BLASTX nr result
ID: Ephedra27_contig00015351
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Ephedra27_contig00015351
(378 letters)
Database: ./nr
37,332,560 sequences; 13,225,080,153 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|ABK24009.1| unknown [Picea sitchensis] gi|224285222|gb|ACN403... 182 3e-44
gb|ABK21318.1| unknown [Picea sitchensis] 182 5e-44
ref|XP_004134048.1| PREDICTED: DAG protein, chloroplastic-like [... 168 6e-40
gb|AFW75308.1| hypothetical protein ZEAMMB73_861231 [Zea mays] 168 6e-40
ref|XP_002285392.1| PREDICTED: DAG protein, chloroplastic [Vitis... 168 8e-40
ref|NP_001056622.1| Os06g0116600 [Oryza sativa Japonica Group] g... 167 1e-39
dbj|BAC22214.1| putative plastid protein [Oryza sativa Japonica ... 167 1e-39
ref|XP_006656573.1| PREDICTED: DAG protein, chloroplastic-like, ... 165 5e-39
ref|XP_002312591.2| plastid developmental protein DAG [Populus t... 165 7e-39
ref|XP_002334594.1| predicted protein [Populus trichocarpa] 165 7e-39
gb|EMJ24941.1| hypothetical protein PRUPE_ppa010500mg [Prunus pe... 164 9e-39
ref|XP_002532881.1| DAG protein, chloroplast precursor, putative... 164 9e-39
ref|NP_001150208.1| DAG protein [Zea mays] gi|195637572|gb|ACG38... 164 9e-39
ref|XP_004965835.1| PREDICTED: uncharacterized protein At3g15000... 164 1e-38
ref|NP_001236832.1| uncharacterized protein LOC100306054 [Glycin... 163 1e-38
ref|XP_006345901.1| PREDICTED: DAG protein, chloroplastic-like [... 164 1e-38
ref|XP_004964371.1| PREDICTED: uncharacterized protein At3g15000... 164 1e-38
dbj|BAJ87670.1| predicted protein [Hordeum vulgare subsp. vulgare] 164 1e-38
ref|XP_002436376.1| hypothetical protein SORBIDRAFT_10g001390 [S... 164 1e-38
gb|EPS66780.1| hypothetical protein M569_07993 [Genlisea aurea] 163 2e-38
>gb|ABK24009.1| unknown [Picea sitchensis] gi|224285222|gb|ACN40337.1| unknown
[Picea sitchensis]
Length = 244
Score = 182 bits (463), Expect = 3e-44
Identities = 92/124 (74%), Positives = 102/124 (82%)
Frame = +1
Query: 1 TKEEMIDHYVKTLARVLGSEEAAKKAIYNVSCERYYGFGCEIDENTSNKLVGLPGVQFVL 180
TK+EMID Y++TLA+VLGSEEAAKK+IYNVSCERY+GFGC+IDE TSNKLVGLPGV FVL
Sbjct: 122 TKQEMIDCYIQTLAKVLGSEEAAKKSIYNVSCERYFGFGCQIDEETSNKLVGLPGVVFVL 181
Query: 181 PDSYVDPEYKDYGGELLVDGKIVPRTPERQRRVTPAPQRINSDXXXXXXXXXXXXXXEST 360
PDSYVD EYKDYGGELLVDGKIV R+PERQRRVTPAPQR N+D E+
