ACN40827.1| unknown [Picea sitchensis] 131 5e-28
gb|AAR13861.1| taxa-4(5),11(12)-diene synthase [synthetic constr... 129 2e-27
gb|ABV44453.1| 1(10),5-germacradien-4-ol synthase [Pinus sylvest... 125 4e-26
gb|ACN39765.1| unknown [Picea sitchensis] gi|325930026|gb|ADZ455... 124 1e-25
gb|ABV44454.1| longifolene synthase [Pinus sylvestris] 123 1e-25
gb|AAY16197.1| taxadiene synthase [Taxus mairei] 122 3e-25
sp|Q93YA3.1|TASY_TAXBA RecName: Full=Taxadiene synthase; AltName... 122 4e-25
sp|Q41594.1|TASY_TAXBR RecName: Full=Taxadiene synthase; AltName... 122 4e-25
gb|AAK83566.1| taxadiene synthase [Taxus brevifolia] 122 4e-25
gb|ABC25488.1| taxadiene synthase [Taxus cuspidata] 122 4e-25
gb|AAS18603.1| taxadiene synthase [Taxus x media] 122 4e-25
pdb|3P5P|A Chain A, Crystal Structure Of Taxadiene Synthase From... 122 4e-25
gb|AAR13860.1| taxa-4(5),11(12)-diene synthase [Taxus canadensis] 122 4e-25
gb|AAR02861.1| taxadiene synthase [Taxus baccata] 122 4e-25
>gb|ADZ45513.1| alpha-humulene synthase [Picea glauca]
Length = 575
Score = 149 bits (376), Expect = 2e-33
Identities = 81/288 (28%), Positives = 143/288 (49%), Gaps = 3/288 (1%)
Frame = -2
Query: 1167 EIPKFEFLRHRPIESYYYSMLSIFEPDYKDARIIQAKLTMFCYCMDDFSDNYASSQELQD 988
EI + F R R +E Y++++ IFEP++ +RI AK+T +DD D + + EL+
Sbjct: 280 EIAQLNFYRRRHVELYFWAVTCIFEPEFSPSRIAFAKITTVGAVLDDLYDTHGTLDELKT 339
Query: 987 IIEALESWDYSTNGITKFRGDLEKVVLYLFKIMGEICSTASERQGRDMKDYIKALVVEYA 808
I EA+ WD S I +++ + F E+ ++QGRDM +KA Y
Sbjct: 340 ITEAVRRWDLSL--IDDLPNNIKIACQFFFNTANELAVEVVKKQGRDMTALLKATWQRYV 397
Query: 807 KAHLRENVWNERGEIPTLDEYLEINHRSXXXXXXXXXXXXXXASCVDEKFCQRFLRACK- 631
+++L+E W E +P+ +EY++ S + ++ L K
Sbjct: 398 ESYLQEAEWIETRHVPSFNEYIKNALVSSGMCIVNLIPLLLLGQLLANNIVEQILSPSKI 457
Query: 630 --LGDIMGRLVDDGRELLENDTSETTVSVITCYLKQNPGSSMSDALSYARDLFKNYYREI 457
L ++ RL+DD R+ + S++ CY+K NP S++ +AL++ + + E+
Sbjct: 458 QELSELTIRLIDDIRDFEDEKERGEIASIVECYMKDNPDSTLENALNHIKGILHVSLEEL 517
Query: 456 NYWYLMEHDIPMVCRSYCFNVIRSSLFFYTVVDGFSKCNDVIKDQIKK 313
N+ ++ + +P+ C+ + FN++R F Y DG S N +KDQI K
Sbjct: 518 NWEFMKDDSVPLCCKKFTFNIVRGLQFLYKYGDGISISNKEVKDQIFK 565
>gb|ABV44452.1| caryophyllene/humulene synthase [Pinus sylvestris]
Length = 575
Score = 142 bits (359), Expect = 2e-31
Identities = 82/288 (28%), Positives = 138/288 (47%), Gaps = 3/288 (1%)
Frame = -2
Query: 1167 EIPKFEFLRHRPIESYYYSMLSIFEPDYKDARIIQAKLTMFCYCMDDFSDNYASSQELQD 988
E+ + F R R +E Y+++++SIFEP++ +RI AK+T +DD D Y EL+
Sbjct: 280 EVAQVNFYRRRHMELYFWAVISIFEPEFSQSRIAFAKVTTVGTVLDDLYDTYGMLDELKT 339
Query: 987 IIEALESWDYSTNGITKFRGDLEKVVLYLFKIMGEICSTASERQGRDMKDYIKALVVEYA 808
I E + WD S I ++ + + F E+ + +QG D +K V Y
Sbjct: 340 ITEGVRRWDISL--IDDLPEKIKIAIQFFFNTANELAAEVVSKQGPDTSAILKDTWVRYL 397
Query: 807 KAHLRENVWNERGEIPTLDEYLEINHRSXXXXXXXXXXXXXXASCVDEKFCQRFLRACK- 631
+++L+E W G +PT EY++ S + Q+ K
Sbjct: 398 ESYLQEAEWITTGCVPTFSEYIKNAVASSGMCIVNLIPLLLMGQPLPNNILQQIHSPSKI 457
Query: 630 --LGDIMGRLVDDGRELLENDTSETTVSVITCYLKQNPGSSMSDALSYARDLFKNYYREI 457
L ++ RL+DD R+ + S+I CY+K NP S++ +AL+ + + + E+
Sbjct: 458 QELSELTIRLIDDLRDFEDEKERGEMASIIECYIKDNPDSTVGNALNRIKGILQLSLEEM 517
