BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Ephedra27_contig00004837
         (1782 letters)

Database: ./nr 
           37,332,560 sequences; 13,225,080,153 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_004309836.1| PREDICTED: diphthamide biosynthesis protein ...   476   e-131
ref|XP_002273627.2| PREDICTED: diphthamide biosynthesis protein ...   471   e-130
ref|XP_002530564.1| diphteria toxin resistance protein 2, dph2, ...   469   e-129
gb|EXB55202.1| Diphthamide biosynthesis protein 2 [Morus notabilis]   469   e-129
ref|XP_002326199.1| predicted protein [Populus trichocarpa] gi|5...   465   e-128
ref|XP_004152127.1| PREDICTED: diphthamide biosynthesis protein ...   464   e-128
gb|ESW22742.1| hypothetical protein PHAVU_005G177500g [Phaseolus...   463   e-128
ref|XP_004498540.1| PREDICTED: diphthamide biosynthesis protein ...   458   e-126
ref|XP_006478781.1| PREDICTED: diphthamide biosynthesis protein ...   456   e-125
ref|XP_004249376.1| PREDICTED: diphthamide biosynthesis protein ...   456   e-125
ref|XP_003546007.1| PREDICTED: diphthamide biosynthesis protein ...   456   e-125
ref|XP_006597121.1| PREDICTED: diphthamide biosynthesis protein ...   454   e-125
gb|EOY21113.1| Diphthamide synthesis DPH2 family protein [Theobr...   454   e-125
gb|EMJ13032.1| hypothetical protein PRUPE_ppa004543mg [Prunus pe...   453   e-124
ref|XP_006339197.1| PREDICTED: diphthamide biosynthesis protein ...   452   e-124
ref|XP_002878279.1| diphthamide synthesis DPH2 family protein [A...   451   e-124
ref|XP_006397104.1| hypothetical protein EUTSA_v10028620mg [Eutr...   451   e-124
ref|NP_191522.1| diphthamide synthesis DPH2 family protein [Arab...   451   e-124
ref|XP_006290992.1| hypothetical protein CARUB_v10017106mg [Caps...   449   e-123
ref|XP_003588543.1| Diphthamide biosynthesis protein 2 containin...   446   e-122

>ref|XP_004309836.1| PREDICTED: diphthamide biosynthesis protein 2-like [Fragaria vesca
            subsp. vesca]
          Length = 487

 Score =  476 bits (1224), Expect = e-131
 Identities = 250/488 (51%), Positives = 331/488 (67%), Gaps = 13/488 (2%)
 Frame = -1

Query: 1680 ELNALYDVEATATFIHTRGFKRVALQFPDELLKDSVKVVRALKTQLQQNSYDGVQMYVMA 1501
            EL   Y++  TA FI +   +RVALQFPDELLKDSV++V AL+  L +     V +++MA
Sbjct: 4    ELEWSYEIGRTAEFIESNKLRRVALQFPDELLKDSVRIVTALRASLSEQ----VGLFLMA 59

Query: 1500 DTTYGSCCVDEVAAAHVNADCVVHYGHACFSPTSKLPAWFVFRKSSFQYKNCIDALLKYS 1321
            DT YGSCCVDEV A+HV+ADCVVHYGH C SPT+KLP+ FVF K+     +C+  LL Y+
Sbjct: 60   DTAYGSCCVDEVGASHVDADCVVHYGHTCLSPTTKLPSLFVFGKAPITVSHCVTNLLHYA 119

Query: 1320 CSVDKPLLVLFSLEYIYAAALLKGEVEVHLKRDDMSNKIIFAELVSSEMDP-SSLAVNNT 1144
                KP++VLF L+Y ++   ++  V     RD    ++ FA+++ S M+P S   V+N 
Sbjct: 120  QPTGKPVMVLFGLDYAHSIQQIRESVMAAGGRD-YKREVQFADVMCSVMNPLSDPQVSND 178

Query: 1143 LSKDDTF-----------VYQLGALKWVLHKDKKMEDYMIVWIGSESVALTNILLTYSLC 997
            ++ D              VY++G L W L +  KMEDY++ WIGS + A  NI+LTYS C
Sbjct: 179  IAADGVTSSNSFGTVSGNVYRIGGLVWELPEGSKMEDYLLFWIGSNNSAFANIVLTYSGC 238

Query: 996  ELVRYDPENSILLKDISNQERILKRRYYLVQRAKDADIVGIVIGTLGIAGYQKVICTLKN 817
            E+VRYD     ++ D+S Q RILKRRYYL+++AKDA+IV I++GTLG+AGY  +I  +K 
Sbjct: 239  EIVRYDASECSMVTDLSQQRRILKRRYYLMEKAKDANIVAILVGTLGVAGYLNMIHQMKE 298

Query: 816  LIKRAGKKPYSFVMGRPNPAKLANFPECNIFIYVACPETAMLDSKEFLSPLITPYEAILA 637
            LI  AGKK Y+ VMGRPNPAKLANFPEC+IFIYV+C +TA+LDSKE+L+P+ITP+EA+LA
Sbjct: 299  LITAAGKKVYTLVMGRPNPAKLANFPECDIFIYVSCAQTALLDSKEYLAPVITPFEAMLA 358

Query: 636  FSRGSEWTNSYSLSFSDILGSSEKEINDVDTEPHFSFIKGAYVAN-DTLSDDVDEKEEKA 460
            FSRGS+WT  Y + F D+  SS  E  +   E  FSFI+G YV   D   +D +E +E A
Sbjct: 359  FSRGSQWTGEYVMEFRDLKNSSPVESRNQSEEARFSFIQGGYVEGFDREGNDAEEDKEGA 418

Query: 459  IALANEAKKALRLRDDSSNSGCLNITAKSGAEFLTLRSYQGLEISKETKQSYTSIKGRSG 280
            +ALAN  +KAL+LRDD + +     TAKSGAEFL  RSYQGL+I  +T      + GR G
Sbjct: 419  LALANVTEKALQLRDDPNTTA--KRTAKSGAEFLAARSYQGLDIHYDTSLPGPYLMGRKG 476

Query: 279  RAWSYQDE 256
            RA  YQDE
Sbjct: 477  RAAGYQDE 484


>ref|XP_002273627.2| PREDICTED: diphthamide biosynthesis protein 2-like [Vitis vinifera]
            gi|296084273|emb|CBI24661.3| unnamed protein product
            [Vitis vinifera]
          Length = 506

 Score =  471 bits (1212), Expect = e-130
 Identities = 242/501 (48%), Positives = 336/501 (67%), Gaps = 25/501 (4%)
 Frame = -1

Query: 1683 MELNALYDVEATATFIHTRGFKRVALQFPDELLKDSVKVVRALKTQLQQNSYDG------ 1522
            MEL + Y++  TA FI +R F RVALQFPDELLKD+ +VV+AL+ +L+  S  G      
Sbjct: 1    MELESNYEIALTADFIRSRNFTRVALQFPDELLKDATRVVKALRDRLESPSESGSGDGKE 60

Query: 1521 VQMYVMADTTYGSCCVDEVAAAHVNADCVVHYGHACFSPTSKLPAWFVFRKSSFQYKNCI 1342
            V ++VMADTTYGSCCVDEV A+H+NADCVVHYGH C SPTS LPA+FVF K+     NC 
Sbjct: 61   VGLFVMADTTYGSCCVDEVGASHINADCVVHYGHTCLSPTSNLPAFFVFGKAPISISNCA 120

Query: 1341 DALLKYSCSVDKPLLVLFSLEYIYAAALLKGEVEVHLKRDDMSNKII---FAELVSSEMD 1171
            + L  Y+ +  K ++VLF LEY +A  ++K  + +   +   S   +   FAE++ S M+
Sbjct: 121  ENLSTYALTQGKHIMVLFGLEYSHAIEMIKEALMLESSKLSGSTSNLEFHFAEVMCSVMN 180

Query: 1170 PS--------------SLAVNNTLSKDDTFVYQLGALKWVLHKDKKMEDYMIVWIGSESV 1033
            PS              S   N    K     Y +G L W L +  +MEDY++VWIG+++ 
Sbjct: 181  PSKDCTTSNGHLGSVGSFTANEGFGKATGTRYSIGGLAWNLPEGHRMEDYLLVWIGADNS 240

Query: 1032 ALTNILLTYSLCELVRYDPENSILLKDISNQERILKRRYYLVQRAKDADIVGIVIGTLGI 853
            A  N++LT++ C++VRYD  ++ L+ D+S Q RILKRRYYLV++AKD++I+G+++GTLG+
Sbjct: 241  AFANVVLTFNGCDIVRYDAVDNRLVTDLSQQRRILKRRYYLVEKAKDSNIIGLLVGTLGV 300

Query: 852  AGYQKVICTLKNLIKRAGKKPYSFVMGRPNPAKLANFPECNIFIYVACPETAMLDSKEFL 673
            AGY  +I  +K LI  AGKK Y+ VMGRPNPAKLANFPEC++FIYV+C +TA+LDSKEFL
Sbjct: 301  AGYLHMIRQMKELIAAAGKKAYTLVMGRPNPAKLANFPECDVFIYVSCAQTALLDSKEFL 360

Query: 672  SPLITPYEAILAFSRGSEWTNSYSLSFSDILGSSEKEINDVDTEPHFSFIKGAYVANDTL 493
            +P+ITP+EA+LAF+RGS+WT +Y + F D++ SS  E+ +   E  FSF++G YV +  L
Sbjct: 361  APVITPFEAMLAFNRGSQWTGAYVMEFRDLISSSPVEVRNQSEEARFSFLQGGYVEDFDL 420

Query: 492  --SDDVDEKEEKAIALANEAKKALRLRDDSSNSGCLNITAKSGAEFLTLRSYQGLEISKE 319
              ++D +E E+ A+ALA   +KAL++RD   NS  +  TAKSG EF   RSY GLE+   
Sbjct: 421  KETEDAEEDEDGALALAYATEKALQVRDKHPNS-LIKGTAKSGGEFFAARSYHGLEMHSN 479

Query: 318  TKQSYTSIKGRSGRAWSYQDE 256
                   + GR G+A  Y+ E
Sbjct: 480  DSSPEPYLIGRVGKASGYEGE 500


>ref|XP_002530564.1| diphteria toxin resistance protein 2, dph2, putative [Ricinus
            communis] gi|223529863|gb|EEF31794.1| diphteria toxin
            resistance protein 2, dph2, putative [Ricinus communis]
          Length = 499

