BLASTX nr result
ID: Ephedra26_contig00006826
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Ephedra26_contig00006826
(2167 letters)
Database: ./nr
37,332,560 sequences; 13,225,080,153 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|ABV44454.1| longifolene synthase [Pinus sylvestris] 153 4e-70
gb|ACN39765.1| unknown [Picea sitchensis] gi|325930026|gb|ADZ455... 159 4e-69
sp|Q675L0.1|TPSLS_PICAB RecName: Full=Longifolene synthase; Shor... 157 8e-69
gb|ABA86249.1| delta-selinene-like synthase [Picea sitchensis] 160 1e-68
gb|ADZ45515.1| longifolene synthase [Picea engelmannii x Picea g... 158 1e-68
gb|AAK39129.2|AF369920_1 gamma-humulene synthase-like protein [P... 156 2e-68
gb|ABV44453.1| 1(10),5-germacradien-4-ol synthase [Pinus sylvest... 151 3e-68
gb|ACN40827.1| unknown [Picea sitchensis] 157 5e-68
gb|ADZ45513.1| alpha-humulene synthase [Picea glauca] 157 3e-66
gb|ABW80964.1| alpha pinene synthase [Chamaecyparis formosensis] 125 6e-59
gb|ADX42738.1| (E)-beta-farnesene synthase 2 [Pseudotsuga menzie... 147 1e-58
gb|ADX42737.1| (E)-beta-farnesene synthase 1 [Pseudotsuga menzie... 147 1e-58
sp|O64405.1|TPSD5_ABIGR RecName: Full=Gamma-humulene synthase; A... 225 7e-56
gb|ADH29869.1| e-beta farnesene synthase [Pinus sylvestris] 149 3e-55
dbj|BAF43701.1| putative sesquiterpene synthase [Cycas taitungen... 137 4e-54
gb|ABV44452.1| caryophyllene/humulene synthase [Pinus sylvestris] 167 1e-53
gb|AAS47689.1| E-alpha-bisabolene synthase [Picea abies] 132 3e-53
sp|H8ZM73.1|CAS_ABIBA RecName: Full=Bifunctional cis-abienol syn... 132 1e-52
gb|AGN70885.1| levopimaradiene/abietadiene synthase, partial [Ps... 134 3e-52
sp|Q675L4.1|TPSD1_PICAB RecName: Full=Levopimaradiene synthase, ... 131 6e-52
>gb|ABV44454.1| longifolene synthase [Pinus sylvestris]
Length = 580
Score = 153 bits (387), Expect(3) = 4e-70
Identities = 107/341 (31%), Positives = 167/341 (48%), Gaps = 3/341 (0%)
Frame = +2
Query: 914 LNMKILQLAKLDYNIVQAKLQKDLKEVSRWWNNTEISKLRNFRDGLVRSFLIPACGIYEP 1093
+N K+L+LAKLD+NI+Q+ Q++L+ +SRWW+ ++I K +R V + G +EP
Sbjct: 252 MNKKVLELAKLDFNILQSAHQRELQLLSRWWSQSDIEKQNFYRKRHVEFYFWMVIGTFEP 311
Query: 1094 ELSGVRILTTKGCLGSYILDDVYDEFASQEELPIIIKAMEKLKLDANNGYCLKEHIITGC 1273
E S RI K ILDD+YD + E+L I +A+++ L + L ++I
Sbjct: 312 EFSSSRIAFAKIATLMTILDDLYDTHGTLEQLKIFTEAVKRWDLSLQDR--LPDYIKITL 369
Query: 1274 QFYLDANIQIITELNNIQGADTQKYLHQRIMGWAHASE---QEAIWKFQGYVPTFEEYMR 1444
+F+ + + ++ E+ +Q D Y+ R GW E QE+ W +VPTF++YM+
Sbjct: 370 EFFFNTSNELNAEVAKMQERDMSAYI--RKAGWERYIEGYMQESEWMAARHVPTFDDYMK 427
Query: 1445 QGVESVAMLPLFLVAKLGLCSGFPLADLEMRPHGISTFDKLCALSCRLTDDIHDFAEEKE 1624
G S M L L + L + P LE + H S +L L+ RL DD DF +K+
Sbjct: 428 NGKRSSGMCILNLYSLLLMGQLVPDNILE-QIHLPSKIHELVELTARLVDDSKDFQAKKD 486
Query: 1625 QGFNISAVESYAKDNPECTLEEVVAFLRDMTQGFMKNIVQHYFSTHTAPEQSQTPEDSFY 1804
G S E Y K+ PECT E+ + L + + F H +
Sbjct: 487 GGEFASGTECYLKEKPECTEEDAMNHLIGLL-NLTAMELNWEFVKHDGV--------ALC 537
Query: 1805 FKRTTLEMTRTFNFFYSYGDGFGHLNDGLINQIDAILFQPI 1927
K+ E+ R F Y Y DGF + N+ + +QI IL +
Sbjct: 538 LKKFVFEVARGLRFIYKYRDGFDYSNEEMKSQITKILIDQV 578
Score = 91.7 bits (226), Expect(3) = 4e-70
Identities = 51/122 (41%), Positives = 74/122 (60%), Gaps = 1/122 (0%)
Frame = +2
Query: 116 WNDSFIQSITSPFSDPLQGEKADNLTKKIKWGIMEAFDKCGAENDLIERLSLIDCLLCLG 295
W+D FIQS+ SP+ P E+ + + +++K ++ + +DLI+RL ++D CLG
Sbjct: 28 WDDYFIQSLKSPYEAPECHERCEKMIEEVKHLLLS--EMRDGNDDLIKRLQMVDIFECLG 85
Query: 296 IDRLFQEEIRRDLDYVYMKAWNSSSGIGDGTK-SPKANIVSTALAFKLLRLHRYQNVSPV 472
IDR F EI+ LDYVY + WN GIG GT+ S ++ +T L F+ LRLHRY S V
Sbjct: 86 IDRHFHHEIQAALDYVY-RYWNELEGIGVGTRDSLTKDLYATGLGFRALRLHRYNVSSAV 144
Query: 473 SE 478
E
Sbjct: 145 LE 146
Score = 70.5 bits (171), Expect(3) = 4e-70
Identities = 39/114 (34%), Positives = 62/114 (54%)
Frame = +3
Query: 471 FQNGSGHGFLEEEISSSLFTRTSVAKALNLVRASYLQFHGETILEEAKTSCTSFLNSFIS 650
F+N +G F SS V L L+RAS + F GE +++EAK T +LN ++
Sbjct: 148 FKNENGLFF-----HSSAVQEEEVRCMLTLLRASEISFPGEKVMDEAKAFATEYLNQLLT 202
Query: 651 LQQNVTESKDDFARLKQEVEHTLQYQWILTTPRWEARRFIDFYSANKDWLRYSL 812
+ ++TE ++ R EV + L + W + PRWEAR FI+ + N WL+ ++
Sbjct: 203 -RVDITEVGENLLR---EVRYALDFPWYCSVPRWEARSFIEIFGQNNSWLKSTM 252
>gb|ACN39765.1| unknown [Picea sitchensis] gi|325930026|gb|ADZ45516.1|
alpha-longipinene synthase [Picea sitchensis]
Length = 579
Score = 159 bits (402), Expect(3) = 4e-69
Identities = 105/339 (30%), Positives = 165/339 (48%), Gaps = 1/339 (0%)
Frame = +2
Query: 914 LNMKILQLAKLDYNIVQAKLQKDLKEVSR-WWNNTEISKLRNFRDGLVRSFLIPACGIYE 1090
+N ++L+LAKLD+NI+Q ++L+ +SR WW+ ++I++ +R V + G +E
Sbjct: 252 MNKRVLELAKLDFNILQCAHHRELQLLSRCWWSQSDIAQQNFYRKRHVEFYFWVVIGTFE 311
Query: 1091 PELSGVRILTTKGCLGSYILDDVYDEFASQEELPIIIKAMEKLKLDANNGYCLKEHIITG 1270
PE S RI K ILDD+YD + E+L I + +++ L + L ++I
Sbjct: 312 PEFSTCRITFAKIATLMTILDDLYDTHGTLEQLKIFTEGVKRWDLSLVDS--LPDYIKIT 369
Query: 1271 CQFYLDANIQIITELNNIQGADTQKYLHQRIMGWAHASEQEAIWKFQGYVPTFEEYMRQG 1450
+F+L+ + ++I E+ Q D Y+ + + A QEA W G+VPTF+EYM+ G
Sbjct: 370 FEFFLNTSNELIAEVAKTQERDMSAYIRKTWERYLEAYMQEAEWITAGHVPTFDEYMKNG 429
Query: 1451 VESVAMLPLFLVAKLGLCSGFPLADLEMRPHGISTFDKLCALSCRLTDDIHDFAEEKEQG 1630
+ S M L L + L + P D+ + H S +L L+ RL DD DF K G
Sbjct: 430 ISSSGMCILNLYSLLLMGQLLP-DDVLEQIHSPSKIHELVELTARLVDDSKDFETNKVGG 488
Query: 1631 FNISAVESYAKDNPECTLEEVVAFLRDMTQGFMKNIVQHYFSTHTAPEQSQTPEDSFYFK 1810
S +E Y KDNPECTLE+ L + +K + + + + FK
Sbjct: 489 ELASGIECYVKDNPECTLEDASNHLNGLLDLTVKELNWEFVRHDSV---------ALCFK 539
Query: 1811 RTTLEMTRTFNFFYSYGDGFGHLNDGLINQIDAILFQPI 1927
+ + R Y Y DGF N + I IL P+
Sbjct: 540 KFAFNVARGLRLIYKYRDGFDVSNQEMKTHIFKILIDPL 578
Score = 90.5 bits (223), Expect(3) = 4e-69
Identities = 54/122 (44%), Positives = 72/122 (59%), Gaps = 1/122 (0%)
Frame = +2
Query: 116 WNDSFIQSITSPFSDPLQGEKADNLTKKIKWGIMEAFDKCGAENDLIERLSLIDCLLCLG 295
W+D FIQS+ S P E+A+ L ++IK ++ C +DLI L ++D CLG
Sbjct: 28 WDDDFIQSLKSSNGAPQYHERAEKLVEEIKNLVVSEMKDCN--DDLIRHLQMVDIFECLG 85
Query: 296 IDRLFQEEIRRDLDYVYMKAWNSSSGIGDGTK-SPKANIVSTALAFKLLRLHRYQNVSPV 472
IDR FQ EI+ LDYVY + WN GIG G++ S + +TAL F+ LRLHRY S V
Sbjct: 86 IDRHFQNEIQVALDYVY-RYWNELEGIGIGSRDSLIKDFNATALGFRALRLHRYNVSSDV 144
Query: 473 SE 478
E
Sbjct: 145 LE 146
