BLASTX nr result
ID: Ephedra27_contig00027283
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Ephedra27_contig00027283
(731 letters)
Database: ./nr
37,332,560 sequences; 13,225,080,153 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|ACJ76783.1| berberine bridge enzyme 1 [Argemone mexicana] 194 3e-47
gb|ACJ76784.1| berberine bridge enzyme 2 [Argemone mexicana] 189 8e-46
dbj|BAM44344.1| berberine bridge enzyme [Coptis japonica] 187 3e-45
gb|AAU20769.1| berberine bridge enzyme [Thalictrum flavum subsp.... 181 3e-43
gb|AGL44333.1| FAD-dependent oxidoreductase, partial [Papaver so... 179 8e-43
gb|AAD17487.1| berberine bridge enzyme [Berberis stolonifera] 179 1e-42
sp|P93479.1|RETO_PAPSO RecName: Full=Reticuline oxidase; AltName... 177 2e-42
gb|ACO90226.1| putative berberine bridge enzyme [Papaver bractea... 176 7e-42
gb|EXC32491.1| Reticuline oxidase [Morus notabilis] 173 6e-41
dbj|BAK18780.1| berberine bridge enzyme-like protein [Nicotiana ... 171 2e-40
pdb|3D2D|A Chain A, Structure Of Berberine Bridge Enzyme In Comp... 171 2e-40
sp|P30986.1|RETO_ESCCA RecName: Full=Reticuline oxidase; AltName... 170 4e-40
gb|ACO90218.1| berberine bridge enzyme [Eschscholzia californica] 170 4e-40
ref|XP_006846477.1| hypothetical protein AMTR_s00018p00124850 [A... 169 7e-40
pdb|4EC3|A Chain A, Structure Of Berberine Bridge Enzyme, H174a ... 169 9e-40
pdb|3GSY|A Chain A, Structure Of Berberine Bridge Enzyme In Comp... 169 9e-40
dbj|BAK18779.1| berberine bridge enzyme-like protein [Nicotiana ... 169 1e-39
pdb|3FW9|A Chain A, Structure Of Berberine Bridge Enzyme In Comp... 169 1e-39
pdb|3FW7|A Chain A, Structure Of Berberine Bridge Enzyme, H104a ... 169 1e-39
ref|XP_004158082.1| PREDICTED: reticuline oxidase-like protein-l... 168 1e-39
>gb|ACJ76783.1| berberine bridge enzyme 1 [Argemone mexicana]
Length = 554
Score = 194 bits (492), Expect = 3e-47
Identities = 100/236 (42%), Positives = 143/236 (60%), Gaps = 2/236 (0%)
Frame = -1
Query: 707 LQSTMIMLALLNVWSFARSQDVDSLQSCLTAGGLTNVSTHADFNRYDYILKY--SLQNLR 534
L S++ ++ +S D + SCLT+ G+ N +T + + DY+ Y S+QN
Sbjct: 15 LYSSLFIIIFFFFFSVLTCALSDDILSCLTSNGVHNYTTPSSDSNSDYLRLYHLSIQNPL 74
Query: 533 YTERWVPKPALIILPMKKEEVPAAVKCCVETGWMMCIRSGGDDFEGLSSWADVPFVIVDL 354
+ + + KP+LI+LP KEE+ V+CC W + +RSGG +EGLS AD PFV++DL
Sbjct: 75 FEKSTISKPSLIVLPRNKEELSNTVRCCTRGSWTIRLRSGGHSYEGLSYTADTPFVLIDL 134
Query: 353 MNLVSITVDIDTETAWAEAGATIGQLYAAIGNATDELAFAAGDCPTVXXXXXXXXXXXGS 174
MNL I++DID+ETAW E+GAT+G+LY AI TD L F AG CPTV G
Sbjct: 135 MNLNRISIDIDSETAWVESGATLGELYYAITELTDSLGFTAGWCPTVGSGGHISGGGFGM 194
Query: 173 YHRAFGLASDSVDDVIMVDAKGDLLNKTNMTEDVYWAXXXXXXXXXXXXVAWKLKL 6
R +GLA+D+V+DVI++D+KG +L++ M EDV+WA AWK+KL
Sbjct: 195 MSRKYGLAADNVEDVILIDSKGAILDRKLMGEDVFWAVRGGGGGVWGAIYAWKIKL 250
