BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Ephedra27_contig00019059
         (1666 letters)

Database: ./nr 
           37,332,560 sequences; 13,225,080,153 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_006470603.1| PREDICTED: mitochondrial tRNA-specific 2-thi...   463   e-127
emb|CBI16383.3| unnamed protein product [Vitis vinifera]              461   e-127
ref|XP_002282827.1| PREDICTED: tRNA-specific 2-thiouridylase mnm...   461   e-127
ref|XP_006358437.1| PREDICTED: mitochondrial tRNA-specific 2-thi...   460   e-127
ref|XP_002298404.2| hypothetical protein POPTR_0001s26620g [Popu...   460   e-127
ref|XP_002513510.1| tRNA (5-methylaminomethyl-2-thiouridylate)-m...   459   e-126
gb|EXB93298.1| tRNA-specific 2-thiouridylase mnmA [Morus notabilis]   458   e-126
ref|XP_006850208.1| hypothetical protein AMTR_s00022p00251260 [A...   458   e-126
ref|XP_004142116.1| PREDICTED: tRNA-specific 2-thiouridylase Mnm...   458   e-126
ref|XP_006307321.1| hypothetical protein CARUB_v10008942mg [Caps...   457   e-126
ref|XP_004247484.1| PREDICTED: tRNA-specific 2-thiouridylase Mnm...   457   e-126
ref|XP_006280360.1| hypothetical protein CARUB_v10026287mg [Caps...   457   e-126
ref|XP_004507093.1| PREDICTED: tRNA-specific 2-thiouridylase Mnm...   453   e-124
ref|XP_004301461.1| PREDICTED: tRNA-specific 2-thiouridylase Mnm...   453   e-124
ref|XP_006393065.1| hypothetical protein EUTSA_v10011432mg [Eutr...   451   e-124
dbj|BAD95081.1| hypothetical protein [Arabidopsis thaliana]           451   e-124
ref|XP_002894309.1| tRNA (5-methylaminomethyl-2-thiouridylate)-m...   450   e-124
ref|NP_175542.2| tRNA (5-methylaminomethyl-2-thiouridylate)-meth...   449   e-123
ref|XP_003539065.2| PREDICTED: mitochondrial tRNA-specific 2-thi...   449   e-123
gb|ESW04011.1| hypothetical protein PHAVU_011G059600g [Phaseolus...   447   e-123

>ref|XP_006470603.1| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1-like [Citrus
            sinensis]
          Length = 525

 Score =  463 bits (1191), Expect = e-127
 Identities = 237/436 (54%), Positives = 287/436 (65%), Gaps = 3/436 (0%)
 Frame = -1

Query: 1306 DEKYLQCCWPGGVKRPLKIXXXXXXXXXXXXXXXXXXXXGHHCTAFYLKIDQDYVYENYM 1127
            + +YL C  P   ++ LK+                    GH CTAFYLKI     +EN+ 
Sbjct: 56   EPRYLSCSMPH--QKGLKVAVLLSGGVDSSVALRLLHSAGHSCTAFYLKIWFQEDFENFW 113

Query: 1126 PGCPWEDDLKYVRSVCEQAGVDLQRIDFTDRYWKSVVSYMISEYKRGFTPNPDILCNTRI 947
              CPWE+D+KY ++VC+Q  V L+ +  TD YWK+VVSY+I EY+ G TPNPD+LCNTRI
Sbjct: 114  TECPWEEDMKYAKAVCDQVDVPLEVVHLTDEYWKNVVSYIIEEYRCGRTPNPDVLCNTRI 173

Query: 946  KFGEFLDYIDTMNFDYIASGHYARVQHSGTTSKDTSAILCLSLDNVKDQTYFXXXXXXXX 767
            KFG F+D I +M FDY+ASGHYA+V HS    +   ++L LS D VKDQTYF        
Sbjct: 174  KFGAFMDAISSMEFDYVASGHYAKVVHSSADQEYKPSVLELSKDKVKDQTYFLSHLSQAQ 233

Query: 766  XXXLIFPLGCIPKNEVRELAADMGLPNKDRKDSQGICFLGKVKFSEFVARHXXXXXXXXX 587
               LIFPLGCI K EVR+ A    LPNKDRKDSQGICFLGK+KFSEFVARH         
Sbjct: 234  LKRLIFPLGCISKEEVRKFATQFDLPNKDRKDSQGICFLGKIKFSEFVARHIGEMEGVIL 293

Query: 586  EAETGAFLGNHRGFWFYTIGQRQGLGLTGGPWYVVAKDVENNVIFVSRNYYSLEMKRSFF 407
            EAETG FLG H GFWFYTIGQRQGL L GGPWYVV KDV+NNV+FVSRNYYS + KR  F
Sbjct: 294  EAETGDFLGKHWGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYYSFDKKRRLF 353

Query: 406  HVSNIRWIYA-TPEDFENIKCKVRHGPGFYDCK--IEHAKDIYWKEHNLPHPVEDSTQEV 236
             V +++W+    PE+   ++CKVRHGPGFY+C   IEH              VED  + +
Sbjct: 354  RVGSLKWLSGLPPEEISRLQCKVRHGPGFYNCNLMIEH--------------VEDGGENI 399

Query: 235  YTVTLSENDQGLAAGQFAAFYKDNICLGSGIIVGSSKDAYFPVCSKALEIAKIKDKSVLG 56
              V LSE+DQGLAAGQFAAFY+   C+GSG+I+ S  D  FPVC KALEIA+++DKS LG
Sbjct: 400  AVVQLSEDDQGLAAGQFAAFYQGTRCIGSGVILESWDDQGFPVCEKALEIARMEDKSKLG 459

Query: 55   KPIKIGTPVKSTETKN 8
            KPIKI    ++ E  N
Sbjct: 460  KPIKIKVKPETPEEFN 475


>emb|CBI16383.3| unnamed protein product [Vitis vinifera]
          Length = 544

 Score =  461 bits (1187), Expect = e-127
 Identities = 238/434 (54%), Positives = 284/434 (65%), Gaps = 5/434 (1%)
 Frame = -1

Query: 1303 EKYLQCCWPGGVKRPLKIXXXXXXXXXXXXXXXXXXXXGHHCTAFYLKIDQDYVYENYMP 1124
            + YL C  P      LKI                    GH CTAFYLKI     +EN+  
Sbjct: 76   QPYLSCSLPNN---RLKIAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWS 132

Query: 1123 GCPWEDDLKYVRSVCEQAGVDLQRIDFTDRYWKSVVSYMISEYKRGFTPNPDILCNTRIK 944
             CPWEDDLKY ++VC+Q  V L+ +  TD YWK+VVSY+I EY+ G TPNPD+LCNTRIK
Sbjct: 133  ECPWEDDLKYAKAVCDQVDVPLEVVHLTDEYWKNVVSYIIEEYRCGRTPNPDVLCNTRIK 192

Query: 943  FGEFLDYIDTMNFDYIASGHYARVQHSGTTSKDTSAILCLSLDNVKDQTYFXXXXXXXXX 764
            FG F+D I +  FDY+ASGHYA V HS T   D  ++L LS D VKDQTYF         
Sbjct: 193  FGAFMDAISSKEFDYVASGHYANVVHSFTDQMDKPSVLELSKDMVKDQTYFLSHLSQIQL 252

Query: 763  XXLIFPLGCIPKNEVRELAADMGLPNKDRKDSQGICFLGKVKFSEFVARHXXXXXXXXXE 584
              LIFPLGCIPK+EVR LA  + LPN+DRKDSQGICFLGK+KFSEFVARH         E
Sbjct: 253  KRLIFPLGCIPKDEVRSLATKLDLPNRDRKDSQGICFLGKIKFSEFVARHIGEKEGIILE 312

Query: 583  AETGAFLGNHRGFWFYTIGQRQGLGLTGGPWYVVAKDVENNVIFVSRNYYSLEMKRSFFH 404
            AETG  LG HRGFWFYTIGQRQGL L GGPWYVV KDV+NNV+FVSRNY+SL+ +R  F 
Sbjct: 313  AETGDLLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSLDKRRRLFR 372

Query: 403  VSNIRWIYATPE-DFENIKCKVRHGPGFYDCKIEHAKDIYWKEHNLPHPVEDSTQEVYTV 227
            V +++WI   P      ++CKVRHGPGFY+C                 P ED  ++V  V
Sbjct: 373  VGSLKWISREPPGQINQLQCKVRHGPGFYNCSF------------TMEPGEDGHEDVAVV 420

Query: 226  TLSENDQGLAAGQFAAFYKDNICLGSGIIVGSSKDAYFPVCSKALEIAKIKDKSVLGKPI 47
             LSE+DQGLAAGQF AFY+   C+GSG+I+ S  D  FPVC+KALEIA+++DKS LGKP+
Sbjct: 421  QLSEDDQGLAAGQFTAFYQGRSCIGSGVILESWDDQGFPVCAKALEIARMEDKSKLGKPV 480

