BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Ephedra27_contig00014569
         (782 letters)

Database: ./nr 
           37,332,560 sequences; 13,225,080,153 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ABR18369.1| unknown [Picea sitchensis]                             456   e-126
ref|NP_001045279.1| Os01g0928800 [Oryza sativa Japonica Group] g...   437   e-120
gb|EOY02914.1| Long chain base2 isoform 2 [Theobroma cacao]           436   e-120
gb|EOY02913.1| Long chain base2 isoform 1 [Theobroma cacao]           436   e-120
ref|XP_002318160.1| serine palmitoyltransferase family protein [...   435   e-119
ref|XP_006645277.1| PREDICTED: long chain base biosynthesis prot...   434   e-119
ref|XP_002450805.1| hypothetical protein SORBIDRAFT_05g018880 [S...   434   e-119
dbj|BAC55228.1| serine palmitoyltransferase [Lotus japonicus]         434   e-119
ref|XP_004979342.1| PREDICTED: long chain base biosynthesis prot...   433   e-119
gb|EOY22597.1| Long chain base2 isoform 2 [Theobroma cacao]           433   e-119
ref|XP_003518128.1| PREDICTED: long chain base biosynthesis prot...   432   e-119
ref|XP_006662956.1| PREDICTED: long chain base biosynthesis prot...   431   e-118
ref|NP_001067972.1| Os11g0516000 [Oryza sativa Japonica Group] g...   431   e-118
ref|XP_002459025.1| hypothetical protein SORBIDRAFT_03g044700 [S...   431   e-118
gb|EEE52176.1| hypothetical protein OsJ_34042 [Oryza sativa Japo...   431   e-118
ref|NP_001151995.1| LOC100285632 [Zea mays] gi|195651679|gb|ACG4...   431   e-118
ref|XP_004138028.1| PREDICTED: long chain base biosynthesis prot...   431   e-118
ref|XP_003548027.1| PREDICTED: long chain base biosynthesis prot...   431   e-118
gb|ACG26228.1| serine palmitoyltransferase 2 [Zea mays] gi|21988...   430   e-118
ref|NP_001148953.1| serine palmitoyltransferase 2 [Zea mays] gi|...   430   e-118

>gb|ABR18369.1| unknown [Picea sitchensis]
          Length = 493

 Score =  456 bits (1174), Expect = e-126
 Identities = 224/260 (86%), Positives = 239/260 (91%)
 Frame = +1

Query: 1   HLEEVLRESIAEGQPRTHRPWKKIIVIVEGIYSMEGELCKLPEIVAVCKKYKAYTYLDEA 180
           HLEEVLRE+IA+GQPRTHRPWKKIIV+VEGIYSMEGELCKLPEIVAVC KYKAY YLDEA
Sbjct: 218 HLEEVLREAIADGQPRTHRPWKKIIVVVEGIYSMEGELCKLPEIVAVCNKYKAYLYLDEA 277

Query: 181 HSIGAVGKSGRGICELLGVDPADIDIMMGTFTKSFGSCGGYIAGSTDLIKYLKFVSPGHL 360
           HSIGA+GK+GRGICELLGVD +DIDIMMGTFTKSFGSCGGYIAGS DLIKYLK VSPGHL
Sbjct: 278 HSIGAIGKTGRGICELLGVDTSDIDIMMGTFTKSFGSCGGYIAGSKDLIKYLKAVSPGHL 337

Query: 361 YATSMSPPCVQQVISAIKVILGEDGSNRGAQKLARIRENSNFFRAELQKMGCEVLGDHDS 540
           YAT+MSPP VQQVISA+KVILGEDGSNRGAQKLARIRENSNFFRAELQKMGCEVLGDHDS
Sbjct: 338 YATAMSPPAVQQVISALKVILGEDGSNRGAQKLARIRENSNFFRAELQKMGCEVLGDHDS 397

Query: 541 PVMPIMLYNPAKIPAFSRECLRRHXXXXXXXXXXXXLLLARARICISASHSKEDLMKALE 720
           PVMPIMLYNPAKIPAFSRECL+RH            LLLARAR+C+SASH+KEDL+KALE
Sbjct: 398 PVMPIMLYNPAKIPAFSRECLKRHVAVVGVAFPATPLLLARARMCLSASHTKEDLLKALE 457

Query: 721 VISEVGDMVHIKYFPRELEK 780
           VISEV DMV IKYFP++ EK
Sbjct: 458 VISEVSDMVQIKYFPKDPEK 477


>ref|NP_001045279.1| Os01g0928800 [Oryza sativa Japonica Group]
           gi|75105817|sp|Q5JK39.1|LCB2D_ORYSJ RecName: Full=Long
           chain base biosynthesis protein 2d
           gi|57900106|dbj|BAD88168.1| putative serine
           palmitoyltransferase [Oryza sativa Japonica Group]
           gi|113534810|dbj|BAF07193.1| Os01g0928800 [Oryza sativa
           Japonica Group] gi|215767866|dbj|BAH00095.1| unnamed
           protein product [Oryza sativa Japonica Group]
           gi|218189664|gb|EEC72091.1| hypothetical protein
           OsI_05038 [Oryza sativa Indica Group]
           gi|222619808|gb|EEE55940.1| hypothetical protein
           OsJ_04635 [Oryza sativa Japonica Group]
          Length = 489

 Score =  437 bits (1123), Expect = e-120
 Identities = 215/260 (82%), Positives = 234/260 (90%)
 Frame = +1

Query: 1   HLEEVLRESIAEGQPRTHRPWKKIIVIVEGIYSMEGELCKLPEIVAVCKKYKAYTYLDEA 180
           HLEEVLRE IA GQPRTHRPWKKIIVIVEGIYSMEGELCKLPE++AVCKKYKAYTYLDEA
Sbjct: 219 HLEEVLREQIAGGQPRTHRPWKKIIVIVEGIYSMEGELCKLPEVIAVCKKYKAYTYLDEA 278

Query: 181 HSIGAVGKSGRGICELLGVDPADIDIMMGTFTKSFGSCGGYIAGSTDLIKYLKFVSPGHL 360
           HSIGAVGK+GRG+CELLGVDPAD+DIMMGTFTKSFGSCGGYIA S ++I +LK + P H+
Sbjct: 279 HSIGAVGKTGRGVCELLGVDPADVDIMMGTFTKSFGSCGGYIAASKEIIDHLKHICPAHI 338

