BLASTX nr result
ID: Ephedra27_contig00018058
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Ephedra27_contig00018058
(1045 letters)
Database: ./nr
37,332,560 sequences; 13,225,080,153 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_006386822.1| hypothetical protein POPTR_0002s22530g [Popu... 322 2e-85
ref|XP_002271798.1| PREDICTED: isoamylase 2, chloroplastic [Viti... 320 6e-85
ref|XP_002533079.1| isoamylase, putative [Ricinus communis] gi|2... 317 6e-84
gb|AAZ81836.1| isoamylase isoform 2 [Pisum sativum] 315 2e-83
ref|XP_006479763.1| PREDICTED: isoamylase 2, chloroplastic-like ... 314 3e-83
ref|XP_006444132.1| hypothetical protein CICLE_v10023436mg [Citr... 314 3e-83
ref|XP_006846640.1| hypothetical protein AMTR_s00156p00067780 [A... 307 4e-81
ref|XP_004493650.1| PREDICTED: isoamylase 2, chloroplastic-like ... 307 5e-81
dbj|BAD08581.1| 85kDa isoamylase [Hordeum vulgare subsp. vulgare... 307 5e-81
dbj|BAK02289.1| predicted protein [Hordeum vulgare subsp. vulgare] 307 5e-81
gb|EMJ02949.1| hypothetical protein PRUPE_ppa001199mg [Prunus pe... 306 9e-81
gb|EXB39337.1| Isoamylase 2 [Morus notabilis] 305 1e-80
ref|XP_004963786.1| PREDICTED: isoamylase 2, chloroplastic-like,... 304 3e-80
gb|EOX94834.1| Debranching enzyme 1 [Theobroma cacao] 304 4e-80
ref|XP_004164565.1| PREDICTED: LOW QUALITY PROTEIN: isoamylase 2... 304 4e-80
ref|XP_004136188.1| PREDICTED: isoamylase 2, chloroplastic-like ... 304 4e-80
dbj|BAF52942.1| isoamylase-type starch-debranching enzyme 2 [Pha... 304 4e-80
ref|XP_002441051.1| hypothetical protein SORBIDRAFT_09g019480 [S... 303 7e-80
gb|ACG48178.1| isoamylase-type starch debranching enzyme ISO2 [Z... 303 1e-79
emb|CAX51369.1| isoamylase [Hordeum vulgare subsp. vulgare] 300 8e-79
>ref|XP_006386822.1| hypothetical protein POPTR_0002s22530g [Populus trichocarpa]
gi|550345605|gb|ERP64619.1| hypothetical protein
POPTR_0002s22530g [Populus trichocarpa]
Length = 857
Score = 322 bits (825), Expect = 2e-85
Identities = 168/338 (49%), Positives = 215/338 (63%), Gaps = 4/338 (1%)
Frame = +1
Query: 1 LKGAHGEFLSRPPLLEAIAFDPFLSKAKIIADPCLPTVGACNDAAFPHWKKWAELNFKYK 180
L+ GE+LSRPP++EAIAFDP SK KIIAD P + AFPHWKKWAE+N K+
Sbjct: 521 LRSFGGEYLSRPPVVEAIAFDPLFSKTKIIADCWDPKELEAKETAFPHWKKWAEMNTKFC 580
Query: 181 DDIRRFVRGDRSQLSSFATRICGSGDEFTDGRGPSFSLNFITYSCGLPXXXXXXXXXXXX 360
DIR F+RG+ LS ATR+CGSGD F+ GRGP+FS NFIT + GLP
Sbjct: 581 YDIRNFLRGE-GLLSDLATRLCGSGDIFSSGRGPAFSFNFITRNFGLPLVDLVSFSGDEL 639
Query: 361 XXXXXWNCGEEGPTNRQSILETRLKQIRNYLFILFVSLGVPILNMGDECGHSKGGLVSLK 540
WNCGEEGPTN+ +ILE RLKQIRNYLF+LFVSLGVP+LNMGDECG S GG S
Sbjct: 640 ASELSWNCGEEGPTNKTTILERRLKQIRNYLFVLFVSLGVPVLNMGDECGQSSGGSTSYG 699
Query: 541 DRQPYIWDYLKSEFALQTTQFIGXXXXXXXXXXXXXXXXXXAKIENVNWHGELPGQPQWD 720
R+P+ W+ L + F +QTTQFI K EN++WHG P+W+
Sbjct: 700 SRKPFDWNALSTGFGIQTTQFISFLSSLRMRRSDLLQKRNFLKEENIDWHGSDQNPPRWE 759
Query: 721 NPDSLFLAVSLNSERNGAKPSSAA----GDIYIAFNSHPMPKFASLPELPLGISWCRLVD 888
+P FLA++L + G+ SS + GD++IAFN+ + +LPE+P G++W RLVD
Sbjct: 760 DPSCKFLAMTLKVDNPGSGLSSESSHVEGDMFIAFNAAGRSESVTLPEVPEGMAWHRLVD 819
Query: 889 TSLPFPETISIGRDTKESGVRFSTYEVQSHSCVLFEAH 1002
T+LPFP S + E +R YE++SHSC+L EA+
Sbjct: 820 TALPFPGFFS---NDSEPVIR-QPYEMKSHSCILLEAN 853
>ref|XP_002271798.1| PREDICTED: isoamylase 2, chloroplastic [Vitis vinifera]
Length = 882
Score = 320 bits (820), Expect = 6e-85
Identities = 168/341 (49%), Positives = 217/341 (63%), Gaps = 7/341 (2%)
Frame = +1
Query: 1 LKGAHGEFLSRPPLLEAIAFDPFLSKAKIIADPCLPTVGACNDAAFPHWKKWAELNFKYK 180
L+G HGE+LSRPPL+E IAFDP LSK KIIAD P + FPHWK+WAE+N ++
Sbjct: 542 LRGFHGEYLSRPPLVETIAFDPLLSKTKIIADCWDPRNMLPKEIRFPHWKRWAEVNTRFC 601
Query: 181 DDIRRFVRGDRSQLSSFATRICGSGDEFTDGRGPSFSLNFITYSCGLPXXXXXXXXXXXX 360