>gb|ACJ76784.1| berberine bridge enzyme 2 [Argemone mexicana]
Length = 536
Score = 189 bits (480), Expect = 8e-46
Identities = 101/243 (41%), Positives = 145/243 (59%), Gaps = 2/243 (0%)
Frame = -1
Query: 728 TSIKLSFLQSTMIMLALLNVWSFARSQDVDSLQSCLTAGGLTNVSTHADFNRYDYILKY- 552
T I +F S I ++L A S D+ SCLT+ G+ N +T + + DY+ +
Sbjct: 9 TKITKNFYSSLFIFFSVLTC---ALSDDI---LSCLTSNGVHNYTTPSSDSNSDYLRLFH 62
Query: 551 -SLQNLRYTERWVPKPALIILPMKKEEVPAAVKCCVETGWMMCIRSGGDDFEGLSSWADV 375
S+QN + + + KP+LI+LP KEE+ V+CC W + +RSGG +EGLS AD
Sbjct: 63 LSIQNPLFKKSTISKPSLIVLPGNKEELSNTVRCCTRGSWTIRLRSGGHSYEGLSYTADT 122
Query: 374 PFVIVDLMNLVSITVDIDTETAWAEAGATIGQLYAAIGNATDELAFAAGDCPTVXXXXXX 195
PFV++DLMNL I++D+D+ETAW E+GAT+G+LY AI +TD F AG CPTV
Sbjct: 123 PFVLIDLMNLNRISIDMDSETAWVESGATVGELYYAISESTDSFGFTAGWCPTVGTGGHI 182
Query: 194 XXXXXGSYHRAFGLASDSVDDVIMVDAKGDLLNKTNMTEDVYWAXXXXXXXXXXXXVAWK 15
G R +GLA+D+V+DVI++D+ G +L++ M EDV+WA AWK
Sbjct: 183 SGGGFGMMSRKYGLAADNVEDVILIDSNGAILDRKLMGEDVFWAVRGGGGGVWGAIYAWK 242
Query: 14 LKL 6
+KL
Sbjct: 243 IKL 245
>dbj|BAM44344.1| berberine bridge enzyme [Coptis japonica]
Length = 533
Score = 187 bits (475), Expect = 3e-45
Identities = 99/233 (42%), Positives = 143/233 (61%), Gaps = 1/233 (0%)
Frame = -1
Query: 701 STMIMLALLNVWSFARSQDVDSLQSCLTAGGLTNVSTHADFNR-YDYILKYSLQNLRYTE 525
ST+++L L N+ SQ D L SCL G++N +T N YD +L S+QN +T
Sbjct: 9 STLMLLFLCNLTC---SQSEDDLPSCLILHGVSNYTTPFISNSDYDRLLYVSMQNQIFTR 65
Query: 524 RWVPKPALIILPMKKEEVPAAVKCCVETGWMMCIRSGGDDFEGLSSWADVPFVIVDLMNL 345
P+P++IILP K+++ + CC W + +RSGG +EGLS AD PFVI+D+MNL
Sbjct: 66 STFPRPSVIILPESKDQLVNTISCCRRGSWTIRLRSGGHSYEGLSHTADSPFVIIDMMNL 125
Query: 344 VSITVDIDTETAWAEAGATIGQLYAAIGNATDELAFAAGDCPTVXXXXXXXXXXXGSYHR 165
+++D+DT+TAWAE+GAT+G++Y AIG ++D L F+AG CPTV G R
Sbjct: 126 NGVSIDLDTQTAWAESGATLGEIYHAIGVSSDVLGFSAGYCPTVGSGGHISGGGFGMMSR 185
Query: 164 AFGLASDSVDDVIMVDAKGDLLNKTNMTEDVYWAXXXXXXXXXXXXVAWKLKL 6
+GLA+D+V D I++ A G L ++ +M EDV+WA AWKL+L
Sbjct: 186 KYGLAADNVVDAILISANGALYDRKSMGEDVFWAIRGGGGGVWGVVYAWKLQL 238
>gb|AAU20769.1| berberine bridge enzyme [Thalictrum flavum subsp. glaucum]
Length = 535
Score = 181 bits (458), Expect = 3e-43
Identities = 96/232 (41%), Positives = 140/232 (60%), Gaps = 3/232 (1%)
Frame = -1
Query: 692 IMLALLNVWSFARSQDVDSLQSCLTAGGLTNVSTH---ADFNRYDYILKYSLQNLRYTER 522
I+L LN ++ A D L SCLT G+ N +TH ++ + Y +L S+QN +T
Sbjct: 6 ILLVFLNNFTCAIIDD--DLPSCLTIHGVHNYTTHQSTSNSDAYHRLLYVSMQNQIFTRS 63
Query: 521 WVPKPALIILPMKKEEVPAAVKCCVETGWMMCIRSGGDDFEGLSSWADVPFVIVDLMNLV 342
P+P +IILP +++ + CC W + +RSGG +EGLS AD PFVI+DLMNL
Sbjct: 64 TFPQPRVIILPESMDQLANVISCCTRGSWTIRLRSGGHSYEGLSHIADNPFVIIDLMNLN 123
Query: 341 SITVDIDTETAWAEAGATIGQLYAAIGNATDELAFAAGDCPTVXXXXXXXXXXXGSYHRA 162
I++D+DT+TAW E+GAT+G++Y AIG ++ +AF+AG CPT G R
Sbjct: 124 GISIDLDTQTAWVESGATLGEIYHAIGKSSGTMAFSAGYCPTGGSGGHIAPGGFGMMSRK 183
