BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Ephedra26_contig00017166
         (832 letters)

Database: ./nr 
           37,332,560 sequences; 13,225,080,153 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ABR18355.1| unknown [Picea sitchensis]                             393   e-107
ref|XP_004517049.1| PREDICTED: purple acid phosphatase 18-like i...   379   e-103
ref|XP_006352507.1| PREDICTED: purple acid phosphatase 18-like [...   378   e-102
ref|XP_004248333.1| PREDICTED: purple acid phosphatase 18-like [...   377   e-102
ref|XP_006851491.1| hypothetical protein AMTR_s00040p00145560 [A...   375   e-101
ref|XP_004303000.1| PREDICTED: purple acid phosphatase 22-like [...   374   e-101
ref|XP_003617509.1| Purple acid phosphatase [Medicago truncatula...   374   e-101
ref|XP_003625794.1| Purple acid phosphatase [Medicago truncatula...   374   e-101
ref|XP_006588840.1| PREDICTED: purple acid phosphatase 22-like [...   374   e-101
ref|XP_002278488.2| PREDICTED: purple acid phosphatase 18-like [...   373   e-101
ref|XP_006858168.1| hypothetical protein AMTR_s00062p00146590 [A...   372   e-101
ref|XP_004517048.1| PREDICTED: purple acid phosphatase 18-like i...   372   e-101
ref|XP_004500050.1| PREDICTED: purple acid phosphatase 18-like [...   372   e-101
ref|NP_001150058.1| LOC100283685 precursor [Zea mays] gi|1956363...   372   e-100
ref|XP_003600057.1| Purple acid phosphatase [Medicago truncatula...   371   e-100
ref|XP_002317591.1| calcineurin-like phosphoesterase family prot...   371   e-100
gb|ADM32502.1| purple acid phosphatases [Glycine max]                 370   e-100
gb|ACG42188.1| purple acid phosphatase precursor [Zea mays]           370   e-100
ref|XP_003521454.2| PREDICTED: purple acid phosphatase 22 [Glyci...   370   e-100
ref|XP_004982542.1| PREDICTED: purple acid phosphatase 18-like [...   370   e-100

>gb|ABR18355.1| unknown [Picea sitchensis]
          Length = 424

 Score =  393 bits (1009), Expect = e-107
 Identities = 178/267 (66%), Positives = 217/267 (81%), Gaps = 1/267 (0%)
 Frame = +1

Query: 1   TKWTRKTLNKVNNNPYDVLLLPGDLAYADYLQFLWDSFGRMIQPLASSRPWMVTQGNHEI 180
           T WT  TL  V    YDVLLLPGDL+YADY Q LWDSFGR+++PLASSRPWMVTQGNHEI
Sbjct: 151 TGWTTSTLQHVQQMNYDVLLLPGDLSYADYRQPLWDSFGRLVEPLASSRPWMVTQGNHEI 210

Query: 181 ENL-LFIEKPFIAYNSRWKMPFAESSSPSNLFYSFEVSGAHILMLGSYANFSRDSEQFAW 357
           E + L +  PF AYN+RWKMP+ ES SPSNL+YSFEV+GAHILMLGSYA F  DS+Q+ W
Sbjct: 211 EKIPLLVSTPFKAYNARWKMPYQESGSPSNLYYSFEVAGAHILMLGSYAEFGTDSDQYKW 270

Query: 358 LQGDLAKMDRTRTPWLIALMHVPWYSTNKAHKGEGDDMKDAMEEILYQAKVDLVLVGHVH 537
           LQGDL++++R +TPWLIAL+H PWY+TN AH+GEGDDMKDAMEE+L+ AKVD+V  GHVH
Sbjct: 271 LQGDLSRVNRRKTPWLIALIHAPWYNTNTAHQGEGDDMKDAMEELLHAAKVDIVFAGHVH 330

Query: 538 AYERFNRVYNDKADACGPVHITVGDGGNREGLAKRFEDPQSPLSMFREASYGYGELYLAN 717
           AYERF RV+ ++ + CG VHIT+GDGGNREGLA R+EDP S LS FREAS+G+GE  + N
Sbjct: 331 AYERFTRVFKNQPNPCGSVHITIGDGGNREGLASRYEDPPSGLSEFREASFGHGEFVIYN 390

Query: 718 ATHALWSWRRTTDVDNSAADHVWLQNL 798
           ATHA W+W +  D ++  +D VW+ +L
Sbjct: 391 ATHAHWTWHQNDDDESVVSDEVWINSL 417


>ref|XP_004517049.1| PREDICTED: purple acid phosphatase 18-like isoform X2 [Cicer
           arietinum]
          Length = 378

 Score =  379 bits (973), Expect = e-103
 Identities = 168/271 (61%), Positives = 211/271 (77%)
 Frame = +1

Query: 1   TKWTRKTLNKVNNNPYDVLLLPGDLAYADYLQFLWDSFGRMIQPLASSRPWMVTQGNHEI 180
           T WT+ TL+ ++   YDV LLPGDL+YAD +Q LWDSFGR+++PLAS+RPWMVT+GNHE 
Sbjct: 93  TGWTKSTLDHIDQCKYDVYLLPGDLSYADCMQHLWDSFGRLVEPLASARPWMVTEGNHEE 152

Query: 181 ENLLFIEKPFIAYNSRWKMPFAESSSPSNLFYSFEVSGAHILMLGSYANFSRDSEQFAWL 360
           EN+LF+   F++YNSRWKMPF ES S SNL+YSFEV+G H++MLGSYA++   SEQ+ WL
Sbjct: 153 ENILFLTDEFVSYNSRWKMPFEESGSTSNLYYSFEVAGVHVIMLGSYADYDESSEQYTWL 212

Query: 361 QGDLAKMDRTRTPWLIALMHVPWYSTNKAHKGEGDDMKDAMEEILYQAKVDLVLVGHVHA 540
             DL+K+DRTRTPWL+ L HVPWY++NKAH+G GDDM   ME +LY A VDLV+ GHVHA
Sbjct: 213 MSDLSKVDRTRTPWLLVLFHVPWYNSNKAHQGAGDDMMAVMEPLLYAASVDLVIAGHVHA 272