+D+R F+RG+ LS FATR+CGSGD F DGRGP+FS NF T + GLP
Sbjct: 602 NDVRNFLRGEG--LSDFATRLCGSGDIFMDGRGPAFSFNFTTKNFGLPLVDLVSFSSSEL 659
Query: 361 XXXXXWNCGEEGPTNRQSILETRLKQIRNYLFILFVSLGVPILNMGDECGHSKGGLVSLK 540
WNCG+EGPTN+ ++LE RLKQIRN+LFIL+VSLGVPILNMGDECG S GG +
Sbjct: 660 ASELSWNCGDEGPTNKTTVLERRLKQIRNFLFILYVSLGVPILNMGDECGQSSGGSPAYG 719
Query: 541 DRQPYIWDYLKSEFALQTTQFIGXXXXXXXXXXXXXXXXXXAKIENVNWHGELPGQPQWD 720
DR+P+ W+ +K+ F +QT QFI K E+++WHG P+WD
Sbjct: 720 DRKPFNWNSVKTGFGIQTIQFISFLSSLRSRRSDLLQRRSFLKEESIDWHGSDQSPPRWD 779
Query: 721 NPDSLFLAVSLNSER-NGAKP---SSAAGDIYIAFNSHPMPKFASLPELPLGISWCRLVD 888
+P S FLA++L +E G P SS GD++IAFN+ LP P G+ W RLVD
Sbjct: 780 DPSSKFLAMTLKAENMEGQLPSESSSIKGDLFIAFNTADRSVKVILPPPPTGMVWHRLVD 839
Query: 889 TSLPFPETISIGRDT---KESGVRFSTYEVQSHSCVLFEAH 1002
T+LPFP + + K+SG+ TY+++SHSC LFEA+
Sbjct: 840 TALPFPGFFTADGEAILKKKSGL--VTYKMESHSCALFEAN 878
>ref|XP_002533079.1| isoamylase, putative [Ricinus communis] gi|223527143|gb|EEF29318.1|
isoamylase, putative [Ricinus communis]
Length = 872
Score = 317 bits (811), Expect = 6e-84
Identities = 163/338 (48%), Positives = 209/338 (61%), Gaps = 5/338 (1%)
Frame = +1
Query: 1 LKGAHGEFLSRPPLLEAIAFDPFLSKAKIIADPCLPTVGACNDAAFPHWKKWAELNFKYK 180
L G HGE LSRPPL+EAIAFDP LSK KIIADP P + FPHWK+WAE+N K+
Sbjct: 531 LTGFHGEHLSRPPLVEAIAFDPILSKTKIIADPWHPEHRIPKETCFPHWKRWAEINPKFC 590
Query: 181 DDIRRFVRGDRSQLSSFATRICGSGDEFTDGRGPSFSLNFITYSCGLPXXXXXXXXXXXX 360
D+R F+RG+ S L ATR+CGSGD F++GRGP+FS N+I + GLP
Sbjct: 591 IDVRNFLRGE-SLLGDLATRLCGSGDIFSNGRGPAFSFNYIARNSGLPLVDLVSFSGGEL 649
Query: 361 XXXXXWNCGEEGPTNRQSILETRLKQIRNYLFILFVSLGVPILNMGDECGHSKGGLVSLK 540
WNCGEEGPTN+ ++LE RLKQIRNYLFIL+VSLGVP+LNMGDECG S G +S
Sbjct: 650 GSELSWNCGEEGPTNKTAVLERRLKQIRNYLFILYVSLGVPVLNMGDECGQSSRGSISYG 709
Query: 541 DRQPYIWDYLKSEFALQTTQFIGXXXXXXXXXXXXXXXXXXAKIENVNWHGELPGQPQWD 720
DR+P+ W+ L + F Q TQFI K EN++WHG P+W+
Sbjct: 710 DRKPFDWNALSTSFGNQMTQFISFLSSLRMRRSDLLQKRNFLKEENIDWHGNDQSPPRWE 769
Query: 721 NPDSLFLAVSLNSERN----GAKPSSAAGDIYIAFNSHPMPKFASLPELPLGISWCRLVD 888
+P FLA++L ++ ++PS+ GD+++AFN+ + LP +P G+ W RLVD
Sbjct: 770 DPTCKFLAMTLKIDKAESQLSSEPSNIKGDLFMAFNAAGHAESVILPPVPEGMIWRRLVD 829
Query: 889 TSLPFPETIS-IGRDTKESGVRFSTYEVQSHSCVLFEA 999
T+LPFP S G E Y++ SHSC LFEA
Sbjct: 830 TALPFPGFFSEDGEPVVEQIAGLIAYKMNSHSCTLFEA 867
>gb|AAZ81836.1| isoamylase isoform 2 [Pisum sativum]
Length = 857
Score = 315 bits (807), Expect = 2e-83
Identities = 162/339 (47%), Positives = 216/339 (63%), Gaps = 5/339 (1%)
Frame = +1
Query: 1 LKGAHGEFLSRPPLLEAIAFDPFLSKAKIIADPCLPTVGACNDAAFPHWKKWAELNFKYK 180
L+G HGE+LSRPPL+EAIAFDP L K KIIAD P + FPHW +WAE+N ++
Sbjct: 516 LRGYHGEYLSRPPLVEAIAFDPVLWKTKIIADCWDPNDMETKEIRFPHWMRWAEINTNFR 575
Query: 181 DDIRRFVRGDRSQLSSFATRICGSGDEFTDGRGPSFSLNFITYSCGLPXXXXXXXXXXXX 360
D+R F+RG+ S LS+ ATR+CGSGD ++DGRGP+FS N+I+ + GL
Sbjct: 576 SDVRNFLRGE-SLLSNLATRLCGSGDMYSDGRGPAFSFNYISGNFGLSLVDLVSFSNAGL 634
Query: 361 XXXXXWNCGEEGPTNRQSILETRLKQIRNYLFILFVSLGVPILNMGDECGHSKGGLVSLK 540
WNCGEEGPTN ++LE RLKQIRN+LFIL+VSLGVPILNMGDECGHS GG ++
Sbjct: 635 EAELSWNCGEEGPTNSTAVLERRLKQIRNFLFILYVSLGVPILNMGDECGHSSGGSLANG 694
Query: 541 DRQPYIWDYLKSEFALQTTQFIGXXXXXXXXXXXXXXXXXXAKIENVNWHGELPGQPQWD 720
+ +P+ W LK+ F QT QFI K EN+ W G P+W+
Sbjct: 695 NTKPFNWAALKTGFGKQTIQFISFLTSLRTRRSDLLQSRSFLKEENIEWRGIDEAPPKWE 754
Query: 721 NPDSLFLAVSLNSERNGAKPSSAA----GDIYIAFNSHPMPKFASLPELPLGISWCRLVD 888
+P FLA++L +ERN + SS + GD+++AFN+ P+ LP P G+SW R++D
Sbjct: 755 DPSCKFLAMTLKAERNEHQESSVSSDILGDLFVAFNADDCPETVVLPLPPEGMSWYRIID 814
Query: 889 TSLPFPE-TISIGRDTKESGVRFSTYEVQSHSCVLFEAH 1002
T+LPFP ++ G E STYE++S+SC+LFEA+
Sbjct: 815 TALPFPGFFLNNGDLVLEQMSGLSTYEMKSYSCILFEAN 853
>ref|XP_006479763.1| PREDICTED: isoamylase 2, chloroplastic-like isoform X1 [Citrus