Sbjct: 182 PDSYVDAEYKDYGGELLVDGKIVERSPERQRRVTPAPQR-NNDRPRYNDRTRYARRRENQ 240
Query: 361 VQGR 372
QGR
Sbjct: 241 NQGR 244
>gb|ABK21318.1| unknown [Picea sitchensis]
Length = 265
Score = 182 bits (461), Expect = 5e-44
Identities = 87/101 (86%), Positives = 95/101 (94%)
Frame = +1
Query: 1 TKEEMIDHYVKTLARVLGSEEAAKKAIYNVSCERYYGFGCEIDENTSNKLVGLPGVQFVL 180
TK+EMID Y++TLA+VLGSEEAAKK+IYNVSCERY+GFGC+IDE TSNKLVGLPGV FVL
Sbjct: 143 TKQEMIDCYIQTLAKVLGSEEAAKKSIYNVSCERYFGFGCQIDEETSNKLVGLPGVVFVL 202
Query: 181 PDSYVDPEYKDYGGELLVDGKIVPRTPERQRRVTPAPQRIN 303
PDSYVD EYKDYGGELLVDGKIV R+PERQRRVTPAPQR N
Sbjct: 203 PDSYVDAEYKDYGGELLVDGKIVERSPERQRRVTPAPQRNN 243
>ref|XP_004134048.1| PREDICTED: DAG protein, chloroplastic-like [Cucumis sativus]
gi|449517983|ref|XP_004166023.1| PREDICTED: DAG protein,
chloroplastic-like [Cucumis sativus]
Length = 243
Score = 168 bits (426), Expect = 6e-40
Identities = 78/101 (77%), Positives = 90/101 (89%)
Frame = +1
Query: 1 TKEEMIDHYVKTLARVLGSEEAAKKAIYNVSCERYYGFGCEIDENTSNKLVGLPGVQFVL 180
TK++MID Y++TLA+++GSEE AKK IYNVSCERY+GFGCE+DE TSNKL GLPGV FVL
Sbjct: 124 TKQQMIDCYIQTLAKIVGSEEEAKKRIYNVSCERYFGFGCELDEETSNKLEGLPGVLFVL 183
Query: 181 PDSYVDPEYKDYGGELLVDGKIVPRTPERQRRVTPAPQRIN 303
PDSYVDPEYKDYG ELLV+G+IV R+PERQRRV P PQR N
Sbjct: 184 PDSYVDPEYKDYGAELLVNGEIVQRSPERQRRVQPQPQRAN 224
>gb|AFW75308.1| hypothetical protein ZEAMMB73_861231 [Zea mays]
Length = 217
Score = 168 bits (426), Expect = 6e-40
Identities = 80/99 (80%), Positives = 88/99 (88%)
Frame = +1
Query: 1 TKEEMIDHYVKTLARVLGSEEAAKKAIYNVSCERYYGFGCEIDENTSNKLVGLPGVQFVL 180
TK++MID Y++TLA+VLGSEE AKK IYNVSCERY+GFGCEIDE TSNKL GLPGV FVL
Sbjct: 98 TKQQMIDCYIQTLAKVLGSEEEAKKKIYNVSCERYFGFGCEIDEETSNKLEGLPGVLFVL 157
Query: 181 PDSYVDPEYKDYGGELLVDGKIVPRTPERQRRVTPAPQR 297
PDSYVDPEYKDYG ELLV+G+IV R PERQRRV P PQR
Sbjct: 158 PDSYVDPEYKDYGAELLVNGEIVQRPPERQRRVEPVPQR 196
>ref|XP_002285392.1| PREDICTED: DAG protein, chloroplastic [Vitis vinifera]
gi|147779193|emb|CAN67991.1| hypothetical protein
VITISV_023920 [Vitis vinifera]
Length = 233
Score = 168 bits (425), Expect = 8e-40
Identities = 79/99 (79%), Positives = 89/99 (89%)
Frame = +1
Query: 1 TKEEMIDHYVKTLARVLGSEEAAKKAIYNVSCERYYGFGCEIDENTSNKLVGLPGVQFVL 180
TK++MID Y++TLA+V+GSEE AKK IYNVSCERY+GFGCEIDE TSNKL GLPGV FVL
Sbjct: 114 TKQQMIDCYIQTLAKVVGSEEEAKKKIYNVSCERYFGFGCEIDEETSNKLEGLPGVLFVL 173
Query: 181 PDSYVDPEYKDYGGELLVDGKIVPRTPERQRRVTPAPQR 297