Query: 541 YERFNRVYNDKADACGPVHITVGDGGNREGLAKRFEDPQSPLSMFREASYGYGELYLANA 720
           YER  RVYN + D CG VHIT+GDGGNREGLA R+ +PQ   S FREAS+G+GEL + N+
Sbjct: 273 YERSKRVYNGRLDRCGAVHITIGDGGNREGLAHRYINPQPNWSEFREASFGHGELKIVNS 332

Query: 721 THALWSWRRTTDVDNSAADHVWLQNLHYSNC 813
           THA WSW R    ++  AD +W+ +L  S C
Sbjct: 333 THAFWSWHRNDYDESVKADDIWITSLVGSGC 363


>ref|XP_006352507.1| PREDICTED: purple acid phosphatase 18-like [Solanum tuberosum]
          Length = 438

 Score =  378 bits (970), Expect = e-102
 Identities = 168/271 (61%), Positives = 212/271 (78%)
 Frame = +1

Query: 1   TKWTRKTLNKVNNNPYDVLLLPGDLAYADYLQFLWDSFGRMIQPLASSRPWMVTQGNHEI 180
           T WT+ TL+ ++   YD+ LLPGDL+YAD  Q  WDSFG+++QPLASSRPWMVTQGNHE 
Sbjct: 153 TGWTKSTLDHIDQCKYDIHLLPGDLSYADCFQHRWDSFGQLVQPLASSRPWMVTQGNHEK 212

Query: 181 ENLLFIEKPFIAYNSRWKMPFAESSSPSNLFYSFEVSGAHILMLGSYANFSRDSEQFAWL 360
           E +LF+E  F++YNSRWKMPFAES S SNL+YSF+V+GAHI+MLGSYA +   S+Q++WL
Sbjct: 213 ERILFLEDGFVSYNSRWKMPFAESGSASNLYYSFDVAGAHIIMLGSYAPYDERSDQYSWL 272

Query: 361 QGDLAKMDRTRTPWLIALMHVPWYSTNKAHKGEGDDMKDAMEEILYQAKVDLVLVGHVHA 540
           + DL K+DR RTPWL+ L HVPWY++N+AH+GEGDDM  +ME +LY A  DLV  GHVHA
Sbjct: 273 KADLLKVDRKRTPWLVVLFHVPWYNSNRAHQGEGDDMMASMEPLLYAAGADLVFTGHVHA 332

Query: 541 YERFNRVYNDKADACGPVHITVGDGGNREGLAKRFEDPQSPLSMFREASYGYGELYLANA 720
           YER  RVYN K+D CG VHIT+GDGGNREGLA R++ P    S+FREAS+G+GEL + N+
Sbjct: 333 YERTKRVYNGKSDPCGAVHITIGDGGNREGLAHRYKQPPPEWSVFREASFGHGELKMVNS 392

Query: 721 THALWSWRRTTDVDNSAADHVWLQNLHYSNC 813
           THA WSW R  D ++  +D VW+ +L  S C
Sbjct: 393 THAFWSWHRNDDDESVRSDQVWITSLIGSGC 423


>ref|XP_004248333.1| PREDICTED: purple acid phosphatase 18-like [Solanum lycopersicum]
          Length = 434

 Score =  377 bits (968), Expect = e-102
 Identities = 167/271 (61%), Positives = 211/271 (77%)
 Frame = +1

Query: 1   TKWTRKTLNKVNNNPYDVLLLPGDLAYADYLQFLWDSFGRMIQPLASSRPWMVTQGNHEI 180
           T WT+ TL+ ++   YDV LLPGDL+YAD  Q+ WDSFG+++QPLASSRPWMVTQGNHE 
Sbjct: 149 TGWTKSTLDHIDQCKYDVHLLPGDLSYADCFQYRWDSFGQLVQPLASSRPWMVTQGNHEK 208

Query: 181 ENLLFIEKPFIAYNSRWKMPFAESSSPSNLFYSFEVSGAHILMLGSYANFSRDSEQFAWL 360
           E +LF+E  F++YNSRWKMPFAES S SNL+YSF+V+G HI+MLGSYA +   S+Q++WL
Sbjct: 209 ERILFLEDGFVSYNSRWKMPFAESGSTSNLYYSFDVAGVHIIMLGSYAPYDEHSDQYSWL 268

Query: 361 QGDLAKMDRTRTPWLIALMHVPWYSTNKAHKGEGDDMKDAMEEILYQAKVDLVLVGHVHA 540
           + DL K+DR +TPWL+ L HVPWY++N+AH+GEGDDM  +ME +LY A  DLV  GHVHA
Sbjct: 269 KADLLKVDRKKTPWLVVLFHVPWYNSNRAHQGEGDDMMASMEPLLYAAGADLVFTGHVHA 328

Query: 541 YERFNRVYNDKADACGPVHITVGDGGNREGLAKRFEDPQSPLSMFREASYGYGELYLANA 720
           YER  RVYN K+D CG VHIT+GDGGNREGLA R++ P    S+FREAS+G+GEL + N 
Sbjct: 329 YERTKRVYNGKSDPCGAVHITIGDGGNREGLAHRYKQPPPEWSIFREASFGHGELKMVNT 388

Query: 721 THALWSWRRTTDVDNSAADHVWLQNLHYSNC 813
           THA WSW R  D ++  +D VW+ +L  S C
Sbjct: 389 THAFWSWHRNDDDESVRSDQVWITSLIGSGC 419


>ref|XP_006851491.1| hypothetical protein AMTR_s00040p00145560 [Amborella trichopoda]
           gi|548855185|gb|ERN13072.1| hypothetical protein
           AMTR_s00040p00145560 [Amborella trichopoda]
          Length = 425

 Score =  375 bits (963), Expect = e-101
 Identities = 169/272 (62%), Positives = 212/272 (77%), Gaps = 1/272 (0%)
 Frame = +1

Query: 1   TKWTRKTLNKVNNNPYDVLLLPGDLAYADYLQFLWDSFGRMIQPLASSRPWMVTQGNHEI 180
           T WT+ TL  + N+ YD+LLLPGDL+YAD +Q LWDSFGR+I+PLAS RPWMVTQGNHEI
Sbjct: 151 TGWTKTTLQHIENSGYDMLLLPGDLSYADSIQSLWDSFGRLIEPLASQRPWMVTQGNHEI 210

