BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Ephedra26_contig00006670
         (1212 letters)

Database: ./nr 
           37,332,560 sequences; 13,225,080,153 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_006857151.1| hypothetical protein AMTR_s00065p00162460 [A...   203   1e-49
gb|AAC63111.1| steroid sulfotransferase 1 [Brassica napus]            184   6e-44
ref|XP_006348647.1| PREDICTED: cytosolic sulfotransferase 12-lik...   184   8e-44
ref|XP_002267209.1| PREDICTED: flavonol sulfotransferase-like [V...   183   1e-43
ref|XP_006348649.1| PREDICTED: cytosolic sulfotransferase 12-lik...   182   2e-43
ref|XP_006292601.1| hypothetical protein CARUB_v10018842mg [Caps...   182   3e-43
gb|EOY10476.1| Sulfotransferase 2A, putative [Theobroma cacao]        180   9e-43
ref|XP_006388018.1| hypothetical protein POPTR_0396s00220g [Popu...   179   2e-42
ref|XP_006348794.1| PREDICTED: cytosolic sulfotransferase 12-lik...   179   2e-42
gb|AAR14296.1| steroid sulfotransferase 4 [Brassica napus]            179   2e-42
ref|XP_006378523.1| sulfotransferase family protein [Populus tri...   178   3e-42
ref|XP_004297203.1| PREDICTED: cytosolic sulfotransferase 12-lik...   178   3e-42
gb|ABA97383.1| Sulfotransferase domain containing protein, expre...   178   3e-42
ref|XP_002334799.1| predicted protein [Populus trichocarpa]           178   3e-42
gb|EOY01595.1| Flavonol 4'-sulfotransferase, putative [Theobroma...   178   5e-42
ref|XP_002527846.1| Flavonol 3-sulfotransferase, putative [Ricin...   178   5e-42
gb|EAY97091.1| hypothetical protein OsI_19013 [Oryza sativa Indi...   178   5e-42
ref|XP_006339394.1| PREDICTED: cytosolic sulfotransferase 12-lik...   177   6e-42
ref|XP_004235426.1| PREDICTED: cytosolic sulfotransferase 5-like...   177   6e-42
gb|EOY01597.1| P-loop containing nucleoside triphosphate hydrola...   177   1e-41

>ref|XP_006857151.1| hypothetical protein AMTR_s00065p00162460 [Amborella trichopoda]
            gi|548861234|gb|ERN18618.1| hypothetical protein
            AMTR_s00065p00162460 [Amborella trichopoda]
          Length = 317

 Score =  203 bits (516), Expect = 1e-49
 Identities = 112/279 (40%), Positives = 166/279 (59%), Gaps = 11/279 (3%)
 Frame = +1

Query: 199  KEFQVQHGDVLLASVLKSGTTWMKSLLHSIMACVHGF------PNLLGDQNPHYIVPNIE 360
            K F  +  D+ +AS  K GTTW+KSL+ SI+   H        PN L   +PH ++ N+E
Sbjct: 48   KHFSARSTDIFVASYPKCGTTWLKSLIFSIIN-QHSLNQEKESPNPLLSTSPHDLIHNLE 106

Query: 361  MS--SVDSWPHLT---SPRLLSTHLPLHALPRQIPHDDQLKIVYVTRNPRDTFVSFWKFW 525
            +   S+D  P +T   SPRL STH    +LP  + H    +IVY+TRNPRD+ VSFW F 
Sbjct: 107  LEVYSMDPIPDITGIPSPRLFSTHHSYGSLPESLKHCG-CQIVYITRNPRDSIVSFWHFT 165

Query: 526  ERLAESAVKLGIQRHIPKEVLLEKFCAGHFQGGPFSDNVLSYLRESRRRPDKVLFVTYED 705
            ++ +   ++     ++  E   E+FC G    GPF ++VL YL+ S+  P+KV+F+TYE+
Sbjct: 166  KKFSYHGLE-----YLSFEETFERFCEGALPFGPFFEHVLQYLKASKEMPEKVMFLTYEE 220

Query: 706  LHADCQAVICKIGNFLGYPSDVVEGNVSQIAKRCSFRNISSLDINKKDESSEGRYPPNSI 885
            + AD + V+ ++  FLG P   VE  V +I + CSF  +S+L++NK +E++  R  PN++
Sbjct: 221  MIADTKGVVKRVTAFLGRP---VEEEVEKIVEMCSFEKLSNLEVNKSEENTSKRRYPNNV 277

Query: 886  FFRSGETGGWREHFTPAMIERMRQEIEQKLEDEGLYFDY 1002
            FFR G  G W  HFTP MIER+    +QKL+  G  F +
Sbjct: 278  FFRKGAVGDWMNHFTPEMIERLDHITQQKLQGSGFEFRF 316


>gb|AAC63111.1| steroid sulfotransferase 1 [Brassica napus]
          Length = 323

 Score =  184 bits (467), Expect = 6e-44
 Identities = 110/274 (40%), Positives = 154/274 (56%), Gaps = 8/274 (2%)
 Frame = +1

Query: 199 KEFQVQHGDVLLASVLKSGTTWMKSLLHSIMACVHGFP----NLLGDQNPHYIVPNIEMS 366
           K F+    DV+LA++ KSGTTW+K+LL +++   H FP    + L   NPH +VP +E  
Sbjct: 58  KHFEANDSDVILATLAKSGTTWLKALLFALIHR-HKFPVSGKHPLLVTNPHSLVPYLEGD 116

Query: 367 SVDS----WPHLTSPRLLSTHLPLHALPRQIPHDDQLKIVYVTRNPRDTFVSFWKFWERL 534
              S    +  L SPRL+ THL  H+LP  I      KI+Y  RNP+D FVS W F  +L
Sbjct: 117 YCSSPEVNFAELPSPRLMQTHLTHHSLPVSIK-SSSCKIIYCCRNPKDMFVSIWHFGRKL 175