 Score =  469 bits (1208), Expect = e-129
 Identities = 240/500 (48%), Positives = 343/500 (68%), Gaps = 21/500 (4%)
 Frame = -1

Query: 1683 MELNALYDVEATATFIHTRGFKRVALQFPDELLKDSVKVVRALKTQLQQNSYDG-----V 1519
            M+    Y+++ T  FIH+R F RVALQFPDELLKDSV+VV AL+ +L+    +G     +
Sbjct: 1    MDFEENYEIDLTTDFIHSRNFTRVALQFPDELLKDSVRVVSALRNKLELLEENGGDRKEI 60

Query: 1518 QMYVMADTTYGSCCVDEVAAAHVNADCVVHYGHACFSPTSKLPAWFVFRKSSFQYKNCID 1339
            +++VMADT YGSCCVDEV A+H NADCV+HYGH C SPTS LPA+FVF K+S    +C  
Sbjct: 61   RLFVMADTAYGSCCVDEVGASHANADCVIHYGHTCLSPTSNLPAFFVFGKASINVSSCAQ 120

Query: 1338 ALLKYSCSVDKPLLVLFSLEYIYAAALLKGEV--EVHLKRDDMSNKIIFAELVSSEMDPS 1165
            +L  Y+ S  KP++VL+ LEY +A   ++ E+     ++     +++ FA+++ S ++PS
Sbjct: 121  SLSDYAFSSTKPIMVLYGLEYAHAIPHIRQELIAAASMQSRPRHSELHFADVMCSAINPS 180

Query: 1164 S--------------LAVNNTLSKDDTFVYQLGALKWVLHKDKKMEDYMIVWIGSESVAL 1027
            +                  N + +     +++G L W L + ++MEDY++VWIGS + A 
Sbjct: 181  NNQKKSDEPARLIGDCVATNDVGEAAGCRHKIGGLIWDLSRGQRMEDYLLVWIGSGNSAF 240

Query: 1026 TNILLTYSLCELVRYDPENSILLKDISNQERILKRRYYLVQRAKDADIVGIVIGTLGIAG 847
             N++LT++ CE+VRYD + S L+ D+S Q RILKRRYYLV++AKDA+IVGI++GTLG+AG
Sbjct: 241  ANVVLTFNGCEIVRYDAKESRLVTDLSQQRRILKRRYYLVEKAKDANIVGILVGTLGVAG 300

Query: 846  YQKVICTLKNLIKRAGKKPYSFVMGRPNPAKLANFPECNIFIYVACPETAMLDSKEFLSP 667
            Y  +I  +K+LI  AGKK Y+ VMGRPNPAKLANFPEC+IFIYV+C +TA+LDSKEFL+P
Sbjct: 301  YLDMIHQMKDLITSAGKKAYTLVMGRPNPAKLANFPECDIFIYVSCAQTALLDSKEFLAP 360

Query: 666  LITPYEAILAFSRGSEWTNSYSLSFSDILGSSEKEINDVDTEPHFSFIKGAYVANDTLSD 487
            +ITP+EA++AF+RGS+WT +Y + F D++ SS  E      E  FSFI+G YV +  L +
Sbjct: 361  VITPFEAMIAFNRGSQWTGAYVMEFRDLIDSSPVEAMIKPAEARFSFIQGRYVEDFDLQE 420

Query: 486  DVDEKEEKAIALANEAKKALRLRDDSSNSGCLNITAKSGAEFLTLRSYQGLEISKETKQS 307
            +++E  E  +ALAN  +KAL+L D +S S  +  T KSGAE+   RSYQGLE+  +  + 
Sbjct: 421  NIEEDNE-GLALANATEKALQLHDRNSTS-LIKGTIKSGAEYFANRSYQGLEMHGDNSKP 478

Query: 306  YTSIKGRSGRAWSYQDEGHK 247
               + GR+G+A  Y++E  K
Sbjct: 479  GAFLIGRTGKASGYENEKTK 498


>gb|EXB55202.1| Diphthamide biosynthesis protein 2 [Morus notabilis]
          Length = 497

 Score =  469 bits (1207), Expect = e-129
 Identities = 248/493 (50%), Positives = 334/493 (67%), Gaps = 17/493 (3%)
 Frame = -1

Query: 1683 MELNALYDVEATATFIHTRGFKRVALQFPDELLKDSVKVVRALKTQLQQNSYDG----VQ 1516
            ME  + Y++  TA FI    F +VALQFPDELLKDSV+V  AL+ +L++   DG      
Sbjct: 1    MEFESRYEISRTAEFIRGGNFTKVALQFPDELLKDSVRVASALRNELRRLGGDGNVKDAS 60

Query: 1515 MYVMADTTYGSCCVDEVAAAHVNADCVVHYGHACFSPTSKLPAWFVFRKSSFQYKNCIDA 1336
            ++VMADT YGSCCVDEV A+HV ADCVVHYGHAC SPT+KLPA+FVF K+     +C   
Sbjct: 61   LFVMADTAYGSCCVDEVGASHVGADCVVHYGHACLSPTTKLPAFFVFGKAPMSVSDCTKN 120

Query: 1335 LLKYSCSVDKPLLVLFSLEYIYAAALLKGEVEVHLKRDDMSNKIIFAELVSSEMDPSSLA 1156
            L+  + +  KP+LVLF LEY +    ++ EV V         +I FAE++ S+M+PS+  
Sbjct: 121  LVDDAVTSGKPILVLFGLEYAHLIHNIREEV-VAACESRPQLEIQFAEVLCSDMNPSNKQ 179

Query: 1155 -------VNNTLSKDDTFVYQL------GALKWVLHKDKKMEDYMIVWIGSESVALTNIL 1015
                   V  ++  DD    +L      G L W L + +KMED+++ WIGS++ A  N++
Sbjct: 180  NTSDQGMVRASVLIDDKSFGKLAGNRGIGGLVWKLREGRKMEDHLLFWIGSDNSAFVNVV 239

Query: 1014 LTYSLCELVRYDPENSILLKDISNQERILKRRYYLVQRAKDADIVGIVIGTLGIAGYQKV 835
            LT++ CE+VRYD   + ++ D S Q+RILKRRYYLV++AKDA+IVGI++GTLG+AGY  +
Sbjct: 240  LTFNACEIVRYDAAENCMVTDFSQQKRILKRRYYLVEKAKDANIVGILVGTLGVAGYHHM 299

Query: 834  ICTLKNLIKRAGKKPYSFVMGRPNPAKLANFPECNIFIYVACPETAMLDSKEFLSPLITP 655
            I  +K LI  AGKK Y+ VMGRPNPAKLANFPEC+IFIYV+C +TA+LDSKE+LSP+ITP
Sbjct: 300  INQMKELITAAGKKAYTLVMGRPNPAKLANFPECDIFIYVSCAQTALLDSKEYLSPVITP 359

Query: 654  YEAILAFSRGSEWTNSYSLSFSDILGSSEKEINDVDTEPHFSFIKGAYVANDTLSDDVDE 475
            +EA LAF+R S WT +Y + F D++ SS  E      E  FSF++G YV +    ++  E
Sbjct: 360  FEATLAFNRDSHWTGAYVMEFRDLINSSLAESRSHSKEARFSFLQGGYVEDFDTQENDKE 419

Query: 474  KEEKAIALANEAKKALRLRDDSSNSGCLNITAKSGAEFLTLRSYQGLEISKETKQSYTSI 295
            +EE A+ALAN  +KAL+LRD++++   +  T KSGAE+L  RSYQGLEI  E       +
Sbjct: 420  EEEGALALANATEKALQLRDNANS--LVKRTVKSGAEYLMTRSYQGLEIHNENSLPEPYL 477

Query: 294  KGRSGRAWSYQDE 256
             GRSGRA  Y+DE
Sbjct: 478  IGRSGRAAGYKDE 490


>ref|XP_002326199.1| predicted protein [Populus trichocarpa]
            gi|566175949|ref|XP_006381405.1| diphthamide synthesis
            DPH2 family protein [Populus trichocarpa]
            gi|550336108|gb|ERP59202.1| diphthamide synthesis DPH2
            family protein [Populus trichocarpa]
          Length = 496

 Score =  465 bits (1196), Expect = e-128
 Identities = 237/494 (47%), Positives = 328/494 (66%), Gaps = 18/494 (3%)
 Frame = -1

Query: 1683 MELNALYDVEATATFIHTRGFKRVALQFPDELLKDSVKVVRALKTQLQQ-NSYDGVQMYV 1507
            M+L  +Y+++ T  FIH+R F RVALQFPDELLKD  KVVRAL+ ++ +     GV ++V
Sbjct: 1    MDLMEIYEIDRTVDFIHSRNFTRVALQFPDELLKDCTKVVRALREKIAKLKEQIGVSLFV 60

Query: 1506 MADTTYGSCCVDEVAAAHVNADCVVHYGHACFSPTSKLPAWFVFRKSSFQYKNCIDALLK 1327
            MAD+TYGSCCVDEV A+ +NADCV+HYGH C SPTS LPA+FVF K+     +C  +L  
Sbjct: 61   MADSTYGSCCVDEVGASRINADCVIHYGHTCLSPTSTLPAFFVFGKAMIDVSSCAASLSN 120

Query: 1326 YSCSVDKPLLVLFSLEYIYAAALLKGEVEVH---LKRDDMSNKIIFAELVSSEMDPSSL- 1159
            Y+ +  KP LVLF LEY +A   ++ E+      L          FAE++   ++P    
Sbjct: 121  YASNNSKPFLVLFGLEYAHAIPYIREELVAAASTLSGSKTKTVFHFAEVMCPLINPPDCH 180

Query: 1158 -------------AVNNTLSKDDTFVYQLGALKWVLHKDKKMEDYMIVWIGSESVALTNI 1018
                          V+N L       + +G L W L   +KMEDY+++WIGS++ A  N+
Sbjct: 181  KDSGGLPRSVGDGEVSNELGVAACSRHTIGGLSWELPSGQKMEDYLLLWIGSDNSAFANV 240

Query: 1017 LLTYSLCELVRYDPENSILLKDISNQERILKRRYYLVQRAKDADIVGIVIGTLGIAGYQK 838
            +LT++ CE+VRYD   + L  D+S Q RILKRRYYLV++AKDA+IVGI++GTLG+AGY  
Sbjct: 241  VLTFNGCEIVRYDANGNFLGTDLSQQRRILKRRYYLVEKAKDANIVGILVGTLGVAGYLN 300

