BLASTX nr result

ID: Ephedra28_contig00000225 seq

BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Ephedra28_contig00000225
         (2027 letters)

Database: ./nr 
           37,332,560 sequences; 13,225,080,153 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AAA92669.1| 4-coumarate-CoA ligase enzyme [Pinus taeda] gi|11...   668   0.0  
gb|ACO40513.1| 4-coumarate:CoA ligase [Pinus massoniana]              668   0.0  
gb|AFJ73430.1| 4-coumarate: coenzyme A ligase, partial [Metasequ...   666   0.0  
sp|P41636.1|4CL_PINTA RecName: Full=4-coumarate--CoA ligase; Sho...   665   0.0  
gb|ACF35279.1| 4-coumarate-CoA ligase [Pinus radiata]                 664   0.0  
gb|ABR17998.1| unknown [Picea sitchensis]                             662   0.0  
gb|AFJ73433.1| 4-coumarate: coenzyme A ligase, partial [Cryptome...   653   0.0  
gb|AFJ73459.1| 4-coumarate: coenzyme A ligase, partial [Pseudota...   631   e-178
gb|AGW24660.1| 4-coumarate:coenzyme A ligase [Galtonia saundersiae]   628   e-177
ref|XP_006474168.1| PREDICTED: 4-coumarate--CoA ligase 2-like [C...   626   e-176
ref|XP_006453393.1| hypothetical protein CICLE_v10007733mg [Citr...   626   e-176
gb|AGX45528.1| 4-coumarate:CoA ligase [Pinus taeda]                   625   e-176
ref|XP_004309949.1| PREDICTED: 4-coumarate--CoA ligase 1-like [F...   624   e-176
gb|AAF91309.1|AF239686_1 4-coumarate:coA ligase 2 [Rubus idaeus]      620   e-175
gb|AFX98059.1| 4-coumarate:CoA ligase [Cunninghamia lanceolata]       620   e-175
gb|AFC89541.1| 4-coumarate: coenzyme A ligase 5 [Populus tomentosa]   620   e-175
emb|CAN69130.1| hypothetical protein VITISV_039323 [Vitis vinifera]   620   e-175
ref|XP_002272782.1| PREDICTED: 4-coumarate--CoA ligase 1 isoform...   619   e-174
gb|EXB80937.1| 4-coumarate--CoA ligase 1 [Morus notabilis]            618   e-174
gb|AAZ79469.1| 4-coumarate:coenzyme A ligase [Eucalyptus camaldu...   617   e-174

>gb|AAA92669.1| 4-coumarate-CoA ligase enzyme [Pinus taeda] gi|1143308|gb|AAB42382.1|
            4-coumarate:CoA ligase [Pinus taeda]
            gi|1143310|gb|AAB42383.1| 4-coumarate:CoA ligase [Pinus
            taeda]
          Length = 537

 Score =  668 bits (1723), Expect = 0.0
 Identities = 341/497 (68%), Positives = 402/497 (80%), Gaps = 1/497 (0%)
 Frame = +3

Query: 3    PCLIDGATERVWTYAEVELNSRKVAAGLTASGVKSGDVVMLLVQNCAEFAFTFLGASMIG 182
            PCLIDGAT+R + ++EVEL SRKVAAGL   G++ G VVMLL+ NC EFAF F+GAS+ G
Sbjct: 42   PCLIDGATDRTYCFSEVELISRKVAAGLAKLGLQQGQVVMLLLPNCIEFAFVFMGASVRG 101

Query: 183  AIVTTANPFYTPGEIAKQAAGSNARIVVTHAAFTAKLQGLDNISA-IYTIDGPVTEGCKA 359
            AIVTTANPFY PGEIAKQA  + ARI+VT AA+  KL  L +    + TID    EGC+ 
Sbjct: 102  AIVTTANPFYKPGEIAKQAKAAGARIIVTLAAYVEKLADLQSHDVLVITIDDAPKEGCQH 161

Query: 360  FSELLDADEEKDFPKGIKIDPSDVVALPYSSGTTGLPKGVKLSHRGLVASIAQLADGENP 539
             S L +ADE +     +KI P DVVALPYSSGTTGLPKGV L+H+GLV+S+AQ  DGENP
Sbjct: 162  ISVLTEADETQC--PAVKIHPDDVVALPYSSGTTGLPKGVMLTHKGLVSSVAQQVDGENP 219

Query: 540  NLYFNSEDVLLCVLPLFHIYCLHTVLLCALRVGSAMVIMPKFNITSMLQYIDKYKXXXXX 719
            NLYF+S+DV+LCVLPLFHIY L++VLLCALR G+A +IM KFN+T+ L+ I KYK     
Sbjct: 220  NLYFHSDDVILCVLPLFHIYSLNSVLLCALRAGAATLIMQKFNLTTCLELIQKYKVTVAP 279

Query: 720  XXXXXXXXXTKCDQIVAEKVSSVRMIICGGAPLAKEMEAELRERFPRVVFGQGYGMTEAG 899
                     TK   +    VSSVR+I+ G APL KE+E  LRERFP+ +FGQGYGMTEAG
Sbjct: 280  IVPPIVLDITKSPIVSQYDVSSVRIIMSGAAPLGKELEDALRERFPKAIFGQGYGMTEAG 339

Query: 900  PVLAMNLAFAKHPFPVKSGSCGTVVRNAQVKIIDTETGVSLPHNKAGEICIRGPEIMIGY 1079
            PVLAMNLAFAK+PFPVKSGSCGTVVRNAQ+KI+DTETG SLPHN+AGEICIRGPEIM GY
Sbjct: 340  PVLAMNLAFAKNPFPVKSGSCGTVVRNAQIKILDTETGESLPHNQAGEICIRGPEIMKGY 399

Query: 1080 LNDDEATEETIDKEGWLHTGDVGYIDDNEELFIVDRVKEIIKYKGFQVAPAEIEALLMQH 1259
            +ND E+T  TID+EGWLHTGDVGYIDD+EE+FIVDRVKEIIKYKGFQVAPAE+EALL+ H
Sbjct: 400  INDPESTAATIDEEGWLHTGDVGYIDDDEEIFIVDRVKEIIKYKGFQVAPAELEALLVAH 459

Query: 1260 PSISKAAVVPEKHELAGEVPVAFVVRSDGNEISEQEIKAYLEKKVIFYKRIHRVIFTDSI 1439
            PSI+ AAVVP+KHE AGEVPVAFVV+S  +EISEQEIK ++ K+VIFYK+IHRV F D+I
Sbjct: 460  PSIADAAVVPQKHEEAGEVPVAFVVKS--SEISEQEIKEFVAKQVIFYKKIHRVYFVDAI 517

Query: 1440 PIAPSGKILRKDLKARL 1490
            P +PSGKILRKDL++RL
Sbjct: 518  PKSPSGKILRKDLRSRL 534


>gb|ACO40513.1| 4-coumarate:CoA ligase [Pinus massoniana]
          Length = 537

 Score =  668 bits (1723), Expect = 0.0
 Identities = 340/497 (68%), Positives = 402/497 (80%), Gaps = 1/497 (0%)
 Frame = +3

Query: 3    PCLIDGATERVWTYAEVELNSRKVAAGLTASGVKSGDVVMLLVQNCAEFAFTFLGASMIG 182
            PCLIDGAT+R ++++EVEL SRKVAAGL   G++ G VVMLL+ NC EFAF F+GAS+ G
Sbjct: 42   PCLIDGATDRTYSFSEVELISRKVAAGLAKLGLQQGQVVMLLLPNCIEFAFVFMGASVRG 101

Query: 183  AIVTTANPFYTPGEIAKQAAGSNARIVVTHAAFTAKLQGLDNISA-IYTIDGPVTEGCKA 359
            AIVTTANPFY PGEIAKQA  + ARI+VT AA+  KL  L +    + TID    EGC+ 
Sbjct: 102  AIVTTANPFYKPGEIAKQAKAAGARIIVTLAAYVEKLADLQSHDVLVITIDDAPKEGCQH 161

Query: 360  FSELLDADEEKDFPKGIKIDPSDVVALPYSSGTTGLPKGVKLSHRGLVASIAQLADGENP 539
             S L +ADE +     +KI P DVVALPYSSGTTGLPKGV L+H+GLV+S+AQ  DGENP
Sbjct: 162  ISVLTEADETQC--PAVKIHPDDVVALPYSSGTTGLPKGVMLTHKGLVSSVAQQVDGENP 219

Query: 540  NLYFNSEDVLLCVLPLFHIYCLHTVLLCALRVGSAMVIMPKFNITSMLQYIDKYKXXXXX 719
            NLYF+SEDV+LCVLPLFHIY L++VLLCALR G+A +IM KFN+T+ L+ I KYK     
Sbjct: 220  NLYFHSEDVILCVLPLFHIYSLNSVLLCALRAGAATLIMQKFNLTTCLELIQKYKVTVAP 279

Query: 720  XXXXXXXXXTKCDQIVAEKVSSVRMIICGGAPLAKEMEAELRERFPRVVFGQGYGMTEAG 899
                     TK   +    VSSVR+I+ G APL KE+E  LRERFP+ +FGQGYGMTEAG
Sbjct: 280  IVPPIVLDITKSPIVSQYDVSSVRIIMSGAAPLGKELEDALRERFPKAIFGQGYGMTEAG 339

Query: 900  PVLAMNLAFAKHPFPVKSGSCGTVVRNAQVKIIDTETGVSLPHNKAGEICIRGPEIMIGY 1079
            PVLAMNLAFAK+PFPVKSGSCGTVVRNAQ+KI+DTETG SLPHN+AGEICIRGPEIM GY
Sbjct: 340  PVLAMNLAFAKNPFPVKSGSCGTVVRNAQIKILDTETGESLPHNQAGEICIRGPEIMKGY 399

Query: 1080 LNDDEATEETIDKEGWLHTGDVGYIDDNEELFIVDRVKEIIKYKGFQVAPAEIEALLMQH 1259
            +ND E+T  TID+EGWLHTGDVGYIDD+EE+FIVDRVKEIIKYKGFQVAPAE+EALL+ H
Sbjct: 400  INDPESTAATIDEEGWLHTGDVGYIDDDEEIFIVDRVKEIIKYKGFQVAPAELEALLVAH 459