Query: 456 NYWYLMEHDIPMVCRSYCFNVIRSSLFFYTVVDGFSKCNDVIKDQIKK 313
N +L + +P+ C+ + FN+ R F Y DG S N+ +KDQI K
Sbjct: 518 NREFLKQDSVPLCCKKFTFNITRGLQFLYKYGDGISISNNEVKDQIFK 565
>sp|O64404.1|TPSD4_ABIGR RecName: Full=Delta-selinene synthase; AltName: Full=Agfdsel1
gi|2961460|gb|AAC05727.1| d-selinene synthase [Abies
grandis]
Length = 581
Score = 142 bits (357), Expect = 3e-31
Identities = 79/288 (27%), Positives = 141/288 (48%), Gaps = 4/288 (1%)
Frame = -2
Query: 1164 IPKFEFLRHRPIESYYYSMLSIFEPDYKDARIIQAKLTMFCYCMDDFSDNYASSQELQDI 985
I + F R R +E Y + ++ IFEP++ ++RI AK + C +DD D +A+ E++ +
Sbjct: 287 ISQLNFYRKRHVEYYSWVVMCIFEPEFSESRIAFAKTAILCTVLDDLYDTHATLHEIKIM 346
Query: 984 IEALESWDYSTNGITKFRGDLEKVVL-YLFKIMGEICSTASERQGRDMKDYIKALVVEYA 808
E + WD S +T D K+ + F + E+ +RQGRDM +K Y
Sbjct: 347 TEGVRRWDLS---LTDDLPDYIKIAFQFFFNTVNELIVEIVKRQGRDMTTIVKDCWKRYI 403
Query: 807 KAHLRENVWNERGEIPTLDEYLEINHRSXXXXXXXXXXXXXXASCVDEKFCQRFLRACKL 628
+++L+E W G IPT +EY++ S + + ++ K+
Sbjct: 404 ESYLQEAEWIATGHIPTFNEYIKNGMASSGMCILNLNPLLLLDKLLPDNILEQIHSPSKI 463
Query: 627 GDIM---GRLVDDGRELLENDTSETTVSVITCYLKQNPGSSMSDALSYARDLFKNYYREI 457
D++ GR+ DD ++ + S + CY+K+NP S++ +AL++ + + E
Sbjct: 464 LDLLELTGRIADDLKDFEDEKERGEMASSLQCYMKENPESTVENALNHIKGILNRSLEEF 523
Query: 456 NYWYLMEHDIPMVCRSYCFNVIRSSLFFYTVVDGFSKCNDVIKDQIKK 313
N+ ++ + +PM C+ + FN+ R F Y DG + +KDQI K
Sbjct: 524 NWEFMKQDSVPMCCKKFTFNIGRGLQFIYKYRDGLYISDKEVKDQIFK 571
>gb|AAK83561.1| delta-selinene synthase [Abies grandis]
Length = 579
Score = 140 bits (352), Expect = 1e-30
Identities = 77/287 (26%), Positives = 139/287 (48%), Gaps = 3/287 (1%)
Frame = -2
Query: 1164 IPKFEFLRHRPIESYYYSMLSIFEPDYKDARIIQAKLTMFCYCMDDFSDNYASSQELQDI 985
I F R+R +E Y+++++ IFEP++ ++RI AK C +DD D +A+ EL+ +
Sbjct: 285 ISDLNFYRNRHVEYYFWAVIGIFEPEFSESRIAFAKTATVCTILDDLYDTHATLDELKIM 344
Query: 984 IEALESWDYSTNGITKFRGDLEKVVLYLFKIMGEICSTASERQGRDMKDYIKALVVEYAK 805
E + WD S I ++ + F I E+ ++QGRDM +K Y +
Sbjct: 345 TEGVRRWDLSL--IHDLPDYIKIAFQFFFNIADELIFEIVKQQGRDMTAIVKDCWKRYIE 402
Query: 804 AHLRENVWNERGEIPTLDEYLEINHRSXXXXXXXXXXXXXXASCVDEKFCQRFLRACK-- 631
++L+E W G +PT +EY++ S + + ++ K
Sbjct: 403 SYLQEAEWIASGHMPTFNEYIKNGLASSGLCIVNLNPLLLLGKLLPDNILEQIHSPSKIQ 462
Query: 630 -LGDIMGRLVDDGRELLENDTSETTVSVITCYLKQNPGSSMSDALSYARDLFKNYYREIN 454
L ++ GR+ DD ++ + S + CY+K+NP S++ +AL++ + + E N
Sbjct: 463 ELSELTGRITDDLKDFEDEKERGEMASSLQCYMKENPESTVENALNHIKGILNRSLEEFN 522
Query: 453 YWYLMEHDIPMVCRSYCFNVIRSSLFFYTVVDGFSKCNDVIKDQIKK 313
+ ++ + +PM C+ + FN+ R F Y DG + +KDQI K
Sbjct: 523 WEFMKQDSVPMCCKKFTFNIGRGLQFLYKYRDGLYISDKEVKDQIFK 569
>gb|ADL14246.1| pimara-8(14),15-diene synthase [Taiwania cryptomerioides]
Length = 851
Score = 138 bits (347), Expect = 5e-30
Identities = 87/287 (30%), Positives = 135/287 (47%), Gaps = 3/287 (1%)
Frame = -2
Query: 1161 PKFEFLRHRPIESYYYSMLSIFEPDYKDARIIQAKLTMFCYCMDDFSDNYASSQELQDII 982
P+ EF RHR + Y+ + +FEP Y D R+ AK + DD D YA+ + +
Sbjct: 561 PQLEFTRHREVAIYWTASTVMFEPQYTDCRLAYAKAGIMAVITDDLYDTYATLDQAKLFN 620
Query: 981 EALESWDYSTNGITKFRGDLEKVVLYLFKIMGEICSTASERQGRDMKDYIKALVVEYAKA 802