Query: 181 ENLLFIEKP-FIAYNSRWKMPFAESSSPSNLFYSFEVSGAHILMLGSYANFSRDSEQFAW 357
           E +  I    F AY +RW MPF ES S SNLFYSFEV   H+LMLGSY +F + S Q+ W
Sbjct: 211 EKIFLIAPDSFKAYKARWHMPFEESGSDSNLFYSFEVPSVHVLMLGSYTDFDQGSAQYQW 270

Query: 358 LQGDLAKMDRTRTPWLIALMHVPWYSTNKAHKGEGDDMKDAMEEILYQAKVDLVLVGHVH 537
           LQ DLAK+DR RTPWL+A +H PWY+TN AH+GEG+DM+ AMEE+LY A+VD+V  GHVH
Sbjct: 271 LQSDLAKVDRKRTPWLLAFIHAPWYNTNSAHQGEGEDMRKAMEELLYGARVDVVFAGHVH 330

Query: 538 AYERFNRVYNDKADACGPVHITVGDGGNREGLAKRFEDPQSPLSMFREASYGYGELYLAN 717
           AYERF +VYN++ + CGPVHIT+GDGGN+EGLA +F DP+   S+FREAS+G+G+L +AN
Sbjct: 331 AYERFAQVYNNQPNNCGPVHITIGDGGNKEGLANKFLDPKPGWSLFREASFGHGQLQVAN 390

Query: 718 ATHALWSWRRTTDVDNSAADHVWLQNLHYSNC 813
            THALW+W R  D ++  AD +WL  L ++ C
Sbjct: 391 ETHALWTWHRNDDDESVVADRLWLTGLAHTIC 422


>ref|XP_004303000.1| PREDICTED: purple acid phosphatase 22-like [Fragaria vesca subsp.
           vesca]
          Length = 438

 Score =  374 bits (960), Expect = e-101
 Identities = 172/270 (63%), Positives = 210/270 (77%), Gaps = 1/270 (0%)
 Frame = +1

Query: 1   TKWTRKTLNKVNNNPYDVLLLPGDLAYADYLQFLWDSFGRMIQPLASSRPWMVTQGNHEI 180
           T+WT  TL+ + +  YDVLLLPGDL+YAD  Q LWDSFGR+++P AS RPWMVT+GNHEI
Sbjct: 154 TEWTNSTLDHIQSIDYDVLLLPGDLSYADTHQPLWDSFGRIVEPYASRRPWMVTEGNHEI 213

Query: 181 ENLLFIEKP-FIAYNSRWKMPFAESSSPSNLFYSFEVSGAHILMLGSYANFSRDSEQFAW 357
           E    I    F AYN+RW MP+ ES S SNL+YSFEV+G H++MLGSYA F   S+Q+ W
Sbjct: 214 EIFPIIYPTGFKAYNARWPMPYQESGSTSNLYYSFEVAGTHVVMLGSYAEFKAGSDQYQW 273

Query: 358 LQGDLAKMDRTRTPWLIALMHVPWYSTNKAHKGEGDDMKDAMEEILYQAKVDLVLVGHVH 537
           LQ DLAK+DR +TPW++AL+H PWY+TN AH+GEGD M+ AMEE+LY+A+VDLV  GHVH
Sbjct: 274 LQADLAKIDRKKTPWVVALLHAPWYNTNTAHQGEGDSMRLAMEELLYKAQVDLVFQGHVH 333

Query: 538 AYERFNRVYNDKADACGPVHITVGDGGNREGLAKRFEDPQSPLSMFREASYGYGELYLAN 717
           AYERF RVYN+KAD CGP++IT+GDGGNREGLA  FE P SPLS+FREAS+G+G L + N
Sbjct: 334 AYERFTRVYNNKADPCGPIYITIGDGGNREGLALSFEKPTSPLSLFREASFGHGRLKMVN 393

Query: 718 ATHALWSWRRTTDVDNSAADHVWLQNLHYS 807
            THALW W R  D +    D VWLQ+L  S
Sbjct: 394 ETHALWGWHRNNDSNFIVKDQVWLQSLSSS 423


>ref|XP_003617509.1| Purple acid phosphatase [Medicago truncatula]
           gi|355518844|gb|AET00468.1| Purple acid phosphatase
           [Medicago truncatula]
          Length = 439

 Score =  374 bits (960), Expect = e-101
 Identities = 166/271 (61%), Positives = 211/271 (77%)
 Frame = +1

Query: 1   TKWTRKTLNKVNNNPYDVLLLPGDLAYADYLQFLWDSFGRMIQPLASSRPWMVTQGNHEI 180
           T WT+ TL+ ++   YDV LLPGDL+YAD +Q LWDSFGR+++PLAS+RPWMVT+GNHE 
Sbjct: 154 TGWTKSTLDHIDQCKYDVYLLPGDLSYADCMQHLWDSFGRLVEPLASARPWMVTEGNHEE 213

Query: 181 ENLLFIEKPFIAYNSRWKMPFAESSSPSNLFYSFEVSGAHILMLGSYANFSRDSEQFAWL 360
           EN+  +   F++YNSRWKMPF ES S SNL+YSFEV+G H++MLGSYA++ + SEQ+ WL
Sbjct: 214 ENIPLLTDEFVSYNSRWKMPFEESGSTSNLYYSFEVAGVHVIMLGSYADYDKYSEQYRWL 273

Query: 361 QGDLAKMDRTRTPWLIALMHVPWYSTNKAHKGEGDDMKDAMEEILYQAKVDLVLVGHVHA 540
           + DL+K+DR RTPWL+ L HVPWY++NKAH+G GDDM   ME +LY A VDLVL GHVHA
Sbjct: 274 KEDLSKVDRKRTPWLVVLFHVPWYNSNKAHQGAGDDMMTVMEPLLYAASVDLVLAGHVHA 333

Query: 541 YERFNRVYNDKADACGPVHITVGDGGNREGLAKRFEDPQSPLSMFREASYGYGELYLANA 720
           YER  RVYN + D CG VHIT+GDGGNREGLA R+ +PQ   S FREAS+G+GEL + N+
Sbjct: 334 YERSKRVYNGRLDPCGAVHITIGDGGNREGLAHRYINPQPKWSEFREASFGHGELKIVNS 393

Query: 721 THALWSWRRTTDVDNSAADHVWLQNLHYSNC 813
           THA WSW R  + ++  AD +W+ +L  S C
Sbjct: 394 THAFWSWHRNDNDESIKADGIWITSLISSGC 424


