BLASTX nr result
ID: Ephedra26_contig00012987
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Ephedra26_contig00012987
(521 letters)
Database: ./nr
37,332,560 sequences; 13,225,080,153 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|ABK24764.1| unknown [Picea sitchensis] 141 1e-31
gb|ABK24579.1| unknown [Picea sitchensis] 140 2e-31
ref|XP_006838224.1| hypothetical protein AMTR_s00217p00020070 [A... 134 1e-29
ref|XP_004986959.1| PREDICTED: probable polyamine transporter At... 131 8e-29
ref|XP_002283522.1| PREDICTED: uncharacterized transporter lpg16... 131 8e-29
ref|XP_004294992.1| PREDICTED: probable polyamine transporter At... 125 4e-27
ref|XP_003561594.1| PREDICTED: uncharacterized transporter lpg16... 125 4e-27
gb|EXB75232.1| Arginine/agmatine antiporter [Morus notabilis] 125 6e-27
ref|XP_006838223.1| hypothetical protein AMTR_s00217p00018260 [A... 125 8e-27
ref|XP_002465208.1| hypothetical protein SORBIDRAFT_01g034150 [S... 125 8e-27
dbj|BAJ96201.1| predicted protein [Hordeum vulgare subsp. vulgare] 124 1e-26
dbj|BAJ85124.1| predicted protein [Hordeum vulgare subsp. vulgare] 124 1e-26
gb|EMS64845.1| hypothetical protein TRIUR3_17687 [Triticum urartu] 124 2e-26
ref|XP_006492719.1| PREDICTED: probable polyamine transporter At... 123 2e-26
gb|EMT21767.1| hypothetical protein F775_02768 [Aegilops tauschii] 123 2e-26
gb|EMJ06341.1| hypothetical protein PRUPE_ppa005207mg [Prunus pe... 123 2e-26
gb|EOY32321.1| Amino acid permease family protein isoform 1 [The... 121 8e-26
gb|EMT03109.1| hypothetical protein F775_15650 [Aegilops tauschii] 121 8e-26
ref|XP_004228661.1| PREDICTED: probable polyamine transporter At... 121 8e-26
ref|XP_006445660.1| hypothetical protein CICLE_v10015109mg [Citr... 121 1e-25
>gb|ABK24764.1| unknown [Picea sitchensis]
Length = 487
Score = 141 bits (355), Expect = 1e-31
Identities = 71/113 (62%), Positives = 85/113 (75%)
Frame = +1
Query: 1 GILMSAGGTLLLSFVSFTNIVAAANFLYSCGMLLEFAAFLWLRRKRPDLKRPFRVPMEMR 180
GI SA GTL+LS+VSF NIV+AANFLYSCGMLLEFA+FLWLRRK P LKRP+RVP+ +
Sbjct: 347 GIAASACGTLVLSYVSFANIVSAANFLYSCGMLLEFASFLWLRRKFPSLKRPYRVPLGIP 406
Query: 181 GLVVMCGVPVCFLVAVMVMGNWVVYVLSVGLTALGVGAYFVMQWMKKNESMRF 339
GLV MC VPV FL+ VM + N VVY+L +T +GV YF+M KK + F
Sbjct: 407 GLVCMCAVPVVFLIFVMTLANSVVYILGSSVTVVGVLGYFLMIACKKRNWIAF 459
>gb|ABK24579.1| unknown [Picea sitchensis]