EA E WD S I D+ V + L+ + +I A E QGRD+ Y++ ++
Sbjct: 621 EAFERWDPSQ--IDHLPEDMRIVFMGLYNTLTDISERAREVQGRDVLPYLRQKWLDLFFR 678
Query: 801 HLRENVWNERGEIPTLDEYLEINHRSXXXXXXXXXXXXXXASCVDEKFCQRF-LRA--CK 631
+ +E W ER P+LD+Y E S + + Q+F RA
Sbjct: 679 YTKETDWMERHYSPSLDQYWENAVESIALGVTTLTPIFSTGDVLPDHILQKFDFRAEFLN 738
Query: 630 LGDIMGRLVDDGRELLENDTSETTVSVITCYLKQNPGSSMSDALSYARDLFKNYYREINY 451
L + GRL++D R E S + CY+ +PG + +AL+Y + ++ ++NY
Sbjct: 739 LVSLTGRLINDVRTFQEERDRGELASCVQCYINDHPGCTEEEALNYLYGVNEDALTKLNY 798
Query: 450 WYLMEHDIPMVCRSYCFNVIRSSLFFYTVVDGFSKCNDVIKDQIKKT 310
+LM +IP R+ FN R FY +DGF + +K+ IKKT
Sbjct: 799 HFLMRAEIPKSFRTVLFNTARVMQLFYRNIDGFLNAAEEMKEFIKKT 845
>gb|ABA86249.1| delta-selinene-like synthase [Picea sitchensis]
Length = 575
Score = 137 bits (346), Expect = 6e-30
Identities = 77/288 (26%), Positives = 135/288 (46%), Gaps = 3/288 (1%)
Frame = -2
Query: 1167 EIPKFEFLRHRPIESYYYSMLSIFEPDYKDARIIQAKLTMFCYCMDDFSDNYASSQELQD 988
EI + F R R +E Y++ ++ IFEP++ +RI AK+ +DD D + EL+
Sbjct: 280 EIAQLNFYRKRHVEFYFWVVICIFEPEFSQSRIAFAKICTVATVLDDLYDTHGMLDELKT 339
Query: 987 IIEALESWDYSTNGITKFRGDLEKVVLYLFKIMGEICSTASERQGRDMKDYIKALVVEYA 808
+ E + WD I +++ + F E+ ++QGRDM +KA Y
Sbjct: 340 VTEGVSRWDLPL--IDDLPDNIKIAFQFFFNTANELAVEVVKKQGRDMIALLKANWQRYV 397
Query: 807 KAHLRENVWNERGEIPTLDEYLEINHRSXXXXXXXXXXXXXXASCVDEKFCQRFLRACK- 631
+++L+E W +P+ DEY++ S + ++ K
Sbjct: 398 ESYLQEAEWIATRHVPSFDEYIKNARASSGMCIGNLIPLLLLGQLLANNIVEQIHSPSKI 457
Query: 630 --LGDIMGRLVDDGRELLENDTSETTVSVITCYLKQNPGSSMSDALSYARDLFKNYYREI 457
L ++ RL+DD R+ + S I CY+K NP S++ +AL++ + E+
Sbjct: 458 QELSELTIRLIDDIRDFEDEKERGEIASAIECYMKDNPDSTVENALNHLEGILHLSLEEL 517
Query: 456 NYWYLMEHDIPMVCRSYCFNVIRSSLFFYTVVDGFSKCNDVIKDQIKK 313
N+ ++ + +P+ C+ + FN++R F Y DG S N +KDQI K
Sbjct: 518 NWEFIKQDTVPLCCKKFTFNIVRGLQFVYKYGDGLSISNKEVKDQIFK 565
>gb|ACN40827.1| unknown [Picea sitchensis]
Length = 654
Score = 131 bits (330), Expect = 5e-28
Identities = 76/288 (26%), Positives = 137/288 (47%), Gaps = 4/288 (1%)
Frame = -2
Query: 1164 IPKFEFLRHRPIESYYYSMLSIFEPDYKDARIIQAKLTMFCYCMDDFSDNYASSQELQDI 985
+P+ F R R +E Y++ + FEP++ +RI K+ +DD D + + E++
Sbjct: 361 LPQLGFYRKRHVELYFWVVTGTFEPEFSSSRIAFTKIATLATVLDDLYDTHGTLDEVKIF 420
Query: 984 IEALESWDYSTNGITKFRGDLEKVVLYLFKIMGEICSTASERQGRDMKDYIKALVVE-YA 808
E + WD S G + ++K+ + K E+ ++QGRDM+ YI+ E Y
Sbjct: 421 TEGVRRWDNSLIG--RLPDHIKKIFEFFMKTSNEMNDEVEKKQGRDMRAYIRKNGWERYL 478
Query: 807 KAHLRENVWNERGEIPTLDEYLEINHRSXXXXXXXXXXXXXXASCVDEKFCQRFLRACKL 628
+++L+E W G +P+ +EYL+ S + E ++ K+
Sbjct: 479 ESYLQEAEWIAAGYVPSFNEYLKNGLASSGSCVLNLIPLLLMGQILPEDILEQIFFPSKI 538
Query: 627 GDIMG---RLVDDGRELLENDTSETTVSVITCYLKQNPGSSMSDALSYARDLFKNYYREI 457
+++ RL DD + + S + CYLK NP S+ DAL++ + + E+
Sbjct: 539 HELLEMTIRLKDDITDFEQEKERGEIASSLECYLKDNPESTREDALNHIKGILDLSVSEL 598
Query: 456 NYWYLMEHDIPMVCRSYCFNVIRSSLFFYTVVDGFSKCNDVIKDQIKK 313
N+ +L ++P+ C+ + FN R F + DG S ++ +KDQI K
Sbjct: 599 NWEFLKHDNVPLCCKKFTFNFARGMHFLFKYKDGVSISDNEVKDQILK 646
>gb|AAR13861.1| taxa-4(5),11(12)-diene synthase [synthetic construct]
Length = 861
Score = 129 bits (324), Expect = 2e-27