Score = 62.8 bits (151), Expect(3) = 4e-69
Identities = 38/111 (34%), Positives = 56/111 (50%)
Frame = +3
Query: 471 FQNGSGHGFLEEEISSSLFTRTSVAKALNLVRASYLQFHGETILEEAKTSCTSFLNSFIS 650
F+N +G F SS V L L RAS + F GE +++EAK T +L ++
Sbjct: 148 FKNENGQFFC-----SSTVEEKEVRCMLTLFRASEISFPGEKVMDEAKAFTTEYLTKVLT 202
Query: 651 LQQNVTESKDDFARLKQEVEHTLQYQWILTTPRWEARRFIDFYSANKDWLR 803
+VT+ R EV++ L++ W + PRWEAR FI+ N WL+
Sbjct: 203 -GVDVTDVDQSLLR---EVKYALEFPWHCSLPRWEARNFIEICGQNDSWLK 249
>sp|Q675L0.1|TPSLS_PICAB RecName: Full=Longifolene synthase; Short=PaTPS-Lon
gi|44804575|gb|AAS47695.1| longifolene synthase [Picea
abies]
Length = 578
Score = 157 bits (398), Expect(3) = 8e-69
Identities = 104/338 (30%), Positives = 165/338 (48%)
Frame = +2
Query: 914 LNMKILQLAKLDYNIVQAKLQKDLKEVSRWWNNTEISKLRNFRDGLVRSFLIPACGIYEP 1093
+N ++L+LAKLD+NI+Q ++L+ +S WW+ ++I++ +R V +L G +EP
Sbjct: 252 MNKRVLELAKLDFNILQWAHHRELQLLSSWWSQSDIAQQNFYRKRHVEFYLWVVIGTFEP 311
Query: 1094 ELSGVRILTTKGCLGSYILDDVYDEFASQEELPIIIKAMEKLKLDANNGYCLKEHIITGC 1273
E S RI K ILDD+YD + E+L I + +++ L + L ++I
Sbjct: 312 EFSTCRITFAKISTLMTILDDLYDTHGTLEQLKIFTEGVKRWDLSLVDR--LPDYIKITF 369
Query: 1274 QFYLDANIQIITELNNIQGADTQKYLHQRIMGWAHASEQEAIWKFQGYVPTFEEYMRQGV 1453
+F+L+ + ++I E+ Q D Y+ + + A QEA W +VPTF+EYM+ G+
Sbjct: 370 EFFLNTSNELIAEVAKTQERDMSAYIRKTWERYLEAYLQEAEWIAARHVPTFDEYMKNGI 429
Query: 1454 ESVAMLPLFLVAKLGLCSGFPLADLEMRPHGISTFDKLCALSCRLTDDIHDFAEEKEQGF 1633
S M L L + L + P D+ + H S +L L+ RL DD DF +K G
Sbjct: 430 SSSGMCILNLYSLLLMGQLLP-DDVLEQIHSPSKIHELVELTARLVDDSKDFETKKVGGE 488
Query: 1634 NISAVESYAKDNPECTLEEVVAFLRDMTQGFMKNIVQHYFSTHTAPEQSQTPEDSFYFKR 1813
S +E Y KDNPECTLE+ L + +K + + + + FK+
Sbjct: 489 LASGIECYVKDNPECTLEDASNHLNGLLDLTVKELNWEFVRHDSV---------ALCFKK 539
Query: 1814 TTLEMTRTFNFFYSYGDGFGHLNDGLINQIDAILFQPI 1927
+ R Y Y DGF N + I IL P+
Sbjct: 540 FAFNVARGLRLIYKYRDGFDVSNQEMKTHIFKILIDPL 577
Score = 90.9 bits (224), Expect(3) = 8e-69
Identities = 55/122 (45%), Positives = 72/122 (59%), Gaps = 1/122 (0%)
Frame = +2
Query: 116 WNDSFIQSITSPFSDPLQGEKADNLTKKIKWGIMEAFDKCGAENDLIERLSLIDCLLCLG 295
W+D FIQS+ S P E+A L ++IK ++ C +DLI RL ++D CLG
Sbjct: 28 WDDDFIQSLKSSNGAPQYHERAAKLVEEIKNLVVSEMKDCN--DDLIRRLQMVDIFECLG 85
Query: 296 IDRLFQEEIRRDLDYVYMKAWNSSSGIGDGTK-SPKANIVSTALAFKLLRLHRYQNVSPV 472
IDR FQ EI+ LDYVY + WN GIG G++ S + +TAL F+ LRLHRY S V
Sbjct: 86 IDRHFQHEIQVALDYVY-RYWNQLEGIGIGSRDSLIKDFNATALGFRALRLHRYNVSSDV 144
Query: 473 SE 478
E
Sbjct: 145 LE 146
Score = 62.8 bits (151), Expect(3) = 8e-69
Identities = 38/111 (34%), Positives = 56/111 (50%)
Frame = +3
Query: 471 FQNGSGHGFLEEEISSSLFTRTSVAKALNLVRASYLQFHGETILEEAKTSCTSFLNSFIS 650
F+N +G F SS V L L RAS + F GE +++EAK T +L ++
Sbjct: 148 FKNENGQFFC-----SSTVEEKEVRCMLTLFRASEISFPGEKVMDEAKAFTTEYLTKVLT 202
Query: 651 LQQNVTESKDDFARLKQEVEHTLQYQWILTTPRWEARRFIDFYSANKDWLR 803
+VT+ R EV++ L++ W + PRWEAR FI+ N WL+
Sbjct: 203 -GVDVTDVNQSLLR---EVKYALEFPWHCSLPRWEARSFIEICGQNDSWLK 249
>gb|ABA86249.1| delta-selinene-like synthase [Picea sitchensis]
Length = 575
Score = 160 bits (405), Expect(3) = 1e-68
Identities = 107/345 (31%), Positives = 169/345 (48%), Gaps = 6/345 (1%)
Frame = +2
Query: 911 NLNMKILQLAKLDYNIVQAKLQKDLKEVSRWWNNTEISKLRNFRDGLVRSFLIPACGIYE 1090
N+N K+L+LAKLD+NI+Q QK+++ + RWW +EI++L +R V + I+E
Sbjct: 245 NINQKVLELAKLDFNILQCIHQKEIQFIVRWWRESEIAQLNFYRKRHVEFYFWVVICIFE 304
Query: 1091 PELSGVRILTTKGCLGSYILDDVYDEFASQEELPIIIKAMEKLKLDANNGYCLKEHIITG 1270
PE S RI K C + +LDD+YD +EL + + + + L + L ++I
Sbjct: 305 PEFSQSRIAFAKICTVATVLDDLYDTHGMLDELKTVTEGVSRWDLPLIDD--LPDNIKIA 362
Query: 1271 CQFYLDANIQIITELNNIQGADTQKYLHQRIMGWAHASEQEAIWKFQGYVPTFEEYMRQG 1450
QF+ + ++ E+ QG D L + + QEA W +VP+F+EY++
Sbjct: 363 FQFFFNTANELAVEVVKKQGRDMIALLKANWQRYVESYLQEAEWIATRHVPSFDEYIKNA 422
Query: 1451 VESVAM-----LPLFLVAKLGLCSGFPLA-DLEMRPHGISTFDKLCALSCRLTDDIHDFA 1612
S M +PL L+ +L LA ++ + H S +L L+ RL DDI DF
Sbjct: 423 RASSGMCIGNLIPLLLLGQL-------LANNIVEQIHSPSKIQELSELTIRLIDDIRDFE 475
Query: 1613 EEKEQGFNISAVESYAKDNPECTLEEVVAFLRDMTQGFMKNIVQHYFSTHTAPEQSQTPE 1792
+EKE+G SA+E Y KDNP+ T+E + L + ++ + + T P
Sbjct: 476 DEKERGEIASAIECYMKDNPDSTVENALNHLEGILHLSLEELNWEFIKQDTVP------- 528
Query: 1793 DSFYFKRTTLEMTRTFNFFYSYGDGFGHLNDGLINQIDAILFQPI 1927
K+ T + R F Y YGDG N + +QI IL +
Sbjct: 529 --LCCKKFTFNIVRGLQFVYKYGDGLSISNKEVKDQIFKILIDQV 571
Score = 83.2 bits (204), Expect(3) = 1e-68
Identities = 52/123 (42%), Positives = 73/123 (59%), Gaps = 2/123 (1%)
Frame = +2
Query: 116 WNDSFIQSITSPFSDPLQGEKADNLTKKIKWGIMEAFDKCGAENDLIERLSLIDCLLCLG 295
W+D I S+ SP+ P E+ NL +++K ++ + + +DLI RL ++D L CLG
Sbjct: 22 WDDDLILSLDSPYGAPAYYERVANLIEEMKHLLLREMED--SNHDLIRRLQIVDTLECLG 79
Query: 296 IDRLFQEEIR-RDLDYVYMKAWNSSSGIGDGTK-SPKANIVSTALAFKLLRLHRYQNVSP 469
IDR FQ EI+ LDYVY + WN GIG G+ S ++ +TAL + LRLHRY S
Sbjct: 80 IDRHFQHEIKTAALDYVY-RCWN-EKGIGMGSSDSGSKDLDATALGLRALRLHRYNVSSG 137
Query: 470 VSE 478
V E
Sbjct: 138 VLE 140
Score = 67.4 bits (163), Expect(3) = 1e-68
Identities = 32/91 (35%), Positives = 53/91 (58%)
Frame = +3
Query: 540 VAKALNLVRASYLQFHGETILEEAKTSCTSFLNSFISLQQNVTESKDDFARLKQEVEHTL 719
V L+L+RAS + F GE +++EAK +L ++ + +VT+ R EV++ L
Sbjct: 159 VRSMLSLLRASEISFPGEKVMQEAKAFTREYLTQVLAGRGDVTDVDQSLLR---EVKYAL 215
Query: 720 QYQWILTTPRWEARRFIDFYSANKDWLRYSL 812
++ W + PRWEAR FI+ Y N WL+ ++
Sbjct: 216 EFPWHWSVPRWEARSFIEIYGQNHSWLKSNI 246
>gb|ADZ45515.1| longifolene synthase [Picea engelmannii x Picea glauca]
Length = 578
Score = 158 bits (400), Expect(3) = 1e-68
Identities = 104/338 (30%), Positives = 165/338 (48%)
Frame = +2
Query: 914 LNMKILQLAKLDYNIVQAKLQKDLKEVSRWWNNTEISKLRNFRDGLVRSFLIPACGIYEP 1093
+N ++L+LAKLD+NI+Q ++L+ +S WW+ ++I++ +R V +L G +EP
Sbjct: 252 MNKRVLELAKLDFNILQCAHHRELQLLSSWWSQSDIAQQNFYRKRHVEYYLWVVIGTFEP 311
Query: 1094 ELSGVRILTTKGCLGSYILDDVYDEFASQEELPIIIKAMEKLKLDANNGYCLKEHIITGC 1273
E S RI K ILDD+YD + E+L I + +++ L + L ++I
Sbjct: 312 EFSTCRIAFAKIATLMTILDDLYDTHGTLEQLKIFTEGVKRWDLSLVDR--LPDYIKITF 369
Query: 1274 QFYLDANIQIITELNNIQGADTQKYLHQRIMGWAHASEQEAIWKFQGYVPTFEEYMRQGV 1453
+F+L+ + ++I E+ Q D Y+ + + A QEA W +VPTF+EYM+ G+
Sbjct: 370 EFFLNTSNELIAEVAKTQERDMSAYIRKTWERYLEAYLQEAEWIAARHVPTFDEYMKNGI 429
Query: 1454 ESVAMLPLFLVAKLGLCSGFPLADLEMRPHGISTFDKLCALSCRLTDDIHDFAEEKEQGF 1633