Query: 535 AESAVKLGIQRHIPKEVLLEKFCAGHFQGGPFSDNVLSYLRESRRRPDKVLFVTYEDLHA 714
           A            P E  +  FC G F GGPF D+VL Y  ES + P+KVLFVTYE+L  
Sbjct: 176 APEKTA-----EYPIETAVAAFCKGKFIGGPFWDHVLEYWYESLKNPNKVLFVTYEELKK 230

Query: 715 DCQAVICKIGNFLGYPSDVVEGNVSQIAKRCSFRNISSLDINKKDESSEGRYPPNSIFFR 894
             +  + +I  F+G      E  VS+I K CSF ++SSL++N++ +   G    ++ FFR
Sbjct: 231 QTEVEVKRIAEFIGC-GFTAEEEVSEIVKLCSFESLSSLEVNRQGKLPNG--IESNAFFR 287

Query: 895 SGETGGWREHFTPAMIERMRQEIEQKLEDEGLYF 996
            GETGGWR+  + ++ + + +  EQK    GL F
Sbjct: 288 KGETGGWRDTLSESLADVIDRTTEQKFGGSGLKF 321


>ref|XP_006348647.1| PREDICTED: cytosolic sulfotransferase 12-like [Solanum tuberosum]
          Length = 330

 Score =  184 bits (466), Expect = 8e-44
 Identities = 104/284 (36%), Positives = 162/284 (57%), Gaps = 16/284 (5%)
 Frame = +1

Query: 199  KEFQVQHGDVLLASVLKSGTTWMKSLL--------HSIMACVHGFPNLLGDQNPHYIVPN 354
            ++FQ Q  D++L +  KSGTTW+KSLL        H +    H     L D+NPH ++P 
Sbjct: 58   QQFQAQDSDIILVTPPKSGTTWLKSLLFVLVNRMKHPVFEQNHP----LLDKNPHDLIPF 113

Query: 355  IEMS-----SVDSWPHLTSPRLLSTHLPLHALPRQIPHDDQLKIVYVTRNPRDTFVSFWK 519
            +E++      V ++   TSPRLLSTH+P  +LP+ I  D + K+VY+ RNP+DTF+S W 
Sbjct: 114  MELTLYVDGHVPNFSAFTSPRLLSTHVPFASLPKSIK-DSKTKLVYLCRNPKDTFISMWH 172

Query: 520  FWERLAESAVKLGIQRHIPKEVLLEKFCAGHFQGGPFSDNVLSYLRESRRRPDKVLFVTY 699
            F   L     +L  +     E + E FC G    GPF +++L Y ++S  +P+KVLF+ Y
Sbjct: 173  FTNNL-----RLHHKDIHSIEEIFESFCKGVSPYGPFWNHILDYWKQSIEKPNKVLFLMY 227

Query: 700  EDLHADCQAVICKIGNFLGYPSDVVEGN---VSQIAKRCSFRNISSLDINKKDESSEGRY 870
            E++    +  + ++  +L  P  + E N   + +I + CSF N+S+L++N   + S G  
Sbjct: 228  EEIKVQPKLQLKRLAEYLECPFSIEEENCGVMDEILRMCSFENLSNLEVNTNGKLSTG-- 285

Query: 871  PPNSIFFRSGETGGWREHFTPAMIERMRQEIEQKLEDEGLYFDY 1002
              N +FFR GE G W+ +FT  M E++   IEQK ++ G+ F Y
Sbjct: 286  AENKVFFRKGEVGDWKNYFTIEMSEKLNHIIEQKFQESGIKFSY 329


>ref|XP_002267209.1| PREDICTED: flavonol sulfotransferase-like [Vitis vinifera]
          Length = 333

 Score =  183 bits (465), Expect = 1e-43
 Identities = 107/279 (38%), Positives = 157/279 (56%), Gaps = 12/279 (4%)
 Frame = +1

Query: 199 KEFQVQHGDVLLASVLKSGTTWMKSLLHSIMACVHGFPNL----LGDQNPHYIVPNIEM- 363
           + FQ    D+LL +  KSGTTW+K++  +++  V  FP+     L   NPH +VP +E+ 
Sbjct: 62  QHFQAHDTDILLVTTPKSGTTWLKAMAFALLKRVR-FPDTRHHPLLTNNPHELVPFLEIK 120

Query: 364 ----SSVDSWPHLTSPRLLSTHLPLHALPRQIPHDDQLKIVYVTRNPRDTFVSFWKFWER 531
               S V      T+PRL STHLP  +LP  + +    K+VY+ RNPRDTFVSFW+F  +
Sbjct: 121 LYAESKVPDLTSFTAPRLFSTHLPFTSLPESV-NTSGCKLVYLCRNPRDTFVSFWQFTNK 179

Query: 532 LAESAVKLGIQRHIPKEVLLEKFCAGHFQGGPFSDNVLSYLRESRRRPDKVLFVTYEDLH 711
           L     +L      P E + +KFC G    GPF D+VL Y +ES  +P+K+LF+ YE++ 
Sbjct: 180 L-----RLENMGTNPLEEVFDKFCWGVSLSGPFWDHVLGYWKESLEKPEKILFLKYEEMK 234

Query: 712 ADCQAVICKIGNFLG---YPSDVVEGNVSQIAKRCSFRNISSLDINKKDESSEGRYPPNS 882
           A     + ++  FLG    P +     V +I + CSF N+S+L++NK  + S G    +S
Sbjct: 235 AQPHVQLRRLAEFLGCPFCPEEETRCVVDEILQLCSFENLSNLEVNKNGKLSSGE--EHS 292