Query: 361 YATSMSPPCVQQVISAIKVILGEDGSNRGAQKLARIRENSNFFRAELQKMGCEVLGDHDS 540
           YATSMSPP VQQVISAIKVILGEDGSNRGA+KLA+IRENSNFFR+ELQKMG EVLGD+DS
Sbjct: 339 YATSMSPPAVQQVISAIKVILGEDGSNRGAKKLAQIRENSNFFRSELQKMGFEVLGDNDS 398

Query: 541 PVMPIMLYNPAKIPAFSRECLRRHXXXXXXXXXXXXLLLARARICISASHSKEDLMKALE 720
           PVMPIMLYNPAKIPAFSRECLR+H            LLLARARICISASHS+EDL+K LE
Sbjct: 399 PVMPIMLYNPAKIPAFSRECLRQHVAVVTVAFPATPLLLARARICISASHSREDLIKGLE 458

Query: 721 VISEVGDMVHIKYFPRELEK 780
           VIS+VGD+V IKYFP E EK
Sbjct: 459 VISKVGDLVGIKYFPVEHEK 478


>gb|EOY02914.1| Long chain base2 isoform 2 [Theobroma cacao]
          Length = 454

 Score =  436 bits (1120), Expect = e-120
 Identities = 212/260 (81%), Positives = 235/260 (90%)
 Frame = +1

Query: 1   HLEEVLRESIAEGQPRTHRPWKKIIVIVEGIYSMEGELCKLPEIVAVCKKYKAYTYLDEA 180
           HLEEVLRE IA+GQPRTHRPWKKI+VIVEGIYSMEGELCKLPEI+A+CKKYKAYTYLDEA
Sbjct: 185 HLEEVLREQIADGQPRTHRPWKKIVVIVEGIYSMEGELCKLPEIIAICKKYKAYTYLDEA 244

Query: 181 HSIGAVGKSGRGICELLGVDPADIDIMMGTFTKSFGSCGGYIAGSTDLIKYLKFVSPGHL 360
           HSIGAVGK+GRG+CELLGVDPAD+DIMMGTFTKSFGSCGGYIAGS +LI+YLK+  P HL
Sbjct: 245 HSIGAVGKTGRGVCELLGVDPADVDIMMGTFTKSFGSCGGYIAGSKELIQYLKYTCPAHL 304

Query: 361 YATSMSPPCVQQVISAIKVILGEDGSNRGAQKLARIRENSNFFRAELQKMGCEVLGDHDS 540
           YATS+SPP  QQ+IS+IKVILG+DGSNRGAQKLA+IRENSNFFR+EL KMG EVLGD+DS
Sbjct: 305 YATSISPPAAQQIISSIKVILGQDGSNRGAQKLAQIRENSNFFRSELIKMGFEVLGDNDS 364

Query: 541 PVMPIMLYNPAKIPAFSRECLRRHXXXXXXXXXXXXLLLARARICISASHSKEDLMKALE 720
           PVMPIMLYNPAKIPAFSRECL+++            LLLARARICISASH+KEDL+KALE
Sbjct: 365 PVMPIMLYNPAKIPAFSRECLKQNVAVVTVAFPATPLLLARARICISASHTKEDLIKALE 424

Query: 721 VISEVGDMVHIKYFPRELEK 780
           VIS VGD+V IKYFP E EK
Sbjct: 425 VISRVGDLVGIKYFPAEPEK 444


>gb|EOY02913.1| Long chain base2 isoform 1 [Theobroma cacao]
          Length = 489

 Score =  436 bits (1120), Expect = e-120
 Identities = 212/260 (81%), Positives = 235/260 (90%)
 Frame = +1

Query: 1   HLEEVLRESIAEGQPRTHRPWKKIIVIVEGIYSMEGELCKLPEIVAVCKKYKAYTYLDEA 180
           HLEEVLRE IA+GQPRTHRPWKKI+VIVEGIYSMEGELCKLPEI+A+CKKYKAYTYLDEA
Sbjct: 220 HLEEVLREQIADGQPRTHRPWKKIVVIVEGIYSMEGELCKLPEIIAICKKYKAYTYLDEA 279

Query: 181 HSIGAVGKSGRGICELLGVDPADIDIMMGTFTKSFGSCGGYIAGSTDLIKYLKFVSPGHL 360
           HSIGAVGK+GRG+CELLGVDPAD+DIMMGTFTKSFGSCGGYIAGS +LI+YLK+  P HL
Sbjct: 280 HSIGAVGKTGRGVCELLGVDPADVDIMMGTFTKSFGSCGGYIAGSKELIQYLKYTCPAHL 339

Query: 361 YATSMSPPCVQQVISAIKVILGEDGSNRGAQKLARIRENSNFFRAELQKMGCEVLGDHDS 540
           YATS+SPP  QQ+IS+IKVILG+DGSNRGAQKLA+IRENSNFFR+EL KMG EVLGD+DS
Sbjct: 340 YATSISPPAAQQIISSIKVILGQDGSNRGAQKLAQIRENSNFFRSELIKMGFEVLGDNDS 399

Query: 541 PVMPIMLYNPAKIPAFSRECLRRHXXXXXXXXXXXXLLLARARICISASHSKEDLMKALE 720
           PVMPIMLYNPAKIPAFSRECL+++            LLLARARICISASH+KEDL+KALE
Sbjct: 400 PVMPIMLYNPAKIPAFSRECLKQNVAVVTVAFPATPLLLARARICISASHTKEDLIKALE 459

Query: 721 VISEVGDMVHIKYFPRELEK 780
           VIS VGD+V IKYFP E EK
Sbjct: 460 VISRVGDLVGIKYFPAEPEK 479


>ref|XP_002318160.1| serine palmitoyltransferase family protein [Populus trichocarpa]
           gi|222858833|gb|EEE96380.1| serine palmitoyltransferase
           family protein [Populus trichocarpa]
          Length = 489

 Score =  435 bits (1118), Expect = e-119
 Identities = 214/260 (82%), Positives = 235/260 (90%)
 Frame = +1