Query: 46   KI----GTPVKSTE 17
            KI     TP K ++
Sbjct: 481  KIKVRPETPPKGSD 494


>ref|XP_002282827.1| PREDICTED: tRNA-specific 2-thiouridylase mnmA-like [Vitis vinifera]
          Length = 540

 Score =  461 bits (1187), Expect = e-127
 Identities = 238/434 (54%), Positives = 284/434 (65%), Gaps = 5/434 (1%)
 Frame = -1

Query: 1303 EKYLQCCWPGGVKRPLKIXXXXXXXXXXXXXXXXXXXXGHHCTAFYLKIDQDYVYENYMP 1124
            + YL C  P      LKI                    GH CTAFYLKI     +EN+  
Sbjct: 72   QPYLSCSLPNN---RLKIAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWS 128

Query: 1123 GCPWEDDLKYVRSVCEQAGVDLQRIDFTDRYWKSVVSYMISEYKRGFTPNPDILCNTRIK 944
             CPWEDDLKY ++VC+Q  V L+ +  TD YWK+VVSY+I EY+ G TPNPD+LCNTRIK
Sbjct: 129  ECPWEDDLKYAKAVCDQVDVPLEVVHLTDEYWKNVVSYIIEEYRCGRTPNPDVLCNTRIK 188

Query: 943  FGEFLDYIDTMNFDYIASGHYARVQHSGTTSKDTSAILCLSLDNVKDQTYFXXXXXXXXX 764
            FG F+D I +  FDY+ASGHYA V HS T   D  ++L LS D VKDQTYF         
Sbjct: 189  FGAFMDAISSKEFDYVASGHYANVVHSFTDQMDKPSVLELSKDMVKDQTYFLSHLSQIQL 248

Query: 763  XXLIFPLGCIPKNEVRELAADMGLPNKDRKDSQGICFLGKVKFSEFVARHXXXXXXXXXE 584
              LIFPLGCIPK+EVR LA  + LPN+DRKDSQGICFLGK+KFSEFVARH         E
Sbjct: 249  KRLIFPLGCIPKDEVRSLATKLDLPNRDRKDSQGICFLGKIKFSEFVARHIGEKEGIILE 308

Query: 583  AETGAFLGNHRGFWFYTIGQRQGLGLTGGPWYVVAKDVENNVIFVSRNYYSLEMKRSFFH 404
            AETG  LG HRGFWFYTIGQRQGL L GGPWYVV KDV+NNV+FVSRNY+SL+ +R  F 
Sbjct: 309  AETGDLLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSLDKRRRLFR 368

Query: 403  VSNIRWIYATPE-DFENIKCKVRHGPGFYDCKIEHAKDIYWKEHNLPHPVEDSTQEVYTV 227
            V +++WI   P      ++CKVRHGPGFY+C                 P ED  ++V  V
Sbjct: 369  VGSLKWISREPPGQINQLQCKVRHGPGFYNCSF------------TMEPGEDGHEDVAVV 416

Query: 226  TLSENDQGLAAGQFAAFYKDNICLGSGIIVGSSKDAYFPVCSKALEIAKIKDKSVLGKPI 47
             LSE+DQGLAAGQF AFY+   C+GSG+I+ S  D  FPVC+KALEIA+++DKS LGKP+
Sbjct: 417  QLSEDDQGLAAGQFTAFYQGRSCIGSGVILESWDDQGFPVCAKALEIARMEDKSKLGKPV 476

Query: 46   KI----GTPVKSTE 17
            KI     TP K ++
Sbjct: 477  KIKVRPETPPKGSD 490


>ref|XP_006358437.1| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1-like
            [Solanum tuberosum]
          Length = 488

 Score =  460 bits (1183), Expect = e-127
 Identities = 235/427 (55%), Positives = 281/427 (65%), Gaps = 5/427 (1%)
 Frame = -1

Query: 1306 DEK----YLQCCWPGGVKRPLKIXXXXXXXXXXXXXXXXXXXXGHHCTAFYLKIDQDYVY 1139
            DEK    YL C  P   K PLK+                    GH CTAFYLKI     +
Sbjct: 66   DEKINPTYLSCSMPH--KNPLKVAVLVSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDF 123

Query: 1138 ENYMPGCPWEDDLKYVRSVCEQAGVDLQRIDFTDRYWKSVVSYMISEYKRGFTPNPDILC 959
            ENY   CPWEDDLKY ++VC+Q  V L+ +  TD YW +VVSY+I EYK G TPNPD+LC
Sbjct: 124  ENYWSECPWEDDLKYAKAVCDQVDVPLEVVHLTDEYWNNVVSYIIEEYKCGRTPNPDVLC 183

Query: 958  NTRIKFGEFLDYIDTMNFDYIASGHYARVQHSGTTSKDTSAILCLSLDNVKDQTYFXXXX 779
            NTRIKFG F+D I  M FD++ASGHYA++ H+ T   D  +IL LS D VKDQTYF    
Sbjct: 184  NTRIKFGAFMDAISGMEFDFVASGHYAKIVHASTAQLDEPSILKLSKDMVKDQTYFLSHL 243

Query: 778  XXXXXXXLIFPLGCIPKNEVRELAADMGLPNKDRKDSQGICFLGKVKFSEFVARHXXXXX 599
                   LIFPLGCI K+EVR LA    LPN+DRKDSQGICFLGK+KFSEFVARH     
Sbjct: 244  SQAQLKRLIFPLGCIQKDEVRTLAKSFNLPNQDRKDSQGICFLGKIKFSEFVARHIGESE 303

Query: 598  XXXXEAETGAFLGNHRGFWFYTIGQRQGLGLTGGPWYVVAKDVENNVIFVSRNYYSLEMK 419
                EAE G +LGNHRGFWFYTIGQRQGL L GGPWYVV KD++NNV++VSRNY+S++ K
Sbjct: 304  GIILEAENGDYLGNHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVYVSRNYFSVDKK 363

Query: 418  RSFFHVSNIRWIYAT-PEDFENIKCKVRHGPGFYDCKIEHAKDIYWKEHNLPHPVEDSTQ 242
            R  F V +++W+    P+    ++CKVRHGP FYDC             +L   V+   Q
Sbjct: 364  RRLFRVGSLKWLSGLFPKQINELQCKVRHGPSFYDC-------------SLVMEVDQHGQ 410

Query: 241  EVYTVTLSENDQGLAAGQFAAFYKDNICLGSGIIVGSSKDAYFPVCSKALEIAKIKDKSV 62
            EV  V LS +DQGLAAGQFAAFY    C+GSGII+ S  D  +P+C KALEIA+++DKS 
Sbjct: 411  EVAVVRLSGDDQGLAAGQFAAFYNGRTCIGSGIILESWDDQGYPICEKALEIARMEDKSK 470

Query: 61   LGKPIKI 41
            LGKP+KI
Sbjct: 471  LGKPVKI 477


>ref|XP_002298404.2| hypothetical protein POPTR_0001s26620g [Populus trichocarpa]
            gi|550348245|gb|EEE83209.2| hypothetical protein
            POPTR_0001s26620g [Populus trichocarpa]
          Length = 512

 Score =  460 bits (1183), Expect = e-127
 Identities = 232/434 (53%), Positives = 286/434 (65%), Gaps = 1/434 (0%)
 Frame = -1

Query: 1306 DEKYLQCCWPGGVKRPLKIXXXXXXXXXXXXXXXXXXXXGHHCTAFYLKIDQDYVYENYM 1127
            D  YL CC     K PL+I                    GHHCTAFYLKI     + N+ 
Sbjct: 71   DPYYLSCCMADKDK-PLRIAVLLSGGVDSSVALRLLHAAGHHCTAFYLKIWFQEDFNNFW 129

Query: 1126 PGCPWEDDLKYVRSVCEQAGVDLQRIDFTDRYWKSVVSYMISEYKRGFTPNPDILCNTRI 947
              CPWE+DLK+ + VC+Q GV L+ +  TD YW +VVSY+I EY+ G TPNPD+LCNTRI
Sbjct: 130  SECPWEEDLKFAKGVCDQVGVPLEVVHLTDEYWSNVVSYIIEEYRCGRTPNPDVLCNTRI 189

Query: 946  KFGEFLDYIDTMNFDYIASGHYARVQHSGTTSKDTSAILCLSLDNVKDQTYFXXXXXXXX 767
            KFG F+D I+ M FDY+ASGHYA V H  +   +  ++L LS D +KDQTYF        
Sbjct: 190  KFGAFMDAINNMEFDYVASGHYAHVIHPVSDQINKPSVLELSKDKLKDQTYFLSHLTQAQ 249

