BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Ephedra27_contig00020093
         (1496 letters)

Database: ./nr 
           37,332,560 sequences; 13,225,080,153 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002984168.1| hypothetical protein SELMODRAFT_119619 [Sela...   597   e-168
ref|XP_002972254.1| hypothetical protein SELMODRAFT_97625 [Selag...   596   e-168
gb|AEO09337.4| alpha-amylase [Musa acuminata AAA Group]               546   e-152
ref|XP_001754460.1| predicted protein [Physcomitrella patens] gi...   544   e-152
ref|XP_001777583.1| predicted protein [Physcomitrella patens] gi...   543   e-152
gb|AAO11776.1| alpha-amylase precursor [Musa acuminata]               539   e-150
ref|XP_002282184.1| PREDICTED: alpha-amylase [Vitis vinifera] gi...   536   e-149
ref|NP_001062023.1| Os08g0473600 [Oryza sativa Japonica Group] g...   535   e-149
pir||JC7138 alpha-amylase (EC 3.2.1.1) isozyme III - rice             535   e-149
gb|AAN01149.1| alpha-amylase precursor [Musa acuminata]               534   e-149
ref|XP_006659493.1| PREDICTED: alpha-amylase isozyme 3E-like [Or...   532   e-148
ref|XP_004957017.1| PREDICTED: alpha-amylase isozyme 3C-like [Se...   532   e-148
ref|XP_004957016.1| PREDICTED: alpha-amylase isozyme 3C-like [Se...   532   e-148
ref|XP_004957015.1| PREDICTED: alpha-amylase isozyme 3C-like [Se...   531   e-148
gb|AAA98615.1| alpha-amylase [Hordeum vulgare subsp. vulgare]         531   e-148
emb|CAX51374.1| alpha-amylase [Hordeum vulgare subsp. vulgare] g...   531   e-148
emb|CAX51373.1| alpha-amylase [Hordeum vulgare subsp. vulgare]        530   e-148
sp|P04063.3|AMY2_HORVU RecName: Full=Alpha-amylase type B isozym...   530   e-148
gb|EMT32633.1| Alpha-amylase type B isozyme [Aegilops tauschii]       529   e-147
pdb|1AVA|A Chain A, Amy2BASI PROTEIN-Protein Complex From Barley...   529   e-147

>ref|XP_002984168.1| hypothetical protein SELMODRAFT_119619 [Selaginella moellendorffii]
            gi|300148017|gb|EFJ14678.1| hypothetical protein
            SELMODRAFT_119619 [Selaginella moellendorffii]
          Length = 407

 Score =  597 bits (1540), Expect = e-168
 Identities = 261/406 (64%), Positives = 325/406 (80%)
 Frame = -2

Query: 1333 IMLQGFNWESWRTKPSWYSVLKGKAKEISETGVTDVWMPPPSHSVAPQGYMPGRLYDLDA 1154
            +  QGFNWESWR  P WY VL   A+++++ GVTD+W+PP +HSVAPQGYMPGRLYDL+A
Sbjct: 5    VAFQGFNWESWRN-PCWYDVLMNTAEDLAQAGVTDIWLPPATHSVAPQGYMPGRLYDLNA 63

Query: 1153 SAYGKEQELKDLIDHLHKTGVRCMADIVINHRCGDAQDSRGVWCVFEGGTPDDRLDWGPW 974
            S YG E++LK +ID  H  G+RC+ADIVINHRCGD+QD RGVWC+FEGGTPD+RLDWGPW
Sbjct: 64   SKYGNEEKLKQVIDKFHSHGIRCIADIVINHRCGDSQDQRGVWCIFEGGTPDERLDWGPW 123

Query: 973  AIVKDDHPYTDGRGNHDTGDDFGAAPDIDHTNPRVQNDISEWLAWLKCDVGFDGWRFDFA 794
            AI KDD+ Y+DG G  DTG+DFGAAPDIDHTNPRVQ+D++ W+ W+K  +GFDGWRFDF+
Sbjct: 124  AITKDDYAYSDGSGAPDTGEDFGAAPDIDHTNPRVQDDLAGWMKWMKETIGFDGWRFDFS 183

Query: 793  KGYSPEYVRLYVERTFGDPRTSLCVGEIWNSLRYGHDGRPEYEQGQHREEILNWVRGTGG 614
            KGY+  Y  LY+ERT         VGE+W +L YG DG  EY Q  HR+E+++W+  TG 
Sbjct: 184  KGYAGSYAGLYIERT----TPEFSVGELWTNLNYGPDGSVEYNQDSHRQEVVDWINATGN 239

Query: 613  LCSAFDFTTKGILQEALGASELWRLRDSSMSPPGVIGAWPEKAVTFIDNHDTGSTQGHWA 434
              +AFDFTTKGILQEA+  ++ WRLRD +  P GVIG WP+KAVTFIDNHDTGSTQ HW 
Sbjct: 240  RSTAFDFTTKGILQEAV-KNQFWRLRDPNNKPAGVIGYWPQKAVTFIDNHDTGSTQKHWP 298

Query: 433  FPSDKVMLGYAYILLHPGVPCIFYDHLFDWGIGDGIKKLVDVRRRNGVKANSKCEILAAE 254
            FP+DKVMLGY YIL HPG+PCIFYDH ++WG+ D IK L++VR+RN V ANS   I+AAE
Sbjct: 299  FPADKVMLGYVYILTHPGIPCIFYDHYYEWGLKDEIKALLEVRKRNNVVANSSVSIIAAE 358

Query: 253  ADLYMASVNDGKVIVKIGPRFDIGGLAPTSERFRVAATGHDFCVWE 116
             D+Y+ASV DGK++VK+GPRFDIG +AP+S+ F +AA G+++CVWE
Sbjct: 359  EDVYVASVADGKLVVKMGPRFDIGDMAPSSDEFAIAALGNEYCVWE 404


>ref|XP_002972254.1| hypothetical protein SELMODRAFT_97625 [Selaginella moellendorffii]
            gi|300159721|gb|EFJ26340.1| hypothetical protein
            SELMODRAFT_97625 [Selaginella moellendorffii]
          Length = 407

 Score =  596 bits (1537), Expect = e-168
 Identities = 260/406 (64%), Positives = 324/406 (79%)
 Frame = -2

Query: 1333 IMLQGFNWESWRTKPSWYSVLKGKAKEISETGVTDVWMPPPSHSVAPQGYMPGRLYDLDA 1154
            +  QGFNWESWR  P WY VL   A+++++ GVTD+W+PP +HSVAPQGYMPGRLYDL+A
Sbjct: 5    VAFQGFNWESWRN-PCWYDVLMNTAEDLAQAGVTDIWLPPATHSVAPQGYMPGRLYDLNA 63

Query: 1153 SAYGKEQELKDLIDHLHKTGVRCMADIVINHRCGDAQDSRGVWCVFEGGTPDDRLDWGPW 974
            S YG E++LK +ID  H  G+RC+ADIVINHRCGD+QD RGVWC+FEGGTPD+RLDWGPW
Sbjct: 64   SKYGNEEKLKQVIDKFHSHGIRCIADIVINHRCGDSQDQRGVWCIFEGGTPDERLDWGPW 123

Query: 973  AIVKDDHPYTDGRGNHDTGDDFGAAPDIDHTNPRVQNDISEWLAWLKCDVGFDGWRFDFA 794
            AI KDD+ Y+DG G  DTG+DFGAAPDIDHTNPRVQ+D++ W+ W+K  +GFDGWRFDF+
Sbjct: 124  AITKDDYAYSDGSGAPDTGEDFGAAPDIDHTNPRVQDDLAGWMKWMKETIGFDGWRFDFS 183

Query: 793  KGYSPEYVRLYVERTFGDPRTSLCVGEIWNSLRYGHDGRPEYEQGQHREEILNWVRGTGG 614
            KGY+  Y  LY+ERT         VGE W +L YG DG  EY Q  HR+E+++W+  TG 
Sbjct: 184  KGYAGSYTGLYIERT----TPEFSVGEFWTNLNYGPDGSVEYNQDSHRQEVVDWINATGN 239

Query: 613  LCSAFDFTTKGILQEALGASELWRLRDSSMSPPGVIGAWPEKAVTFIDNHDTGSTQGHWA 434
              +AFDFTTKGILQEA+  ++ WRLRD +  P GVIG WP+KAVTF+DNHDTGSTQ HW 
Sbjct: 240  RSTAFDFTTKGILQEAV-KNQFWRLRDPNNKPAGVIGYWPQKAVTFVDNHDTGSTQKHWP 298

Query: 433  FPSDKVMLGYAYILLHPGVPCIFYDHLFDWGIGDGIKKLVDVRRRNGVKANSKCEILAAE 254
            FP+DKVMLGY YIL HPG+PCIFYDH ++WG+ D IK L++VR+RN V ANS   I+AAE
Sbjct: 299  FPADKVMLGYVYILTHPGIPCIFYDHYYEWGLKDEIKALLEVRKRNNVVANSSVSIIAAE 358