Query: 1   HLEEVLRESIAEGQPRTHRPWKKIIVIVEGIYSMEGELCKLPEIVAVCKKYKAYTYLDEA 180
           HLEEVLR+ IAEGQPRTHRPWKKIIVIVEGIYSMEGELCKLPEIVAVCKKYKAY YLDEA
Sbjct: 219 HLEEVLRQQIAEGQPRTHRPWKKIIVIVEGIYSMEGELCKLPEIVAVCKKYKAYVYLDEA 278

Query: 181 HSIGAVGKSGRGICELLGVDPADIDIMMGTFTKSFGSCGGYIAGSTDLIKYLKFVSPGHL 360
           HSIGAVGK+GRG+CELLGVD AD+DIMMGTFTKSFGSCGGYIAGS +LI+YLK+  P HL
Sbjct: 279 HSIGAVGKTGRGVCELLGVDTADVDIMMGTFTKSFGSCGGYIAGSKELIQYLKYNCPAHL 338

Query: 361 YATSMSPPCVQQVISAIKVILGEDGSNRGAQKLARIRENSNFFRAELQKMGCEVLGDHDS 540
           YATS+SPP  QQ+IS+IKVILGEDGS+RGAQKLARIRENSNFFR+ELQKMG EVLGD+DS
Sbjct: 339 YATSISPPAAQQIISSIKVILGEDGSSRGAQKLARIRENSNFFRSELQKMGFEVLGDNDS 398

Query: 541 PVMPIMLYNPAKIPAFSRECLRRHXXXXXXXXXXXXLLLARARICISASHSKEDLMKALE 720
           PVMPIMLYNPAKIPAFSRECL+++            LLLARARICISASH+KEDL+KALE
Sbjct: 399 PVMPIMLYNPAKIPAFSRECLKQNVAVVTVAFPATPLLLARARICISASHTKEDLLKALE 458

Query: 721 VISEVGDMVHIKYFPRELEK 780
           VIS+VGD+V IKYFP E +K
Sbjct: 459 VISQVGDLVGIKYFPAESDK 478


>ref|XP_006645277.1| PREDICTED: long chain base biosynthesis protein 2d-like [Oryza
           brachyantha]
          Length = 489

 Score =  434 bits (1116), Expect = e-119
 Identities = 214/260 (82%), Positives = 232/260 (89%)
 Frame = +1

Query: 1   HLEEVLRESIAEGQPRTHRPWKKIIVIVEGIYSMEGELCKLPEIVAVCKKYKAYTYLDEA 180
           HLEEVLRE IA GQPRTHRPWKKIIVIVEGIYSMEGELCKLPEI+AVCKKYK YTYLDEA
Sbjct: 219 HLEEVLREQIAGGQPRTHRPWKKIIVIVEGIYSMEGELCKLPEIIAVCKKYKVYTYLDEA 278

Query: 181 HSIGAVGKSGRGICELLGVDPADIDIMMGTFTKSFGSCGGYIAGSTDLIKYLKFVSPGHL 360
           HSIGAVGK+GRG+CELLGVDP D+DIMMGTFTKSFGSCGGYIA S ++I +LK + P H+
Sbjct: 279 HSIGAVGKTGRGVCELLGVDPTDVDIMMGTFTKSFGSCGGYIAASKEIIDHLKHICPAHI 338

Query: 361 YATSMSPPCVQQVISAIKVILGEDGSNRGAQKLARIRENSNFFRAELQKMGCEVLGDHDS 540
           YATSMSPP VQQVISAIKVILGEDGSNRGA+KLA+IRENSNFFR+ELQKMG EVLGD+DS
Sbjct: 339 YATSMSPPAVQQVISAIKVILGEDGSNRGAKKLAQIRENSNFFRSELQKMGFEVLGDNDS 398

Query: 541 PVMPIMLYNPAKIPAFSRECLRRHXXXXXXXXXXXXLLLARARICISASHSKEDLMKALE 720
           PVMPIMLYNPAKIPAFSRECLR+H            LLLARARICISASHS+EDL+K LE
Sbjct: 399 PVMPIMLYNPAKIPAFSRECLRQHVAVVTVAFPATPLLLARARICISASHSREDLIKGLE 458

Query: 721 VISEVGDMVHIKYFPRELEK 780
           VIS+VGD+V IKYFP E EK
Sbjct: 459 VISKVGDLVGIKYFPVEHEK 478


>ref|XP_002450805.1| hypothetical protein SORBIDRAFT_05g018880 [Sorghum bicolor]
           gi|241936648|gb|EES09793.1| hypothetical protein
           SORBIDRAFT_05g018880 [Sorghum bicolor]
          Length = 488

 Score =  434 bits (1116), Expect = e-119
 Identities = 217/260 (83%), Positives = 232/260 (89%)
 Frame = +1

Query: 1   HLEEVLRESIAEGQPRTHRPWKKIIVIVEGIYSMEGELCKLPEIVAVCKKYKAYTYLDEA 180
           HLEEVLRE IA GQPRTHRPWKKIIVIVEGIYSMEGELCKLPEI+AVCKKYKAYTYLDEA
Sbjct: 219 HLEEVLREQIAGGQPRTHRPWKKIIVIVEGIYSMEGELCKLPEIIAVCKKYKAYTYLDEA 278

Query: 181 HSIGAVGKSGRGICELLGVDPADIDIMMGTFTKSFGSCGGYIAGSTDLIKYLKFVSPGHL 360
           HSIGAVG+SGRG+CELLGVDPAD+DIMMGTFTKSFGSCGGYIA S ++I++LK   P HL
Sbjct: 279 HSIGAVGQSGRGVCELLGVDPADVDIMMGTFTKSFGSCGGYIAASKEIIQHLKQSCPAHL 338

Query: 361 YATSMSPPCVQQVISAIKVILGEDGSNRGAQKLARIRENSNFFRAELQKMGCEVLGDHDS 540
           YATSMSPP VQQVISAIKVILGEDGSNRGAQKLARIRENSNFFR+EL+KMG EVLGD+DS
Sbjct: 339 YATSMSPPAVQQVISAIKVILGEDGSNRGAQKLARIRENSNFFRSELKKMGFEVLGDNDS 398

Query: 541 PVMPIMLYNPAKIPAFSRECLRRHXXXXXXXXXXXXLLLARARICISASHSKEDLMKALE 720
           PVMPIMLYNPAKIPAFSRECLRR             LLLARARICISASH++EDLMKAL+
Sbjct: 399 PVMPIMLYNPAKIPAFSRECLRRKVAVVTVAFPATPLLLARARICISASHTREDLMKALD 458