Query: 766  XXXLIFPLGCIPKNEVRELAADMGLPNKDRKDSQGICFLGKVKFSEFVARHXXXXXXXXX 587
               LIFPLGCIPK EVR LA+   LPNKDRKDSQGICFLGK+KFSEFV RH         
Sbjct: 250  LKRLIFPLGCIPKEEVRNLASKFDLPNKDRKDSQGICFLGKIKFSEFVGRHIGESEGVIL 309

Query: 586  EAETGAFLGNHRGFWFYTIGQRQGLGLTGGPWYVVAKDVENNVIFVSRNYYSLEMKRSFF 407
            EAETG FLG HRGFWFYTIGQRQGL L GGPWYVV KDV+NNV+FVSRNY+SL+ +R  F
Sbjct: 310  EAETGDFLGIHRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSLDKRRRSF 369

Query: 406  HVSNIRWIYATPEDF-ENIKCKVRHGPGFYDCKIEHAKDIYWKEHNLPHPVEDSTQEVYT 230
             V +++W+  +P D    ++CKVRHGP FYDC ++                EDS +EV  
Sbjct: 370  RVGSLKWLSGSPTDMTTQLQCKVRHGPTFYDCSVKIELG------------EDSPEEVVA 417

Query: 229  VTLSENDQGLAAGQFAAFYKDNICLGSGIIVGSSKDAYFPVCSKALEIAKIKDKSVLGKP 50
            V L E+DQGLAAGQF AFY+   C+GSG+I+ S  D  FPVC KALE+A+++DKS LGKP
Sbjct: 418  VHLCEDDQGLAAGQFTAFYQGRTCIGSGVILESWDDKGFPVCQKALELARLEDKSKLGKP 477

Query: 49   IKIGTPVKSTETKN 8
            +KI    ++++ ++
Sbjct: 478  VKIKVKPETSDQES 491


>ref|XP_002513510.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase,
            putative [Ricinus communis] gi|223547418|gb|EEF48913.1|
            tRNA
            (5-methylaminomethyl-2-thiouridylate)-methyltransferase,
            putative [Ricinus communis]
          Length = 497

 Score =  459 bits (1181), Expect = e-126
 Identities = 232/423 (54%), Positives = 282/423 (66%), Gaps = 1/423 (0%)
 Frame = -1

Query: 1306 DEKYLQCCWPGGVKRPLKIXXXXXXXXXXXXXXXXXXXXGHHCTAFYLKIDQDYVYENYM 1127
            +  YL CC P   K  L+I                    GHHCTAFYLKI     +EN+ 
Sbjct: 57   EPNYLSCCLPD--KNNLRIALLLSGGVDSSVALRLLHAAGHHCTAFYLKIWFQEDFENFW 114

Query: 1126 PGCPWEDDLKYVRSVCEQAGVDLQRIDFTDRYWKSVVSYMISEYKRGFTPNPDILCNTRI 947
              CPWE+DLKY ++VC+Q  + L+ +  TD YWK+VVSY+I EY+ G TPNPD+LCNTRI
Sbjct: 115  SECPWEEDLKYAKAVCDQVDIPLEVVHLTDEYWKNVVSYIIEEYRCGRTPNPDVLCNTRI 174

Query: 946  KFGEFLDYIDTMNFDYIASGHYARVQHSGTTSKDTSAILCLSLDNVKDQTYFXXXXXXXX 767
            KFG F+D I+ M FDY+ASGHYA+V H      +  ++L LS D VKDQTYF        
Sbjct: 175  KFGAFMDAINNMEFDYVASGHYAKVIHPSADEINQPSVLELSKDMVKDQTYFLSHLSQAQ 234

Query: 766  XXXLIFPLGCIPKNEVRELAADMGLPNKDRKDSQGICFLGKVKFSEFVARHXXXXXXXXX 587
               LIFPLG I K+EVR+LA    LPNKDRKDSQGICFLGK+KFSEFVARH         
Sbjct: 235  LRRLIFPLGGISKDEVRKLATLFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEMEGIML 294

Query: 586  EAETGAFLGNHRGFWFYTIGQRQGLGLTGGPWYVVAKDVENNVIFVSRNYYSLEMKRSFF 407
            EAETG FLGNHRGFWFYTIGQRQGL L GGPWYVV KDV+NNV+FVSRNY+S + +R   
Sbjct: 295  EAETGDFLGNHRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSFDKRRRLL 354

Query: 406  HVSNIRWIYAT-PEDFENIKCKVRHGPGFYDCKIEHAKDIYWKEHNLPHPVEDSTQEVYT 230
             V ++RW   + P++   ++CKVRHGPGFY+C +            +  P E   Q+V  
Sbjct: 355  RVGSLRWFNGSPPKETSELQCKVRHGPGFYNCSV------------VIEPGEVDDQDVAI 402

Query: 229  VTLSENDQGLAAGQFAAFYKDNICLGSGIIVGSSKDAYFPVCSKALEIAKIKDKSVLGKP 50
            V L E+DQGLAAGQFAAFYK   C+GSG+I+ S  D  FPVC +ALE+A+I+DKS LGKP
Sbjct: 403  VQLCEDDQGLAAGQFAAFYKGRTCIGSGVILESWNDQGFPVCERALELARIEDKSELGKP 462

Query: 49   IKI 41
            +KI
Sbjct: 463  VKI 465


>gb|EXB93298.1| tRNA-specific 2-thiouridylase mnmA [Morus notabilis]
          Length = 580

 Score =  458 bits (1179), Expect = e-126
 Identities = 235/432 (54%), Positives = 282/432 (65%), Gaps = 1/432 (0%)
 Frame = -1

Query: 1333 HEQKQWRPADEKYLQCCWPGGVKRPLKIXXXXXXXXXXXXXXXXXXXXGHHCTAFYLKID 1154
            H  K +    E YL C  P    + L++                    GH CTAFYLKI 
Sbjct: 108  HHSKSFDGDIEPYLSCSMP---HKRLRVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIW 164

Query: 1153 QDYVYENYMPGCPWEDDLKYVRSVCEQAGVDLQRIDFTDRYWKSVVSYMISEYKRGFTPN 974
                +EN+   CPWE+DLKY ++VC Q  V L+ +  TD YWK+VVSY+I EY+ G TPN
Sbjct: 165  FQEDFENFWSECPWEEDLKYAKAVCSQIEVPLEVVHLTDEYWKNVVSYIIDEYRCGRTPN 224

Query: 973  PDILCNTRIKFGEFLDYIDTMNFDYIASGHYARVQHSGTTSKDTSAILCLSLDNVKDQTY 794
            PD+LCNTRIKFG F+D I +M FDY+ASGHYA V HS T  +  +++L LS D VKDQTY
Sbjct: 225  PDVLCNTRIKFGSFMDAISSMEFDYVASGHYANVVHSFTNERGDASVLELSQDMVKDQTY 284

Query: 793  FXXXXXXXXXXXLIFPLGCIPKNEVRELAADMGLPNKDRKDSQGICFLGKVKFSEFVARH 614
            F           L+FPLGCI K EVR+LA    LPNKDRKDSQGICFLGK+KFSEFVARH
Sbjct: 285  FLSHLSQAQLKRLVFPLGCISKEEVRKLAKKFDLPNKDRKDSQGICFLGKIKFSEFVARH 344

Query: 613  XXXXXXXXXEAETGAFLGNHRGFWFYTIGQRQGLGLTGGPWYVVAKDVENNVIFVSRNYY 434
                     EAETG FLG HRGFWFYTIGQRQGL L GGPWYVV KDV+NNV+F SRNY+
Sbjct: 345  IGEKEGIILEAETGDFLGMHRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFASRNYF 404

Query: 433  SLEMKRSFFHVSNIRWIYATPEDFE-NIKCKVRHGPGFYDCKIEHAKDIYWKEHNLPHPV 257
            S + +R  F V +++W+   P D    ++CKVRHGPGFY+C                   
Sbjct: 405  SFDKRRRIFRVGSLKWLNGLPPDQTCQLQCKVRHGPGFYNCSFTMESG------------ 452

Query: 256  EDSTQEVYTVTLSENDQGLAAGQFAAFYKDNICLGSGIIVGSSKDAYFPVCSKALEIAKI 77
            E+S ++V  V LSE+DQGLA GQFAAFY+   CLGSGII+ S  D  FPVC KALEIAK+
Sbjct: 453  ENSGEDVAVVQLSEDDQGLAVGQFAAFYQGKSCLGSGIILESWDDQGFPVCEKALEIAKM 512

Query: 76   KDKSVLGKPIKI 41
            +DKS+LGKP+KI
Sbjct: 513  EDKSLLGKPVKI 524


>ref|XP_006850208.1| hypothetical protein AMTR_s00022p00251260 [Amborella trichopoda]
            gi|548853806|gb|ERN11789.1| hypothetical protein
            AMTR_s00022p00251260 [Amborella trichopoda]
          Length = 565