Query: 253  ADLYMASVNDGKVIVKIGPRFDIGGLAPTSERFRVAATGHDFCVWE 116
             D+Y+ASV DGK++VK+GPRFDIG +AP+S+ F +AA G+++CVWE
Sbjct: 359  EDVYVASVADGKLVVKMGPRFDIGDMAPSSDEFAIAALGNEYCVWE 404


>gb|AEO09337.4| alpha-amylase [Musa acuminata AAA Group]
          Length = 416

 Score =  546 bits (1406), Expect = e-152
 Identities = 248/410 (60%), Positives = 308/410 (75%)
 Frame = -2

Query: 1345 ALEAIMLQGFNWESWRTKPSWYSVLKGKAKEISETGVTDVWMPPPSHSVAPQGYMPGRLY 1166
            A   I+ QGFNWESWR +  WY+ LK K  +I+  GVT VW+PPPSHSV  QGYMPGRLY
Sbjct: 13   AQSQILFQGFNWESWRQQGGWYNFLKDKVSDIANAGVTHVWLPPPSHSVGVQGYMPGRLY 72

Query: 1165 DLDASAYGKEQELKDLIDHLHKTGVRCMADIVINHRCGDAQDSRGVWCVFEGGTPDDRLD 986
            DL AS YG + ELK LI   H  GV+C+ADIVINHRC D QD RG+WC+FEGGT D RLD
Sbjct: 73   DLGASKYGNQDELKALIGAFHDKGVKCVADIVINHRCADKQDGRGIWCIFEGGTDDARLD 132

Query: 985  WGPWAIVKDDHPYTDGRGNHDTGDDFGAAPDIDHTNPRVQNDISEWLAWLKCDVGFDGWR 806
            WGP  I +DD  Y+DG GN DTG+ F AAPDIDH N +VQ ++++WL WLK D+GFDGWR
Sbjct: 133  WGPHMICRDDTQYSDGTGNLDTGEGFAAAPDIDHLNTQVQRELTDWLNWLKTDIGFDGWR 192

Query: 805  FDFAKGYSPEYVRLYVERTFGDPRTSLCVGEIWNSLRYGHDGRPEYEQGQHREEILNWVR 626
             DFAKGYS    ++YVE+T    + +  V EIW+SL YG+DG+P Y+Q  +R+E++NWV+
Sbjct: 193  LDFAKGYSSSIAKIYVEQT----QPNFVVAEIWSSLAYGNDGKPAYDQNGNRQELVNWVQ 248

Query: 625  GTGGLCSAFDFTTKGILQEALGASELWRLRDSSMSPPGVIGAWPEKAVTFIDNHDTGSTQ 446
              GG  +AFDFTTKGILQ A+   ELWR+RDS    PG++G WPEKAVTF+DNHDTGSTQ
Sbjct: 249  QVGGPATAFDFTTKGILQAAV-EGELWRMRDSQGKAPGMMGWWPEKAVTFVDNHDTGSTQ 307

Query: 445  GHWAFPSDKVMLGYAYILLHPGVPCIFYDHLFDWGIGDGIKKLVDVRRRNGVKANSKCEI 266
              W FPSDKVM GYAYIL HPGVP IFYDH+FDWG+ + I +L + R RNG+ + S   I
Sbjct: 308  RLWPFPSDKVMQGYAYILTHPGVPSIFYDHMFDWGLKEAITQLAETRTRNGIHSGSALNI 367

Query: 265  LAAEADLYMASVNDGKVIVKIGPRFDIGGLAPTSERFRVAATGHDFCVWE 116
            LA++ADLYMA + DGK++ K+G R+D+G L P++  F V A+G+D+CVWE
Sbjct: 368  LASDADLYMAMI-DGKILTKLGSRYDVGNLVPSN--FHVVASGNDYCVWE 414


>ref|XP_001754460.1| predicted protein [Physcomitrella patens] gi|162694562|gb|EDQ80910.1|
            predicted protein [Physcomitrella patens]
          Length = 397

 Score =  544 bits (1402), Expect = e-152
 Identities = 243/402 (60%), Positives = 306/402 (76%)
 Frame = -2

Query: 1321 GFNWESWRTKPSWYSVLKGKAKEISETGVTDVWMPPPSHSVAPQGYMPGRLYDLDASAYG 1142
            GFNWESW++ PSWY ++  +A+E++  G+TDVW PPPSHSVAPQGYMPGRLYDL AS YG
Sbjct: 1    GFNWESWKS-PSWYDIIGNRAEELAAAGITDVWFPPPSHSVAPQGYMPGRLYDLSASKYG 59

Query: 1141 KEQELKDLIDHLHKTGVRCMADIVINHRCGDAQDSRGVWCVFEGGTPDDRLDWGPWAIVK 962
             E++L + I+  HK GVRC+ADIV+NHRCGDAQD RG W +FEGGTPDD LDWGPWA+V 
Sbjct: 60   NEEKLFETINKFHKAGVRCIADIVVNHRCGDAQDERGEWVIFEGGTPDDALDWGPWAVVG 119

Query: 961  DDHPYTDGRGNHDTGDDFGAAPDIDHTNPRVQNDISEWLAWLKCDVGFDGWRFDFAKGYS 782
            DD+PY +G G  DTG DF AAPDIDHTN RVQ+DI  W+ W+K  +GFDGWRFDFAKGY 
Sbjct: 120  DDYPYGNGTGAPDTGADFEAAPDIDHTNERVQSDIINWMNWMKYKIGFDGWRFDFAKGYG 179

Query: 781  PEYVRLYVERTFGDPRTSLCVGEIWNSLRYGHDGRPEYEQGQHREEILNWVRGTGGLCSA 602
              +V  Y+E+T         VGE+W ++ YG+ G  +Y Q  HR+++++WV  T    +A
Sbjct: 180  GYFVGKYIEKT----EPGFAVGELWTNMCYGYGGL-DYNQDGHRQQLVDWVHSTHNRSTA 234

Query: 601  FDFTTKGILQEALGASELWRLRDSSMSPPGVIGAWPEKAVTFIDNHDTGSTQGHWAFPSD 422
            FDFTTKGILQEA+   +LWRLRD +  PPG+IG WPEKAVTF+DNHDTGSTQ HW FPS+
Sbjct: 235  FDFTTKGILQEAV-KGQLWRLRDRNSKPPGMIGYWPEKAVTFLDNHDTGSTQNHWPFPSE 293

Query: 421  KVMLGYAYILLHPGVPCIFYDHLFDWGIGDGIKKLVDVRRRNGVKANSKCEILAAEADLY 242
             +M GYAYIL H G PCIFYDH +DWG+ D I +L+++R+RN + A SK  I+ AE DLY
Sbjct: 294  HIMQGYAYILTHSGNPCIFYDHFYDWGLKDEITRLIELRKRNDINARSKVHIVTAENDLY 353

Query: 241  MASVNDGKVIVKIGPRFDIGGLAPTSERFRVAATGHDFCVWE 116
            +A ++D  VI+KIGPR+D+G L P S  +++ A G D+CVWE
Sbjct: 354  VAKIDD-CVILKIGPRYDMGHLTPNSGDYKIGAVGKDYCVWE 394


>ref|XP_001777583.1| predicted protein [Physcomitrella patens] gi|162671068|gb|EDQ57626.1|
            predicted protein [Physcomitrella patens]
          Length = 412

 Score =  543 bits (1399), Expect = e-152
 Identities = 241/406 (59%), Positives = 305/406 (75%)
 Frame = -2

Query: 1333 IMLQGFNWESWRTKPSWYSVLKGKAKEISETGVTDVWMPPPSHSVAPQGYMPGRLYDLDA 1154
            ++L GFNWESW++   WY V+   A++++  G+TDVW PP SHSV+PQGYMPGRLYDL+ 
Sbjct: 12   LLLVGFNWESWKSS-CWYDVMGETAEDLAAAGITDVWFPPSSHSVSPQGYMPGRLYDLND 70

Query: 1153 SAYGKEQELKDLIDHLHKTGVRCMADIVINHRCGDAQDSRGVWCVFEGGTPDDRLDWGPW 974
              YG E++L++ I+  H+ GVRC+ADIV+NHRCG+ QD RG W +FEGGTPDD LDWGPW
Sbjct: 71   CKYGNEEKLRETIEKFHRVGVRCIADIVVNHRCGEEQDERGEWVIFEGGTPDDALDWGPW 130

Query: 973  AIVKDDHPYTDGRGNHDTGDDFGAAPDIDHTNPRVQNDISEWLAWLKCDVGFDGWRFDFA 794
            AIV DD+PY +G G  DTGDDF AAPDIDHTN  VQ+D+  W+ W+K  +GFDGWRFDFA
Sbjct: 131  AIVGDDYPYGNGTGAPDTGDDFEAAPDIDHTNDIVQSDLIVWMNWMKFKIGFDGWRFDFA 190

Query: 793  KGYSPEYVRLYVERTFGDPRTSLCVGEIWNSLRYGHDGRPEYEQGQHREEILNWVRGTGG 614
            KGY   +V  Y+ +T         VGE W SL YGHDG  EY Q  HR+++++W+  T  
Sbjct: 191  KGYGGYFVGRYIRKT----EPQFAVGEFWTSLNYGHDGL-EYNQDSHRQQLVDWIHATKE 245