PDSYVDPEYKDYG EL V+G+IV R+PERQRRV PAPQR
Sbjct: 174 PDSYVDPEYKDYGAELFVNGEIVQRSPERQRRVEPAPQR 212
>ref|NP_001056622.1| Os06g0116600 [Oryza sativa Japonica Group]
gi|55296199|dbj|BAD67917.1| putative DAL1 protein [Oryza
sativa Japonica Group] gi|113594662|dbj|BAF18536.1|
Os06g0116600 [Oryza sativa Japonica Group]
gi|218197457|gb|EEC79884.1| hypothetical protein
OsI_21391 [Oryza sativa Indica Group]
gi|222634859|gb|EEE64991.1| hypothetical protein
OsJ_19911 [Oryza sativa Japonica Group]
Length = 165
Score = 167 bits (424), Expect = 1e-39
Identities = 79/99 (79%), Positives = 88/99 (88%)
Frame = +1
Query: 1 TKEEMIDHYVKTLARVLGSEEAAKKAIYNVSCERYYGFGCEIDENTSNKLVGLPGVQFVL 180
TK++MID Y++TLA+VLGSEE AKK IYNVSCERY+GFGCEIDE TSNKL GLPGV FVL
Sbjct: 46 TKQQMIDCYIQTLAKVLGSEEEAKKKIYNVSCERYFGFGCEIDEETSNKLEGLPGVLFVL 105
Query: 181 PDSYVDPEYKDYGGELLVDGKIVPRTPERQRRVTPAPQR 297
PDSYVDPEYKDYG EL V+G+IV R+PERQRRV P PQR
Sbjct: 106 PDSYVDPEYKDYGAELFVNGEIVQRSPERQRRVEPVPQR 144
>dbj|BAC22214.1| putative plastid protein [Oryza sativa Japonica Group]
Length = 227
Score = 167 bits (424), Expect = 1e-39
Identities = 79/99 (79%), Positives = 88/99 (88%)
Frame = +1
Query: 1 TKEEMIDHYVKTLARVLGSEEAAKKAIYNVSCERYYGFGCEIDENTSNKLVGLPGVQFVL 180
TK++MID Y++TLA+VLGSEE AKK IYNVSCERY+GFGCEIDE TSNKL GLPGV FVL
Sbjct: 108 TKQQMIDCYIQTLAKVLGSEEEAKKKIYNVSCERYFGFGCEIDEETSNKLEGLPGVLFVL 167
Query: 181 PDSYVDPEYKDYGGELLVDGKIVPRTPERQRRVTPAPQR 297
PDSYVDPEYKDYG EL V+G+IV R+PERQRRV P PQR
Sbjct: 168 PDSYVDPEYKDYGAELFVNGEIVQRSPERQRRVEPVPQR 206
>ref|XP_006656573.1| PREDICTED: DAG protein, chloroplastic-like, partial [Oryza
brachyantha]
Length = 166
Score = 165 bits (418), Expect = 5e-39
Identities = 77/99 (77%), Positives = 88/99 (88%)
Frame = +1
Query: 1 TKEEMIDHYVKTLARVLGSEEAAKKAIYNVSCERYYGFGCEIDENTSNKLVGLPGVQFVL 180
TK++MID Y++TLA+VLGSEE AKK IYNVSCERY+GFGCEIDE TSNKL GLPGV FVL
Sbjct: 47 TKQQMIDCYIQTLAKVLGSEEEAKKKIYNVSCERYFGFGCEIDEETSNKLEGLPGVLFVL 106
Query: 181 PDSYVDPEYKDYGGELLVDGKIVPRTPERQRRVTPAPQR 297
PDSYVDPE+KDYG EL V+G+I+ R+PERQRRV P PQR
Sbjct: 107 PDSYVDPEHKDYGAELFVNGEIIQRSPERQRRVEPVPQR 145
>ref|XP_002312591.2| plastid developmental protein DAG [Populus trichocarpa]
gi|550333256|gb|EEE89958.2| plastid developmental
protein DAG [Populus trichocarpa]
Length = 241
Score = 165 bits (417), Expect = 7e-39
Identities = 78/101 (77%), Positives = 87/101 (86%)