 Score =  458 bits (1178), Expect = e-126
 Identities = 239/439 (54%), Positives = 292/439 (66%), Gaps = 3/439 (0%)
 Frame = -1

Query: 1348 CLFPQHEQKQWRPADEKYLQCCWPGGVKRPLKIXXXXXXXXXXXXXXXXXXXXGHHCTAF 1169
            C  P++        +++YL+C  P   ++  K+                    GH CTAF
Sbjct: 97   CFLPENHYSGSFVPEKRYLECSLP---EKRSKVAVLVSGGVDSSVALRLLCYAGHSCTAF 153

Query: 1168 YLKIDQDYVYENYMPGCPWEDDLKYVRSVCEQAGVDLQRIDFTDRYWKSVVSYMISEYKR 989
            YLKI     +EN+   CPW++DLKY ++VC++  V L+ +  TD YW +VVS++ISEY+ 
Sbjct: 154  YLKIWFQEDFENFWSACPWDEDLKYAKAVCDEVHVPLEVVHLTDEYWNNVVSHIISEYRS 213

Query: 988  GFTPNPDILCNTRIKFGEFLDYIDTMNFDYIASGHYARVQHSGTTSKDTSAILCLSLDNV 809
            G TPNPD+LCNTRIKFG FLD I  M+FDY+ASGHYARV H      D S+ L LS D V
Sbjct: 214  GRTPNPDVLCNTRIKFGAFLDAIGNMDFDYVASGHYARVVHPPPDQPDASSALELSADKV 273

Query: 808  KDQTYFXXXXXXXXXXXLIFPLGCIPKNEVRELAADMGLPNKDRKDSQGICFLGKVKFSE 629
            KDQTYF           L+FPLGC+ K+EVR LAA MGLPNKDRKDSQGICFLGK+KFS+
Sbjct: 274  KDQTYFLSHLSQTQLNRLLFPLGCLSKDEVRRLAAKMGLPNKDRKDSQGICFLGKLKFSD 333

Query: 628  FVARHXXXXXXXXXEAETGAFLGNHRGFWFYTIGQRQGLGLTGGPWYVVAKDVENNVIFV 449
            FV RH         EAETG FLG H+G+WFYTIGQRQGL L GGPWYVV KD+ENNVIFV
Sbjct: 334  FVTRHIGEMEGVLLEAETGDFLGKHQGYWFYTIGQRQGLRLPGGPWYVVNKDIENNVIFV 393

Query: 448  SRNYYSLEMKRSFFHVSNIRWIY-ATPEDFENIKCKVRHGPGFYDC--KIEHAKDIYWKE 278
            SRNY+S + KR  F V +++W+  A PE  + ++CKVRHGP FYDC  KIE   D    E
Sbjct: 394  SRNYFSFDKKRRSFRVGSLKWLSGAPPEHLDQLQCKVRHGPCFYDCTFKIESRCD----E 449

Query: 277  HNLPHPVEDSTQEVYTVTLSENDQGLAAGQFAAFYKDNICLGSGIIVGSSKDAYFPVCSK 98
            H      EDS      + +SE+DQGLAAGQFAAFY+ N+C+GSGII+ S  D  F VCSK
Sbjct: 450  H------EDSA----LIHISEDDQGLAAGQFAAFYRKNVCIGSGIILESWDDQNFSVCSK 499

Query: 97   ALEIAKIKDKSVLGKPIKI 41
            ALE AK++DKS LG PIKI
Sbjct: 500  ALERAKMEDKSKLGLPIKI 518


>ref|XP_004142116.1| PREDICTED: tRNA-specific 2-thiouridylase MnmA-like [Cucumis sativus]
            gi|449524848|ref|XP_004169433.1| PREDICTED: tRNA-specific
            2-thiouridylase MnmA-like [Cucumis sativus]
          Length = 538

 Score =  458 bits (1178), Expect = e-126
 Identities = 233/432 (53%), Positives = 285/432 (65%), Gaps = 1/432 (0%)
 Frame = -1

Query: 1297 YLQCCWPGGVKRPLKIXXXXXXXXXXXXXXXXXXXXGHHCTAFYLKIDQDYVYENYMPGC 1118
            +L C  P     PLK+                    GH CTAFYLKI     +EN+   C
Sbjct: 72   FLSCSMP---TYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSEC 128

Query: 1117 PWEDDLKYVRSVCEQAGVDLQRIDFTDRYWKSVVSYMISEYKRGFTPNPDILCNTRIKFG 938
            PW+DDLKY ++VC+Q  V L+ +  T+ YW++VVSY+I EY+ G TPNPD+LCNTRIKFG
Sbjct: 129  PWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFG 188

Query: 937  EFLDYIDTMNFDYIASGHYARVQHSGTTSKDTSAILCLSLDNVKDQTYFXXXXXXXXXXX 758
             F+D I  M FDY+ASGHYA V H      D  ++L LS D VKDQTYF           
Sbjct: 189  AFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLSQNQLKR 248

Query: 757  LIFPLGCIPKNEVRELAADMGLPNKDRKDSQGICFLGKVKFSEFVARHXXXXXXXXXEAE 578
            L+FPLGCIPK+EVR+LAA   LPNKDRKDSQGICFLGK+KFSEFVARH         EAE
Sbjct: 249  LLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAE 308

Query: 577  TGAFLGNHRGFWFYTIGQRQGLGLTGGPWYVVAKDVENNVIFVSRNYYSLEMKRSFFHVS 398
            +G FLG HRGFWFYTIGQRQGL L GGPWYVV KD++NNV+FVSRNY+S + +R  F V 
Sbjct: 309  SGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRVFRVG 368

Query: 397  NIRWIYATPED-FENIKCKVRHGPGFYDCKIEHAKDIYWKEHNLPHPVEDSTQEVYTVTL 221
            +++W+   P D F  ++CKVRHGP FY C +         EH+     ED  ++V  V L
Sbjct: 369  SLKWLSGFPPDQFTELQCKVRHGPSFYSCNLT-------MEHS-----EDGHEDVAVVHL 416

Query: 220  SENDQGLAAGQFAAFYKDNICLGSGIIVGSSKDAYFPVCSKALEIAKIKDKSVLGKPIKI 41
            SE+DQGLAAGQFAAFYK   C+GSG+I+ S  D  FPVC KA+E A+++DKS LGKPIKI
Sbjct: 417  SEDDQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKI 476

Query: 40   GTPVKSTETKNK 5
                +S   ++K
Sbjct: 477  KVKPESGMKESK 488


>ref|XP_006307321.1| hypothetical protein CARUB_v10008942mg [Capsella rubella]
            gi|482576032|gb|EOA40219.1| hypothetical protein
            CARUB_v10008942mg [Capsella rubella]
          Length = 496

 Score =  457 bits (1176), Expect = e-126
 Identities = 229/430 (53%), Positives = 284/430 (66%), Gaps = 2/430 (0%)
 Frame = -1

Query: 1303 EKYLQCCWPGGVKRPLKIXXXXXXXXXXXXXXXXXXXXGHHCTAFYLKIDQDYVYENYMP 1124
            ++YL C  P   ++PL++                    GH CTAFYLKI     +EN+  
Sbjct: 69   DQYLSCSMP---EKPLRVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEGFENFWD 125

Query: 1123 GCPWEDDLKYVRSVCEQAGVDLQRIDFTDRYWKSVVSYMISEYKRGFTPNPDILCNTRIK 944
             CPWEDDLKY + VCEQ GV L+ +  TD YW+ VVSY+I EY+ G TPNPD+LCNTRIK
Sbjct: 126  QCPWEDDLKYAKHVCEQVGVPLEVVHLTDEYWERVVSYLIEEYRCGRTPNPDVLCNTRIK 185

Query: 943  FGEFLDYIDTMNFDYIASGHYARVQHSGTTSKDTSAILCLSLDNVKDQTYFXXXXXXXXX 764
            FG F+D I  M +DY+ASGHYA+V H      D+S++L LS D VKDQTYF         
Sbjct: 186  FGAFMDAISDMEYDYVASGHYAKVVHPPADQNDSSSLLELSQDMVKDQTYFLSHLSQTQL 245

Query: 763  XXLIFPLGCIPKNEVRELAADMGLPNKDRKDSQGICFLGKVKFSEFVARHXXXXXXXXXE 584
              L+FPLGC+ K+EVR+LA    LPNKDRKDSQGICFLGK+KFS+FV RH         E
Sbjct: 246  KRLLFPLGCVKKDEVRKLATQFDLPNKDRKDSQGICFLGKIKFSDFVGRHIGEMEGVILE 305

Query: 583  AETGAFLGNHRGFWFYTIGQRQGLGLTGGPWYVVAKDVENNVIFVSRNYYSLEMKRSFFH 404
            AETG FLGNHRGFWFYTIGQRQGL L GGPWYVV KD +NNV+F SRNYYS++ +R  F 
Sbjct: 306  AETGDFLGNHRGFWFYTIGQRQGLRLPGGPWYVVEKDTKNNVVFASRNYYSIDKRRRVFR 365