S M L L + L + P D+ + H S +L L+ RL DD DF +K G
Sbjct: 430 SSSGMCILNLYSLLLMGQLLP-DDVLEQIHSPSKIHELVELTARLVDDSKDFETKKAGGE 488
Query: 1634 NISAVESYAKDNPECTLEEVVAFLRDMTQGFMKNIVQHYFSTHTAPEQSQTPEDSFYFKR 1813
S +E Y KDNPECTLE+ L + +K + + + + FK+
Sbjct: 489 LASGIECYVKDNPECTLEDASNHLIGLLDLTVKELNWEFVRHDSV---------ALCFKK 539
Query: 1814 TTLEMTRTFNFFYSYGDGFGHLNDGLINQIDAILFQPI 1927
+ R Y Y DGF N + I IL P+
Sbjct: 540 FAFNVARGLRLIYKYRDGFDVSNQEMKTHIFKILIDPL 577
Score = 89.7 bits (221), Expect(3) = 1e-68
Identities = 54/122 (44%), Positives = 72/122 (59%), Gaps = 1/122 (0%)
Frame = +2
Query: 116 WNDSFIQSITSPFSDPLQGEKADNLTKKIKWGIMEAFDKCGAENDLIERLSLIDCLLCLG 295
W D FIQS+ S P ++A+ L ++IK ++ C +DLI RL ++D CLG
Sbjct: 28 WADDFIQSLKSSNGAPQYHKRAEKLVEEIKNLVVSEMKDCN--DDLIRRLQMVDIFECLG 85
Query: 296 IDRLFQEEIRRDLDYVYMKAWNSSSGIGDGTK-SPKANIVSTALAFKLLRLHRYQNVSPV 472
IDR FQ EI+ LDYVY + WN GIG G++ S + +TAL F+ LRLHRY S V
Sbjct: 86 IDRHFQHEIQVALDYVY-RYWNELEGIGIGSRDSLIKDFNATALGFRALRLHRYNVSSDV 144
Query: 473 SE 478
E
Sbjct: 145 LE 146
Score = 62.4 bits (150), Expect(3) = 1e-68
Identities = 38/111 (34%), Positives = 56/111 (50%)
Frame = +3
Query: 471 FQNGSGHGFLEEEISSSLFTRTSVAKALNLVRASYLQFHGETILEEAKTSCTSFLNSFIS 650
F+N +G F SS V L L RAS + F GE +++EAK T +L ++
Sbjct: 148 FKNENGQFFC-----SSTVEEKEVRCMLTLFRASEISFPGEKVMDEAKAFTTEYLTKVLT 202
Query: 651 LQQNVTESKDDFARLKQEVEHTLQYQWILTTPRWEARRFIDFYSANKDWLR 803
+VT+ R EV++ L++ W + PRWEAR FI+ N WL+
Sbjct: 203 -GVDVTDVDQSLLR---EVKYALEFPWHCSLPRWEARSFIEICGQNDSWLK 249
>gb|AAK39129.2|AF369920_1 gamma-humulene synthase-like protein [Picea abies]
Length = 577
Score = 156 bits (395), Expect(3) = 2e-68
Identities = 105/338 (31%), Positives = 165/338 (48%)
Frame = +2
Query: 914 LNMKILQLAKLDYNIVQAKLQKDLKEVSRWWNNTEISKLRNFRDGLVRSFLIPACGIYEP 1093
+N ++L+LAKLD+NI+Q ++L+ +S WW+ ++I++ +R V +L G +EP
Sbjct: 252 MNKRVLELAKLDFNILQCAHHRELQLLSSWWSQSDIAQQNFYRKRHVEFYLWVVIGTFEP 311
Query: 1094 ELSGVRILTTKGCLGSYILDDVYDEFASQEELPIIIKAMEKLKLDANNGYCLKEHIITGC 1273
E S RI K ILDD+YD + E+L I + +++ L + L ++I
Sbjct: 312 EFSTCRITFAKIATLMTILDDLYDTHGTLEQLKIFTEGVKRWDLSLVDR--LPDYIKITF 369
Query: 1274 QFYLDANIQIITELNNIQGADTQKYLHQRIMGWAHASEQEAIWKFQGYVPTFEEYMRQGV 1453
+F+L+ + ++I E+ Q D Y+ + + A QEA W +VPTF+EYM+ G+
Sbjct: 370 EFFLNTSNELIAEVAKTQERDMSAYIRKTWERYLEAYLQEAEWIAARHVPTFDEYMKNGI 429
Query: 1454 ESVAMLPLFLVAKLGLCSGFPLADLEMRPHGISTFDKLCALSCRLTDDIHDFAEEKEQGF 1633
S M L L + L + P D+ + H S +L L+ RL DD DF E K G
Sbjct: 430 SSSGMCILNLYSLLLMGQLLP-DDVLEQIHSPSKIHELVELTARLVDDSKDF-ETKVGGE 487
Query: 1634 NISAVESYAKDNPECTLEEVVAFLRDMTQGFMKNIVQHYFSTHTAPEQSQTPEDSFYFKR 1813
S +E Y KDNPECTLE+ L + +K + + + + FK+
Sbjct: 488 LASGIECYVKDNPECTLEDASNHLNGLLDLTVKELNWEFVRHDSV---------ALCFKK 538
Query: 1814 TTLEMTRTFNFFYSYGDGFGHLNDGLINQIDAILFQPI 1927
+ R Y Y DGF N + I IL P+
Sbjct: 539 FAFNVARGLRLIYKYRDGFDVSNQEMKTHIFKILIDPL 576
Score = 90.9 bits (224), Expect(3) = 2e-68
Identities = 55/122 (45%), Positives = 72/122 (59%), Gaps = 1/122 (0%)
Frame = +2
Query: 116 WNDSFIQSITSPFSDPLQGEKADNLTKKIKWGIMEAFDKCGAENDLIERLSLIDCLLCLG 295
W+D FIQS+ S P E+A L ++IK ++ C +DLI RL ++D CLG
Sbjct: 28 WDDDFIQSLKSSNGAPQYHERAAKLVEEIKNLVVSEMKDCN--DDLIRRLQMVDIFECLG 85
Query: 296 IDRLFQEEIRRDLDYVYMKAWNSSSGIGDGTK-SPKANIVSTALAFKLLRLHRYQNVSPV 472
IDR FQ EI+ LDYVY + WN GIG G++ S + +TAL F+ LRLHRY S V
Sbjct: 86 IDRHFQHEIQVALDYVY-RYWNQLEGIGIGSRDSLIKDFNATALGFRALRLHRYNVSSDV 144
Query: 473 SE 478
E
Sbjct: 145 LE 146
Score = 62.4 bits (150), Expect(3) = 2e-68
Identities = 38/111 (34%), Positives = 56/111 (50%)
Frame = +3
Query: 471 FQNGSGHGFLEEEISSSLFTRTSVAKALNLVRASYLQFHGETILEEAKTSCTSFLNSFIS 650
F+N +G F SS V L L RAS + F GE +++EAK T +L ++
Sbjct: 148 FKNENGQFFC-----SSTVEEKEVRCMLTLFRASEISFPGEKVMDEAKAFTTEYLTKVLT 202
Query: 651 LQQNVTESKDDFARLKQEVEHTLQYQWILTTPRWEARRFIDFYSANKDWLR 803
+VT+ R EV++ L++ W + PRWEAR FI+ N WL+
Sbjct: 203 -GVDVTDVDQSLLR---EVKYALEFPWHCSLPRWEARSFIEICGQNDSWLK 249
>gb|ABV44453.1| 1(10),5-germacradien-4-ol synthase [Pinus sylvestris]
Length = 625
Score = 151 bits (382), Expect(3) = 3e-68
Identities = 101/341 (29%), Positives = 166/341 (48%), Gaps = 3/341 (0%)
Frame = +2
Query: 914 LNMKILQLAKLDYNIVQAKLQKDLKEVSRWWNNTEISKLRNFRDGLVRSFLIPACGIYEP 1093
+N KIL+LAKLD+NI+Q +++++ +SRW + + + +L +R V + G +EP
Sbjct: 297 MNQKILELAKLDFNILQCTHKEEMQLISRWRSESYLPQLDFYRKRHVELYFWAVLGTFEP 356
Query: 1094 ELSGVRILTTKGCLGSYILDDVYDEFASQEELPIIIKAMEKLKLDANNGYCLKEHIITGC 1273
E RI TK ++DD+YD + +E+ I + + + D + L +HI
Sbjct: 357 EFRSSRIAFTKLSTVMTVIDDLYDTHGTLDEIKIFTEGVR--RWDTSLISRLPDHIQKIF 414
Query: 1274 QFYLDANIQIITELNNIQGADTQKYLHQRIMGWAHASE---QEAIWKFQGYVPTFEEYMR 1444
+F++ + + E+ QG D Y+ + GW E QE W GYVP+F EY +
Sbjct: 415 EFFMKTSNEWTAEVEKKQGRDMAAYIRKN--GWERYVESYLQEGEWMAAGYVPSFNEYYK 472
Query: 1445 QGVESVAMLPLFLVAKLGLCSGFPLADLEMRPHGISTFDKLCALSCRLTDDIHDFAEEKE 1624
G+ S M L L+ L + P D+ + S +L L+ R+ DDI DF +EKE
Sbjct: 473 NGLASSGMCVLNLIPLLLMDQILP-DDILKQIVYPSKIHELLELTIRVKDDITDFEKEKE 531
Query: 1625 QGFNISAVESYAKDNPECTLEEVVAFLRDMTQGFMKNIVQHYFSTHTAPEQSQTPEDSFY 1804
G S E Y KDNPECT E+ + ++ + + + + P
Sbjct: 532 HGQVASCSECYMKDNPECTREDALNHMKGILDLSVSQLNWEFLKHDNVP---------LC 582
Query: 1805 FKRTTLEMTRTFNFFYSYGDGFGHLNDGLINQIDAILFQPI 1927
+KR T + R +F + Y DG ++ + +QI +L QP+
Sbjct: 583 YKRFTFNLARGMHFLFKYNDGITLSDNEVKDQIFKVLIQPL 623
Score = 87.0 bits (214), Expect(3) = 3e-68
Identities = 51/124 (41%), Positives = 77/124 (62%), Gaps = 3/124 (2%)
Frame = +2
Query: 116 WNDSFIQSITSPFSDPLQGEKADNLTKKIKWGIMEAF-DKCG-AENDLIERLSLIDCLLC 289
W+D I S+++ ++ P E+ + L + IK ++ D C A +DLI RL ++D + C
Sbjct: 65 WDDDVIHSLSTSYAAPTYRERGETLVEDIKHRLLNDMKDSCSDAADDLIRRLQMVDIIEC 124
Query: 290 LGIDRLFQEEIRRDLDYVYMKAWNSSSGIGDGTK-SPKANIVSTALAFKLLRLHRYQNVS 466
LGIDR FQ EI+ +DYVY + WN +GIG G++ S ++ +TAL F+ LR+HRY S
Sbjct: 125 LGIDRHFQPEIKEAIDYVY-RYWN-ETGIGLGSRNSGIKDLNATALGFRALRMHRYNVSS 182
Query: 467 PVSE 478
V E
Sbjct: 183 DVLE 186
Score = 70.9 bits (172), Expect(3) = 3e-68
Identities = 38/111 (34%), Positives = 57/111 (51%)
Frame = +3
Query: 471 FQNGSGHGFLEEEISSSLFTRTSVAKALNLVRASYLQFHGETILEEAKTSCTSFLNSFIS 650
F++ SG F V L+L RAS + F GE ++EEAKT T +L ++