Query: 837  VICTLKNLIKRAGKKPYSFVMGRPNPAKLANFPECNIFIYVACPETAMLDSKEFLSPLIT 658
            +I  +K+LI  AGKK Y+ VMGRPNPAKLANFPEC++FIYV+C +TA+LDSK+F +P+IT
Sbjct: 301  MIHQMKDLITGAGKKVYTLVMGRPNPAKLANFPECDVFIYVSCAQTALLDSKDFYAPVIT 360

Query: 657  PYEAILAFSRGSEWTNSYSLSFSDILGSSEKEINDVDTEPHFSFIKGAYVANDTLSDDVD 478
            P+EA+LAF+RGSEWT +Y + F D++ SS  E+     E  FSF++G YV +  L +  +
Sbjct: 361  PFEAMLAFNRGSEWTGAYVMEFRDLITSSPVEVKSYSEEARFSFVQGRYVEDFDLQEKKE 420

Query: 477  EKEEKAIALANEAKKALRLRDDSSNSGCLNITAKSGAEFLTLRSYQGLEISKETKQSYTS 298
            E+ E  +ALAN  +KAL+L+D SSNS     T +SGA++   RSY GL++  +       
Sbjct: 421  EENEGVLALANATEKALKLQDRSSNS-LTKGTVRSGADYFATRSYHGLDMHGDDSMLEPY 479

Query: 297  IKGRSGRAWSYQDE 256
            + GR+G+A  YQ E
Sbjct: 480  LIGRTGKASGYQHE 493


>ref|XP_004152127.1| PREDICTED: diphthamide biosynthesis protein 2-like [Cucumis sativus]
            gi|449484704|ref|XP_004156957.1| PREDICTED: diphthamide
            biosynthesis protein 2-like [Cucumis sativus]
          Length = 500

 Score =  464 bits (1193), Expect = e-128
 Identities = 244/503 (48%), Positives = 333/503 (66%), Gaps = 24/503 (4%)
 Frame = -1

Query: 1683 MELNALYDVEATATFIHTRGFKRVALQFPDELLKDSVKVVRALKTQLQ---------QNS 1531
            ME  + Y++  TA FIHTR F RVALQFPDELLKDS +VVRALK +L+          +S
Sbjct: 1    MEFESYYEISRTADFIHTRNFTRVALQFPDELLKDSTRVVRALKDRLRVLDESDTNHSDS 60

Query: 1530 YDGVQMYVMADTTYGSCCVDEVAAAHVNADCVVHYGHACFSPTSKLPAWFVFRKSSFQYK 1351
               V+++VMADTTYGSCCVDEV AAH NADCV+HYGH C SPT+ LPA FVF K S    
Sbjct: 61   KSEVRLFVMADTTYGSCCVDEVGAAHANADCVIHYGHTCLSPTTTLPALFVFGKDSIDVP 120

Query: 1350 NCIDALLKYSCSVDKPLLVLFSLEYIYAAALLKGEVEVHLKRDDMSNK--IIFAELVSSE 1177
             C   L +YS S  KP+LVLF LEY ++   +K  +    +   + ++  + FA++  S 
Sbjct: 121  TCAKTLSQYSLSSGKPVLVLFGLEYAHSMLDIKQRLLDSFQTSSLGSELEVHFADVKCSS 180

Query: 1176 MDPS----------SLAVNNTLSKDDTFV---YQLGALKWVLHKDKKMEDYMIVWIGSES 1036
            +DPS            A N      ++     + +G L W L K+++++D  + WIGSE+
Sbjct: 181  LDPSPHHENVVKGGEQASNAESGSSESIAGARHHIGGLFWELPKERRVKDCSLFWIGSEN 240

Query: 1035 VALTNILLTYSLCELVRYDPENSILLKDISNQERILKRRYYLVQRAKDADIVGIVIGTLG 856
             A  N++LT++ CE+VRYD + S L+ D+S Q RILKRRYYLV++AKDA IVGI++GTLG
Sbjct: 241  SAFANVVLTFNGCEIVRYDAKESWLVTDVSKQRRILKRRYYLVEKAKDAAIVGILVGTLG 300

Query: 855  IAGYQKVICTLKNLIKRAGKKPYSFVMGRPNPAKLANFPECNIFIYVACPETAMLDSKEF 676
            +AGY  +I  +K LI  AGKK Y+ VMG+PNPAKLANFPEC +FIYV+C +TA++DSKE+
Sbjct: 301  LAGYLHIIHQMKELITGAGKKAYTLVMGKPNPAKLANFPECGVFIYVSCAQTALMDSKEY 360

Query: 675  LSPLITPYEAILAFSRGSEWTNSYSLSFSDILGSSEKEINDVDTEPHFSFIKGAYVANDT 496
            L+P+ITP+EA LAFSRGS+WT +Y + F D++  S  E  +   E  +SF++G YV  D 
Sbjct: 361  LAPVITPFEATLAFSRGSQWTGAYVMEFQDLIDFSTPEEANRSDEARYSFLQGGYV-EDW 419

Query: 495  LSDDVDEKEEKAIALANEAKKALRLRDDSSNSGCLNITAKSGAEFLTLRSYQGLEISKET 316
             S +  E+E  A AL    +KAL+LRD  + +  +  TA+SGAEF   RS+QGL+I+  +
Sbjct: 420  DSQENTEEENGAHALVTATEKALQLRD--NRNSLIEGTARSGAEFFATRSFQGLDINNGS 477

Query: 315  KQSYTSIKGRSGRAWSYQDEGHK 247
             +    + GRSG+A  YQDE ++
Sbjct: 478  LEPEPYVIGRSGKASGYQDEKNR 500


>gb|ESW22742.1| hypothetical protein PHAVU_005G177500g [Phaseolus vulgaris]
          Length = 501

 Score =  463 bits (1192), Expect = e-128
 Identities = 237/505 (46%), Positives = 337/505 (66%), Gaps = 26/505 (5%)
 Frame = -1

Query: 1683 MELNALYDVEATATFIHTRGFKRVALQFPDELLKDSVKVVRALKTQLQQ---------NS 1531
            M+  + YD+ A+A FIHT  F RVALQFPD LLKDS +VV AL+ +LQ            
Sbjct: 1    MDFESNYDIAASAQFIHTHNFTRVALQFPDNLLKDSTRVVTALRKRLQSLKEIDATESGH 60

Query: 1530 YDGVQMYVMADTTYGSCCVDEVAAAHVNADCVVHYGHACFSPTSKLPAWFVFRKSSFQYK 1351
               V ++VMADT YGSCCVDEV A+H+NADCV+HYGH CFSPT+ LP++FVF K+S    
Sbjct: 61   ETDVGLFVMADTAYGSCCVDEVGASHINADCVIHYGHTCFSPTTTLPSFFVFGKASICIA 120

Query: 1350 NCIDALLKYSCSVDKPLLVLFSLEYIYAA-----ALLKGEVEVHLKRDDMSNKIIFAELV 1186
            +C++++ KY+ +  KP++V+F LEY ++      ALL+  V     R D    + FA+  
Sbjct: 121  DCVESMSKYALTNSKPIMVVFGLEYAHSMQQIKEALLESSVSC---RFDPKPDVHFADFP 177

Query: 1185 SSEMDPSSLAVN--------NTLSKDDTFVYQLGALKWVLHKDKKMEDYMIVWIGSESVA 1030
            SS M PS             +  + ++   Y +G L W L + + M+DY++ WIG +  A
Sbjct: 178  SSVMFPSKDIKKIKGLQEPASGCNDENGTTYSIGGLTWKLPEGQSMDDYLLFWIGLDDSA 237

Query: 1029 LTNILLTYSLCELVRYDPENSILLKDISNQERILKRRYYLVQRAKDADIVGIVIGTLGIA 850
              N++LT++ CE+VRYD   + ++ D+  Q RILKRRYYLV+RAKDA+IVGI++GTLG+A
Sbjct: 238  FANVVLTFNTCEIVRYDANENQVVTDLFQQRRILKRRYYLVERAKDANIVGILVGTLGVA 297

Query: 849  GYQKVICTLKNLIKRAGKKPYSFVMGRPNPAKLANFPECNIFIYVACPETAMLDSKEFLS 670
            GY  +I  +K LI  AGKK Y+ VMGRPNPAKLANFPEC++F+YV+C +TA+LDSKE+L+
Sbjct: 298  GYLHIINQMKELITAAGKKAYTLVMGRPNPAKLANFPECDVFLYVSCAQTALLDSKEYLA 357

Query: 669  PLITPYEAILAFSRGSEWTNSYSLSFSDILGSSEKEINDVDTEPHFSFIKGAYVAN---- 502
            P+ITP+EA++AF+RGS+WT +Y + F D++   + E+ D + E  FSF+KG Y+ +    
Sbjct: 358  PVITPFEAMIAFNRGSQWTGAYVMEFRDLINLPQMEVGDQEEEARFSFLKGGYIEDFENQ 417

Query: 501  DTLSDDVDEKEEKAIALANEAKKALRLRDDSSNSGCLNITAKSGAEFLTLRSYQGLEISK 322
            + LS++V E E + +AL N  +KAL+LR++S  +  +   A+SGAEFL  RSYQGL +  
Sbjct: 418  ENLSENVGE-EREVLALVNATEKALQLRNNS--NALMKGNARSGAEFLANRSYQGLNMPS 474

Query: 321  ETKQSYTSIKGRSGRAWSYQDEGHK 247
            +       + GR GRA  Y+DE +K
Sbjct: 475  DNASPEPYLIGRRGRASGYEDEKNK 499


>ref|XP_004498540.1| PREDICTED: diphthamide biosynthesis protein 2-like [Cicer arietinum]
          Length = 501

 Score =  458 bits (1179), Expect = e-126
 Identities = 240/507 (47%), Positives = 337/507 (66%), Gaps = 28/507 (5%)
 Frame = -1

Query: 1683 MELNALYDVEATATFIHTRGFKRVALQFPDELLKDSVKVVRALKTQLQQ---------NS 1531
            M+  + YD+ ATA FI T  F RVALQFPD+LLKDS KVV  L+ +LQ            
Sbjct: 1    MDFESNYDIAATAHFILTHNFTRVALQFPDDLLKDSTKVVTVLRKRLQSLRECDITGSGD 60