sinensis] gi|568852193|ref|XP_006479764.1| PREDICTED:
isoamylase 2, chloroplastic-like isoform X2 [Citrus
sinensis] gi|568852195|ref|XP_006479765.1| PREDICTED:
isoamylase 2, chloroplastic-like isoform X3 [Citrus
sinensis]
Length = 889
Score = 314 bits (805), Expect = 3e-83
Identities = 164/343 (47%), Positives = 212/343 (61%), Gaps = 5/343 (1%)
Frame = +1
Query: 1 LKGAHGEFLSRPPLLEAIAFDPFLSKAKIIADPCLPTVGACNDAAFPHWKKWAELNFKYK 180
L+G HGE+LSRPPL+EAIAFDP LSKAK+IAD P A D FPHWK+WAELN +
Sbjct: 548 LRGFHGEYLSRPPLIEAIAFDPLLSKAKLIADYWDPHGVAPKDTRFPHWKRWAELNTNFC 607
Query: 181 DDIRRFVRGDRSQLSSFATRICGSGDEFTDGRGPSFSLNFITYSCGLPXXXXXXXXXXXX 360
+D+R F RG+ LS ATR+CGSGD F+DGRGP+FS N+I + GLP
Sbjct: 608 NDVRNFFRGE-GLLSDLATRLCGSGDIFSDGRGPAFSFNYIARNTGLPLVDLVSFSGGGL 666
Query: 361 XXXXXWNCGEEGPTNRQSILETRLKQIRNYLFILFVSLGVPILNMGDECGHSKGGLVSLK 540
WNCGEEGPT + ++LE RLKQIRN+LF+L+VSLGVPILNMGDECG S G S
Sbjct: 667 ASELSWNCGEEGPTTKTAVLERRLKQIRNFLFVLYVSLGVPILNMGDECGQSSWGSPSYA 726
Query: 541 DRQPYIWDYLKSEFALQTTQFIGXXXXXXXXXXXXXXXXXXAKIENVNWHGELPGQPQWD 720
DR+P+ W+ L + F +Q T+FI K EN++WHG P+W+
Sbjct: 727 DRKPFDWNALATGFGIQITEFISFLSSFRLKRSDLLEQRNFLKEENIDWHGSDHSPPRWE 786
Query: 721 NPDSLFLAVSLNSERNGAKPSSAA----GDIYIAFNSHPMPKFASLPELPLGISWCRLVD 888
+PD FLA+ L ++ ++ SS + GD+YIA N+ + LP P G++W LVD
Sbjct: 787 DPDCKFLAMRLKVDKAESQLSSESSQTKGDLYIACNAADHSESVVLPPPPEGMTWHHLVD 846
Query: 889 TSLPFPETISI-GRDTKESGVRFSTYEVQSHSCVLFEAHE*NE 1014
T+LPFP S G+ E TYE++ +SC LFEA N+
Sbjct: 847 TALPFPGFFSTEGKPVLEQMAGLYTYEMKPYSCTLFEASNGND 889
>ref|XP_006444132.1| hypothetical protein CICLE_v10023436mg [Citrus clementina]
gi|557546394|gb|ESR57372.1| hypothetical protein
CICLE_v10023436mg [Citrus clementina]
Length = 840
Score = 314 bits (805), Expect = 3e-83
Identities = 164/343 (47%), Positives = 212/343 (61%), Gaps = 5/343 (1%)
Frame = +1
Query: 1 LKGAHGEFLSRPPLLEAIAFDPFLSKAKIIADPCLPTVGACNDAAFPHWKKWAELNFKYK 180
L+G HGE+LSRPPL+EAIAFDP LSKAK+IAD P A D FPHWK+WAELN +
Sbjct: 499 LRGFHGEYLSRPPLIEAIAFDPLLSKAKLIADYWDPHGVAPKDTRFPHWKRWAELNTNFC 558
Query: 181 DDIRRFVRGDRSQLSSFATRICGSGDEFTDGRGPSFSLNFITYSCGLPXXXXXXXXXXXX 360
+D+R F RG+ LS ATR+CGSGD F+DGRGP+FS N+I + GLP
Sbjct: 559 NDVRNFFRGE-GLLSDLATRLCGSGDIFSDGRGPAFSFNYIARNTGLPLVDLVSFSGGGL 617
Query: 361 XXXXXWNCGEEGPTNRQSILETRLKQIRNYLFILFVSLGVPILNMGDECGHSKGGLVSLK 540
WNCGEEGPT + ++LE RLKQIRN+LF+L+VSLGVPILNMGDECG S G S
Sbjct: 618 ASELSWNCGEEGPTTKTAVLERRLKQIRNFLFVLYVSLGVPILNMGDECGQSSWGSPSYA 677
Query: 541 DRQPYIWDYLKSEFALQTTQFIGXXXXXXXXXXXXXXXXXXAKIENVNWHGELPGQPQWD 720
DR+P+ W+ L + F +Q T+FI K EN++WHG P+W+
Sbjct: 678 DRKPFDWNALATGFGIQITEFISFLSSFRLKRSDLLEQRNFLKEENIDWHGSDHSPPRWE 737
Query: 721 NPDSLFLAVSLNSERNGAKPSSAA----GDIYIAFNSHPMPKFASLPELPLGISWCRLVD 888
+PD FLA+ L ++ ++ SS + GD+YIA N+ + LP P G++W LVD
Sbjct: 738 DPDCKFLAMRLKVDKAESQLSSESSQTKGDLYIACNAADHSESVVLPPPPEGMTWHHLVD 797
Query: 889 TSLPFPETISI-GRDTKESGVRFSTYEVQSHSCVLFEAHE*NE 1014
T+LPFP S G+ E TYE++ +SC LFEA N+
Sbjct: 798 TALPFPGFFSTEGKPVLEQMAGLYTYEMKPYSCTLFEASNGND 840
>ref|XP_006846640.1| hypothetical protein AMTR_s00156p00067780 [Amborella trichopoda]
gi|548849492|gb|ERN08315.1| hypothetical protein
AMTR_s00156p00067780 [Amborella trichopoda]
Length = 926
Score = 307 bits (787), Expect = 4e-81
Identities = 155/335 (46%), Positives = 209/335 (62%), Gaps = 1/335 (0%)
Frame = +1
Query: 4 KGAHGEFLSRPPLLEAIAFDPFLSKAKIIADPCLPTVGACNDAAFPHWKKWAELNFKYKD 183
KG+ GE L+ PL+EAIAFDP LS AKIIAD P C + FPHWKKWAE+N ++
Sbjct: 591 KGSDGELLTLSPLIEAIAFDPILSHAKIIADCWSPLDMQCKEIHFPHWKKWAEMNARFCY 650
Query: 184 DIRRFVRGDRSQLSSFATRICGSGDEFTDGRGPSFSLNFITYSCGLPXXXXXXXXXXXXX 363
D+R F+RG+ LS+ ATR+CGSGD F+DGRGPSFS N+I + GLP
Sbjct: 651 DVRNFLRGE-GLLSNLATRLCGSGDIFSDGRGPSFSFNYIARNFGLPLVDLVSFSGSELS 709