Query: 1260 PSISKAAVVPEKHELAGEVPVAFVVRSDGNEISEQEIKAYLEKKVIFYKRIHRVIFTDSI 1439
            PSI+ AAVVP+KHE  GEVPVAFVV+S  +EISEQEIK ++ K+V+FYK+IHRV F D+I
Sbjct: 460  PSIADAAVVPQKHEETGEVPVAFVVKS--SEISEQEIKEFVAKQVVFYKKIHRVYFVDAI 517

Query: 1440 PIAPSGKILRKDLKARL 1490
            P +PSGKILRKDL++RL
Sbjct: 518  PKSPSGKILRKDLRSRL 534


>gb|AFJ73430.1| 4-coumarate: coenzyme A ligase, partial [Metasequoia
            glyptostroboides]
          Length = 494

 Score =  666 bits (1718), Expect = 0.0
 Identities = 340/497 (68%), Positives = 395/497 (79%), Gaps = 3/497 (0%)
 Frame = +3

Query: 6    CLIDGATERVWTYAEVELNSRKVAAGLTASGVKSGDVVMLLVQNCAEFAFTFLGASMIGA 185
            C+IDGAT +V++YAEVEL SRK AAGL   G+K G+VVMLL+QNC EFAF FLGASM GA
Sbjct: 1    CIIDGATGKVYSYAEVELISRKAAAGLAKVGLKQGEVVMLLLQNCVEFAFVFLGASMRGA 60

Query: 186  IVTTANPFYTPGEIAKQAAGSNARIVVTHAAFTAKL---QGLDNISAIYTIDGPVTEGCK 356
            + TTANPFYTPGEIAKQ   S ARI+VT AA+ +KL    G D    + TID P  EGC 
Sbjct: 61   VATTANPFYTPGEIAKQVKASAARIIVTQAAYVSKLGDLSGEDCDFTVITIDSP-PEGCL 119

Query: 357  AFSELLDADEEKDFPKGIKIDPSDVVALPYSSGTTGLPKGVKLSHRGLVASIAQLADGEN 536
              + L +ADE +     + I P DVVALPYSSGTTGLPKGV L+H+GLV+S+AQ  DG+N
Sbjct: 120  PITVLTEADETEC--PSVNIHPDDVVALPYSSGTTGLPKGVMLTHKGLVSSVAQQVDGDN 177

Query: 537  PNLYFNSEDVLLCVLPLFHIYCLHTVLLCALRVGSAMVIMPKFNITSMLQYIDKYKXXXX 716
            PNLYF+SEDV+LCVLPLFHIY L++VLLCALRVG+A++IMPKFN  ++L+ I KYK    
Sbjct: 178  PNLYFHSEDVILCVLPLFHIYSLNSVLLCALRVGAAILIMPKFNTVALLELIQKYKITVA 237

Query: 717  XXXXXXXXXXTKCDQIVAEKVSSVRMIICGGAPLAKEMEAELRERFPRVVFGQGYGMTEA 896
                      TK   +    +SS+R+I+ G APL KE+E  LR RFP   FGQGYGMTEA
Sbjct: 238  PIVPPIVLEITKNPIVANYDISSIRLIMSGAAPLGKELEDALRVRFPAAKFGQGYGMTEA 297

Query: 897  GPVLAMNLAFAKHPFPVKSGSCGTVVRNAQVKIIDTETGVSLPHNKAGEICIRGPEIMIG 1076
            GPVLAMNLAFAK PFPVKSGSCGTVVRNAQ+KIIDTETGVSLPHNK GEICIRGPEIM G
Sbjct: 298  GPVLAMNLAFAKEPFPVKSGSCGTVVRNAQIKIIDTETGVSLPHNKPGEICIRGPEIMKG 357

Query: 1077 YLNDDEATEETIDKEGWLHTGDVGYIDDNEELFIVDRVKEIIKYKGFQVAPAEIEALLMQ 1256
            YLND EAT  TIDKEGWLHTGDVG IDD+EE+FIVDRVKE+IKYKGFQVAPAE+EA+L+ 
Sbjct: 358  YLNDPEATARTIDKEGWLHTGDVGLIDDDEEIFIVDRVKELIKYKGFQVAPAELEAILVN 417

Query: 1257 HPSISKAAVVPEKHELAGEVPVAFVVRSDGNEISEQEIKAYLEKKVIFYKRIHRVIFTDS 1436
            HPSI  AAVVP+K+E AGEVPVAFVV+S G +ISEQEIK ++ K+V+FYKR H V F DS
Sbjct: 418  HPSIGDAAVVPQKNEAAGEVPVAFVVKSGGADISEQEIKDFVAKQVVFYKRXHTVYFVDS 477

Query: 1437 IPIAPSGKILRKDLKAR 1487
            IP +PSGKILRKDL+A+
Sbjct: 478  IPKSPSGKILRKDLRAK 494


>sp|P41636.1|4CL_PINTA RecName: Full=4-coumarate--CoA ligase; Short=4CL; AltName:
            Full=4-coumaroyl-CoA synthase gi|515501|gb|AAA92668.1|
            4-coumarate-CoA ligase enzyme [Pinus taeda]
          Length = 537

 Score =  665 bits (1715), Expect = 0.0
 Identities = 340/497 (68%), Positives = 401/497 (80%), Gaps = 1/497 (0%)
 Frame = +3

Query: 3    PCLIDGATERVWTYAEVELNSRKVAAGLTASGVKSGDVVMLLVQNCAEFAFTFLGASMIG 182
            PCLIDGAT+R + ++EVEL SRKVAAGL   G++ G VVMLL+ NC EFAF F+GAS+ G
Sbjct: 42   PCLIDGATDRTYCFSEVELISRKVAAGLAKLGLQQGQVVMLLLPNCIEFAFVFMGASVRG 101

Query: 183  AIVTTANPFYTPGEIAKQAAGSNARIVVTHAAFTAKLQGLDNISA-IYTIDGPVTEGCKA 359
            AIVTTANPFY PGEIAKQA  + ARI+VT AA+  KL  L +    + TID    EGC+ 
Sbjct: 102  AIVTTANPFYKPGEIAKQAKAAGARIIVTLAAYVEKLADLQSHDVLVITIDDAPKEGCQH 161

Query: 360  FSELLDADEEKDFPKGIKIDPSDVVALPYSSGTTGLPKGVKLSHRGLVASIAQLADGENP 539
             S L +ADE +     +KI P DVVALPYSSGTTGLPKGV L+H+GLV+S+AQ  DGENP
Sbjct: 162  ISVLTEADETQC--PAVKIHPDDVVALPYSSGTTGLPKGVMLTHKGLVSSVAQQVDGENP 219

Query: 540  NLYFNSEDVLLCVLPLFHIYCLHTVLLCALRVGSAMVIMPKFNITSMLQYIDKYKXXXXX 719
            NLYF+S+DV+LCVLPLFHIY L++VLLCALR G+A +IM KFN+T+ L+ I KYK     
Sbjct: 220  NLYFHSDDVILCVLPLFHIYSLNSVLLCALRAGAATLIMQKFNLTTCLELIQKYKVTVAP 279

Query: 720  XXXXXXXXXTKCDQIVAEKVSSVRMIICGGAPLAKEMEAELRERFPRVVFGQGYGMTEAG 899
                     TK   +    VSSVR+I+ G APL KE+E  LRERFP+ +FGQGYGMTEAG
Sbjct: 280  IVPPIVLDITKSPIVSQYDVSSVRIIMSGAAPLGKELEDALRERFPKAIFGQGYGMTEAG 339

Query: 900  PVLAMNLAFAKHPFPVKSGSCGTVVRNAQVKIIDTETGVSLPHNKAGEICIRGPEIMIGY 1079
            PVLAMNLAFAK+PFPVKSGSCGTVVRNAQ+KI+DTETG SLPHN+AGEICIRGPEIM GY
Sbjct: 340  PVLAMNLAFAKNPFPVKSGSCGTVVRNAQIKILDTETGESLPHNQAGEICIRGPEIMKGY 399

Query: 1080 LNDDEATEETIDKEGWLHTGDVGYIDDNEELFIVDRVKEIIKYKGFQVAPAEIEALLMQH 1259
            +ND E+T  TID+EGWLHTGDV YIDD+EE+FIVDRVKEIIKYKGFQVAPAE+EALL+ H
Sbjct: 400  INDPESTAATIDEEGWLHTGDVEYIDDDEEIFIVDRVKEIIKYKGFQVAPAELEALLVAH 459

Query: 1260 PSISKAAVVPEKHELAGEVPVAFVVRSDGNEISEQEIKAYLEKKVIFYKRIHRVIFTDSI 1439
            PSI+ AAVVP+KHE AGEVPVAFVV+S  +EISEQEIK ++ K+VIFYK+IHRV F D+I
Sbjct: 460  PSIADAAVVPQKHEEAGEVPVAFVVKS--SEISEQEIKEFVAKQVIFYKKIHRVYFVDAI 517

Query: 1440 PIAPSGKILRKDLKARL 1490
            P +PSGKILRKDL++RL
Sbjct: 518  PKSPSGKILRKDLRSRL 534


>gb|ACF35279.1| 4-coumarate-CoA ligase [Pinus radiata]
          Length = 537

 Score =  664 bits (1712), Expect = 0.0
 Identities = 339/497 (68%), Positives = 401/497 (80%), Gaps = 1/497 (0%)
 Frame = +3

Query: 3    PCLIDGATERVWTYAEVELNSRKVAAGLTASGVKSGDVVMLLVQNCAEFAFTFLGASMIG 182
            PCLIDGAT+R + ++EVEL SRKVAAGL   G++ G VVMLL+ NC EFAF F+GAS+ G
Sbjct: 42   PCLIDGATDRTYCFSEVELISRKVAAGLAKLGLQQGQVVMLLLPNCIEFAFVFMGASVRG 101

Query: 183  AIVTTANPFYTPGEIAKQAAGSNARIVVTHAAFTAKLQGLDNISA-IYTIDGPVTEGCKA 359
            AIVTTANPFY PGEIAKQA  + ARI+VT AA+  KL  L +    + TID    EGC+ 
Sbjct: 102  AIVTTANPFYKPGEIAKQAKAAGARIIVTLAAYVEKLADLQSHDVLVITIDDAPKEGCQH 161

Query: 360  FSELLDADEEKDFPKGIKIDPSDVVALPYSSGTTGLPKGVKLSHRGLVASIAQLADGENP 539
             S L +ADE +     + I P DVVALPYSSGTTGLPKGV L+H+GLV+S+AQ  DGENP
Sbjct: 162  ISVLTEADETQC--PAVTIHPDDVVALPYSSGTTGLPKGVMLTHKGLVSSVAQQVDGENP 219