Sbjct: 188 FKDESGQFFCSSSTGEEGNADKEVRSMLSLFRASNISFPGEKVMEEAKTFTTQYLTQVLT 247
Query: 651 LQQNVTESKDDFARLKQEVEHTLQYQWILTTPRWEARRFIDFYSANKDWLR 803
+ D L++EV++ L++ W + PRWEAR FI+ Y N WL+
Sbjct: 248 GHT----AADVDQSLQREVKYALEFPWHCSVPRWEARNFIEIYEQNYSWLK 294
>gb|ACN40827.1| unknown [Picea sitchensis]
Length = 654
Score = 157 bits (398), Expect(3) = 5e-68
Identities = 104/342 (30%), Positives = 173/342 (50%), Gaps = 3/342 (0%)
Frame = +2
Query: 911 NLNMKILQLAKLDYNIVQAKLQKDLKEVSRWWNNTEISKLRNFRDGLVRSFLIPACGIYE 1090
N+N KIL+LAKLD+NI+Q QK+++ +SRWW ++ + +L +R V + G +E
Sbjct: 325 NINAKILELAKLDFNILQCTHQKEMQLISRWWADSYLPQLGFYRKRHVELYFWVVTGTFE 384
Query: 1091 PELSGVRILTTKGCLGSYILDDVYDEFASQEELPIIIKAMEKLKLDANNGYCLKEHIITG 1270
PE S RI TK + +LDD+YD + +E+ I + + + D + L +HI
Sbjct: 385 PEFSSSRIAFTKIATLATVLDDLYDTHGTLDEVKIFTEGVR--RWDNSLIGRLPDHIKKI 442
Query: 1271 CQFYLDANIQIITELNNIQGADTQKYLHQRIMGWAHASE---QEAIWKFQGYVPTFEEYM 1441
+F++ + ++ E+ QG D + Y+ + GW E QEA W GYVP+F EY+
Sbjct: 443 FEFFMKTSNEMNDEVEKKQGRDMRAYIRKN--GWERYLESYLQEAEWIAAGYVPSFNEYL 500
Query: 1442 RQGVESVAMLPLFLVAKLGLCSGFPLADLEMRPHGISTFDKLCALSCRLTDDIHDFAEEK 1621
+ G+ S L L+ L + P D+ + S +L ++ RL DDI DF +EK
Sbjct: 501 KNGLASSGSCVLNLIPLLLMGQILP-EDILEQIFFPSKIHELLEMTIRLKDDITDFEQEK 559
Query: 1622 EQGFNISAVESYAKDNPECTLEEVVAFLRDMTQGFMKNIVQHYFSTHTAPEQSQTPEDSF 1801
E+G S++E Y KDNPE T E+ + ++ + + + + P
Sbjct: 560 ERGEIASSLECYLKDNPESTREDALNHIKGILDLSVSELNWEFLKHDNVP---------L 610
Query: 1802 YFKRTTLEMTRTFNFFYSYGDGFGHLNDGLINQIDAILFQPI 1927
K+ T R +F + Y DG ++ + +QI +L +P+
Sbjct: 611 CCKKFTFNFARGMHFLFKYKDGVSISDNEVKDQILKVLIEPV 652
Score = 83.6 bits (205), Expect(3) = 5e-68
Identities = 53/135 (39%), Positives = 83/135 (61%), Gaps = 3/135 (2%)
Frame = +2
Query: 71 GSFRNPKVDENQKISWNDSFIQSITSPFSDPLQGEKADNLTKKIKWGIM-EAFDKCG-AE 244
G+ +V + W+D I S+++ + P ++ + L + IK ++ E D C ++
Sbjct: 77 GNDAERRVANHHSNLWDDDVIHSLSTSYEAPAYRQRVETLIEDIKRLLLIEMEDSCSDSD 136
Query: 245 NDLIERLSLIDCLLCLGIDRLFQEEIRRDLDYVYMKAWNSSSGIGDGTK-SPKANIVSTA 421
+DLI+RL ++D + CLGIDR FQ EI+ +DYVY + WN GIG G++ S ++ STA
Sbjct: 137 DDLIKRLQIVDTIECLGIDRHFQPEIKVAVDYVY-RCWN-ERGIGLGSRDSLIKDLNSTA 194
Query: 422 LAFKLLRLHRYQNVS 466
L F+ LR+HRY NVS
Sbjct: 195 LGFQALRMHRY-NVS 208
Score = 67.4 bits (163), Expect(3) = 5e-68
Identities = 34/91 (37%), Positives = 53/91 (58%)
Frame = +3
Query: 540 VAKALNLVRASYLQFHGETILEEAKTSCTSFLNSFISLQQNVTESKDDFARLKQEVEHTL 719
V L+L RAS + F GE +++EAK T +LN ++ Q VT+ R EV++ L
Sbjct: 240 VRSMLSLFRASNISFPGEKVMDEAKIFTTEYLNQVLT-GQAVTDVDQSLLR---EVKYAL 295
Query: 720 QYQWILTTPRWEARRFIDFYSANKDWLRYSL 812
++ W + PRWEAR +I+ Y N WL+ ++
Sbjct: 296 EFPWHCSVPRWEARNYIEIYEQNYSWLKSNI 326
>gb|ADZ45513.1| alpha-humulene synthase [Picea glauca]
Length = 575
Score = 157 bits (397), Expect(3) = 3e-66
Identities = 106/344 (30%), Positives = 169/344 (49%), Gaps = 5/344 (1%)
Frame = +2
Query: 911 NLNMKILQLAKLDYNIVQAKLQKDLKEVSRWWNNTEISKLRNFRDGLVRSFLIPACGIYE 1090
N+N ++L+LAKLD++I+Q QK+++ ++RWW ++EI++L +R V + I+E
Sbjct: 245 NINQEVLELAKLDFSILQCIHQKEIQCITRWWRDSEIAQLNFYRRRHVELYFWAVTCIFE 304
Query: 1091 PELSGVRILTTKGCLGSYILDDVYDEFASQEELPIIIKAMEKLKLDANNGYCLKEHIITG 1270
PE S RI K +LDD+YD + +EL I +A+ + L + L +I
Sbjct: 305 PEFSPSRIAFAKITTVGAVLDDLYDTHGTLDELKTITEAVRRWDLSLIDD--LPNNIKIA 362
Query: 1271 CQFYLDANIQIITELNNIQGADTQKYLHQRIMGWAHASEQEAIWKFQGYVPTFEEYMRQG 1450
CQF+ + ++ E+ QG D L + + QEA W +VP+F EY++
Sbjct: 363 CQFFFNTANELAVEVVKKQGRDMTALLKATWQRYVESYLQEAEWIETRHVPSFNEYIKNA 422
Query: 1451 VES-----VAMLPLFLVAKLGLCSGFPLADLEMRPHGISTFDKLCALSCRLTDDIHDFAE 1615
+ S V ++PL L LG + + + P S +L L+ RL DDI DF +
Sbjct: 423 LVSSGMCIVNLIPLLL---LGQLLANNIVEQILSP---SKIQELSELTIRLIDDIRDFED 476
Query: 1616 EKEQGFNISAVESYAKDNPECTLEEVVAFLRDMTQGFMKNIVQHYFSTHTAPEQSQTPED 1795
EKE+G S VE Y KDNP+ TLE + ++ + ++ + + + P
Sbjct: 477 EKERGEIASIVECYMKDNPDSTLENALNHIKGILHVSLEELNWEFMKDDSVP-------- 528
Query: 1796 SFYFKRTTLEMTRTFNFFYSYGDGFGHLNDGLINQIDAILFQPI 1927
K+ T + R F Y YGDG N + +QI IL I
Sbjct: 529 -LCCKKFTFNIVRGLQFLYKYGDGISISNKEVKDQIFKILVDQI 571
Score = 77.8 bits (190), Expect(3) = 3e-66
Identities = 50/123 (40%), Positives = 71/123 (57%), Gaps = 2/123 (1%)
Frame = +2
Query: 116 WNDSFIQSITSPFSDPLQGEKADNLTKKIKWGIMEAFDKCGAENDLIERLSLIDCLLCLG 295
W+D I S+ SP+ P E+ L +++K ++ + + +DLI RL ++D L CLG
Sbjct: 22 WDDDLILSLDSPYGAPAYYERLAKLIEEMKHLLLREMED--SNHDLIRRLQIVDTLECLG 79
Query: 296 IDRLFQEEIR-RDLDYVYMKAWNSSSGIGDGTK-SPKANIVSTALAFKLLRLHRYQNVSP 469
IDR FQ EI+ L YVY + WN GIG G+ S ++ +TAL + LRLHRY S
Sbjct: 80 IDRHFQHEIKTAALHYVY-RCWN-EKGIGMGSSDSGSKDLDATALGLRALRLHRYNVSSG 137
Query: 470 VSE 478
V E
Sbjct: 138 VLE 140
Score = 67.8 bits (164), Expect(3) = 3e-66
Identities = 31/91 (34%), Positives = 53/91 (58%)
Frame = +3
Query: 540 VAKALNLVRASYLQFHGETILEEAKTSCTSFLNSFISLQQNVTESKDDFARLKQEVEHTL 719
V L+L+RAS + F GE +++EAK +L ++ +VT+ R EV++ L
Sbjct: 159 VRSMLSLLRASEISFPGEKVMQEAKAFTREYLTQVLAGSGDVTDVDQSLLR---EVKYAL 215
Query: 720 QYQWILTTPRWEARRFIDFYSANKDWLRYSL 812
++ W + PRWEA+ FI+ Y N+ WL+ ++
Sbjct: 216 EFPWYCSAPRWEAKSFIEIYGQNQSWLKSNI 246
>gb|ABW80964.1| alpha pinene synthase [Chamaecyparis formosensis]
Length = 628
Score = 125 bits (314), Expect(3) = 6e-59
Identities = 94/317 (29%), Positives = 146/317 (46%), Gaps = 1/317 (0%)
Frame = +2
Query: 923 KILQLAKLDYNIVQAKLQKDLKEVSRWWNNTEISKLRNFRDGLVRSFLIPACGIYEPELS 1102
K+L+LAKLD+NI+Q+ Q++L+ +SRWW +++ KL R V +L A G EP+ S
Sbjct: 305 KLLELAKLDFNIIQSLQQQELQILSRWWMESDLGKLDFARHRHVEYYLWAATGCIEPKYS 364
Query: 1103 GVRILTTKGCLGSYILDDVYDEFASQEELPIIIKAMEKLKLDANNGYCLKEHIITGCQFY 1282
RI K LDD+YD + +E+ I KA+++ G L E + + +
Sbjct: 365 AFRIGFAKLSALVTYLDDMYDTY-DFDEIKIFTKAIKRWDASIIKG--LPEFMKVAFKAF 421
Query: 1283 LDANIQIITELNNIQGADTQKYLHQRIMGWAHASEQEAIWKFQGYVPTFEEYMRQGVESV 1462
+A + E QG DT Y + + A +EA W GY+P+ EEY+ G S
Sbjct: 422 DEAVKDMAQEAKKTQGRDTLDYARKAWEVYIDAYMKEAEWLATGYMPSLEEYLENGKVSA 481
Query: 1463 AMLPLFLVAKLGLCSGFPLADLEMRPHGI-STFDKLCALSCRLTDDIHDFAEEKEQGFNI 1639
+ L L L PL+D ++ S FD+L L+ RL D F E ++G +
Sbjct: 482 GSRVVTLQPILSL--DVPLSDDILKEIDYPSRFDELLCLTLRLRGDTRTFKAEADRGEVV 539
Query: 1640 SAVESYAKDNPECTLEEVVAFLRDMTQGFMKNIVQHYFSTHTAPEQSQTPEDSFYFKRTT 1819
S + Y KD+P E+ + +L + +K + Y T P S K
Sbjct: 540 SCITCYMKDHPGSNEEDALNYLNSLIDERLKELNWEYLKTDNVPIIS---------KGNA 590