Frame = +1
Query: 1 TKEEMIDHYVKTLARVLGSEEAAKKAIYNVSCERYYGFGCEIDENTSNKLVGLPGVQFVL 180
TK++MID Y++TLA+V+GSEE AK IYNVSCERY+GFGCEIDE TSNKL GLPGV FVL
Sbjct: 122 TKQQMIDCYIETLAKVVGSEEEAKTKIYNVSCERYFGFGCEIDEETSNKLEGLPGVLFVL 181
Query: 181 PDSYVDPEYKDYGGELLVDGKIVPRTPERQRRVTPAPQRIN 303
PDSYVDPEYKDYG EL V+G+IV R PERQRRV P PQR N
Sbjct: 182 PDSYVDPEYKDYGAELFVNGEIVQRPPERQRRVEPQPQRAN 222
>ref|XP_002334594.1| predicted protein [Populus trichocarpa]
Length = 145
Score = 165 bits (417), Expect = 7e-39
Identities = 78/101 (77%), Positives = 87/101 (86%)
Frame = +1
Query: 1 TKEEMIDHYVKTLARVLGSEEAAKKAIYNVSCERYYGFGCEIDENTSNKLVGLPGVQFVL 180
TK++MID Y++TLA+V+GSEE AK IYNVSCERY+GFGCEIDE TSNKL GLPGV FVL
Sbjct: 26 TKQQMIDCYIETLAKVVGSEEEAKTKIYNVSCERYFGFGCEIDEETSNKLEGLPGVLFVL 85
Query: 181 PDSYVDPEYKDYGGELLVDGKIVPRTPERQRRVTPAPQRIN 303
PDSYVDPEYKDYG EL V+G+IV R PERQRRV P PQR N
Sbjct: 86 PDSYVDPEYKDYGAELFVNGEIVQRPPERQRRVEPQPQRAN 126
>gb|EMJ24941.1| hypothetical protein PRUPE_ppa010500mg [Prunus persica]
Length = 247
Score = 164 bits (416), Expect = 9e-39
Identities = 77/99 (77%), Positives = 87/99 (87%)
Frame = +1
Query: 1 TKEEMIDHYVKTLARVLGSEEAAKKAIYNVSCERYYGFGCEIDENTSNKLVGLPGVQFVL 180
TK++MID YV+TLA++LGSEE AKK IYNVSCERY+GFGCEIDE TSNKL G+PGV FVL
Sbjct: 128 TKQQMIDCYVQTLAKILGSEEEAKKKIYNVSCERYFGFGCEIDEETSNKLEGMPGVLFVL 187
Query: 181 PDSYVDPEYKDYGGELLVDGKIVPRTPERQRRVTPAPQR 297
PDSYVDPEYKDYG EL V+G+IV R+PERQRRV P P R
Sbjct: 188 PDSYVDPEYKDYGAELFVNGEIVQRSPERQRRVEPQPSR 226
>ref|XP_002532881.1| DAG protein, chloroplast precursor, putative [Ricinus communis]
gi|223527366|gb|EEF29510.1| DAG protein, chloroplast
precursor, putative [Ricinus communis]
Length = 248
Score = 164 bits (416), Expect = 9e-39
Identities = 78/101 (77%), Positives = 88/101 (87%)
Frame = +1
Query: 1 TKEEMIDHYVKTLARVLGSEEAAKKAIYNVSCERYYGFGCEIDENTSNKLVGLPGVQFVL 180
TK++MID Y++TLA+V+GSEE AKK IYNVSCERY+GFGCEIDE TSNKL GLPGV FVL
Sbjct: 129 TKQQMIDCYIQTLAKVVGSEEEAKKKIYNVSCERYFGFGCEIDEETSNKLEGLPGVLFVL 188
Query: 181 PDSYVDPEYKDYGGELLVDGKIVPRTPERQRRVTPAPQRIN 303
PDSYVDPE KDYG EL V+G+IV R+PERQRRV P PQR N
Sbjct: 189 PDSYVDPENKDYGAELFVNGEIVQRSPERQRRVEPQPQRAN 229
>ref|NP_001150208.1| DAG protein [Zea mays] gi|195637572|gb|ACG38254.1| DAG protein [Zea
mays] gi|223947219|gb|ACN27693.1| unknown [Zea mays]
gi|413953464|gb|AFW86113.1| DAG protein [Zea mays]
Length = 223