Query: 540  NLYFNSEDVLLCVLPLFHIYCLHTVLLCALRVGSAMVIMPKFNITSMLQYIDKYKXXXXX 719
            NLYF+S+DV+LCVLPLFHIY L++VLLCALR G+A +IM KFN+T+ L+ I KYK     
Sbjct: 220  NLYFHSDDVILCVLPLFHIYSLNSVLLCALRAGAATLIMQKFNLTTCLELIQKYKVTVAP 279

Query: 720  XXXXXXXXXTKCDQIVAEKVSSVRMIICGGAPLAKEMEAELRERFPRVVFGQGYGMTEAG 899
                     TK   +    VSSVR+I+ G APL KE+E  LRERFP+ +FGQGYGMTEAG
Sbjct: 280  IVPPIVLDITKSPIVSQYDVSSVRIIMSGAAPLGKELEDALRERFPKAIFGQGYGMTEAG 339

Query: 900  PVLAMNLAFAKHPFPVKSGSCGTVVRNAQVKIIDTETGVSLPHNKAGEICIRGPEIMIGY 1079
            PVLAMNLAFAK+PFPVKSGSCGTVVRNAQ+KI+DTETG SLPH++AGEICIRGPEIM GY
Sbjct: 340  PVLAMNLAFAKNPFPVKSGSCGTVVRNAQIKILDTETGESLPHHQAGEICIRGPEIMKGY 399

Query: 1080 LNDDEATEETIDKEGWLHTGDVGYIDDNEELFIVDRVKEIIKYKGFQVAPAEIEALLMQH 1259
            +ND E+T  TID+EGWLHTGDVGYIDD+EE+FIVDRVKEIIKYKGFQVAPAE+EALL+ H
Sbjct: 400  INDPESTAATIDEEGWLHTGDVGYIDDDEEIFIVDRVKEIIKYKGFQVAPAELEALLVAH 459

Query: 1260 PSISKAAVVPEKHELAGEVPVAFVVRSDGNEISEQEIKAYLEKKVIFYKRIHRVIFTDSI 1439
            PSI+ AAVVP+KHE AGEVPVAFVV+S  +EISEQEIK ++ K+VIFYK+IHRV F D+I
Sbjct: 460  PSIADAAVVPQKHEEAGEVPVAFVVKS--SEISEQEIKEFVAKQVIFYKKIHRVYFVDAI 517

Query: 1440 PIAPSGKILRKDLKARL 1490
            P +PSGKILRKDL++RL
Sbjct: 518  PKSPSGKILRKDLRSRL 534


>gb|ABR17998.1| unknown [Picea sitchensis]
          Length = 537

 Score =  662 bits (1707), Expect = 0.0
 Identities = 337/497 (67%), Positives = 398/497 (80%), Gaps = 1/497 (0%)
 Frame = +3

Query: 3    PCLIDGATERVWTYAEVELNSRKVAAGLTASGVKSGDVVMLLVQNCAEFAFTFLGASMIG 182
            PCLIDGAT++ + +AEVEL SRKVAAGL   G++ G VVMLL+QNC EFAF F+GAS+ G
Sbjct: 42   PCLIDGATDKTYCFAEVELISRKVAAGLANLGLQQGQVVMLLLQNCIEFAFVFMGASVRG 101

Query: 183  AIVTTANPFYTPGEIAKQAAGSNARIVVTHAAFTAKLQGLDNISAIY-TIDGPVTEGCKA 359
            AIVTTANPFY P EIAKQA  + ARI+VT +A+  KL  L +   I  TIDG   EGCK 
Sbjct: 102  AIVTTANPFYKPAEIAKQAKAAGARIIVTQSAYVDKLTDLQSDDLIVITIDGAPKEGCKH 161

Query: 360  FSELLDADEEKDFPKGIKIDPSDVVALPYSSGTTGLPKGVKLSHRGLVASIAQLADGENP 539
             S L +ADE +     ++I P DVVALPYSSGTTGLPKGV L+H+GLV+S+AQ  DGENP
Sbjct: 162  ISVLTEADETQC--PSVEIHPDDVVALPYSSGTTGLPKGVMLTHKGLVSSVAQQVDGENP 219

Query: 540  NLYFNSEDVLLCVLPLFHIYCLHTVLLCALRVGSAMVIMPKFNITSMLQYIDKYKXXXXX 719
            NLYF+SEDV+LCVLPLFHIY L++VLLCALR G+A +IM KFN+T+ L+ I +YK     
Sbjct: 220  NLYFHSEDVILCVLPLFHIYSLNSVLLCALRAGAATLIMQKFNLTTCLELIQRYKVTVAP 279

Query: 720  XXXXXXXXXTKCDQIVAEKVSSVRMIICGGAPLAKEMEAELRERFPRVVFGQGYGMTEAG 899
                     TK        VSSVR+I+ G APL KE+E  LRERFP+ +FGQGYGMTEAG
Sbjct: 280  IVPPIVLDITKSPNFSQYDVSSVRIIMSGAAPLGKELEDALRERFPKAIFGQGYGMTEAG 339

Query: 900  PVLAMNLAFAKHPFPVKSGSCGTVVRNAQVKIIDTETGVSLPHNKAGEICIRGPEIMIGY 1079
            PVLAMNLAFAK  FPVKSGSCGTVVRNAQ+KI+DTETG SLPHN+AGEICIRGPEIM GY
Sbjct: 340  PVLAMNLAFAKEAFPVKSGSCGTVVRNAQIKILDTETGQSLPHNQAGEICIRGPEIMKGY 399

Query: 1080 LNDDEATEETIDKEGWLHTGDVGYIDDNEELFIVDRVKEIIKYKGFQVAPAEIEALLMQH 1259
            LND EAT  TID+EGWLHTGDVG+IDD+EE+FIVDRVKE+IKYKGFQVAPAE+EALL+ H
Sbjct: 400  LNDPEATASTIDEEGWLHTGDVGFIDDDEEIFIVDRVKELIKYKGFQVAPAELEALLVAH 459

Query: 1260 PSISKAAVVPEKHELAGEVPVAFVVRSDGNEISEQEIKAYLEKKVIFYKRIHRVIFTDSI 1439
            PSI+ AAV+P+KHE AGEVPVAFVV+   +EISEQEIK ++ K+V+FYK+IH V F D+I
Sbjct: 460  PSIADAAVIPQKHEEAGEVPVAFVVKK--SEISEQEIKDFVSKQVVFYKKIHTVYFVDAI 517

Query: 1440 PIAPSGKILRKDLKARL 1490
            P +PSGKILRKDL++RL
Sbjct: 518  PKSPSGKILRKDLRSRL 534


>gb|AFJ73433.1| 4-coumarate: coenzyme A ligase, partial [Cryptomeria japonica]
          Length = 494

 Score =  653 bits (1684), Expect = 0.0
 Identities = 336/497 (67%), Positives = 393/497 (79%), Gaps = 3/497 (0%)
 Frame = +3

Query: 6    CLIDGATERVWTYAEVELNSRKVAAGLTASGVKSGDVVMLLVQNCAEFAFTFLGASMIGA 185
            C+IDG+T +V++YAEV+L SRK AAGLT  G+  G+VVMLL+ NC EFAF FLGAS+ G 
Sbjct: 1    CIIDGSTGKVYSYAEVDLISRKAAAGLTKLGLGKGEVVMLLLPNCVEFAFVFLGASINGG 60

Query: 186  IVTTANPFYTPGEIAKQAAGSNARIVVTHAAFTAKLQGLDNISA---IYTIDGPVTEGCK 356
            + TTANPFYTPGEIAKQ   S A+IVVT AA+  KL  L N      + TID P  EGC 
Sbjct: 61   VATTANPFYTPGEIAKQVKASAAKIVVTMAAYVEKLGNLRNEECDFTVITIDAP-PEGCL 119

Query: 357  AFSELLDADEEKDFPKGIKIDPSDVVALPYSSGTTGLPKGVKLSHRGLVASIAQLADGEN 536
              S L +ADE       ++I P DVVALPYSSGTTGLPKGV L+H+GLV+S+AQ  DGEN
Sbjct: 120  PISVLTEADESAC--PNVEIHPDDVVALPYSSGTTGLPKGVMLTHKGLVSSVAQQVDGEN 177

Query: 537  PNLYFNSEDVLLCVLPLFHIYCLHTVLLCALRVGSAMVIMPKFNITSMLQYIDKYKXXXX 716
            PNLYF+SEDV+LCVLPLFHIY L++VLLCALRVG+A++IM KFNI S+L+ I +YK    
Sbjct: 178  PNLYFHSEDVILCVLPLFHIYSLNSVLLCALRVGAAILIMQKFNIVSLLELIQRYKVTVA 237

Query: 717  XXXXXXXXXXTKCDQIVAEKVSSVRMIICGGAPLAKEMEAELRERFPRVVFGQGYGMTEA 896
                      TK   +    +SS+R+I+ GGAPL K++E  +R RFP    GQGYGMTEA
Sbjct: 238  PFAPPIVLEITKNPIVQNYDISSIRLIMSGGAPLGKDLEDAVRARFPGAKSGQGYGMTEA 297

Query: 897  GPVLAMNLAFAKHPFPVKSGSCGTVVRNAQVKIIDTETGVSLPHNKAGEICIRGPEIMIG 1076
            GPVLAMNLAFAK PFPVKSGSCGTVVRNAQ+KIIDTETGVSLP NK GEICIRGPEIM G
Sbjct: 298  GPVLAMNLAFAKEPFPVKSGSCGTVVRNAQIKIIDTETGVSLPRNKPGEICIRGPEIMKG 357

Query: 1077 YLNDDEATEETIDKEGWLHTGDVGYIDDNEELFIVDRVKEIIKYKGFQVAPAEIEALLMQ 1256
            YLND EAT  TIDK GWLHTGDVGYIDD+EE+FIVDRVKE+IKYKGFQVAPAE+EALL+ 
Sbjct: 358  YLNDPEATALTIDKAGWLHTGDVGYIDDDEEIFIVDRVKELIKYKGFQVAPAELEALLVN 417

Query: 1257 HPSISKAAVVPEKHELAGEVPVAFVVRSDGNEISEQEIKAYLEKKVIFYKRIHRVIFTDS 1436
            HPSI  AAVVP+K+E AGEVPVAFVV+S+G+EI EQEIK ++ K+VI+YKRI +V F DS
Sbjct: 418  HPSIRDAAVVPQKNEAAGEVPVAFVVKSEGSEIGEQEIKDFIAKQVIYYKRIQKVYFVDS 477