Query: 1820 LEMTRTFNFFYSYGDGF 1870
+++ Y DGF
Sbjct: 591 YNLSKGLQLLYKERDGF 607
Score = 94.0 bits (232), Expect(3) = 6e-59
Identities = 53/122 (43%), Positives = 77/122 (63%)
Frame = +2
Query: 116 WNDSFIQSITSPFSDPLQGEKADNLTKKIKWGIMEAFDKCGAENDLIERLSLIDCLLCLG 295
W+D FIQS++ + P GE+A+ L K ++ + +DLI+ LSL+D + LG
Sbjct: 77 WDDDFIQSLSKAYEAPSYGERAEKLIKDVRDMFNALPLHSSSADDLIQHLSLVDSVERLG 136
Query: 296 IDRLFQEEIRRDLDYVYMKAWNSSSGIGDGTKSPKANIVSTALAFKLLRLHRYQNVSPVS 475
IDR FQ EI+ LDYVY + W S +GIG G +S A++ +TAL F++LRLHRY S V
Sbjct: 137 IDRHFQNEIKTALDYVY-RYW-SDAGIGCGRESTHADLNTTALGFRILRLHRYSVSSDVL 194
Query: 476 ER 481
++
Sbjct: 195 QQ 196
Score = 58.9 bits (141), Expect(3) = 6e-59
Identities = 33/108 (30%), Positives = 53/108 (49%)
Frame = +3
Query: 540 VAKALNLVRASYLQFHGETILEEAKTSCTSFLNSFISLQQNVTESKDDFARLKQEVEHTL 719
+ LNL R S + F GE +L++AK+ ++L + N+ L +E++ L
Sbjct: 216 IKNILNLFRGSLIAFPGENVLDDAKSFTMTYLKQVLPKISNLN--------LSREIKFNL 267
Query: 720 QYQWILTTPRWEARRFIDFYSANKDWLRYSLFLFFNIFHVNLSNVFKV 863
+Y W PR EAR +ID Y + W S+ NIF+ L + K+
Sbjct: 268 EYGWHTNVPRLEARTYIDIYGEDSSWASKSI---NNIFYTKLLELAKL 312
>gb|ADX42738.1| (E)-beta-farnesene synthase 2 [Pseudotsuga menziesii]
gi|323364474|gb|ADX42739.1| (E)-beta-farnesene synthase 2
[Pseudotsuga menziesii]
Length = 825
Score = 147 bits (370), Expect(3) = 1e-58
Identities = 101/340 (29%), Positives = 170/340 (50%), Gaps = 3/340 (0%)
Frame = +2
Query: 917 NMKILQLAKLDYNIVQAKLQKDLKEVSRWWNNTEISKLRNFRDGLVRSFLIPACGIYEPE 1096
N KIL+LAKLD+NI+QA QK++K V+ W+ N+E L R+ V F I A G YEP+
Sbjct: 492 NEKILELAKLDFNIIQAIHQKEMKNVTTWFKNSEFPLLPFGRERPVECFFIVAAGTYEPQ 551
Query: 1097 LSGVRILTTKGCLGSYILDDVYDEFASQEELPIIIKAMEKLKLDANNGYCLKEHIITGCQ 1276
+ R L +K + +LDD+YD + + +EL + +A+ + L L +++ +
Sbjct: 552 YAKCRFLFSKVACLNTVLDDMYDTYGTLDELKLFTEAVRRWDLSLTEN--LPDYMKLCYK 609
Query: 1277 FYLDANIQIITELNNIQGADTQKYLHQRIMGWAHASEQEAIWKFQGYVPTFEEYMRQGVE 1456
+ D +++ E QG + + + + +EA W Y+P+ EEY+R G+
Sbjct: 610 IFYDIVHEVVLEAEKEQGRELLTFFRKGWEEYLMGYYEEAEWLACEYMPSLEEYIRNGII 669
Query: 1457 SVAMLPLFLVAKLGLCSGFPLAD--LEMRPH-GISTFDKLCALSCRLTDDIHDFAEEKEQ 1627
S+ + +V+ + L G L+ LE + G +L ++ RL DDIH + EK +
Sbjct: 670 SIGQ-RILVVSGVLLMEGQILSQEALEQLDYPGRRVLTELNSIITRLADDIHTYKAEKAR 728
Query: 1628 GFNISAVESYAKDNPECTLEEVVAFLRDMTQGFMKNIVQHYFSTHTAPEQSQTPEDSFYF 1807
G S++E Y K++P T E V ++ + + +K + + PE S T F
Sbjct: 729 GELASSIECYMKEHPGSTEEVAVNYMYSLLEPAVKELTWEFLK----PEDS-TVHIPFQC 783
Query: 1808 KRTTLEMTRTFNFFYSYGDGFGHLNDGLINQIDAILFQPI 1927
K+ +E TR + GDGFG + + I L +P+
Sbjct: 784 KKMLMEETRVTMVIFKEGDGFGISKTKIKDYIKDCLIEPL 823
Score = 73.9 bits (180), Expect(3) = 1e-58
Identities = 44/82 (53%), Positives = 57/82 (69%)
Frame = +2
Query: 224 FDKCGAENDLIERLSLIDCLLCLGIDRLFQEEIRRDLDYVYMKAWNSSSGIGDGTKSPKA 403
F C DL+ERL ++D L+ LGIDR F++EI+ LDYV+ + WN GIG G +P A
Sbjct: 293 FVPCLYPVDLLERLLIVDNLVQLGIDRHFEKEIKEALDYVH-RHWN-ERGIGWGRLNPIA 350
Query: 404 NIVSTALAFKLLRLHRYQNVSP 469
++ TAL F+LLRLHRY NVSP
Sbjct: 351 DLEITALGFRLLRLHRY-NVSP 371
Score = 56.2 bits (134), Expect(3) = 1e-58
Identities = 33/115 (28%), Positives = 63/115 (54%)
Frame = +3
Query: 471 FQNGSGHGFLEEEISSSLFTRTSVAKALNLVRASYLQFHGETILEEAKTSCTSFLNSFIS 650
F++ +GH + S+ VA L+L RAS L F GE IL+EAK+ + +L +
Sbjct: 377 FKDSNGHF-----VCSAAQFNKDVASMLSLYRASQLAFPGENILDEAKSFTSKYLKEALE 431
Query: 651 LQQNVTESKDDFARLKQEVEHTLQYQWILTTPRWEARRFIDFYSANKDWLRYSLF 815
++ + + ++ L +E+++ L+ W + PR EA+R+ Y ++ +L S++
Sbjct: 432 KRETYS-AWNNKQSLSEEIKYALENSWHASVPRVEAKRYCQVYRSDYTYLAKSVY 485
>gb|ADX42737.1| (E)-beta-farnesene synthase 1 [Pseudotsuga menziesii]
Length = 825
Score = 147 bits (370), Expect(3) = 1e-58
Identities = 101/340 (29%), Positives = 170/340 (50%), Gaps = 3/340 (0%)
Frame = +2
Query: 917 NMKILQLAKLDYNIVQAKLQKDLKEVSRWWNNTEISKLRNFRDGLVRSFLIPACGIYEPE 1096
N KIL+LAKLD+NI+QA QK++K V+ W+ N+E L R+ V F I A G YEP+
Sbjct: 492 NEKILELAKLDFNIIQAIHQKEMKNVTTWFKNSEFPLLPFGRERPVECFFIVAAGTYEPQ 551
Query: 1097 LSGVRILTTKGCLGSYILDDVYDEFASQEELPIIIKAMEKLKLDANNGYCLKEHIITGCQ 1276
+ R L +K + +LDD+YD + + +EL + +A+ + L L +++ +
Sbjct: 552 YAKCRFLFSKVACLNTVLDDMYDTYGTLDELKLFTEAVRRWDLSLTEN--LPDYMKLCYK 609
Query: 1277 FYLDANIQIITELNNIQGADTQKYLHQRIMGWAHASEQEAIWKFQGYVPTFEEYMRQGVE 1456
+ D +++ E QG + + + + +EA W Y+P+ EEY+R G+
Sbjct: 610 IFYDIVHEVVLEAEKEQGRELLTFFRKGWEEYLMGYYEEAEWLACEYMPSLEEYIRNGII 669
Query: 1457 SVAMLPLFLVAKLGLCSGFPLAD--LEMRPH-GISTFDKLCALSCRLTDDIHDFAEEKEQ 1627
S+ + +V+ + L G L+ LE + G +L ++ RL DDIH + EK +
Sbjct: 670 SIGQ-RILVVSGVLLMEGQILSQEALEQLDYPGRRVLTELNSIITRLADDIHTYKAEKAR 728
Query: 1628 GFNISAVESYAKDNPECTLEEVVAFLRDMTQGFMKNIVQHYFSTHTAPEQSQTPEDSFYF 1807
G S++E Y K++P T E V ++ + + +K + + PE S T F
Sbjct: 729 GELASSIECYMKEHPGSTEEVAVNYMYSLLEPAVKELTWEFLK----PEDS-TVHIPFQC 783
Query: 1808 KRTTLEMTRTFNFFYSYGDGFGHLNDGLINQIDAILFQPI 1927
K+ +E TR + GDGFG + + I L +P+
Sbjct: 784 KKMLMEETRVTMVIFKEGDGFGISKTKIKDYIKDCLIEPL 823
Score = 73.9 bits (180), Expect(3) = 1e-58
Identities = 44/82 (53%), Positives = 57/82 (69%)
Frame = +2
Query: 224 FDKCGAENDLIERLSLIDCLLCLGIDRLFQEEIRRDLDYVYMKAWNSSSGIGDGTKSPKA 403
F C DL+ERL ++D L+ LGIDR F++EI+ LDYV+ + WN GIG G +P A
Sbjct: 293 FVPCLYPVDLLERLLIVDNLVQLGIDRHFEKEIKEALDYVH-RHWN-ERGIGWGRLNPIA 350
Query: 404 NIVSTALAFKLLRLHRYQNVSP 469
++ TAL F+LLRLHRY NVSP
Sbjct: 351 DLEITALGFRLLRLHRY-NVSP 371
Score = 56.2 bits (134), Expect(3) = 1e-58
Identities = 33/115 (28%), Positives = 63/115 (54%)
Frame = +3
Query: 471 FQNGSGHGFLEEEISSSLFTRTSVAKALNLVRASYLQFHGETILEEAKTSCTSFLNSFIS 650
F++ +GH + S+ VA L+L RAS L F GE IL+EAK+ + +L +
Sbjct: 377 FKDSNGHF-----VCSAAQFNKDVASMLSLYRASQLAFPGENILDEAKSFTSKYLKEALE 431
Query: 651 LQQNVTESKDDFARLKQEVEHTLQYQWILTTPRWEARRFIDFYSANKDWLRYSLF 815
++ + + ++ L +E+++ L+ W + PR EA+R+ Y ++ +L S++
Sbjct: 432 KRETYS-AWNNKQSLSEEIKYALENSWHASVPRVEAKRYCQVYRSDYTYLAKSVY 485
>sp|O64405.1|TPSD5_ABIGR RecName: Full=Gamma-humulene synthase; AltName: Full=Agfghum
gi|2961462|gb|AAC05728.1| gamma-humulene synthase [Abies
grandis]
Length = 593
Score = 225 bits (573), Expect = 7e-56
Identities = 179/608 (29%), Positives = 294/608 (48%), Gaps = 4/608 (0%)
Frame = +2
Query: 116 WNDSFIQSITSPFSDPLQGEKADNLTKKIKWGIMEAFD-KCG-AENDLIERLSLIDCLLC 289