Query: 161 FGLASDSVDDVIMVDAKGDLLNKTNMTEDVYWAXXXXXXXXXXXXVAWKLKL 6
+GLA+D+V D ++VDA G +L++ +M EDV+WA AWKL+L
Sbjct: 184 YGLAADNVVDALLVDANGVVLDRESMGEDVFWAIRGGGGGVWGAVYAWKLQL 235
>gb|AGL44333.1| FAD-dependent oxidoreductase, partial [Papaver somniferum]
Length = 535
Score = 179 bits (454), Expect = 8e-43
Identities = 94/221 (42%), Positives = 132/221 (59%), Gaps = 4/221 (1%)
Frame = -1
Query: 656 RSQDVDS--LQSCLTAGGLTNVSTHADFNRYDY--ILKYSLQNLRYTERWVPKPALIILP 489
R DV+ L SCL + G+ N +T + DY +L S+QN + + V KP+ I++P
Sbjct: 22 RGGDVNDNLLSSCLNSHGVHNFTTLSTDTNSDYFKLLHASIQNPLFAKPTVSKPSFIVMP 81
Query: 488 MKKEEVPAAVKCCVETGWMMCIRSGGDDFEGLSSWADVPFVIVDLMNLVSITVDIDTETA 309
KEE+ V CC W + +RSGG +EGLS AD PFVIVD+MNL I++D+++ETA
Sbjct: 82 GSKEELSRTVHCCTRESWTIRLRSGGHSYEGLSYTADTPFVIVDMMNLNRISIDVESETA 141
Query: 308 WAEAGATIGQLYAAIGNATDELAFAAGDCPTVXXXXXXXXXXXGSYHRAFGLASDSVDDV 129
W E+GAT+G+LY AI +T+ L F AG CPTV G R +GLA+D+V D
Sbjct: 142 WVESGATLGELYYAIAQSTETLGFTAGWCPTVGSGGHISGGGFGMMSRKYGLAADNVVDA 201
Query: 128 IMVDAKGDLLNKTNMTEDVYWAXXXXXXXXXXXXVAWKLKL 6
I++D+ G +L++ M EDV+WA AWK+KL
Sbjct: 202 ILIDSTGSILDREKMGEDVFWAIRGGGGGVWGAVYAWKIKL 242
>gb|AAD17487.1| berberine bridge enzyme [Berberis stolonifera]
Length = 536
Score = 179 bits (453), Expect = 1e-42
Identities = 93/214 (43%), Positives = 137/214 (64%), Gaps = 3/214 (1%)
Frame = -1
Query: 638 SLQSCLTAGGLTN---VSTHADFNRYDYILKYSLQNLRYTERWVPKPALIILPMKKEEVP 468
+L SCLT+ G++N +ST +D + Y +L S+QN +T P+P++IILP KEE+
Sbjct: 22 NLSSCLTSNGVSNFTALSTSSDSD-YHRLLYVSMQNQIFTRPKYPRPSMIILPQSKEELA 80
Query: 467 AAVKCCVETGWMMCIRSGGDDFEGLSSWADVPFVIVDLMNLVSITVDIDTETAWAEAGAT 288
A+V C W + +RSGG +EGLS AD PFV++DLMNL I++D++++TAW E+GAT
Sbjct: 81 ASVVCSNRGLWTIRLRSGGHSYEGLSYVADTPFVVIDLMNLNRISIDLESKTAWVESGAT 140
Query: 287 IGQLYAAIGNATDELAFAAGDCPTVXXXXXXXXXXXGSYHRAFGLASDSVDDVIMVDAKG 108
+G++Y AI A+D L F+ G CPTV G R +GLA+D+V D ++VDA G
Sbjct: 141 LGEIYCAISEASDTLGFSGGYCPTVGSGGHISGGGFGMMSRKYGLAADNVIDALIVDANG 200
Query: 107 DLLNKTNMTEDVYWAXXXXXXXXXXXXVAWKLKL 6
+L++++M EDV+WA AWKL+L
Sbjct: 201 AVLDRSSMGEDVFWAIRGGGGGVWGAIYAWKLQL 234
>sp|P93479.1|RETO_PAPSO RecName: Full=Reticuline oxidase; AltName: Full=Berberine
bridge-forming enzyme; Short=BBE; AltName:
Full=Tetrahydroprotoberberine synthase; Flags: Precursor
gi|3282517|gb|AAC61839.1| berberine bridge enzyme
[Papaver somniferum]
Length = 535
Score = 177 bits (450), Expect = 2e-42
Identities = 94/221 (42%), Positives = 132/221 (59%), Gaps = 4/221 (1%)
Frame = -1
Query: 656 RSQDVDS--LQSCLTAGGLTNVSTHADFNRYDY--ILKYSLQNLRYTERWVPKPALIILP 489
R DV+ L SCL + G+ N +T + DY +L S+QN + + V KP+ I++P
Sbjct: 22 RGGDVNDNLLSSCLNSHGVHNFTTLSTDTNSDYFKLLHASMQNPLFAKPTVSKPSFIVMP 81
Query: 488 MKKEEVPAAVKCCVETGWMMCIRSGGDDFEGLSSWADVPFVIVDLMNLVSITVDIDTETA 309