Query: 613  LCSAFDFTTKGILQEALGASELWRLRDSSMSPPGVIGAWPEKAVTFIDNHDTGSTQGHWA 434
              +AFDFTTKGILQEA+   +LWRLRD +  PPG+IG WP KAVTF+DNHDTGSTQGHW 
Sbjct: 246  RSTAFDFTTKGILQEAV-KGQLWRLRDPNSKPPGLIGYWPSKAVTFLDNHDTGSTQGHWP 304

Query: 433  FPSDKVMLGYAYILLHPGVPCIFYDHLFDWGIGDGIKKLVDVRRRNGVKANSKCEILAAE 254
            FP + +M GYAYIL HPG PCIFYDH +DWG+ + IK L+DVR+RN + A SK  I  AE
Sbjct: 305  FPGEHIMQGYAYILTHPGNPCIFYDHFYDWGLKEEIKTLIDVRKRNDINAKSKVHICCAE 364

Query: 253  ADLYMASVNDGKVIVKIGPRFDIGGLAPTSERFRVAATGHDFCVWE 116
             DLY+A ++D +VI+K+GPR+DIG LAP  + +++AA G D+CVWE
Sbjct: 365  HDLYVAKIDD-RVILKMGPRYDIGDLAPNCDEYKIAAVGKDYCVWE 409


>gb|AAO11776.1| alpha-amylase precursor [Musa acuminata]
          Length = 416

 Score =  539 bits (1388), Expect = e-150
 Identities = 245/410 (59%), Positives = 305/410 (74%)
 Frame = -2

Query: 1345 ALEAIMLQGFNWESWRTKPSWYSVLKGKAKEISETGVTDVWMPPPSHSVAPQGYMPGRLY 1166
            A   I+ QGFNWESWR +  WY+ LK K  +I+  GVT VW+PPPSHSV  QGYMPGRLY
Sbjct: 13   AQSQILFQGFNWESWRQQGGWYNFLKDKVSDIANAGVTHVWLPPPSHSVGVQGYMPGRLY 72

Query: 1165 DLDASAYGKEQELKDLIDHLHKTGVRCMADIVINHRCGDAQDSRGVWCVFEGGTPDDRLD 986
            DL AS YG + ELK LI   H  GV+C+ADIVINHRC D QD RG+WC+FEGGT D RLD
Sbjct: 73   DLGASKYGNQDELKALIGAFHDKGVKCVADIVINHRCADKQDGRGIWCIFEGGTDDARLD 132

Query: 985  WGPWAIVKDDHPYTDGRGNHDTGDDFGAAPDIDHTNPRVQNDISEWLAWLKCDVGFDGWR 806
            WGP  I +DD  Y+DG GN DTG+ F AAPDIDH N +VQ ++++WL WLK D+GFDGWR
Sbjct: 133  WGPHMICRDDTQYSDGTGNLDTGEGFAAAPDIDHLNTQVQRELTDWLNWLKTDIGFDGWR 192

Query: 805  FDFAKGYSPEYVRLYVERTFGDPRTSLCVGEIWNSLRYGHDGRPEYEQGQHREEILNWVR 626
             DFA+GYS    ++YVE+T    + +  V EIWNSL YG+DG+P Y+Q  +R+ ++NWV+
Sbjct: 193  LDFAEGYSSSIAKIYVEQT----QPNFVVAEIWNSLAYGNDGKPAYDQNGNRQGLVNWVQ 248

Query: 625  GTGGLCSAFDFTTKGILQEALGASELWRLRDSSMSPPGVIGAWPEKAVTFIDNHDTGSTQ 446
              GG  +AFDFTTKGILQ A+   ELWR+RD     PG++G WPEKAVTF+DNHDTGSTQ
Sbjct: 249  QVGGPATAFDFTTKGILQAAV-EGELWRMRDPQGKAPGMMGWWPEKAVTFVDNHDTGSTQ 307

Query: 445  GHWAFPSDKVMLGYAYILLHPGVPCIFYDHLFDWGIGDGIKKLVDVRRRNGVKANSKCEI 266
              W FPSDKVM GYAYIL HPGVP IFYDH+FDWG+ + I +L   R RNG+ + S   I
Sbjct: 308  RLWPFPSDKVMQGYAYILTHPGVPSIFYDHMFDWGLKEKITRLAKTRTRNGIHSGSALNI 367

Query: 265  LAAEADLYMASVNDGKVIVKIGPRFDIGGLAPTSERFRVAATGHDFCVWE 116
            LA++ADLYMA + DGK++ K+G R+D+G L P++  F V ++G+D+CVWE
Sbjct: 368  LASDADLYMAMI-DGKILTKLGSRYDVGNLVPSN--FHVGSSGNDYCVWE 414


>ref|XP_002282184.1| PREDICTED: alpha-amylase [Vitis vinifera]
            gi|297741283|emb|CBI32414.3| unnamed protein product
            [Vitis vinifera]
          Length = 425

 Score =  536 bits (1380), Expect = e-149
 Identities = 242/406 (59%), Positives = 305/406 (75%)
 Frame = -2

Query: 1333 IMLQGFNWESWRTKPSWYSVLKGKAKEISETGVTDVWMPPPSHSVAPQGYMPGRLYDLDA 1154
            I+ QGFNWES + +  WY+ L     E++ +G+T VW+PPPS SV+P+GYMPGRLYDL+A
Sbjct: 25   ILFQGFNWESSKKEGGWYNFLINSIPELAASGITHVWLPPPSQSVSPEGYMPGRLYDLNA 84

Query: 1153 SAYGKEQELKDLIDHLHKTGVRCMADIVINHRCGDAQDSRGVWCVFEGGTPDDRLDWGPW 974
            S YG + ELK LI   H  GV+C+ADIVINHR  + QD+RG+W +FEGGTPDDRLDW P 
Sbjct: 85   SKYGTQDELKTLIKVFHSKGVQCIADIVINHRTAEKQDARGIWAIFEGGTPDDRLDWTPS 144

Query: 973  AIVKDDHPYTDGRGNHDTGDDFGAAPDIDHTNPRVQNDISEWLAWLKCDVGFDGWRFDFA 794
             I KDD PY+DG GN D+GDD+ AAPDIDH NPRVQ ++ +W+ WLK ++GFDGWRFDFA
Sbjct: 145  FICKDDTPYSDGTGNPDSGDDYSAAPDIDHINPRVQQELIDWMNWLKIEIGFDGWRFDFA 204

Query: 793  KGYSPEYVRLYVERTFGDPRTSLCVGEIWNSLRYGHDGRPEYEQGQHREEILNWVRGTGG 614
            +G+SP + + Y+ +T    R    VGEIW SL Y  DG+P Y Q  HR E++ WVRG GG
Sbjct: 205  RGFSPAFTKFYMAKT----RPKFAVGEIWKSLSYRSDGKPSYNQDSHRRELVEWVRGAGG 260

Query: 613  LCSAFDFTTKGILQEALGASELWRLRDSSMSPPGVIGAWPEKAVTFIDNHDTGSTQGHWA 434
              +AFDFTTKGILQ A+   ELWR++D +  PPG+IG  P  AVTFIDNHDTGST  HW 
Sbjct: 261  AVNAFDFTTKGILQAAV-EGELWRMKDLNGKPPGMIGLMPGNAVTFIDNHDTGSTLQHWP 319

Query: 433  FPSDKVMLGYAYILLHPGVPCIFYDHLFDWGIGDGIKKLVDVRRRNGVKANSKCEILAAE 254
            FPSDKVM GYAYIL HPG+P IFYDH F+WG+ + I KL+ +R RN +K NS   ILA++
Sbjct: 320  FPSDKVMQGYAYILTHPGIPSIFYDHFFEWGLKEEIMKLIIIRSRNRIKPNSAVRILASD 379

Query: 253  ADLYMASVNDGKVIVKIGPRFDIGGLAPTSERFRVAATGHDFCVWE 116
            +DLY+A++ DGK+IVKIGPRFD+G L P S + ++A +G D+CVWE
Sbjct: 380  SDLYVAAI-DGKIIVKIGPRFDVGNLVPKSFK-KIATSGKDYCVWE 423


>ref|NP_001062023.1| Os08g0473600 [Oryza sativa Japonica Group]
            gi|113683|sp|P27934.1|AMY3E_ORYSJ RecName:
            Full=Alpha-amylase isozyme 3E; AltName:
            Full=1,4-alpha-D-glucan glucanohydrolase; Flags:
            Precursor gi|169773|gb|AAA33896.1| alpha-amylase [Oryza
            sativa Japonica Group] gi|42407385|dbj|BAD09374.1|
            alpha-amylase isozyme 3E precursor [Oryza sativa Japonica
            Group] gi|42408198|dbj|BAD09334.1| alpha-amylase isozyme
            3E precursor [Oryza sativa Japonica Group]
            gi|113623992|dbj|BAF23937.1| Os08g0473600 [Oryza sativa
            Japonica Group] gi|125561873|gb|EAZ07321.1| hypothetical
            protein OsI_29569 [Oryza sativa Indica Group]
            gi|125603734|gb|EAZ43059.1| hypothetical protein
            OsJ_27648 [Oryza sativa Japonica Group]
            gi|194396091|gb|ACF60463.1| alpha amylase isozyme 3E
            [Oryza sativa Japonica Group]
            gi|215737139|dbj|BAG96068.1| unnamed protein product
            [Oryza sativa Japonica Group]
          Length = 437