W D IQS+ SP+ P E+++ L ++IK + D C ++ DLI+RL ++D + C
Sbjct: 30 WEDDRIQSLNSPYGAPAYQERSEKLIEEIKLLFLSDMDDSCNDSDRDLIKRLEIVDTVEC 89
Query: 290 LGIDRLFQEEIRRDLDYVYMKAWNSSSGIGDGTK-SPKANIVSTALAFKLLRLHRYQNVS 466
LGIDR FQ EI+ LDYVY + WN GIG+G++ S K ++ +TAL F+ LRLHRY S
Sbjct: 90 LGIDRHFQPEIKLALDYVY-RCWNER-GIGEGSRDSLKKDLNATALGFRALRLHRYNVSS 147
Query: 467 PVSERQRTWFSRRGNKLFLVY*DISSKGFEFGESFISPIPWRNYIGRSQDIVYKFSKFIY 646
V E R N F + +G E++ + + R+ +I++ K
Sbjct: 148 GVLENFRD-----DNGQFFCGSTVEEEG---AEAYNKHVRCMLSLSRASNILFPGEK--- 196
Query: 647 LVTAERYRIKR*FCPIKARGRAYITISMDSHHSKMGGKKVY*FLFSKQGLAKVFLIFIF* 826
+ +A+ T + + G++ S G K L F +
Sbjct: 197 ---------------VMEEAKAFTTNYLKK---VLAGREATHVDESLLGEVKYALEFPW- 237
Query: 827 YFSCEFIQCLQSTGTIRLCYFMYLHNSHNLNMKILQLAKLDYNIVQAKLQKDLKEVSRWW 1006
C +Q ++ I + + NL+ K+L+LAKLD+NI+Q QK+L+ +SRW+
Sbjct: 238 --HCS-VQRWEARSFIEIFGQIDSELKSNLSKKMLELAKLDFNILQCTHQKELQIISRWF 294
Query: 1007 NNTEISKLRNFRDGLVRSFLIPACGIYEPELSGVRILTTKGCLGSYILDDVYDEFASQEE 1186
++ I+ L +R V + A I EPE SG R+ TK + +LDD+YD + ++
Sbjct: 295 ADSSIASLNFYRKCYVEFYFWMAAAISEPEFSGSRVAFTKIAILMTMLDDLYDTHGTLDQ 354
Query: 1187 LPIIIKAMEKLKLDANNGYCLKEHIITGCQFYLDANIQIITELNNIQGADTQKYLHQRIM 1366
L I + + + + G L + + +F+L + ++I E QG D Y+ +
Sbjct: 355 LKIFTEGVRRWDVSLVEG--LPDFMKIAFEFWLKTSNELIAEAVKAQGQDMAAYIRKNAW 412
Query: 1367 -GWAHASEQEAIWKFQGYVPTFEEYMRQGVESVAMLPLFLVAKLGLCSGFPLADLEMRPH 1543
+ A Q+A W G+VPTF+EY+ G + M L L+ L + P+ LE +
Sbjct: 413 ERYLEAYLQDAEWIATGHVPTFDEYLNNGTPNTGMCVLNLIPLLLMGEHLPIDILE-QIF 471
Query: 1544 GISTFDKLCALSCRLTDDIHDFAEEKEQGFNISAVESYAKDNPECTLEEVVAFLRDMTQG 1723
S F L L+ RL DD DF EK+ G ++S +E Y KD+PE T+E+ + + +
Sbjct: 472 LPSRFHHLIELASRLVDDARDFQAEKDHG-DLSCIECYLKDHPESTVEDALNHVNGLLGN 530
Query: 1724 FMKNIVQHYFSTHTAPEQSQTPEDSFYFKRTTLEMTRTFNFFYSYGDGFGHLNDGLINQI 1903
+ + + + S + SF+ + R+ F Y+ GDGF N + +Q+
Sbjct: 531 CLLEMNWKFLKKQDSVPLS-CKKYSFHV------LARSIQFMYNQGDGFSISNKVIKDQV 583
Query: 1904 DAILFQPI 1927
+L P+
Sbjct: 584 QKVLIVPV 591
>gb|ADH29869.1| e-beta farnesene synthase [Pinus sylvestris]
Length = 811
Score = 149 bits (376), Expect(3) = 3e-55
Identities = 97/340 (28%), Positives = 170/340 (50%), Gaps = 3/340 (0%)
Frame = +2
Query: 917 NMKILQLAKLDYNIVQAKLQKDLKEVSRWWNNTEISKLRNFRDGLVRSFLIPACGIYEPE 1096
N KIL+LAKLD+NI+QA QK++K V+ W+ ++E L R+ V F + A G YEP+
Sbjct: 477 NQKILELAKLDFNIIQAIHQKEMKNVTSWFKHSEFPLLPFGRERPVECFFLVAAGTYEPQ 536
Query: 1097 LSGVRILTTKGCLGSYILDDVYDEFASQEELPIIIKAMEKLKLDANNGYCLKEHIITGCQ 1276
+ R L +K + +LDD+YD + + +EL + +A+ + L L +++ +
Sbjct: 537 YAKCRFLFSKVACLNTVLDDMYDTYGTLDELKLFTEAVRRWDLSLTES--LPDYMKLCYK 594
Query: 1277 FYLDANIQIITELNNIQGADTQKYLHQRIMGWAHASEQEAIWKFQGYVPTFEEYMRQGVE 1456
+ + +++ E +QG + + + + +EA W YVP+ EEY+R G+
Sbjct: 595 IFYEIVHEVVREAEKLQGRELLSFFRKGWEEYLLGYYEEAEWLASEYVPSLEEYIRNGII 654
Query: 1457 SVAMLPLFLVAKLGLCSGFPLAD--LEMRPH-GISTFDKLCALSCRLTDDIHDFAEEKEQ 1627
S+ + LV+ + L G L+ LE + G +L + RL DDIH + EK +
Sbjct: 655 SIGQ-RILLVSGVLLMEGQILSQEALEKLDYPGRRVLTELNCIITRLADDIHTYKAEKAR 713
Query: 1628 GFNISAVESYAKDNPECTLEEVVAFLRDMTQGFMKNIVQHYFSTHTAPEQSQTPEDSFYF 1807
G S++E Y K++P T E V ++ + + +K + + P+ + + F
Sbjct: 714 GELASSIECYMKEHPGSTEEVAVNYMYSLLEPAVKELTWEFLK----PDDTSDVDIPFQC 769
Query: 1808 KRTTLEMTRTFNFFYSYGDGFGHLNDGLINQIDAILFQPI 1927
K+ +E TR + GDGFG + + I L +P+
Sbjct: 770 KKMLMEETRVTMVIFKEGDGFGISKTKIKDYIKECLIEPL 809
Score = 58.9 bits (141), Expect(3) = 3e-55
Identities = 35/77 (45%), Positives = 48/77 (62%)
Frame = +2
Query: 248 DLIERLSLIDCLLCLGIDRLFQEEIRRDLDYVYMKAWNSSSGIGDGTKSPKANIVSTALA 427
DL+E L ++D ++ LGIDR F++EI+ LD VY + WN S +P A++ TAL
Sbjct: 292 DLLEGLLMVDNIVRLGIDRHFEKEIKEILDCVY-RHWNES-------LNPVADLEITALG 343
Query: 428 FKLLRLHRYQNVSPVSE 478
F+LLRLHRY V E
Sbjct: 344 FRLLRLHRYSVTPAVFE 360
Score = 57.8 bits (138), Expect(3) = 3e-55
Identities = 38/115 (33%), Positives = 64/115 (55%)
Frame = +3
Query: 471 FQNGSGHGFLEEEISSSLFTRTSVAKALNLVRASYLQFHGETILEEAKTSCTSFLNSFIS 650
F++ GH F S+S F + +VA L+L RAS L F GETIL+EA+ T +L +
Sbjct: 362 FKDADGHFFG----STSQFNK-NVASMLSLYRASQLAFPGETILDEARDFATKYLREALE 416
Query: 651 LQQNVTESKDDFARLKQEVEHTLQYQWILTTPRWEARRFIDFYSANKDWLRYSLF 815
+ + + ++ L QE+++ L+ W + R EA+R+ YS++ L S++
Sbjct: 417 -KSEIFTAWNNKQNLSQEIQYELENSWHASVSRVEAKRYCQGYSSDYARLAKSVY 470
>dbj|BAF43701.1| putative sesquiterpene synthase [Cycas taitungensis]
Length = 846
Score = 137 bits (345), Expect(3) = 4e-54
Identities = 91/338 (26%), Positives = 162/338 (47%), Gaps = 1/338 (0%)
Frame = +2
Query: 917 NMKILQLAKLDYNIVQAKLQKDLKEVSRWWNNTEISKLRNFRDGLVRSFLIPACGIYEPE 1096
N K L+LAKLD+NI+Q+ Q+++K+++RW+ ++ + + R + + G +EPE
Sbjct: 520 NNKYLELAKLDFNILQSVHQQEMKDLTRWFGDSGLRQFPFARQRPIELYFNVCMGTFEPE 579
Query: 1097 LSGVRILTTK-GCLGSYILDDVYDEFASQEELPIIIKAMEKLKLDANNGYCLKEHIITGC 1273
R + K GCLG I+DD++D + + +EL + +A+E+ L + L E++
Sbjct: 580 FYRCRFIFAKVGCLG-LIVDDLHDTYGTLDELILFTEAVERWDLSKTDS--LPEYMKIWF 636
Query: 1274 QFYLDANIQIITELNNIQGADTQKYLHQRIMGWAHASEQEAIWKFQGYVPTFEEYMRQGV 1453
+ + D ++ E QG + + G+ + +EA W YVPT + Y++ G+
Sbjct: 637 RVWYDIVDELAREAEEEQGHELMGIFRRVWEGYIGCNLEEAQWLAADYVPTMDAYIKNGI 696
Query: 1454 ESVAMLPLFLVAKLGLCSGFPLADLEMRPHGISTFDKLCALSCRLTDDIHDFAEEKEQGF 1633
SV +P+ L+ + ++ RP S +L L CR TDD F EK +G
Sbjct: 697 TSVG-VPIILMYGIFFMGQLLPENILDRPDNPSKIIELVGLICRFTDDAKTFQAEKARGE 755
Query: 1634 NISAVESYAKDNPECTLEEVVAFLRDMTQGFMKNIVQHYFSTHTAPEQSQTPEDSFYFKR 1813
S++E Y K+N T ++ + +R T +K + Y P + K+
Sbjct: 756 LASSIECYMKENSGSTQDDALNHIRAATSSAVKEMTWEYLKPDDVP---------YRCKK 806
Query: 1814 TTLEMTRTFNFFYSYGDGFGHLNDGLINQIDAILFQPI 1927
E+ R + Y GD +G N + + I L +P+
Sbjct: 807 FVFEVARVWMLMYKDGDPYGISNKEMQDYIREALIEPV 844
Score = 73.9 bits (180), Expect(3) = 4e-54
Identities = 39/78 (50%), Positives = 52/78 (66%)
Frame = +2
Query: 248 DLIERLSLIDCLLCLGIDRLFQEEIRRDLDYVYMKAWNSSSGIGDGTKSPKANIVSTALA 427
DL+ERL +D + LGIDR F+ +I+ LDYVY + WN SGI G +S ++ +TAL
Sbjct: 326 DLLERLLAVDTVERLGIDRHFERQIKESLDYVY-RHWNDESGIACGRESVLPDLETTALG 384
Query: 428 FKLLRLHRYQNVSPVSER 481
F+LLRLHRY S V E+
Sbjct: 385 FRLLRLHRYNVSSAVFEK 402
Score = 50.8 bits (120), Expect(3) = 4e-54
Identities = 28/93 (30%), Positives = 48/93 (51%)
Frame = +3
Query: 540 VAKALNLVRASYLQFHGETILEEAKTSCTSFLNSFISLQQNVTESKDDFARLKQEVEHTL 719