Query: 721 VISEVGDMVHIKYFPRELEK 780
           VIS VGD+V IKYFP E  K
Sbjct: 459 VISRVGDLVGIKYFPAEPPK 478


>dbj|BAC55228.1| serine palmitoyltransferase [Lotus japonicus]
          Length = 489

 Score =  434 bits (1115), Expect = e-119
 Identities = 213/260 (81%), Positives = 233/260 (89%)
 Frame = +1

Query: 1   HLEEVLRESIAEGQPRTHRPWKKIIVIVEGIYSMEGELCKLPEIVAVCKKYKAYTYLDEA 180
           HLEEVLRE IAEGQPRTHRPWKKI+VIVEGIYSMEGELCKLPEI+A+CKKYKAYTYLDEA
Sbjct: 219 HLEEVLREQIAEGQPRTHRPWKKIMVIVEGIYSMEGELCKLPEIIAICKKYKAYTYLDEA 278

Query: 181 HSIGAVGKSGRGICELLGVDPADIDIMMGTFTKSFGSCGGYIAGSTDLIKYLKFVSPGHL 360
           HSIGAVGK+GRG+CELLGVD ADIDIMMGTFTKSFGSCGGYIAGS +LI+YLK+  P HL
Sbjct: 279 HSIGAVGKTGRGVCELLGVDTADIDIMMGTFTKSFGSCGGYIAGSQELIQYLKYTCPAHL 338

Query: 361 YATSMSPPCVQQVISAIKVILGEDGSNRGAQKLARIRENSNFFRAELQKMGCEVLGDHDS 540
           YATS+SPP  QQ+IS+IKVILGEDGSNRGAQKLA+IRENSNFFR+ELQKMG EVLGD+DS
Sbjct: 339 YATSVSPPAAQQIISSIKVILGEDGSNRGAQKLAKIRENSNFFRSELQKMGFEVLGDNDS 398

Query: 541 PVMPIMLYNPAKIPAFSRECLRRHXXXXXXXXXXXXLLLARARICISASHSKEDLMKALE 720
           PVMPIMLYNPAKIPAFSRECLR++            LLLARARICISASHS+EDL+ AL+
Sbjct: 399 PVMPIMLYNPAKIPAFSRECLRQNVAVVTVAFPATPLLLARARICISASHSREDLVTALQ 458

Query: 721 VISEVGDMVHIKYFPRELEK 780
           VIS VGD+V IKYFP E  K
Sbjct: 459 VISRVGDLVGIKYFPAEPSK 478


>ref|XP_004979342.1| PREDICTED: long chain base biosynthesis protein 2a-like [Setaria
           italica]
          Length = 488

 Score =  433 bits (1114), Expect = e-119
 Identities = 216/260 (83%), Positives = 232/260 (89%)
 Frame = +1

Query: 1   HLEEVLRESIAEGQPRTHRPWKKIIVIVEGIYSMEGELCKLPEIVAVCKKYKAYTYLDEA 180
           HLEEVLRE IA GQPRTHRPWKKIIVIVEGIYSMEGELCKLPEI+AVCKKYKAYTYLDEA
Sbjct: 219 HLEEVLREQIAGGQPRTHRPWKKIIVIVEGIYSMEGELCKLPEIIAVCKKYKAYTYLDEA 278

Query: 181 HSIGAVGKSGRGICELLGVDPADIDIMMGTFTKSFGSCGGYIAGSTDLIKYLKFVSPGHL 360
           HSIGAVG+SGRG+CELLGVDPAD+DIMMGTFTKSFGSCGGYIA S ++I++LK   P HL
Sbjct: 279 HSIGAVGQSGRGVCELLGVDPADVDIMMGTFTKSFGSCGGYIAASKEIIQHLKHSCPAHL 338

Query: 361 YATSMSPPCVQQVISAIKVILGEDGSNRGAQKLARIRENSNFFRAELQKMGCEVLGDHDS 540
           YATSMSPP VQQVISAIKVILGEDGSNRGAQKLARIRENSNFFR+EL+KMG EVLGD+DS
Sbjct: 339 YATSMSPPAVQQVISAIKVILGEDGSNRGAQKLARIRENSNFFRSELKKMGFEVLGDNDS 398

Query: 541 PVMPIMLYNPAKIPAFSRECLRRHXXXXXXXXXXXXLLLARARICISASHSKEDLMKALE 720
           PVMPIMLYNPAKIPAFSRECLR+             LLLARARICISASH++EDLMKAL+
Sbjct: 399 PVMPIMLYNPAKIPAFSRECLRQKVAVVTVAFPATPLLLARARICISASHTREDLMKALD 458

Query: 721 VISEVGDMVHIKYFPRELEK 780
           VIS VGD+V IKYFP E  K
Sbjct: 459 VISRVGDLVGIKYFPAEPPK 478


>gb|EOY22597.1| Long chain base2 isoform 2 [Theobroma cacao]
          Length = 489

 Score =  433 bits (1114), Expect = e-119
 Identities = 212/260 (81%), Positives = 234/260 (90%)
 Frame = +1

Query: 1   HLEEVLRESIAEGQPRTHRPWKKIIVIVEGIYSMEGELCKLPEIVAVCKKYKAYTYLDEA 180
           HLE+VLRE IAEGQPRTHRPWKKIIV+VEGIYSMEGELCKLPEIVA+CKKYKAY YLDEA
Sbjct: 219 HLEKVLREQIAEGQPRTHRPWKKIIVVVEGIYSMEGELCKLPEIVAICKKYKAYVYLDEA 278

Query: 181 HSIGAVGKSGRGICELLGVDPADIDIMMGTFTKSFGSCGGYIAGSTDLIKYLKFVSPGHL 360
           HSIGAVGK+GRG+CELLGVD AD+DIMMGTFTKSFGSCGGYIAGS +LI+YLK+  P HL
Sbjct: 279 HSIGAVGKTGRGVCELLGVDTADVDIMMGTFTKSFGSCGGYIAGSKELIQYLKYTCPAHL 338