 Score =  535 bits (1379), Expect = e-149
 Identities = 247/406 (60%), Positives = 300/406 (73%)
 Frame = -2

Query: 1333 IMLQGFNWESWRTKPSWYSVLKGKAKEISETGVTDVWMPPPSHSVAPQGYMPGRLYDLDA 1154
            ++ QGFNWESWR +  WY+ L  K +EI+ TG T VW+PPPSHSV+PQGYMPGRLYDLDA
Sbjct: 27   VLFQGFNWESWRKQGGWYNFLHEKVEEIASTGATHVWLPPPSHSVSPQGYMPGRLYDLDA 86

Query: 1153 SAYGKEQELKDLIDHLHKTGVRCMADIVINHRCGDAQDSRGVWCVFEGGTPDDRLDWGPW 974
            S YG E ELK LI+  H   V C+ADIVINHRC D +DSRGV+CVFEGGTPD RLDWGP 
Sbjct: 87   SKYGTEAELKSLIEAFHDKNVECLADIVINHRCADYKDSRGVYCVFEGGTPDGRLDWGPD 146

Query: 973  AIVKDDHPYTDGRGNHDTGDDFGAAPDIDHTNPRVQNDISEWLAWLKCDVGFDGWRFDFA 794
             I  DD  Y++GRG+ DTG  FGAAPDIDH NPRVQ ++++WL WL+ D+GFDGWR DFA
Sbjct: 147  MICSDDTQYSNGRGHRDTGAGFGAAPDIDHLNPRVQRELTDWLNWLRTDLGFDGWRLDFA 206

Query: 793  KGYSPEYVRLYVERTFGDPRTSLCVGEIWNSLRYGHDGRPEYEQGQHREEILNWVRGTGG 614
            KGYS    R+YV+ T      +  VGEIW+SL Y  DG+P   Q   R+E++NWV G G 
Sbjct: 207  KGYSAPLARIYVDNT----NPTFVVGEIWSSLIYNGDGKPSTNQDADRQELVNWVEGVGK 262

Query: 613  LCSAFDFTTKGILQEALGASELWRLRDSSMSPPGVIGAWPEKAVTFIDNHDTGSTQGHWA 434
              +AFDFTTKGILQ A+   ELWRL D +   PG++G  P++AVTF+DNHDTGSTQ  W 
Sbjct: 263  PATAFDFTTKGILQAAV-QGELWRLHDGNGKAPGLMGWMPDQAVTFVDNHDTGSTQSLWP 321

Query: 433  FPSDKVMLGYAYILLHPGVPCIFYDHLFDWGIGDGIKKLVDVRRRNGVKANSKCEILAAE 254
            FPSDKVM GYAYIL HPG+PCIFYDH+FDW +   I  L ++R RNG+ A S  +IL AE
Sbjct: 322  FPSDKVMQGYAYILTHPGIPCIFYDHVFDWNLQHEIATLAEIRSRNGIHAESTLDILKAE 381

Query: 253  ADLYMASVNDGKVIVKIGPRFDIGGLAPTSERFRVAATGHDFCVWE 116
             D+Y+A + DGKVI K+GPR+D GG+ P+   F V A G+D+CVWE
Sbjct: 382  GDIYVAMI-DGKVITKLGPRYDAGGIIPSD--FHVVAHGNDYCVWE 424


>pir||JC7138 alpha-amylase (EC 3.2.1.1) isozyme III - rice
          Length = 437

 Score =  535 bits (1379), Expect = e-149
 Identities = 247/406 (60%), Positives = 300/406 (73%)
 Frame = -2

Query: 1333 IMLQGFNWESWRTKPSWYSVLKGKAKEISETGVTDVWMPPPSHSVAPQGYMPGRLYDLDA 1154
            ++ QGFNWESWR +  WY+ L  K +EI+ TG T VW+PPPSHSV+PQGYMPGRLYDLDA
Sbjct: 27   VLFQGFNWESWRKQGGWYNFLHEKVEEIASTGATHVWLPPPSHSVSPQGYMPGRLYDLDA 86

Query: 1153 SAYGKEQELKDLIDHLHKTGVRCMADIVINHRCGDAQDSRGVWCVFEGGTPDDRLDWGPW 974
            S YG E ELK LI+  H   V C+ADIVINHRC D +DSRGV+CVFEGGTPD RLDWGP 
Sbjct: 87   SKYGTEAELKSLIEAFHDKNVECLADIVINHRCADYKDSRGVYCVFEGGTPDGRLDWGPD 146

Query: 973  AIVKDDHPYTDGRGNHDTGDDFGAAPDIDHTNPRVQNDISEWLAWLKCDVGFDGWRFDFA 794
             I  DD  Y++GRG+ DTG  FGAAPDIDH NPRVQ ++++WL WL+ D+GFDGWR DFA
Sbjct: 147  MICSDDTQYSNGRGHRDTGAGFGAAPDIDHLNPRVQRELTDWLNWLRTDLGFDGWRLDFA 206

Query: 793  KGYSPEYVRLYVERTFGDPRTSLCVGEIWNSLRYGHDGRPEYEQGQHREEILNWVRGTGG 614
            KGYS    R+YV+ T      +  VGEIW+SL Y  DG+P   Q   R+E++NWV G G 
Sbjct: 207  KGYSAPLARIYVDNT----NPTFVVGEIWSSLIYNGDGKPSTNQDADRQELVNWVEGVGK 262

Query: 613  LCSAFDFTTKGILQEALGASELWRLRDSSMSPPGVIGAWPEKAVTFIDNHDTGSTQGHWA 434
              +AFDFTTKGILQ A+   ELWRL D +   PG++G  P++AVTF+DNHDTGSTQ  W 
Sbjct: 263  PATAFDFTTKGILQAAV-QGELWRLHDGNGKAPGLMGWMPDQAVTFVDNHDTGSTQSLWP 321

Query: 433  FPSDKVMLGYAYILLHPGVPCIFYDHLFDWGIGDGIKKLVDVRRRNGVKANSKCEILAAE 254
            FPSDKVM GYAYIL HPG+PCIFYDH+FDW +   I  L ++R RNG+ A S  +IL AE
Sbjct: 322  FPSDKVMQGYAYILTHPGIPCIFYDHVFDWNLQHEIATLAEIRSRNGIHAESTLDILKAE 381

Query: 253  ADLYMASVNDGKVIVKIGPRFDIGGLAPTSERFRVAATGHDFCVWE 116
             D+Y+A + DGKVI K+GPR+D GG+ P+   F V A G+D+CVWE
Sbjct: 382  GDIYVAMI-DGKVITKLGPRYDAGGIIPSD--FHVVAHGNDYCVWE 424


>gb|AAN01149.1| alpha-amylase precursor [Musa acuminata]
          Length = 416

 Score =  534 bits (1375), Expect = e-149
 Identities = 244/410 (59%), Positives = 304/410 (74%)
 Frame = -2

Query: 1345 ALEAIMLQGFNWESWRTKPSWYSVLKGKAKEISETGVTDVWMPPPSHSVAPQGYMPGRLY 1166
            A   I+ QGFNWESWR +  WY+ LK K  +I+  GVT VW+PPPSHSV  QGYMPGRLY
Sbjct: 13   AQSQILFQGFNWESWRQQGGWYNFLKDKVSDIANAGVTHVWLPPPSHSVGVQGYMPGRLY 72

Query: 1165 DLDASAYGKEQELKDLIDHLHKTGVRCMADIVINHRCGDAQDSRGVWCVFEGGTPDDRLD 986
            DL AS YG + ELK LI   H  GV+C+ADIVINHRC D QD RG+WC+FEGGT D RLD
Sbjct: 73   DLGASKYGNQDELKALIGAFHDKGVKCVADIVINHRCADKQDGRGIWCIFEGGTDDARLD 132

Query: 985  WGPWAIVKDDHPYTDGRGNHDTGDDFGAAPDIDHTNPRVQNDISEWLAWLKCDVGFDGWR 806
            WGP  I +DD  Y+DG GN DTG+ F AAPDIDH N +VQ+++++WL WLK D+GFDGWR
Sbjct: 133  WGPHMICRDDTQYSDGTGNLDTGEGFAAAPDIDHLNTQVQHELTDWLNWLKTDIGFDGWR 192

Query: 805  FDFAKGYSPEYVRLYVERTFGDPRTSLCVGEIWNSLRYGHDGRPEYEQGQHREEILNWVR 626
             DFAKGYS    ++YVE+T    + +  V EIW+SL Y +DG+P Y+Q  +R+ ++NWV+
Sbjct: 193  LDFAKGYSSSIAKIYVEQT----QPNFVVAEIWSSLAYRNDGKPTYDQNGNRQGLVNWVQ 248