Query: 403  VSNIRWIYATPE-DFENIKCKVRHGPGFYDCKIEHAKDIYWKEHNLPHPVEDSTQEVYTV 227
            V ++RW+   P  +   ++CKVRHGPGFY C  E                 ++  +V  V
Sbjct: 366  VGSLRWLSGKPSANVSELRCKVRHGPGFYSCSFE----------------METKGDVAVV 409

Query: 226  TLSENDQGLAAGQFAAFYKDNICLGSGIIVGSSKDAYFPVCSKALEIAKIKDKSVLGKPI 47
             L E+DQGLAAGQFAAFY+   C+GSG+I+ S  D  FPVC+KAL++A ++DK+ LGKP+
Sbjct: 410  HLDEDDQGLAAGQFAAFYEGTTCIGSGVILESWDDQCFPVCAKALQLAAMEDKTKLGKPV 469

Query: 46   KIGT-PVKST 20
            KI T PV S+
Sbjct: 470  KIFTIPVTSS 479


>ref|XP_004247484.1| PREDICTED: tRNA-specific 2-thiouridylase MnmA-like [Solanum
            lycopersicum]
          Length = 489

 Score =  457 bits (1176), Expect = e-126
 Identities = 230/423 (54%), Positives = 280/423 (66%), Gaps = 1/423 (0%)
 Frame = -1

Query: 1306 DEKYLQCCWPGGVKRPLKIXXXXXXXXXXXXXXXXXXXXGHHCTAFYLKIDQDYVYENYM 1127
            D  YL C  P   K PLK+                    GH CTAFYLKI     +ENY 
Sbjct: 66   DPTYLSCSMPH--KNPLKVAVLVSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENYW 123

Query: 1126 PGCPWEDDLKYVRSVCEQAGVDLQRIDFTDRYWKSVVSYMISEYKRGFTPNPDILCNTRI 947
              CPWEDDLKY + VC+Q  V L+ +  TD YW +VVSY+I EYK G TPNPD+LCNTRI
Sbjct: 124  SECPWEDDLKYAKGVCDQVDVPLEVVHLTDEYWNNVVSYIIEEYKCGRTPNPDVLCNTRI 183

Query: 946  KFGEFLDYIDTMNFDYIASGHYARVQHSGTTSKDTSAILCLSLDNVKDQTYFXXXXXXXX 767
            KFG F+D I  M FD++ASGHYA++ H+ T   D  ++L LS D VKDQTYF        
Sbjct: 184  KFGAFMDAISGMEFDFVASGHYAKIVHASTEQLDEPSVLELSKDMVKDQTYFLSHLSQAQ 243

Query: 766  XXXLIFPLGCIPKNEVRELAADMGLPNKDRKDSQGICFLGKVKFSEFVARHXXXXXXXXX 587
               LIFPLGCI K+EVR LA    LPN++RKDSQGICFLGK+KFSEFVARH         
Sbjct: 244  LKRLIFPLGCIQKDEVRMLAKSFNLPNQERKDSQGICFLGKIKFSEFVARHIGESEGIIL 303

Query: 586  EAETGAFLGNHRGFWFYTIGQRQGLGLTGGPWYVVAKDVENNVIFVSRNYYSLEMKRSFF 407
            EAE G +LGNHRGFWFYTIGQRQGL L GGPWYVV KD++NNV++VSRNY+S++ KR  F
Sbjct: 304  EAENGDYLGNHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVYVSRNYFSVDKKRRLF 363

Query: 406  HVSNIRWIYAT-PEDFENIKCKVRHGPGFYDCKIEHAKDIYWKEHNLPHPVEDSTQEVYT 230
             V +++W+    P+  + ++CKVRHGPGFY+C             +L   V+   QEV  
Sbjct: 364  RVGSLKWLSGLFPKQIDELQCKVRHGPGFYNC-------------SLVMEVDQHGQEVAV 410

Query: 229  VTLSENDQGLAAGQFAAFYKDNICLGSGIIVGSSKDAYFPVCSKALEIAKIKDKSVLGKP 50
            V LS +DQGLAAGQFAAFY    C+GSGII+ S  D  +P+C +ALEIA+++DKS LGKP
Sbjct: 411  VRLSGDDQGLAAGQFAAFYDGRTCIGSGIILESWDDQGYPICERALEIARMEDKSKLGKP 470

Query: 49   IKI 41
            +KI
Sbjct: 471  VKI 473


>ref|XP_006280360.1| hypothetical protein CARUB_v10026287mg [Capsella rubella]
            gi|482549064|gb|EOA13258.1| hypothetical protein
            CARUB_v10026287mg [Capsella rubella]
          Length = 494

 Score =  457 bits (1175), Expect = e-126
 Identities = 230/430 (53%), Positives = 283/430 (65%), Gaps = 2/430 (0%)
 Frame = -1

Query: 1303 EKYLQCCWPGGVKRPLKIXXXXXXXXXXXXXXXXXXXXGHHCTAFYLKIDQDYVYENYMP 1124
            ++YL C  P   ++PL++                    GH CTAFYLKI     +EN+  
Sbjct: 67   DQYLSCSMP---EKPLRVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEGFENFWN 123

Query: 1123 GCPWEDDLKYVRSVCEQAGVDLQRIDFTDRYWKSVVSYMISEYKRGFTPNPDILCNTRIK 944
             CPWEDDLKY + VCEQ  V L+ +  TD YW+ VVSY+I EY+ G TPNPD+LCNTRIK
Sbjct: 124  QCPWEDDLKYAKHVCEQVNVPLEVVHLTDEYWEKVVSYIIEEYRCGRTPNPDVLCNTRIK 183

Query: 943  FGEFLDYIDTMNFDYIASGHYARVQHSGTTSKDTSAILCLSLDNVKDQTYFXXXXXXXXX 764
            FG F+D I  M +DY+ASGHYA+V H      D+S++L LS D VKDQTYF         
Sbjct: 184  FGAFMDAISDMEYDYVASGHYAKVVHPPADQNDSSSLLELSQDMVKDQTYFLSHLSQTQL 243

Query: 763  XXLIFPLGCIPKNEVRELAADMGLPNKDRKDSQGICFLGKVKFSEFVARHXXXXXXXXXE 584
              L+FPLGC+ K+EVR+LA    LPNKDRKDSQGICFLGK+KFS+FV  H         E
Sbjct: 244  KRLLFPLGCVKKDEVRKLATQFDLPNKDRKDSQGICFLGKIKFSDFVGTHIGEMEGVILE 303

Query: 583  AETGAFLGNHRGFWFYTIGQRQGLGLTGGPWYVVAKDVENNVIFVSRNYYSLEMKRSFFH 404
            AETG FLGNHRGFWFYTIGQRQGL L GGPWYVV KD +NNV+FVSRNYYS++ KR  F 
Sbjct: 304  AETGDFLGNHRGFWFYTIGQRQGLRLPGGPWYVVEKDTKNNVVFVSRNYYSIDKKRRIFR 363

Query: 403  VSNIRWIYATPED-FENIKCKVRHGPGFYDCKIEHAKDIYWKEHNLPHPVEDSTQEVYTV 227
            V ++RW+   P D    ++CKVRHGPGFY C  E                 ++  +V  V
Sbjct: 364  VGSLRWLCGKPSDNVSKLRCKVRHGPGFYSCSFE----------------MEAKGDVAVV 407

Query: 226  TLSENDQGLAAGQFAAFYKDNICLGSGIIVGSSKDAYFPVCSKALEIAKIKDKSVLGKPI 47
             L E+DQGLAAGQFAAFY+   C+GSG+I+ S  D  FPVC+KAL++A ++DK+ LGKP+
Sbjct: 408  HLDEDDQGLAAGQFAAFYEGTTCIGSGVILESWDDQCFPVCAKALQLAAMEDKTKLGKPV 467

Query: 46   KIGT-PVKST 20
            KI T PV S+
Sbjct: 468  KILTMPVTSS 477


>ref|XP_004507093.1| PREDICTED: tRNA-specific 2-thiouridylase MnmA-like [Cicer arietinum]
          Length = 484

 Score =  453 bits (1165), Expect = e-124
 Identities = 229/422 (54%), Positives = 277/422 (65%), Gaps = 1/422 (0%)
 Frame = -1

Query: 1303 EKYLQCCWPGGVKRPLKIXXXXXXXXXXXXXXXXXXXXGHHCTAFYLKIDQDYVYENYMP 1124
            + YL+C  P     PL++                    GH CTAFYLKI     +EN+  
Sbjct: 57   DPYLRCSMP---HNPLRVAVLISGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWS 113