>ref|XP_003625794.1| Purple acid phosphatase [Medicago truncatula]
           gi|355500809|gb|AES82012.1| Purple acid phosphatase
           [Medicago truncatula]
          Length = 444

 Score =  374 bits (960), Expect = e-101
 Identities = 169/273 (61%), Positives = 217/273 (79%), Gaps = 1/273 (0%)
 Frame = +1

Query: 1   TKWTRKTLNKVNNNPYDVLLLPGDLAYADYLQFLWDSFGRMIQPLASSRPWMVTQGNHEI 180
           T+WT  TL  V+ + YDV L+PGDL+YAD  Q LWDSFGR+++P AS RPWMVT+GNHEI
Sbjct: 159 TEWTASTLKHVDKSDYDVFLIPGDLSYADSQQPLWDSFGRLVEPYASKRPWMVTEGNHEI 218

Query: 181 ENLLFI-EKPFIAYNSRWKMPFAESSSPSNLFYSFEVSGAHILMLGSYANFSRDSEQFAW 357
           E    I  K F AYN+RW MPF ES S SNL+YSFEV+G HI+MLGSYA+FS +S+Q+ W
Sbjct: 219 EIFPIIYPKGFEAYNTRWPMPFQESGSNSNLYYSFEVAGVHIIMLGSYADFSVESQQYEW 278

Query: 358 LQGDLAKMDRTRTPWLIALMHVPWYSTNKAHKGEGDDMKDAMEEILYQAKVDLVLVGHVH 537
           LQ DL K+DR +TPW+I ++H PWY+TN+AH+GEG+ M+ AMEE+L++A+VDLV  GHVH
Sbjct: 279 LQLDLTKIDRVKTPWVITMVHAPWYTTNEAHQGEGESMRQAMEELLFKARVDLVFAGHVH 338

Query: 538 AYERFNRVYNDKADACGPVHITVGDGGNREGLAKRFEDPQSPLSMFREASYGYGELYLAN 717
           AYERF R+YN+KAD+CGP+++T+GDGGNREGLA RF++P SPLS+FRE S+G+G L + N
Sbjct: 339 AYERFTRIYNNKADSCGPMYVTIGDGGNREGLALRFKNPPSPLSLFREPSFGHGRLRILN 398

Query: 718 ATHALWSWRRTTDVDNSAADHVWLQNLHYSNCK 816
            THA WSW R  D D   AD +W+++L  SN K
Sbjct: 399 ETHAHWSWHRNNDKDAIVADGIWIESL--SNLK 429


>ref|XP_006588840.1| PREDICTED: purple acid phosphatase 22-like [Glycine max]
          Length = 437

 Score =  374 bits (959), Expect = e-101
 Identities = 165/270 (61%), Positives = 212/270 (78%), Gaps = 1/270 (0%)
 Frame = +1

Query: 1   TKWTRKTLNKVNNNPYDVLLLPGDLAYADYLQFLWDSFGRMIQPLASSRPWMVTQGNHEI 180
           T+WT  TL  V++  YDV LLPGDL+YAD  Q LWDSFGR+++P AS  PWMVT+GNHEI
Sbjct: 152 TEWTTSTLKHVDSKDYDVFLLPGDLSYADTHQPLWDSFGRLVEPYASRIPWMVTEGNHEI 211

Query: 181 ENLLFIE-KPFIAYNSRWKMPFAESSSPSNLFYSFEVSGAHILMLGSYANFSRDSEQFAW 357
           E    I+   F AYN+RW MP+ ES S SNL+YSF+V+  H++MLGSY +F   S+Q+ W
Sbjct: 212 ETFPIIQPNGFKAYNARWPMPYKESGSTSNLYYSFDVASTHVIMLGSYTDFDAHSQQYTW 271

Query: 358 LQGDLAKMDRTRTPWLIALMHVPWYSTNKAHKGEGDDMKDAMEEILYQAKVDLVLVGHVH 537
           LQ DLAK+DR RTPW+IAL+H PWY+TN+AH+GEG+DM+ AMEE+LY+A+VDLV  GHVH
Sbjct: 272 LQSDLAKIDRKRTPWVIALLHAPWYNTNEAHQGEGEDMRQAMEELLYEARVDLVFAGHVH 331

Query: 538 AYERFNRVYNDKADACGPVHITVGDGGNREGLAKRFEDPQSPLSMFREASYGYGELYLAN 717
           AYERF R+Y++KAD+CGP+++T+GDGGNREGLA RF+ P SPLS++RE S+G+G L + N
Sbjct: 332 AYERFTRIYDNKADSCGPLYVTIGDGGNREGLALRFKKPPSPLSLYREPSFGHGRLRIVN 391

Query: 718 ATHALWSWRRTTDVDNSAADHVWLQNLHYS 807
            THA WSW R  D D   AD VW+++L  S
Sbjct: 392 ETHAYWSWHRNNDTDTFVADGVWIESLSNS 421


>ref|XP_002278488.2| PREDICTED: purple acid phosphatase 18-like [Vitis vinifera]
           gi|297737421|emb|CBI26622.3| unnamed protein product
           [Vitis vinifera]
          Length = 432

 Score =  373 bits (957), Expect = e-101
 Identities = 164/271 (60%), Positives = 211/271 (77%)
 Frame = +1

Query: 1   TKWTRKTLNKVNNNPYDVLLLPGDLAYADYLQFLWDSFGRMIQPLASSRPWMVTQGNHEI 180
           T WT+ TL+ ++   YDV LLPGDL+YADYLQ  WD+FG ++QPLAS+RPWMVT+GNHE 
Sbjct: 147 TGWTKSTLDHIDGCNYDVHLLPGDLSYADYLQRRWDTFGELVQPLASARPWMVTEGNHEQ 206

Query: 181 ENLLFIEKPFIAYNSRWKMPFAESSSPSNLFYSFEVSGAHILMLGSYANFSRDSEQFAWL 360
           EN+ F +  F +YNSRW MP+ ES SPSNL+YSFEV+G H++MLGSYA +  +S Q++WL
Sbjct: 207 ENIPFFKDGFESYNSRWTMPYQESGSPSNLYYSFEVAGVHVVMLGSYAAYDLNSNQYSWL 266