Query: 625  GTGGLCSAFDFTTKGILQEALGASELWRLRDSSMSPPGVIGAWPEKAVTFIDNHDTGSTQ 446
              GG  +AFDFTTKGILQ A+   ELWR+RD     PG++G WPEKAVTF+DNHDTGSTQ
Sbjct: 249  QVGGPVTAFDFTTKGILQAAV-EGELWRMRDPQGKAPGMMGWWPEKAVTFVDNHDTGSTQ 307

Query: 445  GHWAFPSDKVMLGYAYILLHPGVPCIFYDHLFDWGIGDGIKKLVDVRRRNGVKANSKCEI 266
              W FPSDKVM GYAYIL HPGVP IFYDH+FDWG+ + I +L   R RN + + S   I
Sbjct: 308  KLWPFPSDKVMQGYAYILTHPGVPSIFYDHMFDWGLKEKITRLAKTRTRNRIHSGSSLNI 367

Query: 265  LAAEADLYMASVNDGKVIVKIGPRFDIGGLAPTSERFRVAATGHDFCVWE 116
            LA++ADLYMA + DGK++ K+G R+D+G L P++  F V A+G+D+CVWE
Sbjct: 368  LASDADLYMAMI-DGKILTKLGSRYDVGNLVPSN--FHVVASGNDYCVWE 414


>ref|XP_006659493.1| PREDICTED: alpha-amylase isozyme 3E-like [Oryza brachyantha]
          Length = 436

 Score =  532 bits (1371), Expect = e-148
 Identities = 246/406 (60%), Positives = 300/406 (73%)
 Frame = -2

Query: 1333 IMLQGFNWESWRTKPSWYSVLKGKAKEISETGVTDVWMPPPSHSVAPQGYMPGRLYDLDA 1154
            ++ QGFNWESWR +  WY+ L  K +EI+ TGVT VW+PPPSHSV+PQGYMPGRLYDLDA
Sbjct: 27   VLFQGFNWESWRMQGGWYNFLHEKVEEIASTGVTHVWLPPPSHSVSPQGYMPGRLYDLDA 86

Query: 1153 SAYGKEQELKDLIDHLHKTGVRCMADIVINHRCGDAQDSRGVWCVFEGGTPDDRLDWGPW 974
            S YG E EL+ LI+  H+  V C+ADIVINHRC D +DSRGV+CVFEGGTPD RLDWGP 
Sbjct: 87   SKYGTEAELRSLIEAFHEKNVECLADIVINHRCADYKDSRGVYCVFEGGTPDGRLDWGPD 146

Query: 973  AIVKDDHPYTDGRGNHDTGDDFGAAPDIDHTNPRVQNDISEWLAWLKCDVGFDGWRFDFA 794
             I  DD  Y++GRG+ DTG  FGAAPDIDH NPRVQ ++++WL WLK D+GFDGWR DFA
Sbjct: 147  MICSDDTQYSNGRGHRDTGAGFGAAPDIDHLNPRVQRELTDWLNWLKADLGFDGWRLDFA 206

Query: 793  KGYSPEYVRLYVERTFGDPRTSLCVGEIWNSLRYGHDGRPEYEQGQHREEILNWVRGTGG 614
            KGYS    R+YV+ T      +  VGEIW+SL Y  DG+P   Q   R+E++NWV G G 
Sbjct: 207  KGYSAPIARVYVDNT----NPTFVVGEIWSSLIYNGDGKPSTNQDADRQELVNWVDGVGK 262

Query: 613  LCSAFDFTTKGILQEALGASELWRLRDSSMSPPGVIGAWPEKAVTFIDNHDTGSTQGHWA 434
              +AFDFTTKGILQ A+   ELWRL D +   PG++G  P++AVTF+DNHDTGSTQ  W 
Sbjct: 263  PATAFDFTTKGILQAAV-QGELWRLHDGNGKAPGLMGWMPDQAVTFVDNHDTGSTQSLWP 321

Query: 433  FPSDKVMLGYAYILLHPGVPCIFYDHLFDWGIGDGIKKLVDVRRRNGVKANSKCEILAAE 254
            FPSDKVM GYAYIL HPG+PCIFYDH+FDW +   I  L  +R RNG+   S  +IL AE
Sbjct: 322  FPSDKVMQGYAYILTHPGIPCIFYDHVFDWNLQHEIATLAAIRSRNGIHPESTLDILKAE 381

Query: 253  ADLYMASVNDGKVIVKIGPRFDIGGLAPTSERFRVAATGHDFCVWE 116
             D+Y+A + DG+VI K+GPR+D GG+ P+   F V A G+D+CVWE
Sbjct: 382  GDIYVAMI-DGRVITKLGPRYDAGGIIPSD--FHVVAHGNDYCVWE 424


>ref|XP_004957017.1| PREDICTED: alpha-amylase isozyme 3C-like [Setaria italica]
          Length = 437

 Score =  532 bits (1371), Expect = e-148
 Identities = 246/410 (60%), Positives = 303/410 (73%)
 Frame = -2

Query: 1345 ALEAIMLQGFNWESWRTKPSWYSVLKGKAKEISETGVTDVWMPPPSHSVAPQGYMPGRLY 1166
            A   ++ QGFNWESW+ +  WY+ L+G+  +I+ TG T VW+PPPSHSVAPQGYMPGRLY
Sbjct: 24   AQSQVLFQGFNWESWKKQGGWYNFLRGRVDDIAATGATHVWLPPPSHSVAPQGYMPGRLY 83

Query: 1165 DLDASAYGKEQELKDLIDHLHKTGVRCMADIVINHRCGDAQDSRGVWCVFEGGTPDDRLD 986
            DLDAS YG   ELK LI   H  G++C+ADIVINHRC D +DSRG++CVFEGGTPD RLD
Sbjct: 84   DLDASKYGTHAELKSLIAAFHAKGIQCVADIVINHRCADYKDSRGIYCVFEGGTPDSRLD 143

Query: 985  WGPWAIVKDDHPYTDGRGNHDTGDDFGAAPDIDHTNPRVQNDISEWLAWLKCDVGFDGWR 806
            WGP  I  DD  Y++GRG+ DTG DFGAAPDIDH NPRVQ ++S+WL WLK D+GFDGWR
Sbjct: 144  WGPDMICSDDTQYSNGRGHRDTGADFGAAPDIDHLNPRVQQELSDWLNWLKTDLGFDGWR 203

Query: 805  FDFAKGYSPEYVRLYVERTFGDPRTSLCVGEIWNSLRYGHDGRPEYEQGQHREEILNWVR 626
             DFAKGYS    ++YV+ T      +  V EIW+SL+Y  +G P   Q + R+E++NW +
Sbjct: 204  LDFAKGYSAAVAKVYVDNT----APTFVVAEIWSSLQYDGNGEPSSIQDKDRQELVNWAQ 259

Query: 625  GTGGLCSAFDFTTKGILQEALGASELWRLRDSSMSPPGVIGAWPEKAVTFIDNHDTGSTQ 446
              GG  +AFDFTTKG+LQ A+   ELWR++D +   PG+IG  PEKAVTF+DNHDTGSTQ
Sbjct: 260  AVGGPAAAFDFTTKGVLQAAV-QGELWRMKDGNGKAPGMIGWLPEKAVTFVDNHDTGSTQ 318

Query: 445  GHWAFPSDKVMLGYAYILLHPGVPCIFYDHLFDWGIGDGIKKLVDVRRRNGVKANSKCEI 266
              W FPSDKVM GYAYIL HPG PCIFYDH+FDW +   I  L  VR RNG+ A SK +I
Sbjct: 319  NSWPFPSDKVMQGYAYILTHPGTPCIFYDHVFDWNLKQEISTLSTVRSRNGIHAGSKLDI 378

Query: 265  LAAEADLYMASVNDGKVIVKIGPRFDIGGLAPTSERFRVAATGHDFCVWE 116
            LAA+ DLY+A ++D KVIVKIG R+D+G L P+   F   A G+++CVWE
Sbjct: 379  LAADGDLYVAKIDD-KVIVKIGSRYDVGNLIPSD--FHPIAHGNNYCVWE 425


>ref|XP_004957016.1| PREDICTED: alpha-amylase isozyme 3C-like [Setaria italica]
          Length = 437

 Score =  532 bits (1371), Expect = e-148
 Identities = 245/410 (59%), Positives = 304/410 (74%)
 Frame = -2

Query: 1345 ALEAIMLQGFNWESWRTKPSWYSVLKGKAKEISETGVTDVWMPPPSHSVAPQGYMPGRLY 1166
            A   ++ QGFNWESW+ +  WY+ L+G+  +I+ TG T VW+PPPSHSVAPQGYMPGRLY
Sbjct: 24   AQSQVLFQGFNWESWKKQGGWYNFLRGRVDDIAATGATHVWLPPPSHSVAPQGYMPGRLY 83

Query: 1165 DLDASAYGKEQELKDLIDHLHKTGVRCMADIVINHRCGDAQDSRGVWCVFEGGTPDDRLD 986
            DLDAS YG   ELK LI   H  G++C+ADIVINHRC D +DSRG++CVFEGGTPD RLD
Sbjct: 84   DLDASKYGTHAELKSLIAAFHAKGIQCVADIVINHRCADYKDSRGIYCVFEGGTPDSRLD 143