Query: 1530 YDGVQMYVMADTTYGSCCVDEVAAAHVNADCVVHYGHACFSPTSKLPAWFVFRKSSFQYK 1351
               + +YVMADT YGSCCVDEV A+H+NADCV+HYGH CFSPT+ LPA+FVF KSS    
Sbjct: 61   ETEIGLYVMADTAYGSCCVDEVGASHINADCVIHYGHTCFSPTTTLPAFFVFGKSSICAA 120

Query: 1350 NCIDALLKYSCSVDKPLLVLFSLEYIYAAALLKGEVEVHLK-----RDDMSNKIIFAELV 1186
            +C++ L +Y+ +  KP++VLF LEY ++   ++   +V L+     R D  ++  FA++ 
Sbjct: 121  DCVERLSQYALTNSKPIMVLFGLEYAHSIQQIR---KVLLESSTSCRSDFKSEFHFADVP 177

Query: 1185 SSEMDPS------------SLAVNNTLSKDDTF--VYQLGALKWVLHKDKKMEDYMIVWI 1048
            S+ M PS            +    +  S D     VY +G L W L + + MEDY + WI
Sbjct: 178  SAFMFPSKDIKKLNGLQEAACDCGHKSSSDGASGTVYNIGGLTWKLPEGQSMEDYSLFWI 237

Query: 1047 GSESVALTNILLTYSLCELVRYDPENSILLKDISNQERILKRRYYLVQRAKDADIVGIVI 868
            G ++ A  NI+LT++ CE+VRYD   + ++ D+S Q RILKRRYYLV+RAKDA+IVGI++
Sbjct: 238  GHDNSAFANIVLTFNACEIVRYDATKNQMVTDLSQQRRILKRRYYLVERAKDANIVGILV 297

Query: 867  GTLGIAGYQKVICTLKNLIKRAGKKPYSFVMGRPNPAKLANFPECNIFIYVACPETAMLD 688
            GTLG+AGY  +I  +  LI  AGKK Y+ VMG+PNPAKLANFPEC++F+YV+C +TA+LD
Sbjct: 298  GTLGVAGYLHIINQMMELITGAGKKAYTLVMGKPNPAKLANFPECDVFVYVSCAQTALLD 357

Query: 687  SKEFLSPLITPYEAILAFSRGSEWTNSYSLSFSDILGSSEKEINDVDTEPHFSFIKGAYV 508
            SKE+L+P+ITP+EA +AF+RGS+WT +Y + F D++   + ++ + + E  FSF++G YV
Sbjct: 358  SKEYLAPVITPFEATIAFNRGSQWTGAYVMGFGDLINLPQVQVGNQE-EARFSFLQGGYV 416

Query: 507  ANDTLSDDVDEKEEKAIALANEAKKALRLRDDSSNSGCLNITAKSGAEFLTLRSYQGLEI 328
             +    ++V+E E + +ALAN  +KAL+LRD+ ++   + + AKSGAEF   RSYQGL +
Sbjct: 417  KDFENQENVEE-ERETLALANATEKALQLRDNCNS--LIKVDAKSGAEFFANRSYQGLNM 473

Query: 327  SKETKQSYTSIKGRSGRAWSYQDEGHK 247
              E         GRSGRA  Y+DE +K
Sbjct: 474  PDENTSPEPFFIGRSGRAAGYEDEKNK 500


>ref|XP_006478781.1| PREDICTED: diphthamide biosynthesis protein 2-like isoform X1 [Citrus
            sinensis]
          Length = 495

 Score =  456 bits (1173), Expect = e-125
 Identities = 237/492 (48%), Positives = 337/492 (68%), Gaps = 13/492 (2%)
 Frame = -1

Query: 1683 MELNALYDVEATATFIHTRGFKRVALQFPDELLKDSVKVVRALKTQLQQ-NSYDG----- 1522
            M+    Y++ +TA FI++R F RVALQFPD LLKDS+++VRAL+ +L    S DG     
Sbjct: 1    MDFELNYEIASTADFIYSRKFTRVALQFPDNLLKDSIRIVRALRAKLGSLRSSDGERGVD 60

Query: 1521 ---VQMYVMADTTYGSCCVDEVAAAHVNADCVVHYGHACFSPTSKLPAWFVFRKSSFQYK 1351
               + ++VMADTTYGSCCVDEV A+H++ADCV+HYGH C SPTS LPA+FVF K+S    
Sbjct: 61   DKDIGLFVMADTTYGSCCVDEVGASHIDADCVIHYGHTCLSPTSTLPAFFVFGKASINTS 120

Query: 1350 NCIDALLKYSCSVDKPLLVLFSLEYIYAAALLKGEVEVHLKR--DDMSNKIIFAELVSSE 1177
            NCI+ L K++ +  KP+LVL+ LEY +A   ++  V+V        +  +I FA+++ + 
Sbjct: 121  NCIENLSKHALTNGKPILVLYGLEYAHAIPHIREAVKVASSSYGSGLKLEINFADVMCTV 180

Query: 1176 MDPSSLAVNNTLSKDDTFVYQLGALKWVLHKDKKMEDYMIVWIGSESVALTNILLTYSLC 997
            + PS    +          + +G L W +   KKME++++ WIGS++ A  N++LT++ C
Sbjct: 181  ITPSKDHKSLCGPAGGCTRHTIGGLVWNIPDRKKMEEHLLFWIGSDNSAFANVVLTFNGC 240

Query: 996  ELVRYDPENSILLKDISNQERILKRRYYLVQRAKDADIVGIVIGTLGIAGYQKVICTLKN 817
            E+VRYD     LL D+S   +ILKRRYYLV++AKDA+I+G+++GTLG+AGY  +I  +K 
Sbjct: 241  EIVRYDATEERLLTDVSQPLKILKRRYYLVEKAKDANIIGVLVGTLGVAGYLHMIHQMKE 300

Query: 816  LIKRAGKKPYSFVMGRPNPAKLANFPECNIFIYVACPETAMLDSKEFLSPLITPYEAILA 637
            LI +AGKK Y+ VMG+PNPAKLANFPEC++FI V+C +TA+LDSKEFL+P+ITP+EA+LA
Sbjct: 301  LITKAGKKAYTLVMGKPNPAKLANFPECDVFINVSCAQTALLDSKEFLAPVITPFEAMLA 360

Query: 636  FSRGSEWTNSYSLSFSDILGSSEKEINDVDTEPHFSFIKGAYVAN--DTLSDDVDEKEEK 463
            F RG++WT +Y + F D++ SS  E +D   E  FSF+KG YV +     +++ +E++E 
Sbjct: 361  FGRGTQWTGAYVMEFRDLMSSSPVEGSDQAEEARFSFLKGGYVEDVAQPETENGEEEKEG 420

Query: 462  AIALANEAKKALRLRDDSSNSGCLNITAKSGAEFLTLRSYQGLEISKETKQSYTSIKGRS 283
            A+ALAN A+KAL+LRD S +   +  TAKSGAE+   RSY GLE+   +        GRS
Sbjct: 421  ALALANAAEKALQLRDRSLDP-IVKGTAKSGAEYFVSRSYHGLEMQCNSSSPEPYAIGRS 479

Query: 282  GRAWSYQDEGHK 247
            G+A  Y DE  K
Sbjct: 480  GKASGYDDEKSK 491


>ref|XP_004249376.1| PREDICTED: diphthamide biosynthesis protein 2-like [Solanum
            lycopersicum]
          Length = 498

 Score =  456 bits (1173), Expect = e-125
 Identities = 243/497 (48%), Positives = 326/497 (65%), Gaps = 21/497 (4%)
 Frame = -1

Query: 1683 MELNALYDVEATATFIHTRGFKRVALQFPDELLKDSVKVVRALKTQLQQNSY-------- 1528
            M+L + Y+++  A FI  RGF+RVALQFPD LLKDS ++V AL  +L+  S         
Sbjct: 1    MDLESTYEIDRVAGFISARGFRRVALQFPDNLLKDSTRIVAALHEKLRSFSQLHAGRNGD 60

Query: 1527 -DGVQMYVMADTTYGSCCVDEVAAAHVNADCVVHYGHACFSPTSKLPAWFVFRKSSFQYK 1351
               V++YVMADT YG+CCVDEV A+H NADCV+HYGH CFSPTS LPA+ V  K+S    
Sbjct: 61   AKDVKLYVMADTMYGNCCVDEVGASHANADCVIHYGHTCFSPTSTLPAFLVLGKASLNVP 120

Query: 1350 NCIDALLKYSCSVDKPLLVLFSLEYIYAAALLKG--------EVEVHLKRDDMSNKIIFA 1195
             C   L KY+    KP+LVL+ LEY +    +K         E+EVH   D +S  I   
Sbjct: 121  LCAQTLCKYTIKAGKPILVLYGLEYTHTITEIKASVGAQVCSELEVHYA-DIISPIITPP 179

Query: 1194 ELVSSEMDPSSLA----VNNTLSKDDTFVYQLGALKWVLHKDKKMEDYMIVWIGSESVAL 1027
            E  SS  +   L+     N    + +  +Y +G L W L    ++EDY+I ++GS+  A 
Sbjct: 180  ETFSSMNEQPELSDGQCANGCSIEVNDAIYCVGGLTWSLPVGHRIEDYLIFYVGSDDPAF 239

Query: 1026 TNILLTYSLCELVRYDPENSILLKDISNQERILKRRYYLVQRAKDADIVGIVIGTLGIAG 847
             NIL+TY+ CE+VRYD     L+ + S Q+RILKRRY+L+++AKDA I+GI++GTLG+AG
Sbjct: 240  ANILMTYNACEIVRYDATEDKLVNEFSQQKRILKRRYFLIEKAKDASIIGILVGTLGVAG 299

Query: 846  YQKVICTLKNLIKRAGKKPYSFVMGRPNPAKLANFPECNIFIYVACPETAMLDSKEFLSP 667
            Y  +I  +K+LI RAGKK Y+FVMGRPNPAKLANFPEC+IF+YV+C +TA+LDSKEFL+P
Sbjct: 300  YLHMIHQMKDLITRAGKKAYTFVMGRPNPAKLANFPECDIFVYVSCAQTALLDSKEFLAP 359