KEE+ + V CC W + +RSGG +EGLS AD PFVIVD+MNL I++D+ +ETA
Sbjct: 82 GSKEELSSTVHCCTRESWTIRLRSGGHSYEGLSYTADTPFVIVDMMNLNRISIDVLSETA 141
Query: 308 WAEAGATIGQLYAAIGNATDELAFAAGDCPTVXXXXXXXXXXXGSYHRAFGLASDSVDDV 129
W E+GAT+G+LY AI +TD L F AG CPTV G R +GLA+D+V D
Sbjct: 142 WVESGATLGELYYAIAQSTDTLGFTAGWCPTVGSGGHISGGGFGMMSRKYGLAADNVVDA 201
Query: 128 IMVDAKGDLLNKTNMTEDVYWAXXXXXXXXXXXXVAWKLKL 6
I++D+ G +L++ M +DV+WA AWK+KL
Sbjct: 202 ILIDSNGAILDREKMGDDVFWAIRGGGGGVWGAIYAWKIKL 242
>gb|ACO90226.1| putative berberine bridge enzyme [Papaver bracteatum]
Length = 259
Score = 176 bits (446), Expect = 7e-42
Identities = 93/231 (40%), Positives = 137/231 (59%), Gaps = 4/231 (1%)
Frame = -1
Query: 686 LALLNVWSFARSQDVDS--LQSCLTAGGLTNVSTHADFNRYDYI--LKYSLQNLRYTERW 519
L +++ + R DV+ L SCL + + N +T + +Y+ L S+QN + +
Sbjct: 3 LFFVSLQTCVRGGDVNDNLLLSCLNSHDVHNFTTLSTDTNSEYLKLLHASIQNPLFAKPT 62
Query: 518 VPKPALIILPMKKEEVPAAVKCCVETGWMMCIRSGGDDFEGLSSWADVPFVIVDLMNLVS 339
V KP+ I++P KEE+ + + CC W + +RSGG +EGLS AD PFVIVD+MNL
Sbjct: 63 VSKPSFIVIPGSKEELSSTIHCCTRESWTIRLRSGGHSYEGLSYTADTPFVIVDMMNLHG 122
Query: 338 ITVDIDTETAWAEAGATIGQLYAAIGNATDELAFAAGDCPTVXXXXXXXXXXXGSYHRAF 159
I++D ++ETAW E+GAT+G+LY AI +T+ L F AG CPTV G R +
Sbjct: 123 ISIDFESETAWVESGATLGELYYAIAQSTETLGFTAGWCPTVGSGGHISGGGFGMMSRKY 182
Query: 158 GLASDSVDDVIMVDAKGDLLNKTNMTEDVYWAXXXXXXXXXXXXVAWKLKL 6
GLA+D+V D I++D+KG +L++ M EDV+WA AWK+KL
Sbjct: 183 GLAADNVVDAILIDSKGAILDREKMGEDVFWAIRGGGGGVWGAVYAWKIKL 233
>gb|EXC32491.1| Reticuline oxidase [Morus notabilis]
Length = 557
Score = 173 bits (438), Expect = 6e-41
Identities = 97/247 (39%), Positives = 139/247 (56%), Gaps = 9/247 (3%)
Frame = -1
Query: 719 KLSFLQSTMIMLALLNVWSFARSQDVDSLQSCLTAGGLTNV-------STHADFNRYDY- 564
K + L ++ + ++V D + SCLT + N ST AD Y Y
Sbjct: 9 KATGLSLCLVFMCFMSVPLSLAGAPTDQISSCLTLHDVHNFTTFPDTSSTPADDYSYSYY 68
Query: 563 -ILKYSLQNLRYTERWVPKPALIILPMKKEEVPAAVKCCVETGWMMCIRSGGDDFEGLSS 387
+L +S+QNLR+ +PKP IILP +V +AV C + + + +RSGG +EG SS
Sbjct: 69 NMLNFSIQNLRFVAPSIPKPIAIILPHNVAQVTSAVLCSRKASYKIIVRSGGHSYEGTSS 128
Query: 386 WADVPFVIVDLMNLVSITVDIDTETAWAEAGATIGQLYAAIGNATDELAFAAGDCPTVXX 207
A PFVI+D+MNL IT+D++++TAW EAGAT+G+ Y AI A++ AF+AG CPTV
Sbjct: 129 IATPPFVIIDMMNLKQITLDLESKTAWVEAGATLGETYYAIAKASNVHAFSAGSCPTVGV 188
Query: 206 XXXXXXXXXGSYHRAFGLASDSVDDVIMVDAKGDLLNKTNMTEDVYWAXXXXXXXXXXXX 27
G R +GLA+D+V D ++VDA G LL++ M EDV+WA
Sbjct: 189 GGHIAGGGFGLLSRKYGLAADNVVDALLVDANGQLLDRHGMGEDVFWAIRGGGGGVWGIL 248
Query: 26 VAWKLKL 6
AWK++L
Sbjct: 249 YAWKIQL 255
>dbj|BAK18780.1| berberine bridge enzyme-like protein [Nicotiana tabacum]
Length = 562
Score = 171 bits (434), Expect = 2e-40
Identities = 92/217 (42%), Positives = 130/217 (59%), Gaps = 2/217 (0%)
Frame = -1
Query: 650 QDVDSLQSCLTAGGLTNVSTHADFNRYDYILKYSLQNLRYTERWVPKPALIILPMKKEEV 471