Query: 985  WGPWAIVKDDHPYTDGRGNHDTGDDFGAAPDIDHTNPRVQNDISEWLAWLKCDVGFDGWR 806
            WGP  I  DD  Y++GRG+ DTG DFGAAPDIDH NPRVQ ++++WL WLK D+GFDGWR
Sbjct: 144  WGPDMICSDDTQYSNGRGHRDTGADFGAAPDIDHLNPRVQQELADWLNWLKTDLGFDGWR 203

Query: 805  FDFAKGYSPEYVRLYVERTFGDPRTSLCVGEIWNSLRYGHDGRPEYEQGQHREEILNWVR 626
             DFAKGYS    ++YV+ T      +  V EIW+SL+Y  +G P   Q + R+E++NW +
Sbjct: 204  LDFAKGYSAAVAKVYVDNT----APTFVVAEIWSSLQYDGNGEPSSIQDKDRQELVNWAQ 259

Query: 625  GTGGLCSAFDFTTKGILQEALGASELWRLRDSSMSPPGVIGAWPEKAVTFIDNHDTGSTQ 446
              GG  +AFDFTTKG+LQ A+   ELWR++D +   PG+IG  PEKAVTF+DNHDTGSTQ
Sbjct: 260  AVGGPAAAFDFTTKGVLQAAV-QGELWRMKDGNGKAPGMIGWLPEKAVTFVDNHDTGSTQ 318

Query: 445  GHWAFPSDKVMLGYAYILLHPGVPCIFYDHLFDWGIGDGIKKLVDVRRRNGVKANSKCEI 266
              W FPSDKVM GYAYIL HPG PCIFYDH+FDW +   I  L  VR RNG+ A SK +I
Sbjct: 319  NSWPFPSDKVMQGYAYILTHPGTPCIFYDHVFDWNLKQEISTLSTVRSRNGIHAGSKLDI 378

Query: 265  LAAEADLYMASVNDGKVIVKIGPRFDIGGLAPTSERFRVAATGHDFCVWE 116
            LAA+ DLY+A ++D KVIVKIG R+D+G L P+   F + A G+++CVWE
Sbjct: 379  LAADGDLYVAKIDD-KVIVKIGSRYDVGNLIPSD--FHLIAHGNNYCVWE 425


>ref|XP_004957015.1| PREDICTED: alpha-amylase isozyme 3C-like [Setaria italica]
          Length = 437

 Score =  531 bits (1368), Expect = e-148
 Identities = 245/410 (59%), Positives = 303/410 (73%)
 Frame = -2

Query: 1345 ALEAIMLQGFNWESWRTKPSWYSVLKGKAKEISETGVTDVWMPPPSHSVAPQGYMPGRLY 1166
            A   ++ QGFNWESW+ +  WY+ L+G+  +I+ TG T VW+PPPSHSVAPQGYMPGRLY
Sbjct: 24   AQSQVLFQGFNWESWKKQGGWYNFLRGRVDDIAATGATHVWLPPPSHSVAPQGYMPGRLY 83

Query: 1165 DLDASAYGKEQELKDLIDHLHKTGVRCMADIVINHRCGDAQDSRGVWCVFEGGTPDDRLD 986
            DLDAS YG   ELK LI   H  G++C+ADIVINHRC D +DSRG++CVFEGGTPD RLD
Sbjct: 84   DLDASKYGTHAELKSLIAAFHAKGIQCVADIVINHRCADYKDSRGIYCVFEGGTPDSRLD 143

Query: 985  WGPWAIVKDDHPYTDGRGNHDTGDDFGAAPDIDHTNPRVQNDISEWLAWLKCDVGFDGWR 806
            WGP  I  DD  Y++GRG+ DTG DFGAAPDIDH NPRVQ ++++WL WLK D+GFDGWR
Sbjct: 144  WGPDMICSDDTQYSNGRGHRDTGADFGAAPDIDHLNPRVQQELADWLNWLKTDLGFDGWR 203

Query: 805  FDFAKGYSPEYVRLYVERTFGDPRTSLCVGEIWNSLRYGHDGRPEYEQGQHREEILNWVR 626
             DFAKGYS    ++YV+ T      +  V EIW+SL+Y  +G P   Q + R+E++NW +
Sbjct: 204  LDFAKGYSAAVAKVYVDNT----APTFVVAEIWSSLQYDGNGEPSSIQDKDRQELVNWAQ 259

Query: 625  GTGGLCSAFDFTTKGILQEALGASELWRLRDSSMSPPGVIGAWPEKAVTFIDNHDTGSTQ 446
              GG  +AFDFTTKG+LQ A+   ELWR++D +   PG+IG  PEKAVTF+DNHDTGSTQ
Sbjct: 260  AVGGPAAAFDFTTKGVLQAAV-QGELWRMKDGNGKAPGMIGWLPEKAVTFVDNHDTGSTQ 318

Query: 445  GHWAFPSDKVMLGYAYILLHPGVPCIFYDHLFDWGIGDGIKKLVDVRRRNGVKANSKCEI 266
              W FPSDKVM GYAYIL HPG PCIFYDH+FDW +   I  L  VR RNG+ A SK +I
Sbjct: 319  NSWPFPSDKVMQGYAYILTHPGTPCIFYDHVFDWNLKQEISTLSTVRSRNGIHAGSKLDI 378

Query: 265  LAAEADLYMASVNDGKVIVKIGPRFDIGGLAPTSERFRVAATGHDFCVWE 116
            LAA+ DLY+A ++D KVIVKIG R+D+G L P+   F   A G+++CVWE
Sbjct: 379  LAADGDLYVAKIDD-KVIVKIGSRYDVGNLIPSD--FHPIAHGNNYCVWE 425


>gb|AAA98615.1| alpha-amylase [Hordeum vulgare subsp. vulgare]
          Length = 427

 Score =  531 bits (1368), Expect = e-148
 Identities = 247/408 (60%), Positives = 299/408 (73%), Gaps = 2/408 (0%)
 Frame = -2

Query: 1333 IMLQGFNWESWRTKPSWYSVLKGKAKEISETGVTDVWMPPPSHSVAPQGYMPGRLYDLDA 1154
            ++ QGFNWESW+    WY+ L GK  +I+  GVT VW+PP S SVA QGYMPGRLYDLDA
Sbjct: 26   VLFQGFNWESWKHNGGWYNFLMGKVDDIAAAGVTHVWLPPASQSVAEQGYMPGRLYDLDA 85

Query: 1153 SAYGKEQELKDLIDHLHKTGVRCMADIVINHRCGDAQDSRGVWCVFEGGTPDDRLDWGPW 974
            S YG + +LK LI  LH  GV+ +ADIVINHR  + +D RG++C+FEGGTPD RLDWGP 
Sbjct: 86   SKYGNKAQLKSLIGALHGKGVKAIADIVINHRTAERKDGRGIYCIFEGGTPDARLDWGPH 145

Query: 973  AIVKDDHPYTDGRGNHDTGDDFGAAPDIDHTNPRVQNDISEWLAWLKCDVGFDGWRFDFA 794
             I +DD PY DG GN DTG DFGAAPDIDH NPRVQ ++ EWL WL+ DVGFDGWRFDFA
Sbjct: 146  MICRDDRPYADGTGNPDTGADFGAAPDIDHLNPRVQKELVEWLNWLRTDVGFDGWRFDFA 205

Query: 793  KGYSPEYVRLYVERTFGDPRTSLCVGEIWNSLRYGHDGRPEYEQGQHREEILNWVRGTGG 614
            KGYS +  ++YV+R+      S  V EIW SL YG DG+P   Q  HR+E++NWV   GG
Sbjct: 206  KGYSADVAKIYVDRS----EPSFAVAEIWTSLAYGGDGKPNLNQDPHRQELVNWVNKVGG 261

Query: 613  L--CSAFDFTTKGILQEALGASELWRLRDSSMSPPGVIGAWPEKAVTFIDNHDTGSTQGH 440
                + FDFTTKGIL  A+   ELWRLR +    PG+IG WP KAVTF+DNHDTGSTQ  
Sbjct: 262  SGPATTFDFTTKGILNVAV-EGELWRLRGTDGKAPGMIGWWPAKAVTFVDNHDTGSTQHM 320

Query: 439  WAFPSDKVMLGYAYILLHPGVPCIFYDHLFDWGIGDGIKKLVDVRRRNGVKANSKCEILA 260
            W FPSD+VM GYAYIL HPG PCIFYDH FDWG+ + I +LV +R R G+ + SK +I+ 
Sbjct: 321  WPFPSDRVMQGYAYILTHPGTPCIFYDHFFDWGLKEEIDRLVSIRTRQGIHSESKLQIME 380

Query: 259  AEADLYMASVNDGKVIVKIGPRFDIGGLAPTSERFRVAATGHDFCVWE 116
            A+ADLY+A + DGKVIVK+GPR+D+G L P  E F+V A G+D+ VWE
Sbjct: 381  ADADLYLAEI-DGKVIVKLGPRYDVGHLIP--EGFKVVAHGNDYAVWE 425