Query: 1123 GCPWEDDLKYVRSVCEQAGVDLQRIDFTDRYWKSVVSYMISEYKRGFTPNPDILCNTRIK 944
             CPWEDDLKY + VC Q  V L+ +  TD YWK+VVSY+I EY+ G TPNPD+LCNTRIK
Sbjct: 114  ECPWEDDLKYAKDVCNQVEVPLEVVHLTDEYWKNVVSYIIEEYQCGRTPNPDVLCNTRIK 173

Query: 943  FGEFLDYIDTMNFDYIASGHYARVQHSGTTSKDTSAILCLSLDNVKDQTYFXXXXXXXXX 764
            FG FLD I  M FDY+ASGHYA V H      D  + L LS D VKDQTYF         
Sbjct: 174  FGAFLDAISDMGFDYVASGHYANVIHPSADQMDGPSTLELSQDMVKDQTYFLSHLSQSQL 233

Query: 763  XXLIFPLGCIPKNEVRELAADMGLPNKDRKDSQGICFLGKVKFSEFVARHXXXXXXXXXE 584
              L+FPLGCIPK+EVR LA    LPNKDRKDSQGICFLGK+KFSEFVARH         E
Sbjct: 234  KQLLFPLGCIPKDEVRRLATKFDLPNKDRKDSQGICFLGKIKFSEFVARHIGEKEGIILE 293

Query: 583  AETGAFLGNHRGFWFYTIGQRQGLGLTGGPWYVVAKDVENNVIFVSRNYYSLEMKRSFFH 404
            AETG FLG HRGFWFYTIGQRQGL L GGPWYVV KDV+NNV+FVSRNY+S++ +R  F 
Sbjct: 294  AETGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSIDKRRRVFR 353

Query: 403  VSNIRWIYA-TPEDFENIKCKVRHGPGFYDCKIEHAKDIYWKEHNLPHPVEDSTQEVYTV 227
            V +++W+    P     ++CKVRHGPGFYDC ++        E    +P++ +      V
Sbjct: 354  VGSLKWLSGLPPSQTSQLRCKVRHGPGFYDCSLQ-------MELEGENPIDSA-----VV 401

Query: 226  TLSENDQGLAAGQFAAFYKDNICLGSGIIVGSSKDAYFPVCSKALEIAKIKDKSVLGKPI 47
             +SE+DQGLAAGQF AFY+   C+GSG+I+ S  D  FPVC+KALEIA+++DKS +G P+
Sbjct: 402  RISEDDQGLAAGQFTAFYEGRTCIGSGVILESWDDQGFPVCTKALEIARMEDKSKIGNPV 461

Query: 46   KI 41
            KI
Sbjct: 462  KI 463


>ref|XP_004301461.1| PREDICTED: tRNA-specific 2-thiouridylase MnmA-like [Fragaria vesca
            subsp. vesca]
          Length = 475

 Score =  453 bits (1165), Expect = e-124
 Identities = 227/422 (53%), Positives = 283/422 (67%), Gaps = 3/422 (0%)
 Frame = -1

Query: 1297 YLQCCWPGGVKRPLKIXXXXXXXXXXXXXXXXXXXXGHHCTAFYLKIDQDYVYENYMPGC 1118
            YL C  P   K+PLK+                    GH CTAFYLKI     +EN+   C
Sbjct: 63   YLSCSMPH--KKPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEGFENFWSEC 120

Query: 1117 PWEDDLKYVRSVCEQAGVDLQRIDFTDRYWKSVVSYMISEYKRGFTPNPDILCNTRIKFG 938
            PWE+DL Y ++VC Q  V L+ +  TD YWK+VVSY+I EY+ G TPNPD+LCNTRIKFG
Sbjct: 121  PWEEDLNYAKAVCNQVDVPLEVVHLTDEYWKNVVSYIIEEYRCGRTPNPDVLCNTRIKFG 180

Query: 937  EFLDYIDTMNFDYIASGHYARVQHSGTTSKDTSAILCLSLDNVKDQTYFXXXXXXXXXXX 758
             F+D ID+M FDY+ASGHYA+V H      D  ++L LS D VKDQTYF           
Sbjct: 181  VFMDAIDSMEFDYVASGHYAKVVHLSADQTDKDSVLELSQDMVKDQTYFLSHLSQDQLKK 240

Query: 757  LIFPLGCIPKNEVRELAADMGLPNKDRKDSQGICFLGKVKFSEFVARHXXXXXXXXXEAE 578
            LIFPLGC+ K++VR+LA    LPNK+RKDSQGICFLGKV+FSEF+ +H         EAE
Sbjct: 241  LIFPLGCLSKDKVRKLATKFDLPNKERKDSQGICFLGKVRFSEFIGKHIGEKEGVILEAE 300

Query: 577  TGAFLGNHRGFWFYTIGQRQGLGLTGGPWYVVAKDVENNVIFVSRNYYSLEMKRSFFHVS 398
            TG  LG HRGFWFYTIGQRQGL L GGPWYVV KD+++NV+FVSRNYYS++ +R  F V 
Sbjct: 301  TGDLLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKHNVVFVSRNYYSVDKRRRLFRVG 360

Query: 397  NIRWIYAT-PEDFENIKCKVRHGPGFYDC--KIEHAKDIYWKEHNLPHPVEDSTQEVYTV 227
            +++W++ + P     ++CKVRHGP F +C  KIEH               ED  ++V  V
Sbjct: 361  SLKWLHGSPPAQISQLQCKVRHGPRFCNCTVKIEHG--------------EDGNEDVAVV 406

Query: 226  TLSENDQGLAAGQFAAFYKDNICLGSGIIVGSSKDAYFPVCSKALEIAKIKDKSVLGKPI 47
            +LSE+DQGLAAGQFAAFY+   CLGSG+I+ S  D  FPVC+KALE A+++DKS+LGKP+
Sbjct: 407  SLSEDDQGLAAGQFAAFYQGRTCLGSGVILESWDDQGFPVCTKALENARMEDKSLLGKPV 466

Query: 46   KI 41
            KI
Sbjct: 467  KI 468


>ref|XP_006393065.1| hypothetical protein EUTSA_v10011432mg [Eutrema salsugineum]
            gi|557089643|gb|ESQ30351.1| hypothetical protein
            EUTSA_v10011432mg [Eutrema salsugineum]
          Length = 488

 Score =  451 bits (1161), Expect = e-124
 Identities = 233/435 (53%), Positives = 286/435 (65%), Gaps = 4/435 (0%)
 Frame = -1

Query: 1303 EKYLQCCWPGGVKRPLKIXXXXXXXXXXXXXXXXXXXXGHHCTAFYLKIDQDYVYENYMP 1124
            ++YL C  P   ++PL++                    GH CTAFYLKI     +EN+  
Sbjct: 60   DQYLSCSMP---EKPLRVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEGFENFWN 116

Query: 1123 GCPWEDDLKYVRSVCEQAGVDLQRIDFTDRYWKSVVSYMISEYKRGFTPNPDILCNTRIK 944
             CPWEDDLKY + VCEQ  V L+ +  TD YW+ VVSY+I EY+ G TPNPD+LCNTRIK
Sbjct: 117  QCPWEDDLKYAKHVCEQVDVPLEVVHLTDEYWERVVSYIIEEYRCGRTPNPDVLCNTRIK 176

Query: 943  FGEFLDYIDTMNFDYIASGHYARVQHSGTTSK-DTSAILCLSLDNVKDQTYFXXXXXXXX 767
            FG F+D I  M +DYIASGHYA+V H     + D+S++L LS D VKDQTYF        
Sbjct: 177  FGAFMDTISDMEYDYIASGHYAKVVHPPPADQNDSSSLLELSQDMVKDQTYFLSHLSQTQ 236

Query: 766  XXXLIFPLGCIPKNEVRELAADMGLPNKDRKDSQGICFLGKVKFSEFVARHXXXXXXXXX 587
               L+FPLGC+ K EVR+LA    LPNKDRKDSQGICFLGK+KFS+FV RH         
Sbjct: 237  LKRLLFPLGCVKKEEVRKLATKFDLPNKDRKDSQGICFLGKIKFSDFVGRHIGEMEGIIL 296

Query: 586  EAETGAFLGNHRGFWFYTIGQRQGLGLTGGPWYVVAKDVENNVIFVSRNYYSLEMKRSFF 407
            EAETG FLGNHRGFWFYTIGQRQGL L GGPWYVV KD +NNV+FVSRNYYS++ +R  F
Sbjct: 297  EAETGDFLGNHRGFWFYTIGQRQGLRLPGGPWYVVEKDTKNNVVFVSRNYYSIDKRRRIF 356

Query: 406  HVSNIRWIYATPE-DFENIKCKVRHGPGFYDCKIEHAKDIYWKEHNLPHPVEDSTQEVYT 230
             V ++RW+   P  +   ++CKVRHGPGFY C  E                 ++  +V  
Sbjct: 357  RVGSLRWLSGKPSGNVSQLRCKVRHGPGFYSCSFE----------------MEAQGDVAV 400