Query: 1437 IPIAPSGKILRKDLKAR 1487
            IP APSGKILRKDL+A+
Sbjct: 478  IPKAPSGKILRKDLRAK 494


>gb|AFJ73459.1| 4-coumarate: coenzyme A ligase, partial [Pseudotaxus chienii]
          Length = 513

 Score =  631 bits (1628), Expect = e-178
 Identities = 325/496 (65%), Positives = 384/496 (77%), Gaps = 4/496 (0%)
 Frame = +3

Query: 6    CLIDGATERVWTYAEVELNSRKVAAGLTASGVKSGDVVMLLVQNCAEFAFTFLGASMIGA 185
            CLIDGAT ++++Y EV+L SRK AAGL   G++ G VVML+VQNC EFAF F GAS+ GA
Sbjct: 18   CLIDGATGKIFSYGEVDLISRKTAAGLAKMGLQQGQVVMLMVQNCVEFAFVFFGASIRGA 77

Query: 186  IVTTANPFYTPGEIAKQAAGSNARIVVTHAAFTAKLQGLDNISA---IYTIDGPVT-EGC 353
            IVTT NPFYTPGEIAKQ   S A+I+VT AA+  KL  L+       + TID P     C
Sbjct: 78   IVTTVNPFYTPGEIAKQVKASGAKIIVTQAAYVDKLGDLNPELCDFRVITIDNPPGGSNC 137

Query: 354  KAFSELLDADEEKDFPKGIKIDPSDVVALPYSSGTTGLPKGVKLSHRGLVASIAQLADGE 533
               S L +ADE +     + I P DVVALPYSSGTTGLPKGV L+HRGLV+S+AQ  DGE
Sbjct: 138  DHISVLTEADESEC--PSVVIHPDDVVALPYSSGTTGLPKGVMLTHRGLVSSVAQQVDGE 195

Query: 534  NPNLYFNSEDVLLCVLPLFHIYCLHTVLLCALRVGSAMVIMPKFNITSMLQYIDKYKXXX 713
            NPNLYF+SEDV+LCVLPLFHIY L++VLLCALRVG+A++IM KFNI ++L+ I KYK   
Sbjct: 196  NPNLYFHSEDVILCVLPLFHIYSLNSVLLCALRVGAAILIMQKFNIVALLELIQKYKVTV 255

Query: 714  XXXXXXXXXXXTKCDQIVAEKVSSVRMIICGGAPLAKEMEAELRERFPRVVFGQGYGMTE 893
                       TK   +    VSS+R II G APL KE+E  LR RFP   FGQGYGMTE
Sbjct: 256  APFVPPIVLEITKNPIVANYDVSSIRFIISGAAPLGKELEDALRARFPGATFGQGYGMTE 315

Query: 894  AGPVLAMNLAFAKHPFPVKSGSCGTVVRNAQVKIIDTETGVSLPHNKAGEICIRGPEIMI 1073
            AGPVLAMNLAFAK PFPVKSGSCGTVVRNAQ+KIIDTETGVSLP NK GEICIRGPEIM 
Sbjct: 316  AGPVLAMNLAFAKEPFPVKSGSCGTVVRNAQLKIIDTETGVSLPRNKPGEICIRGPEIMK 375

Query: 1074 GYLNDDEATEETIDKEGWLHTGDVGYIDDNEELFIVDRVKEIIKYKGFQVAPAEIEALLM 1253
            GYLND +AT  TIDK GWLHTGDVGYID +EE+FIVDRVKE+IK+KGFQVAPAE+EA+L+
Sbjct: 376  GYLNDPKATAGTIDKYGWLHTGDVGYIDVDEEIFIVDRVKELIKFKGFQVAPAELEAILV 435

Query: 1254 QHPSISKAAVVPEKHELAGEVPVAFVVRSDGNEISEQEIKAYLEKKVIFYKRIHRVIFTD 1433
             HP+I  AAVV +K+E AGEVPVAF+V+ +  E++EQEIK ++ K+V+FYKR+H+V F D
Sbjct: 436  NHPAIVDAAVVSQKNEAAGEVPVAFIVKFEEAEVTEQEIKDFVAKQVVFYKRLHKVYFVD 495

Query: 1434 SIPIAPSGKILRKDLK 1481
            SIP +P+GKILRKDL+
Sbjct: 496  SIPKSPAGKILRKDLR 511


>gb|AGW24660.1| 4-coumarate:coenzyme A ligase [Galtonia saundersiae]
          Length = 545

 Score =  628 bits (1620), Expect = e-177
 Identities = 309/496 (62%), Positives = 389/496 (78%)
 Frame = +3

Query: 3    PCLIDGATERVWTYAEVELNSRKVAAGLTASGVKSGDVVMLLVQNCAEFAFTFLGASMIG 182
            PC+IDGAT+RV TYAEVEL SR+VAAGL   GV+ GDV+MLL+ N  EFAF+FLGAS +G
Sbjct: 42   PCIIDGATDRVHTYAEVELASRRVAAGLHRLGVRQGDVIMLLLPNSVEFAFSFLGASYLG 101

Query: 183  AIVTTANPFYTPGEIAKQAAGSNARIVVTHAAFTAKLQGLDNISAIYTIDGPVTEGCKAF 362
            A  TTANPFYTP EI KQA  + A +VVT +    K++GL+ + A+  IDGPV +GC  F
Sbjct: 102  ATSTTANPFYTPAEIHKQAVAAGATLVVTESCHVGKVRGLEAVRAVVVIDGPVPDGCIPF 161

Query: 363  SELLDADEEKDFPKGIKIDPSDVVALPYSSGTTGLPKGVKLSHRGLVASIAQLADGENPN 542
             E+  A +E + P+ + IDP DVVALPYSSGTTGLPKGV L+HRGLV S+AQ  DG+NPN
Sbjct: 162  GEVA-AGDEAEVPE-VVIDPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGDNPN 219

Query: 543  LYFNSEDVLLCVLPLFHIYCLHTVLLCALRVGSAMVIMPKFNITSMLQYIDKYKXXXXXX 722
            L+F  +DVLLCVLPLFHIY L++VLLC LR GSA+++M KF+   M++ +++++      
Sbjct: 220  LHFTRDDVLLCVLPLFHIYSLNSVLLCGLRAGSAILLMRKFDAVRMMELVERHRVTIAPF 279

Query: 723  XXXXXXXXTKCDQIVAEKVSSVRMIICGGAPLAKEMEAELRERFPRVVFGQGYGMTEAGP 902
                     K   +    +SS+RM++ G AP+ +E+E +LRE+ P    GQGYGMTEAGP
Sbjct: 280  VPPIVVELAKNPAVDRYDLSSIRMVMSGAAPMGRELEDKLREKMPNAKLGQGYGMTEAGP 339

Query: 903  VLAMNLAFAKHPFPVKSGSCGTVVRNAQVKIIDTETGVSLPHNKAGEICIRGPEIMIGYL 1082
            VL+M LAFAK PF VKSGSCGTVVRNA++KI+D ETG  L  N+ GEICIRG +IM GYL
Sbjct: 340  VLSMCLAFAKEPFDVKSGSCGTVVRNAELKIVDPETGFPLSRNQPGEICIRGNQIMKGYL 399

Query: 1083 NDDEATEETIDKEGWLHTGDVGYIDDNEELFIVDRVKEIIKYKGFQVAPAEIEALLMQHP 1262
            ND EATE TIDKEGWLHTGD+GY+DD++E+FIVDR+KE+IKYKGFQVAPAE+EA+L+ HP
Sbjct: 400  NDPEATERTIDKEGWLHTGDIGYVDDDDEIFIVDRLKELIKYKGFQVAPAELEAMLITHP 459

Query: 1263 SISKAAVVPEKHELAGEVPVAFVVRSDGNEISEQEIKAYLEKKVIFYKRIHRVIFTDSIP 1442
            +I+ AAVVP K ELAGE+PVAFV+RS+G+EI+E EIK Y+ K+V+FYKRIHRV F ++IP
Sbjct: 460  NIADAAVVPMKDELAGELPVAFVLRSNGSEITEDEIKQYISKQVVFYKRIHRVFFIEAIP 519

Query: 1443 IAPSGKILRKDLKARL 1490
             APSGKILRKDL+A+L
Sbjct: 520  KAPSGKILRKDLRAKL 535


>ref|XP_006474168.1| PREDICTED: 4-coumarate--CoA ligase 2-like [Citrus sinensis]
          Length = 544

 Score =  626 bits (1615), Expect = e-176
 Identities = 313/498 (62%), Positives = 385/498 (77%), Gaps = 2/498 (0%)
 Frame = +3

Query: 3    PCLIDGATERVWTYAEVELNSRKVAAGLTASGVKSGDVVMLLVQNCAEFAFTFLGASMIG 182
            PC+I+G T  V+TYA+VEL SRK AAGL+  GV+ GDV+MLL+QNC +F F FLGAS IG
Sbjct: 42   PCIINGPTGDVYTYADVELTSRKFAAGLSKLGVQKGDVIMLLLQNCPQFVFAFLGASYIG 101

Query: 183  AIVTTANPFYTPGEIAKQAAGSNARIVVTHAAFTAKLQGLDNI--SAIYTIDGPVTEGCK 356
            A  TTANPFYTP EIAKQA  S A+I++T A +  K++ L     + I TID P  E C 
Sbjct: 102  ATSTTANPFYTPAEIAKQAIASKAKIIITQAVYNDKVRDLSEHHGARIVTIDPP-PENCL 160

Query: 357  AFSELLDADEEKDFPKGIKIDPSDVVALPYSSGTTGLPKGVKLSHRGLVASIAQLADGEN 536
             FSEL+   +E + P  +KI+P DVVALPYSSGTTGLPKGV L+HRGLV S+AQ  DGEN
Sbjct: 161  HFSELITQADENEIP-AVKINPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGEN 219

Query: 537  PNLYFNSEDVLLCVLPLFHIYCLHTVLLCALRVGSAMVIMPKFNITSMLQYIDKYKXXXX 716
            PNLY + EDV+LCVLPLFHIY L++VLLCALRVG+A++IM KF I  +++ + KYK    
Sbjct: 220  PNLYLHKEDVILCVLPLFHIYSLNSVLLCALRVGAAILIMQKFEIVKLMELVQKYKVTVA 279