>emb|CAX51374.1| alpha-amylase [Hordeum vulgare subsp. vulgare]
            gi|326515380|dbj|BAK03603.1| predicted protein [Hordeum
            vulgare subsp. vulgare]
          Length = 427

 Score =  531 bits (1367), Expect = e-148
 Identities = 252/437 (57%), Positives = 310/437 (70%), Gaps = 2/437 (0%)
 Frame = -2

Query: 1414 VNATLASGQPGLKMSRGGNIQTDALEAIMLQGFNWESWRTKPSWYSVLKGKAKEISETGV 1235
            ++A+LASGQ                  ++ QGFNWESW+    WY+ L GK  +I+  G+
Sbjct: 17   LSASLASGQ------------------VLFQGFNWESWKHNGGWYNFLMGKVDDIAAAGI 58

Query: 1234 TDVWMPPPSHSVAPQGYMPGRLYDLDASAYGKEQELKDLIDHLHKTGVRCMADIVINHRC 1055
            T VW+PP S SVA QGYMPGRLYDLDAS YG + +LK LI  LH  GV+ +ADIVINHR 
Sbjct: 59   THVWLPPASQSVAEQGYMPGRLYDLDASKYGNKAQLKSLIGALHGKGVKAIADIVINHRT 118

Query: 1054 GDAQDSRGVWCVFEGGTPDDRLDWGPWAIVKDDHPYTDGRGNHDTGDDFGAAPDIDHTNP 875
             + +D RG++C+FEGGTPD RLDWGP  I +DD PY DG GN DTG DFGAAPDIDH N 
Sbjct: 119  AEHKDGRGIYCIFEGGTPDARLDWGPHMICRDDRPYADGTGNPDTGADFGAAPDIDHLNL 178

Query: 874  RVQNDISEWLAWLKCDVGFDGWRFDFAKGYSPEYVRLYVERTFGDPRTSLCVGEIWNSLR 695
            RVQ ++ EWL WLK D+GFDGWRFDFAKGYS +  ++Y++R+      S  V EIW SL 
Sbjct: 179  RVQKELVEWLNWLKADIGFDGWRFDFAKGYSADVAKIYIDRS----EPSFAVAEIWTSLA 234

Query: 694  YGHDGRPEYEQGQHREEILNWVRGTGGL--CSAFDFTTKGILQEALGASELWRLRDSSMS 521
            YG DG+P   Q QHR+E++NWV   GG    + FDFTTKGIL  A+   ELWRLR +   
Sbjct: 235  YGGDGKPNLNQDQHRQELVNWVDKVGGKGPATTFDFTTKGILNVAV-EGELWRLRGTDGK 293

Query: 520  PPGVIGAWPEKAVTFIDNHDTGSTQGHWAFPSDKVMLGYAYILLHPGVPCIFYDHLFDWG 341
             PG+IG WP KAVTF+DNHDTGSTQ  W FPSD+VM GYAYIL HPG PCIFYDH FDWG
Sbjct: 294  APGMIGWWPAKAVTFVDNHDTGSTQHMWPFPSDRVMQGYAYILTHPGTPCIFYDHFFDWG 353

Query: 340  IGDGIKKLVDVRRRNGVKANSKCEILAAEADLYMASVNDGKVIVKIGPRFDIGGLAPTSE 161
            + + I +LV VR R+G+ + SK +I+ A+ADLY+A + DGKVIVK+GPR+D+G L P   
Sbjct: 354  LKEEIDRLVSVRTRHGIHSESKLQIIEADADLYLAEI-DGKVIVKLGPRYDVGNLIPGG- 411

Query: 160  RFRVAATGHDFCVWEAL 110
             F+VAA G+D+ VWE +
Sbjct: 412  -FKVAAHGNDYAVWEKI 427


>emb|CAX51373.1| alpha-amylase [Hordeum vulgare subsp. vulgare]
          Length = 427

 Score =  530 bits (1365), Expect = e-148
 Identities = 247/408 (60%), Positives = 300/408 (73%), Gaps = 2/408 (0%)
 Frame = -2

Query: 1333 IMLQGFNWESWRTKPSWYSVLKGKAKEISETGVTDVWMPPPSHSVAPQGYMPGRLYDLDA 1154
            ++ QGFNWESW+    WY+ L GK  +I+  GVT VW+PP S SVA QGYMPGRLYDLDA
Sbjct: 26   VLFQGFNWESWKHNGGWYNFLMGKVDDIAAAGVTHVWLPPASQSVAEQGYMPGRLYDLDA 85

Query: 1153 SAYGKEQELKDLIDHLHKTGVRCMADIVINHRCGDAQDSRGVWCVFEGGTPDDRLDWGPW 974
            S YG + +LK LI  LH  GV+ +ADIVINHR  + +D RG++C+FEGGTPD RLDWGP 
Sbjct: 86   SKYGNKAQLKSLIGALHGKGVKAIADIVINHRTAERKDGRGIYCIFEGGTPDARLDWGPH 145

Query: 973  AIVKDDHPYTDGRGNHDTGDDFGAAPDIDHTNPRVQNDISEWLAWLKCDVGFDGWRFDFA 794
             I +DD PY DG GN DTG DFGAAPDIDH NPRVQ ++ EWL WL+ DVGFDGWRFDFA
Sbjct: 146  MICRDDRPYADGTGNPDTGADFGAAPDIDHLNPRVQKELVEWLNWLRTDVGFDGWRFDFA 205

Query: 793  KGYSPEYVRLYVERTFGDPRTSLCVGEIWNSLRYGHDGRPEYEQGQHREEILNWVRGTGG 614
            KGYS +  ++YV+R+      S  V EIW SL YG DG+P   Q  HR+E++NWV   GG
Sbjct: 206  KGYSADVAKIYVDRS----EPSFAVAEIWTSLAYGGDGKPNLNQDPHRQELVNWVNKVGG 261

Query: 613  L--CSAFDFTTKGILQEALGASELWRLRDSSMSPPGVIGAWPEKAVTFIDNHDTGSTQGH 440
                + FDFTTKGIL  A+   ELWRLR +    PG+IG WP KAVTF+DNHDTGSTQ  
Sbjct: 262  SGPATTFDFTTKGILNVAV-EGELWRLRGTDGKAPGMIGWWPAKAVTFVDNHDTGSTQHM 320

Query: 439  WAFPSDKVMLGYAYILLHPGVPCIFYDHLFDWGIGDGIKKLVDVRRRNGVKANSKCEILA 260
            W FPSD+VM GYAYIL HPG PCIFYDH FDWG+ + I +LV +R R+G+ + SK +I+ 
Sbjct: 321  WPFPSDRVMQGYAYILTHPGTPCIFYDHFFDWGLKEEIDRLVSIRTRHGIHSESKLQIME 380

Query: 259  AEADLYMASVNDGKVIVKIGPRFDIGGLAPTSERFRVAATGHDFCVWE 116
            A+ADLY+A + DGKVIVK+GPR+D+  L P  E F+VAA G+D+ VWE
Sbjct: 381  ADADLYLAEI-DGKVIVKLGPRYDVRHLIP--EGFKVAAHGNDYAVWE 425


>sp|P04063.3|AMY2_HORVU RecName: Full=Alpha-amylase type B isozyme; AltName:
            Full=1,4-alpha-D-glucan glucanohydrolase; AltName:
            Full=AMY2-2; AltName: Full=High pI alpha-amylase; Flags:
            Precursor gi|166995|gb|AAA98790.1| alpha-amylase type B
            isozyme precursor [Hordeum vulgare subsp. vulgare]
          Length = 427

 Score =  530 bits (1364), Expect = e-148
 Identities = 252/437 (57%), Positives = 309/437 (70%), Gaps = 2/437 (0%)
 Frame = -2

Query: 1414 VNATLASGQPGLKMSRGGNIQTDALEAIMLQGFNWESWRTKPSWYSVLKGKAKEISETGV 1235
            ++A+LASGQ                  ++ QGFNWESW+    WY+ L GK  +I+  G+
Sbjct: 17   LSASLASGQ------------------VLFQGFNWESWKHNGGWYNFLMGKVDDIAAAGI 58

Query: 1234 TDVWMPPPSHSVAPQGYMPGRLYDLDASAYGKEQELKDLIDHLHKTGVRCMADIVINHRC 1055
            T VW+PP S SVA QGYMPGRLYDLDAS YG + +LK LI  LH  GV+ +ADIVINHR 
Sbjct: 59   THVWLPPASQSVAEQGYMPGRLYDLDASKYGNKAQLKSLIGALHGKGVKAIADIVINHRT 118

Query: 1054 GDAQDSRGVWCVFEGGTPDDRLDWGPWAIVKDDHPYTDGRGNHDTGDDFGAAPDIDHTNP 875
             + +D RG++C+FEGGTPD RLDWGP  I +DD PY DG GN DTG DFGAAPDIDH N 
Sbjct: 119  AEHKDGRGIYCIFEGGTPDARLDWGPHMICRDDRPYADGTGNPDTGADFGAAPDIDHLNL 178