Query: 364 XXXXWNCGEEGPTNRQSILETRLKQIRNYLFILFVSLGVPILNMGDECGHSKGGLVSLKD 543
WNCGEEGPT+ +LE+RLKQIRN+LFIL++SLGVP+LNMGDE G S GG +
Sbjct: 710 AELSWNCGEEGPTSTPVVLESRLKQIRNFLFILYISLGVPVLNMGDEYGQSTGGSTLYSN 769
Query: 544 RQPYIWDYLKSEFALQTTQFIGXXXXXXXXXXXXXXXXXXAKIENVNWHGELPGQPQWDN 723
R+ + W L+++F +QTTQ++ KIE+++WHGE QPQW+
Sbjct: 770 RKSFDWGSLRTDFGVQTTQYVTFLSSLRTKRSDLLQRKHFMKIEHLDWHGEDQSQPQWEA 829
Query: 724 PDSLFLAVSLNSERNGAKPSSAAGDIYIAFNSHPMPKFASLPELPLGISWCRLVDTSLPF 903
P S FLAV++N+ + + S GD+Y A N+H + A LP++ ++W LVDTSLP+
Sbjct: 830 PSSKFLAVTVNTGDDETETRSNGGDLYFAINAHGSSECAVLPQVSNNMAWFCLVDTSLPY 889
Query: 904 PETISI-GRDTKESGVRFSTYEVQSHSCVLFEAHE 1005
P S+ G + + Y ++ HSC LFEA +
Sbjct: 890 PGFFSMEGIPIDQPATSIAIYSMKPHSCTLFEARK 924
>ref|XP_004493650.1| PREDICTED: isoamylase 2, chloroplastic-like [Cicer arietinum]
Length = 858
Score = 307 bits (786), Expect = 5e-81
Identities = 160/339 (47%), Positives = 210/339 (61%), Gaps = 5/339 (1%)
Frame = +1
Query: 1 LKGAHGEFLSRPPLLEAIAFDPFLSKAKIIADPCLPTVGACNDAAFPHWKKWAELNFKYK 180
L+G HGE+LSRPPL+EAIAFDP L K KIIAD + FPHW +WAE+N +
Sbjct: 517 LRGFHGEYLSRPPLVEAIAFDPILWKTKIIADCWDLNAMEAKEIRFPHWMRWAEINTNFC 576
Query: 181 DDIRRFVRGDRSQLSSFATRICGSGDEFTDGRGPSFSLNFITYSCGLPXXXXXXXXXXXX 360
+D+R F+RG+ + LS+ ATR+CGSGD ++ GRGP+FS N+I + GL
Sbjct: 577 NDVRNFLRGE-NLLSNLATRLCGSGDMYSCGRGPAFSFNYIARNFGLSLVDLVSFSSADL 635
Query: 361 XXXXXWNCGEEGPTNRQSILETRLKQIRNYLFILFVSLGVPILNMGDECGHSKGGLVSLK 540
WNCGEEGPTN ++LE RLKQIRN+LFILFVSLGVP+LNMGDECG+S GG +
Sbjct: 636 DVELSWNCGEEGPTNNTAVLERRLKQIRNFLFILFVSLGVPVLNMGDECGYSSGGSPAYG 695
Query: 541 DRQPYIWDYLKSEFALQTTQFIGXXXXXXXXXXXXXXXXXXAKIENVNWHGELPGQPQWD 720
D +P+ W LK+ F QTTQFI K EN+ W G P+W+
Sbjct: 696 DTKPFNWAALKTGFGKQTTQFISFLTSLRKRRSDLLQSKSFLKEENIEWRGVDNAPPRWE 755
Query: 721 NPDSLFLAVSLNSERNGAKPSSAA----GDIYIAFNSHPMPKFASLPELPLGISWCRLVD 888
+P FLA++L +E+ + SS + GD++I FN+ P+ LP LP G+SW RLVD
Sbjct: 756 DPSCKFLAMNLKAEKGELQESSVSSDILGDLFIVFNADDHPETVVLPLLPEGVSWYRLVD 815
Query: 889 TSLPFPE-TISIGRDTKESGVRFSTYEVQSHSCVLFEAH 1002
T+LPFP ++ G E TYE++S+SC LFEA+
Sbjct: 816 TALPFPGFFLTNGDFVPEQISGLCTYEMKSYSCTLFEAN 854
>dbj|BAD08581.1| 85kDa isoamylase [Hordeum vulgare subsp. vulgare]
gi|326494128|dbj|BAJ85526.1| predicted protein [Hordeum
vulgare subsp. vulgare]
Length = 798
Score = 307 bits (786), Expect = 5e-81
Identities = 151/334 (45%), Positives = 210/334 (62%), Gaps = 1/334 (0%)
Frame = +1
Query: 1 LKGAHGEFLSRPPLLEAIAFDPFLSKAKIIADPCLPTVGACNDAAFPHWKKWAELNFKYK 180
++G+ GE+LSRPPLLE+IAFDP LSK KIIADP P + FPHWK+WAE+N ++
Sbjct: 458 VRGSRGEYLSRPPLLESIAFDPVLSKTKIIADPWSPLGISNVQFPFPHWKRWAEMNTRFS 517
Query: 181 DDIRRFVRGDRSQLSSFATRICGSGDEFTDGRGPSFSLNFITYSCGLPXXXXXXXXXXXX 360
D+R F++G+ + + ATR+CGSGD F++ RGP+FS N+++ + GL
Sbjct: 518 IDVRNFLKGE-ALIGDLATRLCGSGDLFSN-RGPAFSFNYVSRNSGLSLVDLVSFSNGDL 575
Query: 361 XXXXXWNCGEEGPTNRQSILETRLKQIRNYLFILFVSLGVPILNMGDECGHSKGGLVSLK 540
WNCGEEGP+ ++L+TRL+QIRN++FILFVSLG+P+LNMGDECGHS G S K
Sbjct: 576 ASESSWNCGEEGPSENSAVLQTRLRQIRNFIFILFVSLGIPVLNMGDECGHSSAGSTSYK 635
Query: 541 DRQPYIWDYLKSEFALQTTQFIGXXXXXXXXXXXXXXXXXXAKIENVNWHGELPGQPQWD 720
DR P W LK+ F + T FI K+EN++WHG +P+W+
Sbjct: 636 DRVPLKWKALKTSFVKEVTGFISFLAALRSRRGDVFQRREFLKLENISWHGNNLSEPRWE 695
Query: 721 NPDSLFLAVSLNSERNGAKPSSAAGDIYIAFNSHPMPKFASLPELPLGISWCRLVDTSLP 900
+P S FL + + +E +G P GD+YI FN+ P A+LP G W RLVDTSL
Sbjct: 696 DPTSKFLCMHIIAENDGNTPELTKGDLYICFNASEEPASATLPAPAEGSVWLRLVDTSLT 755
Query: 901 FPETISIGRDTK-ESGVRFSTYEVQSHSCVLFEA 999
FP + + + + + +S+YEV++HSCVLFE+
Sbjct: 756 FPSFFTTESNLEVQQVLGYSSYEVKAHSCVLFES 789
>dbj|BAK02289.1| predicted protein [Hordeum vulgare subsp. vulgare]
Length = 798
Score = 307 bits (786), Expect = 5e-81
Identities = 151/334 (45%), Positives = 210/334 (62%), Gaps = 1/334 (0%)
Frame = +1