Query: 666  LITPYEAILAFSRGSEWTNSYSLSFSDILGSSEKEINDVDTEPHFSFIKGAYVANDTLSD 487
            +ITP+EA++AF RGSEWT +Y   F D++ SS  E  +  +E  FSFI+G YV  D    
Sbjct: 360  VITPFEAMIAFGRGSEWTGAYVTEFRDLITSSPMEAKN-QSEARFSFIQGGYV-EDVEQQ 417

Query: 486  DVDEKEEKAIALANEAKKALRLRDDSSNSGCLNITAKSGAEFLTLRSYQGLEISKETKQS 307
            +V+E E+   AL N  +KALR+RD  S +  +  TAKSGAE+   RSY GL+I  E   S
Sbjct: 418  EVEEVEDGVSALVNITEKALRVRDKDSQT-LMPGTAKSGAEYFATRSYHGLDIHPENNFS 476

Query: 306  YTSIKGRSGRAWSYQDE 256
               + G+SGRA  Y++E
Sbjct: 477  EPFLIGKSGRASGYRNE 493


>ref|XP_003546007.1| PREDICTED: diphthamide biosynthesis protein 2-like isoform X1
            [Glycine max]
          Length = 485

 Score =  456 bits (1173), Expect = e-125
 Identities = 234/493 (47%), Positives = 336/493 (68%), Gaps = 14/493 (2%)
 Frame = -1

Query: 1683 MELNALYDVEATATFIHTRGFKRVALQFPDELLKDSVKVVRALKTQLQQNSYDGVQMYVM 1504
            M+  + YD+ A+A FIHT  F +VALQFPD LLK S +VV AL+  LQ      V ++VM
Sbjct: 1    MDFESNYDIAASANFIHTHNFIKVALQFPDNLLKHSTRVVTALRNTLQSTE---VGLFVM 57

Query: 1503 ADTTYGSCCVDEVAAAHVNADCVVHYGHACFSPTSKLPAWFVFRKSSFQYKNCIDALLKY 1324
            ADT YGSCC+DEV A+H+NADCV+HYGH CFSPT+ LPA+ VF K+S    +C++++ KY
Sbjct: 58   ADTAYGSCCIDEVGASHINADCVIHYGHTCFSPTTTLPAFCVFGKASICIADCVESMSKY 117

Query: 1323 SCSVDKPLLVLFSLEYIYAA-----ALLKGEVEVHLKRDDMSNKIIFAELVSSEMDPSS- 1162
            + +  KP++VLF LEY ++      ALL+  +     R D  +++ FA++ SS M PS  
Sbjct: 118  ALTNSKPIMVLFGLEYAHSMQRIKEALLESSMSC---RFDPKSEVHFADVPSSVMFPSKD 174

Query: 1161 LAVNNTLSKDDT-------FVYQLGALKWVLHKDKKMEDYMIVWIGSESVALTNILLTYS 1003
            +   N L +  +        +Y +G L W L + + M+DY++ WIG +  A  N++LT++
Sbjct: 175  IKKINVLQEPVSDCNGASGTMYSIGGLTWKLPEGQSMDDYLVFWIGHDDSAFANVVLTFN 234

Query: 1002 LCELVRYDPENSILLKDISNQERILKRRYYLVQRAKDADIVGIVIGTLGIAGYQKVICTL 823
             CE+VRYD   + ++ D+    RILKRRYYLV+RAKDA+IVGI++GTLG+AGY  +I  +
Sbjct: 235  ACEIVRYDANENRMVTDLFQHRRILKRRYYLVERAKDANIVGILVGTLGVAGYLHIINQM 294

Query: 822  KNLIKRAGKKPYSFVMGRPNPAKLANFPECNIFIYVACPETAMLDSKEFLSPLITPYEAI 643
              LI  AGKK Y+ VMGRPNPAKLANFPEC++F+YV+C +TA+LDSKE+L+P+ITP+EA+
Sbjct: 295  MELITGAGKKAYTLVMGRPNPAKLANFPECDVFLYVSCAQTALLDSKEYLAPVITPFEAM 354

Query: 642  LAFSRGSEWTNSYSLSFSDILGSSEKEI-NDVDTEPHFSFIKGAYVANDTLSDDVDEKEE 466
            +AF+RGS+WT +Y + F D++   +  + N  + E  FSF+KG YV +    ++V+E E 
Sbjct: 355  IAFNRGSQWTGAYVMEFRDLINLPQMGVGNQEEEEARFSFLKGGYVEDFENQENVEE-ER 413

Query: 465  KAIALANEAKKALRLRDDSSNSGCLNITAKSGAEFLTLRSYQGLEISKETKQSYTSIKGR 286
            + +AL N  +KAL+LR++S  +  +  +A+SGAEFL  RSYQGL +S E       + GR
Sbjct: 414  ETLALVNATEKALQLRNNS--NVLMKGSARSGAEFLANRSYQGLNMSSENTSPEPYLIGR 471

Query: 285  SGRAWSYQDEGHK 247
             GRA  Y+DE +K
Sbjct: 472  RGRASGYEDEKNK 484


>ref|XP_006597121.1| PREDICTED: diphthamide biosynthesis protein 2-like isoform X2
            [Glycine max]
          Length = 490

 Score =  454 bits (1168), Expect = e-125
 Identities = 234/497 (47%), Positives = 336/497 (67%), Gaps = 18/497 (3%)
 Frame = -1

Query: 1683 MELNALYDVEATATFIHTRGFKRVALQFPDELLKDSVKVVRALKTQLQQNSYDGVQMYVM 1504
            M+  + YD+ A+A FIHT  F +VALQFPD LLK S +VV AL+  LQ      V ++VM
Sbjct: 1    MDFESNYDIAASANFIHTHNFIKVALQFPDNLLKHSTRVVTALRNTLQSTE---VGLFVM 57

Query: 1503 ADTTYGSCCVDEVAAAHVNADCVVHYGHACFSPTSKLPAWFVFRKSSFQYKNCIDALLKY 1324
            ADT YGSCC+DEV A+H+NADCV+HYGH CFSPT+ LPA+ VF K+S    +C++++ KY
Sbjct: 58   ADTAYGSCCIDEVGASHINADCVIHYGHTCFSPTTTLPAFCVFGKASICIADCVESMSKY 117

Query: 1323 SCSVDKPLLVLFSLEYIYAA-----ALLKGEVEVHLKRDDMSNKIIFAELVSSEMDPSS- 1162
            + +  KP++VLF LEY ++      ALL+  +     R D  +++ FA++ SS M PS  
Sbjct: 118  ALTNSKPIMVLFGLEYAHSMQRIKEALLESSMSC---RFDPKSEVHFADVPSSVMFPSKD 174

Query: 1161 LAVNNTLSKDDT-------FVYQLGALKWVLHKDKKMEDYMIVWIGSESVALTNILLTYS 1003
            +   N L +  +        +Y +G L W L + + M+DY++ WIG +  A  N++LT++
Sbjct: 175  IKKINVLQEPVSDCNGASGTMYSIGGLTWKLPEGQSMDDYLVFWIGHDDSAFANVVLTFN 234

Query: 1002 LCELVRYDPENSILLKDISNQERILKRRYYLVQRAKDADIVGIVIGTLGIAGYQKVICTL 823
             CE+VRYD   + ++ D+    RILKRRYYLV+RAKDA+IVGI++GTLG+AGY  +I  +
Sbjct: 235  ACEIVRYDANENRMVTDLFQHRRILKRRYYLVERAKDANIVGILVGTLGVAGYLHIINQM 294

Query: 822  KNLIKRAGKKPYSFVMGRPNPAKLANFPECNIFIYVACPETAMLDSKEFLSPLITPYEAI 643
              LI  AGKK Y+ VMGRPNPAKLANFPEC++F+YV+C +TA+LDSKE+L+P+ITP+EA+
Sbjct: 295  MELITGAGKKAYTLVMGRPNPAKLANFPECDVFLYVSCAQTALLDSKEYLAPVITPFEAM 354

Query: 642  LAFSRGSEWTNSYSLSFSDILGSSEKEI-NDVDTEPHFSFIKGAYVAN----DTLSDDVD 478
            +AF+RGS+WT +Y + F D++   +  + N  + E  FSF+KG YV +      +S +  
Sbjct: 355  IAFNRGSQWTGAYVMEFRDLINLPQMGVGNQEEEEARFSFLKGGYVEDFENQACISSENV 414

Query: 477  EKEEKAIALANEAKKALRLRDDSSNSGCLNITAKSGAEFLTLRSYQGLEISKETKQSYTS 298
            E+E + +AL N  +KAL+LR++S  +  +  +A+SGAEFL  RSYQGL +S E       
Sbjct: 415  EEERETLALVNATEKALQLRNNS--NVLMKGSARSGAEFLANRSYQGLNMSSENTSPEPY 472

Query: 297  IKGRSGRAWSYQDEGHK 247
            + GR GRA  Y+DE +K
Sbjct: 473  LIGRRGRASGYEDEKNK 489


>gb|EOY21113.1| Diphthamide synthesis DPH2 family protein [Theobroma cacao]
          Length = 833

 Score =  454 bits (1167), Expect = e-125
 Identities = 233/489 (47%), Positives = 331/489 (67%), Gaps = 13/489 (2%)
 Frame = -1

Query: 1683 MELNALYDVEATATFIHTRGFKRVALQFPDELLKDSVKVVRALKTQLQQNSYDGVQMYVM 1504
            M+L   +++  TA FI +R F RVALQFPD+LLK S KVV +L+ +L   S + V ++VM
Sbjct: 344  MDLETNFEISRTAEFILSRNFTRVALQFPDDLLKHSTKVVTSLRNKL--GSGNKVGLFVM 401

Query: 1503 ADTTYGSCCVDEVAAAHVNADCVVHYGHACFSPTSKLPAWFVFRKSSFQYKNCIDALLKY 1324
            ADT YGSCCVDEV A+H++ADCV+HYGH C SPTS LP++ VF K+S    +C++ L  +
Sbjct: 402  ADTAYGSCCVDEVGASHIDADCVIHYGHTCLSPTSTLPSFCVFGKASISVFSCVEKLSSF 461

Query: 1323 SCSVDKPLLVLFSLEYIYAAALLKGEVEVHLKRDDMSNKIIFAELVSSEMDPSS------ 1162
              +  +P +VL+ LEY +A + +K  +     +  +  ++ FA+++ S ++P        
Sbjct: 462  FWTNCRPTVVLYGLEYAHAISQIKEALVEATPKAKV--ELQFADVMCSIINPLEDHRRSN 519