+ V +L +CL + N S + Y +L +SLQNLR+ +PKP +IILP KEE+
Sbjct: 25 EGVANLSTCLINHNVHNFSMYPTSRNYFNLLDFSLQNLRFAASNMPKPTVIILPNSKEEL 84
Query: 470 PAAVKCCVETGWMMCIRSGGDDFEGLSS--WADVPFVIVDLMNLVSITVDIDTETAWAEA 297
+ + CC +T + + +R GG +EG SS + PFVI+DLM L ++VD+D+ETAWA+
Sbjct: 85 VSTILCCRQTSYEIRVRCGGHSYEGTSSVSFDGSPFVIIDLMKLDDVSVDLDSETAWAQG 144
Query: 296 GATIGQLYAAIGNATDELAFAAGDCPTVXXXXXXXXXXXGSYHRAFGLASDSVDDVIMVD 117
GATIGQ+Y AI A+D AF+AG PTV G R FG+A+DSV D +++D
Sbjct: 145 GATIGQIYYAIAKASDVHAFSAGSGPTVGSGGHISGGGFGLLSRKFGVAADSVVDALLID 204
Query: 116 AKGDLLNKTNMTEDVYWAXXXXXXXXXXXXVAWKLKL 6
A G LL++ M EDV+WA AWK++L
Sbjct: 205 ADGRLLDRKAMGEDVFWAIRGGGGGNWGIIYAWKIRL 241
>pdb|3D2D|A Chain A, Structure Of Berberine Bridge Enzyme In Complex With
(S)-Reticuline gi|210060956|pdb|3D2H|A Chain A,
Structure Of Berberine Bridge Enzyme From Eschscholzia
Californica, Monoclinic Crystal Form
gi|210060957|pdb|3D2J|A Chain A, Structure Of Berberine
Bridge Enzyme From Eschscholzia Californica, Tetragonal
Crystal Form
Length = 538
Score = 171 bits (434), Expect = 2e-40
Identities = 93/237 (39%), Positives = 139/237 (58%), Gaps = 5/237 (2%)
Frame = -1
Query: 701 STMIMLALLNVWSFARSQDVDSLQSCLTAGGLTN-----VSTHADFNRYDYILKYSLQNL 537
S I L+LLN ++ + L SCLT G+ N + +DFNR+ L S+QN
Sbjct: 10 SLSIFLSLLNC-----AEAGNDLLSCLTFNGVRNHTVFSADSDSDFNRF---LHLSIQNP 61
Query: 536 RYTERWVPKPALIILPMKKEEVPAAVKCCVETGWMMCIRSGGDDFEGLSSWADVPFVIVD 357
+ + KP+ IILP KEE+ ++C + W + +RSGG +EGLS +D PF+++D
Sbjct: 62 LFQNSLISKPSAIILPGSKEELSNTIRCIRKGSWTIRLRSGGHSYEGLSYTSDTPFILID 121
Query: 356 LMNLVSITVDIDTETAWAEAGATIGQLYAAIGNATDELAFAAGDCPTVXXXXXXXXXXXG 177
LMNL +++D+++ETAW E+G+T+G+LY AI ++ +L F AG CPTV G
Sbjct: 122 LMNLNRVSIDLESETAWVESGSTLGELYYAITESSSKLGFTAGWCPTVGTGGHISGGGFG 181
Query: 176 SYHRAFGLASDSVDDVIMVDAKGDLLNKTNMTEDVYWAXXXXXXXXXXXXVAWKLKL 6
R +GLA+D+V D I++DA G +L++ M EDV+WA AWK+KL
Sbjct: 182 MMSRKYGLAADNVVDAILIDANGAILDRQAMGEDVFWAIRGGGGGVWGAIYAWKIKL 238
>sp|P30986.1|RETO_ESCCA RecName: Full=Reticuline oxidase; AltName: Full=Berberine
bridge-forming enzyme; Short=BBE; AltName:
Full=Tetrahydroprotoberberine synthase; Flags: Precursor
gi|239110|gb|AAB20352.1| (S)-reticuline:oxygen
oxidoreductase (methylene-bridge-forming) [Eschscholzia
californica] gi|2897944|gb|AAC39358.1| berberine bridge
enzyme [Eschscholzia californica]
Length = 538
Score = 170 bits (431), Expect = 4e-40
Identities = 93/237 (39%), Positives = 138/237 (58%), Gaps = 5/237 (2%)
Frame = -1
Query: 701 STMIMLALLNVWSFARSQDVDSLQSCLTAGGLTN-----VSTHADFNRYDYILKYSLQNL 537
S I L+LLN + + L SCLT G+ N + +DFNR+ L S+QN
Sbjct: 10 SLSIFLSLLNC-----ALGGNDLLSCLTFNGVRNHTVFSADSDSDFNRF---LHLSIQNP 61
Query: 536 RYTERWVPKPALIILPMKKEEVPAAVKCCVETGWMMCIRSGGDDFEGLSSWADVPFVIVD 357
+ + KP+ IILP KEE+ ++C + W + +RSGG +EGLS +D PF+++D