Query: 1 LKGAHGEFLSRPPLLEAIAFDPFLSKAKIIADPCLPTVGACNDAAFPHWKKWAELNFKYK 180
++G+ GE+LSRPPLLE+IAFDP LSK KIIADP P + FPHWK+WAE+N ++
Sbjct: 458 VRGSRGEYLSRPPLLESIAFDPVLSKTKIIADPWSPLGISNVQFPFPHWKRWAEMNTRFS 517
Query: 181 DDIRRFVRGDRSQLSSFATRICGSGDEFTDGRGPSFSLNFITYSCGLPXXXXXXXXXXXX 360
D+R F++G+ + + ATR+CGSGD F++ RGP+FS N+++ + GL
Sbjct: 518 IDVRNFLKGE-ALIGDLATRLCGSGDLFSN-RGPAFSFNYVSRNSGLSLVDLVSFSNGDL 575
Query: 361 XXXXXWNCGEEGPTNRQSILETRLKQIRNYLFILFVSLGVPILNMGDECGHSKGGLVSLK 540
WNCGEEGP+ ++L+TRL+QIRN++FILFVSLG+P+LNMGDECGHS G S K
Sbjct: 576 ASESSWNCGEEGPSENSAVLQTRLRQIRNFIFILFVSLGIPVLNMGDECGHSSAGSTSYK 635
Query: 541 DRQPYIWDYLKSEFALQTTQFIGXXXXXXXXXXXXXXXXXXAKIENVNWHGELPGQPQWD 720
DR P W LK+ F + T FI K+EN++WHG +P+W+
Sbjct: 636 DRVPLKWKALKTSFVKEVTGFISFLAALRSRRGDVFQRREFLKLENISWHGNNLSEPRWE 695
Query: 721 NPDSLFLAVSLNSERNGAKPSSAAGDIYIAFNSHPMPKFASLPELPLGISWCRLVDTSLP 900
+P S FL + + +E +G P GD+YI FN+ P A+LP G W RLVDTSL
Sbjct: 696 DPTSKFLCMHIIAENDGNTPELTKGDLYICFNASEEPASATLPAPAEGSVWLRLVDTSLT 755
Query: 901 FPETISIGRDTK-ESGVRFSTYEVQSHSCVLFEA 999
FP + + + + + +S+YEV++HSCVLFE+
Sbjct: 756 FPSFFTTESNLEVQQVLGYSSYEVKAHSCVLFES 789
>gb|EMJ02949.1| hypothetical protein PRUPE_ppa001199mg [Prunus persica]
Length = 883
Score = 306 bits (784), Expect = 9e-81
Identities = 160/339 (47%), Positives = 212/339 (62%), Gaps = 6/339 (1%)
Frame = +1
Query: 1 LKGAHGEFLSRPPLLEAIAFDPFLSKAKIIADPCLPTVGACNDAAFPHWKKWAELNFKYK 180
L+G +GE+LSRPPL+EAIAFDP LSK KIIAD P A + FPHW++WAE+N K+
Sbjct: 543 LRGFNGEYLSRPPLVEAIAFDPLLSKTKIIADCWDPHGMAPKETHFPHWRRWAEVNTKFC 602
Query: 181 DDIRRFVRGDRSQLSSFATRICGSGDEFTDGRGPSFSLNFITYSCGLPXXXXXXXXXXXX 360
+D+R F+RG+ LS ATR+CG+GD F+DGRGP+F+ NFI+ + GLP
Sbjct: 603 NDVRNFLRGE-GLLSDLATRLCGNGDIFSDGRGPAFAFNFISRNSGLPLVDLVSFSGVEL 661
Query: 361 XXXXXWNCGEEGPTNRQSILETRLKQIRNYLFILFVSLGVPILNMGDECGHSKGGLVSLK 540
WNCGEEGPTN+ ++LE RLKQIRN+LFILFVSLGVP+LNMGDECG S GG +
Sbjct: 662 ASELSWNCGEEGPTNKTAVLERRLKQIRNFLFILFVSLGVPVLNMGDECGQSTGGSPAYS 721
Query: 541 DRQPYIWDYLKSEFALQTTQFIGXXXXXXXXXXXXXXXXXXAKIENVNWHGELPGQPQWD 720
DR+ + W+ L++ FA QTTQFI K EN+ W+ P+W+
Sbjct: 722 DRKAFDWNALETGFATQTTQFIAFLSSFRKRRSDLLQKRNFLKEENIGWYESDQTPPRWE 781
Query: 721 NPDSLFLAVSLNSERN-----GAKPSSAAGDIYIAFNSHPMPKFASLPELPLGISWCRLV 885
+P FLA+ L ++ + G + S + GD+++AF++ + LP G+ W RLV
Sbjct: 782 DPSRKFLAMRLKADEDEVNQPGDESSHSWGDLFVAFSAADHSETVVLPPPLEGMGWRRLV 841
Query: 886 DTSLPFPETISI-GRDTKESGVRFSTYEVQSHSCVLFEA 999
DT+LPFP S G E V YE++SHSC LFEA
Sbjct: 842 DTALPFPGFFSTDGEPVVEQIVGLFAYEMKSHSCALFEA 880
>gb|EXB39337.1| Isoamylase 2 [Morus notabilis]
Length = 825
Score = 305 bits (782), Expect = 1e-80
Identities = 158/338 (46%), Positives = 209/338 (61%), Gaps = 5/338 (1%)
Frame = +1
Query: 1 LKGAHGEFLSRPPLLEAIAFDPFLSKAKIIADPCLPTVGACNDAAFPHWKKWAELNFKYK 180
L+G+ GE+LS PPL+EAIAFDP LSK KIIAD P + FPHWK WAE+N K+
Sbjct: 486 LQGSRGEYLSHPPLVEAIAFDPLLSKTKIIADCWDPHNTVLEETRFPHWKTWAEMNMKFC 545
Query: 181 DDIRRFVRGDRSQLSSFATRICGSGDEFTDGRGPSFSLNFITYSCGLPXXXXXXXXXXXX 360
+D+R F+RG+ LSS ATR+CGSGD F+ GRGP+FS NFI+ + GLP
Sbjct: 546 NDVRNFLRGE-GLLSSLATRLCGSGDIFSKGRGPAFSFNFISRNSGLPLVDLVSFSSDKL 604
Query: 361 XXXXXWNCGEEGPTNRQSILETRLKQIRNYLFILFVSLGVPILNMGDECGHSKGGLVSLK 540
WNCGEEGPTN+ +LE RLKQ RN+LF+L+VSLGVP+LNMGDECG S GG +
Sbjct: 605 ASELSWNCGEEGPTNKTLVLERRLKQTRNFLFVLYVSLGVPVLNMGDECGQSTGGSPAYS 664
Query: 541 DRQPYIWDYLKSEFALQTTQFIGXXXXXXXXXXXXXXXXXXAKIENVNWHGELPGQPQWD 720
DR+ + W+ LKS F +QTT+FI K EN++WHG P+W+
Sbjct: 665 DRKSFDWNALKSSFGIQTTEFISFLSSLRRRRSDLLQNMNYLKEENIDWHGSDQSPPRWE 724
Query: 721 NPDSLFLAVSLNSERNGAK----PSSAAGDIYIAFNSHPMPKFASLPELPLGISWCRLVD 888
+P FLA+ L + + K +S GD++IAFN+ + + LP + G++W RLVD
Sbjct: 725 DPTCKFLAMRLRVDEDKDKAENQTTSGKGDLFIAFNAADLSESVILPPIAEGMAWHRLVD 784
Query: 889 TSLPFPE-TISIGRDTKESGVRFSTYEVQSHSCVLFEA 999