Query: 361 YATSMSPPCVQQVISAIKVILGEDGSNRGAQKLARIRENSNFFRAELQKMGCEVLGDHDS 540
           Y TS+SPP  +Q+ISAIKVILGEDGS+RGAQKLARIRENSNFFR+ELQKMG EVLGD+DS
Sbjct: 339 YTTSISPPAARQIISAIKVILGEDGSSRGAQKLARIRENSNFFRSELQKMGFEVLGDNDS 398

Query: 541 PVMPIMLYNPAKIPAFSRECLRRHXXXXXXXXXXXXLLLARARICISASHSKEDLMKALE 720
           PVMPIMLYNPAKIPAFSRECL+++            LLLARARICISA+H+KEDL+KALE
Sbjct: 399 PVMPIMLYNPAKIPAFSRECLKQNIAVVTVAFPATPLLLARARICISAAHTKEDLLKALE 458

Query: 721 VISEVGDMVHIKYFPRELEK 780
           VISEVGDMV IKYFP E +K
Sbjct: 459 VISEVGDMVGIKYFPAEPKK 478


>ref|XP_003518128.1| PREDICTED: long chain base biosynthesis protein 2a-like [Glycine
           max]
          Length = 489

 Score =  432 bits (1110), Expect = e-119
 Identities = 210/260 (80%), Positives = 234/260 (90%)
 Frame = +1

Query: 1   HLEEVLRESIAEGQPRTHRPWKKIIVIVEGIYSMEGELCKLPEIVAVCKKYKAYTYLDEA 180
           HLEEVLRE IAEGQPRTHRPWKKI+V+VEGIYSMEGELCKLPE++AVCKKYKAYTYLDEA
Sbjct: 219 HLEEVLREQIAEGQPRTHRPWKKIMVVVEGIYSMEGELCKLPEVIAVCKKYKAYTYLDEA 278

Query: 181 HSIGAVGKSGRGICELLGVDPADIDIMMGTFTKSFGSCGGYIAGSTDLIKYLKFVSPGHL 360
           HSIGAVGKSGRG+CELLGVD AD+DIMMGTFTKSFGSCGGYIAGS DLI++LK+  P HL
Sbjct: 279 HSIGAVGKSGRGVCELLGVDTADVDIMMGTFTKSFGSCGGYIAGSKDLIQHLKYACPAHL 338

Query: 361 YATSMSPPCVQQVISAIKVILGEDGSNRGAQKLARIRENSNFFRAELQKMGCEVLGDHDS 540
           YATS+SPP  QQ+IS+I+VILGEDGSNRGAQKLA+IRENSNFFR+ELQKMG EVLGD+DS
Sbjct: 339 YATSISPPAAQQIISSIRVILGEDGSNRGAQKLAQIRENSNFFRSELQKMGFEVLGDNDS 398

Query: 541 PVMPIMLYNPAKIPAFSRECLRRHXXXXXXXXXXXXLLLARARICISASHSKEDLMKALE 720
           PVMPIMLYNPAKIPAFSRECL+++            LLLARARICISA+HS+EDL+KAL+
Sbjct: 399 PVMPIMLYNPAKIPAFSRECLKQNVAVVTVAFPATPLLLARARICISAAHSREDLIKALQ 458

Query: 721 VISEVGDMVHIKYFPRELEK 780
           VIS VGD+V IKYFP E  K
Sbjct: 459 VISRVGDLVGIKYFPAEPTK 478


>ref|XP_006662956.1| PREDICTED: long chain base biosynthesis protein 2a-like [Oryza
           brachyantha]
          Length = 488

 Score =  431 bits (1109), Expect = e-118
 Identities = 215/260 (82%), Positives = 232/260 (89%)
 Frame = +1

Query: 1   HLEEVLRESIAEGQPRTHRPWKKIIVIVEGIYSMEGELCKLPEIVAVCKKYKAYTYLDEA 180
           HLEEVLRE IA GQPRTHRPWKKIIVIVEGIYSMEGELCKLPEI+AVCKKYKAYTYLDEA
Sbjct: 219 HLEEVLREQIAGGQPRTHRPWKKIIVIVEGIYSMEGELCKLPEIIAVCKKYKAYTYLDEA 278

Query: 181 HSIGAVGKSGRGICELLGVDPADIDIMMGTFTKSFGSCGGYIAGSTDLIKYLKFVSPGHL 360
           HSIGAVG+SGRG+CELLGVDPAD+DIMMGTFTKSFGSCGGYIA S ++I++LK   P HL
Sbjct: 279 HSIGAVGQSGRGVCELLGVDPADVDIMMGTFTKSFGSCGGYIAASKEIIQHLKLSCPAHL 338

Query: 361 YATSMSPPCVQQVISAIKVILGEDGSNRGAQKLARIRENSNFFRAELQKMGCEVLGDHDS 540
           YATSMSPP VQQVISAIKVILGEDGSNRGAQKLARIRENSNFFRAEL+KMG EVLG++DS
Sbjct: 339 YATSMSPPAVQQVISAIKVILGEDGSNRGAQKLARIRENSNFFRAELKKMGFEVLGNNDS 398

Query: 541 PVMPIMLYNPAKIPAFSRECLRRHXXXXXXXXXXXXLLLARARICISASHSKEDLMKALE 720
           PVMPIMLYNPAKIPAFSRECLR+             LLLARARICISASH++EDL+KAL+
Sbjct: 399 PVMPIMLYNPAKIPAFSRECLRQKVAVVTVAFPATPLLLARARICISASHTREDLIKALD 458

Query: 721 VISEVGDMVHIKYFPRELEK 780
           VIS VGD+V IKYFP E  K
Sbjct: 459 VISRVGDLVGIKYFPAEPPK 478


>ref|NP_001067972.1| Os11g0516000 [Oryza sativa Japonica Group]
           gi|122207416|sp|Q2R3K3.1|LCB2A_ORYSJ RecName: Full=Long
           chain base biosynthesis protein 2a
           gi|77551135|gb|ABA93932.1| Serine palmitoyltransferase
           2, putative, expressed [Oryza sativa Japonica Group]
           gi|113645194|dbj|BAF28335.1| Os11g0516000 [Oryza sativa
           Japonica Group] gi|218185820|gb|EEC68247.1| hypothetical
           protein OsI_36267 [Oryza sativa Indica Group]
          Length = 488

 Score =  431 bits (1109), Expect = e-118
 Identities = 214/260 (82%), Positives = 232/260 (89%)
 Frame = +1