Length = 487
Score = 140 bits (352), Expect = 2e-31
Identities = 70/113 (61%), Positives = 85/113 (75%)
Frame = +1
Query: 1 GILMSAGGTLLLSFVSFTNIVAAANFLYSCGMLLEFAAFLWLRRKRPDLKRPFRVPMEMR 180
GI SA GTL+LS+VSF NIV+AANFLYSCGMLLEFA+FLWLRRK P LKRP+RVP+ +
Sbjct: 347 GIAASACGTLVLSYVSFANIVSAANFLYSCGMLLEFASFLWLRRKFPSLKRPYRVPLGIP 406
Query: 181 GLVVMCGVPVCFLVAVMVMGNWVVYVLSVGLTALGVGAYFVMQWMKKNESMRF 339
GLV MC VPV FL+ VM + N VVY+L +T +GV YF+M K+ + F
Sbjct: 407 GLVCMCAVPVVFLIFVMTLANSVVYILGSSVTVVGVLGYFLMIACKRRNWIAF 459
>ref|XP_006838224.1| hypothetical protein AMTR_s00217p00020070 [Amborella trichopoda]
gi|548840691|gb|ERN00793.1| hypothetical protein
AMTR_s00217p00020070 [Amborella trichopoda]
Length = 466
Score = 134 bits (337), Expect = 1e-29
Identities = 62/113 (54%), Positives = 88/113 (77%)
Frame = +1
Query: 1 GILMSAGGTLLLSFVSFTNIVAAANFLYSCGMLLEFAAFLWLRRKRPDLKRPFRVPMEMR 180
G+++SA G+L +SF+SFT+I+++ANFLYSCGMLLEFA+FLWLR K+ ++ RP+RVPM M
Sbjct: 344 GVVVSAAGSLAISFMSFTDIISSANFLYSCGMLLEFASFLWLRWKKGEVMRPYRVPMGMA 403
Query: 181 GLVVMCGVPVCFLVAVMVMGNWVVYVLSVGLTALGVGAYFVMQWMKKNESMRF 339
GLV +C VP FLV VM M +V +S G+T +GV AYF+M W ++++ + F
Sbjct: 404 GLVGLCLVPSAFLVFVMTMATKMVCAISAGVTVVGVAAYFLMGWCRQSKWLEF 456
>ref|XP_004986959.1| PREDICTED: probable polyamine transporter At3g13620-like [Setaria
italica]
Length = 491
Score = 131 bits (330), Expect = 8e-29
Identities = 64/112 (57%), Positives = 80/112 (71%)
Frame = +1
Query: 4 ILMSAGGTLLLSFVSFTNIVAAANFLYSCGMLLEFAAFLWLRRKRPDLKRPFRVPMEMRG 183
I+ ++ TL +SF SF NIVAAANFLYS GMLLEFAAF+WLR KRPDL RP+RVP + G
Sbjct: 357 IVTTSAITLGMSFFSFNNIVAAANFLYSLGMLLEFAAFIWLRIKRPDLSRPYRVPARLPG 416
Query: 184 LVVMCGVPVCFLVAVMVMGNWVVYVLSVGLTALGVGAYFVMQWMKKNESMRF 339
V +C VP FLV VM + W VY +S TA GVG Y++M++ K+ +RF
Sbjct: 417 AVALCLVPSAFLVFVMAIAGWKVYAISAAFTAAGVGVYYLMRFCKERGCLRF 468
>ref|XP_002283522.1| PREDICTED: uncharacterized transporter lpg1691-like [Vitis
vinifera]
Length = 473
Score = 131 bits (330), Expect = 8e-29
Identities = 59/123 (47%), Positives = 92/123 (74%)
Frame = +1
Query: 1 GILMSAGGTLLLSFVSFTNIVAAANFLYSCGMLLEFAAFLWLRRKRPDLKRPFRVPMEMR 180
GIL+S T+ +S++ F+NIV++ANFLYS GMLLEFA++LWLRRK+P LKRP+RVPM +
Sbjct: 344 GILLSTAITIGVSYMDFSNIVSSANFLYSLGMLLEFASYLWLRRKQPTLKRPYRVPMRLP 403
Query: 181 GLVVMCGVPVCFLVAVMVMGNWVVYVLSVGLTALGVGAYFVMQWMKKNESMRFVRDVACE 360