V L+L RAS + F GE +L+EAK+ T++L + ++D L EV++ L
Sbjct: 423 VESMLSLYRASQIAFPGEDVLDEAKSFTTTYLTKALQ-NSEACRMRNDKQNLGHEVKYAL 481
Query: 720 QYQWILTTPRWEARRFIDFYSANKDWLRYSLFL 818
+ W + PR E +R+ Y + L S+++
Sbjct: 482 ENPWHSSVPRLETKRYCQLYPPDHARLGKSVYM 514
>gb|ABV44452.1| caryophyllene/humulene synthase [Pinus sylvestris]
Length = 575
Score = 167 bits (424), Expect(2) = 1e-53
Identities = 102/339 (30%), Positives = 172/339 (50%)
Frame = +2
Query: 911 NLNMKILQLAKLDYNIVQAKLQKDLKEVSRWWNNTEISKLRNFRDGLVRSFLIPACGIYE 1090
N N K+L+LAKLD+NI+Q QK+++ ++RWW ++E++++ +R + + I+E
Sbjct: 245 NFNQKVLELAKLDFNILQCIHQKEMQYITRWWRDSEVAQVNFYRRRHMELYFWAVISIFE 304
Query: 1091 PELSGVRILTTKGCLGSYILDDVYDEFASQEELPIIIKAMEKLKLDANNGYCLKEHIITG 1270
PE S RI K +LDD+YD + +EL I + + + + + L E I
Sbjct: 305 PEFSQSRIAFAKVTTVGTVLDDLYDTYGMLDELKTITEGVRRWDISLIDD--LPEKIKIA 362
Query: 1271 CQFYLDANIQIITELNNIQGADTQKYLHQRIMGWAHASEQEAIWKFQGYVPTFEEYMRQG 1450
QF+ + ++ E+ + QG DT L + + + QEA W G VPTF EY++
Sbjct: 363 IQFFFNTANELAAEVVSKQGPDTSAILKDTWVRYLESYLQEAEWITTGCVPTFSEYIKNA 422
Query: 1451 VESVAMLPLFLVAKLGLCSGFPLADLEMRPHGISTFDKLCALSCRLTDDIHDFAEEKEQG 1630
V S M + L+ L + P ++ + H S +L L+ RL DD+ DF +EKE+G
Sbjct: 423 VASSGMCIVNLIPLLLMGQPLP-NNILQQIHSPSKIQELSELTIRLIDDLRDFEDEKERG 481
Query: 1631 FNISAVESYAKDNPECTLEEVVAFLRDMTQGFMKNIVQHYFSTHTAPEQSQTPEDSFYFK 1810
S +E Y KDNP+ T+ + ++ + Q ++ + + + + P K
Sbjct: 482 EMASIIECYIKDNPDSTVGNALNRIKGILQLSLEEMNREFLKQDSVP---------LCCK 532
Query: 1811 RTTLEMTRTFNFFYSYGDGFGHLNDGLINQIDAILFQPI 1927
+ T +TR F Y YGDG N+ + +QI IL + +
Sbjct: 533 KFTFNITRGLQFLYKYGDGISISNNEVKDQIFKILVEQV 571
Score = 72.0 bits (175), Expect(2) = 1e-53
Identities = 39/120 (32%), Positives = 64/120 (53%)
Frame = +3
Query: 444 SIDIKMSLQFQNGSGHGFLEEEISSSLFTRTSVAKALNLVRASYLQFHGETILEEAKTSC 623
++D + F++G+G F + V L+L+RAS + F GE ++EEAK
Sbjct: 133 NVDSGVLENFKDGNGKFFC------NFSGDKEVRSMLSLLRASEISFPGEKVMEEAKAYT 186
Query: 624 TSFLNSFISLQQNVTESKDDFARLKQEVEHTLQYQWILTTPRWEARRFIDFYSANKDWLR 803
+LN ++ + VT L++EV++ L++ W + PRWEAR FI+ Y N WL+
Sbjct: 187 REYLNQVLAARGEVTGVDQS---LREEVKYALEFPWHCSAPRWEARSFIEIYGENHSWLK 243
Score = 78.6 bits (192), Expect = 1e-11
Identities = 50/123 (40%), Positives = 71/123 (57%), Gaps = 2/123 (1%)
Frame = +2
Query: 116 WNDSFIQSITSPFSDPLQGEKADNLTKKIKWGIMEAFDKCGAENDLIERLSLIDCLLCLG 295
W+D FI S+ SP+ P E+ L ++IK ++ + + D I+RL ++D CLG
Sbjct: 22 WDDEFIVSLNSPYGAPAYYERVGKLIEEIKHLLLSEMED--SNYDFIKRLRIVDTFECLG 79
Query: 296 IDRLFQEEIRR-DLDYVYMKAWNSSSGIGDGTKS-PKANIVSTALAFKLLRLHRYQNVSP 469
IDR FQ EI+ LDYVY + WN GI G++ ++ +TAL F+ LRLHRY S
Sbjct: 80 IDRHFQHEIKTVALDYVY-RYWN-EKGIAVGSRDFLNRDLNTTALGFRALRLHRYNVDSG 137
Query: 470 VSE 478
V E
Sbjct: 138 VLE 140
>gb|AAS47689.1| E-alpha-bisabolene synthase [Picea abies]
Length = 807
Score = 132 bits (331), Expect(3) = 3e-53
Identities = 91/340 (26%), Positives = 158/340 (46%), Gaps = 3/340 (0%)
Frame = +2
Query: 917 NMKILQLAKLDYNIVQAKLQKDLKEVSRWWNNTEISKLRNFRDGLVRSFLIPACGIYEPE 1096
N KIL+LAKLD+NI+Q+ QK++K V+ W+ ++ + R+ + + + A G YEP+
Sbjct: 478 NEKILELAKLDFNIIQSIHQKEMKNVTSWFRDSGLPLFTFARERPLEFYFLIAGGTYEPQ 537
Query: 1097 LSGVRILTTKGCLGSYILDDVYDEFASQEELPIIIKAMEKLKLDANNGYCLKEHIITGCQ 1276
+ R L TK +LDD+YD + + EL + +A+ + L L +++ +
Sbjct: 538 YAKCRFLFTKVACLQTVLDDMYDTYGTPSELKLFTEAVRRWDLSFTEN--LPDYMKLCYK 595
Query: 1277 FYLDANIQIITELNNIQGADTQKYLHQRIMGWAHASEQEAIWKFQGYVPTFEEYMRQGVE 1456
Y D ++ E+ QG + + + + +EA W YVPT +EY++ G+
Sbjct: 596 IYYDIVHEVAWEVEKEQGRELVSFFRKGWEDYLLGYYEEAEWLAAEYVPTLDEYIKNGIT 655
Query: 1457 SVAMLPLFLVAKLGLCSGFPLADLEMRP---HGISTFDKLCALSCRLTDDIHDFAEEKEQ 1627
S+ L L L + G L+ + G +L +L RL DD + EK +
Sbjct: 656 SIGQRILLLSGVL-IMEGQLLSQEALEKVDYPGRRVLTELNSLISRLADDTKTYKAEKAR 714
Query: 1628 GFNISAVESYAKDNPECTLEEVVAFLRDMTQGFMKNIVQHYFSTHTAPEQSQTPEDSFYF 1807
G S++E Y KD+P C EE + + + + +K + + + P F
Sbjct: 715 GELASSIECYMKDHPGCQEEEALNHIYGILEPAVKELTREFLKADHVP---------FPC 765
Query: 1808 KRTTLEMTRTFNFFYSYGDGFGHLNDGLINQIDAILFQPI 1927
K+ + TR + GDGFG + + I L +P+
Sbjct: 766 KKMLFDETRVTMVIFKDGDGFGISKLEVKDHIKECLIEPL 805
Score = 76.6 bits (187), Expect(3) = 3e-53
Identities = 44/75 (58%), Positives = 56/75 (74%)
Frame = +2
Query: 248 DLIERLSLIDCLLCLGIDRLFQEEIRRDLDYVYMKAWNSSSGIGDGTKSPKANIVSTALA 427
DL+ERL ++D + LGIDR F++EI+ LDYVY + WN GIG G SP A++ +TAL
Sbjct: 287 DLLERLLIVDNVERLGIDRHFEKEIKEALDYVY-RHWN-DRGIGWGRLSPIADLETTALG 344
Query: 428 FKLLRLHRYQNVSPV 472
F+LLRLHRY NVSPV
Sbjct: 345 FRLLRLHRY-NVSPV 358
Score = 50.4 bits (119), Expect(3) = 3e-53
Identities = 30/92 (32%), Positives = 50/92 (54%)
Frame = +3
Query: 540 VAKALNLVRASYLQFHGETILEEAKTSCTSFLNSFISLQQNVTESKDDFARLKQEVEHTL 719
VA L+L RAS L F E+IL+EAK+ T +L + + + S + L +E+++ L
Sbjct: 381 VASMLSLYRASQLAFPEESILDEAKSFSTQYLREALEKSETFS-SWNHRQSLSEEIKYAL 439
Query: 720 QYQWILTTPRWEARRFIDFYSANKDWLRYSLF 815
+ W + PR EA+R+ Y + L S++
Sbjct: 440 KTSWHASVPRVEAKRYCQVYRQDYAHLAKSVY 471
>sp|H8ZM73.1|CAS_ABIBA RecName: Full=Bifunctional cis-abienol synthase, chloroplastic;
AltName: Full=Diterpene synthase TPS4; Short=AbdiTPS4;
Flags: Precursor gi|343174804|gb|AEL99953.1| diterpene
synthase TPS4 [Abies balsamea]
Length = 867
Score = 132 bits (331), Expect(3) = 1e-52
Identities = 95/338 (28%), Positives = 163/338 (48%), Gaps = 2/338 (0%)
Frame = +2
Query: 917 NMKILQLAKLDYNIVQAKLQKDLKEVSRWWNNTEISKLRNFRDGLVRSFLIPACGIYEPE 1096
N K L+LAKLD+N VQ+ Q++++E+ RW ++ ++L+ RD +V ++ A ++EPE
Sbjct: 544 NEKYLELAKLDFNSVQSLHQEEIRELVRWCKSSGFTELKFTRDRVVETYFAVASSMFEPE 603
Query: 1097 LSGVRILTTKGCLGSYILDDVYDEFASQEELPIIIKAMEKLKLDANNGYCLKEHI-ITGC 1273
S R + TK + ILDD+YD + S +E+ + +A+++ L + +H+ I
Sbjct: 604 FSTCRAVYTKISVLLVILDDLYDGYGSPDEIKLFSEAVKRWDLSLLEQ--MPDHMKICFL 661
Query: 1274 QFYLDANIQIITELNNIQGADTQKYLHQRIMGWAHASEQEAIWKFQGYVPTFEEYMRQGV 1453
Y N ++ E QG D Y+ A +EA W YVP+F+EY+
Sbjct: 662 GLYNTVN-EVAEEGRKTQGHDVLGYIRNLWEIQLAAFTREAEWSQGKYVPSFDEYIENAQ 720
Query: 1454 ESVAMLPLFLVAKLGLCSGFPLADLEMRPHGISTFDKLCALSCRLTDDIHDFAEEKEQGF 1633
S+ + + L+ L L+ ++ S F +L +L+ RL +DI + EE+ G
Sbjct: 721 VSIGVATILLITILFTEEDDILSHIDYG----SKFLRLASLTARLANDIKTYQEERAHGE 776
Query: 1634 NISAVESYAKDNPECTLEEVVAFLRDMTQGFMKNIVQHYFSTHTAPEQSQTPEDSFYFKR 1813
+SA++ Y KD PE T EE + ++ + + Y ++ P+ KR