Score = 164 bits (416), Expect = 9e-39
Identities = 78/99 (78%), Positives = 87/99 (87%)
Frame = +1
Query: 1 TKEEMIDHYVKTLARVLGSEEAAKKAIYNVSCERYYGFGCEIDENTSNKLVGLPGVQFVL 180
+K++MID Y++TLA+VLGSEE AKK IYNVSCERY+GFGCEIDE TSNKL GLPGV FVL
Sbjct: 104 SKQQMIDCYIQTLAKVLGSEEEAKKKIYNVSCERYFGFGCEIDEETSNKLEGLPGVLFVL 163
Query: 181 PDSYVDPEYKDYGGELLVDGKIVPRTPERQRRVTPAPQR 297
PDSYVD EYKDYG EL V+G+IV RTPERQRRV P PQR
Sbjct: 164 PDSYVDAEYKDYGAELFVNGEIVQRTPERQRRVEPVPQR 202
>ref|XP_004965835.1| PREDICTED: uncharacterized protein At3g15000, mitochondrial-like
[Setaria italica]
Length = 230
Score = 164 bits (415), Expect = 1e-38
Identities = 78/98 (79%), Positives = 86/98 (87%)
Frame = +1
Query: 4 KEEMIDHYVKTLARVLGSEEAAKKAIYNVSCERYYGFGCEIDENTSNKLVGLPGVQFVLP 183
K++MID YV+TLA+VLGSEE AK+ IYNVSCERY+GFGCEIDE TSNKL GLPGV FVLP
Sbjct: 112 KQQMIDCYVQTLAKVLGSEEEAKRKIYNVSCERYFGFGCEIDEETSNKLEGLPGVLFVLP 171
Query: 184 DSYVDPEYKDYGGELLVDGKIVPRTPERQRRVTPAPQR 297
DSYVDPEYKDYG EL V+G+IV R PERQRRV P PQR
Sbjct: 172 DSYVDPEYKDYGAELFVNGEIVQRPPERQRRVEPVPQR 209
>ref|NP_001236832.1| uncharacterized protein LOC100306054 [Glycine max]
gi|255627403|gb|ACU14046.1| unknown [Glycine max]
Length = 241
Score = 163 bits (413), Expect(2) = 1e-38
Identities = 78/102 (76%), Positives = 88/102 (86%)
Frame = +1
Query: 4 KEEMIDHYVKTLARVLGSEEAAKKAIYNVSCERYYGFGCEIDENTSNKLVGLPGVQFVLP 183
K++MID Y++TLA+VLGSEE AKK IYNVSCERY+GFGCEIDE TSNKL GLPGV FVLP
Sbjct: 119 KQQMIDCYIQTLAKVLGSEEEAKKKIYNVSCERYFGFGCEIDEETSNKLEGLPGVLFVLP 178
Query: 184 DSYVDPEYKDYGGELLVDGKIVPRTPERQRRVTPAPQRINSD 309
DSYVDPE KDYG EL V+G+IV R+PERQRRV P PQR +D
Sbjct: 179 DSYVDPENKDYGAELFVNGEIVQRSPERQRRVEPQPQRHQTD 220
Score = 21.9 bits (45), Expect(2) = 1e-38
Identities = 9/13 (69%), Positives = 10/13 (76%)
Frame = +2
Query: 317 DTMTEQDMSGEER 355
DTM EQ MSG E+
Sbjct: 222 DTMIEQGMSGAEK 234
>ref|XP_006345901.1| PREDICTED: DAG protein, chloroplastic-like [Solanum tuberosum]
Length = 234
Score = 164 bits (414), Expect = 1e-38
Identities = 76/98 (77%), Positives = 87/98 (88%)
Frame = +1
Query: 4 KEEMIDHYVKTLARVLGSEEAAKKAIYNVSCERYYGFGCEIDENTSNKLVGLPGVQFVLP 183
K++MID YV+TLA+VLGSEE AKK IYNVSCERY+GFGCE+DE T+NK+ GLPGV FVLP
Sbjct: 120 KQQMIDCYVQTLAKVLGSEEEAKKKIYNVSCERYFGFGCEVDEETANKMEGLPGVLFVLP 179
Query: 184 DSYVDPEYKDYGGELLVDGKIVPRTPERQRRVTPAPQR 297
DSYVDPEYKDYG EL V+G+IV R+PERQRRV P PQR