GL++MC +P FL+ +M + +VY++S +T G+G Y++M++ KK + +F +V
Sbjct: 404 GLIIMCLIPSGFLIVIMAIATKIVYLISGLVTVFGIGWYYLMKFCKKKKWFKFSHEVQQY 463
Query: 361 DHD 369
+H+
Sbjct: 464 EHE 466
>ref|XP_004294992.1| PREDICTED: probable polyamine transporter At3g13620-like [Fragaria
vesca subsp. vesca]
Length = 468
Score = 125 bits (315), Expect = 4e-27
Identities = 60/116 (51%), Positives = 87/116 (75%)
Frame = +1
Query: 1 GILMSAGGTLLLSFVSFTNIVAAANFLYSCGMLLEFAAFLWLRRKRPDLKRPFRVPMEMR 180
GIL+S TL +S+++FT+I+++ANFLYS GMLLEFA+F+WLRRK P LKRPFRVPM++
Sbjct: 345 GILISTMITLGVSYMNFTDIISSANFLYSLGMLLEFASFIWLRRKMPTLKRPFRVPMKLP 404
Query: 181 GLVVMCGVPVCFLVAVMVMGNWVVYVLSVGLTALGVGAYFVMQWMKKNESMRFVRD 348
GLV+MC +P FL+ VM + VY++S ++ GVG +F+M++ K + +F D
Sbjct: 405 GLVIMCLIPSGFLIFVMAIATKTVYLVSAIMSVCGVGWFFLMKYCKSKKLFKFSTD 460
>ref|XP_003561594.1| PREDICTED: uncharacterized transporter lpg1691-like [Brachypodium
distachyon]
Length = 492
Score = 125 bits (315), Expect = 4e-27
Identities = 60/105 (57%), Positives = 77/105 (73%)
Frame = +1
Query: 25 TLLLSFVSFTNIVAAANFLYSCGMLLEFAAFLWLRRKRPDLKRPFRVPMEMRGLVVMCGV 204
TL +SF+SF NIVAAANFLYS GMLLEFA F+WLR KRPD+ RP+RVP+ + G V++C V
Sbjct: 366 TLGMSFLSFNNIVAAANFLYSLGMLLEFATFVWLRIKRPDMPRPYRVPLRLPGTVILCLV 425
Query: 205 PVCFLVAVMVMGNWVVYVLSVGLTALGVGAYFVMQWMKKNESMRF 339
P FLV VM + W VY +S TA GVG Y++M++ K ++F
Sbjct: 426 PSGFLVFVMAIAGWKVYAISAIFTAAGVGVYYLMKFCKARGFLKF 470
>gb|EXB75232.1| Arginine/agmatine antiporter [Morus notabilis]
Length = 490
Score = 125 bits (314), Expect = 6e-27
Identities = 60/124 (48%), Positives = 87/124 (70%)
Frame = +1
Query: 1 GILMSAGGTLLLSFVSFTNIVAAANFLYSCGMLLEFAAFLWLRRKRPDLKRPFRVPMEMR 180
GIL+S G L +S++ FT I+++ANFLYS GMLLEFA+F+WLRRK P LKRP++VPM +
Sbjct: 367 GILLSTGIVLAVSYLDFTVIISSANFLYSLGMLLEFASFIWLRRKMPALKRPYKVPMRLP 426
Query: 181 GLVVMCGVPVCFLVAVMVMGNWVVYVLSVGLTALGVGAYFVMQWMKKNESMRFVRDVACE 360
G+V+MC +P FL+ VM + +V+++S +TA G+G YF M++ K +F +
Sbjct: 427 GIVIMCLIPSGFLIFVMAIATKIVFLISGVMTAAGIGWYFFMKFCKARNWFKFNNNEFNP 486
Query: 361 DHDS 372
D DS
Sbjct: 487 DEDS 490
>ref|XP_006838223.1| hypothetical protein AMTR_s00217p00018260 [Amborella trichopoda]
gi|548840690|gb|ERN00792.1| hypothetical protein
AMTR_s00217p00018260 [Amborella trichopoda]
Length = 499
Score = 125 bits (313), Expect = 8e-27
Identities = 53/106 (50%), Positives = 82/106 (77%)
Frame = +1