T+LPFP ++ G E+ YE++S S LFEA
Sbjct: 785 TALPFPGFFLTDGEPVPENVDGLLAYEMKSLSSTLFEA 822
>ref|XP_004963786.1| PREDICTED: isoamylase 2, chloroplastic-like, partial [Setaria
italica]
Length = 620
Score = 304 bits (779), Expect = 3e-80
Identities = 157/333 (47%), Positives = 207/333 (62%), Gaps = 1/333 (0%)
Frame = +1
Query: 1 LKGAHGEFLSRPPLLEAIAFDPFLSKAKIIADPCLPTVGACNDAAFPHWKKWAELNFKYK 180
++G GE LSRPPLLEAIAFDP LSK KIIADP P + FPHWK+WAE+N ++
Sbjct: 284 VRGPGGEGLSRPPLLEAIAFDPVLSKTKIIADPWSPLDISNVQFPFPHWKRWAEMNTRFS 343
Query: 181 DDIRRFVRGDRSQLSSFATRICGSGDEFTDGRGPSFSLNFITYSCGLPXXXXXXXXXXXX 360
D+R+F++G+ + +S ATR+CGSGD F+ RGP+FS N+++ + GL
Sbjct: 344 MDVRKFLKGE-ALISDLATRLCGSGDLFSS-RGPAFSFNYVSRNSGLTLVDLVSFSSDEL 401
Query: 361 XXXXXWNCGEEGPTNRQSILETRLKQIRNYLFILFVSLGVPILNMGDECGHSKGGLVSLK 540
WNCGEEGP+ ++L+TRL+QIRN+LFILFVSLG+P+LNMGDECGHS G S +
Sbjct: 402 GSEFSWNCGEEGPSESNAVLQTRLRQIRNFLFILFVSLGIPVLNMGDECGHSTAGSTSYE 461
Query: 541 DRQPYIWDYLKSEFALQTTQFIGXXXXXXXXXXXXXXXXXXAKIENVNWHGELPGQPQWD 720
DR P W LK+ F + T FI K+EN++W+G +PQW+
Sbjct: 462 DRGPLNWKALKTIFVKEVTGFISFLSALRSRRADIFQRREFLKLENIHWYGSNLSEPQWE 521
Query: 721 NPDSLFLAVSLNSERNGAKPSSAAGDIYIAFNSHPMPKFASLPELPLGISWCRLVDTSLP 900
+P S FL + +N E P S GD+YI FN++ A+LP L G W RLVDTSL
Sbjct: 522 DPTSNFLCMHINPELGENTPDSVRGDLYICFNANEESVSATLPALAEGSMWLRLVDTSLA 581
Query: 901 FPETISIGRDTKESGV-RFSTYEVQSHSCVLFE 996
FP S + K V FS+Y+V++HSCVLFE
Sbjct: 582 FPGFFSSESNPKVHQVPGFSSYQVKAHSCVLFE 614
>gb|EOX94834.1| Debranching enzyme 1 [Theobroma cacao]
Length = 867
Score = 304 bits (778), Expect = 4e-80
Identities = 161/338 (47%), Positives = 208/338 (61%), Gaps = 5/338 (1%)
Frame = +1
Query: 1 LKGAHGEFLSRPPLLEAIAFDPFLSKAKIIADPCLPTVGACNDAAFPHWKKWAELNFKYK 180
L+G +GE LSRPPL+EAIAFDP LSK KIIAD P + FPHWKKWAE+N K+
Sbjct: 526 LRGFYGEHLSRPPLIEAIAFDPLLSKTKIIADCWYPHDMMPKEIRFPHWKKWAEMNTKFC 585
Query: 181 DDIRRFVRGDRSQLSSFATRICGSGDEFTDGRGPSFSLNFITYSCGLPXXXXXXXXXXXX 360
DIR F+RG+ + LSS ATR+CGSGD F+DGRGP+FS N+I + GLP
Sbjct: 586 TDIRNFLRGEGA-LSSLATRLCGSGDIFSDGRGPAFSFNYIAKNFGLPLVDLVSFSKAEI 644
Query: 361 XXXXXWNCGEEGPTNRQSILETRLKQIRNYLFILFVSLGVPILNMGDECGHSKGGLVSLK 540
WNCG EGPTN+ ++LE RLKQIRN+LFILF+SLGVP+LNMGDECG S GG +S
Sbjct: 645 ASELSWNCGVEGPTNKTAVLERRLKQIRNFLFILFISLGVPVLNMGDECGQSSGGSLSYG 704
Query: 541 DRQPYIWDYLKSEFALQTTQFIGXXXXXXXXXXXXXXXXXXAKIENVNWHGELPGQPQWD 720
R+ W+ + + F +QTTQFI K EN+ W+G P W+
Sbjct: 705 SRKLLDWNAMTTGFGIQTTQFISFLSSLRMRRSDLLQKRSFLKEENIEWYGSDQSPPGWE 764
Query: 721 NPDSLFLAVSLNSERNGAKPSSAA----GDIYIAFNSHPMPKFASLPELPLGISWCRLVD 888
+P FLA++L +++ + SS A GD+ IA N+ + LP P G++W RLVD
Sbjct: 765 DPSCKFLAMTLKADKEENQLSSEASRLKGDLLIAINAADKAEIIILPPPPEGLAWRRLVD 824
Query: 889 TSLPFPETISI-GRDTKESGVRFSTYEVQSHSCVLFEA 999
T+LP+P S G+ E + YE++S SC LFEA
Sbjct: 825 TALPYPGFFSADGKAVLEQMMGLFAYEMKSLSCTLFEA 862
>ref|XP_004164565.1| PREDICTED: LOW QUALITY PROTEIN: isoamylase 2, chloroplastic-like
[Cucumis sativus]
Length = 885
Score = 304 bits (778), Expect = 4e-80
Identities = 159/343 (46%), Positives = 205/343 (59%), Gaps = 5/343 (1%)
Frame = +1
Query: 1 LKGAHGEFLSRPPLLEAIAFDPFLSKAKIIADPCLPTVGACNDAAFPHWKKWAELNFKYK 180
L+G HGE LSRPP +EAIAFDP LSK K++AD P + FPHWK+WAE+N K+
Sbjct: 546 LRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEVNSKFC 605
Query: 181 DDIRRFVRGDRSQLSSFATRICGSGDEFTDGRGPSFSLNFITYSCGLPXXXXXXXXXXXX 360
DIR F RG+ +SS ATR+CGSGD F+DGRGP+FS NFI + GLP
Sbjct: 606 SDIRDFFRGE-GLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFSNSNL 664
Query: 361 XXXXXWNCGEEGPTNRQSILETRLKQIRNYLFILFVSLGVPILNMGDECGHSKGGLVSLK 540
WNCGEEGPT+ +LE RLKQIRN++F+LFVSLGVP+LNMGDECG S GG V+
Sbjct: 665 ASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGSVAFN 724
Query: 541 DRQPYIWDYLKSEFALQTTQFIGXXXXXXXXXXXXXXXXXXAKIENVNWHGELPGQPQWD 720