Query: 1161 -------LAVNNTLSKDDTFVYQLGALKWVLHKDKKMEDYMIVWIGSESVALTNILLTYS 1003
                      +N+   +    Y LG L W L + +KMEDYM++WIG ++ A  N++LT++
Sbjct: 520  GLLGHLGSCTSNSSGAETGSRYSLGGLIWDLPEGQKMEDYMLLWIGPDNSAFANVVLTFN 579

Query: 1002 LCELVRYDPENSILLKDISNQERILKRRYYLVQRAKDADIVGIVIGTLGIAGYQKVICTL 823
             CE+VRYD     L+ D+S Q+RILKRRYYLV+RAKDA++VGI++GTLG+AGY  +I  +
Sbjct: 580  GCEIVRYDAAEDQLVTDVSQQKRILKRRYYLVERAKDANMVGILVGTLGVAGYLHMIHQM 639

Query: 822  KNLIKRAGKKPYSFVMGRPNPAKLANFPECNIFIYVACPETAMLDSKEFLSPLITPYEAI 643
            K LI  AGKK Y+ VMGRPNPAKLANFPEC++FIYV+C +TA+LDSKEFL+P+ITP+EA+
Sbjct: 640  KELIMGAGKKAYTLVMGRPNPAKLANFPECDVFIYVSCAQTALLDSKEFLAPVITPFEAL 699

Query: 642  LAFSRGSEWTNSYSLSFSDILGSSEKEINDVDTEPHFSFIKGAYVANDTLSDDVDEKEEK 463
            LAF+RGS+WT +Y + F D++ SS  E+     E  FSF+KG YV +  L ++ DE+ E 
Sbjct: 700  LAFNRGSQWTGTYVMEFRDLINSSPLEVKKQPEEARFSFLKGGYVEDFELHENGDEENEG 759

Query: 462  AIALANEAKKALRLRDDSSNSGCLNITAKSGAEFLTLRSYQGLEISKETKQSYTSIKGRS 283
            ++ALAN  +K L+LRD +  S  +   AKSGAEF   RSY GLE+   +      + GRS
Sbjct: 760  SLALANATEKVLQLRDTNPGS-LVKRVAKSGAEFFVTRSYHGLEMHANSSLPEPYLVGRS 818

Query: 282  GRAWSYQDE 256
            G+A  Y+DE
Sbjct: 819  GKASGYKDE 827


>gb|EMJ13032.1| hypothetical protein PRUPE_ppa004543mg [Prunus persica]
          Length = 504

 Score =  453 bits (1165), Expect = e-124
 Identities = 241/505 (47%), Positives = 332/505 (65%), Gaps = 26/505 (5%)
 Frame = -1

Query: 1683 MELNALYDVEATATFIHTRGFKRVALQFPDELLKDSVKVVRALKTQL---------QQNS 1531
            M+L + Y++  TA FI +  F RVALQFPDELLKD+V+VV AL+ +L         Q + 
Sbjct: 1    MDLESNYEIAPTAEFIQSNKFTRVALQFPDELLKDAVRVVSALRAKLRTLRESNAEQNDD 60

Query: 1530 YDGVQMYVMADTTYGSCCVDEVAAAHVNADCVVHYGHACFSPTSKLPAWFVFRKSSFQYK 1351
               V ++VMADT YGSCCVDEV A+HV+AD VVHYGH C SPT+ LPA+FVF K+S    
Sbjct: 61   APEVGLFVMADTAYGSCCVDEVGASHVDADAVVHYGHTCLSPTTTLPAFFVFGKTSISVP 120

Query: 1350 NCIDALLKYSCSVDKPLLVLFSLEYIYAAALLKGEVEVHLKRDDMSNKIIFAELVSSEMD 1171
            +C+  L  Y+    + +LVLF L+Y ++   ++ E    +   +   ++ +A ++ S M+
Sbjct: 121  DCVKNLSHYALPNGRRVLVLFGLDYAHSIQNIR-EKFAEVCGAESKPEVQYANVICSVMN 179

Query: 1170 PS--------------SLAVNNTLSKDDTFVYQLGALKWVLHKDKKMEDYMIVWIGSESV 1033
            PS              SL  NN+        Y +G L W L +  KM+DY++ WIGS + 
Sbjct: 180  PSKGCKVSNEVQGTSGSLTDNNSFGTVSADTYSIGGLVWKLPEGHKMDDYLLFWIGSNNS 239

Query: 1032 ALTNILLTYSLCELVRYDPENSILLKDISNQERILKRRYYLVQRAKDADIVGIVIGTLGI 853
            A  NI+LTY+ CE+VRYD     ++ D+S Q +ILKRRYYL+++AKDA+IVGI++GTLG+
Sbjct: 240  AFANIVLTYNGCEIVRYDASECSMVTDLSQQRKILKRRYYLMEKAKDANIVGILVGTLGV 299

Query: 852  AGYQKVICTLKNLIKRAGKKPYSFVMGRPNPAKLANFPECNIFIYVACPETAMLDSKEFL 673
            AGY  +I  +K LI  AGKK Y+ VMGRPNPAKLANFPEC+IFIYV+C +TA+LDSKE+L
Sbjct: 300  AGYLNMIHQMKVLITGAGKKVYTLVMGRPNPAKLANFPECDIFIYVSCAQTALLDSKEYL 359

Query: 672  SPLITPYEAILAFSRGSEWTNSYSLSFSDILGSSEKEINDVDTEPHFSFIKGAYVAN-DT 496
            +P+ITP+EA+LAF+RG +WT  Y + F D++ SS  E  +   E  FSF++G YV + D 
Sbjct: 360  APVITPFEAMLAFNRGCQWTGEYVMEFRDLINSSPVESRNFSEEARFSFLQGGYVEDFDP 419

Query: 495  LSDDVDEKEEKAIALANEAKKALRLRDDSSNSGCLNITAKSGAEFLTLRSYQGLEISKET 316
              +  +E +E A+ALAN  +K L+LRD+ ++   +  TAKSGAEFL  RSYQGL+I  + 
Sbjct: 420  QGNGTEEDKEGALALANATEKVLQLRDNPNS--IVKGTAKSGAEFLATRSYQGLDIHYDG 477

Query: 315  KQSYTSIKGRSGRAWSYQDE--GHK 247
                    GRSGRA  Y+DE  GH+
Sbjct: 478  SLPEPYFIGRSGRAAGYEDEKNGHE 502


>ref|XP_006339197.1| PREDICTED: diphthamide biosynthesis protein 2-like [Solanum
            tuberosum]
          Length = 498

 Score =  452 bits (1163), Expect = e-124
 Identities = 242/498 (48%), Positives = 326/498 (65%), Gaps = 22/498 (4%)
 Frame = -1

Query: 1683 MELNALYDVEATATFIHTRGFKRVALQFPDELLKDSVKVVRALKTQLQQ---------NS 1531
            M+L +  +++  A FI  R F+RVALQFPD+LLKDS ++V AL  +L+            
Sbjct: 1    MDLESTNEIDRVAGFISARAFRRVALQFPDKLLKDSTRIVAALHEKLRSFKQLHAGSNGD 60

Query: 1530 YDGVQMYVMADTTYGSCCVDEVAAAHVNADCVVHYGHACFSPTSKLPAWFVFRKSSFQYK 1351
               V++YVMADT YG+CCVDEV A+H NADCV+HYGH CFSPTS LPA+ V  K+S    
Sbjct: 61   AKDVKLYVMADTMYGNCCVDEVGASHANADCVIHYGHTCFSPTSTLPAFLVLGKASLNVP 120

Query: 1350 NCIDALLKYSCSVDKPLLVLFSLEYIYAAALLKG--------EVEVHLKRDDMSNKIIFA 1195
             C   L +Y+    KP+LVL+ LEY +A   +K         E+EVH   D MS  I   
Sbjct: 121  LCAQTLCEYTIKAGKPILVLYGLEYTHAITEIKASVGAQVCSELEVHYA-DIMSPIITPP 179

Query: 1194 ELVSS-----EMDPSSLAVNNTLSKDDTFVYQLGALKWVLHKDKKMEDYMIVWIGSESVA 1030
            E  +S     E      A   ++ ++D   Y +G L W L   +++EDY+I ++GS+  A
Sbjct: 180  ETFNSMNEQPEQSDGQCANGCSIEENDA-AYCVGGLTWSLPVGRRIEDYLIFYVGSDDPA 238

Query: 1029 LTNILLTYSLCELVRYDPENSILLKDISNQERILKRRYYLVQRAKDADIVGIVIGTLGIA 850
              NIL+TY+ CE+VRYD     L+ D S Q+RILKRRY+L+++AKDA I+GI++GTLG+A
Sbjct: 239  FANILMTYNACEIVRYDATEDNLVNDFSQQKRILKRRYFLIEKAKDASIIGILVGTLGVA 298

Query: 849  GYQKVICTLKNLIKRAGKKPYSFVMGRPNPAKLANFPECNIFIYVACPETAMLDSKEFLS 670
            GY  +I  +K+LI RAGKK Y+FVMGRPNPAKLANFPEC+IF+YV+C +TA+LDSKEFL+
Sbjct: 299  GYLHMIHQMKDLITRAGKKAYTFVMGRPNPAKLANFPECDIFVYVSCAQTALLDSKEFLA 358

Query: 669  PLITPYEAILAFSRGSEWTNSYSLSFSDILGSSEKEINDVDTEPHFSFIKGAYVANDTLS 490
            P+ITP+EA++AF RGSEWT +Y   F D++ SS  E  +  +E  FSFI+G YV  D   
Sbjct: 359  PVITPFEAMIAFGRGSEWTGAYVTEFRDLITSSPMEAKN-QSEARFSFIQGGYV-EDVEQ 416

Query: 489  DDVDEKEEKAIALANEAKKALRLRDDSSNSGCLNITAKSGAEFLTLRSYQGLEISKETKQ 310
             +V+E E+   AL N  +KALR+RD  S +  +  TAKSGAE+   RSY GL+I  E   
Sbjct: 417  QEVEEVEDGVSALVNITEKALRVRDKDSQT-LMPGTAKSGAEYFATRSYHGLDIHPENNF 475