Query: 717  XXXXXXXXXXTKCDQIVAEKVSSVRMIICGGAPLAKEMEAELRERFPRVVFGQGYGMTEA 896
                       K   +    +SS+R ++ G AP+ KE+E  +R + P    GQGYGMTEA
Sbjct: 280  PFVPPIVLAVAKSGDVDKYDMSSIRTVMSGAAPMGKELEDAVRAKLPHAKLGQGYGMTEA 339

Query: 897  GPVLAMNLAFAKHPFPVKSGSCGTVVRNAQVKIIDTETGVSLPHNKAGEICIRGPEIMIG 1076
            GPVL+M LAFAK PF +KSG+CGTVVRNA++KI+  +TG+SLP N+AGEICIRG +IM G
Sbjct: 340  GPVLSMCLAFAKEPFEIKSGACGTVVRNAEMKIVHPDTGISLPRNQAGEICIRGAQIMKG 399

Query: 1077 YLNDDEATEETIDKEGWLHTGDVGYIDDNEELFIVDRVKEIIKYKGFQVAPAEIEALLMQ 1256
            YLND EATE TIDKEGWLHTGD+GYIDD +ELFIVDR+KE+IK+KGFQVAPAE+EA+L+ 
Sbjct: 400  YLNDREATETTIDKEGWLHTGDIGYIDDEDELFIVDRLKELIKFKGFQVAPAELEAMLIA 459

Query: 1257 HPSISKAAVVPEKHELAGEVPVAFVVRSDGNEISEQEIKAYLEKKVIFYKRIHRVIFTDS 1436
            HP+IS AAVVP K E AGEVPVAFVVRS+G++I+E EIK Y+ K+V+FYKRI+RV FTDS
Sbjct: 460  HPNISDAAVVPMKDEAAGEVPVAFVVRSNGSKITEDEIKQYISKQVVFYKRINRVFFTDS 519

Query: 1437 IPIAPSGKILRKDLKARL 1490
            IP APSGKILRKDL+ +L
Sbjct: 520  IPKAPSGKILRKDLRTKL 537


>ref|XP_006453393.1| hypothetical protein CICLE_v10007733mg [Citrus clementina]
            gi|557556619|gb|ESR66633.1| hypothetical protein
            CICLE_v10007733mg [Citrus clementina]
          Length = 635

 Score =  626 bits (1615), Expect = e-176
 Identities = 313/498 (62%), Positives = 385/498 (77%), Gaps = 2/498 (0%)
 Frame = +3

Query: 3    PCLIDGATERVWTYAEVELNSRKVAAGLTASGVKSGDVVMLLVQNCAEFAFTFLGASMIG 182
            PC+I+G T  V+TYA+VEL SRK AAGL+  GV+ GDV+MLL+QNC +F F FLGAS IG
Sbjct: 133  PCIINGPTGDVYTYADVELTSRKFAAGLSKLGVQKGDVIMLLLQNCPQFVFAFLGASYIG 192

Query: 183  AIVTTANPFYTPGEIAKQAAGSNARIVVTHAAFTAKLQGLDNI--SAIYTIDGPVTEGCK 356
            A  TTANPFYTP EIAKQA  S A+I++T A +  K++ L     + I TID P  E C 
Sbjct: 193  ATSTTANPFYTPAEIAKQAIASKAKIIITQAVYNDKVRDLSEHHGARIVTIDPP-PENCL 251

Query: 357  AFSELLDADEEKDFPKGIKIDPSDVVALPYSSGTTGLPKGVKLSHRGLVASIAQLADGEN 536
             FSEL+   +E + P  +KI+P DVVALPYSSGTTGLPKGV L+HRGLV S+AQ  DGEN
Sbjct: 252  HFSELITQADENEIP-AVKINPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGEN 310

Query: 537  PNLYFNSEDVLLCVLPLFHIYCLHTVLLCALRVGSAMVIMPKFNITSMLQYIDKYKXXXX 716
            PNLY + EDV+LCVLPLFHIY L++VLLCALRVG+A++IM KF I  +++ + KYK    
Sbjct: 311  PNLYLHKEDVILCVLPLFHIYSLNSVLLCALRVGAAILIMQKFEIVKLMELVQKYKVTVA 370

Query: 717  XXXXXXXXXXTKCDQIVAEKVSSVRMIICGGAPLAKEMEAELRERFPRVVFGQGYGMTEA 896
                       K   +    +SS+R ++ G AP+ KE+E  +R + P    GQGYGMTEA
Sbjct: 371  PFVPPIVLAVAKSGDVDKYDMSSIRTVMSGAAPMGKELEDAVRAKLPHAKLGQGYGMTEA 430

Query: 897  GPVLAMNLAFAKHPFPVKSGSCGTVVRNAQVKIIDTETGVSLPHNKAGEICIRGPEIMIG 1076
            GPVL+M LAFAK PF +KSG+CGTVVRNA++KI+  +TG+SLP N+AGEICIRG +IM G
Sbjct: 431  GPVLSMCLAFAKEPFEIKSGACGTVVRNAEMKIVHPDTGISLPRNQAGEICIRGAQIMKG 490

Query: 1077 YLNDDEATEETIDKEGWLHTGDVGYIDDNEELFIVDRVKEIIKYKGFQVAPAEIEALLMQ 1256
            YLND EATE TIDKEGWLHTGD+GYIDD +ELFIVDR+KE+IK+KGFQVAPAE+EA+L+ 
Sbjct: 491  YLNDREATETTIDKEGWLHTGDIGYIDDEDELFIVDRLKELIKFKGFQVAPAELEAMLIA 550

Query: 1257 HPSISKAAVVPEKHELAGEVPVAFVVRSDGNEISEQEIKAYLEKKVIFYKRIHRVIFTDS 1436
            HP+IS AAVVP K E AGEVPVAFVVRS+G++I+E EIK Y+ K+V+FYKRI+RV FTDS
Sbjct: 551  HPNISDAAVVPMKDEAAGEVPVAFVVRSNGSKITEDEIKQYISKQVVFYKRINRVFFTDS 610

Query: 1437 IPIAPSGKILRKDLKARL 1490
            IP APSGKILRKDL+ +L
Sbjct: 611  IPKAPSGKILRKDLRTKL 628


>gb|AGX45528.1| 4-coumarate:CoA ligase [Pinus taeda]
          Length = 575

 Score =  625 bits (1611), Expect = e-176
 Identities = 311/503 (61%), Positives = 388/503 (77%), Gaps = 2/503 (0%)
 Frame = +3

Query: 3    PCLIDGATERVWTYAEVELNSRKVAAGLTASGVKSGDVVMLLVQNCAEFAFTFLGASMIG 182
            PCLI G+  ++++Y EVEL SRKVA+GL   G K GDVVMLL+ NC EF F FLGASM G
Sbjct: 68   PCLIQGSNGKIYSYGEVELISRKVASGLAKLGFKKGDVVMLLLPNCPEFVFVFLGASMAG 127

Query: 183  AIVTTANPFYTPGEIAKQAAGSNARIVVTHAAFTAKLQGL--DNISAIYTIDGPVTEGCK 356
            AI TTANPFYTP +IAKQ   S AR++VTHAA   KL+ L  ++   + TID P  +GC+
Sbjct: 128  AIATTANPFYTPSDIAKQRGASGARLIVTHAACVKKLRDLMENHGVQVITIDNP-PKGCE 186

Query: 357  AFSELLDADEEKDFPKGIKIDPSDVVALPYSSGTTGLPKGVKLSHRGLVASIAQLADGEN 536
              S LLD DE +  P    + P D VALPYSSGTTGLPKGV L+H+GLV+S+AQL DGEN
Sbjct: 187  HISLLLDGDENEYCPADCIVQPDDTVALPYSSGTTGLPKGVMLTHKGLVSSVAQLVDGEN 246

Query: 537  PNLYFNSEDVLLCVLPLFHIYCLHTVLLCALRVGSAMVIMPKFNITSMLQYIDKYKXXXX 716
            PNLY +SEDV+LCVLPLFHIY L++VLLC+LR GSA+++M KF I S+L  + ++K    
Sbjct: 247  PNLYLHSEDVVLCVLPLFHIYSLNSVLLCSLRAGSAILLMHKFEIGSLLDLVQRFKVTVA 306

Query: 717  XXXXXXXXXXTKCDQIVAEKVSSVRMIICGGAPLAKEMEAELRERFPRVVFGQGYGMTEA 896
                       K   + +  +SS+R+++ G APL KE+E  LR R P+  FGQGYGMTEA
Sbjct: 307  PVVPPIVLAFAKNALVESYDLSSIRVVLSGAAPLGKELEDALRLRLPKATFGQGYGMTEA 366

Query: 897  GPVLAMNLAFAKHPFPVKSGSCGTVVRNAQVKIIDTETGVSLPHNKAGEICIRGPEIMIG 1076
            GPVL+M LAFAK PFP+KSGSCGTVVRNAQ+KIID +TG  LP+N+ GEICIRGP+IM G
Sbjct: 367  GPVLSMCLAFAKEPFPMKSGSCGTVVRNAQMKIIDPDTGTCLPYNQPGEICIRGPQIMKG 426

Query: 1077 YLNDDEATEETIDKEGWLHTGDVGYIDDNEELFIVDRVKEIIKYKGFQVAPAEIEALLMQ 1256
            YLND E+T  TID++GWLHTGD+GYIDD+EE+FIVDRVKEIIKYKGFQV PAE+EA+L+ 
Sbjct: 427  YLNDAESTARTIDEDGWLHTGDIGYIDDDEEVFIVDRVKEIIKYKGFQVPPAELEAILIT 486

Query: 1257 HPSISKAAVVPEKHELAGEVPVAFVVRSDGNEISEQEIKAYLEKKVIFYKRIHRVIFTDS 1436
            HPSI+ AAVVP+K E+AGEVPVAFVVRS+G +++E EIK ++ K+V+FYK++H+V F  +
Sbjct: 487  HPSIADAAVVPQKDEVAGEVPVAFVVRSNGFDLTEDEIKQFVAKQVVFYKKLHKVYFIHA 546

Query: 1437 IPIAPSGKILRKDLKARLQQAAS 1505
            IP +PSGKILRKDL+A+L    S
Sbjct: 547  IPKSPSGKILRKDLRAKLSAPTS 569


>ref|XP_004309949.1| PREDICTED: 4-coumarate--CoA ligase 1-like [Fragaria vesca subsp.
            vesca]
          Length = 545

 Score =  624 bits (1608), Expect = e-176
 Identities = 309/500 (61%), Positives = 383/500 (76%), Gaps = 4/500 (0%)
 Frame = +3