D++ + WD LK++F QTTQFI K EN++W PQW+
Sbjct: 725 DKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSPPQWE 784
Query: 721 NPDSLFLAVSLNSERNG----AKPSSAAGDIYIAFNSHPMPKFASLPELPLGISWCRLVD 888
+ FLAV L +++ + +I++ FN+ + +LPE G SW R+VD
Sbjct: 785 DASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWFRVVD 844
Query: 889 TSLPFPETISI-GRDTKESGVRFSTYEVQSHSCVLFEAHE*NE 1014
T+LPFP S G +G TYE+Q+HSC LFEA N+
Sbjct: 845 TALPFPGFFSSDGELVPMTG--SVTYEIQAHSCALFEAKSAND 885
>ref|XP_004136188.1| PREDICTED: isoamylase 2, chloroplastic-like [Cucumis sativus]
Length = 885
Score = 304 bits (778), Expect = 4e-80
Identities = 159/343 (46%), Positives = 205/343 (59%), Gaps = 5/343 (1%)
Frame = +1
Query: 1 LKGAHGEFLSRPPLLEAIAFDPFLSKAKIIADPCLPTVGACNDAAFPHWKKWAELNFKYK 180
L+G HGE LSRPP +EAIAFDP LSK K++AD P + FPHWK+WAE+N K+
Sbjct: 546 LRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEVNSKFC 605
Query: 181 DDIRRFVRGDRSQLSSFATRICGSGDEFTDGRGPSFSLNFITYSCGLPXXXXXXXXXXXX 360
DIR F RG+ +SS ATR+CGSGD F+DGRGP+FS NFI + GLP
Sbjct: 606 SDIRDFFRGE-GLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFSNSNL 664
Query: 361 XXXXXWNCGEEGPTNRQSILETRLKQIRNYLFILFVSLGVPILNMGDECGHSKGGLVSLK 540
WNCGEEGPT+ +LE RLKQIRN++F+LFVSLGVP+LNMGDECG S GG V+
Sbjct: 665 ASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGSVAFN 724
Query: 541 DRQPYIWDYLKSEFALQTTQFIGXXXXXXXXXXXXXXXXXXAKIENVNWHGELPGQPQWD 720
D++ + WD LK++F QTTQFI K EN++W PQW+
Sbjct: 725 DKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSPPQWE 784
Query: 721 NPDSLFLAVSLNSERNG----AKPSSAAGDIYIAFNSHPMPKFASLPELPLGISWCRLVD 888
+ FLAV L +++ + +I++ FN+ + +LPE G SW R+VD
Sbjct: 785 DASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWFRVVD 844
Query: 889 TSLPFPETISI-GRDTKESGVRFSTYEVQSHSCVLFEAHE*NE 1014
T+LPFP S G +G TYE+Q+HSC LFEA N+
Sbjct: 845 TALPFPGFFSSDGELVPMTG--SVTYEIQAHSCALFEAKSAND 885
>dbj|BAF52942.1| isoamylase-type starch-debranching enzyme 2 [Phaseolus vulgaris]
gi|561035862|gb|ESW34392.1| hypothetical protein
PHAVU_001G148700g [Phaseolus vulgaris]
Length = 865
Score = 304 bits (778), Expect = 4e-80
Identities = 164/338 (48%), Positives = 202/338 (59%), Gaps = 5/338 (1%)
Frame = +1
Query: 1 LKGAHGEFLSRPPLLEAIAFDPFLSKAKIIADPCLPTVGACNDAAFPHWKKWAELNFKYK 180
L+G HGE+LSRPPL+EAIAFDP LSK KIIAD P + FPHW +WAE+N K+
Sbjct: 527 LRGFHGEYLSRPPLVEAIAFDPVLSKTKIIADCWDPHDTVAKEIHFPHWMRWAEMNAKFC 586
Query: 181 DDIRRFVRGDRSQLSSFATRICGSGDEFTDGRGPSFSLNFITYSCGLPXXXXXXXXXXXX 360
+D+R F RG+ + LS ATR+CGSGD F+ GRGP+FS N+I + G
Sbjct: 587 NDVRNFFRGE-NLLSDLATRLCGSGDMFSGGRGPAFSFNYIVRNFGFSLVDLVSFSSDDE 645
Query: 361 XXXXXWNCGEEGPTNRQSILETRLKQIRNYLFILFVSLGVPILNMGDECGHSKGGLVSLK 540
WNCGEEGPTN S+LE RLKQIRN+LFILFVSLGVP+LNMGDEC HS GG +
Sbjct: 646 LS---WNCGEEGPTNNTSVLERRLKQIRNFLFILFVSLGVPVLNMGDECAHSSGGFPAYD 702
Query: 541 DRQPYIWDYLKSEFALQTTQFIGXXXXXXXXXXXXXXXXXXAKIENVNWHGELPGQPQWD 720
D +P W L + F Q +QFI K EN+ W+G P+W+
Sbjct: 703 DIKPMTWSALTTGFGKQISQFIFFMSSLRRRRSDLLQRRSFLKEENIEWYGSDGAPPRWE 762
Query: 721 NPDSLFLAVSLNSE----RNGAKPSSAAGDIYIAFNSHPMPKFASLPELPLGISWCRLVD 888
+P FLA++L SE + S +GDI+IA N P+ LP P G+SW RLVD
Sbjct: 763 DPSCKFLAMTLKSEVAVLSESSVSSDISGDIFIALNVADEPESTVLPLPPEGMSWYRLVD 822
Query: 889 TSLPFPETI-SIGRDTKESGVRFSTYEVQSHSCVLFEA 999
TSLPFP S G E STY+++SHSC LFEA
Sbjct: 823 TSLPFPGFFSSSGEIVPEMKAGLSTYKMKSHSCALFEA 860
>ref|XP_002441051.1| hypothetical protein SORBIDRAFT_09g019480 [Sorghum bicolor]
gi|241946336|gb|EES19481.1| hypothetical protein
SORBIDRAFT_09g019480 [Sorghum bicolor]
Length = 800
Score = 303 bits (776), Expect = 7e-80
Identities = 154/334 (46%), Positives = 208/334 (62%), Gaps = 1/334 (0%)
Frame = +1
Query: 1 LKGAHGEFLSRPPLLEAIAFDPFLSKAKIIADPCLPTVGACNDAAFPHWKKWAELNFKYK 180