Query: 361 QGDLAKMDRTRTPWLIALMHVPWYSTNKAHKGEGDDMKDAMEEILYQAKVDLVLVGHVHA 540
           + DL+++DR RTPWL+ L+HVPWY++NKAH+GEGD M + +E +LY A VDLV  GHVHA
Sbjct: 267 KTDLSRVDRKRTPWLLVLLHVPWYNSNKAHQGEGDRMMETLEPLLYAANVDLVFAGHVHA 326

Query: 541 YERFNRVYNDKADACGPVHITVGDGGNREGLAKRFEDPQSPLSMFREASYGYGELYLANA 720
           YER  RVYN ++D CGP+HIT+GDGGNREGLA R+ DPQ   S+FREAS+G+GEL + N 
Sbjct: 327 YERSKRVYNGRSDPCGPIHITIGDGGNREGLATRYNDPQPEWSVFREASFGHGELKIVNL 386

Query: 721 THALWSWRRTTDVDNSAADHVWLQNLHYSNC 813
           THA WSW R  D +   +D VW+ +L  S C
Sbjct: 387 THAFWSWHRNDDDEPVRSDEVWITSLVGSGC 417


>ref|XP_006858168.1| hypothetical protein AMTR_s00062p00146590 [Amborella trichopoda]
           gi|548862271|gb|ERN19635.1| hypothetical protein
           AMTR_s00062p00146590 [Amborella trichopoda]
          Length = 436

 Score =  372 bits (955), Expect = e-101
 Identities = 165/271 (60%), Positives = 215/271 (79%)
 Frame = +1

Query: 1   TKWTRKTLNKVNNNPYDVLLLPGDLAYADYLQFLWDSFGRMIQPLASSRPWMVTQGNHEI 180
           T WT  TL+ ++   YDV LLPGDL+YADY Q LWDSFG ++QPLAS+RPWMVT+GNHE 
Sbjct: 151 TGWTSSTLDHIDKCEYDVHLLPGDLSYADYRQHLWDSFGELVQPLASARPWMVTEGNHEK 210

Query: 181 ENLLFIEKPFIAYNSRWKMPFAESSSPSNLFYSFEVSGAHILMLGSYANFSRDSEQFAWL 360
           E++ F +  F AYN+RWKMPF ES S SNL+YSFEV+G H+LMLGSY ++ + S+Q+ WL
Sbjct: 211 ESIPFFKAGFDAYNARWKMPFEESGSGSNLYYSFEVAGVHLLMLGSYTDYDKYSDQYKWL 270

Query: 361 QGDLAKMDRTRTPWLIALMHVPWYSTNKAHKGEGDDMKDAMEEILYQAKVDLVLVGHVHA 540
           + DLAK+DR RTPWLIAL HVPWY++N AH+GEGDDM  A+E +LY+AKVD++L GHVHA
Sbjct: 271 KADLAKVDRKRTPWLIALFHVPWYNSNWAHQGEGDDMMAAVEPLLYEAKVDIMLAGHVHA 330

Query: 541 YERFNRVYNDKADACGPVHITVGDGGNREGLAKRFEDPQSPLSMFREASYGYGELYLANA 720
           YER NRV   K D+CG +HIT+GDGGNREGLA+R+++P+   SMFREAS+G+GEL + N+
Sbjct: 331 YERSNRVNKGKVDSCGAIHITIGDGGNREGLAQRYKNPKPEWSMFREASFGHGELQVVNS 390

Query: 721 THALWSWRRTTDVDNSAADHVWLQNLHYSNC 813
           +HA WSW R  + ++  +D +W+ +L  S C
Sbjct: 391 SHAFWSWHRNDNDESVRSDQLWIHSLTSSGC 421


>ref|XP_004517048.1| PREDICTED: purple acid phosphatase 18-like isoform X1 [Cicer
           arietinum]
          Length = 437

 Score =  372 bits (955), Expect = e-101
 Identities = 165/271 (60%), Positives = 210/271 (77%)
 Frame = +1

Query: 1   TKWTRKTLNKVNNNPYDVLLLPGDLAYADYLQFLWDSFGRMIQPLASSRPWMVTQGNHEI 180
           T WT+ TL+ ++   YDV LLPGDL+YAD +Q LWDSFGR+++PLAS+RPWMVT+GNHE 
Sbjct: 152 TGWTKSTLDHIDQCKYDVYLLPGDLSYADCMQHLWDSFGRLVEPLASARPWMVTEGNHEE 211

Query: 181 ENLLFIEKPFIAYNSRWKMPFAESSSPSNLFYSFEVSGAHILMLGSYANFSRDSEQFAWL 360
           EN+LF+   F++YNSRWKMPF ES S SNL+YSFEV+G H++MLGSYA++   SEQ+ WL
Sbjct: 212 ENILFLTDEFVSYNSRWKMPFEESGSTSNLYYSFEVAGVHVIMLGSYADYDESSEQYTWL 271

Query: 361 QGDLAKMDRTRTPWLIALMHVPWYSTNKAHKGEGDDMKDAMEEILYQAKVDLVLVGHVHA 540
             DL+K+DRTRTPWL+ L HVPWY++N+A +G GDDM   ME +LY A VDLV+ G+VHA
Sbjct: 272 MADLSKVDRTRTPWLLVLFHVPWYNSNRAQQGAGDDMMAVMEPLLYAASVDLVIAGNVHA 331

Query: 541 YERFNRVYNDKADACGPVHITVGDGGNREGLAKRFEDPQSPLSMFREASYGYGELYLANA 720
           YER  RVYN + D CG VHIT+GDGGNREGLA R+ +PQ   S FREAS+G+GEL + N+
Sbjct: 332 YERSKRVYNGRLDRCGAVHITIGDGGNREGLAHRYINPQPNWSEFREASFGHGELKIVNS 391

Query: 721 THALWSWRRTTDVDNSAADHVWLQNLHYSNC 813
           THA WSW R    ++  AD +W+ +L  S C
Sbjct: 392 THAFWSWHRNDYDESVKADDIWITSLVGSGC 422


>ref|XP_004500050.1| PREDICTED: purple acid phosphatase 18-like [Cicer arietinum]
          Length = 428

 Score =  372 bits (955), Expect = e-101
 Identities = 166/271 (61%), Positives = 209/271 (77%)
 Frame = +1