Query: 3    PCLIDGATERVWTYAEVELNSRKVAAGLTASGVKSGDVVMLLVQNCAEFAFTFLGASMIG 182
            PCLI+G T + +TYA+VEL SR+VAAGL   G++ GDVVMLL+QNC EFAF FLGAS IG
Sbjct: 42   PCLINGNTGQTFTYADVELTSRRVAAGLDKLGIQQGDVVMLLLQNCPEFAFAFLGASYIG 101

Query: 183  AIVTTANPFYTPGEIAKQAAGSNARIVVTHAAFTAKLQGLDNISAIYTIDGPVTEGCKA- 359
            AI+TTANPFYTP E+AKQA  S A++++T  A+  K++    ++ +  +       C   
Sbjct: 102  AIITTANPFYTPAEVAKQATASKAKLIITQTAYVEKVKDFAKLNNVKVMSVDEASSCDKD 161

Query: 360  ---FSELLDADEEKDFPKGIKIDPSDVVALPYSSGTTGLPKGVKLSHRGLVASIAQLADG 530
               FSEL  ADE  D P  +KI+P DVVALPYSSGTTGLPKGV L+H+GLV S+AQ  DG
Sbjct: 162  VLHFSELTSADES-DIP-AVKINPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDG 219

Query: 531  ENPNLYFNSEDVLLCVLPLFHIYCLHTVLLCALRVGSAMVIMPKFNITSMLQYIDKYKXX 710
            ENPNLYF+ EDV+LCVLPLFHIY L++V LC LRVG+ ++IM KF I  +L+ ++K K  
Sbjct: 220  ENPNLYFHKEDVILCVLPLFHIYSLNSVFLCGLRVGATILIMQKFEINKLLELVEKEKVT 279

Query: 711  XXXXXXXXXXXXTKCDQIVAEKVSSVRMIICGGAPLAKEMEAELRERFPRVVFGQGYGMT 890
                         K   +    +SS+RM++ G AP+ KE+E  +R + P    GQGYGMT
Sbjct: 280  IAPFVPPIVLNIAKSPDLHQYDLSSIRMVMSGAAPMGKELEDTVRAKLPNAKLGQGYGMT 339

Query: 891  EAGPVLAMNLAFAKHPFPVKSGSCGTVVRNAQVKIIDTETGVSLPHNKAGEICIRGPEIM 1070
            EAGPVL+M LAFAK P+P+KSG+CGTVVRNA++KIID +T  SLP N++GEICIRG +IM
Sbjct: 340  EAGPVLSMCLAFAKEPYPIKSGACGTVVRNAEMKIIDPDTNESLPRNQSGEICIRGSQIM 399

Query: 1071 IGYLNDDEATEETIDKEGWLHTGDVGYIDDNEELFIVDRVKEIIKYKGFQVAPAEIEALL 1250
             GYLND EATE TIDKEGWLHTGD+GYIDD+EELFIVDR+KE+IKYKGFQVAPAE+EA+L
Sbjct: 400  KGYLNDPEATERTIDKEGWLHTGDIGYIDDDEELFIVDRLKELIKYKGFQVAPAELEAML 459

Query: 1251 MQHPSISKAAVVPEKHELAGEVPVAFVVRSDGNEISEQEIKAYLEKKVIFYKRIHRVIFT 1430
            + HP++S AAVVP K E AGEVP+AFVVRS+G++ISE +IK Y+ K+V+FYKRI RV FT
Sbjct: 460  ISHPNLSDAAVVPMKDEAAGEVPIAFVVRSNGSKISEDDIKQYVSKQVVFYKRISRVFFT 519

Query: 1431 DSIPIAPSGKILRKDLKARL 1490
            D IP APSGKILRKDL+ARL
Sbjct: 520  DKIPKAPSGKILRKDLRARL 539


>gb|AAF91309.1|AF239686_1 4-coumarate:coA ligase 2 [Rubus idaeus]
          Length = 544

 Score =  620 bits (1600), Expect = e-175
 Identities = 307/498 (61%), Positives = 384/498 (77%), Gaps = 2/498 (0%)
 Frame = +3

Query: 3    PCLIDGATERVWTYAEVELNSRKVAAGLTASGVKSGDVVMLLVQNCAEFAFTFLGASMIG 182
            PCLI+G T   +TYAEVEL SR+VAAGL   G++  DVVMLL+QNC EFAF FLGAS IG
Sbjct: 43   PCLINGNTGETFTYAEVELTSRRVAAGLDKLGIQQNDVVMLLLQNCPEFAFAFLGASYIG 102

Query: 183  AIVTTANPFYTPGEIAKQAAGSNARIVVTHAAFTAKLQGLDNIS--AIYTIDGPVTEGCK 356
            A+ TTANPFYTP E+AKQA  SNA++++T +A+  K++    ++   +  +D   +E   
Sbjct: 103  AMSTTANPFYTPAEVAKQAKASNAKLIITQSAYVDKVKDFAKLNDVKVMCVDETSSEDVL 162

Query: 357  AFSELLDADEEKDFPKGIKIDPSDVVALPYSSGTTGLPKGVKLSHRGLVASIAQLADGEN 536
             FSEL+ ADE +     +KI+P DVVALPYSSGTTGLPKGV L+H+GLV S+AQ  DGEN
Sbjct: 163  HFSELMSADESET--PAVKINPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGEN 220

Query: 537  PNLYFNSEDVLLCVLPLFHIYCLHTVLLCALRVGSAMVIMPKFNITSMLQYIDKYKXXXX 716
            PNLYF+ EDV+LCVLPLFHIY L++V LC LRVG+A++IM KF I  +L+ ++K K    
Sbjct: 221  PNLYFHKEDVILCVLPLFHIYSLNSVFLCGLRVGAAILIMQKFEINKLLELVEKEKVTIA 280

Query: 717  XXXXXXXXXXTKCDQIVAEKVSSVRMIICGGAPLAKEMEAELRERFPRVVFGQGYGMTEA 896
                       KC  +    +SS+RM++ G AP+ KE+E  +R + P    GQGYGMTEA
Sbjct: 281  PFVPPIVLSIAKCPDLHRYDLSSIRMVMSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEA 340

Query: 897  GPVLAMNLAFAKHPFPVKSGSCGTVVRNAQVKIIDTETGVSLPHNKAGEICIRGPEIMIG 1076
            GPVL+M LAFAK P+ +KSG+CGTVVRNA++KIID +T  SLP N++GEICIRG +IM G
Sbjct: 341  GPVLSMCLAFAKEPYEIKSGACGTVVRNAEMKIIDPDTNESLPRNQSGEICIRGSQIMKG 400

Query: 1077 YLNDDEATEETIDKEGWLHTGDVGYIDDNEELFIVDRVKEIIKYKGFQVAPAEIEALLMQ 1256
            YLND EATE TIDKEGWLHTGD+GYIDD++ELFIVDR+KE+IKYKGFQVAPAE+EA+L+ 
Sbjct: 401  YLNDPEATENTIDKEGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEAMLIS 460

Query: 1257 HPSISKAAVVPEKHELAGEVPVAFVVRSDGNEISEQEIKAYLEKKVIFYKRIHRVIFTDS 1436
            HP++S AAVV  K E AGEVPVAFVVRS+G++ISE +IK Y+ K+V+FYKRI +V FTD 
Sbjct: 461  HPNLSDAAVVSMKDEAAGEVPVAFVVRSNGSKISEDDIKQYISKQVVFYKRISKVFFTDK 520

Query: 1437 IPIAPSGKILRKDLKARL 1490
            IP APSGKILRKDL+ARL
Sbjct: 521  IPKAPSGKILRKDLRARL 538


>gb|AFX98059.1| 4-coumarate:CoA ligase [Cunninghamia lanceolata]
          Length = 565

 Score =  620 bits (1598), Expect = e-175
 Identities = 307/497 (61%), Positives = 382/497 (76%)
 Frame = +3

Query: 3    PCLIDGATERVWTYAEVELNSRKVAAGLTASGVKSGDVVMLLVQNCAEFAFTFLGASMIG 182
            PCLI+G++ +++TY +VEL SRK A+GL+  G+  GDVVMLL+ NC EFAF FLG S+ G
Sbjct: 66   PCLIEGSSGKIYTYGDVELISRKAASGLSKMGIGKGDVVMLLLHNCPEFAFVFLGTSISG 125

Query: 183  AIVTTANPFYTPGEIAKQAAGSNARIVVTHAAFTAKLQGLDNISAIYTIDGPVTEGCKAF 362
             +VTTANPFYTP +I+KQ   SNARIVVTHAA+  KL        + T+D P  EGC   
Sbjct: 126  GVVTTANPFYTPADISKQLRASNARIVVTHAAYVEKLTDFLPHLKVLTVDNP-PEGCSHI 184

Query: 363  SELLDADEEKDFPKGIKIDPSDVVALPYSSGTTGLPKGVKLSHRGLVASIAQLADGENPN 542
            SELL  DEEK     ++I P D VALPYSSGTTGLPKGV L+H+ L++S+AQ  DG NPN
Sbjct: 185  SELLQGDEEKC--PAVEIHPDDAVALPYSSGTTGLPKGVILTHKSLLSSVAQQVDGLNPN 242

Query: 543  LYFNSEDVLLCVLPLFHIYCLHTVLLCALRVGSAMVIMPKFNITSMLQYIDKYKXXXXXX 722
            LY +SEDV+LCVLPLFHIY L++VLLC+LR G+A+++M KF I ++L  I  YK      
Sbjct: 243  LYLHSEDVVLCVLPLFHIYSLNSVLLCSLRAGAAILLMHKFEIATLLHLIHTYKVTVAAV 302

Query: 723  XXXXXXXXTKCDQIVAEKVSSVRMIICGGAPLAKEMEAELRERFPRVVFGQGYGMTEAGP 902
                     K   I    ++S+R+++ G APL KE+E  L  R PR VFGQGYGMTEAGP
Sbjct: 303  VPPIVLAIAKNPMIDHHDLTSIRIVLSGAAPLGKELELALTTRIPRAVFGQGYGMTEAGP 362

Query: 903  VLAMNLAFAKHPFPVKSGSCGTVVRNAQVKIIDTETGVSLPHNKAGEICIRGPEIMIGYL 1082
            VL+M L FAK PFP KSGSCGTVVRNAQ+KIID ETG SLP+NK GEICIRGP+IM GYL
Sbjct: 363  VLSMCLGFAKEPFPTKSGSCGTVVRNAQMKIIDPETGESLPYNKPGEICIRGPQIMKGYL 422