Query: 229  VTLSENDQGLAAGQFAAFYKDNICLGSGIIVGSSKDAYFPVCSKALEIAKIKDKSVLGKP 50
            V L E+DQGLAAGQFAAFY+   C+GSG+I+ S  D  FPVC+KALE+A ++DK+ LGKP
Sbjct: 401  VHLDEDDQGLAAGQFAAFYEGTTCIGSGVILESWDDQCFPVCAKALELAAMEDKTKLGKP 460

Query: 49   IKIGT-PV-KSTETK 11
            +KI T PV  S ET+
Sbjct: 461  VKIMTMPVTPSVETE 475


>dbj|BAD95081.1| hypothetical protein [Arabidopsis thaliana]
          Length = 497

 Score =  451 bits (1159), Expect = e-124
 Identities = 226/424 (53%), Positives = 275/424 (64%), Gaps = 1/424 (0%)
 Frame = -1

Query: 1303 EKYLQCCWPGGVKRPLKIXXXXXXXXXXXXXXXXXXXXGHHCTAFYLKIDQDYVYENYMP 1124
            ++YL C  P   ++PL++                    GH CTAFYLKI     +EN+  
Sbjct: 70   DQYLSCSMP---EKPLRVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEGFENFWN 126

Query: 1123 GCPWEDDLKYVRSVCEQAGVDLQRIDFTDRYWKSVVSYMISEYKRGFTPNPDILCNTRIK 944
             CPWEDDLKY + VCEQ  V L+ +  TD YW+ VVSY+I EY+ G TPNPD+LCNTRIK
Sbjct: 127  QCPWEDDLKYAKHVCEQVDVPLEVVHLTDEYWERVVSYIIEEYRCGRTPNPDVLCNTRIK 186

Query: 943  FGEFLDYIDTMNFDYIASGHYARVQHSGTTSKDTSAILCLSLDNVKDQTYFXXXXXXXXX 764
            FG F+D I  M +DY+ SGHYARV H      D S++L LS D VKDQTYF         
Sbjct: 187  FGAFMDAISDMEYDYVGSGHYARVVHPPADQNDASSVLELSQDMVKDQTYFLSHLSQTQL 246

Query: 763  XXLIFPLGCIPKNEVRELAADMGLPNKDRKDSQGICFLGKVKFSEFVARHXXXXXXXXXE 584
              L+FPLGC+ K+EVR+LA    LPNKDRKDSQGICFLGK+KFS+FV RH         E
Sbjct: 247  KRLLFPLGCVKKDEVRKLATQFDLPNKDRKDSQGICFLGKIKFSDFVCRHIGEMEGIILE 306

Query: 583  AETGAFLGNHRGFWFYTIGQRQGLGLTGGPWYVVAKDVENNVIFVSRNYYSLEMKRSFFH 404
            AE+G FLGNHRGFWFYTIGQRQGL L GGPWYVV KD +NNV+FVSRNYYS++ +R  F 
Sbjct: 307  AESGDFLGNHRGFWFYTIGQRQGLRLPGGPWYVVEKDTKNNVVFVSRNYYSIDKRRRIFR 366

Query: 403  VSNIRWIYATPE-DFENIKCKVRHGPGFYDCKIEHAKDIYWKEHNLPHPVEDSTQEVYTV 227
            V ++RW    P      ++CKVRHGPGFY C  E   D                 +V  V
Sbjct: 367  VGSLRWNSGKPSGKVRELRCKVRHGPGFYSCSFEMEGD----------------GDVAVV 410

Query: 226  TLSENDQGLAAGQFAAFYKDNICLGSGIIVGSSKDAYFPVCSKALEIAKIKDKSVLGKPI 47
             L E+DQGLAAGQFAAFY+   C+GSG+I+ S  D  FPVC+KAL++A ++DK+ LGKP+
Sbjct: 411  HLDEDDQGLAAGQFAAFYEGTTCIGSGVILESWDDQCFPVCAKALQLAALEDKTKLGKPV 470

Query: 46   KIGT 35
            KI T
Sbjct: 471  KIMT 474


>ref|XP_002894309.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase
            [Arabidopsis lyrata subsp. lyrata]
            gi|297340151|gb|EFH70568.1| tRNA
            (5-methylaminomethyl-2-thiouridylate)-methyltransferase
            [Arabidopsis lyrata subsp. lyrata]
          Length = 500

 Score =  450 bits (1158), Expect = e-124
 Identities = 225/424 (53%), Positives = 275/424 (64%), Gaps = 1/424 (0%)
 Frame = -1

Query: 1303 EKYLQCCWPGGVKRPLKIXXXXXXXXXXXXXXXXXXXXGHHCTAFYLKIDQDYVYENYMP 1124
            ++YL C  P   ++PL++                    GH CTAFYLKI     +EN+  
Sbjct: 73   DQYLSCSMP---EKPLRVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEGFENFWN 129

Query: 1123 GCPWEDDLKYVRSVCEQAGVDLQRIDFTDRYWKSVVSYMISEYKRGFTPNPDILCNTRIK 944
             CPWEDDLKY + VCEQ  V L+ +  TD YW+ VVSY+I EY+ G TPNPD+LCNTRIK
Sbjct: 130  QCPWEDDLKYAKHVCEQVDVPLEVVHLTDEYWERVVSYIIEEYRCGRTPNPDVLCNTRIK 189

Query: 943  FGEFLDYIDTMNFDYIASGHYARVQHSGTTSKDTSAILCLSLDNVKDQTYFXXXXXXXXX 764
            FG F+D I  M +DY+ SGHYA+V H      D S++L LS D VKDQTYF         
Sbjct: 190  FGAFMDAISDMEYDYVGSGHYAKVVHPPVDQNDASSVLELSQDMVKDQTYFLSHLSQTQL 249

Query: 763  XXLIFPLGCIPKNEVRELAADMGLPNKDRKDSQGICFLGKVKFSEFVARHXXXXXXXXXE 584
              LIFPLGC+ K+EVR+LA    LPNKDRKDSQGICFLGK+KFS+FV  H         E
Sbjct: 250  KRLIFPLGCVKKDEVRKLATQFDLPNKDRKDSQGICFLGKIKFSDFVCAHIGEMEGIILE 309

Query: 583  AETGAFLGNHRGFWFYTIGQRQGLGLTGGPWYVVAKDVENNVIFVSRNYYSLEMKRSFFH 404
            AETG FLGNHRGFWFYTIGQRQGL L GGPWYVV KD +NNV+FVSRNYYS++ +R  F 
Sbjct: 310  AETGDFLGNHRGFWFYTIGQRQGLRLPGGPWYVVEKDTKNNVVFVSRNYYSIDKRRRIFR 369

Query: 403  VSNIRWIYATPE-DFENIKCKVRHGPGFYDCKIEHAKDIYWKEHNLPHPVEDSTQEVYTV 227
            V ++RW+   P  +   ++CKVRHGP FY C  E   D                 +V  V
Sbjct: 370  VGSLRWLSGKPSGNVRELRCKVRHGPSFYSCSFEMEAD----------------GDVAVV 413

Query: 226  TLSENDQGLAAGQFAAFYKDNICLGSGIIVGSSKDAYFPVCSKALEIAKIKDKSVLGKPI 47
             L E+DQGLAAGQFAAFY+   C+GSG+I+ S  D  FPVC+KAL++A ++DK+ LGKP+
Sbjct: 414  HLDEDDQGLAAGQFAAFYEGTTCIGSGVILESWDDQCFPVCAKALQLAAMEDKTKLGKPV 473

Query: 46   KIGT 35
            KI T
Sbjct: 474  KIRT 477


>ref|NP_175542.2| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase
            [Arabidopsis thaliana] gi|332194528|gb|AEE32649.1| tRNA
            (5-methylaminomethyl-2-thiouridylate)-methyltransferase
            [Arabidopsis thaliana]
          Length = 497

 Score =  449 bits (1156), Expect = e-123
 Identities = 225/424 (53%), Positives = 275/424 (64%), Gaps = 1/424 (0%)
 Frame = -1

Query: 1303 EKYLQCCWPGGVKRPLKIXXXXXXXXXXXXXXXXXXXXGHHCTAFYLKIDQDYVYENYMP 1124
            ++YL C  P   ++PL++                    GH CTAFYLKI     +EN+  
Sbjct: 70   DQYLSCSMP---EKPLRVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEGFENFWN 126

Query: 1123 GCPWEDDLKYVRSVCEQAGVDLQRIDFTDRYWKSVVSYMISEYKRGFTPNPDILCNTRIK 944
             CPWEDDLKY + VCEQ  V L+ +  TD YW+ VVSY+I EY+ G TPNPD+LCNTRIK
Sbjct: 127  QCPWEDDLKYAKHVCEQVDVPLEVVHLTDEYWERVVSYIIEEYRCGRTPNPDVLCNTRIK 186