Identities = 84/283 (29%), Positives = 132/283 (46%), Gaps = 4/283 (1%)
Frame = -2
Query: 1149 FLRHRPIESYYYSMLSIFEPDYKDARIIQAKLTMFCYCMDDFSDNYASSQELQDIIEALE 970
F RHR E Y+ S + FEP+Y RI K+ DD +D +A+ EL+D + ++
Sbjct: 575 FTRHRVAEVYFSS--ATFEPEYSSTRIAFTKIGCLQVLFDDMADIFATLDELRDFTKGVK 632
Query: 969 SWDYSTNGITKFRGDLEKVVLYLFKIMGEICSTASERQGRDMKDYIKALVVEYAKAHLRE 790
WD T+ I K ++ F +M E+ + S QGRDM ++I+ Y +++E
Sbjct: 633 RWD--TSLIHKLPECMQTCFRVWFNLMEEVNNDVSNVQGRDMLNHIRKPWELYFNCYVQE 690
Query: 789 NVWNERGEIPTLDEYLEINHRSXXXXXXXXXXXXXXASCVDEKFCQRFLRACKLGDIMG- 613
W + G IPTL+EYL+ S V + ++ L +++
Sbjct: 691 REWLDAGYIPTLEEYLKTYSISVGLGPCTLQPILLMGDIVKDDVVEKVHYPSNLFELVSL 750
Query: 612 --RLVDDGRELLENDTSETTVSVITCYLKQNPGSSMSDALSYARDLFKNYYREINYWYLM 439
RL +D + S I CY+K NPGS+ DA+ Y + +E + Y
Sbjct: 751 SWRLTNDTKTYQAEKARGQQASGIACYMKDNPGSTEEDAIKYICGVVDRALKEACFEYFK 810
Query: 438 -EHDIPMVCRSYCFNVIRSSLFFYTVVDGFSKCNDVIKDQIKK 313
D+PM C+S+ FN+ FY +DG+ N+ IKD I+K
Sbjct: 811 PADDVPMSCKSFIFNLRLCVQIFYKFIDGYGIANEEIKDYIRK 853
>gb|ABV44453.1| 1(10),5-germacradien-4-ol synthase [Pinus sylvestris]
Length = 625
Score = 125 bits (313), Expect = 4e-26
Identities = 70/288 (24%), Positives = 141/288 (48%), Gaps = 4/288 (1%)
Frame = -2
Query: 1164 IPKFEFLRHRPIESYYYSMLSIFEPDYKDARIIQAKLTMFCYCMDDFSDNYASSQELQDI 985
+P+ +F R R +E Y++++L FEP+++ +RI KL+ +DD D + + E++
Sbjct: 332 LPQLDFYRKRHVELYFWAVLGTFEPEFRSSRIAFTKLSTVMTVIDDLYDTHGTLDEIKIF 391
Query: 984 IEALESWDYSTNGITKFRGDLEKVVLYLFKIMGEICSTASERQGRDMKDYIKALVVE-YA 808
E + WD T+ I++ ++K+ + K E + ++QGRDM YI+ E Y
Sbjct: 392 TEGVRRWD--TSLISRLPDHIQKIFEFFMKTSNEWTAEVEKKQGRDMAAYIRKNGWERYV 449
Query: 807 KAHLRENVWNERGEIPTLDEYLEINHRSXXXXXXXXXXXXXXASCVDEKFCQRFLRACKL 628
+++L+E W G +P+ +EY + S + + ++ + K+
Sbjct: 450 ESYLQEGEWMAAGYVPSFNEYYKNGLASSGMCVLNLIPLLLMDQILPDDILKQIVYPSKI 509
Query: 627 GDIMG---RLVDDGRELLENDTSETTVSVITCYLKQNPGSSMSDALSYARDLFKNYYREI 457
+++ R+ DD + + S CY+K NP + DAL++ + + ++
Sbjct: 510 HELLELTIRVKDDITDFEKEKEHGQVASCSECYMKDNPECTREDALNHMKGILDLSVSQL 569
Query: 456 NYWYLMEHDIPMVCRSYCFNVIRSSLFFYTVVDGFSKCNDVIKDQIKK 313
N+ +L ++P+ + + FN+ R F + DG + ++ +KDQI K
Sbjct: 570 NWEFLKHDNVPLCYKRFTFNLARGMHFLFKYNDGITLSDNEVKDQIFK 617
>gb|ACN39765.1| unknown [Picea sitchensis] gi|325930026|gb|ADZ45516.1|
alpha-longipinene synthase [Picea sitchensis]
Length = 579
Score = 124 bits (310), Expect = 1e-25
Identities = 77/292 (26%), Positives = 132/292 (45%), Gaps = 5/292 (1%)
Frame = -2
Query: 1173 REEIPKFEFLRHRPIESYYYSMLSIFEPDYKDARIIQAKLTMFCYCMDDFSDNYASSQEL 994
+ +I + F R R +E Y++ ++ FEP++ RI AK+ +DD D + + ++L
Sbjct: 285 QSDIAQQNFYRKRHVEFYFWVVIGTFEPEFSTCRITFAKIATLMTILDDLYDTHGTLEQL 344
Query: 993 QDIIEALESWDYSTNGITKFRGDLEKVVLYLF-KIMGEICSTASERQGRDMKDYIKALVV 817
+ E ++ WD S + D K+ F E+ + ++ Q RDM YI+
Sbjct: 345 KIFTEGVKRWDLS---LVDSLPDYIKITFEFFLNTSNELIAEVAKTQERDMSAYIRKTWE 401
Query: 816 EYAKAHLRENVWNERGEIPTLDEYLEINHRSXXXXXXXXXXXXXXASCVDEKFCQRFLRA 637
Y +A+++E W G +PT DEY++ S + + ++
Sbjct: 402 RYLEAYMQEAEWITAGHVPTFDEYMKNGISSSGMCILNLYSLLLMGQLLPDDVLEQIHSP 461
Query: 636 CKLGDIM---GRLVDDGRELLENDTSETTVSVITCYLKQNPGSSMSDALSYARDLFKNYY 466