Query: 1   TKWTRKTLNKVNNNPYDVLLLPGDLAYADYLQFLWDSFGRMIQPLASSRPWMVTQGNHEI 180
           T WT+ TL+ ++   YDV L+PGDL+YADY+Q  WDSFGR++QPLAS+RPWMVTQGNHE+
Sbjct: 149 TGWTKSTLDHIDRCKYDVNLIPGDLSYADYIQHRWDSFGRLVQPLASARPWMVTQGNHEV 208

Query: 181 ENLLFIEKPFIAYNSRWKMPFAESSSPSNLFYSFEVSGAHILMLGSYANFSRDSEQFAWL 360
           E++  I+  FI+YNSRWKMPF ES S SNL+YSFEV+GAHI+MLGSYA++   SEQ+ WL
Sbjct: 209 EHIPLIKDGFISYNSRWKMPFEESGSSSNLYYSFEVAGAHIIMLGSYADYDEYSEQYTWL 268

Query: 361 QGDLAKMDRTRTPWLIALMHVPWYSTNKAHKGEGDDMKDAMEEILYQAKVDLVLVGHVHA 540
           + DL+K+DR RTPWL+ L HVPWY++N AH+GEG DM   ME +LY A VDLV  GHVHA
Sbjct: 269 KTDLSKVDRKRTPWLLVLFHVPWYNSNTAHQGEGGDMMQTMEPLLYAANVDLVFAGHVHA 328

Query: 541 YERFNRVYNDKADACGPVHITVGDGGNREGLAKRFEDPQSPLSMFREASYGYGELYLANA 720
           YER  RVYN + D CG VHIT+GDGGN+EGLA ++  PQ   S FREAS+G+GEL + N+
Sbjct: 329 YERSKRVYNGRLDPCGAVHITIGDGGNKEGLAHKYITPQPKWSDFREASFGHGELKIVNS 388

Query: 721 THALWSWRRTTDVDNSAADHVWLQNLHYSNC 813
           THA WSW R  D +   +D +W+ +L  S C
Sbjct: 389 THAFWSWHRNDDDEPVKSDDIWINSLVSSGC 419


>ref|NP_001150058.1| LOC100283685 precursor [Zea mays] gi|195636356|gb|ACG37646.1|
           purple acid phosphatase precursor [Zea mays]
           gi|414871534|tpg|DAA50091.1| TPA: purple acid
           phosphatase [Zea mays]
          Length = 460

 Score =  372 bits (954), Expect = e-100
 Identities = 164/271 (60%), Positives = 211/271 (77%)
 Frame = +1

Query: 1   TKWTRKTLNKVNNNPYDVLLLPGDLAYADYLQFLWDSFGRMIQPLASSRPWMVTQGNHEI 180
           T WT  TLN +    +D+LLLPGDL+YADY+Q LWDSFG +++PLAS+RPWMVT+GNHE 
Sbjct: 173 TSWTTSTLNHIKQCEHDMLLLPGDLSYADYMQHLWDSFGTLVEPLASNRPWMVTEGNHEK 232

Query: 181 ENLLFIEKPFIAYNSRWKMPFAESSSPSNLFYSFEVSGAHILMLGSYANFSRDSEQFAWL 360
           E++ F E  F +YN+RWKMP+ ES S SNL+YSFEV+GAHI+MLGSY ++   S+Q+AWL
Sbjct: 233 EHIPFFESGFQSYNARWKMPYEESGSRSNLYYSFEVAGAHIIMLGSYTDYDDSSDQYAWL 292

Query: 361 QGDLAKMDRTRTPWLIALMHVPWYSTNKAHKGEGDDMKDAMEEILYQAKVDLVLVGHVHA 540
           + DLAK+DR RTPWLI L+HVPWY++N AH+GEGD M  +ME +LY A VD+V+ GHVHA
Sbjct: 293 KADLAKVDRKRTPWLIVLLHVPWYNSNWAHQGEGDSMMASMEPLLYAAHVDMVIAGHVHA 352

Query: 541 YERFNRVYNDKADACGPVHITVGDGGNREGLAKRFEDPQSPLSMFREASYGYGELYLANA 720
           YER  RVYN + D CG VHIT+GDGGNREGLA+R+ +P+   S+FREAS+G+GEL + N+
Sbjct: 353 YERAERVYNSRPDPCGAVHITIGDGGNREGLARRYRNPKPAWSVFREASFGHGELKIVNS 412

Query: 721 THALWSWRRTTDVDNSAADHVWLQNLHYSNC 813
           THA W+W R  D +    D VW+ +L  S C
Sbjct: 413 THAHWTWHRNDDEEPVRTDDVWINSLAGSGC 443


>ref|XP_003600057.1| Purple acid phosphatase [Medicago truncatula]
           gi|355489105|gb|AES70308.1| Purple acid phosphatase
           [Medicago truncatula]
          Length = 433

 Score =  371 bits (953), Expect = e-100
 Identities = 165/271 (60%), Positives = 210/271 (77%)
 Frame = +1

Query: 1   TKWTRKTLNKVNNNPYDVLLLPGDLAYADYLQFLWDSFGRMIQPLASSRPWMVTQGNHEI 180
           T WT+ TL+ ++   YDV L+PGDL+YADY+Q  WD+FGR++QPLASSRPWMVTQGNHE+
Sbjct: 148 TGWTKSTLDHIDQCKYDVNLIPGDLSYADYIQHRWDTFGRLVQPLASSRPWMVTQGNHEV 207

Query: 181 ENLLFIEKPFIAYNSRWKMPFAESSSPSNLFYSFEVSGAHILMLGSYANFSRDSEQFAWL 360
           E++  ++  FI+YNSRWKMPF ES S SNL+YSFEV+GAHI+MLGSY ++   SEQ+ WL
Sbjct: 208 EHIPLLKDGFISYNSRWKMPFEESGSSSNLYYSFEVAGAHIIMLGSYDDYDVYSEQYKWL 267

Query: 361 QGDLAKMDRTRTPWLIALMHVPWYSTNKAHKGEGDDMKDAMEEILYQAKVDLVLVGHVHA 540
           + DL+K+DR RTPWL+ + HVPWY++N AH+GEG DM + ME +LY A VDLV  GHVHA
Sbjct: 268 KTDLSKVDRKRTPWLLVIFHVPWYNSNTAHQGEGGDMMETMEPLLYAASVDLVFAGHVHA 327