Query: 943  FGEFLDYIDTMNFDYIASGHYARVQHSGTTSKDTSAILCLSLDNVKDQTYFXXXXXXXXX 764
            FG F+D I  M +DY+ SGHYA+V H      D S++L LS D VKDQTYF         
Sbjct: 187  FGAFMDAISDMEYDYVGSGHYAKVVHPPADQNDASSVLELSQDMVKDQTYFLSHLSQTQL 246

Query: 763  XXLIFPLGCIPKNEVRELAADMGLPNKDRKDSQGICFLGKVKFSEFVARHXXXXXXXXXE 584
              L+FPLGC+ K+EVR+LA    LPNKDRKDSQGICFLGK+KFS+FV RH         E
Sbjct: 247  KRLLFPLGCVKKDEVRKLATQFDLPNKDRKDSQGICFLGKIKFSDFVCRHIGEMEGIILE 306

Query: 583  AETGAFLGNHRGFWFYTIGQRQGLGLTGGPWYVVAKDVENNVIFVSRNYYSLEMKRSFFH 404
            AE+G FLGNHRGFWFYTIGQRQGL L GGPWYVV KD +NNV+FVSRNYYS++ +R  F 
Sbjct: 307  AESGDFLGNHRGFWFYTIGQRQGLRLPGGPWYVVEKDTKNNVVFVSRNYYSIDKRRRIFR 366

Query: 403  VSNIRWIYATPE-DFENIKCKVRHGPGFYDCKIEHAKDIYWKEHNLPHPVEDSTQEVYTV 227
            V ++RW    P      ++CKVRHGPGFY C  E   D                 +V  V
Sbjct: 367  VGSLRWNSGKPSGKVRELRCKVRHGPGFYSCSFEMEGD----------------GDVAVV 410

Query: 226  TLSENDQGLAAGQFAAFYKDNICLGSGIIVGSSKDAYFPVCSKALEIAKIKDKSVLGKPI 47
             L E+DQGLAAGQFAAFY+   C+GSG+I+ S  D  FPVC+KAL++A ++DK+ LGKP+
Sbjct: 411  HLDEDDQGLAAGQFAAFYEGTTCIGSGVILESWDDQCFPVCAKALQLAALEDKTKLGKPV 470

Query: 46   KIGT 35
            KI T
Sbjct: 471  KIMT 474


>ref|XP_003539065.2| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1-like isoform
            X1 [Glycine max]
          Length = 502

 Score =  449 bits (1155), Expect = e-123
 Identities = 226/422 (53%), Positives = 276/422 (65%), Gaps = 1/422 (0%)
 Frame = -1

Query: 1303 EKYLQCCWPGGVKRPLKIXXXXXXXXXXXXXXXXXXXXGHHCTAFYLKIDQDYVYENYMP 1124
            ++YL+C  P     PL++                    GH CTAFYLKI     +EN+  
Sbjct: 63   DRYLRCSMPQN--SPLRVAVLVSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWS 120

Query: 1123 GCPWEDDLKYVRSVCEQAGVDLQRIDFTDRYWKSVVSYMISEYKRGFTPNPDILCNTRIK 944
             CPWEDDLKY + VC Q  V L+ +  TD YW +VVSY+I EY  G TPNPD+LCNTRIK
Sbjct: 121  ECPWEDDLKYAKDVCNQVDVPLEVVHLTDEYWNNVVSYLIEEYSSGRTPNPDVLCNTRIK 180

Query: 943  FGEFLDYIDTMNFDYIASGHYARVQHSGTTSKDTSAILCLSLDNVKDQTYFXXXXXXXXX 764
            FG FLD I  M FDY+ASGHYA V H  +  +D  ++L LS D VKDQTYF         
Sbjct: 181  FGAFLDAIGGMGFDYVASGHYANVIHPCSDRRDEPSVLELSQDMVKDQTYFLSHLSQSQL 240

Query: 763  XXLIFPLGCIPKNEVRELAADMGLPNKDRKDSQGICFLGKVKFSEFVARHXXXXXXXXXE 584
              L+FPLGCIPK+EVR+LA    LPNKDRKDSQGICFLGK++FSEFVARH         E
Sbjct: 241  KQLLFPLGCIPKDEVRKLATKFDLPNKDRKDSQGICFLGKIRFSEFVARHIGEREGVILE 300

Query: 583  AETGAFLGNHRGFWFYTIGQRQGLGLTGGPWYVVAKDVENNVIFVSRNYYSLEMKRSFFH 404
            AETG FLG HRGFWFYTIGQRQGL L GGPWYVV KDV+NNV+FVSRNY+S + +R  F 
Sbjct: 301  AETGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSFDKRRRVFR 360

Query: 403  VSNIRWIYATPE-DFENIKCKVRHGPGFYDCKIEHAKDIYWKEHNLPHPVEDSTQEVYTV 227
            V + +W+   P      ++CKVRHGPGFYDC ++   +   + H+              V
Sbjct: 361  VGSFKWLSGLPPGQTTQLQCKVRHGPGFYDCSLQMEVEADGQCHSA------------VV 408

Query: 226  TLSENDQGLAAGQFAAFYKDNICLGSGIIVGSSKDAYFPVCSKALEIAKIKDKSVLGKPI 47
             +SE+DQGLAAGQFAAFY+   C+GSG+I+    D  FPVC+KALEIA+++DKS +G P+
Sbjct: 409  RISEDDQGLAAGQFAAFYEGRTCIGSGVILEFWDDQSFPVCTKALEIARMEDKSKIGNPV 468

Query: 46   KI 41
            KI
Sbjct: 469  KI 470


>gb|ESW04011.1| hypothetical protein PHAVU_011G059600g [Phaseolus vulgaris]
          Length = 488

 Score =  447 bits (1151), Expect = e-123
 Identities = 226/421 (53%), Positives = 276/421 (65%), Gaps = 1/421 (0%)
 Frame = -1

Query: 1300 KYLQCCWPGGVKRPLKIXXXXXXXXXXXXXXXXXXXXGHHCTAFYLKIDQDYVYENYMPG 1121
            +YL+C  P     PL++                    GH CTAFYLKI     +EN+   
Sbjct: 62   RYLRCSLPQN--SPLRVAVLVSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSE 119

Query: 1120 CPWEDDLKYVRSVCEQAGVDLQRIDFTDRYWKSVVSYMISEYKRGFTPNPDILCNTRIKF 941
            CPWE+DLKY ++VCEQ  V L+ +  TD YW +VVSY+I EY  G TPNPD+LCNTRIKF
Sbjct: 120  CPWEEDLKYAKAVCEQVDVPLEVVHLTDEYWNNVVSYLIEEYSSGRTPNPDVLCNTRIKF 179

Query: 940  GEFLDYIDTMNFDYIASGHYARVQHSGTTSKDTSAILCLSLDNVKDQTYFXXXXXXXXXX 761
            G FLD I  M FDY+ASGHYA V H      +  ++L LS D VKDQTYF          
Sbjct: 180  GAFLDAIGGMGFDYVASGHYANVIHPCADGMNGPSVLELSQDMVKDQTYFLSHLSQSQLK 239

Query: 760  XLIFPLGCIPKNEVRELAADMGLPNKDRKDSQGICFLGKVKFSEFVARHXXXXXXXXXEA 581
             L+FPLGCIPK+EVR LA +  LPNKDRKDSQGICFLGK++FSEFVARH         EA
Sbjct: 240  QLLFPLGCIPKDEVRRLATEFDLPNKDRKDSQGICFLGKIRFSEFVARHIGEREGIILEA 299

Query: 580  ETGAFLGNHRGFWFYTIGQRQGLGLTGGPWYVVAKDVENNVIFVSRNYYSLEMKRSFFHV 401
            ETG FLG HRGFWFYTIGQRQGL L GGPWYVV KDV+NNV+FVSRNY+S + +R  F V
Sbjct: 300  ETGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSFDKRRRVFRV 359

Query: 400  SNIRWIYATPE-DFENIKCKVRHGPGFYDCKIEHAKDIYWKEHNLPHPVEDSTQEVYTVT 224
             + +W+   P      ++CKVRHGPGFYDC ++   +   + H+              V 
Sbjct: 360  GSFKWLSELPPGQTSQLQCKVRHGPGFYDCSLQMEVEGDGQGHSA------------VVR 407

Query: 223  LSENDQGLAAGQFAAFYKDNICLGSGIIVGSSKDAYFPVCSKALEIAKIKDKSVLGKPIK 44
            +SE+DQGLAAGQFAAFY+   CLGSG+I+    +  FPVC+KALEIA+++DKS+LG P+K
Sbjct: 408  ISEDDQGLAAGQFAAFYEGRTCLGSGVILEFWDEQSFPVCTKALEIARMEDKSILGNPVK 467

Query: 43   I 41
            I
Sbjct: 468  I 468