Query: 883 IFFRSGETGGWREHFTPAMIERMRQEIEQKLEDEGLYFD 999
            FFR GE G W  + T  M++R+    E+KL   GL F+
Sbjct: 293 AFFRRGEVGDWVNYLTAEMLDRLDHITEEKLHGSGLKFE 331


>ref|XP_006348649.1| PREDICTED: cytosolic sulfotransferase 12-like [Solanum tuberosum]
          Length = 335

 Score =  182 bits (462), Expect = 2e-43
 Identities = 104/282 (36%), Positives = 161/282 (57%), Gaps = 14/282 (4%)
 Frame = +1

Query: 199  KEFQVQHGDVLLASVLKSGTTWMKSLLHSIMACV------HGFPNLLGDQNPHYIVPNIE 360
            ++FQ Q  D++L +  KSGTTW+KSLL  ++  +         P L+  +NPH ++P +E
Sbjct: 63   QQFQAQDSDIILVTTPKSGTTWLKSLLFVLVNRMKYPVFEQNHPLLV--KNPHDLIPFLE 120

Query: 361  MSS-----VDSWPHLTSPRLLSTHLPLHALPRQIPHDDQLKIVYVTRNPRDTFVSFWKFW 525
            +       V ++   TSPRLLSTH+P  +LP+ +  D + K+VY+ RNP+DTF+S W F 
Sbjct: 121  IKLYVDGLVPNFASFTSPRLLSTHMPFASLPKSV-QDSKTKLVYLCRNPKDTFISLWHFT 179

Query: 526  ERLAESAVKLGIQRHIPKEVLLEKFCAGHFQGGPFSDNVLSYLRESRRRPDKVLFVTYED 705
              L     +L  +   P E   E FC G    GPF ++VL Y ++S  +P+KVLF+ YE+
Sbjct: 180  NNL-----RLHHKDIHPIEEKFESFCKGVSLYGPFWNHVLDYWKQSIEKPNKVLFLMYEE 234

Query: 706  LHADCQAVICKIGNFLGYPSDVVEGN---VSQIAKRCSFRNISSLDINKKDESSEGRYPP 876
            +    +  + ++  FL  P  + E N   + +I + CSF N+S+L++N   + S G    
Sbjct: 235  IKVQPKLQLKRLAEFLECPFSIEEENCGVMDEILRMCSFENLSNLEVNADGKLSTGE--E 292

Query: 877  NSIFFRSGETGGWREHFTPAMIERMRQEIEQKLEDEGLYFDY 1002
            N+ FFR GE G W+ +FT  M E++   IEQK +  G+ F Y
Sbjct: 293  NNAFFRKGEVGDWKNYFTIEMSEKLNHIIEQKFQGSGIKFSY 334


>ref|XP_006292601.1| hypothetical protein CARUB_v10018842mg [Capsella rubella]
           gi|482561308|gb|EOA25499.1| hypothetical protein
           CARUB_v10018842mg [Capsella rubella]
          Length = 324

 Score =  182 bits (461), Expect = 3e-43
 Identities = 109/279 (39%), Positives = 155/279 (55%), Gaps = 13/279 (4%)
 Frame = +1

Query: 199 KEFQVQHGDVLLASVLKSGTTWMKSLLHSIMACVHGFP------NLLGDQNPHYIVPNIE 360
           K F+ +  D++L +  KSGTTW+K+L+ +++   H FP      + L   NPH +VP +E
Sbjct: 57  KRFEAKDSDIILVTNPKSGTTWLKALVFALLNR-HKFPVPSSGNHPLLVTNPHLLVPFLE 115

Query: 361 MSSVDS----WPHLTSPRLLSTHLPLHALPRQIPHDDQLKIVYVTRNPRDTFVSFWKFWE 528
               +S    +  L +PRL++TH+P  +LP  +      KIVY  RNP+D FVS W F +
Sbjct: 116 GVYYESPDFDFLGLPTPRLMNTHIPYPSLPESVK-SSSCKIVYCCRNPKDMFVSLWHFGK 174

Query: 529 RLAESAVKLGIQRHIPKEVLLEKFCAGHFQGGPFSDNVLSYLRESRRRPDKVLFVTYEDL 708
           +LA            P E  +E FC G F GGPF D++L Y   SR  PDKV+FVTYEDL
Sbjct: 175 KLAPKETA-----DYPIEKAVEAFCEGKFIGGPFWDHILEYWYASRENPDKVMFVTYEDL 229

Query: 709 HADCQAVICKIGNFLGYPSDVVEGNVSQIAKRCSFRNISSLDINKKDESSEGRYP---PN 879
                  I +I  FLG    + EG V +I K CSF ++S+L++N+     EG+ P     
Sbjct: 230 KKQTGVEIKRIAEFLGC-GFIEEGQVEEIVKLCSFESLSNLEVNR-----EGKLPNGIET 283

Query: 880 SIFFRSGETGGWREHFTPAMIERMRQEIEQKLEDEGLYF 996
             FFR GE GGWR+  + ++   + + IE K +D GL F
Sbjct: 284 KSFFRKGEIGGWRDTLSESLAAEIDRTIEDKFQDSGLKF 322


>gb|EOY10476.1| Sulfotransferase 2A, putative [Theobroma cacao]
          Length = 339

 Score =  180 bits (457), Expect = 9e-43
 Identities = 100/278 (35%), Positives = 147/278 (52%), Gaps = 10/278 (3%)
 Frame = +1

Query: 199  KEFQVQHGDVLLASVLKSGTTWMKSLLHSIMACVHGF--PNLLGDQNPHYIVPNIEM--- 363
            K FQ    D+ L S+ K GTTWMK+L+ +I+   H     N L    PH  VP +E+   
Sbjct: 65   KHFQALDNDIFLTSLPKCGTTWMKALIFTIVNRNHFELKNNPLLSLGPHQAVPYLELDLY 124