Sbjct: 62 LFQNSLISKPSAIILPGSKEELSNTIRCIRKGSWTIRLRSGGHSYEGLSYTSDTPFILID 121
Query: 356 LMNLVSITVDIDTETAWAEAGATIGQLYAAIGNATDELAFAAGDCPTVXXXXXXXXXXXG 177
LMNL +++D+++ETAW E+G+T+G+LY AI ++ +L F AG CPTV G
Sbjct: 122 LMNLNRVSIDLESETAWVESGSTLGELYYAITESSSKLGFTAGWCPTVGTGGHISGGGFG 181
Query: 176 SYHRAFGLASDSVDDVIMVDAKGDLLNKTNMTEDVYWAXXXXXXXXXXXXVAWKLKL 6
R +GLA+D+V D I++DA G +L++ M EDV+WA AWK+KL
Sbjct: 182 MMSRKYGLAADNVVDAILIDANGAILDRQAMGEDVFWAIRGGGGGVWGAIYAWKIKL 238
>gb|ACO90218.1| berberine bridge enzyme [Eschscholzia californica]
Length = 242
Score = 170 bits (431), Expect = 4e-40
Identities = 94/237 (39%), Positives = 138/237 (58%), Gaps = 5/237 (2%)
Frame = -1
Query: 701 STMIMLALLNVWSFARSQDVDSLQSCLTAGGLTN-----VSTHADFNRYDYILKYSLQNL 537
S I L+LLN + + L SCLT G+ N + +DFNR +L S+QN
Sbjct: 10 SLSIFLSLLNC-----ALGGNDLLSCLTFNGVRNHTVFSADSDSDFNR---LLHLSIQNP 61
Query: 536 RYTERWVPKPALIILPMKKEEVPAAVKCCVETGWMMCIRSGGDDFEGLSSWADVPFVIVD 357
+ + KP+ IILP KEE+ ++C + W + +RSGG +EGLS +D PF++VD
Sbjct: 62 LFQNSLISKPSAIILPGSKEELSNTIRCIRKGSWTIRLRSGGHSYEGLSYTSDTPFILVD 121
Query: 356 LMNLVSITVDIDTETAWAEAGATIGQLYAAIGNATDELAFAAGDCPTVXXXXXXXXXXXG 177
LMNL +++D+++ETAW E+G+T+G+LY AI ++ +L F AG CPTV G
Sbjct: 122 LMNLNRVSIDLESETAWVESGSTLGELYYAITESSSKLGFTAGWCPTVGTGGHISGGGFG 181
Query: 176 SYHRAFGLASDSVDDVIMVDAKGDLLNKTNMTEDVYWAXXXXXXXXXXXXVAWKLKL 6
R +GLA+D+V D I++DA G +L++ M EDV+WA AWK+KL
Sbjct: 182 MMSRKYGLAADNVVDAILIDANGAILDRQAMGEDVFWAIRGGGGGVWGAIYAWKIKL 238
>ref|XP_006846477.1| hypothetical protein AMTR_s00018p00124850 [Amborella trichopoda]
gi|548849287|gb|ERN08152.1| hypothetical protein
AMTR_s00018p00124850 [Amborella trichopoda]
Length = 526
Score = 169 bits (429), Expect = 7e-40
Identities = 86/230 (37%), Positives = 139/230 (60%), Gaps = 2/230 (0%)
Frame = -1
Query: 689 MLALLNVWSFARSQDVDSLQSCLTAGGLTNVSTHADFN--RYDYILKYSLQNLRYTERWV 516
+ L+++ FA +D LQSCL + G+ N ++ + + Y++ L +S+QNLR+T+ +
Sbjct: 9 LYTLISLLGFALGED---LQSCLLSNGVLNFTSRSQSSDAAYNHFLSFSIQNLRFTKPTM 65
Query: 515 PKPALIILPMKKEEVPAAVKCCVETGWMMCIRSGGDDFEGLSSWADVPFVIVDLMNLVSI 336
KP ++ILP ++ + + C ET + IR GG +EGLSS D F+I+DLMNL +I
Sbjct: 66 EKPVMVILPETHAQLSSTILCSKETSHEIRIRCGGHSYEGLSSTGDRSFIIIDLMNLNNI 125
Query: 335 TVDIDTETAWAEAGATIGQLYAAIGNATDELAFAAGDCPTVXXXXXXXXXXXGSYHRAFG 156
VD++T+TAW EAGAT+G+ Y +I AT + F+AG CPTV G R +G
Sbjct: 126 HVDLETQTAWVEAGATLGETYYSIARATSDFGFSAGSCPTVGSGGHISGGGFGFLSRKYG 185
Query: 155 LASDSVDDVIMVDAKGDLLNKTNMTEDVYWAXXXXXXXXXXXXVAWKLKL 6
LA+D+V D +++++ G+++ + M ED++WA AWK++L
Sbjct: 186 LAADNVLDTLVINSGGEVMEREAMGEDMFWALRGGGGGNWGLVYAWKIRL 235
>pdb|4EC3|A Chain A, Structure Of Berberine Bridge Enzyme, H174a Variant In
Complex With (S)-Reticuline
Length = 519
Score = 169 bits (428), Expect = 9e-40
Identities = 87/217 (40%), Positives = 130/217 (59%), Gaps = 5/217 (2%)
Frame = -1