K+ +++ RLVDD ++ N S I CY+K NP ++ DA ++ L
Sbjct: 462 SKIHELVELTARLVDDSKDFETNKVGGELASGIECYVKDNPECTLEDASNHLNGLLDLTV 521
Query: 465 REINYWYLMEHDIPMVC-RSYCFNVIRSSLFFYTVVDGFSKCNDVIKDQIKK 313
+E+N W + HD +C + + FNV R Y DGF N +K I K
Sbjct: 522 KELN-WEFVRHDSVALCFKKFAFNVARGLRLIYKYRDGFDVSNQEMKTHIFK 572
>gb|ABV44454.1| longifolene synthase [Pinus sylvestris]
Length = 580
Score = 123 bits (309), Expect = 1e-25
Identities = 80/293 (27%), Positives = 136/293 (46%), Gaps = 6/293 (2%)
Frame = -2
Query: 1173 REEIPKFEFLRHRPIESYYYSMLSIFEPDYKDARIIQAKLTMFCYCMDDFSDNYASSQEL 994
+ +I K F R R +E Y++ ++ FEP++ +RI AK+ +DD D + + ++L
Sbjct: 284 QSDIEKQNFYRKRHVEFYFWMVIGTFEPEFSSSRIAFAKIATLMTILDDLYDTHGTLEQL 343
Query: 993 QDIIEALESWDYSTNGITKFRGDLEKVVL-YLFKIMGEICSTASERQGRDMKDYI-KALV 820
+ EA++ WD S D K+ L + F E+ + ++ Q RDM YI KA
Sbjct: 344 KIFTEAVKRWDLSLQDRLP---DYIKITLEFFFNTSNELNAEVAKMQERDMSAYIRKAGW 400
Query: 819 VEYAKAHLRENVWNERGEIPTLDEYLEINHRSXXXXXXXXXXXXXXASCVDEKFCQRFLR 640
Y + +++E+ W +PT D+Y++ RS V + ++
Sbjct: 401 ERYIEGYMQESEWMAARHVPTFDDYMKNGKRSSGMCILNLYSLLLMGQLVPDNILEQIHL 460
Query: 639 ACKLGDIM---GRLVDDGRELLENDTSETTVSVITCYLKQNPGSSMSDALSYARDLFKNY 469
K+ +++ RLVDD ++ S CYLK+ P + DA+++ L
Sbjct: 461 PSKIHELVELTARLVDDSKDFQAKKDGGEFASGTECYLKEKPECTEEDAMNHLIGLLNLT 520
Query: 468 YREINYWYLMEHDIPMVC-RSYCFNVIRSSLFFYTVVDGFSKCNDVIKDQIKK 313
E+N W ++HD +C + + F V R F Y DGF N+ +K QI K
Sbjct: 521 AMELN-WEFVKHDGVALCLKKFVFEVARGLRFIYKYRDGFDYSNEEMKSQITK 572
>gb|AAY16197.1| taxadiene synthase [Taxus mairei]
Length = 862
Score = 122 bits (306), Expect = 3e-25
Identities = 80/283 (28%), Positives = 132/283 (46%), Gaps = 4/283 (1%)
Frame = -2
Query: 1149 FLRHRPIESYYYSMLSIFEPDYKDARIIQAKLTMFCYCMDDFSDNYASSQELQDIIEALE 970
F RHR E Y+ S + FEP+Y RI K+ DD +D +A+ EL+ E ++
Sbjct: 576 FTRHRVAEVYFSS--ATFEPEYSTTRIAFTKIGCLQVLFDDMADIFATLDELKSFTEGVK 633
Query: 969 SWDYSTNGITKFRGDLEKVVLYLFKIMGEICSTASERQGRDMKDYIKALVVEYAKAHLRE 790
WD T+ + + ++ FK+M E+ + + QGRDM +I+ Y +++E
Sbjct: 634 RWD--TSLLHEIPECMQTCFKVWFKLMEEVNNDVVKVQGRDMLAHIRKPWELYFNCYVQE 691
Query: 789 NVWNERGEIPTLDEYLEINHRSXXXXXXXXXXXXXXASCVDEKFCQRFLRACKLGDIMG- 613
W E G IPT +EYL+ S V + ++ + +++
Sbjct: 692 REWLEAGYIPTFEEYLKTYAISVGLGPCTLQPILLMGELVKDDVVEKVHYPSNMFELVSL 751
Query: 612 --RLVDDGRELLENDTSETTVSVITCYLKQNPGSSMSDALSYARDLFKNYYREINYWYLM 439
RL +D + S I CY+K NPG++ DA+ + + +E ++ Y
Sbjct: 752 SWRLTNDTKTYQAEKARGQQASGIACYMKDNPGATEEDAIKHICRVVDRALKEASFEYFK 811
Query: 438 -EHDIPMVCRSYCFNVIRSSLFFYTVVDGFSKCNDVIKDQIKK 313
+DIPM C+S+ FN+ FY +DG+ N+ IKD I+K
Sbjct: 812 PSNDIPMGCKSFIFNLRLCVQIFYKFIDGYGIANEEIKDYIRK 854
>sp|Q93YA3.1|TASY_TAXBA RecName: Full=Taxadiene synthase; AltName:
Full=Taxa-4(5),11(12)-diene synthase
gi|14571793|emb|CAC42773.1| taxadiene synthase [Taxus
baccata]
Length = 862
Score = 122 bits (305), Expect = 4e-25
Identities = 80/283 (28%), Positives = 132/283 (46%), Gaps = 4/283 (1%)
Frame = -2
Query: 1149 FLRHRPIESYYYSMLSIFEPDYKDARIIQAKLTMFCYCMDDFSDNYASSQELQDIIEALE 970
F RHR E Y+ S + FEP+Y RI K+ DD +D +A+ EL+ E ++
Sbjct: 576 FTRHRVAEVYFSS--ATFEPEYSATRIAFTKIGCLQVLFDDMADIFATLDELKSFTEGVK 633