Query: 364  --SSVDSWPHLTSPRLLSTHLPLHALPRQIPHDDQLKIVYVTRNPRDTFVSFWKFWERLA 537
              +      ++  PR+ STH P  +LP  I      KIVY+ RNP D F+S+W F + L 
Sbjct: 125  LKNHSPDLENIPQPRIFSTHTPYASLPPSIKECSTPKIVYICRNPMDMFISYWHFTDILR 184

Query: 538  ESAVKLGIQRHIPKEVLLEKFCAGHFQGGPFSDNVLSYLRESRRRPDKVLFVTYEDLHAD 717
               V       +P +   E FC G    GPF D+VL Y +  +  P+ ++F+ YEDL  D
Sbjct: 185  SENVD-----PLPLDEAFEMFCQGIHGFGPFPDHVLGYWKAKQENPNNIMFLKYEDLKKD 239

Query: 718  CQAVICKIGNFLGYP---SDVVEGNVSQIAKRCSFRNISSLDINKKDESSEGRYPPNSIF 888
                + K+ NFLG+P    +  +G   +IA  CSF N+  +++NK  +   G   PN+ F
Sbjct: 240  IVFHVKKLANFLGFPFSKEEERQGEAEEIAMLCSFENLKGMEVNKSGKQPFG--APNTAF 297

Query: 889  FRSGETGGWREHFTPAMIERMRQEIEQKLEDEGLYFDY 1002
            FR GE G W  + TP+M+ER+++ +++KL    L F Y
Sbjct: 298  FRKGEVGDWSNYLTPSMVERLQKLVQEKLNKSDLTFKY 335


>ref|XP_006388018.1| hypothetical protein POPTR_0396s00220g [Populus trichocarpa]
           gi|550309250|gb|ERP46932.1| hypothetical protein
           POPTR_0396s00220g [Populus trichocarpa]
          Length = 304

 Score =  179 bits (455), Expect = 2e-42
 Identities = 108/278 (38%), Positives = 157/278 (56%), Gaps = 14/278 (5%)
 Frame = +1

Query: 199 KEFQVQHGDVLLASVLKSGTTWMKSLLHSIMACVHGFPNL----LGDQNPHYIVPNIEMS 366
           K FQ Q GD+ L +  KSG+TW+K+++ S++  V  FP+     L   NPH +VP +EM 
Sbjct: 26  KHFQAQDGDIFLVTTPKSGSTWLKAVMFSLVNRV-SFPDTKQHPLLTNNPHALVPFLEME 84

Query: 367 SVDS----WPHLTSPRLLSTHLPLHALPRQIPHDDQLKIVYVTRNPRDTFVSFWKFWERL 534
            +      + + T PRL STHLPL +LPR +  D   K+VY+ RNP+DTFVSFW F  +L
Sbjct: 85  YIGKENLDFTNFTFPRLFSTHLPLLSLPRSV-EDSDCKLVYLCRNPKDTFVSFWHFTNKL 143

Query: 535 AE---SAVKLGIQRHIPKEVLLEKFCAGHFQGGPFSDNVLSYLRESRRRPDKVLFVTYED 705
                SA  L        E   +KFC G    GPF D+VL Y +ES  RP +V F+ YE+
Sbjct: 144 TPIGWSATSL--------EETFDKFCRGVSLYGPFWDHVLGYWKESLERPHRVFFLKYEE 195

Query: 706 LHADCQAVICKIGNFLGYPSDVVEGN---VSQIAKRCSFRNISSLDINKKDESSEGRYPP 876
           +  + +  + ++  FLG P  + E N   + +I + CSF N+S+L++NK  +   G+   
Sbjct: 196 MKREPRIQLRRLSEFLGCPFSLEEENSGVLDEILELCSFENLSNLEVNKIGKLHSGQ--E 253

Query: 877 NSIFFRSGETGGWREHFTPAMIERMRQEIEQKLEDEGL 990
           + +FFR GE G    + T  M+E++    EQKL   GL
Sbjct: 254 HRVFFRRGEVGDSMNYLTAEMVEKIDMITEQKLHCHGL 291


>ref|XP_006348794.1| PREDICTED: cytosolic sulfotransferase 12-like [Solanum tuberosum]
          Length = 332

 Score =  179 bits (454), Expect = 2e-42
 Identities = 105/282 (37%), Positives = 158/282 (56%), Gaps = 14/282 (4%)
 Frame = +1

Query: 199  KEFQVQHGDVLLASVLKSGTTWMKSLLHSIMACV------HGFPNLLGDQNPHYIVPNIE 360
            K+FQ Q  D+ L +  KSGT W+KSLL +++  +         P L  ++NPH +VP +E
Sbjct: 60   KQFQAQDSDIFLVTNPKSGTIWLKSLLFALVNRMKHPMFEQNHPLL--NKNPHDLVPFLE 117

Query: 361  MS-----SVDSWPHLTSPRLLSTHLPLHALPRQIPHDDQLKIVYVTRNPRDTFVSFWKFW 525
            ++      V  +   T+PRLLSTHLP  +LP+    D + K+VY+ RNP+DTF+S W F 
Sbjct: 118  LTLYVDGQVPDFSSFTTPRLLSTHLPFASLPKSA-QDSKTKLVYLCRNPKDTFISMWHFS 176

Query: 526  ERLAESAVKLGIQRHIPKEVLLEKFCAGHFQGGPFSDNVLSYLRESRRRPDKVLFVTYED 705
              L     +L  +     E + + +C G    GPF D+VL Y ++S   P+KVLF+ YE+
Sbjct: 177  NNL-----RLHHKDTNSIEEMFDLYCKGMSVYGPFWDHVLDYWKQSIENPNKVLFLMYEE 231