Query: 641 DSLQSCLTAGGLTN-----VSTHADFNRYDYILKYSLQNLRYTERWVPKPALIILPMKKE 477
+ L SCLT G+ N + +DFNR+ L S+QN + + KP+ IILP KE
Sbjct: 6 NDLLSCLTFNGVRNHTVFSADSDSDFNRF---LHLSIQNPLFQNSLISKPSAIILPGSKE 62
Query: 476 EVPAAVKCCVETGWMMCIRSGGDDFEGLSSWADVPFVIVDLMNLVSITVDIDTETAWAEA 297
E+ ++C + W + +RSGG +EGLS +D PF+++DLMNL +++D+++ETAW E+
Sbjct: 63 ELSNTIRCIRKGSWTIRLRSGGHSYEGLSYTSDTPFILIDLMNLNRVSIDLESETAWVES 122
Query: 296 GATIGQLYAAIGNATDELAFAAGDCPTVXXXXXXXXXXXGSYHRAFGLASDSVDDVIMVD 117
G+T+G+LY AI ++ +L F AG CPTV G R +GLA+D+V D I++D
Sbjct: 123 GSTLGELYYAITESSSKLGFTAGWCPTVGTGGAISGGGFGMMSRKYGLAADNVVDAILID 182
Query: 116 AKGDLLNKTNMTEDVYWAXXXXXXXXXXXXVAWKLKL 6
A G +L++ M EDV+WA AWK+KL
Sbjct: 183 ANGAILDRQAMGEDVFWAIRGGGGGVWGAIYAWKIKL 219
>pdb|3GSY|A Chain A, Structure Of Berberine Bridge Enzyme In Complex With
Dehydroscoulerine
Length = 519
Score = 169 bits (428), Expect = 9e-40
Identities = 87/217 (40%), Positives = 130/217 (59%), Gaps = 5/217 (2%)
Frame = -1
Query: 641 DSLQSCLTAGGLTN-----VSTHADFNRYDYILKYSLQNLRYTERWVPKPALIILPMKKE 477
+ L SCLT G+ N + +DFNR+ L S+QN + + KP+ IILP KE
Sbjct: 6 NDLLSCLTFNGVRNHTVFSADSDSDFNRF---LHLSIQNPLFQNSLISKPSAIILPGSKE 62
Query: 476 EVPAAVKCCVETGWMMCIRSGGDDFEGLSSWADVPFVIVDLMNLVSITVDIDTETAWAEA 297
E+ ++C + W + +RSGG +EGLS +D PF+++DLMNL +++D+++ETAW E+
Sbjct: 63 ELSNTIRCIRKGSWTIRLRSGGHSYEGLSYTSDTPFILIDLMNLNRVSIDLESETAWVES 122
Query: 296 GATIGQLYAAIGNATDELAFAAGDCPTVXXXXXXXXXXXGSYHRAFGLASDSVDDVIMVD 117
G+T+G+LY AI ++ +L F AG CPTV G R +GLA+D+V D I++D
Sbjct: 123 GSTLGELYYAITESSSKLGFTAGWCPTVGTGGHISGGGFGMMSRKYGLAADNVVDAILID 182
Query: 116 AKGDLLNKTNMTEDVYWAXXXXXXXXXXXXVAWKLKL 6
A G +L++ M EDV+WA AWK+KL
Sbjct: 183 ANGAILDRQAMGEDVFWAIRGGGGGVWGAIYAWKIKL 219
>dbj|BAK18779.1| berberine bridge enzyme-like protein [Nicotiana tabacum]
Length = 559
Score = 169 bits (427), Expect = 1e-39
Identities = 91/215 (42%), Positives = 127/215 (59%), Gaps = 2/215 (0%)
Frame = -1
Query: 644 VDSLQSCLTAGGLTNVSTHADFNRYDYILKYSLQNLRYTERWVPKPALIILPMKKEEVPA 465
V +L +CL + N S + Y +L +SLQNLR+ ++PKP IILP KEE+ +
Sbjct: 23 VTNLSACLINHNVHNFSIYPTSRNYFNLLHFSLQNLRFAAPFMPKPTFIILPSSKEELVS 82
Query: 464 AVKCCVETGWMMCIRSGGDDFEGLS--SWADVPFVIVDLMNLVSITVDIDTETAWAEAGA 291
+ CC + + + +R GG +EG S S+ PFVIVDLM L ++VD+D+ETAWA+ GA
Sbjct: 83 TIFCCRKASYEIRVRCGGHSYEGTSYVSFDASPFVIVDLMKLDDVSVDLDSETAWAQGGA 142
Query: 290 TIGQLYAAIGNATDELAFAAGDCPTVXXXXXXXXXXXGSYHRAFGLASDSVDDVIMVDAK 111
TIGQ+Y AI +D AF+AG PTV G R FGLA+D+V D +++DA
Sbjct: 143 TIGQIYYAIAKVSDVHAFSAGSGPTVGSGGHISGGGFGLLSRKFGLAADNVVDALLIDAD 202
Query: 110 GDLLNKTNMTEDVYWAXXXXXXXXXXXXVAWKLKL 6
G LL++ M EDV+WA AWK++L
Sbjct: 203 GRLLDRKAMGEDVFWAIRGGGGGNWGIVYAWKIRL 237
>pdb|3FW9|A Chain A, Structure Of Berberine Bridge Enzyme In Complex With
(S)-Scoulerine
Length = 495
Score = 169 bits (427), Expect = 1e-39