Query: 1   HLEEVLRESIAEGQPRTHRPWKKIIVIVEGIYSMEGELCKLPEIVAVCKKYKAYTYLDEA 180
           HLEEVLRE IA GQPRTHRPWKKIIVIVEGIYSMEGELCKLPEI+AVCKKYKAYTYLDEA
Sbjct: 219 HLEEVLREQIAGGQPRTHRPWKKIIVIVEGIYSMEGELCKLPEIIAVCKKYKAYTYLDEA 278

Query: 181 HSIGAVGKSGRGICELLGVDPADIDIMMGTFTKSFGSCGGYIAGSTDLIKYLKFVSPGHL 360
           HSIGAVG+SGRG+CELLGVDPAD+DIMMGTFTKSFGSCGGYIA S ++I++LK   P H+
Sbjct: 279 HSIGAVGQSGRGVCELLGVDPADVDIMMGTFTKSFGSCGGYIAASKEIIQHLKLSCPAHI 338

Query: 361 YATSMSPPCVQQVISAIKVILGEDGSNRGAQKLARIRENSNFFRAELQKMGCEVLGDHDS 540
           YATSMSPP VQQVISAIKVILGEDGSNRGAQKLARIRENSNFFR+EL+KMG EVLGD+DS
Sbjct: 339 YATSMSPPAVQQVISAIKVILGEDGSNRGAQKLARIRENSNFFRSELKKMGFEVLGDNDS 398

Query: 541 PVMPIMLYNPAKIPAFSRECLRRHXXXXXXXXXXXXLLLARARICISASHSKEDLMKALE 720
           PVMPIMLYNPAKIPAFSRECLR+             LLLARARICISASH++EDL+KAL+
Sbjct: 399 PVMPIMLYNPAKIPAFSRECLRQKVAVVTVAFPATPLLLARARICISASHTREDLIKALD 458

Query: 721 VISEVGDMVHIKYFPRELEK 780
           VIS VGD+V IKYFP E  K
Sbjct: 459 VISRVGDLVGIKYFPAEPPK 478


>ref|XP_002459025.1| hypothetical protein SORBIDRAFT_03g044700 [Sorghum bicolor]
           gi|241931000|gb|EES04145.1| hypothetical protein
           SORBIDRAFT_03g044700 [Sorghum bicolor]
          Length = 489

 Score =  431 bits (1109), Expect = e-118
 Identities = 211/260 (81%), Positives = 233/260 (89%)
 Frame = +1

Query: 1   HLEEVLRESIAEGQPRTHRPWKKIIVIVEGIYSMEGELCKLPEIVAVCKKYKAYTYLDEA 180
           HLEEVLRE IA GQPRTHRPWKKIIVIVEGIYSMEGELCKLPE+++VCKKYKAYTYLDEA
Sbjct: 219 HLEEVLREQIAGGQPRTHRPWKKIIVIVEGIYSMEGELCKLPEVISVCKKYKAYTYLDEA 278

Query: 181 HSIGAVGKSGRGICELLGVDPADIDIMMGTFTKSFGSCGGYIAGSTDLIKYLKFVSPGHL 360
           HSIGA+GK+GRG+CELLGVDPAD+DIMMGTFTKSFGSCGGYIA S ++I +LK   P H+
Sbjct: 279 HSIGAIGKTGRGVCELLGVDPADVDIMMGTFTKSFGSCGGYIAASNEIIHHLKLTCPAHI 338

Query: 361 YATSMSPPCVQQVISAIKVILGEDGSNRGAQKLARIRENSNFFRAELQKMGCEVLGDHDS 540
           YATSMSPP VQQVISAIKVILGEDG+NRGA+KLA+IRENSNFFR+ELQKMG EVLGD+DS
Sbjct: 339 YATSMSPPAVQQVISAIKVILGEDGTNRGAKKLAQIRENSNFFRSELQKMGFEVLGDNDS 398

Query: 541 PVMPIMLYNPAKIPAFSRECLRRHXXXXXXXXXXXXLLLARARICISASHSKEDLMKALE 720
           PVMPIMLYNPAKIPAFSRECLR++            LLLARARICISASHS+EDL+K LE
Sbjct: 399 PVMPIMLYNPAKIPAFSRECLRQNVAVVTVAFPATPLLLARARICISASHSREDLIKGLE 458

Query: 721 VISEVGDMVHIKYFPRELEK 780
           VIS+VGD+V IKYFP E EK
Sbjct: 459 VISKVGDLVGIKYFPVEQEK 478


>gb|EEE52176.1| hypothetical protein OsJ_34042 [Oryza sativa Japonica Group]
          Length = 466

 Score =  431 bits (1109), Expect = e-118
 Identities = 214/260 (82%), Positives = 232/260 (89%)
 Frame = +1

Query: 1   HLEEVLRESIAEGQPRTHRPWKKIIVIVEGIYSMEGELCKLPEIVAVCKKYKAYTYLDEA 180
           HLEEVLRE IA GQPRTHRPWKKIIVIVEGIYSMEGELCKLPEI+AVCKKYKAYTYLDEA
Sbjct: 197 HLEEVLREQIAGGQPRTHRPWKKIIVIVEGIYSMEGELCKLPEIIAVCKKYKAYTYLDEA 256

Query: 181 HSIGAVGKSGRGICELLGVDPADIDIMMGTFTKSFGSCGGYIAGSTDLIKYLKFVSPGHL 360
           HSIGAVG+SGRG+CELLGVDPAD+DIMMGTFTKSFGSCGGYIA S ++I++LK   P H+
Sbjct: 257 HSIGAVGQSGRGVCELLGVDPADVDIMMGTFTKSFGSCGGYIAASKEIIQHLKLSCPAHI 316

Query: 361 YATSMSPPCVQQVISAIKVILGEDGSNRGAQKLARIRENSNFFRAELQKMGCEVLGDHDS 540
           YATSMSPP VQQVISAIKVILGEDGSNRGAQKLARIRENSNFFR+EL+KMG EVLGD+DS
Sbjct: 317 YATSMSPPAVQQVISAIKVILGEDGSNRGAQKLARIRENSNFFRSELKKMGFEVLGDNDS 376