Query: 309  SYTSIKGRSGRAWSYQDE 256
            S   + G+SGRA  Y++E
Sbjct: 476  SEPFLIGKSGRASGYKNE 493


>ref|XP_002878279.1| diphthamide synthesis DPH2 family protein [Arabidopsis lyrata subsp.
            lyrata] gi|297324117|gb|EFH54538.1| diphthamide synthesis
            DPH2 family protein [Arabidopsis lyrata subsp. lyrata]
          Length = 493

 Score =  451 bits (1161), Expect = e-124
 Identities = 229/491 (46%), Positives = 322/491 (65%), Gaps = 15/491 (3%)
 Frame = -1

Query: 1683 MELNALYDVEATATFIHTRGFKRVALQFPDELLKDSVKVVRALKTQLQQNSYDGVQMYVM 1504
            +E  + Y++  TA F+ ++ F R+ALQFPDELLKDS KVV ALK++ +  +   ++ +VM
Sbjct: 3    LEFESKYEINRTAEFLISKSFTRIALQFPDELLKDSTKVVSALKSKTRLLTDREIRFFVM 62

Query: 1503 ADTTYGSCCVDEVAAAHVNADCVVHYGHACFSPTSKLPAWFVFRKSSFQYKNCIDALLKY 1324
            ADTTYGSCC+DEV A H++A+CVVHYG  C SPTS LPA+FVF K+S    +C+  L+ Y
Sbjct: 63   ADTTYGSCCIDEVGALHIDAECVVHYGRTCLSPTSVLPAFFVFGKASINVSSCVKHLIDY 122

Query: 1323 SCSVDKPLLVLFSLEYIYAAALLKGEVEVHLKRDDMSNKI--IFAELVSSEMDPSS-LAV 1153
            +   DKP+++L+ LEY +    ++ E+ +      +   +  +    +S   DP   +  
Sbjct: 123  ASKRDKPIMILYGLEYAHVIPHIREELGLSKTGSQLKLSVGNVLCSFISPSRDPREPMEH 182

Query: 1152 NNTLSKDDTF-------VYQLGALKWVLHKDKKMEDYMIVWIGSESVALTNILLTYSLCE 994
               LS+ D+         Y+LG L W L +  K+EDY++ WIGS+S A  N++LT++ C+
Sbjct: 183  PRPLSESDSSDSLSSSRSYRLGGLTWDLPEGSKIEDYLLFWIGSDSSAFANVVLTFNGCD 242

Query: 993  LVRYDPENSILLKDISNQERILKRRYYLVQRAKDADIVGIVIGTLGIAGYQKVICTLKNL 814
            +VRYD E   L+ +   Q RILKRRYYLV++AKDA+I+GI++GTLG+AGY  +I  ++ L
Sbjct: 243  IVRYDAEEDSLVTEFYQQRRILKRRYYLVEKAKDANIIGILVGTLGVAGYLHMIHHMQAL 302

Query: 813  IKRAGKKPYSFVMGRPNPAKLANFPECNIFIYVACPETAMLDSKEFLSPLITPYEAILAF 634
            I  AGKK Y   MGRPNPAKLANFPEC++FIY++C +TA+LDSKEF+SP+ITP+EA LAF
Sbjct: 303  ISAAGKKSYILAMGRPNPAKLANFPECDVFIYISCAQTALLDSKEFMSPVITPFEANLAF 362

Query: 633  SRGSEWTNSYSLSFSDILGS--SEKEINDVDTEPHFSFIKGAYVANDTLSDDVDEKEE-- 466
            SRGSEWT +Y + F D++ S  SE E +    EP FSF +G YV +   +D     EE  
Sbjct: 363  SRGSEWTGAYLMHFQDVINSVKSESEAHSGSEEPRFSFFQGGYVEDHKTNDQAKNGEEDT 422

Query: 465  -KAIALANEAKKALRLRDDSSNSGCLNITAKSGAEFLTLRSYQGLEISKETKQSYTSIKG 289
             + +AL   A+KAL+LR +  NS      AKSG E+   R+Y+GLEI+ E       I G
Sbjct: 423  GETMALVQAAEKALQLRGNDHNSLTKQTAAKSGPEYFLRRAYRGLEINSENTSPEPYIVG 482

Query: 288  RSGRAWSYQDE 256
            RSG+A  Y+ E
Sbjct: 483  RSGKASGYKHE 493


>ref|XP_006397104.1| hypothetical protein EUTSA_v10028620mg [Eutrema salsugineum]
            gi|557098121|gb|ESQ38557.1| hypothetical protein
            EUTSA_v10028620mg [Eutrema salsugineum]
          Length = 485

 Score =  451 bits (1160), Expect = e-124
 Identities = 231/485 (47%), Positives = 321/485 (66%), Gaps = 10/485 (2%)
 Frame = -1

Query: 1680 ELNALYDVEATATFIHTRGFKRVALQFPDELLKDSVKVVRALKTQLQQNSYDG--VQMYV 1507
            E  + Y+++ TA FI ++ F R+ALQFPDELLKDS +VV ALK + +  + D   V+ +V
Sbjct: 4    EFESRYEIDRTAEFIISKSFTRIALQFPDELLKDSTRVVSALKNKTRFLTEDDREVRFFV 63

Query: 1506 MADTTYGSCCVDEVAAAHVNADCVVHYGHACFSPTSKLPAWFVFRKSSFQYKNCIDALLK 1327
            MADTTYGSCCVDEVAA H+++DCVVHYG  C SPTS LPA+FVF K+S    +C+  LL 
Sbjct: 64   MADTTYGSCCVDEVAALHIDSDCVVHYGQTCLSPTSVLPAFFVFGKASINVSSCVKHLLD 123

Query: 1326 YSCSVDKPLLVLFSLEYIYAAALLKGEVEVHLKRDDMSNKIIFAELVSSEMDPSSLAVNN 1147
            Y+   DKP+++L+ LEY +    ++   E+ L +   S   +    +S   DP     + 
Sbjct: 124  YASKSDKPIMILYGLEYAHVIPCIR---ELGLSKLKFSVANVLCSFISPSKDPRESMEHP 180

Query: 1146 TLSKDDTFV---YQLGALKWVLHKDKKMEDYMIVWIGSESVALTNILLTYSLCELVRYDP 976
              S D+      Y+LG L W L +  K+EDY++ WIGS+S A  N++LT++ C++VRYD 
Sbjct: 181  GPSSDNLSSSRNYRLGGLTWDLPQGNKIEDYLLFWIGSDSSAFANVVLTFNGCDVVRYDA 240

Query: 975  ENSILLKDISNQERILKRRYYLVQRAKDADIVGIVIGTLGIAGYQKVICTLKNLIKRAGK 796
            E   L  + + Q +ILKRRYYLV++AKDA+I+GI++GTLG+AGY  +I  ++ LI  AGK
Sbjct: 241  EEDSLETEFTQQRKILKRRYYLVEKAKDANIIGILVGTLGVAGYLHMIHHMQALISAAGK 300

Query: 795  KPYSFVMGRPNPAKLANFPECNIFIYVACPETAMLDSKEFLSPLITPYEAILAFSRGSEW 616
            K Y   MGRPNPAKLANFPEC++FIY++C +TA+LDSKEF++P+ITP+EA LAFSRGSEW
Sbjct: 301  KSYILAMGRPNPAKLANFPECDVFIYISCAQTALLDSKEFMAPVITPFEANLAFSRGSEW 360

Query: 615  TNSYSLSFSDILGSS--EKEINDVDTEPHFSFIKGAYVANDTLSDDV---DEKEEKAIAL 451
            T +Y + F D++ SS  E E +    EP FSF +G YV     +D+    +E  E+ +AL
Sbjct: 361  TGAYLMHFQDVINSSKPEPEAHSGSEEPRFSFFQGGYVEEQDTNDEAKNGEEDTEETMAL 420

Query: 450  ANEAKKALRLRDDSSNSGCLNITAKSGAEFLTLRSYQGLEISKETKQSYTSIKGRSGRAW 271
               A++AL+LR    NS      AKSG E+   R+Y+GLEI+ +       I GRSG+A 
Sbjct: 421  VRAAERALQLRGKDHNSLTKQTAAKSGPEYFLNRAYRGLEINSDNTSPEPFIVGRSGKAS 480

Query: 270  SYQDE 256
             Y+ E
Sbjct: 481  GYKHE 485


>ref|NP_191522.1| diphthamide synthesis DPH2 family protein [Arabidopsis thaliana]
            gi|6996299|emb|CAB75460.1| putative protein [Arabidopsis
            thaliana] gi|19715609|gb|AAL91627.1| AT3g59630/T16L24_180
            [Arabidopsis thaliana] gi|53749154|gb|AAU90062.1|
            At3g59630 [Arabidopsis thaliana]
            gi|332646426|gb|AEE79947.1| diphthamide synthesis DPH2
            family protein [Arabidopsis thaliana]
          Length = 491

 Score =  451 bits (1160), Expect = e-124
 Identities = 230/489 (47%), Positives = 320/489 (65%), Gaps = 13/489 (2%)
 Frame = -1

Query: 1683 MELNALYDVEATATFIHTRGFKRVALQFPDELLKDSVKVVRALKTQLQQNSYDGVQMYVM 1504
            +E  + Y++  TA FI ++ F R+ALQFPDELLKDS KVV ALK++ +  +   V+ +VM
Sbjct: 3    LEFESKYEINRTAEFIISKSFTRIALQFPDELLKDSTKVVSALKSKTRLLTDREVRFFVM 62

Query: 1503 ADTTYGSCCVDEVAAAHVNADCVVHYGHACFSPTSKLPAWFVFRKSSFQYKNCIDALLKY 1324
            ADTTYGSCC+DEV A H++++CVVHYG  C SPTS LPA+FVF K+S    +C+  L+ Y
Sbjct: 63   ADTTYGSCCIDEVGALHIDSECVVHYGQTCLSPTSVLPAFFVFGKASINVSSCVKHLIDY 122

Query: 1323 SCSVDKPLLVLFSLEYIYAAALLKGEVEVHLKRDDMSNKIIFAELVSSEMDP-SSLAVNN 1147
            +   DKP+++L+ LEY +    ++ E+ +      +S   +    +S   DP  S+    
Sbjct: 123  ASKSDKPIMILYGLEYAHVIPQIREELGLSKTDSQLSVANVLCSFISPSKDPRESMEHPR 182

Query: 1146 TLSKDDTF-------VYQLGALKWVLHKDKKMEDYMIVWIGSESVALTNILLTYSLCELV 988
              S+ D+         Y+LG L W L +  K+EDY++ WIGS+S A  N++LT++ C++V
Sbjct: 183  PYSESDSSDSLSSSRSYRLGGLTWDLPEGSKIEDYLLFWIGSDSSAFANVVLTFNGCDIV 242

Query: 987  RYDPENSILLKDISNQERILKRRYYLVQRAKDADIVGIVIGTLGIAGYQKVICTLKNLIK 808
            RYD E   L+ +   Q RILKRRYYLV++AKDA+I+GI++GTLG+AGY  +I  ++ LI 
Sbjct: 243  RYDAEEDSLVTEFYQQRRILKRRYYLVEKAKDANIIGILVGTLGVAGYLHMIHHMQALIS 302

Query: 807  RAGKKPYSFVMGRPNPAKLANFPECNIFIYVACPETAMLDSKEFLSPLITPYEAILAFSR 628
             AGKK Y   MGRPNPAKLANFPEC++FIY++C +TA+LDSKEF+SP+ITP+EA LAFSR
Sbjct: 303  AAGKKSYILAMGRPNPAKLANFPECDVFIYISCAQTALLDSKEFMSPVITPFEANLAFSR 362

Query: 627  GSEWTNSYSLSFSDILGS--SEKEINDVDTEPHFSFIKGAYVANDTLSDDVDEKEE---K 463
            GSEWT +Y + F D++ S  SE E +    EP FSF +G YV +   +D     EE   +
Sbjct: 363  GSEWTGAYLMHFQDVINSVKSESEAHIGSEEPRFSFFQGGYVEDHKTNDQAKNGEEDTGE 422

Query: 462  AIALANEAKKALRLRDDSSNSGCLNITAKSGAEFLTLRSYQGLEISKETKQSYTSIKGRS 283
             + L   A+KAL+LR +  NS      AKSG E+   R Y+GLEI+ E       I GRS
Sbjct: 423  TMTLVQAAEKALQLRGNDHNSLTKQTAAKSGPEYFLNRVYRGLEINSENTLPEPYIVGRS 482

Query: 282  GRAWSYQDE 256
            G+A  Y+ E
Sbjct: 483  GKASGYKHE 491


>ref|XP_006290992.1| hypothetical protein CARUB_v10017106mg [Capsella rubella]
            gi|482559699|gb|EOA23890.1| hypothetical protein
            CARUB_v10017106mg [Capsella rubella]
          Length = 488

 Score =  449 bits (1154), Expect = e-123
 Identities = 228/488 (46%), Positives = 319/488 (65%), Gaps = 12/488 (2%)
 Frame = -1

Query: 1683 MELNALYDVEATATFIHTRGFKRVALQFPDELLKDSVKVVRALKTQLQQNSYDGVQMYVM 1504
            +E  + YD+  TA FI ++ F R+ALQFPDELLKDS KVV ALK++ +  +   V+ +VM
Sbjct: 3    LEFESRYDINRTAEFIISKSFTRIALQFPDELLKDSTKVVSALKSKTRLLTDREVRFFVM 62

Query: 1503 ADTTYGSCCVDEVAAAHVNADCVVHYGHACFSPTSKLPAWFVFRKSSFQYKNCIDALLKY 1324
            ADTTYGSCC+DEV A H++A+CVVHYG  C SPTS LPA+FVF K+S    +C+  L+ Y
Sbjct: 63   ADTTYGSCCIDEVGALHIDAECVVHYGQTCLSPTSALPAFFVFGKASINISSCVKHLVDY 122

Query: 1323 SCSVDKPLLVLFSLEYIYAAALLKGEVEVHLKRDDMSNKI--IFAELVSSEMDPSSLA-- 1156
            +   DKP+++L+ LEY +    ++ E+ +      +   +  +    +S   DP      
Sbjct: 123  ASKSDKPIMILYGLEYAHVIPHIREELGLSETESQLKFSVANVLCSFISPSKDPRESMEH 182

Query: 1155 ---VNNTLSKDDTFVYQLGALKWVLHKDKKMEDYMIVWIGSESVALTNILLTYSLCELVR 985
                +++LS      Y+LG L W L +  K+EDY++ WIGS++ A  N++LT++ C++VR
Sbjct: 183  PRPYSDSLSSSRN--YRLGGLTWDLLEGSKIEDYLLFWIGSDNSAFANVVLTFNGCDIVR 240

Query: 984  YDPENSILLKDISNQERILKRRYYLVQRAKDADIVGIVIGTLGIAGYQKVICTLKNLIKR 805
            YD E   L+ +   Q RIL RRYYLV++AKDA+I+GI++GTLG+AGY  +I  ++ LI  
Sbjct: 241  YDAEEDSLVTEFHQQRRILMRRYYLVEKAKDANIIGILVGTLGVAGYLHMIHHMQALISA 300

Query: 804  AGKKPYSFVMGRPNPAKLANFPECNIFIYVACPETAMLDSKEFLSPLITPYEAILAFSRG 625
            AGKK Y   MGRPNPAKLANFPEC++FIY++C +TA+LDSKEF+SP+ITP+EA LAFSRG
Sbjct: 301  AGKKSYILAMGRPNPAKLANFPECDVFIYISCAQTALLDSKEFMSPVITPFEANLAFSRG 360

Query: 624  SEWTNSYSLSFSDILGS--SEKEINDVDTEPHFSFIKGAYVANDTLSDDVDEKEEKA--- 460
            SEWT  Y + F D++ S   E E +    EP FSF +G YV +   +D     EE A   
Sbjct: 361  SEWTGEYLMQFQDVINSVKLESEAHSESEEPRFSFFQGGYVEDHKRNDQAKNGEEDAGET 420

Query: 459  IALANEAKKALRLRDDSSNSGCLNITAKSGAEFLTLRSYQGLEISKETKQSYTSIKGRSG 280
            +ALA  A+KAL+L++   NS      AKSG E+   R+Y+GLEI+ +       I GRSG
Sbjct: 421  MALARTAEKALQLKEKDHNSLTKQTAAKSGPEYFLSRAYRGLEINSDNTLPEAYIVGRSG 480

Query: 279  RAWSYQDE 256
            +A  Y+ E
Sbjct: 481  KASGYRHE 488


>ref|XP_003588543.1| Diphthamide biosynthesis protein 2 containing protein expressed
            [Medicago truncatula] gi|355477591|gb|AES58794.1|
            Diphthamide biosynthesis protein 2 containing protein
            expressed [Medicago truncatula]
          Length = 612

 Score =  446 bits (1147), Expect = e-122
 Identities = 227/509 (44%), Positives = 331/509 (65%), Gaps = 28/509 (5%)
 Frame = -1

Query: 1689 VTMELNALYDVEATATFIHTRGFKRVALQFPDELLKDSVKVVRALKTQLQQ--------- 1537
            + M+  + YD+  TA FI T  F RVALQFPD+LLKDS +VV  L+ +L+          
Sbjct: 109  LAMDFESNYDIATTADFIRTHKFTRVALQFPDDLLKDSTRVVSVLRKRLRSLRESDTTGS 168

Query: 1536 NSYDGVQMYVMADTTYGSCCVDEVAAAHVNADCVVHYGHACFSPTSKLPAWFVFRKSSFQ 1357
                 + ++VMADT YGSCCVDEV A+H+NADCV+HYGH CFSPT+ LPA+FVF K+S  
Sbjct: 169  GDEPDIGLFVMADTAYGSCCVDEVGASHINADCVIHYGHTCFSPTTTLPAFFVFGKASIG 228

Query: 1356 YKNCIDALLKYSCSVDKPLLVLFSLEYIYAA-----ALLKGEVEVHLKRDDMSNKIIFAE 1192
              +C++++ +Y+ +  KP++VL+ LEY ++      ALL+          D  +++ FA+
Sbjct: 229  LADCVESMSQYALTSSKPIMVLYGLEYAHSIQQVRKALLESST---FCTSDPKSEVHFAD 285

Query: 1191 LVSSEMDPSS--------------LAVNNTLSKDDTFVYQLGALKWVLHKDKKMEDYMIV 1054
            + S  M PS                  N++        Y +G L W L + + MEDY + 
Sbjct: 286  VPSPYMFPSKDTKKLNDLQEETCGCGDNSSSDGASGAAYSIGGLTWKLLEGQSMEDYSLF 345

Query: 1053 WIGSESVALTNILLTYSLCELVRYDPENSILLKDISNQERILKRRYYLVQRAKDADIVGI 874
            WIG ++ A  N++L ++ CE+VRYD   S ++ D+S Q RILKRRYYLV+RAKDA+IVGI
Sbjct: 346  WIGHDNSAFANVVLRFNACEIVRYDATKSQMVADLSQQRRILKRRYYLVERAKDANIVGI 405

Query: 873  VIGTLGIAGYQKVICTLKNLIKRAGKKPYSFVMGRPNPAKLANFPECNIFIYVACPETAM 694
            ++GTLG+AGY  +I  +  LI  AGKK Y+ VMG+PNPAKLANFPEC++F+YV+C +TA+
Sbjct: 406  LVGTLGVAGYLHIINQMMGLITAAGKKAYTLVMGKPNPAKLANFPECDVFLYVSCAQTAL 465

Query: 693  LDSKEFLSPLITPYEAILAFSRGSEWTNSYSLSFSDILGSSEKEINDVDTEPHFSFIKGA 514
            LDSK++L+P+ITP+EA +AF+RGS+WT +Y + F D++   + ++ + + E  FSF+KG 
Sbjct: 466  LDSKDYLAPVITPFEATIAFNRGSQWTGAYVMGFGDLINLPQVQVGNQE-EARFSFLKGG 524

Query: 513  YVANDTLSDDVDEKEEKAIALANEAKKALRLRDDSSNSGCLNITAKSGAEFLTLRSYQGL 334
            YV +    ++  E+E + +ALAN  +KAL+LRD+ ++   +   AKSGAEF   R+YQGL
Sbjct: 525  YVEDFENQENNVEEEREDLALANATEKALQLRDNCNS--IIKGGAKSGAEFFANRTYQGL 582

Query: 333  EISKETKQSYTSIKGRSGRAWSYQDEGHK 247
             I  +     + + GR GRA  Y+DE +K
Sbjct: 583  NIPDDNTTPQSFVIGRKGRAAGYEDENNK 611