Query: 706  LHADCQAVICKIGNFLGYPSDVVEGN---VSQIAKRCSFRNISSLDINKKDESSEGRYPP 876
            +    +  + ++  FL  P  + E N   V +I + CSF+N+S+L++N   + S G    
Sbjct: 232  IKEQPKMHLKRLAEFLECPFSIKEENCGVVDEILRMCSFKNLSNLEVNTTGKLSTGE--E 289

Query: 877  NSIFFRSGETGGWREHFTPAMIERMRQEIEQKLEDEGLYFDY 1002
            N +FFR GE G W+ +FT  M E+    IEQK +  GL F Y
Sbjct: 290  NKVFFRRGEIGDWKNYFTTEMSEKFNYIIEQKFQGSGLKFSY 331


>gb|AAR14296.1| steroid sulfotransferase 4 [Brassica napus]
          Length = 323

 Score =  179 bits (454), Expect = 2e-42
 Identities = 107/277 (38%), Positives = 152/277 (54%), Gaps = 9/277 (3%)
 Frame = +1

Query: 193 HSKEFQVQHGDVLLASVLKSGTTWMKSLLHSIMACVHGFPNLLGDQ-----NPHYIVPNI 357
           + K F+ +  D++L +  KSGTTW+K+L+ S++   H FP   GD      NPH ++P +
Sbjct: 55  YQKHFEAKDSDIILVTNPKSGTTWLKALVFSLINR-HKFPVSSGDHPLLVTNPHLLIPFL 113

Query: 358 EMSSVDS----WPHLTSPRLLSTHLPLHALPRQIPHDDQLKIVYVTRNPRDTFVSFWKFW 525
           E    +S    +  L SPRL++TH+ L +LP  +      KIVY  RNP+D FVS W F 
Sbjct: 114 EGVYYESPNFDFTELPSPRLMNTHISLLSLPESVK-SSSCKIVYCCRNPKDMFVSLWHFG 172

Query: 526 ERLAESAVKLGIQRHIPKEVLLEKFCAGHFQGGPFSDNVLSYLRESRRRPDKVLFVTYED 705
           ++LA            P E  +E FC G F GGPF D+VL Y   S   P+KVLFVTYE+
Sbjct: 173 KKLASQETA-----DYPIEKAVEAFCQGKFIGGPFWDHVLEYWYASLENPNKVLFVTYEE 227

Query: 706 LHADCQAVICKIGNFLGYPSDVVEGNVSQIAKRCSFRNISSLDINKKDESSEGRYPPNSI 885
           L       I +I  FLG    + E  V  I K CSF ++SSL+ N++ +   G       
Sbjct: 228 LKKQTGDTIKRIAEFLGC-GFIEEEEVGGIVKLCSFESLSSLEANREGKLPNG--VETKA 284

Query: 886 FFRSGETGGWREHFTPAMIERMRQEIEQKLEDEGLYF 996
           FFR GE GGWR+  + ++ E + + +E+K +  GL F
Sbjct: 285 FFRKGEVGGWRDTLSESLAEEIDRTMEEKFQGSGLKF 321


>ref|XP_006378523.1| sulfotransferase family protein [Populus trichocarpa]
           gi|550329829|gb|ERP56320.1| sulfotransferase family
           protein [Populus trichocarpa]
          Length = 316

 Score =  178 bits (452), Expect = 3e-42
 Identities = 108/280 (38%), Positives = 157/280 (56%), Gaps = 14/280 (5%)
 Frame = +1

Query: 199 KEFQVQHGDVLLASVLKSGTTWMKSLLHSIMACVHGFPNL----LGDQNPHYIVPNIEMS 366
           K FQ Q GD+ L +  KSG+TW+K+++ S++  V  FP+     L   NPH +VP +EM 
Sbjct: 49  KHFQAQDGDIFLVTTPKSGSTWLKAVMFSLVNRV-SFPDTKQHPLLTNNPHALVPFLEME 107

Query: 367 SVDS----WPHLTSPRLLSTHLPLHALPRQIPHDDQLKIVYVTRNPRDTFVSFWKFWERL 534
            +      + + T PRL STHLPL +LPR +  D   K+VY+ RNP+DTFVSFW F  +L
Sbjct: 108 YIGKENLDFTNFTFPRLFSTHLPLLSLPRSV-EDSDCKLVYLCRNPKDTFVSFWHFTNKL 166

Query: 535 AE---SAVKLGIQRHIPKEVLLEKFCAGHFQGGPFSDNVLSYLRESRRRPDKVLFVTYED 705
                SA  L        E   +KFC G    GPF D+VL Y +ES  RP +V F+ YE+
Sbjct: 167 RPIDWSATSL--------EETFDKFCRGVSLYGPFWDHVLGYWKESLERPQRVFFMKYEE 218

Query: 706 LHADCQAVICKIGNFLGYPSDVVEGN---VSQIAKRCSFRNISSLDINKKDESSEGRYPP 876
           +  + +  + ++  FLG P  + E N   + +I + CSF N+S+L++NK  +   G+   
Sbjct: 219 MKNEPRIQLRRLSEFLGCPFSLEEENSGVLDEILELCSFENLSNLEVNKIGKLHSGQ--E 276

Query: 877 NSIFFRSGETGGWREHFTPAMIERMRQEIEQKLEDEGLYF 996
           + +FFR GE G    + T  M+ ++    EQKL   GL F
Sbjct: 277 HQVFFRRGEVGDSMNYLTAEMVGKIDMITEQKLHCHGLKF 316


>ref|XP_004297203.1| PREDICTED: cytosolic sulfotransferase 12-like [Fragaria vesca
           subsp. vesca]
          Length = 334

 Score =  178 bits (452), Expect = 3e-42
 Identities = 110/281 (39%), Positives = 153/281 (54%), Gaps = 15/281 (5%)
 Frame = +1