Query: 541 YERFNRVYNDKADACGPVHITVGDGGNREGLAKRFEDPQSPLSMFREASYGYGELYLANA 720
           YER  RVYN K D CG VHIT+GDGGN+EGLA ++ DPQ   S FREAS+G+GEL + N+
Sbjct: 328 YERSKRVYNGKLDPCGAVHITIGDGGNKEGLAHKYIDPQPKWSEFREASFGHGELKIVNS 387

Query: 721 THALWSWRRTTDVDNSAADHVWLQNLHYSNC 813
           THA WSW R  D +   +D +W+ +L  S C
Sbjct: 388 THAFWSWHRNDDDEPVKSDDIWITSLVNSGC 418


>ref|XP_002317591.1| calcineurin-like phosphoesterase family protein [Populus
           trichocarpa] gi|222860656|gb|EEE98203.1|
           calcineurin-like phosphoesterase family protein [Populus
           trichocarpa]
          Length = 432

 Score =  371 bits (952), Expect = e-100
 Identities = 167/271 (61%), Positives = 210/271 (77%)
 Frame = +1

Query: 1   TKWTRKTLNKVNNNPYDVLLLPGDLAYADYLQFLWDSFGRMIQPLASSRPWMVTQGNHEI 180
           T WT+ TL+ ++   YDV LLPGDL+YADY+Q LWD+FG +++PLAS+RPWMVTQGNHE 
Sbjct: 147 TGWTQSTLDHIDQCKYDVHLLPGDLSYADYMQHLWDTFGELVEPLASARPWMVTQGNHER 206

Query: 181 ENLLFIEKPFIAYNSRWKMPFAESSSPSNLFYSFEVSGAHILMLGSYANFSRDSEQFAWL 360
           E++ F++  F  YNSRWKMPF ES S SNL+YSFEVSGAHI+MLGSY  +   S Q+ WL
Sbjct: 207 ESIPFLKDGFEPYNSRWKMPFEESGSSSNLYYSFEVSGAHIIMLGSYTGYDEYSNQYNWL 266

Query: 361 QGDLAKMDRTRTPWLIALMHVPWYSTNKAHKGEGDDMKDAMEEILYQAKVDLVLVGHVHA 540
           + DLAK+DR +TPWL+ L HVPWY++N+AH+ EGD M +AME +LY A VD+VL GHVHA
Sbjct: 267 EADLAKVDRNKTPWLLVLFHVPWYNSNEAHQNEGDRMMEAMEPLLYAASVDIVLAGHVHA 326

Query: 541 YERFNRVYNDKADACGPVHITVGDGGNREGLAKRFEDPQSPLSMFREASYGYGELYLANA 720
           YER  RV N K D CG VHIT+GDGGNREGLA ++++PQ   S+FREAS+G+GEL LAN+
Sbjct: 327 YERTERVNNGKLDPCGAVHITIGDGGNREGLAHKYKNPQPAWSVFREASFGHGELKLANS 386

Query: 721 THALWSWRRTTDVDNSAADHVWLQNLHYSNC 813
           THA WSW R  D +   +D VW+ +L  S C
Sbjct: 387 THAFWSWHRNDDDEPVRSDQVWITSLVNSEC 417


>gb|ADM32502.1| purple acid phosphatases [Glycine max]
          Length = 437

 Score =  370 bits (951), Expect = e-100
 Identities = 164/271 (60%), Positives = 210/271 (77%)
 Frame = +1

Query: 1   TKWTRKTLNKVNNNPYDVLLLPGDLAYADYLQFLWDSFGRMIQPLASSRPWMVTQGNHEI 180
           T WT+ TL  ++   YDV LLPGDL+YAD +Q LWD+FG++++PLAS+RPWMVT+GNHE 
Sbjct: 152 TGWTKSTLAHIDQCKYDVYLLPGDLSYADCMQHLWDNFGKLVEPLASTRPWMVTEGNHEE 211

Query: 181 ENLLFIEKPFIAYNSRWKMPFAESSSPSNLFYSFEVSGAHILMLGSYANFSRDSEQFAWL 360
           EN+L +   F++YNSRWKMP+ ES S SNL+YSFEV+G H++MLGSYA++   SEQ+ WL
Sbjct: 212 ENILLLTDEFVSYNSRWKMPYEESGSTSNLYYSFEVAGVHVIMLGSYADYDVYSEQYRWL 271

Query: 361 QGDLAKMDRTRTPWLIALMHVPWYSTNKAHKGEGDDMKDAMEEILYQAKVDLVLVGHVHA 540
           + DL+K+DR RTPWL+ L HVPWY++NKAH+G GDDM  AME +LY A VDLV+ GHVHA
Sbjct: 272 KEDLSKVDRKRTPWLLVLFHVPWYNSNKAHQGAGDDMMAAMEPLLYAASVDLVIAGHVHA 331

Query: 541 YERFNRVYNDKADACGPVHITVGDGGNREGLAKRFEDPQSPLSMFREASYGYGELYLANA 720
           YER  RVYN + D CG VHIT+GDGGNREGLA ++ +PQ   S FREAS+G+GEL + N+
Sbjct: 332 YERSKRVYNGRLDPCGAVHITIGDGGNREGLAHKYINPQPKWSEFREASFGHGELKIVNS 391

Query: 721 THALWSWRRTTDVDNSAADHVWLQNLHYSNC 813
           THA WSW R  D +   AD +W+ +L  S C
Sbjct: 392 THAFWSWHRNDDDEPVKADDIWITSLASSGC 422


>gb|ACG42188.1| purple acid phosphatase precursor [Zea mays]
          Length = 457

 Score =  370 bits (950), Expect = e-100
 Identities = 163/271 (60%), Positives = 210/271 (77%)
 Frame = +1

Query: 1   TKWTRKTLNKVNNNPYDVLLLPGDLAYADYLQFLWDSFGRMIQPLASSRPWMVTQGNHEI 180
           T WT  TLN +    +D+LLLPGDL+YADY+Q LWDSFG +++PLAS+RPWMVT+GNHE 
Sbjct: 170 TSWTTSTLNHIKQCEHDMLLLPGDLSYADYMQHLWDSFGTLVEPLASNRPWMVTEGNHEK 229