Query: 969 SWDYSTNGITKFRGDLEKVVLYLFKIMGEICSTASERQGRDMKDYIKALVVEYAKAHLRE 790
WD T+ + + ++ FK+M E+ + + QGRDM +I+ Y +++E
Sbjct: 634 RWD--TSLLHEIPECMQTCFKVWFKLMEEVNNDVVKVQGRDMLAHIRKPWELYFNCYVQE 691
Query: 789 NVWNERGEIPTLDEYLEINHRSXXXXXXXXXXXXXXASCVDEKFCQRFLRACKLGDIMG- 613
W E G IPT +EYL+ S V + ++ + +++
Sbjct: 692 REWLEAGYIPTFEEYLKTYAISVGLGPCTLQPILLMGELVKDDVVEKVHYPSNMFELVSL 751
Query: 612 --RLVDDGRELLENDTSETTVSVITCYLKQNPGSSMSDALSYARDLFKNYYREINYWYLM 439
RL +D + S I CY+K NPG++ DA+ + + +E ++ Y
Sbjct: 752 SWRLTNDTKTYQAEKARGQQASGIACYMKDNPGATEEDAIKHICRVVDRALKEASFEYFK 811
Query: 438 -EHDIPMVCRSYCFNVIRSSLFFYTVVDGFSKCNDVIKDQIKK 313
+DIPM C+S+ FN+ FY +DG+ N+ IKD I+K
Sbjct: 812 PSNDIPMGCKSFIFNLRLCVQIFYKFIDGYGIANEEIKDYIRK 854
>sp|Q41594.1|TASY_TAXBR RecName: Full=Taxadiene synthase; AltName:
Full=Taxa-4(5),11(12)-diene synthase
gi|1354139|gb|AAC49310.1| taxadiene synthase [Taxus
brevifolia] gi|1589568|prf||2211347A taxadiene synthase
Length = 862
Score = 122 bits (305), Expect = 4e-25
Identities = 80/283 (28%), Positives = 132/283 (46%), Gaps = 4/283 (1%)
Frame = -2
Query: 1149 FLRHRPIESYYYSMLSIFEPDYKDARIIQAKLTMFCYCMDDFSDNYASSQELQDIIEALE 970
F RHR E Y+ S + FEP+Y RI K+ DD +D +A+ EL+ E ++
Sbjct: 576 FTRHRVAEVYFSS--ATFEPEYSATRIAFTKIGCLQVLFDDMADIFATLDELKSFTEGVK 633
Query: 969 SWDYSTNGITKFRGDLEKVVLYLFKIMGEICSTASERQGRDMKDYIKALVVEYAKAHLRE 790
WD T+ + + ++ FK+M E+ + + QGRDM +I+ Y +++E
Sbjct: 634 RWD--TSLLHEIPECMQTCFKVWFKLMEEVNNDVVKVQGRDMLAHIRKPWELYFNCYVQE 691
Query: 789 NVWNERGEIPTLDEYLEINHRSXXXXXXXXXXXXXXASCVDEKFCQRFLRACKLGDIMG- 613
W E G IPT +EYL+ S V + ++ + +++
Sbjct: 692 REWLEAGYIPTFEEYLKTYAISVGLGPCTLQPILLMGELVKDDVVEKVHYPSNMFELVSL 751
Query: 612 --RLVDDGRELLENDTSETTVSVITCYLKQNPGSSMSDALSYARDLFKNYYREINYWYLM 439
RL +D + S I CY+K NPG++ DA+ + + +E ++ Y
Sbjct: 752 SWRLTNDTKTYQAEKARGQQASGIACYMKDNPGATEEDAIKHICRVVDRALKEASFEYFK 811
Query: 438 -EHDIPMVCRSYCFNVIRSSLFFYTVVDGFSKCNDVIKDQIKK 313
+DIPM C+S+ FN+ FY +DG+ N+ IKD I+K
Sbjct: 812 PSNDIPMGCKSFIFNLRLCVQIFYKFIDGYGIANEEIKDYIRK 854
>gb|AAK83566.1| taxadiene synthase [Taxus brevifolia]
Length = 862
Score = 122 bits (305), Expect = 4e-25
Identities = 80/283 (28%), Positives = 132/283 (46%), Gaps = 4/283 (1%)
Frame = -2
Query: 1149 FLRHRPIESYYYSMLSIFEPDYKDARIIQAKLTMFCYCMDDFSDNYASSQELQDIIEALE 970
F RHR E Y+ S + FEP+Y RI K+ DD +D +A+ EL+ E ++
Sbjct: 576 FTRHRVAEVYFSS--ATFEPEYSATRIAFTKIGCLQVLFDDMADIFATLDELKSFTEGVK 633
Query: 969 SWDYSTNGITKFRGDLEKVVLYLFKIMGEICSTASERQGRDMKDYIKALVVEYAKAHLRE 790
WD T+ + + ++ FK+M E+ + + QGRDM +I+ Y +++E
Sbjct: 634 RWD--TSLLHEIPECMQTCFKVWFKLMEEVNNDVVKVQGRDMLAHIRKPWELYFNCYVQE 691
Query: 789 NVWNERGEIPTLDEYLEINHRSXXXXXXXXXXXXXXASCVDEKFCQRFLRACKLGDIMG- 613
W E G IPT +EYL+ S V + ++ + +++
Sbjct: 692 REWLEAGYIPTFEEYLKTYAISVGLGPCTLQPILLMGELVKDDVVEKVHYPSNMFELVSL 751
Query: 612 --RLVDDGRELLENDTSETTVSVITCYLKQNPGSSMSDALSYARDLFKNYYREINYWYLM 439
RL +D + S I CY+K NPG++ DA+ + + +E ++ Y
Sbjct: 752 SWRLTNDTKTYQAEKVRGQQASGIACYMKDNPGATEEDAIKHICRVVDRALKEASFEYFK 811
Query: 438 -EHDIPMVCRSYCFNVIRSSLFFYTVVDGFSKCNDVIKDQIKK 313