Query: 199 KEFQVQHGDVLLASVLKSGTTWMKSLLHSIMA---CVHGFP--NLLGDQNPHYIVPNIEM 363
           + F+ Q  D+LL +  KSGTTW+K++L S++      H  P  +    +NPH +VP +E+
Sbjct: 62  ENFRAQDSDILLVTTPKSGTTWLKAILFSLVKRGEYYHPDPKDHPFLTKNPHVLVPFLEL 121

Query: 364 S-----SVDSWPHLTSPRLLSTHLPLHALPRQIPHDDQLKIVYVTRNPRDTFVSFWKFWE 528
                  V        PRL STHLPL +LP  +  D   K+VY+ RNP+DTFVS W F  
Sbjct: 122 GLYLQKPVPDLASYAPPRLFSTHLPLVSLPESVK-DSACKVVYLCRNPKDTFVSLWHFAN 180

Query: 529 RLAESAVKLGIQRHIPKEVLLEKFCAGHFQGGPFSDNVLSYLRESRRRPDKVLFVTYEDL 708
           RL   +     +  I  E  LE FC G    GPF D+VL Y  ES +RP  V F+ YE++
Sbjct: 181 RLRPES-----RGSISLEEALELFCRGVSLYGPFWDHVLGYWNESLKRPQSVFFIKYEEM 235

Query: 709 HADCQA--VICKIGNFLG---YPSDVVEGNVSQIAKRCSFRNISSLDINKKDESSEGRYP 873
             + +A   + K+  FLG    P +  +G V  I K CSF  +S+LD+NK+ + S G   
Sbjct: 236 KEEEEAGSHLRKLAEFLGCPFSPEEEAQGVVGNILKHCSFETLSNLDVNKRGKLSSGE-- 293

Query: 874 PNSIFFRSGETGGWREHFTPAMIERMRQEIEQKLEDEGLYF 996
            +S FFR GE G WR   T  M E++ +  E+KL+  GL F
Sbjct: 294 EHSAFFRRGEAGDWRNQLTAEMAEQIDRITEEKLQGSGLKF 334


>gb|ABA97383.1| Sulfotransferase domain containing protein, expressed [Oryza sativa
           Japonica Group] gi|215687276|dbj|BAG91841.1| unnamed
           protein product [Oryza sativa Japonica Group]
          Length = 338

 Score =  178 bits (452), Expect = 3e-42
 Identities = 106/308 (34%), Positives = 161/308 (52%), Gaps = 14/308 (4%)
 Frame = +1

Query: 115 PMTKTIRLEQHN-TWIRDFALPAFNAMHSKEFQVQHGDVLLASVLKSGTTWMKSLLHSIM 291
           P    +RL  +   W+ ++ LP   A+       +HGDV+LAS  K GTTW+K+L  +++
Sbjct: 43  PSYPKLRLRHYQGMWLMEYTLPGIMAIQRSFVPRRHGDVVLASPGKCGTTWLKALAFAVL 102

Query: 292 ACVHGFPNLLGDQ------NPHYIVPNIEMSSVDSW----PHLTSPRLLSTHLPLHALPR 441
           A   G  +   D+      NPH  VP +E +  + W      L SPRL+STH+   ALP+
Sbjct: 103 A--RGAYSPASDRHPLLRLNPHDCVPFMEGAISEGWGGKIDELPSPRLMSTHMQHAALPK 160

Query: 442 QIPHDDQLKIVYVTRNPRDTFVSFWKFWERLAESAVKLGIQRHIPKEVLLEKFCAGHFQG 621
            I  +   K+VY+ R P+D  VS W F+           I+  +  + + E  C G F  
Sbjct: 161 SIADEPGCKVVYICREPKDILVSAWHFFRI---------IEPDLSFQEVFEAACDGKFLT 211

Query: 622 GPFSDNVLSYLRESRRRPDKVLFVTYEDLHADCQAVICKIGNFLGYPSDVVE---GNVSQ 792
           G   D+++ Y    +  P+KVLF+ YEDL  D   ++ K+ +FLG P    E   G V+ 
Sbjct: 212 GAIWDHIIGYWNACKANPEKVLFLVYEDLLRDPANIVRKLADFLGQPFSSTEEEAGLVTD 271

Query: 793 IAKRCSFRNISSLDINKKDESSEGRYPPNSIFFRSGETGGWREHFTPAMIERMRQEIEQK 972
           I + CSF N+ SL++NK  E+S     PN+ +FR G+ G W+ H TP M+E     +++K
Sbjct: 272 IVRLCSFENLKSLEVNKMGEASFAF--PNASYFRKGKAGDWKIHMTPEMVECFDTIVKEK 329

Query: 973 LEDEGLYF 996
           +   GL F
Sbjct: 330 MHGSGLVF 337


>ref|XP_002334799.1| predicted protein [Populus trichocarpa]
          Length = 316

 Score =  178 bits (452), Expect = 3e-42
 Identities = 108/280 (38%), Positives = 157/280 (56%), Gaps = 14/280 (5%)
 Frame = +1

Query: 199 KEFQVQHGDVLLASVLKSGTTWMKSLLHSIMACVHGFPNL----LGDQNPHYIVPNIEMS 366
           K FQ Q GD+ L +  KSG+TW+K+++ S++  V  FP+     L   NPH +VP +EM 
Sbjct: 49  KHFQAQDGDIFLVTTPKSGSTWLKAVMFSLVNRV-SFPDTKQHPLLTNNPHALVPFLEME 107

Query: 367 SVDS----WPHLTSPRLLSTHLPLHALPRQIPHDDQLKIVYVTRNPRDTFVSFWKFWERL 534
            +      + + T PRL STHLPL +LPR +  D   K+VY+ RNP+DTFVSFW F  +L
Sbjct: 108 YIGKENLDFTNFTFPRLFSTHLPLLSLPRSV-EDSDCKLVYLCRNPKDTFVSFWHFTNKL 166