Query: 1 GILMSAGGTLLLSFVSFTNIVAAANFLYSCGMLLEFAAFLWLRRKRPDLKRPFRVPMEMR 180
G+++ A ++ +SF+SF +I+++ANFLY CGM+LEFA+FLWLR ++ +++RP+RVPM M
Sbjct: 361 GVVVCAASSIAISFMSFNDIISSANFLYGCGMMLEFASFLWLRWRKGEVRRPYRVPMGMA 420
Query: 181 GLVVMCGVPVCFLVAVMVMGNWVVYVLSVGLTALGVGAYFVMQWMK 318
G+V +C VP F+V VMV+ +VY +S G+T +GVGAYF +W +
Sbjct: 421 GVVGLCLVPAAFIVFVMVLARKMVYAISAGVTVVGVGAYFFKRWCR 466
>ref|XP_002465208.1| hypothetical protein SORBIDRAFT_01g034150 [Sorghum bicolor]
gi|241919062|gb|EER92206.1| hypothetical protein
SORBIDRAFT_01g034150 [Sorghum bicolor]
Length = 496
Score = 125 bits (313), Expect = 8e-27
Identities = 64/123 (52%), Positives = 80/123 (65%)
Frame = +1
Query: 4 ILMSAGGTLLLSFVSFTNIVAAANFLYSCGMLLEFAAFLWLRRKRPDLKRPFRVPMEMRG 183
IL +A TL +SF+SF +IVAAANFLYS GMLLEFAAF+WLR K+P L RP+RVP + G
Sbjct: 359 ILATAAITLAMSFLSFNSIVAAANFLYSLGMLLEFAAFVWLRVKQPALARPYRVPARLPG 418
Query: 184 LVVMCGVPVCFLVAVMVMGNWVVYVLSVGLTALGVGAYFVMQWMKKNESMRFVRDVACED 363
V++C VP FLV VM + W VY +S TA GV Y++M+ K +RF D
Sbjct: 419 AVLLCLVPSVFLVFVMAIAGWKVYAISAAFTAAGVAVYYLMRLCKARGCLRFSDGAGAGD 478
Query: 364 HDS 372
S
Sbjct: 479 QGS 481
>dbj|BAJ96201.1| predicted protein [Hordeum vulgare subsp. vulgare]
Length = 488
Score = 124 bits (312), Expect = 1e-26
Identities = 59/105 (56%), Positives = 77/105 (73%)
Frame = +1
Query: 25 TLLLSFVSFTNIVAAANFLYSCGMLLEFAAFLWLRRKRPDLKRPFRVPMEMRGLVVMCGV 204
TL +SF SF NIVAAANFLYS GMLLEFA F+WLR KRP++ RP+RVP+ + G+VV+C V
Sbjct: 363 TLGMSFFSFNNIVAAANFLYSLGMLLEFATFVWLRIKRPEMSRPYRVPLRLPGIVVLCLV 422
Query: 205 PVCFLVAVMVMGNWVVYVLSVGLTALGVGAYFVMQWMKKNESMRF 339
P FLV VM + W VY +S TA G+G Y++M++ K ++F
Sbjct: 423 PSGFLVFVMAIAGWKVYAISAMFTAAGLGVYYLMKFCKARGFLKF 467
>dbj|BAJ85124.1| predicted protein [Hordeum vulgare subsp. vulgare]
Length = 486
Score = 124 bits (312), Expect = 1e-26
Identities = 59/105 (56%), Positives = 77/105 (73%)
Frame = +1
Query: 25 TLLLSFVSFTNIVAAANFLYSCGMLLEFAAFLWLRRKRPDLKRPFRVPMEMRGLVVMCGV 204
TL +SF SF NIVAAANFLYS GMLLEFA F+WLR KRP++ RP+RVP+ + G+VV+C V
Sbjct: 361 TLGMSFFSFNNIVAAANFLYSLGMLLEFATFVWLRIKRPEMSRPYRVPLRLPGIVVLCLV 420
Query: 205 PVCFLVAVMVMGNWVVYVLSVGLTALGVGAYFVMQWMKKNESMRF 339
P FLV VM + W VY +S TA G+G Y++M++ K ++F
Sbjct: 421 PSGFLVFVMAIAGWKVYAISAMFTAAGLGVYYLMKFCKARGFLKF 465
>gb|EMS64845.1| hypothetical protein TRIUR3_17687 [Triticum urartu]
Length = 157
Score = 124 bits (310), Expect = 2e-26