++G GE LSRPPLLEAIAFDP LSK KIIADP P + FPHWK+WAE+N ++
Sbjct: 464 VRGPGGEGLSRPPLLEAIAFDPVLSKTKIIADPWSPLDISNVQFPFPHWKRWAEMNTRFS 523
Query: 181 DDIRRFVRGDRSQLSSFATRICGSGDEFTDGRGPSFSLNFITYSCGLPXXXXXXXXXXXX 360
D+R+F++G+ + +S ATR+CGSGD F+ RGP+FS N+++ + GL
Sbjct: 524 MDVRKFLKGE-ALISDLATRLCGSGDLFSS-RGPAFSFNYVSRNSGLTLVDLVSFSSDEL 581
Query: 361 XXXXXWNCGEEGPTNRQSILETRLKQIRNYLFILFVSLGVPILNMGDECGHSKGGLVSLK 540
WNCGEEGP+ ++L+TRL+QIRN LFILF+SLG+P+LNMGDECGHS G S K
Sbjct: 582 GSEFSWNCGEEGPSENNAVLQTRLRQIRNLLFILFISLGIPVLNMGDECGHSAVGSTSYK 641
Query: 541 DRQPYIWDYLKSEFALQTTQFIGXXXXXXXXXXXXXXXXXXAKIENVNWHGELPGQPQWD 720
DR P W LK+ F + T FI K EN++W+G +P+W+
Sbjct: 642 DRGPLNWKALKTTFVKEVTGFISFLSALRSRRADIFQRREFLKFENIHWYGSDLSEPRWE 701
Query: 721 NPDSLFLAVSLNSERNGAKPSSAAGDIYIAFNSHPMPKFASLPELPLGISWCRLVDTSLP 900
+P S FL + +N+E + P S GD+YI FN++ A+LP + G W RLVDTSL
Sbjct: 702 DPTSNFLCMHINAELDEKLPDSTGGDLYICFNANEESASATLPAIAEGSMWLRLVDTSLA 761
Query: 901 FPETISIGRDTK-ESGVRFSTYEVQSHSCVLFEA 999
FP S G + + + FS+Y+V +HSCVLFE+
Sbjct: 762 FPGFFSRGSNPEIHKVLGFSSYQVNAHSCVLFES 795
>gb|ACG48178.1| isoamylase-type starch debranching enzyme ISO2 [Zea mays]
Length = 799
Score = 303 bits (775), Expect = 1e-79
Identities = 154/334 (46%), Positives = 209/334 (62%), Gaps = 1/334 (0%)
Frame = +1
Query: 1 LKGAHGEFLSRPPLLEAIAFDPFLSKAKIIADPCLPTVGACNDAAFPHWKKWAELNFKYK 180
++G GE LSRPPLLEAIAFDP LSK KIIADP P + FPHWK+WAE+N ++
Sbjct: 463 VRGPGGEGLSRPPLLEAIAFDPVLSKTKIIADPWSPLDISNVQFPFPHWKRWAEMNTRFS 522
Query: 181 DDIRRFVRGDRSQLSSFATRICGSGDEFTDGRGPSFSLNFITYSCGLPXXXXXXXXXXXX 360
D+R+F++G+ + +S ATR+CGSGD F+ RGP+FS N+++ + GL
Sbjct: 523 MDVRKFLKGE-ALISDLATRLCGSGDLFSS-RGPAFSFNYVSRNSGLTLVDLVSFSSDEL 580
Query: 361 XXXXXWNCGEEGPTNRQSILETRLKQIRNYLFILFVSLGVPILNMGDECGHSKGGLVSLK 540
WNCGEEGP+ ++L+TRL+QIRN+LFILF+SLG+P+LNMGDECG+S G S K
Sbjct: 581 ASEFSWNCGEEGPSENNAVLQTRLRQIRNFLFILFISLGIPVLNMGDECGNSAAGSTSYK 640
Query: 541 DRQPYIWDYLKSEFALQTTQFIGXXXXXXXXXXXXXXXXXXAKIENVNWHGELPGQPQWD 720
DR P W LK+ F + T FI K+EN++W+G +P W+
Sbjct: 641 DRGPLNWKALKTAFVKEVTGFISFLSALRSRRADIFQRCEFLKLENIHWYGSDLSEPCWE 700
Query: 721 NPDSLFLAVSLNSERNGAKPSSAAGDIYIAFNSHPMPKFASLPELPLGISWCRLVDTSLP 900
+P S FL + +N+E + P S GD+YI FN++ A+LP + G W RLVDTSL
Sbjct: 701 DPTSNFLCLHINAELDEKLPDSTGGDLYICFNANEESASATLPAIAEGSMWLRLVDTSLA 760
Query: 901 FPETISIGRDTKESGV-RFSTYEVQSHSCVLFEA 999
FP S G + V FS+Y+V++HSCVLFE+
Sbjct: 761 FPGFFSRGSSHETHQVLGFSSYQVKAHSCVLFES 794
>emb|CAX51369.1| isoamylase [Hordeum vulgare subsp. vulgare]
Length = 798
Score = 300 bits (767), Expect = 8e-79
Identities = 148/334 (44%), Positives = 207/334 (61%), Gaps = 1/334 (0%)
Frame = +1
Query: 1 LKGAHGEFLSRPPLLEAIAFDPFLSKAKIIADPCLPTVGACNDAAFPHWKKWAELNFKYK 180
++G+ GE+LSRPPLLE+IAFDP LSK ADP P + FPHWK+WAE+N ++
Sbjct: 458 VRGSRGEYLSRPPLLESIAFDPVLSKTGTRADPWSPLGISNVQFPFPHWKRWAEMNTRFS 517
Query: 181 DDIRRFVRGDRSQLSSFATRICGSGDEFTDGRGPSFSLNFITYSCGLPXXXXXXXXXXXX 360
D+R F++G+ + + ATR+CGSGD F++ RGP+FS N+++ + GL
Sbjct: 518 IDVRNFLKGE-ALIGDLATRLCGSGDLFSN-RGPAFSFNYVSRNSGLSLVDLVSFSNGDL 575
Query: 361 XXXXXWNCGEEGPTNRQSILETRLKQIRNYLFILFVSLGVPILNMGDECGHSKGGLVSLK 540
WNCGEEGP+ ++L+TRL+QIRN++FILFVSLG+P+LNMGDECGHS G S K
Sbjct: 576 ASESSWNCGEEGPSENSAVLQTRLRQIRNFIFILFVSLGIPVLNMGDECGHSSAGSTSYK 635
Query: 541 DRQPYIWDYLKSEFALQTTQFIGXXXXXXXXXXXXXXXXXXAKIENVNWHGELPGQPQWD 720
DR P W LK+ F + T FI K+EN++WHG +P+W+
Sbjct: 636 DRVPLKWKALKTSFVKEVTGFISFLAALRSRRGDVFQRREFLKLENISWHGNNLSEPRWE 695
Query: 721 NPDSLFLAVSLNSERNGAKPSSAAGDIYIAFNSHPMPKFASLPELPLGISWCRLVDTSLP 900
+P S FL + + +E +G P GD+YI FN+ P A+LP G W RLVDTSL
Sbjct: 696 DPTSKFLCMHIIAENDGNTPELTKGDLYICFNASEEPASATLPAPAEGSVWLRLVDTSLT 755
Query: 901 FPETISIGRDTK-ESGVRFSTYEVQSHSCVLFEA 999
FP + + + + + +S+YEV++HSCVLFE+
Sbjct: 756 FPSFFTTESNLEVQQVLGYSSYEVKAHSCVLFES 789