Query: 535 AE---SAVKLGIQRHIPKEVLLEKFCAGHFQGGPFSDNVLSYLRESRRRPDKVLFVTYED 705
                SA  L        E   +KFC G    GPF D+VL Y +ES  RP +V F+ YE+
Sbjct: 167 RPIDWSATSL--------EETFDKFCRGVSLYGPFWDHVLGYWKESLERPQRVFFMKYEE 218

Query: 706 LHADCQAVICKIGNFLGYPSDVVEGN---VSQIAKRCSFRNISSLDINKKDESSEGRYPP 876
           +  + +  + ++  FLG P  + E N   + +I + CSF N+S+L++NK  +   G+   
Sbjct: 219 MKNEPRIQLRRLSEFLGCPFSLEEENSGVLDEILELCSFENLSNLEVNKIGKLHSGQ--E 276

Query: 877 NSIFFRSGETGGWREHFTPAMIERMRQEIEQKLEDEGLYF 996
           + +FFR GE G    + T  M+ ++    EQKL   GL F
Sbjct: 277 HQVFFRRGEVGDSMNYLTAEMVGKIDMITEQKLHCHGLKF 316


>gb|EOY01595.1| Flavonol 4'-sulfotransferase, putative [Theobroma cacao]
          Length = 395

 Score =  178 bits (451), Expect = 5e-42
 Identities = 106/278 (38%), Positives = 155/278 (55%), Gaps = 13/278 (4%)
 Frame = +1

Query: 202 EFQVQHGDVLLASVLKSGTTWMKSLLHSIMACVHGFPNL-----LGDQNPHYIVPNIEM- 363
           +FQ Q  D+LL +  KSGTTW+K+++ ++M  V  +PN      L  +NPH +VP +E+ 
Sbjct: 127 QFQAQDTDILLVTTPKSGTTWLKAIVFALMNRVQ-YPNPEVKHPLLAENPHVLVPFLELE 185

Query: 364 ----SSVDSWPHLTSPRLLSTHLPLHALPRQIPHDDQLKIVYVTRNPRDTFVSFWKFWER 531
               + V  +   TSPRL  THLPL +LP  + +    K+VY+ RNP+DTFVS W F  +
Sbjct: 186 LYIDNRVADFTTFTSPRLFGTHLPLVSLPDSVKNS-ACKLVYLCRNPKDTFVSLWHFTNK 244

Query: 532 LAESAVKLGIQRHIPKEVLLEKFCAGHFQGGPFSDNVLSYLRESRRRPDKVLFVTYEDLH 711
           L    +          E   +KFC G    GPF D+VL Y +ES ++P++VLF+ YE++ 
Sbjct: 245 LRTKDMGEN-----SLEETFDKFCRGVCLYGPFWDHVLEYWKESLKKPERVLFLKYEEIK 299

Query: 712 ADCQAVICKIGNFLGYPSDVVE---GNVSQIAKRCSFRNISSLDINKKDESSEGRYPPNS 882
              +  + ++  FLG P    E   G V +I K CSF  +S+L++NK  +   G      
Sbjct: 300 EQPKVQLVRLAQFLGCPFSAQEETNGVVDEILKLCSFDTLSNLEVNKTGKLVSGE--EYK 357

Query: 883 IFFRSGETGGWREHFTPAMIERMRQEIEQKLEDEGLYF 996
            FFR GE G  + H TP M+E++ Q  + KL D GL F
Sbjct: 358 AFFRRGEVGDAKNHLTPQMMEKLDQITQDKLHDSGLIF 395


>ref|XP_002527846.1| Flavonol 3-sulfotransferase, putative [Ricinus communis]
           gi|223532770|gb|EEF34549.1| Flavonol 3-sulfotransferase,
           putative [Ricinus communis]
          Length = 333

 Score =  178 bits (451), Expect = 5e-42
 Identities = 113/296 (38%), Positives = 162/296 (54%), Gaps = 18/296 (6%)
 Frame = +1

Query: 166 FALPAFNAMHS--KEFQVQHGDVLLASVLKSGTTWMKSLLHSIM-ACVHGFPNL---LGD 327
           + L AF A+ S  K F     D+++AS+ KSGTTW+ SL++ I+   ++ F      L  
Sbjct: 41  YPLFAFKALISFQKHFHAHDKDIIVASMPKSGTTWLISLVYMIINRSLYAFKLNECPLLT 100

Query: 328 QNPHYIVPNIEMSSV--DSWPHLTS---PRLLSTHLPLHALPRQIPHDDQLKIVYVTRNP 492
            NPH ++P +E++    +  P L +   PR  +TH P  +LP  I  D   KIVYV RNP
Sbjct: 101 SNPHDLIPFLELNLYLKNQRPDLEAIPDPRTFTTHTPYSSLPTSII-DSNCKIVYVCRNP 159

Query: 493 RDTFVSFWKFWERLAESAVKLGIQRHIPKEVLLEKFCAGHFQG----GPFSDNVLSYLRE 660
            D F+S+W+F          L I+     +  LEK    H++G    GPF ++VL Y + 
Sbjct: 160 MDQFISYWRFL---------LKIKPITDDQTSLEKAFEMHYKGIHSFGPFCNHVLEYWKA 210

Query: 661 SRRRPDKVLFVTYEDLHADCQAVICKIGNFLGYP---SDVVEGNVSQIAKRCSFRNISSL 831
           S+  PDKVLF+ YE+L  D      K+  FLG+P    +  +G V +I + CSF N+ +L
Sbjct: 211 SQENPDKVLFLKYEELKEDIIGCTKKLAEFLGFPFSKDEEEQGIVEEITRICSFENLKNL 270