Sbjct: 777 VVSAIQCYMKDRPEITEEEALKYVYGRMVNDLAELNSEYLKSNEMPQNC---------KR 827
Query: 1814 TTLEMTRTFNFFYSYGDGFGHLNDGLINQ-IDAILFQP 1924
+ R F GDG + + I + I LF+P
Sbjct: 828 LVFDTARVAQLFTMEGDGLTYSDTMEIKEHIKKCLFEP 865
Score = 62.8 bits (151), Expect(3) = 1e-52
Identities = 38/74 (51%), Positives = 49/74 (66%)
Frame = +2
Query: 248 DLIERLSLIDCLLCLGIDRLFQEEIRRDLDYVYMKAWNSSSGIGDGTKSPKANIVSTALA 427
DL ERL ++D + LGIDR F++EI+ LDYVY W+ GIG SP A+I TA+
Sbjct: 353 DLFERLWVVDTVERLGIDRHFKKEIKDALDYVY-SCWD-ERGIGWAKDSPIADIDDTAMG 410
Query: 428 FKLLRLHRYQNVSP 469
++LRLH Y NVSP
Sbjct: 411 LRILRLHGY-NVSP 423
Score = 62.4 bits (150), Expect(3) = 1e-52
Identities = 42/131 (32%), Positives = 64/131 (48%), Gaps = 6/131 (4%)
Frame = +3
Query: 444 SIDIKMSLQFQNGSGHGFLEEEISSSLFTRTSVAKALNLVRASYLQFHGETILEEAKTSC 623
S D+ + + +NG F+ + T+ V LN+ R S + F GETI+EEAK
Sbjct: 422 SPDVLKTFKDENGEFFCFMGQ-------TQRGVTDMLNVYRCSQVAFPGETIMEEAKLCT 474
Query: 624 TSFLNSFISLQQNVTESKDDFAR------LKQEVEHTLQYQWILTTPRWEARRFIDFYSA 785
+L +N E+ D F + ++ EVE+ L+Y W + PR E R +I Y
Sbjct: 475 ERYL-------RNALENADAFDKWAIKKNIRGEVEYALKYPWHRSMPRLEVRSYIGNYGP 527
Query: 786 NKDWLRYSLFL 818
N WL SL++
Sbjct: 528 NDVWLGKSLYM 538
>gb|AGN70885.1| levopimaradiene/abietadiene synthase, partial [Pseudolarix amabilis]
Length = 784
Score = 134 bits (336), Expect(3) = 3e-52
Identities = 99/338 (29%), Positives = 162/338 (47%), Gaps = 1/338 (0%)
Frame = +2
Query: 917 NMKILQLAKLDYNIVQAKLQKDLKEVSRWWNNTEISKLRNFRDGLVRSFLIPACGIYEPE 1096
N K L+LAKLD+N VQ+ QK+L+E+ RWW + S+L R+ + + PA ++EPE
Sbjct: 458 NEKYLELAKLDFNKVQSIHQKELREIRRWWESAGFSELNFTRERVTEIYFSPASFMFEPE 517
Query: 1097 LSGVRILTTKGCLGSYILDDVYDEFASQEELPIIIKAMEKLKLDANNGYCLKEHIITGCQ 1276
+ R + TK + ILDD+YD S ++L + +A+++ L + KE I
Sbjct: 518 FAMCREVYTKTSNFTLILDDLYDAHGSLDDLKLFSEAVKRWDLSLID-KMPKEMKICFVG 576
Query: 1277 FYLDANIQIITELNNIQGADTQKYLHQRIMGWAHASEQEAIWKFQGYVPTFEEYMRQGVE 1456
FY N +I E + QG D Y+ + A +EA W YVP+F+EY+
Sbjct: 577 FYNTFN-EIAEEGHKRQGRDVLGYIKKVWEIQLAAYTKEAEWSEAKYVPSFDEYIENASV 635
Query: 1457 SVAMLPLFLVAKLGLCSGFPLADLEMRPHGISTFDKLCALSCRLTDDIHDFAEEKEQGFN 1636
S+A+ + L++ L L HG S F L L+ RL +D + E+ QG
Sbjct: 636 SIALGTVVLISALFTGEVLTNHVLSKIDHG-SRFLHLMGLTGRLVNDTKTYQAERGQGEV 694
Query: 1637 ISAVESYAKDNPECTLEEVVAFLRDMTQGFMKNIVQHYFSTHTAPEQSQTPEDSFYFKRT 1816
SA++ Y KD+P + EE + + + + ++ + + + + P +R
Sbjct: 695 ASAIQCYMKDHPRISEEEALKHIYSVMKNSLEELNREFLNNKEVPNNC---------RRL 745
Query: 1817 TLEMTRTFNFFYSYGDGFGHLNDGLI-NQIDAILFQPI 1927
+ R FY GDG +D I + + LF+P+
Sbjct: 746 VFDTARIMQLFYMQGDGLTLSHDMEIKDHVKNCLFKPV 783
Score = 63.2 bits (152), Expect(3) = 3e-52
Identities = 41/131 (31%), Positives = 67/131 (51%), Gaps = 6/131 (4%)
Frame = +3
Query: 444 SIDIKMSLQFQNGSGHGFLEEEISSSLFTRTSVAKALNLVRASYLQFHGETILEEAKTSC 623
S D+ + + +NG FL + T+ V LN+ R S++ F GETI+EEAK
Sbjct: 336 SSDVLKTFRDENGEFFCFLGQ-------TQRGVTDMLNVNRCSHVSFPGETIMEEAKLCT 388
Query: 624 TSFLNSFISLQQNVTESKDDFAR------LKQEVEHTLQYQWILTTPRWEARRFIDFYSA 785
+L +N ++ D F + ++ EVE+ L+Y W + PR EAR +I+ Y
Sbjct: 389 ERYL-------RNALDNVDAFDKWAFKKNIRGEVEYALKYPWHKSMPRLEARSYIENYGP 441
Query: 786 NKDWLRYSLFL 818
N WL ++++
Sbjct: 442 NDVWLGKTVYM 452
Score = 58.5 bits (140), Expect(3) = 3e-52
Identities = 37/78 (47%), Positives = 48/78 (61%)
Frame = +2
Query: 233 CGAENDLIERLSLIDCLLCLGIDRLFQEEIRRDLDYVYMKAWNSSSGIGDGTKSPKANIV 412
C DL ERL +D + LGIDR F+EEI+ LDYVY W+ GIG ++P +I
Sbjct: 262 CHYPLDLFERLWAVDTVERLGIDRHFKEEIKDALDYVY-SHWD-KRGIGWARENPVPDID 319
Query: 413 STALAFKLLRLHRYQNVS 466
TA+ ++LRLH Y NVS
Sbjct: 320 DTAMGLRILRLHGY-NVS 336
>sp|Q675L4.1|TPSD1_PICAB RecName: Full=Levopimaradiene synthase, chloroplastic; AltName:
Full=Diterpene synthase; AltName: Full=PaTPS-LAS;
Includes: RecName: Full=Levopimaradiene synthase;
Includes: RecName: Full=Copalyl diphosphate synthase;
Flags: Precursor gi|44804521|gb|AAS47691.1|
levopimaradiene/abietadiene synthase [Picea abies]
Length = 859
Score = 131 bits (330), Expect(3) = 6e-52
Identities = 98/339 (28%), Positives = 166/339 (48%), Gaps = 2/339 (0%)
Frame = +2
Query: 917 NMKILQLAKLDYNIVQAKLQKDLKEVSRWWNNTEISKLRNFRDGLVRSFLIPACGIYEPE 1096
N K L+LAKLD+N VQ+ QK+L+++ RWW ++ ++L+ R+ + + PA ++EPE
Sbjct: 533 NEKYLELAKLDFNHVQSLHQKELRDLRRWWTSSGFTELKFTRERVTEIYFSPASFMFEPE 592
Query: 1097 LSGVRILTTKGCLGSYILDDVYDEFASQEELPIIIKAMEKLKLDANNGYCLKEHIITGCQ 1276
+ R + TK + ILDD+YD + ++L + +++K L + ++ I
Sbjct: 593 FATCRAVYTKTSNFTVILDDLYDAHGTLDDLKLFSDSVKKWDLSLVD-RMPQDMKICFMG 651
Query: 1277 FYLDANIQIITELNNIQGADTQKYLHQRIMGWAHASEQEAIWKFQGYVPTFEEYMRQGVE 1456
FY N +I E QG D Y+ A +EA W YVP+F+EY+
Sbjct: 652 FYNTFN-EIAEEGRKRQGRDVLGYIRNVWEIQLEAYTKEAEWSAARYVPSFDEYIDNASV 710
Query: 1457 SVAMLPLFLVAKLGLCSGFPLADLEMRPHGI-STFDKLCALSCRLTDDIHDFAEEKEQGF 1633
S+A+ + L++ L +G L D + G S F +L L+ RL +D + E+ QG
Sbjct: 711 SIALGTVVLIS--ALFTGEILTDDVLSKIGRGSRFLQLMGLTGRLVNDTKTYEAERGQGE 768
Query: 1634 NISAVESYAKDNPECTLEEVVAFLRDMTQGFMKNIVQHYFSTHTAPEQSQTPEDSFYFKR 1813
SAV+ Y KD+PE + EE + + + + + + + + + P+ +R
Sbjct: 769 VASAVQCYMKDHPEISEEEALKHVYTVMENALDELNREFVNNREVPDSC---------RR 819
Query: 1814 TTLEMTRTFNFFYSYGDGFGHLNDGLINQ-IDAILFQPI 1927
E R FY GDG ++ I + + LFQP+
Sbjct: 820 LVFETARIMQLFYMDGDGLTLSHETEIKEHVKNCLFQPV 858
Score = 64.3 bits (155), Expect(3) = 6e-52
Identities = 42/131 (32%), Positives = 66/131 (50%), Gaps = 6/131 (4%)
Frame = +3
Query: 444 SIDIKMSLQFQNGSGHGFLEEEISSSLFTRTSVAKALNLVRASYLQFHGETILEEAKTSC 623
S D+ + + +NG FL + T+ V LN+ R S++ F GETI+EEAKT
Sbjct: 411 SSDVLKTFRDENGEFFCFLGQ-------TQRGVTDMLNVNRCSHVAFPGETIMEEAKTCT 463
Query: 624 TSFLNSFISLQQNVTESKDDFAR------LKQEVEHTLQYQWILTTPRWEARRFIDFYSA 785
+L +N E F + ++ EVE+ L+Y W + PR EAR +I+ Y
Sbjct: 464 ERYL-------RNALEDVGAFDKWALKKNIRGEVEYALKYPWHRSMPRLEARSYIEHYGP 516
Query: 786 NKDWLRYSLFL 818
N WL ++++
Sbjct: 517 NDVWLGKTMYM 527
Score = 58.9 bits (141), Expect(3) = 6e-52
Identities = 37/78 (47%), Positives = 48/78 (61%)
Frame = +2
Query: 233 CGAENDLIERLSLIDCLLCLGIDRLFQEEIRRDLDYVYMKAWNSSSGIGDGTKSPKANIV 412
C DL ERL +D + LGIDR F+EEI+ LDYVY W+ GIG ++P +I
Sbjct: 337 CHYPLDLFERLWAVDTIERLGIDRHFKEEIKDALDYVY-SHWD-ERGIGWARENPVPDID 394
Query: 413 STALAFKLLRLHRYQNVS 466
TA+ ++LRLH Y NVS
Sbjct: 395 DTAMGLRILRLHGY-NVS 411