Identities = 58/105 (55%), Positives = 77/105 (73%)
Frame = +1
Query: 25 TLLLSFVSFTNIVAAANFLYSCGMLLEFAAFLWLRRKRPDLKRPFRVPMEMRGLVVMCGV 204
TL +SF SF NIVAAANFLYS GMLLEFA F+WLR KRP++ RP+RVP+ + G++V+C V
Sbjct: 32 TLGMSFFSFNNIVAAANFLYSLGMLLEFATFVWLRIKRPEMSRPYRVPLRLPGIIVLCLV 91
Query: 205 PVCFLVAVMVMGNWVVYVLSVGLTALGVGAYFVMQWMKKNESMRF 339
P FLV VM + W VY +S TA G+G Y++M++ K ++F
Sbjct: 92 PSGFLVFVMAIAGWKVYAISAMFTAAGLGVYYLMKFCKVRGFLKF 136
>ref|XP_006492719.1| PREDICTED: probable polyamine transporter At3g13620-like [Citrus
sinensis]
Length = 474
Score = 123 bits (309), Expect = 2e-26
Identities = 60/113 (53%), Positives = 85/113 (75%)
Frame = +1
Query: 1 GILMSAGGTLLLSFVSFTNIVAAANFLYSCGMLLEFAAFLWLRRKRPDLKRPFRVPMEMR 180
GIL+S TL +S++ F +IVA+ANFLYS GMLLEFAAF+WLR K+P LKRP+RVPM++
Sbjct: 353 GILVSTLITLGVSYMDFESIVASANFLYSLGMLLEFAAFIWLRMKKPQLKRPYRVPMKLP 412
Query: 181 GLVVMCGVPVCFLVAVMVMGNWVVYVLSVGLTALGVGAYFVMQWMKKNESMRF 339
GLV+MC +P FLV +MV+ +VY++S +T +G YF M+++K ++F
Sbjct: 413 GLVIMCLIPSGFLVVIMVVATKIVYLVSGLMTLGAIGWYFFMKFLKSKNVIKF 465
>gb|EMT21767.1| hypothetical protein F775_02768 [Aegilops tauschii]
Length = 239
Score = 123 bits (309), Expect = 2e-26
Identities = 58/105 (55%), Positives = 77/105 (73%)
Frame = +1
Query: 25 TLLLSFVSFTNIVAAANFLYSCGMLLEFAAFLWLRRKRPDLKRPFRVPMEMRGLVVMCGV 204
TL +SF SF NIVAAANFLYS GMLLEFA F+WLR KRP++ RP+RVP+ + G+VV+C V
Sbjct: 114 TLGMSFFSFNNIVAAANFLYSLGMLLEFATFVWLRIKRPEMSRPYRVPLRLPGIVVLCLV 173
Query: 205 PVCFLVAVMVMGNWVVYVLSVGLTALGVGAYFVMQWMKKNESMRF 339
P FLV VM + W VY +S TA G+G Y++M++ + ++F
Sbjct: 174 PSGFLVFVMAIAGWKVYAISAMFTAAGLGVYYLMKFCRARGFLKF 218
>gb|EMJ06341.1| hypothetical protein PRUPE_ppa005207mg [Prunus persica]
Length = 472
Score = 123 bits (309), Expect = 2e-26
Identities = 59/113 (52%), Positives = 85/113 (75%)
Frame = +1
Query: 1 GILMSAGGTLLLSFVSFTNIVAAANFLYSCGMLLEFAAFLWLRRKRPDLKRPFRVPMEMR 180
GIL+S TL +S+++FT+I+++ANFLYS GMLLEFA+F+WLRRK P L RP+RVPM++
Sbjct: 350 GILISTMITLAVSYMTFTDIISSANFLYSLGMLLEFASFIWLRRKLPALNRPYRVPMKLP 409
Query: 181 GLVVMCGVPVCFLVAVMVMGNWVVYVLSVGLTALGVGAYFVMQWMKKNESMRF 339
GLVVMC +P FL+ VM + VY++S +T G+G YF+M++ K + +F
Sbjct: 410 GLVVMCLIPSVFLIFVMAIATKTVYLISGVMTLGGIGWYFLMKFCKSKKLFKF 462
>gb|EOY32321.1| Amino acid permease family protein isoform 1 [Theobroma cacao]
gi|508785066|gb|EOY32322.1| Amino acid permease family
protein isoform 1 [Theobroma cacao]