+DIPM C+S+ FN+ FY +DG+ N+ IKD I+K
Sbjct: 812 PSNDIPMGCKSFIFNLRLCVQIFYKFIDGYGIANEEIKDYIRK 854
>gb|ABC25488.1| taxadiene synthase [Taxus cuspidata]
Length = 862
Score = 122 bits (305), Expect = 4e-25
Identities = 80/283 (28%), Positives = 132/283 (46%), Gaps = 4/283 (1%)
Frame = -2
Query: 1149 FLRHRPIESYYYSMLSIFEPDYKDARIIQAKLTMFCYCMDDFSDNYASSQELQDIIEALE 970
F RHR E Y+ S + FEP+Y RI K+ DD +D +A+ EL+ E ++
Sbjct: 576 FTRHRVAEVYFSS--ATFEPEYSATRIAFTKIGCLQVLFDDMADIFATLDELKSFTEGVK 633
Query: 969 SWDYSTNGITKFRGDLEKVVLYLFKIMGEICSTASERQGRDMKDYIKALVVEYAKAHLRE 790
WD T+ + + ++ FK+M E+ + + QGRDM +I+ Y +++E
Sbjct: 634 RWD--TSLLHEIPECMQTCFKVWFKLMEEVNNDVVKVQGRDMLAHIRKPWELYFNCYVQE 691
Query: 789 NVWNERGEIPTLDEYLEINHRSXXXXXXXXXXXXXXASCVDEKFCQRFLRACKLGDIMG- 613
W E G IPT +EYL+ S V + ++ + +++
Sbjct: 692 REWLEAGYIPTFEEYLKTYAISVGLGPCTLQPILLMGELVKDDVVEKVHYPSNMFELVSL 751
Query: 612 --RLVDDGRELLENDTSETTVSVITCYLKQNPGSSMSDALSYARDLFKNYYREINYWYLM 439
RL +D + S I CY+K NPG++ DA+ + + +E ++ Y
Sbjct: 752 SWRLTNDTKTYQAEKARGQQASGIACYMKDNPGATEEDAIKHICRVVDRALKEASFEYFK 811
Query: 438 -EHDIPMVCRSYCFNVIRSSLFFYTVVDGFSKCNDVIKDQIKK 313
+DIPM C+S+ FN+ FY +DG+ N+ IKD I+K
Sbjct: 812 PSNDIPMGCKSFIFNLRLCVQIFYKFIDGYGIANEEIKDYIRK 854
>gb|AAS18603.1| taxadiene synthase [Taxus x media]
Length = 862
Score = 122 bits (305), Expect = 4e-25
Identities = 80/283 (28%), Positives = 132/283 (46%), Gaps = 4/283 (1%)
Frame = -2
Query: 1149 FLRHRPIESYYYSMLSIFEPDYKDARIIQAKLTMFCYCMDDFSDNYASSQELQDIIEALE 970
F RHR E Y+ S + FEP+Y RI K+ DD +D +A+ EL+ E ++
Sbjct: 576 FTRHRVAEVYFSS--ATFEPEYSATRIAFTKIGCLQVLFDDMADIFATLDELKSFTEGVK 633
Query: 969 SWDYSTNGITKFRGDLEKVVLYLFKIMGEICSTASERQGRDMKDYIKALVVEYAKAHLRE 790
WD T+ + + ++ FK+M E+ + + QGRDM +I+ Y +++E
Sbjct: 634 RWD--TSLLHEIPECMQTCFKVWFKLMEEVNNDVVKVQGRDMLAHIRKPWELYFNCYVQE 691
Query: 789 NVWNERGEIPTLDEYLEINHRSXXXXXXXXXXXXXXASCVDEKFCQRFLRACKLGDIMG- 613
W E G IPT +EYL+ S V + ++ + +++
Sbjct: 692 REWLEAGYIPTFEEYLKTYAISVGLGPCTLQPILLMGELVKDDVVEKVHYPSNMFELVSL 751
Query: 612 --RLVDDGRELLENDTSETTVSVITCYLKQNPGSSMSDALSYARDLFKNYYREINYWYLM 439
RL +D + S I CY+K NPG++ DA+ + + +E ++ Y
Sbjct: 752 SWRLTNDTKTYQAEKARGQQASGIACYMKDNPGATEEDAIKHICRVVDRALKEASFEYFK 811
Query: 438 -EHDIPMVCRSYCFNVIRSSLFFYTVVDGFSKCNDVIKDQIKK 313
+DIPM C+S+ FN+ FY +DG+ N+ IKD I+K
Sbjct: 812 PSNDIPMGCKSFIFNLRLCVQIFYKFIDGYGIANEEIKDYIRK 854
>pdb|3P5P|A Chain A, Crystal Structure Of Taxadiene Synthase From Pacific Yew
(Taxus Brevifolia) In Complex With Mg2+ And
13-Aza-13,14-Dihydrocopalyl Diphosphate
gi|315583585|pdb|3P5R|A Chain A, Crystal Structure Of
Taxadiene Synthase From Pacific Yew (Taxus Brevifolia) In
Complex With Mg2+ And 2-Fluorogeranylgeranyl Diphosphate
gi|315583586|pdb|3P5R|B Chain B, Crystal Structure Of
Taxadiene Synthase From Pacific Yew (Taxus Brevifolia) In
Complex With Mg2+ And 2-Fluorogeranylgeranyl Diphosphate
Length = 764
Score = 122 bits (305), Expect = 4e-25
Identities = 80/283 (28%), Positives = 132/283 (46%), Gaps = 4/283 (1%)
Frame = -2
Query: 1149 FLRHRPIESYYYSMLSIFEPDYKDARIIQAKLTMFCYCMDDFSDNYASSQELQDIIEALE 970
F RHR E Y+ S + FEP+Y RI K+ DD +D +A+ EL+ E ++