Query: 874  RVQNDISEWLAWLKCDVGFDGWRFDFAKGYSPEYVRLYVERTFGDPRTSLCVGEIWNSLR 695
            RVQ ++ EWL WLK D+GFDGWRFDFAKGYS +  ++Y++R+      S  V EIW SL 
Sbjct: 179  RVQKELVEWLNWLKADIGFDGWRFDFAKGYSADVAKIYIDRS----EPSFAVAEIWTSLA 234

Query: 694  YGHDGRPEYEQGQHREEILNWVRGTGGL--CSAFDFTTKGILQEALGASELWRLRDSSMS 521
            YG DG+P   Q QHR+E++NWV   GG    + FDFTTKGIL  A+   ELWRLR +   
Sbjct: 235  YGGDGKPNLNQDQHRQELVNWVDKVGGKGPATTFDFTTKGILNVAV-EGELWRLRGTDGK 293

Query: 520  PPGVIGAWPEKAVTFIDNHDTGSTQGHWAFPSDKVMLGYAYILLHPGVPCIFYDHLFDWG 341
             PG+IG WP KAVTF+DNHDTGSTQ  W FPSD+VM GYAYIL HPG PCIFYDH FDWG
Sbjct: 294  APGMIGWWPAKAVTFVDNHDTGSTQHMWPFPSDRVMQGYAYILTHPGTPCIFYDHFFDWG 353

Query: 340  IGDGIKKLVDVRRRNGVKANSKCEILAAEADLYMASVNDGKVIVKIGPRFDIGGLAPTSE 161
            + + I +LV VR R+G+   SK +I+ A+ADLY+A + DGKVIVK+GPR+D+G L P   
Sbjct: 354  LKEEIDRLVSVRTRHGIHNESKLQIIEADADLYLAEI-DGKVIVKLGPRYDVGNLIPGG- 411

Query: 160  RFRVAATGHDFCVWEAL 110
             F+VAA G+D+ VWE +
Sbjct: 412  -FKVAAHGNDYAVWEKI 427


>gb|EMT32633.1| Alpha-amylase type B isozyme [Aegilops tauschii]
          Length = 427

 Score =  529 bits (1363), Expect = e-147
 Identities = 246/410 (60%), Positives = 298/410 (72%), Gaps = 2/410 (0%)
 Frame = -2

Query: 1333 IMLQGFNWESWRTKPSWYSVLKGKAKEISETGVTDVWMPPPSHSVAPQGYMPGRLYDLDA 1154
            ++ QGFNWESW+    WY+ L GK  +I+  GVT VW+PP S SV+ QGYMPGRLYDLDA
Sbjct: 26   VLFQGFNWESWKHNGGWYNFLMGKVDDIAAAGVTHVWLPPASQSVSKQGYMPGRLYDLDA 85

Query: 1153 SAYGKEQELKDLIDHLHKTGVRCMADIVINHRCGDAQDSRGVWCVFEGGTPDDRLDWGPW 974
            S YG + +LK LI  LH  GV+ +ADIVINHR  + +D RG++C+FEGGTPD RLDWGP 
Sbjct: 86   SKYGNKAQLKSLIGALHGKGVKAIADIVINHRTAERKDGRGIYCIFEGGTPDARLDWGPH 145

Query: 973  AIVKDDHPYTDGRGNHDTGDDFGAAPDIDHTNPRVQNDISEWLAWLKCDVGFDGWRFDFA 794
             I +DD PY DG GN DTG DFGAAPDIDH NPRVQ ++ EWL WL+ DVGFDGWRFDFA
Sbjct: 146  MICRDDRPYADGTGNPDTGADFGAAPDIDHLNPRVQKELVEWLNWLRTDVGFDGWRFDFA 205

Query: 793  KGYSPEYVRLYVERTFGDPRTSLCVGEIWNSLRYGHDGRPEYEQGQHREEILNWVR--GT 620
            KGYS +  ++Y++R+      S  V EIW SL YG DG+P   Q  HR+E++NWV   G 
Sbjct: 206  KGYSADVAKIYIDRS----EASFAVAEIWTSLAYGGDGKPNLNQDPHRQELVNWVNKVGG 261

Query: 619  GGLCSAFDFTTKGILQEALGASELWRLRDSSMSPPGVIGAWPEKAVTFIDNHDTGSTQGH 440
             G  + FDFTTKGIL  A+   ELWRLR +    PG+IG WP KAVTF+DNHDTGSTQ  
Sbjct: 262  SGTGTTFDFTTKGILNVAV-EGELWRLRGTDGKAPGMIGWWPAKAVTFVDNHDTGSTQHM 320

Query: 439  WAFPSDKVMLGYAYILLHPGVPCIFYDHLFDWGIGDGIKKLVDVRRRNGVKANSKCEILA 260
            W FPSD VM GYAYIL HPG PCIFYDH FDWG+ D I +LV +R R G+ + SK +I+ 
Sbjct: 321  WPFPSDSVMQGYAYILTHPGTPCIFYDHFFDWGMKDEIYRLVSIRTRQGIHSESKLQIME 380

Query: 259  AEADLYMASVNDGKVIVKIGPRFDIGGLAPTSERFRVAATGHDFCVWEAL 110
            A+ADLY+A + DGK+IVK+GPRFD+G L P    F+VAA G D+ VWE +
Sbjct: 381  ADADLYLAEI-DGKIIVKLGPRFDVGHLIPGG--FKVAAHGKDYAVWEKI 427


>pdb|1AVA|A Chain A, Amy2BASI PROTEIN-Protein Complex From Barley Seed
            gi|4699832|pdb|1AVA|B Chain B, Amy2BASI PROTEIN-Protein
            Complex From Barley Seed gi|157829976|pdb|1AMY|A Chain A,
            Crystal And Molecular Structure Of Barley Alpha-Amylase
            gi|157830292|pdb|1BG9|A Chain A, Barley Alpha-Amylase
            With Substrate Analogue Acarbose
          Length = 403

 Score =  529 bits (1363), Expect = e-147
 Identities = 246/410 (60%), Positives = 300/410 (73%), Gaps = 2/410 (0%)
 Frame = -2

Query: 1333 IMLQGFNWESWRTKPSWYSVLKGKAKEISETGVTDVWMPPPSHSVAPQGYMPGRLYDLDA 1154
            ++ QGFNWESW+    WY+ L GK  +I+  G+T VW+PP S SVA QGYMPGRLYDLDA
Sbjct: 2    VLFQGFNWESWKHNGGWYNFLMGKVDDIAAAGITHVWLPPASQSVAEQGYMPGRLYDLDA 61

Query: 1153 SAYGKEQELKDLIDHLHKTGVRCMADIVINHRCGDAQDSRGVWCVFEGGTPDDRLDWGPW 974
            S YG + +LK LI  LH  GV+ +ADIVINHR  + +D RG++C+FEGGTPD RLDWGP 
Sbjct: 62   SKYGNKAQLKSLIGALHGKGVKAIADIVINHRTAEHKDGRGIYCIFEGGTPDARLDWGPH 121

Query: 973  AIVKDDHPYTDGRGNHDTGDDFGAAPDIDHTNPRVQNDISEWLAWLKCDVGFDGWRFDFA 794
             I +DD PY DG GN DTG DFGAAPDIDH N RVQ ++ EWL WLK D+GFDGWRFDFA
Sbjct: 122  MICRDDRPYADGTGNPDTGADFGAAPDIDHLNLRVQKELVEWLNWLKADIGFDGWRFDFA 181

Query: 793  KGYSPEYVRLYVERTFGDPRTSLCVGEIWNSLRYGHDGRPEYEQGQHREEILNWVRGTGG 614
            KGYS +  ++Y++R+      S  V EIW SL YG DG+P   Q QHR+E++NWV   GG
Sbjct: 182  KGYSADVAKIYIDRS----EPSFAVAEIWTSLAYGGDGKPNLNQDQHRQELVNWVDKVGG 237

Query: 613  L--CSAFDFTTKGILQEALGASELWRLRDSSMSPPGVIGAWPEKAVTFIDNHDTGSTQGH 440
                + FDFTTKGIL  A+   ELWRLR +    PG+IG WP KAVTF+DNHDTGSTQ  
Sbjct: 238  KGPATTFDFTTKGILNVAV-EGELWRLRGTDGKAPGMIGWWPAKAVTFVDNHDTGSTQHM 296

Query: 439  WAFPSDKVMLGYAYILLHPGVPCIFYDHLFDWGIGDGIKKLVDVRRRNGVKANSKCEILA 260
            W FPSD+VM GYAYIL HPG PCIFYDH FDWG+ + I +LV VR R+G+   SK +I+ 
Sbjct: 297  WPFPSDRVMQGYAYILTHPGTPCIFYDHFFDWGLKEEIDRLVSVRTRHGIHNESKLQIIE 356

Query: 259  AEADLYMASVNDGKVIVKIGPRFDIGGLAPTSERFRVAATGHDFCVWEAL 110
            A+ADLY+A + DGKVIVK+GPR+D+G L P    F+VAA G+D+ VWE +
Sbjct: 357  ADADLYLAEI-DGKVIVKLGPRYDVGNLIPGG--FKVAAHGNDYAVWEKI 403