Sbjct: 180 DSYVDPEYKDYGAELFVNGEIVQRSPERQRRVEPVPQR 217
>ref|XP_004964371.1| PREDICTED: uncharacterized protein At3g15000, mitochondrial-like
[Setaria italica]
Length = 226
Score = 164 bits (414), Expect = 1e-38
Identities = 77/98 (78%), Positives = 86/98 (87%)
Frame = +1
Query: 4 KEEMIDHYVKTLARVLGSEEAAKKAIYNVSCERYYGFGCEIDENTSNKLVGLPGVQFVLP 183
K++MID Y++TLA+VLGSEE AK+ IYNVSCERY+GFGCEIDE TSNKL GLPGV FVLP
Sbjct: 108 KQQMIDCYIQTLAKVLGSEEEAKRKIYNVSCERYFGFGCEIDEETSNKLEGLPGVLFVLP 167
Query: 184 DSYVDPEYKDYGGELLVDGKIVPRTPERQRRVTPAPQR 297
DSYVDPEYKDYG EL V+G+IV R PERQRRV P PQR
Sbjct: 168 DSYVDPEYKDYGAELFVNGEIVQRPPERQRRVEPVPQR 205
>dbj|BAJ87670.1| predicted protein [Hordeum vulgare subsp. vulgare]
Length = 236
Score = 164 bits (414), Expect = 1e-38
Identities = 76/99 (76%), Positives = 88/99 (88%)
Frame = +1
Query: 1 TKEEMIDHYVKTLARVLGSEEAAKKAIYNVSCERYYGFGCEIDENTSNKLVGLPGVQFVL 180
TK++MID Y++TLA+VLGSEE A+K IYNVSCERY+GFGCEIDE TSNKL G+PGV FVL
Sbjct: 116 TKQQMIDCYIQTLAKVLGSEEEARKKIYNVSCERYFGFGCEIDEETSNKLEGIPGVLFVL 175
Query: 181 PDSYVDPEYKDYGGELLVDGKIVPRTPERQRRVTPAPQR 297
PDSYVDPE+KDYG EL V+G+IV R+PERQRRV P PQR
Sbjct: 176 PDSYVDPEHKDYGAELFVNGEIVQRSPERQRRVEPVPQR 214
>ref|XP_002436376.1| hypothetical protein SORBIDRAFT_10g001390 [Sorghum bicolor]
gi|241914599|gb|EER87743.1| hypothetical protein
SORBIDRAFT_10g001390 [Sorghum bicolor]
Length = 222
Score = 164 bits (414), Expect = 1e-38
Identities = 77/98 (78%), Positives = 86/98 (87%)
Frame = +1
Query: 4 KEEMIDHYVKTLARVLGSEEAAKKAIYNVSCERYYGFGCEIDENTSNKLVGLPGVQFVLP 183
K++MID Y++TLA+VLGSEE AK+ IYNVSCERY+GFGCEIDE TSNKL GLPGV FVLP
Sbjct: 104 KQQMIDCYIQTLAKVLGSEEEAKRKIYNVSCERYFGFGCEIDEETSNKLEGLPGVLFVLP 163
Query: 184 DSYVDPEYKDYGGELLVDGKIVPRTPERQRRVTPAPQR 297
DSYVDPEYKDYG EL V+G+IV R PERQRRV P PQR
Sbjct: 164 DSYVDPEYKDYGAELFVNGEIVQRPPERQRRVEPVPQR 201
>gb|EPS66780.1| hypothetical protein M569_07993 [Genlisea aurea]
Length = 242
Score = 163 bits (413), Expect = 2e-38
Identities = 79/99 (79%), Positives = 86/99 (86%)
Frame = +1
Query: 1 TKEEMIDHYVKTLARVLGSEEAAKKAIYNVSCERYYGFGCEIDENTSNKLVGLPGVQFVL 180
TKE+MID Y+KTLA+VLGSEE AKK IYNVSCERY+GFGCEIDE TSNKL GLPGV FVL
Sbjct: 123 TKEQMIDCYIKTLAQVLGSEEEAKKKIYNVSCERYFGFGCEIDEETSNKLEGLPGVLFVL 182
Query: 181 PDSYVDPEYKDYGGELLVDGKIVPRTPERQRRVTPAPQR 297
PDSYVD E KDYG EL V+G+IV R+PERQRRV P PQR
Sbjct: 183 PDSYVDAENKDYGAELFVNGEIVQRSPERQRRVEPVPQR 221