Sbjct: 470 FTRHRVAEVYFSS--ATFEPEYSATRIAFTKIGCLQVLFDDMADIFATLDELKSFTEGVK 527
Query: 969 SWDYSTNGITKFRGDLEKVVLYLFKIMGEICSTASERQGRDMKDYIKALVVEYAKAHLRE 790
WD T+ + + ++ FK+M E+ + + QGRDM +I+ Y +++E
Sbjct: 528 RWD--TSLLHEIPECMQTCFKVWFKLMEEVNNDVVKVQGRDMLAHIRKPWELYFNCYVQE 585
Query: 789 NVWNERGEIPTLDEYLEINHRSXXXXXXXXXXXXXXASCVDEKFCQRFLRACKLGDIMG- 613
W E G IPT +EYL+ S V + ++ + +++
Sbjct: 586 REWLEAGYIPTFEEYLKTYAISVGLGPCTLQPILLMGELVKDDVVEKVHYPSNMFELVSL 645
Query: 612 --RLVDDGRELLENDTSETTVSVITCYLKQNPGSSMSDALSYARDLFKNYYREINYWYLM 439
RL +D + S I CY+K NPG++ DA+ + + +E ++ Y
Sbjct: 646 SWRLTNDTKTYQAEKARGQQASGIACYMKDNPGATEEDAIKHICRVVDRALKEASFEYFK 705
Query: 438 -EHDIPMVCRSYCFNVIRSSLFFYTVVDGFSKCNDVIKDQIKK 313
+DIPM C+S+ FN+ FY +DG+ N+ IKD I+K
Sbjct: 706 PSNDIPMGCKSFIFNLRLCVQIFYKFIDGYGIANEEIKDYIRK 748
>gb|AAR13860.1| taxa-4(5),11(12)-diene synthase [Taxus canadensis]
Length = 862
Score = 122 bits (305), Expect = 4e-25
Identities = 80/283 (28%), Positives = 132/283 (46%), Gaps = 4/283 (1%)
Frame = -2
Query: 1149 FLRHRPIESYYYSMLSIFEPDYKDARIIQAKLTMFCYCMDDFSDNYASSQELQDIIEALE 970
F RHR E Y+ S + FEP+Y RI K+ DD +D +A+ EL+ E ++
Sbjct: 576 FTRHRVAEVYFSS--ATFEPEYSATRIAFTKIGCLQVLFDDMADIFATLDELKSFTEGVK 633
Query: 969 SWDYSTNGITKFRGDLEKVVLYLFKIMGEICSTASERQGRDMKDYIKALVVEYAKAHLRE 790
WD T+ + + ++ FK+M E+ + + QGRDM +I+ Y +++E
Sbjct: 634 RWD--TSLLHEIPECMQTCFKVWFKLMEEVNNDVVKVQGRDMLAHIRKPWELYFNCYVQE 691
Query: 789 NVWNERGEIPTLDEYLEINHRSXXXXXXXXXXXXXXASCVDEKFCQRFLRACKLGDIMG- 613
W E G IPT +EYL+ S V + ++ + +++
Sbjct: 692 REWLEAGYIPTFEEYLKTYAISVGLGPCTLQPILLMGELVKDDVVEKVHYPSNMFELVSL 751
Query: 612 --RLVDDGRELLENDTSETTVSVITCYLKQNPGSSMSDALSYARDLFKNYYREINYWYLM 439
RL +D + S I CY+K NPG++ DA+ + + +E ++ Y
Sbjct: 752 SWRLTNDTKTYQAEKARGQQASGIACYMKDNPGATEEDAIKHICRVVDRALKEASFEYFK 811
Query: 438 -EHDIPMVCRSYCFNVIRSSLFFYTVVDGFSKCNDVIKDQIKK 313
+DIPM C+S+ FN+ FY +DG+ N+ IKD I+K
Sbjct: 812 PSNDIPMGCKSFIFNLRLCVQIFYKFIDGYGIANEEIKDYIRK 854
>gb|AAR02861.1| taxadiene synthase [Taxus baccata]
Length = 862
Score = 122 bits (305), Expect = 4e-25
Identities = 80/283 (28%), Positives = 132/283 (46%), Gaps = 4/283 (1%)
Frame = -2
Query: 1149 FLRHRPIESYYYSMLSIFEPDYKDARIIQAKLTMFCYCMDDFSDNYASSQELQDIIEALE 970
F RHR E Y+ S + FEP+Y RI K+ DD +D +A+ EL+ E ++
Sbjct: 576 FTRHRVAEVYFSS--ATFEPEYSATRIAFTKIGCLQVLFDDMADIFATLDELKSFTEGVK 633
Query: 969 SWDYSTNGITKFRGDLEKVVLYLFKIMGEICSTASERQGRDMKDYIKALVVEYAKAHLRE 790
WD T+ + + ++ FK+M E+ + + QGRDM +I+ Y +++E
Sbjct: 634 RWD--TSLLHEIPECMQTCFKVWFKLMEEVNNDVVKVQGRDMLAHIRKPWELYFNCYVQE 691
Query: 789 NVWNERGEIPTLDEYLEINHRSXXXXXXXXXXXXXXASCVDEKFCQRFLRACKLGDIMG- 613
W E G IPT +EYL+ S V + ++ + +++
Sbjct: 692 REWLEAGYIPTFEEYLKTYAISVGLGPCTLQPILLMGELVKDDVVEKVHYPSNMFELVSL 751
Query: 612 --RLVDDGRELLENDTSETTVSVITCYLKQNPGSSMSDALSYARDLFKNYYREINYWYLM 439
RL +D + S I CY+K NPG++ DA+ + + +E ++ Y
Sbjct: 752 SWRLTNDTKTYQAEKARGQQASGIACYMKDNPGATEEDAIKHICRVVDRALKEASFEYFK 811
Query: 438 -EHDIPMVCRSYCFNVIRSSLFFYTVVDGFSKCNDVIKDQIKK 313
+DIPM C+S+ FN+ FY +DG+ N+ IKD I+K
Sbjct: 812 PSNDIPMGCKSFIFNLRLCVQIFYKFIDGYGIANEEIKDYIRK 854