Query: 181 ENLLFIEKPFIAYNSRWKMPFAESSSPSNLFYSFEVSGAHILMLGSYANFSRDSEQFAWL 360
           E++ F E  F +YN+RWKMP+ ES S SNL+YSFEV+GAHI+MLGSY ++   S+Q+AWL
Sbjct: 230 EHIPFFESGFQSYNARWKMPYEESGSRSNLYYSFEVAGAHIIMLGSYTDYDDSSDQYAWL 289

Query: 361 QGDLAKMDRTRTPWLIALMHVPWYSTNKAHKGEGDDMKDAMEEILYQAKVDLVLVGHVHA 540
           + DL K+DR RTPWLI L+HVPWY++N AH+GEGD M  +ME +LY A VD+V+ GHVHA
Sbjct: 290 KADLVKVDRKRTPWLIVLLHVPWYNSNWAHQGEGDSMMASMEPLLYAAHVDMVIAGHVHA 349

Query: 541 YERFNRVYNDKADACGPVHITVGDGGNREGLAKRFEDPQSPLSMFREASYGYGELYLANA 720
           YER  RVYN + D CG VHIT+GDGGNREGLA+R+ +P+   S+FREAS+G+GEL + N+
Sbjct: 350 YERAERVYNSRPDPCGAVHITIGDGGNREGLARRYRNPKPAWSVFREASFGHGELKIVNS 409

Query: 721 THALWSWRRTTDVDNSAADHVWLQNLHYSNC 813
           THA W+W R  D +    D VW+ +L  S C
Sbjct: 410 THAHWTWHRNDDEEPVRTDDVWINSLAGSGC 440


>ref|XP_003521454.2| PREDICTED: purple acid phosphatase 22 [Glycine max]
          Length = 491

 Score =  370 bits (949), Expect = e-100
 Identities = 163/270 (60%), Positives = 211/270 (78%), Gaps = 1/270 (0%)
 Frame = +1

Query: 1    TKWTRKTLNKVNNNPYDVLLLPGDLAYADYLQFLWDSFGRMIQPLASSRPWMVTQGNHEI 180
            T+WT  TL  V++N YDV LLPGDL+YAD  Q LWDSFGR+++P AS RPWMVT+GNHEI
Sbjct: 205  TEWTASTLKHVDSNDYDVFLLPGDLSYADSQQPLWDSFGRLVEPYASKRPWMVTEGNHEI 264

Query: 181  ENLLFI-EKPFIAYNSRWKMPFAESSSPSNLFYSFEVSGAHILMLGSYANFSRDSEQFAW 357
            E+   I  + F AYN+RW MPF +S S SNL+YSFEV+  H +MLGSY +F   S+Q+ W
Sbjct: 265  ESFPIIYPQGFQAYNARWPMPFQQSGSTSNLYYSFEVTATHFIMLGSYTDFDAQSQQYTW 324

Query: 358  LQGDLAKMDRTRTPWLIALMHVPWYSTNKAHKGEGDDMKDAMEEILYQAKVDLVLVGHVH 537
            LQ DLA +DR +TPW+I L+H PWY+TN+AH+GEG+ M+ AMEE+LY+A+VDLV  GHVH
Sbjct: 325  LQSDLANIDRAKTPWVIVLLHAPWYNTNEAHQGEGESMRQAMEELLYEARVDLVFAGHVH 384

Query: 538  AYERFNRVYNDKADACGPVHITVGDGGNREGLAKRFEDPQSPLSMFREASYGYGELYLAN 717
            AYERF R+Y++KAD+CGP+++T+GDGGNREGLA  F++P SPLS++RE S+G+G L + N
Sbjct: 385  AYERFTRIYDNKADSCGPMYVTIGDGGNREGLALMFKNPPSPLSLYREPSFGHGRLRILN 444

Query: 718  ATHALWSWRRTTDVDNSAADHVWLQNLHYS 807
             THA WSW R  D D   AD VW+++L  S
Sbjct: 445  ETHAHWSWHRNNDADAVVADGVWIESLSSS 474


>ref|XP_004982542.1| PREDICTED: purple acid phosphatase 18-like [Setaria italica]
          Length = 472

 Score =  370 bits (949), Expect = e-100
 Identities = 163/271 (60%), Positives = 210/271 (77%)
 Frame = +1

Query: 1   TKWTRKTLNKVNNNPYDVLLLPGDLAYADYLQFLWDSFGRMIQPLASSRPWMVTQGNHEI 180
           T WT  TLN +    +D+LLLPGDL+YAD++Q LWDSFG +++PLAS+RPWMVT+GNHE 
Sbjct: 187 TSWTTSTLNHIKQCEHDMLLLPGDLSYADFMQHLWDSFGTLVEPLASTRPWMVTEGNHEK 246

Query: 181 ENLLFIEKPFIAYNSRWKMPFAESSSPSNLFYSFEVSGAHILMLGSYANFSRDSEQFAWL 360
           E++ F+E  F +YN+RWKMP+ ES S SNL+YSFEV+GAHI+MLGSY ++   S+Q+AWL
Sbjct: 247 EHIPFLESGFQSYNARWKMPYEESGSTSNLYYSFEVAGAHIIMLGSYTDYDETSDQYAWL 306

Query: 361 QGDLAKMDRTRTPWLIALMHVPWYSTNKAHKGEGDDMKDAMEEILYQAKVDLVLVGHVHA 540
           + DLAK+DR RTPWLI L+H PWY++N AH+GEGD M  AME +LY A VD+V+ GHVHA
Sbjct: 307 KADLAKVDRKRTPWLIVLLHAPWYNSNWAHQGEGDSMMAAMEPLLYAAHVDMVVAGHVHA 366

Query: 541 YERFNRVYNDKADACGPVHITVGDGGNREGLAKRFEDPQSPLSMFREASYGYGELYLANA 720
           YER  RVYN + D CG VHIT+GDGGNREGLA R+ +P+   S+FREAS+G+GEL + N+
Sbjct: 367 YERAERVYNGRLDPCGAVHITIGDGGNREGLAHRYRNPKPTWSVFREASFGHGELKIVNS 426

Query: 721 THALWSWRRTTDVDNSAADHVWLQNLHYSNC 813
           THA W+W R  D +    D VW+ +L  S C
Sbjct: 427 THAHWTWHRNDDEEPVRTDEVWIYSLSGSGC 457