Query: 1083 NDDEATEETIDKEGWLHTGDVGYIDDNEELFIVDRVKEIIKYKGFQVAPAEIEALLMQHP 1262
            ND EAT  TIDK+GWLHTGD+GYID+ EE+FIVDRVKE+IKYKGFQV PAE+E++L+ HP
Sbjct: 423  NDPEATARTIDKDGWLHTGDIGYIDEGEEVFIVDRVKELIKYKGFQVPPAELESILITHP 482

Query: 1263 SISKAAVVPEKHELAGEVPVAFVVRSDGNEISEQEIKAYLEKKVIFYKRIHRVIFTDSIP 1442
            SI+ AAVVP+K+E AGEVPVAFVVRS+G +++E EIK ++ K+V+FYKR+H+V F  +IP
Sbjct: 483  SIADAAVVPQKNEAAGEVPVAFVVRSNGFDLTEDEIKQFVAKQVVFYKRLHKVYFVHAIP 542

Query: 1443 IAPSGKILRKDLKARLQ 1493
             +P+GKILRKDL+A+L+
Sbjct: 543  KSPAGKILRKDLRAKLE 559


>gb|AFC89541.1| 4-coumarate: coenzyme A ligase 5 [Populus tomentosa]
          Length = 545

 Score =  620 bits (1598), Expect = e-175
 Identities = 309/500 (61%), Positives = 388/500 (77%), Gaps = 4/500 (0%)
 Frame = +3

Query: 3    PCLIDGATERVWTYAEVELNSRKVAAGLTASGVKSGDVVMLLVQNCAEFAFTFLGASMIG 182
            PCLI+G T  + TYAEVEL SRKVA+GL+  G+K GDV++LL+QN  EF F FLGAS+IG
Sbjct: 43   PCLINGPTGEIHTYAEVELTSRKVASGLSKLGIKQGDVILLLLQNSPEFVFAFLGASIIG 102

Query: 183  AIVTTANPFYTPGEIAKQAAGSNARIVVTHAAFTAKLQGL----DNISAIYTIDGPVTEG 350
            AI TTANPFYTP E+AKQA  S A++++T A +  K+Q      D++  I T+D P  E 
Sbjct: 103  AISTTANPFYTPAEVAKQATASKAKLIITQAVYAEKVQQFVKENDHVK-IVTVDSP-PEN 160

Query: 351  CKAFSELLDADEEKDFPKGIKIDPSDVVALPYSSGTTGLPKGVKLSHRGLVASIAQLADG 530
               FSEL ++DE+ D P  ++I+P DVVALPYSSGTTGLPKGV L+H+GLV S+AQ  DG
Sbjct: 161  YLHFSELTNSDED-DIP-AVEINPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDG 218

Query: 531  ENPNLYFNSEDVLLCVLPLFHIYCLHTVLLCALRVGSAMVIMPKFNITSMLQYIDKYKXX 710
            ENPNLYF+ +DV+LCVLPLFHIY L++VLLC LRVGSA+++M KF I ++++ + KYK  
Sbjct: 219  ENPNLYFHEKDVILCVLPLFHIYSLNSVLLCGLRVGSAILLMQKFEIVTLMELVQKYKVT 278

Query: 711  XXXXXXXXXXXXTKCDQIVAEKVSSVRMIICGGAPLAKEMEAELRERFPRVVFGQGYGMT 890
                         KC  +    +SS+R ++ G AP+ KE+E  +R + P    GQGYGMT
Sbjct: 279  IAPFVPPVVLAVAKCPVVDKYDLSSIRTVMSGAAPMGKELEDTVRAKLPNAKLGQGYGMT 338

Query: 891  EAGPVLAMNLAFAKHPFPVKSGSCGTVVRNAQVKIIDTETGVSLPHNKAGEICIRGPEIM 1070
            EAGPVL+M LAFA+ PF +KSG+CGTVVRNA++KI+D +TG SLP N+AGEICIRG +IM
Sbjct: 339  EAGPVLSMCLAFAREPFEIKSGACGTVVRNAEMKIVDPDTGRSLPRNQAGEICIRGSQIM 398

Query: 1071 IGYLNDDEATEETIDKEGWLHTGDVGYIDDNEELFIVDRVKEIIKYKGFQVAPAEIEALL 1250
             GYLND EATE TID +GWLHTGD+GYIDD++ELFIVDR+KE+IKYKGFQVAPAE+EA+L
Sbjct: 399  KGYLNDPEATERTIDNDGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEAML 458

Query: 1251 MQHPSISKAAVVPEKHELAGEVPVAFVVRSDGNEISEQEIKAYLEKKVIFYKRIHRVIFT 1430
            + HP IS  AVVP K E AGEVPVAFVVR++G++I+E EIK Y+ K+V+FYKRI RV FT
Sbjct: 459  IAHPDISDCAVVPMKDEAAGEVPVAFVVRANGSKITEDEIKQYISKQVVFYKRISRVFFT 518

Query: 1431 DSIPIAPSGKILRKDLKARL 1490
            +SIP APSGKILRKDL+ARL
Sbjct: 519  ESIPKAPSGKILRKDLRARL 538


>emb|CAN69130.1| hypothetical protein VITISV_039323 [Vitis vinifera]
          Length = 548

 Score =  620 bits (1598), Expect = e-175
 Identities = 310/498 (62%), Positives = 381/498 (76%), Gaps = 2/498 (0%)
 Frame = +3

Query: 3    PCLIDGATERVWTYAEVELNSRKVAAGLTASGVKSGDVVMLLVQNCAEFAFTFLGASMIG 182
            PC+IDGA   ++TYA+VEL +R+V AGL   G+K G+V+MLL+QNC EF F FLGAS IG
Sbjct: 48   PCVIDGANGDIYTYADVELTARRVGAGLHKMGIKQGEVIMLLLQNCTEFVFAFLGASYIG 107

Query: 183  AIVTTANPFYTPGEIAKQAAGSNARIVVTHAAFTAKLQGL--DNISAIYTIDGPVTEGCK 356
            A  TTANPFY P EI KQA  S  R+++T A ++ K      +N   +  ID P  EGC 
Sbjct: 108  ATSTTANPFYMPAEIEKQAKASKCRLIITQACYSGKAMSFAEENDVKVMCIDTP-PEGCL 166

Query: 357  AFSELLDADEEKDFPKGIKIDPSDVVALPYSSGTTGLPKGVKLSHRGLVASIAQLADGEN 536
             FSEL +A+E  D P  +KI+P DVVALPYSSGTTGLPKGV L+HRG V S+AQ  DGEN
Sbjct: 167  HFSELTNABEA-DLPT-VKINPDDVVALPYSSGTTGLPKGVMLTHRGQVTSVAQQVDGEN 224

Query: 537  PNLYFNSEDVLLCVLPLFHIYCLHTVLLCALRVGSAMVIMPKFNITSMLQYIDKYKXXXX 716
            PNLYF+SEDV+LCVLPLFH+Y L++VLLC LRVG+A++IM KF I  +L+ I+KYK    
Sbjct: 225  PNLYFHSEDVILCVLPLFHVYSLNSVLLCGLRVGAAILIMQKFEIIKLLELINKYKVTIA 284

Query: 717  XXXXXXXXXXTKCDQIVAEKVSSVRMIICGGAPLAKEMEAELRERFPRVVFGQGYGMTEA 896
                       K   +    +SS+R ++ G AP+ KE+E  +R + P    GQGYGMTEA
Sbjct: 285  PFVPPIVLAIAKSPVVDEYDLSSIRTVMSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEA 344

Query: 897  GPVLAMNLAFAKHPFPVKSGSCGTVVRNAQVKIIDTETGVSLPHNKAGEICIRGPEIMIG 1076
            GPVLAM LAFAK PF +KSGSCGTVVRNAQ+KI+D +TG SLP N+ GEICIRG +IM G
Sbjct: 345  GPVLAMCLAFAKEPFEIKSGSCGTVVRNAQMKILDPDTGASLPRNQPGEICIRGHQIMKG 404

Query: 1077 YLNDDEATEETIDKEGWLHTGDVGYIDDNEELFIVDRVKEIIKYKGFQVAPAEIEALLMQ 1256
            YLND EATE TIDKEGWLHTGD+GYIDD++ELFIVDR+KE+IKYKGFQVAPAE+EA+L+ 
Sbjct: 405  YLNDPEATERTIDKEGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEAMLIA 464

Query: 1257 HPSISKAAVVPEKHELAGEVPVAFVVRSDGNEISEQEIKAYLEKKVIFYKRIHRVIFTDS 1436
            HP++S AAVV  K E AGEVPVAF+VRS+G++I+E EIK Y+ K+V+FYKRI+RV F ++
Sbjct: 465  HPNVSDAAVVSMKDEAAGEVPVAFIVRSNGSKITEDEIKQYISKQVVFYKRIYRVFFVET 524

Query: 1437 IPIAPSGKILRKDLKARL 1490
            IP APSGKILRKDL+ARL
Sbjct: 525  IPKAPSGKILRKDLRARL 542


>ref|XP_002272782.1| PREDICTED: 4-coumarate--CoA ligase 1 isoform 1 [Vitis vinifera]
          Length = 548

 Score =  619 bits (1596), Expect = e-174
 Identities = 310/498 (62%), Positives = 381/498 (76%), Gaps = 2/498 (0%)
 Frame = +3

Query: 3    PCLIDGATERVWTYAEVELNSRKVAAGLTASGVKSGDVVMLLVQNCAEFAFTFLGASMIG 182
            PC+IDGA   ++TYA+VEL +R+V AGL   G+K G+V+MLL+QNC EF F FLGAS IG
Sbjct: 48   PCVIDGANGDIYTYADVELTARRVGAGLHKMGIKQGEVIMLLLQNCTEFVFAFLGASYIG 107

Query: 183  AIVTTANPFYTPGEIAKQAAGSNARIVVTHAAFTAKLQGL--DNISAIYTIDGPVTEGCK 356
            A  TTANPFY P EI KQA  S  R+++T A ++ K      +N   +  ID P  EGC 
Sbjct: 108  ATSTTANPFYMPAEIEKQAKASKCRLIITQACYSEKAMSFAEENDVKVMCIDTP-PEGCL 166