Identities = 87/215 (40%), Positives = 129/215 (60%), Gaps = 5/215 (2%)
Frame = -1
Query: 635 LQSCLTAGGLTN-----VSTHADFNRYDYILKYSLQNLRYTERWVPKPALIILPMKKEEV 471
L SCLT G+ N + +DFNR+ L S+QN + + KP+ IILP KEE+
Sbjct: 2 LLSCLTFNGVRNHTVFSADSDSDFNRF---LHLSIQNPLFQNSLISKPSAIILPGSKEEL 58
Query: 470 PAAVKCCVETGWMMCIRSGGDDFEGLSSWADVPFVIVDLMNLVSITVDIDTETAWAEAGA 291
++C + W + +RSGG +EGLS +D PF+++DLMNL +++D+++ETAW E+G+
Sbjct: 59 SNTIRCIRKGSWTIRLRSGGHSYEGLSYTSDTPFILIDLMNLNRVSIDLESETAWVESGS 118
Query: 290 TIGQLYAAIGNATDELAFAAGDCPTVXXXXXXXXXXXGSYHRAFGLASDSVDDVIMVDAK 111
T+G+LY AI ++ +L F AG CPTV G R +GLA+D+V D I++DA
Sbjct: 119 TLGELYYAITESSSKLGFTAGWCPTVGTGGHISGGGFGMMSRKYGLAADNVVDAILIDAN 178
Query: 110 GDLLNKTNMTEDVYWAXXXXXXXXXXXXVAWKLKL 6
G +L++ M EDV+WA AWK+KL
Sbjct: 179 GAILDRQAMGEDVFWAIRGGGGGVWGAIYAWKIKL 213
>pdb|3FW7|A Chain A, Structure Of Berberine Bridge Enzyme, H104a Variant
Length = 498
Score = 169 bits (427), Expect = 1e-39
Identities = 87/217 (40%), Positives = 130/217 (59%), Gaps = 5/217 (2%)
Frame = -1
Query: 641 DSLQSCLTAGGLTN-----VSTHADFNRYDYILKYSLQNLRYTERWVPKPALIILPMKKE 477
+ L SCLT G+ N + +DFNR+ L S+QN + + KP+ IILP KE
Sbjct: 3 NDLLSCLTFNGVRNHTVFSADSDSDFNRF---LHLSIQNPLFQNSLISKPSAIILPGSKE 59
Query: 476 EVPAAVKCCVETGWMMCIRSGGDDFEGLSSWADVPFVIVDLMNLVSITVDIDTETAWAEA 297
E+ ++C + W + +RSGG +EGLS +D PF+++DLMNL +++D+++ETAW E+
Sbjct: 60 ELSNTIRCIRKGSWTIRLRSGGASYEGLSYTSDTPFILIDLMNLNRVSIDLESETAWVES 119
Query: 296 GATIGQLYAAIGNATDELAFAAGDCPTVXXXXXXXXXXXGSYHRAFGLASDSVDDVIMVD 117
G+T+G+LY AI ++ +L F AG CPTV G R +GLA+D+V D I++D
Sbjct: 120 GSTLGELYYAITESSSKLGFTAGWCPTVGTGGHISGGGFGMMSRKYGLAADNVVDAILID 179
Query: 116 AKGDLLNKTNMTEDVYWAXXXXXXXXXXXXVAWKLKL 6
A G +L++ M EDV+WA AWK+KL
Sbjct: 180 ANGAILDRQAMGEDVFWAIRGGGGGVWGAIYAWKIKL 216
>ref|XP_004158082.1| PREDICTED: reticuline oxidase-like protein-like [Cucumis sativus]
Length = 567
Score = 168 bits (426), Expect = 1e-39
Identities = 92/226 (40%), Positives = 130/226 (57%), Gaps = 6/226 (2%)
Frame = -1
Query: 665 SFARSQDVDSLQSCLTAGGLTNVSTHADF------NRYDYILKYSLQNLRYTERWVPKPA 504
SF +Q+ +S CL G ++ + A+ + Y IL +S+QNLR+ + +PKP
Sbjct: 30 SFCSTQESESFVQCLFDYGSSSTNPIANLIYTPTNSSYSSILNFSIQNLRFLNKDIPKPF 89
Query: 503 LIILPMKKEEVPAAVKCCVETGWMMCIRSGGDDFEGLSSWADVPFVIVDLMNLVSITVDI 324
II P+ + AAV C G+ + RSGG DFEGLS +D+PFV+VDL+NL SI++D+
Sbjct: 90 AIITPLHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISDIPFVLVDLINLKSISIDV 149
Query: 323 DTETAWAEAGATIGQLYAAIGNATDELAFAAGDCPTVXXXXXXXXXXXGSYHRAFGLASD 144
+ + AW +GAT+G+LY I + LAF AG CPTV G R +GLA+D
Sbjct: 150 ENQNAWVHSGATLGELYYRISEKSQTLAFPAGSCPTVGVGGHLSGGGYGWLIRKYGLAAD 209
Query: 143 SVDDVIMVDAKGDLLNKTNMTEDVYWAXXXXXXXXXXXXVAWKLKL 6
V D +VDA G + NK +M ED++WA VAWK+KL
Sbjct: 210 HVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGGSFGIVVAWKVKL 255