BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Ephedra26_contig00018705
         (1080 letters)

Database: ./nr 
           37,332,560 sequences; 13,225,080,153 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ADX42738.1| (E)-beta-farnesene synthase 2 [Pseudotsuga menzie...   206   1e-50
sp|O81086.1|TPSD1_ABIGR RecName: Full=Alpha-bisabolene synthase;...   202   1e-49
gb|AAK83562.1| E-alpha-bisabolene synthase [Abies grandis]            202   2e-49
gb|ADX42737.1| (E)-beta-farnesene synthase 1 [Pseudotsuga menzie...   201   4e-49
gb|ADH29869.1| e-beta farnesene synthase [Pinus sylvestris]           200   7e-49
gb|AAS47689.1| E-alpha-bisabolene synthase [Picea abies]              198   4e-48
gb|AAX07265.1| (E)-beta-farnesene synthase [Pseudotsuga menziesii]    197   5e-48
sp|Q4QSN4.1|TPSBS_PSEMZ RecName: Full=(E)-gamma-bisabolene synth...   192   2e-46
gb|AAC24191.1| E-alpha-bisabolene synthase [Abies grandis]            192   3e-46
dbj|BAF43701.1| putative sesquiterpene synthase [Cycas taitungen...   191   6e-46
gb|ABW83002.1| taxadiene synthase [Taxus wallichiana var. wallic...   177   5e-42
gb|ABW82996.1| taxadiene synthase [Taxus baccata]                     177   6e-42
gb|ABW83004.1| taxadiene synthase [Taxus sumatrana]                   176   1e-41
gb|ABW82998.1| taxadiene synthase [Taxus mairei]                      176   1e-41
gb|AAZ41362.1| taxadiene synthase [Taxus mairei]                      176   1e-41
sp|Q93YA3.1|TASY_TAXBA RecName: Full=Taxadiene synthase; AltName...   176   1e-41
gb|AFJ04997.1| taxadiene synthase, partial [Taxus cuspidata]          176   2e-41
gb|AAR15329.1| taxadiene synthase [Taxus mairei]                      175   2e-41
gb|AAS18603.1| taxadiene synthase [Taxus x media]                     175   2e-41
gb|AFJ04968.1| taxadiene synthase, partial [Taxus baccata] gi|38...   175   2e-41

>gb|ADX42738.1| (E)-beta-farnesene synthase 2 [Pseudotsuga menziesii]
            gi|323364474|gb|ADX42739.1| (E)-beta-farnesene synthase 2
            [Pseudotsuga menziesii]
          Length = 825

 Score =  206 bits (525), Expect = 1e-50
 Identities = 118/279 (42%), Positives = 168/279 (60%), Gaps = 7/279 (2%)
 Frame = -3

Query: 1033 TNRAGMASERKSLHCHGNLWTYEIVHGLQSPYNDPVYKQRANKLIEEVKEDLNRIGQIQN 854
            T+ +  +  R++ + H N+W Y  V  LQSPY DP Y +R   L+ E+K  L+  G +  
Sbjct: 24   TSSSSSSLVRRTANPHPNVWDYHFVQSLQSPYTDPCYGERVETLVAEIKAMLHGEGGLM- 82

Query: 853  ISPSAYDTAWVAMVPCIDNLSSCSSFPQFPRCLQWIVSNQLQDGXXXXXXXXXXSYHVSD 674
            I+PSAYDTAWVA V  ID     S+ PQFP+ +QWI+ NQL+DG           + +SD
Sbjct: 83   ITPSAYDTAWVARVSSIDG----SARPQFPQTVQWILKNQLKDGSWGTESH----FLLSD 134

Query: 673  KLLSTLACVVALRTWHAAEDTVQKGEEYLKGGLKELNETSKEDRSMVTDFEIIFPMLLRE 494
            +LL+TL+CV+AL  W   +  VQ+G E++K  L+ + + + +D S+VTDF IIFP LLRE
Sbjct: 135  RLLATLSCVLALLKWKVGDLQVQQGIEFIKSNLEAIKDENDQD-SLVTDFNIIFPSLLRE 193

Query: 493  AKRLCLSLPYDLPCIVELSKVRERRLARRKAMITSGECFSLVCSLEGIPDLIDDWTRFIN 314
            A+ L + LPY+LP +  L   R+ RLA        G    L+ SLEGI D++ DW R ++
Sbjct: 194  AQYLDIELPYNLPYVSLLHTKRQERLANMSREEIHGVPSPLLYSLEGIEDMV-DWERIMD 252

Query: 313  VK-------CSPASTACVLIHTKDPRCLQHITELLCTHG 218
            V+        SPASTACV +HT D +CL+ +  +L   G
Sbjct: 253  VRSQDGSFLSSPASTACVFMHTGDIKCLEFLNNVLANFG 291


>sp|O81086.1|TPSD1_ABIGR RecName: Full=Alpha-bisabolene synthase; AltName:
            Full=(E)-alpha-bisabolene synthase; AltName:
            Full=AgfEabis gi|361131501|pdb|3SAE|A Chain A, Structure
            Of A Three-Domain Sesquiterpene Synthase: A Prospective
            Target For Advanced Biofuels Production
            gi|361131502|pdb|3SDQ|A Chain A, Structure Of A
            Three-Domain Sesquiterpene Synthase: A Prospective Target
            For Advanced Biofuels Production gi|361131554|pdb|3SDR|A
            Chain A, Structure Of A Three-Domain Sesquiterpene
            Synthase: A Prospective Target For Advanced Biofuels
            Production gi|361131555|pdb|3SDT|A Chain A, Structure Of
            A Three-Domain Sesquiterpene Synthase: A Prospective
            Target For Advanced Biofuels Production
            gi|361131556|pdb|3SDU|A Chain A, Structure Of A
            Three-Domain Sesquiterpene Synthase: A Prospective Target
            For Advanced Biofuels Production gi|361131583|pdb|3SDV|A
            Chain A, Structure Of A Three-Domain Sesquiterpene
            Synthase: A Prospective Target For Advanced Biofuels
            Production gi|3252840|gb|AAC24192.1| E-alpha-bisabolene
            synthase [Abies grandis]
          Length = 817

 Score =  202 bits (515), Expect = 1e-49
 Identities = 117/273 (42%), Positives = 169/273 (61%), Gaps = 10/273 (3%)
 Frame = -3

Query: 1006 RKSLHCHGNLWTYEIVHGLQSPYNDPVYKQRANKLIEEVKEDLNRI--GQIQN-ISPSAY 836
            R++ + H N+W Y++VH L+SPY D  Y++RA  L+ E+K  LN    G  ++ I+PSAY
Sbjct: 25   RRTANPHPNVWGYDLVHSLKSPYIDSSYRERAEVLVSEIKAMLNPAITGDGESMITPSAY 84

Query: 835  DTAWVAMVPCIDNLSSCSSFPQFPRCLQWIVSNQLQDGXXXXXXXXXXSYHVSDKLLSTL 656
            DTAWVA VP ID     S+ PQFP+ + WI+ NQL+DG           + +SD+LL+TL
Sbjct: 85   DTAWVARVPAIDG----SARPQFPQTVDWILKNQLKDGSWGIQSH----FLLSDRLLATL 136

Query: 655  ACVVALRTWHAAEDTVQKGEEYLKGGLKELNETSKEDRSMVTDFEIIFPMLLREAKRLCL 476
            +CV+ L  W+  +  V++G E++K  L+ + + + +D S+VTDFEIIFP LLREA+ L L
Sbjct: 137  SCVLVLLKWNVGDLQVEQGIEFIKSNLELVKDETDQD-SLVTDFEIIFPSLLREAQSLRL 195

Query: 475  SLPYDLPCIVELSKVRERRLARRKAMITSGECFSLVCSLEGIPDLIDDWTRFINVK---- 308
             LPYDLP I  L   R+ RLA+            L+ SLEGI D++ +W R + V+    
Sbjct: 196  GLPYDLPYIHLLQTKRQERLAKLSREEIYAVPSPLLYSLEGIQDIV-EWERIMEVQSQDG 254

Query: 307  ---CSPASTACVLIHTKDPRCLQHITELLCTHG 218
                SPASTACV +HT D +CL+ +  ++   G
Sbjct: 255  SFLSSPASTACVFMHTGDAKCLEFLNSVMIKFG 287


>gb|AAK83562.1| E-alpha-bisabolene synthase [Abies grandis]
          Length = 816

 Score =  202 bits (514), Expect = 2e-49
 Identities = 117/273 (42%), Positives = 169/273 (61%), Gaps = 10/273 (3%)
 Frame = -3

Query: 1006 RKSLHCHGNLWTYEIVHGLQSPYNDPVYKQRANKLIEEVKEDLNRI--GQIQN-ISPSAY 836
            R++ + H N+W Y++VH L+SPY D  Y++RA  L+ E+K  LN    G  ++ I+PSAY
Sbjct: 24   RRTANPHPNVWGYDLVHSLKSPYIDSSYRERAEVLVSEIKVMLNPAITGDGESMITPSAY 83

Query: 835  DTAWVAMVPCIDNLSSCSSFPQFPRCLQWIVSNQLQDGXXXXXXXXXXSYHVSDKLLSTL 656
            DTAWVA VP ID     S+ PQFP+ + WI+ NQL+DG           + +SD+LL+TL
Sbjct: 84   DTAWVARVPAIDG----SARPQFPQTVDWILKNQLKDGSWGIQSH----FLLSDRLLATL 135

Query: 655  ACVVALRTWHAAEDTVQKGEEYLKGGLKELNETSKEDRSMVTDFEIIFPMLLREAKRLCL 476
            +CV+ L  W+  +  V++G E++K  L+ + + + +D S+VTDFEIIFP LLREA+ L L
Sbjct: 136  SCVLVLLKWNVGDLQVEQGIEFIKSNLELVKDETDQD-SLVTDFEIIFPSLLREAQSLRL 194

Query: 475  SLPYDLPCIVELSKVRERRLARRKAMITSGECFSLVCSLEGIPDLIDDWTRFINVK---- 308
             LPYDLP I  L   R+ RLA+            L+ SLEGI D++ +W R + V+    
Sbjct: 195  GLPYDLPYIHLLQTKRQERLAKLSREEIYAVPSPLLYSLEGIQDIV-EWERIMEVQSQDG 253

Query: 307  ---CSPASTACVLIHTKDPRCLQHITELLCTHG 218
                SPASTACV +HT D +CL+ +  ++   G
Sbjct: 254  SFLSSPASTACVFMHTGDAKCLEFLNSVMIKFG 286


>gb|ADX42737.1| (E)-beta-farnesene synthase 1 [Pseudotsuga menziesii]
          Length = 825

 Score =  201 bits (511), Expect = 4e-49
 Identities = 115/270 (42%), Positives = 163/270 (60%), Gaps = 7/270 (2%)
 Frame = -3

Query: 1006 RKSLHCHGNLWTYEIVHGLQSPYNDPVYKQRANKLIEEVKEDLNRIGQIQNISPSAYDTA 827
            R++ + H N+W Y  V  LQSPY DP Y +R   L+ E+K  L+  G    I+PSAYDTA
Sbjct: 33   RRTANPHPNVWDYHFVQSLQSPYTDPCYGERVETLVAEIKAMLHGEG-CSMITPSAYDTA 91

Query: 826  WVAMVPCIDNLSSCSSFPQFPRCLQWIVSNQLQDGXXXXXXXXXXSYHVSDKLLSTLACV 647
            WVA VP ID     S+ PQFP+ ++WI+ NQL+DG           + +SD+L +TL+CV
Sbjct: 92   WVARVPSIDG----SARPQFPQTVEWILKNQLKDGSWGTESH----FLLSDRLPATLSCV 143

Query: 646  VALRTWHAAEDTVQKGEEYLKGGLKELNETSKEDRSMVTDFEIIFPMLLREAKRLCLSLP 467
            +AL  W   +  V +G E++K  L+ + + + +D S+VTDF+IIFP LLREA+ L + LP
Sbjct: 144  LALLKWKVGDLQVLQGIEFIKSTLEAIKDENDQD-SLVTDFDIIFPSLLREAQYLDIELP 202

Query: 466  YDLPCIVELSKVRERRLARRKAMITSGECFSLVCSLEGIPDLIDDWTRFINVK------- 308
            Y+LP +  L   R+ RLA        G    L+ SLEGI D++ DW R ++V+       
Sbjct: 203  YNLPYVSLLHTKRQERLANMSREEIHGVPSPLLYSLEGIEDMV-DWERIMDVRSQDGSFL 261

Query: 307  CSPASTACVLIHTKDPRCLQHITELLCTHG 218
             SPASTACV +HT D +CL+ +  +L   G
Sbjct: 262  SSPASTACVFMHTGDIKCLEFLNNVLANFG 291


>gb|ADH29869.1| e-beta farnesene synthase [Pinus sylvestris]
          Length = 811

 Score =  200 bits (509), Expect = 7e-49
 Identities = 118/270 (43%), Positives = 158/270 (58%), Gaps = 7/270 (2%)
 Frame = -3

Query: 1006 RKSLHCHGNLWTYEIVHGLQSPYNDPVYKQRANKLIEEVKEDLNRIGQIQNISPSAYDTA 827
            R++ + H N+W Y+ V  LQSPY D  Y +RA  LI E+K  L   G    I+PSAYDTA
Sbjct: 25   RRTANPHPNVWDYDFVQSLQSPYTDSCYGERAETLISEIKLVLTGEGDALMITPSAYDTA 84

Query: 826  WVAMVPCIDNLSSCSSFPQFPRCLQWIVSNQLQDGXXXXXXXXXXSYHVSDKLLSTLACV 647
            WVA VP ID     SS PQFP+ + WI+ NQL+DG           + +SD+LL+TL+CV
Sbjct: 85   WVARVPAIDG----SSRPQFPQTVDWILKNQLKDG----SWGTESHFLLSDRLLATLSCV 136

Query: 646  VALRTWHAAEDTVQKGEEYLKGGLKELNETSKEDRSMVTDFEIIFPMLLREAKRLCLSLP 467
            +AL  W      V+ G E++K  L+ + + S +D S VTDFEIIFP L+ EA+ L L LP
Sbjct: 137  LALLKWKVGHVQVEHGIEFIKSNLEAIKDESNQD-SCVTDFEIIFPSLIGEAQSLHLGLP 195

Query: 466  YDLPCIVELSKVRERRLARRKAMITSGECFSLVCSLEGIPDLIDDWTRFINVKC------ 305
            Y+LP +  L   R  +LA        G   +L+ SLEGI D + +W R + V+       
Sbjct: 196  YNLPYVRMLQMKRREKLANLPRDEIHGG--TLLSSLEGIQDTV-EWERIMEVQSQDGSFS 252

Query: 304  -SPASTACVLIHTKDPRCLQHITELLCTHG 218
             SPASTACV +HT D +CLQ +  +L   G
Sbjct: 253  GSPASTACVFMHTGDMKCLQFLNSVLTKFG 282


>gb|AAS47689.1| E-alpha-bisabolene synthase [Picea abies]
          Length = 807

 Score =  198 bits (503), Expect = 4e-48
 Identities = 118/271 (43%), Positives = 169/271 (62%), Gaps = 8/271 (2%)
 Frame = -3

Query: 1006 RKSLHCHGNLWTYEIVHGLQSPY-NDPVYKQRANKLIEEVKEDLNRIGQIQNISPSAYDT 830
            R++ + H N+W Y+ VH L+SPY +D  Y++RA  LI E+K  L   G++  ++PSAYDT
Sbjct: 21   RRTANPHPNIWGYDFVHSLKSPYTHDSSYRERAETLISEIKVMLGG-GELM-MTPSAYDT 78

Query: 829  AWVAMVPCIDNLSSCSSFPQFPRCLQWIVSNQLQDGXXXXXXXXXXSYHVSDKLLSTLAC 650
            AWVA VP ID     S+ PQFP+ ++WI+ NQL+DG           + +SD+LL+TL+C
Sbjct: 79   AWVARVPSIDG----SACPQFPQTVEWILKNQLKDGSWGTESH----FLLSDRLLATLSC 130

Query: 649  VVALRTWHAAEDTVQKGEEYLKGGLKELNETSKEDRSMVTDFEIIFPMLLREAKRLCLSL 470
            V+AL  W  A+  V++G E++K  L+ + +   +D S+VTDFEIIFP LL+EA+ L L L
Sbjct: 131  VLALLKWKVADVQVEQGIEFIKRNLQAIKDERDQD-SLVTDFEIIFPSLLKEAQSLNLGL 189

Query: 469  PYDLPCIVELSKVRERRLARRKAMITSGECFSLVCSLEGIPDLIDDWTRFINVK------ 308
            PYDLP I  L   R+ RLA        G   +L+ SLEGI D++ +W   ++V+      
Sbjct: 190  PYDLPYIRLLQTKRQERLANLSMDKIHGG--TLLSSLEGIQDIV-EWETIMDVQSQDGSF 246

Query: 307  -CSPASTACVLIHTKDPRCLQHITELLCTHG 218
              SPASTACV +HT D +CL  +  +L   G
Sbjct: 247  LSSPASTACVFMHTGDMKCLDFLNNVLTKFG 277


>gb|AAX07265.1| (E)-beta-farnesene synthase [Pseudotsuga menziesii]
          Length = 826

 Score =  197 bits (502), Expect = 5e-48
 Identities = 115/279 (41%), Positives = 163/279 (58%), Gaps = 7/279 (2%)
 Frame = -3

Query: 1033 TNRAGMASERKSLHCHGNLWTYEIVHGLQSPYNDPVYKQRANKLIEEVKEDLNRIGQIQN 854
            T+ +  +  R++ + H N+W Y  V  LQSPY DP Y +R   L+ E+K  L+  G +  
Sbjct: 25   TSSSSSSLVRRTANPHPNVWDYHFVQSLQSPYTDPCYGERVETLVAEIKAMLHGEGGLM- 83

Query: 853  ISPSAYDTAWVAMVPCIDNLSSCSSFPQFPRCLQWIVSNQLQDGXXXXXXXXXXSYHVSD 674
            I+PSAYDTAWVA VP ID     S+ PQFP+ +QWI+ NQL+DG           + +SD
Sbjct: 84   ITPSAYDTAWVARVPSIDG----SARPQFPQTVQWILKNQLKDGSWGTESH----FLLSD 135

Query: 673  KLLSTLACVVALRTWHAAEDTVQKGEEYLKGGLKELNETSKEDRSMVTDFEIIFPMLLRE 494
            +LL+TL+CV+AL  W   +  VQ+G E++K  L+ + + + ED S+VTDF+IIFP LLRE
Sbjct: 136  RLLATLSCVLALLKWKVGDLQVQQGIEFIKSNLEAIKDENDED-SLVTDFDIIFPSLLRE 194

Query: 493  AKRLCLSLPYDLPCIVELSKVRERRLARRKAMITSGECFSLVCSLEGIPDLIDDWTRFIN 314
            A+ L + LP            R+ RLA        G    L+ SLEGI D++ DW R ++
Sbjct: 195  AQYLDIELPLQPALCKSTPPKRQERLANMSREEIHGVPSPLLYSLEGIEDMV-DWERIMD 253

Query: 313  VK-------CSPASTACVLIHTKDPRCLQHITELLCTHG 218
            V+        SPAS ACV +HT D +CL+ +  +L   G
Sbjct: 254  VRSQDGSFLSSPASIACVFMHTGDIKCLEFLNNVLTNFG 292


>sp|Q4QSN4.1|TPSBS_PSEMZ RecName: Full=(E)-gamma-bisabolene synthase; Short=PmeTPS3
            gi|59799472|gb|AAX07266.1| (E)-gamma-bisabolene synthase
            [Pseudotsuga menziesii]
          Length = 815

 Score =  192 bits (488), Expect = 2e-46
 Identities = 118/274 (43%), Positives = 168/274 (61%), Gaps = 11/274 (4%)
 Frame = -3

Query: 1006 RKSLHCHGNLWTYEIVHGLQSPYN-DPVYKQRANKLIEEVKEDLNRI---GQIQNISPSA 839
            R++ + H N+W Y+++  L+SPY+ D  YK+RA+ LI E+K  L      G+ + I+PSA
Sbjct: 17   RRTANPHPNVWGYDLLCSLKSPYSRDSSYKERADTLINEIKAMLGAAFGDGK-EMITPSA 75

Query: 838  YDTAWVAMVPCIDNLSSCSSFPQFPRCLQWIVSNQLQDGXXXXXXXXXXSYHVSDKLLST 659
            YDTAWVA +P ID  SS S+ PQFP+ + WI+ NQL+DG           + +S+ LL+T
Sbjct: 76   YDTAWVARIPSIDG-SSGSARPQFPQTVDWILKNQLKDGSWGTESH----FLLSEPLLAT 130

Query: 658  LACVVALRTWHAAEDTVQKGEEYLKGGLKELNETSKEDRSMVTDFEIIFPMLLREAKRLC 479
            ++CV+AL  W   +  V++G E+LK  L+++   S +D S+VTDFEIIFP +LREA+ L 
Sbjct: 131  ISCVLALFKWQVGDLQVERGIEFLKSSLEKIKNESDQD-SLVTDFEIIFPSMLREAQSLH 189

Query: 478  LSLPYDLPCIVELSKVRERRLARRKAMITSGECFSLVCSLEGIPDLIDDWTRFINVK--- 308
            L LPYDLP I  L   R+ RLA        G    L  SLEGI D++ +W R ++++   
Sbjct: 190  LGLPYDLPYIQLLQTKRQERLANLSREKIHGGILQL-SSLEGIEDMV-EWERLMDLQSLD 247

Query: 307  ----CSPASTACVLIHTKDPRCLQHITELLCTHG 218
                 SPASTA V IHT D +CL  +  +L   G
Sbjct: 248  GSFLSSPASTAFVFIHTGDLKCLAFLNSVLAKFG 281


>gb|AAC24191.1| E-alpha-bisabolene synthase [Abies grandis]
          Length = 782

 Score =  192 bits (487), Expect = 3e-46
 Identities = 113/261 (43%), Positives = 161/261 (61%), Gaps = 10/261 (3%)
 Frame = -3

Query: 970 YEIVHGLQSPYNDPVYKQRANKLIEEVKEDLNRI--GQIQN-ISPSAYDTAWVAMVPCID 800
           Y++VH L+SPY D  Y++RA  L+ E+K  LN    G  ++ I+PSAYDTAWVA VP ID
Sbjct: 2   YDLVHSLKSPYIDSSYRERAEVLVSEIKVMLNPAITGDGESMITPSAYDTAWVARVPAID 61

Query: 799 NLSSCSSFPQFPRCLQWIVSNQLQDGXXXXXXXXXXSYHVSDKLLSTLACVVALRTWHAA 620
                S+ PQFP+ + WI+ NQL+DG           + +SD+LL+TL+CV+ L  W+  
Sbjct: 62  G----SARPQFPQTVDWILKNQLKDGSWGIQSH----FLLSDRLLATLSCVLVLLKWNVG 113

Query: 619 EDTVQKGEEYLKGGLKELNETSKEDRSMVTDFEIIFPMLLREAKRLCLSLPYDLPCIVEL 440
           +  V++G E++K  L+ + + + +D S+VTDFEIIFP LLREA+ L L LPYDLP I  L
Sbjct: 114 DLQVEQGIEFIKSNLELVKDETDQD-SLVTDFEIIFPSLLREAQSLRLGLPYDLPYIHLL 172

Query: 439 SKVRERRLARRKAMITSGECFSLVCSLEGIPDLIDDWTRFINVK-------CSPASTACV 281
              R+ RLA+            L+ SLEGI D++ +W R + V+        SPASTACV
Sbjct: 173 QTKRQERLAKLSREEIYAVPSPLLYSLEGIQDIV-EWERIMEVQSQDGSFLSSPASTACV 231

Query: 280 LIHTKDPRCLQHITELLCTHG 218
            +HT D +CL+ +  ++   G
Sbjct: 232 FMHTGDAKCLEFLNSVMIKFG 252


>dbj|BAF43701.1| putative sesquiterpene synthase [Cycas taitungensis]
          Length = 846

 Score =  191 bits (484), Expect = 6e-46
 Identities = 111/273 (40%), Positives = 161/273 (58%), Gaps = 7/273 (2%)
 Frame = -3

Query: 1015 ASERKSLHCHGNLWTYEIVHGLQSPYNDPVYKQRANKLIEEVKEDLNRIGQIQNISPSAY 836
            A  R++ + H NLW+ + +H L+SPY++  Y++RA  LI E+K   + +G  + ISPS Y
Sbjct: 56   AVSRRTANYHPNLWSSDFIHSLKSPYSECCYRERAETLISEIKGMFDAMGDGE-ISPSPY 114

Query: 835  DTAWVAMVPCIDNLSSCSSFPQFPRCLQWIVSNQLQDGXXXXXXXXXXSYHVSDKLLSTL 656
            DTAWVA V  +D +      PQFP+ L WI+ NQL DG           + +SD+LL+TL
Sbjct: 115  DTAWVAKVEAVDGVFR----PQFPQALDWILQNQLDDGSWGTPSH----FLLSDRLLATL 166

Query: 655  ACVVALRTWHAAEDTVQKGEEYLKGGLKELNETSKEDRSMVTDFEIIFPMLLREAKRLCL 476
            +CV++L  W      VQ+G E++   L+ +N+ S++  S+V D E IF  LL EAK L L
Sbjct: 167  SCVLSLTKWKKGHPQVQRGLEFITNTLRLMNDESQD--SLVADLEFIFSSLLNEAKSLEL 224

Query: 475  SLPYDLPCIVELSKVRERRLARRKAMITSGECFSLVCSLEGIPDLIDDWTRFINVK---- 308
            SLPYDLPC+ +L + R+ RLAR           SL+  LEG+ D+I  W R ++V+    
Sbjct: 225  SLPYDLPCMDQLFRKRQERLARISIDSFHSLPSSLLYCLEGLQDII-GWKRIMDVQNKDG 283

Query: 307  ---CSPASTACVLIHTKDPRCLQHITELLCTHG 218
                SPA+TA V +HT D +CL  +  +L   G
Sbjct: 284  SFLSSPAATAFVYMHTGDQKCLAFLNRVLAKFG 316


>gb|ABW83002.1| taxadiene synthase [Taxus wallichiana var. wallichiana]
          Length = 343

 Score =  177 bits (450), Expect = 5e-42
 Identities = 105/272 (38%), Positives = 161/272 (59%), Gaps = 8/272 (2%)
 Frame = -3

Query: 1006 RKSLHCHGNLWTYEIVHGLQSPYNDP-VYKQRANKLIEEVKEDLNRIGQIQNISPSAYDT 830
            R S + HG+LW + ++  L++P+ +   Y++RA++L+ ++K+  N +G   +ISPSAYDT
Sbjct: 84   RLSANYHGDLWHHNVIQTLETPFRESSTYQERADELVVKIKDMFNALGD-GDISPSAYDT 142

Query: 829  AWVAMVPCIDNLSSCSSFPQFPRCLQWIVSNQLQDGXXXXXXXXXXSYHVSDKLLSTLAC 650
            AWVA V  I   S  S  P+FP+ L W+++NQLQDG           + + D+LL+T+  
Sbjct: 143  AWVARVATIS--SDGSEKPRFPQALNWVLNNQLQDGSWGIESH----FSLCDRLLNTINS 196

Query: 649  VVALRTWHAAEDTVQKGEEYLKGGLKELNETSKEDRSMVTDFEIIFPMLLREAKRLCLSL 470
            V+AL  W      V++G E++   L+ LNE  +    +  DFEIIFP LL++AK L ++L
Sbjct: 197  VIALSVWKTGHSQVEQGTEFIAENLRLLNEEDE----LSPDFEIIFPALLQKAKALGINL 252

Query: 469  PYDLPCIVELSKVRERRLARRKAMITSGECFSLVCSLEGIPDLIDDWTRFINVK------ 308
            PYDLP I  LS  RE RL    A   +    +++ +LEG+ ++I DW + +  +      
Sbjct: 253  PYDLPFIKSLSTTREARLTDVSAAADNIPA-NMLNALEGLEEVI-DWNKIMRFQSKDGSF 310

Query: 307  -CSPASTACVLIHTKDPRCLQHITELLCTHGG 215
              SPASTACVL++T D +C   +  LL   GG
Sbjct: 311  LSSPASTACVLMNTGDEKCFTFLNNLLDKFGG 342


>gb|ABW82996.1| taxadiene synthase [Taxus baccata]
          Length = 343

 Score =  177 bits (449), Expect = 6e-42
 Identities = 104/272 (38%), Positives = 162/272 (59%), Gaps = 8/272 (2%)
 Frame = -3

Query: 1006 RKSLHCHGNLWTYEIVHGLQSPYNDP-VYKQRANKLIEEVKEDLNRIGQIQNISPSAYDT 830
            R S + HG+LW + ++  L++P+ +   +++RA++L+E++K+  N +G   +ISPSAYDT
Sbjct: 84   RLSANYHGDLWHHNVIQTLETPFRESSTFQERADELVEKIKDMFNALGD-GDISPSAYDT 142

Query: 829  AWVAMVPCIDNLSSCSSFPQFPRCLQWIVSNQLQDGXXXXXXXXXXSYHVSDKLLSTLAC 650
            AWVA V  I   S  S  P+FP+ L W+++NQLQDG           + + D+LL+T+  
Sbjct: 143  AWVARVATIS--SDGSEKPRFPQALNWVLNNQLQDGSWGIESH----FSLCDRLLNTVNS 196

Query: 649  VVALRTWHAAEDTVQKGEEYLKGGLKELNETSKEDRSMVTDFEIIFPMLLREAKRLCLSL 470
            V+AL  W      V++G E++   L+ LNE  +    +  DFEIIFP LL++AK L ++L
Sbjct: 197  VIALSVWKTGHSQVEQGTEFIAENLRLLNEEDE----LSPDFEIIFPALLQKAKALGINL 252

Query: 469  PYDLPCIVELSKVRERRLARRKAMITSGECFSLVCSLEGIPDLIDDWTRFINVK------ 308
            PYDLP I  LS  RE RL    A   +    +++ +LEG+ ++I DW + +  +      
Sbjct: 253  PYDLPLIKSLSTTREARLTDVSAAADNIPA-NMLNALEGLEEVI-DWNKIMRFQSKDGSF 310

Query: 307  -CSPASTACVLIHTKDPRCLQHITELLCTHGG 215
              SPASTACVL++T + +C   +  LL   GG
Sbjct: 311  LSSPASTACVLMNTGEEKCFTFLNNLLDKFGG 342


>gb|ABW83004.1| taxadiene synthase [Taxus sumatrana]
          Length = 343

 Score =  176 bits (447), Expect = 1e-41
 Identities = 105/272 (38%), Positives = 161/272 (59%), Gaps = 8/272 (2%)
 Frame = -3

Query: 1006 RKSLHCHGNLWTYEIVHGLQSPYNDP-VYKQRANKLIEEVKEDLNRIGQIQNISPSAYDT 830
            R S + HG+LW + ++  L++P+ +   Y++RA++L+ ++K+  N +G   +ISPSAYDT
Sbjct: 84   RLSANYHGDLWHHNVIQTLETPFRESSTYQERADELVVKIKDMFNALGD-GDISPSAYDT 142

Query: 829  AWVAMVPCIDNLSSCSSFPQFPRCLQWIVSNQLQDGXXXXXXXXXXSYHVSDKLLSTLAC 650
            AWVA V  I   S  S  P+FP+ L W+++NQLQDG           + + D+LL+T+  
Sbjct: 143  AWVARVATIS--SDGSEKPRFPQALNWVLNNQLQDGSWGIESH----FSLCDRLLNTINS 196

Query: 649  VVALRTWHAAEDTVQKGEEYLKGGLKELNETSKEDRSMVTDFEIIFPMLLREAKRLCLSL 470
            V+AL  W      V++G E++   L+ LNE  +    +  DFEIIFP LL++AK L ++L
Sbjct: 197  VIALSVWKTGHSQVEQGTEFIAENLRLLNEEDE----LSPDFEIIFPALLQKAKALGINL 252

Query: 469  PYDLPCIVELSKVRERRLARRKAMITSGECFSLVCSLEGIPDLIDDWTRFINVK------ 308
            PYDLP I  LS  RE RL    A   +    +++ +LEG+ ++I DW + +  +      
Sbjct: 253  PYDLPFIKYLSTTREARLTDVSAAADNIPA-NMLNALEGLEEVI-DWKKIMRFQSKDGSF 310

Query: 307  -CSPASTACVLIHTKDPRCLQHITELLCTHGG 215
              SPASTACVL++T D +C   +  LL   GG
Sbjct: 311  LSSPASTACVLMNTGDEKCFTFLNNLLDKFGG 342


>gb|ABW82998.1| taxadiene synthase [Taxus mairei]
          Length = 343

 Score =  176 bits (447), Expect = 1e-41
 Identities = 105/272 (38%), Positives = 161/272 (59%), Gaps = 8/272 (2%)
 Frame = -3

Query: 1006 RKSLHCHGNLWTYEIVHGLQSPYNDP-VYKQRANKLIEEVKEDLNRIGQIQNISPSAYDT 830
            R S + HG+LW + ++  L++P+ +   Y++RA++L+ ++K+  N +G   +ISPSAYDT
Sbjct: 84   RLSANYHGDLWHHNVIQTLETPFRESSTYQERADELVVKIKDMFNALGD-GDISPSAYDT 142

Query: 829  AWVAMVPCIDNLSSCSSFPQFPRCLQWIVSNQLQDGXXXXXXXXXXSYHVSDKLLSTLAC 650
            AWVA V  I   S  S  P+FP+ L W+++NQLQDG           + + D+LL+T+  
Sbjct: 143  AWVARVATIS--SDGSEKPRFPQALNWVLNNQLQDGSWGIESH----FSLCDRLLNTINS 196

Query: 649  VVALRTWHAAEDTVQKGEEYLKGGLKELNETSKEDRSMVTDFEIIFPMLLREAKRLCLSL 470
            V+AL  W      V++G E++   L+ LNE  +    +  DFEIIFP LL++AK L ++L
Sbjct: 197  VIALSVWKTGHSQVEQGTEFIAENLRLLNEEDE----LSPDFEIIFPALLQKAKSLGINL 252

Query: 469  PYDLPCIVELSKVRERRLARRKAMITSGECFSLVCSLEGIPDLIDDWTRFINVK------ 308
            PYDLP I  LS  RE RL    A   +    +++ +LEG+ ++I DW + +  +      
Sbjct: 253  PYDLPFIKYLSTTREARLTDVSAAADNIPA-NMLNALEGLEEVI-DWKKIMRFQSKDGSF 310

Query: 307  -CSPASTACVLIHTKDPRCLQHITELLCTHGG 215
              SPASTACVL++T D +C   +  LL   GG
Sbjct: 311  LSSPASTACVLMNTGDEKCFTFLNNLLDKFGG 342


>gb|AAZ41362.1| taxadiene synthase [Taxus mairei]
          Length = 862

 Score =  176 bits (447), Expect = 1e-41
 Identities = 105/272 (38%), Positives = 161/272 (59%), Gaps = 8/272 (2%)
 Frame = -3

Query: 1006 RKSLHCHGNLWTYEIVHGLQSPYNDP-VYKQRANKLIEEVKEDLNRIGQIQNISPSAYDT 830
            R S + HG+LW + ++  L++P+ +   Y++RA++L+ ++K+  N +G   +ISPSAYDT
Sbjct: 84   RLSANYHGDLWHHNVIQTLETPFRESSTYQERADELVVKIKDMFNALGD-GDISPSAYDT 142

Query: 829  AWVAMVPCIDNLSSCSSFPQFPRCLQWIVSNQLQDGXXXXXXXXXXSYHVSDKLLSTLAC 650
            AWVA V  I   S  S  P+FP+ L W+++NQLQDG           + + D+LL+T+  
Sbjct: 143  AWVARVATIS--SDGSEKPRFPQALNWVLNNQLQDGSWGIESH----FSLCDRLLNTINS 196

Query: 649  VVALRTWHAAEDTVQKGEEYLKGGLKELNETSKEDRSMVTDFEIIFPMLLREAKRLCLSL 470
            V+AL  W      V++G E++   L+ LNE  +    +  DFEIIFP LL++AK L ++L
Sbjct: 197  VIALSVWKTGHSQVEQGTEFIAENLRLLNEEDE----LSPDFEIIFPALLQKAKSLGINL 252

Query: 469  PYDLPCIVELSKVRERRLARRKAMITSGECFSLVCSLEGIPDLIDDWTRFINVK------ 308
            PYDLP I  LS  RE RL    A   +    +++ +LEG+ ++I DW + +  +      
Sbjct: 253  PYDLPFIKYLSTTREARLTDVSAAADNIPA-NMLNALEGLEEVI-DWKKIMRFQSKDGSS 310

Query: 307  -CSPASTACVLIHTKDPRCLQHITELLCTHGG 215
              SPASTACVL++T D +C   +  LL   GG
Sbjct: 311  LSSPASTACVLMNTGDEKCFTFLNNLLDKFGG 342


>sp|Q93YA3.1|TASY_TAXBA RecName: Full=Taxadiene synthase; AltName:
            Full=Taxa-4(5),11(12)-diene synthase
            gi|14571793|emb|CAC42773.1| taxadiene synthase [Taxus
            baccata]
          Length = 862

 Score =  176 bits (446), Expect = 1e-41
 Identities = 104/272 (38%), Positives = 161/272 (59%), Gaps = 8/272 (2%)
 Frame = -3

Query: 1006 RKSLHCHGNLWTYEIVHGLQSPYNDP-VYKQRANKLIEEVKEDLNRIGQIQNISPSAYDT 830
            R S + HG+LW + ++  L++P+ +   Y++RA++L+ ++K+  N +G   +ISPSAYDT
Sbjct: 84   RLSANYHGDLWHHNVIQTLETPFRESSTYQERADELVVKIKDMFNALGD-GDISPSAYDT 142

Query: 829  AWVAMVPCIDNLSSCSSFPQFPRCLQWIVSNQLQDGXXXXXXXXXXSYHVSDKLLSTLAC 650
            AWVA V  +   S  S  P+FP+ L W+++NQLQDG           + + D+LL+T+  
Sbjct: 143  AWVARVATVS--SDGSEKPRFPQALNWVLNNQLQDGSWGIESH----FSLCDRLLNTVNS 196

Query: 649  VVALRTWHAAEDTVQKGEEYLKGGLKELNETSKEDRSMVTDFEIIFPMLLREAKRLCLSL 470
            V+AL  W      V++G E++   L+ LNE  +    +  DFEIIFP LL++AK L ++L
Sbjct: 197  VIALSVWKTGHSQVEQGAEFIAENLRLLNEEDE----LSPDFEIIFPALLQKAKALGINL 252

Query: 469  PYDLPCIVELSKVRERRLARRKAMITSGECFSLVCSLEGIPDLIDDWTRFINVK------ 308
            PYDLP I  LS  RE RL    A   +    +++ +LEG+ ++I DW + +  +      
Sbjct: 253  PYDLPFIKSLSTTREARLTDVSAAADNIPA-NMLNALEGLEEVI-DWNKIMRFQSKDGSF 310

Query: 307  -CSPASTACVLIHTKDPRCLQHITELLCTHGG 215
              SPASTACVL++T D +C   +  LL   GG
Sbjct: 311  LSSPASTACVLMNTGDEKCFTLLNNLLDKFGG 342


>gb|AFJ04997.1| taxadiene synthase, partial [Taxus cuspidata]
          Length = 789

 Score =  176 bits (445), Expect = 2e-41
 Identities = 105/272 (38%), Positives = 159/272 (58%), Gaps = 8/272 (2%)
 Frame = -3

Query: 1006 RKSLHCHGNLWTYEIVHGLQSPYNDP-VYKQRANKLIEEVKEDLNRIGQIQNISPSAYDT 830
            R S + HG+LW + ++  L++P+ +   Y++RA++L+ ++K+  N +G   +ISPSAYDT
Sbjct: 44   RLSANYHGDLWHHNVIQTLETPFRESSTYQERADELVVKIKDMFNALGD-GDISPSAYDT 102

Query: 829  AWVAMVPCIDNLSSCSSFPQFPRCLQWIVSNQLQDGXXXXXXXXXXSYHVSDKLLSTLAC 650
            AWVA V  I   S  S  P+FP+ L W+ +NQLQDG           + + D+LL+T   
Sbjct: 103  AWVARVATIS--SDGSEKPRFPQALNWVFNNQLQDGSWGIESH----FSLCDRLLNTTNS 156

Query: 649  VVALRTWHAAEDTVQKGEEYLKGGLKELNETSKEDRSMVTDFEIIFPMLLREAKRLCLSL 470
            V+AL  W      V++G E++   L+ LNE  +    +  DFEIIFP LL++AK L ++L
Sbjct: 157  VIALSVWKTGHSQVEQGTEFIAENLRLLNEEDE----LSPDFEIIFPALLQKAKALGINL 212

Query: 469  PYDLPCIVELSKVRERRLARRKAMITSGECFSLVCSLEGIPDLIDDWTRFINVK------ 308
            PYDLP I  LS  RE RL    A   +    +++ +LEG+ ++I DW + +  +      
Sbjct: 213  PYDLPFIKSLSTTREARLTDVSAAADNIPA-NMLNALEGLEEVI-DWNKIMRFQSKDGSF 270

Query: 307  -CSPASTACVLIHTKDPRCLQHITELLCTHGG 215
              SPASTACVL++T D +C   +  LL   GG
Sbjct: 271  LSSPASTACVLMNTGDEKCFTFLNNLLDKFGG 302


>gb|AAR15329.1| taxadiene synthase [Taxus mairei]
          Length = 862

 Score =  175 bits (444), Expect = 2e-41
 Identities = 104/272 (38%), Positives = 160/272 (58%), Gaps = 8/272 (2%)
 Frame = -3

Query: 1006 RKSLHCHGNLWTYEIVHGLQSPYNDP-VYKQRANKLIEEVKEDLNRIGQIQNISPSAYDT 830
            R S + HG+LW + ++  L++P+ +   Y++RA++L+ ++K+  N +G   +ISPSAYDT
Sbjct: 84   RLSANYHGDLWHHNVIQTLETPFRESSTYQERADELVVKIKDMFNALGD-GDISPSAYDT 142

Query: 829  AWVAMVPCIDNLSSCSSFPQFPRCLQWIVSNQLQDGXXXXXXXXXXSYHVSDKLLSTLAC 650
            AWVA V  I   S  S  P+FP+ L W+++NQLQDG           + + D+LL+T+  
Sbjct: 143  AWVARVATIS--SDGSEKPRFPQALNWVLNNQLQDGSWGIESH----FSLCDRLLNTINS 196

Query: 649  VVALRTWHAAEDTVQKGEEYLKGGLKELNETSKEDRSMVTDFEIIFPMLLREAKRLCLSL 470
            V+ L  W      V++G E++   L+ LNE  +    +  DFEIIFP LL++AK L ++L
Sbjct: 197  VIVLSVWKTGHSQVEQGTEFIAENLRLLNEEDE----LSPDFEIIFPALLQKAKALGINL 252

Query: 469  PYDLPCIVELSKVRERRLARRKAMITSGECFSLVCSLEGIPDLIDDWTRFINVK------ 308
            PYDLP I  LS  RE RL    A   +    +++ +LEG+ ++I DW + +  +      
Sbjct: 253  PYDLPFIKYLSTTREARLTDVSAAADNIPA-NMLNALEGLEEVI-DWKKIMRFRSKDGSF 310

Query: 307  -CSPASTACVLIHTKDPRCLQHITELLCTHGG 215
              SPASTACVL++T D +C   +  LL   GG
Sbjct: 311  LSSPASTACVLMNTGDEKCFTFLNNLLDKFGG 342


>gb|AAS18603.1| taxadiene synthase [Taxus x media]
          Length = 862

 Score =  175 bits (444), Expect = 2e-41
 Identities = 103/272 (37%), Positives = 161/272 (59%), Gaps = 8/272 (2%)
 Frame = -3

Query: 1006 RKSLHCHGNLWTYEIVHGLQSPYNDP-VYKQRANKLIEEVKEDLNRIGQIQNISPSAYDT 830
            R S + HG+LW + ++  L++P+ +   +++RA++L+ ++K+  N +G   +ISPSAYDT
Sbjct: 84   RLSANYHGDLWHHNVIQTLETPFRESSTFQERADELVVKIKDMFNALGD-GDISPSAYDT 142

Query: 829  AWVAMVPCIDNLSSCSSFPQFPRCLQWIVSNQLQDGXXXXXXXXXXSYHVSDKLLSTLAC 650
            AWVA V  +   S  S  P+FP+ L W+++NQLQDG           + + D+LL+T+  
Sbjct: 143  AWVARVATVS--SDGSEKPRFPQALNWVLNNQLQDGSWGIESH----FSLCDRLLNTVNS 196

Query: 649  VVALRTWHAAEDTVQKGEEYLKGGLKELNETSKEDRSMVTDFEIIFPMLLREAKRLCLSL 470
            V+AL  W      V++G E++   L+ LNE  +    +  DFEIIFP LL++AK L ++L
Sbjct: 197  VIALSVWKTGHSQVEQGTEFIAENLRLLNEEDE----LSPDFEIIFPALLQKAKALGINL 252

Query: 469  PYDLPCIVELSKVRERRLARRKAMITSGECFSLVCSLEGIPDLIDDWTRFINVK------ 308
            PYDLP I  LS  RE RL    A +      +++ +LEG+ ++I DW + +  +      
Sbjct: 253  PYDLPFIKSLSTTREARLTDVSA-VADNIPANMLNALEGLEEVI-DWNKIMRFQSKDGSF 310

Query: 307  -CSPASTACVLIHTKDPRCLQHITELLCTHGG 215
              SPASTACVL++T D +C   +  LL   GG
Sbjct: 311  LSSPASTACVLMNTGDEKCFTLLNNLLDKFGG 342


>gb|AFJ04968.1| taxadiene synthase, partial [Taxus baccata]
            gi|386304904|gb|AFJ04976.1| taxadiene synthase, partial
            [Taxus baccata] gi|386304922|gb|AFJ04985.1| taxadiene
            synthase, partial [Taxus baccata]
            gi|386304934|gb|AFJ04991.1| taxadiene synthase, partial
            [Taxus baccata] gi|386304936|gb|AFJ04992.1| taxadiene
            synthase, partial [Taxus baccata]
            gi|386304942|gb|AFJ04995.1| taxadiene synthase, partial
            [Taxus baccata] gi|386304944|gb|AFJ04996.1| taxadiene
            synthase, partial [Taxus baccata]
            gi|386304948|gb|AFJ04998.1| taxadiene synthase, partial
            [Taxus baccata] gi|386304950|gb|AFJ04999.1| taxadiene
            synthase, partial [Taxus baccata]
            gi|386304954|gb|AFJ05001.1| taxadiene synthase, partial
            [Taxus baccata]
          Length = 789

 Score =  175 bits (444), Expect = 2e-41
 Identities = 103/272 (37%), Positives = 161/272 (59%), Gaps = 8/272 (2%)
 Frame = -3

Query: 1006 RKSLHCHGNLWTYEIVHGLQSPYNDP-VYKQRANKLIEEVKEDLNRIGQIQNISPSAYDT 830
            R S + HG+LW + ++  L++P+ +   +++RA++L+ ++K+  N +G   +ISPSAYDT
Sbjct: 44   RLSANYHGDLWHHNVIQTLETPFRESSTFQERADELVVKIKDMFNALGD-GDISPSAYDT 102

Query: 829  AWVAMVPCIDNLSSCSSFPQFPRCLQWIVSNQLQDGXXXXXXXXXXSYHVSDKLLSTLAC 650
            AWVA V  +   S  S  P+FP+ L W+++NQLQDG           + + D+LL+T+  
Sbjct: 103  AWVARVATVS--SDGSEKPRFPQALNWVLNNQLQDGSWGIESH----FSLCDRLLNTVNS 156

Query: 649  VVALRTWHAAEDTVQKGEEYLKGGLKELNETSKEDRSMVTDFEIIFPMLLREAKRLCLSL 470
            V+AL  W      V++G E++   L+ LNE  +    +  DFEIIFP LL++AK L ++L
Sbjct: 157  VIALSVWKTGHSQVEQGTEFIAENLRLLNEEDE----LSPDFEIIFPALLQKAKALGINL 212

Query: 469  PYDLPCIVELSKVRERRLARRKAMITSGECFSLVCSLEGIPDLIDDWTRFINVK------ 308
            PYDLP I  LS  RE RL    A +      +++ +LEG+ ++I DW + +  +      
Sbjct: 213  PYDLPFIKSLSTTREARLTDVSA-VADNIPANMLNALEGLEEVI-DWNKIMRFQSKDGSF 270

Query: 307  -CSPASTACVLIHTKDPRCLQHITELLCTHGG 215
              SPASTACVL++T D +C   +  LL   GG
Sbjct: 271  LSSPASTACVLMNTGDEKCFTLLNNLLDKFGG 302