Query: 541 PVMPIMLYNPAKIPAFSRECLRRHXXXXXXXXXXXXLLLARARICISASHSKEDLMKALE 720
           PVMPIMLYNPAKIPAFSRECLR+             LLLARARICISASH++EDL+KAL+
Sbjct: 377 PVMPIMLYNPAKIPAFSRECLRQKVAVVTVAFPATPLLLARARICISASHTREDLIKALD 436

Query: 721 VISEVGDMVHIKYFPRELEK 780
           VIS VGD+V IKYFP E  K
Sbjct: 437 VISRVGDLVGIKYFPAEPPK 456


>ref|NP_001151995.1| LOC100285632 [Zea mays] gi|195651679|gb|ACG45307.1| serine
           palmitoyltransferase 2 [Zea mays]
           gi|223943295|gb|ACN25731.1| unknown [Zea mays]
           gi|413920924|gb|AFW60856.1| Serine palmitoyltransferase
           2 [Zea mays]
          Length = 488

 Score =  431 bits (1109), Expect = e-118
 Identities = 215/260 (82%), Positives = 231/260 (88%)
 Frame = +1

Query: 1   HLEEVLRESIAEGQPRTHRPWKKIIVIVEGIYSMEGELCKLPEIVAVCKKYKAYTYLDEA 180
           HLEEVLRE IA GQPRTHRPWKKIIVIVEGIYSMEGELCKLPEI+AVCKKYKAYTYLDEA
Sbjct: 219 HLEEVLREQIAGGQPRTHRPWKKIIVIVEGIYSMEGELCKLPEIIAVCKKYKAYTYLDEA 278

Query: 181 HSIGAVGKSGRGICELLGVDPADIDIMMGTFTKSFGSCGGYIAGSTDLIKYLKFVSPGHL 360
           HSIGAVG+SGRG+CELLGVDPAD+DIMMGTFTKSFGSCGGYIA S ++IK+LK   P HL
Sbjct: 279 HSIGAVGQSGRGVCELLGVDPADVDIMMGTFTKSFGSCGGYIAASKEIIKHLKHSCPAHL 338

Query: 361 YATSMSPPCVQQVISAIKVILGEDGSNRGAQKLARIRENSNFFRAELQKMGCEVLGDHDS 540
           YATSMSPP VQQVISAIKVILGEDGS+RGAQKLARIREN NFFR+EL+KMG EVLGD+DS
Sbjct: 339 YATSMSPPAVQQVISAIKVILGEDGSSRGAQKLARIRENGNFFRSELKKMGFEVLGDNDS 398

Query: 541 PVMPIMLYNPAKIPAFSRECLRRHXXXXXXXXXXXXLLLARARICISASHSKEDLMKALE 720
           PVMPIMLYNPAKIPAFSRECLR+             LLLARARICISASH++EDLMKAL+
Sbjct: 399 PVMPIMLYNPAKIPAFSRECLRQKVAVVTVAFPATPLLLARARICISASHTREDLMKALD 458

Query: 721 VISEVGDMVHIKYFPRELEK 780
           VIS VGD+V IKYFP E  K
Sbjct: 459 VISRVGDLVGIKYFPAEPPK 478


>ref|XP_004138028.1| PREDICTED: long chain base biosynthesis protein 2a-like [Cucumis
           sativus]
          Length = 490

 Score =  431 bits (1108), Expect = e-118
 Identities = 209/260 (80%), Positives = 233/260 (89%)
 Frame = +1

Query: 1   HLEEVLRESIAEGQPRTHRPWKKIIVIVEGIYSMEGELCKLPEIVAVCKKYKAYTYLDEA 180
           HL++VLRE IA+GQPRTHRPWKKIIV+VEGIYSMEGE+CKLPEIVA+CKKYKAY YLDEA
Sbjct: 219 HLDKVLREQIADGQPRTHRPWKKIIVVVEGIYSMEGEICKLPEIVAICKKYKAYVYLDEA 278

Query: 181 HSIGAVGKSGRGICELLGVDPADIDIMMGTFTKSFGSCGGYIAGSTDLIKYLKFVSPGHL 360
           HSIGAVGK+GRG+CELLGVD AD+DIMMGTFTKSFGSCGGYIAGS +LIKYLK+  P HL
Sbjct: 279 HSIGAVGKTGRGVCELLGVDTADVDIMMGTFTKSFGSCGGYIAGSKELIKYLKYTCPAHL 338

Query: 361 YATSMSPPCVQQVISAIKVILGEDGSNRGAQKLARIRENSNFFRAELQKMGCEVLGDHDS 540
           YATS+SPP  QQ+IS+IKVILGEDGS+RGAQKLARIRENSNFFR+ELQKMG EVLGD+DS
Sbjct: 339 YATSISPPAAQQIISSIKVILGEDGSSRGAQKLARIRENSNFFRSELQKMGFEVLGDNDS 398

Query: 541 PVMPIMLYNPAKIPAFSRECLRRHXXXXXXXXXXXXLLLARARICISASHSKEDLMKALE 720
           PVMPIMLYNPAKIPAFSRECL ++            LLLARARICISASH+K+DL+KALE
Sbjct: 399 PVMPIMLYNPAKIPAFSRECLNQNVAVVTVAFPATPLLLARARICISASHTKDDLLKALE 458

Query: 721 VISEVGDMVHIKYFPRELEK 780
           VIS VGD+V IKYFP E +K
Sbjct: 459 VISRVGDLVGIKYFPAEAQK 478


>ref|XP_003548027.1| PREDICTED: long chain base biosynthesis protein 2a-like [Glycine
           max]
          Length = 490

 Score =  431 bits (1108), Expect = e-118
 Identities = 210/260 (80%), Positives = 234/260 (90%)
 Frame = +1

Query: 1   HLEEVLRESIAEGQPRTHRPWKKIIVIVEGIYSMEGELCKLPEIVAVCKKYKAYTYLDEA 180
           HLEEVLRE IAEGQPRTHRPWKKI+VIVEGIYSMEGELCKLPE++AVCKKYKAYTYLDEA
Sbjct: 219 HLEEVLREQIAEGQPRTHRPWKKIMVIVEGIYSMEGELCKLPEVIAVCKKYKAYTYLDEA 278

Query: 181 HSIGAVGKSGRGICELLGVDPADIDIMMGTFTKSFGSCGGYIAGSTDLIKYLKFVSPGHL 360
           HSIGAVGK+GRG+CELLGVD AD+DIMMGTFTKSFGSCGGYIAGS DLI++LK+  P HL
Sbjct: 279 HSIGAVGKTGRGVCELLGVDTADVDIMMGTFTKSFGSCGGYIAGSKDLIQHLKYTCPAHL 338