Length = 479
Score = 121 bits (304), Expect = 8e-26
Identities = 54/113 (47%), Positives = 88/113 (77%)
Frame = +1
Query: 1 GILMSAGGTLLLSFVSFTNIVAAANFLYSCGMLLEFAAFLWLRRKRPDLKRPFRVPMEMR 180
GIL+S+ + +S+++FT+I+++ANFLYS GMLLEFA+F+WLRRK P++KRP+RVP+ +
Sbjct: 359 GILLSSFIAIGMSYMTFTDIISSANFLYSLGMLLEFASFIWLRRKLPEIKRPYRVPLRIP 418
Query: 181 GLVVMCGVPVCFLVAVMVMGNWVVYVLSVGLTALGVGAYFVMQWMKKNESMRF 339
GLV+MC +P FL+ VMV+ +V+++S +T +G YF+M++ +K + R+
Sbjct: 419 GLVIMCLIPSAFLIVVMVIATKIVFLVSGLMTVGAIGWYFLMKFCRKKKIFRY 471
>gb|EMT03109.1| hypothetical protein F775_15650 [Aegilops tauschii]
Length = 313
Score = 121 bits (304), Expect = 8e-26
Identities = 57/105 (54%), Positives = 75/105 (71%)
Frame = +1
Query: 25 TLLLSFVSFTNIVAAANFLYSCGMLLEFAAFLWLRRKRPDLKRPFRVPMEMRGLVVMCGV 204
TL +SF SF NIV AANFLYS GMLLEFA F+WLR KRP++ RP+RVP+ + G+VV+C V
Sbjct: 188 TLGMSFFSFNNIVTAANFLYSLGMLLEFATFIWLRIKRPEMSRPYRVPLRLPGIVVLCLV 247
Query: 205 PVCFLVAVMVMGNWVVYVLSVGLTALGVGAYFVMQWMKKNESMRF 339
P FLV VM + W Y +S TA G+G Y++M++ K ++F
Sbjct: 248 PSGFLVFVMAIAGWKAYAISAIFTAAGLGVYYLMKFCKARGFLKF 292
>ref|XP_004228661.1| PREDICTED: probable polyamine transporter At3g13620-like [Solanum
lycopersicum]
Length = 474
Score = 121 bits (304), Expect = 8e-26
Identities = 58/106 (54%), Positives = 78/106 (73%)
Frame = +1
Query: 1 GILMSAGGTLLLSFVSFTNIVAAANFLYSCGMLLEFAAFLWLRRKRPDLKRPFRVPMEMR 180
GIL+ L++S+++FT+IV++ NFLYS GMLLEFA+FLWLRRK P KRP+RVP+ M
Sbjct: 349 GILVPTLVALVVSYMTFTDIVSSVNFLYSLGMLLEFASFLWLRRKMPKAKRPYRVPLSMP 408
Query: 181 GLVVMCGVPVCFLVAVMVMGNWVVYVLSVGLTALGVGAYFVMQWMK 318
GLVVMC +P CFLV VM + + VY++S LT + YF M++ K
Sbjct: 409 GLVVMCFIPSCFLVYVMFVAHRTVYIVSAMLTVFAIAWYFFMEFCK 454
>ref|XP_006445660.1| hypothetical protein CICLE_v10015109mg [Citrus clementina]
gi|557548271|gb|ESR58900.1| hypothetical protein
CICLE_v10015109mg [Citrus clementina]
Length = 473
Score = 121 bits (303), Expect = 1e-25
Identities = 59/113 (52%), Positives = 84/113 (74%)
Frame = +1
Query: 1 GILMSAGGTLLLSFVSFTNIVAAANFLYSCGMLLEFAAFLWLRRKRPDLKRPFRVPMEMR 180
GIL+S TL +S++ F +IVA+ANFLYS GMLLEFAAF+WLR K+P LKRP+RVPM++
Sbjct: 353 GILVSTLITLGVSYMDFESIVASANFLYSLGMLLEFAAFIWLRMKKPQLKRPYRVPMKLP 412
Query: 181 GLVVMCGVPVCFLVAVMVMGNWVVYVLSVGLTALGVGAYFVMQWMKKNESMRF 339
GLV+MC +P FL +MV+ +VY++S +T +G YF M+++K ++F
Sbjct: 413 GLVIMCLIPSGFLFVIMVVATKIVYLVSGLMTLGAIGWYFFMKFLKAKNVIKF 465