Query: 357  AFSELLDADEEKDFPKGIKIDPSDVVALPYSSGTTGLPKGVKLSHRGLVASIAQLADGEN 536
             FSEL +ADE  D P  +KI+P DVVALPYSSGTTGLPKGV L+HRG V S+AQ  DG+N
Sbjct: 167  HFSELTNADEA-DLPT-VKINPDDVVALPYSSGTTGLPKGVMLTHRGQVTSVAQQVDGDN 224

Query: 537  PNLYFNSEDVLLCVLPLFHIYCLHTVLLCALRVGSAMVIMPKFNITSMLQYIDKYKXXXX 716
            PNLYF+SEDV+LCVLPLFH+Y L++VLLC LRVG+A++IM KF I  +L+ I+KYK    
Sbjct: 225  PNLYFHSEDVILCVLPLFHVYSLNSVLLCGLRVGAAILIMQKFEIIKLLELINKYKVTIA 284

Query: 717  XXXXXXXXXXTKCDQIVAEKVSSVRMIICGGAPLAKEMEAELRERFPRVVFGQGYGMTEA 896
                       K   +    +SS+R ++ G AP+ KE+E  +R + P    GQGYGMTEA
Sbjct: 285  PFVPPIVLAIAKSPVVDEYDLSSIRTVMSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEA 344

Query: 897  GPVLAMNLAFAKHPFPVKSGSCGTVVRNAQVKIIDTETGVSLPHNKAGEICIRGPEIMIG 1076
            GPVLAM LAFAK PF +KSGSCGTVVRNAQ+KI+D +TG SLP N+ GEICIRG +IM G
Sbjct: 345  GPVLAMCLAFAKEPFEIKSGSCGTVVRNAQMKILDPDTGASLPRNQPGEICIRGHQIMKG 404

Query: 1077 YLNDDEATEETIDKEGWLHTGDVGYIDDNEELFIVDRVKEIIKYKGFQVAPAEIEALLMQ 1256
            YLND EATE TIDKEGWLHTGD+GYIDD++ELFIVDR+KE+IKYKGFQVAPAE+EA+L+ 
Sbjct: 405  YLNDPEATERTIDKEGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEAMLIA 464

Query: 1257 HPSISKAAVVPEKHELAGEVPVAFVVRSDGNEISEQEIKAYLEKKVIFYKRIHRVIFTDS 1436
            HP++S AAVV  K E AGEVPVAF+VRS+G++I+E EIK Y+ K+V+FYKRI+RV F ++
Sbjct: 465  HPNVSDAAVVSMKDEAAGEVPVAFIVRSNGSKITEDEIKQYISKQVVFYKRIYRVFFVET 524

Query: 1437 IPIAPSGKILRKDLKARL 1490
            IP APSGKILRKDL+ARL
Sbjct: 525  IPKAPSGKILRKDLRARL 542


>gb|EXB80937.1| 4-coumarate--CoA ligase 1 [Morus notabilis]
          Length = 646

 Score =  618 bits (1594), Expect = e-174
 Identities = 305/498 (61%), Positives = 385/498 (77%), Gaps = 2/498 (0%)
 Frame = +3

Query: 3    PCLIDGATERVWTYAEVELNSRKVAAGLTASGVKSGDVVMLLVQNCAEFAFTFLGASMIG 182
            PCLI+G+T    +YA+VEL +RKVAAGL   G+K GDV++LL+QNC EF FTFL AS IG
Sbjct: 49   PCLINGSTGETHSYADVELTARKVAAGLDEMGIKQGDVILLLLQNCPEFVFTFLAASYIG 108

Query: 183  AIVTTANPFYTPGEIAKQAAGSNARIVVTHAAFTAKLQGL--DNISAIYTIDGPVTEGCK 356
            AI TTAN FYTP EIAKQA  S A++++T + +  K++    +N   I  ID    EGC 
Sbjct: 109  AISTTANSFYTPAEIAKQAKASKAKLILTVSTYVDKVKNFADENSVKIVCIDDAPPEGCL 168

Query: 357  AFSELLDADEEKDFPKGIKIDPSDVVALPYSSGTTGLPKGVKLSHRGLVASIAQLADGEN 536
             FSEL +ADE       +KI+P DVVALPYSSGTTGLPKGV L+HRGLV S+AQ  DGEN
Sbjct: 169  HFSELSNADESAI--PAVKINPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGEN 226

Query: 537  PNLYFNSEDVLLCVLPLFHIYCLHTVLLCALRVGSAMVIMPKFNITSMLQYIDKYKXXXX 716
            PNLYF+SEDV+LCVLPLFHIY L+++LLC LRVG+A++IM KF I  +L+ ++KYK    
Sbjct: 227  PNLYFHSEDVILCVLPLFHIYSLNSILLCGLRVGAAILIMQKFEINKLLELVEKYKVTIA 286

Query: 717  XXXXXXXXXXTKCDQIVAEKVSSVRMIICGGAPLAKEMEAELRERFPRVVFGQGYGMTEA 896
                       K   +    +SS+RM++ GGAP+ K++E  ++++ P    GQGYGMTEA
Sbjct: 287  PFVPPIVLAIAKSPDLHRYDLSSIRMVMSGGAPMGKDLEEAVKDKLPHAKLGQGYGMTEA 346

Query: 897  GPVLAMNLAFAKHPFPVKSGSCGTVVRNAQVKIIDTETGVSLPHNKAGEICIRGPEIMIG 1076
            GPVL+M LAFAK PFP+KSG+CGTVVRNA++KIID +TG SLP N+AGEICIRG +IM G
Sbjct: 347  GPVLSMCLAFAKEPFPIKSGACGTVVRNAEMKIIDPDTGASLPRNQAGEICIRGSQIMKG 406

Query: 1077 YLNDDEATEETIDKEGWLHTGDVGYIDDNEELFIVDRVKEIIKYKGFQVAPAEIEALLMQ 1256
            Y+ND EAT+ TID+ GWLHTGD+GYIDD++ELFIVDR+KE+IKYKGFQVAPAE+EA+L+ 
Sbjct: 407  YVNDPEATKATIDERGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEAMLIA 466

Query: 1257 HPSISKAAVVPEKHELAGEVPVAFVVRSDGNEISEQEIKAYLEKKVIFYKRIHRVIFTDS 1436
            HP+IS AAVVP   E AGE+PVA+VVRS+G++I+E +IK Y+  +V+FYKRI +VIF D 
Sbjct: 467  HPNISDAAVVPMNDEAAGEIPVAYVVRSNGSKITENDIKKYISDQVVFYKRIGKVIFIDK 526

Query: 1437 IPIAPSGKILRKDLKARL 1490
            IP +PSGKILRKDL+ARL
Sbjct: 527  IPKSPSGKILRKDLRARL 544


>gb|AAZ79469.1| 4-coumarate:coenzyme A ligase [Eucalyptus camaldulensis]
          Length = 544

 Score =  617 bits (1592), Expect = e-174
 Identities = 311/498 (62%), Positives = 384/498 (77%), Gaps = 2/498 (0%)
 Frame = +3

Query: 3    PCLIDGATERVWTYAEVELNSRKVAAGLTASGVKSGDVVMLLVQNCAEFAFTFLGASMIG 182
            PC+I+GAT R +TYAEVEL SR+V+AGL   GV  GDV+MLL+QNC EF F FLGAS  G
Sbjct: 44   PCVINGATGRTYTYAEVELISRRVSAGLNGLGVGQGDVIMLLLQNCPEFVFAFLGASYRG 103

Query: 183  AIVTTANPFYTPGEIAKQAAGSNARIVVTHAAFTAKLQGL--DNISAIYTIDGPVTEGCK 356
            AI TTANPFYTPGEIAKQA+ + A+IV+T AA+  K++    +N   +  ID    EGC 
Sbjct: 104  AISTTANPFYTPGEIAKQASAAQAKIVITQAAYADKVRPFAEENGLRVVCID-TAPEGCL 162

Query: 357  AFSELLDADEEKDFPKGIKIDPSDVVALPYSSGTTGLPKGVKLSHRGLVASIAQLADGEN 536
             FSEL+ ADE       +K  P DV+ALPYSSGTTGLPKGV L+HRG V S+AQ  DG+N
Sbjct: 163  HFSELMQADENAAPAADVK--PDDVLALPYSSGTTGLPKGVMLTHRGQVTSVAQQVDGDN 220

Query: 537  PNLYFNSEDVLLCVLPLFHIYCLHTVLLCALRVGSAMVIMPKFNITSMLQYIDKYKXXXX 716
            PNLYF+ EDV+LC LPLFHIY L++V+ CALRVG+A++IM KF I ++++ + +Y+    
Sbjct: 221  PNLYFHKEDVILCTLPLFHIYSLNSVMFCALRVGAAILIMQKFEIMALMELVQRYRVTIL 280

Query: 717  XXXXXXXXXXTKCDQIVAEKVSSVRMIICGGAPLAKEMEAELRERFPRVVFGQGYGMTEA 896
                       K  ++    +SS+R I+ G AP+ KE+E  +R + P    GQGYGMTEA
Sbjct: 281  PIVPPIVLAIAKSAEVDRYDLSSIRTIMSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEA 340

Query: 897  GPVLAMNLAFAKHPFPVKSGSCGTVVRNAQVKIIDTETGVSLPHNKAGEICIRGPEIMIG 1076
            GPVLAM LAFAK PF +KSG+CGTVVRNA++KI+D ETG SLP N+AGEICIRG +IM G
Sbjct: 341  GPVLAMCLAFAKEPFEIKSGACGTVVRNAEMKIVDPETGASLPRNQAGEICIRGHQIMKG 400

Query: 1077 YLNDDEATEETIDKEGWLHTGDVGYIDDNEELFIVDRVKEIIKYKGFQVAPAEIEALLMQ 1256
            YLND EAT  TIDKEGWLHTGD+GYIDD++ELFIVDR+KE+IKYKGFQVAPAE+EA+L+ 
Sbjct: 401  YLNDPEATANTIDKEGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEAMLIA 460

Query: 1257 HPSISKAAVVPEKHELAGEVPVAFVVRSDGNEISEQEIKAYLEKKVIFYKRIHRVIFTDS 1436
            HPSIS AAVVP K E+A EVPVAFVV+S+G+ I+E EIK Y+ K+V+FYKRI+RV FTD+
Sbjct: 461  HPSISDAAVVPMKDEVASEVPVAFVVKSNGSVITEDEIKQYISKQVVFYKRINRVFFTDA 520

Query: 1437 IPIAPSGKILRKDLKARL 1490
            IP APSGKILRKDL+A+L
Sbjct: 521  IPKAPSGKILRKDLRAKL 538


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