Query: 361 YATSMSPPCVQQVISAIKVILGEDGSNRGAQKLARIRENSNFFRAELQKMGCEVLGDHDS 540
           YATS+SPP  QQ+IS+I+VILGEDGSNRGAQKLA+IRENSNFFR+ELQKMG EVLGD+DS
Sbjct: 339 YATSISPPAAQQIISSIRVILGEDGSNRGAQKLAQIRENSNFFRSELQKMGFEVLGDNDS 398

Query: 541 PVMPIMLYNPAKIPAFSRECLRRHXXXXXXXXXXXXLLLARARICISASHSKEDLMKALE 720
           PVMPIMLYNPAKIPAFSRECL+++            LLLARARICISA+HS+EDL+KAL+
Sbjct: 399 PVMPIMLYNPAKIPAFSRECLKQNVAIVTVAFPATPLLLARARICISAAHSREDLIKALQ 458

Query: 721 VISEVGDMVHIKYFPRELEK 780
           VIS VGD+V IKYFP E  K
Sbjct: 459 VISRVGDLVGIKYFPAEPTK 478


>gb|ACG26228.1| serine palmitoyltransferase 2 [Zea mays]
           gi|219884919|gb|ACL52834.1| unknown [Zea mays]
           gi|413951409|gb|AFW84058.1| putative serine
           palmitoyltransferase [Zea mays]
          Length = 489

 Score =  430 bits (1106), Expect = e-118
 Identities = 212/260 (81%), Positives = 232/260 (89%)
 Frame = +1

Query: 1   HLEEVLRESIAEGQPRTHRPWKKIIVIVEGIYSMEGELCKLPEIVAVCKKYKAYTYLDEA 180
           HLEEVLRE IA GQPRTHRPWKKIIVIVEGIYSMEGELCKLPE+++VCKKYKAYTYLDEA
Sbjct: 219 HLEEVLREQIAGGQPRTHRPWKKIIVIVEGIYSMEGELCKLPEVISVCKKYKAYTYLDEA 278

Query: 181 HSIGAVGKSGRGICELLGVDPADIDIMMGTFTKSFGSCGGYIAGSTDLIKYLKFVSPGHL 360
           HSIGAVGK+GRG+CELLGVDPAD+DIMMGTFTKSFGSCGGYIA S ++I +LK   P H+
Sbjct: 279 HSIGAVGKTGRGVCELLGVDPADVDIMMGTFTKSFGSCGGYIAASNEIIHHLKHTCPAHI 338

Query: 361 YATSMSPPCVQQVISAIKVILGEDGSNRGAQKLARIRENSNFFRAELQKMGCEVLGDHDS 540
           YATSMSPP VQQVISAIKVILGEDG+NRGA+KLA+IRENSNFFR+ELQKMG EVLGD+DS
Sbjct: 339 YATSMSPPAVQQVISAIKVILGEDGTNRGAKKLAQIRENSNFFRSELQKMGFEVLGDNDS 398

Query: 541 PVMPIMLYNPAKIPAFSRECLRRHXXXXXXXXXXXXLLLARARICISASHSKEDLMKALE 720
           PVMPIMLYNPAKIPAFSRECLR+             LLLARARICISASHS+EDL+K LE
Sbjct: 399 PVMPIMLYNPAKIPAFSRECLRQAVAVVTVAFPATPLLLARARICISASHSREDLIKGLE 458

Query: 721 VISEVGDMVHIKYFPRELEK 780
           VIS+VGD+V IKYFP E EK
Sbjct: 459 VISKVGDLVGIKYFPVEQEK 478


>ref|NP_001148953.1| serine palmitoyltransferase 2 [Zea mays]
           gi|195623602|gb|ACG33631.1| serine palmitoyltransferase
           2 [Zea mays] gi|223944845|gb|ACN26506.1| unknown [Zea
           mays] gi|414591435|tpg|DAA42006.1| TPA: Serine
           palmitoyltransferase 2 [Zea mays]
          Length = 488

 Score =  430 bits (1105), Expect = e-118
 Identities = 215/260 (82%), Positives = 231/260 (88%)
 Frame = +1

Query: 1   HLEEVLRESIAEGQPRTHRPWKKIIVIVEGIYSMEGELCKLPEIVAVCKKYKAYTYLDEA 180
           HLEEVLRE IA GQPRTHRPWKKIIVIVEGIYSMEGELCKLPEI+AV KKYKAYTYLDEA
Sbjct: 219 HLEEVLREQIAGGQPRTHRPWKKIIVIVEGIYSMEGELCKLPEIIAVAKKYKAYTYLDEA 278

Query: 181 HSIGAVGKSGRGICELLGVDPADIDIMMGTFTKSFGSCGGYIAGSTDLIKYLKFVSPGHL 360
           HSIGAVG+SGRG+CELLGVDPAD+DIMMGTFTKSFGSCGGYIA S ++I++LK   P HL
Sbjct: 279 HSIGAVGQSGRGVCELLGVDPADVDIMMGTFTKSFGSCGGYIAASKEIIQHLKHSCPAHL 338

Query: 361 YATSMSPPCVQQVISAIKVILGEDGSNRGAQKLARIRENSNFFRAELQKMGCEVLGDHDS 540
           YATSMSPP VQQVISAIKVILGEDGSNRGAQKLARIRENSNFFR+EL+KMG EVLGD+DS
Sbjct: 339 YATSMSPPAVQQVISAIKVILGEDGSNRGAQKLARIRENSNFFRSELKKMGFEVLGDNDS 398

Query: 541 PVMPIMLYNPAKIPAFSRECLRRHXXXXXXXXXXXXLLLARARICISASHSKEDLMKALE 720
           PVMPIMLYNPAKIPAFSRECLR+             LLLARARICISASH++EDLMKAL+
Sbjct: 399 PVMPIMLYNPAKIPAFSRECLRQKVAVVTVAFPATPLLLARARICISASHTREDLMKALD 458

Query: 721 VISEVGDMVHIKYFPRELEK 780
           VIS VGD+V IKYFP E  K
Sbjct: 459 VISRVGDLVGIKYFPAEPPK 478