Query: 832 DINKKDESSEGRYPPNSIFFRSGETGGWREHFTPAMIERMRQEIEQKLEDEGLYFD 999
           D+NK  +   G   PN  FFR GE G W  H TP+M ERM   +++KL+  GL FD
Sbjct: 271 DVNKNGKRPSGA--PNDAFFRKGEVGDWSNHLTPSMAERMINLLQEKLKGSGLSFD 324


>gb|EAY97091.1| hypothetical protein OsI_19013 [Oryza sativa Indica Group]
          Length = 338

 Score =  178 bits (451), Expect = 5e-42
 Identities = 106/306 (34%), Positives = 160/306 (52%), Gaps = 12/306 (3%)
 Frame = +1

Query: 115 PMTKTIRLEQHN-TWIRDFALPAFNAMHSKEFQVQHGDVLLASVLKSGTTWMKSLLHSIM 291
           P    +RL  +   W+ ++ LP   A+       +HGDV+LAS  K GTTW+K+L  +++
Sbjct: 43  PSYPKLRLRHYQGMWLMEYTLPGIMAIQRSFVPRRHGDVVLASPGKCGTTWLKALAFAVL 102

Query: 292 ACVHGFP----NLLGDQNPHYIVPNIEMSSVDSW----PHLTSPRLLSTHLPLHALPRQI 447
           A     P    + L   NPH  VP +E +  + W      L SPRL+STH+   ALP+ I
Sbjct: 103 ARSAYSPASDRHPLLRLNPHDCVPFMEGAISEGWGGKIDELPSPRLMSTHMQHAALPKSI 162

Query: 448 PHDDQLKIVYVTRNPRDTFVSFWKFWERLAESAVKLGIQRHIPKEVLLEKFCAGHFQGGP 627
             +   K+VY+ R P+D  VS W F+           I+  +  + + E  C G F  G 
Sbjct: 163 ADEPGCKVVYICREPKDILVSAWHFFRI---------IEPDLSFQEVFEAACDGKFLTGA 213

Query: 628 FSDNVLSYLRESRRRPDKVLFVTYEDLHADCQAVICKIGNFLGYPSDVVE---GNVSQIA 798
             D+++ Y    +  P+KVLF+ YEDL  D   ++ K+ +FLG P    E   G V+ I 
Sbjct: 214 IWDHIIGYWNACKANPEKVLFLVYEDLLRDPANIVRKLADFLGQPFSSTEEESGLVTDIV 273

Query: 799 KRCSFRNISSLDINKKDESSEGRYPPNSIFFRSGETGGWREHFTPAMIERMRQEIEQKLE 978
           + CSF N+ SL++NK  E+S     PN+ +FR G+ G W+ H TP M+E     +++K+ 
Sbjct: 274 RLCSFENLKSLEVNKMGEASFAF--PNASYFRKGKAGDWKIHMTPEMVECFDTIVKEKMH 331

Query: 979 DEGLYF 996
             GL F
Sbjct: 332 GSGLVF 337


>ref|XP_006339394.1| PREDICTED: cytosolic sulfotransferase 12-like [Solanum tuberosum]
          Length = 328

 Score =  177 bits (450), Expect = 6e-42
 Identities = 103/282 (36%), Positives = 159/282 (56%), Gaps = 14/282 (4%)
 Frame = +1

Query: 199  KEFQVQHGDVLLASVLKSGTTWMKSLLHSIMACVH------GFPNLLGDQNPHYIVPNIE 360
            ++FQ Q  D++L +  KSG+TW+KSLL +++  V         P L+  +NPH +VP +E
Sbjct: 56   QQFQAQDSDIILVTTPKSGSTWLKSLLFALVNRVKHPIFEPSHPLLV--ENPHVLVPFLE 113

Query: 361  MS-----SVDSWPHLTSPRLLSTHLPLHALPRQIPHDDQLKIVYVTRNPRDTFVSFWKFW 525
             +      V  +   TSPRLL+TH+P  +LP+ +  D + K+VY+ RNPRDTF+S W F 
Sbjct: 114  HTLYIDGRVIDFSTFTSPRLLATHVPFASLPKSV-QDSKTKLVYLCRNPRDTFISMWHFA 172

Query: 526  ERLAESAVKLGIQRHIPKEVLLEKFCAGHFQGGPFSDNVLSYLRESRRRPDKVLFVTYED 705
              L      L  +     E + + FC G    GPF ++VL Y ++S   P+K+LF+ YE+
Sbjct: 173  NNLL-----LHHKDTNSIEEMFDLFCKGVSLYGPFWNHVLDYWKQSLENPNKILFLMYEE 227

Query: 706  LHADCQAVICKIGNFLGYPSDVVEGN---VSQIAKRCSFRNISSLDINKKDESSEGRYPP 876
            +    +  + ++  FL  P  + E N   V +I K CSF N+ +L++N   + S G   P
Sbjct: 228  IKKKPKIQLKRLAEFLECPFSIEEENSGVVDEILKMCSFENLRNLEVNTNGKFSTGE--P 285

Query: 877  NSIFFRSGETGGWREHFTPAMIERMRQEIEQKLEDEGLYFDY 1002
              +FFR GE G W+ +FT  MI+++   IE+K +  GL F Y
Sbjct: 286  YKVFFRRGEIGDWKNYFTIEMIDKLNHIIEEKFQGSGLKFSY 327


>ref|XP_004235426.1| PREDICTED: cytosolic sulfotransferase 5-like [Solanum lycopersicum]
          Length = 327

 Score =  177 bits (450), Expect = 6e-42
 Identities = 104/276 (37%), Positives = 153/276 (55%), Gaps = 8/276 (2%)
 Frame = +1

Query: 196 SKEFQVQHGDVLLASVLKSGTTWMKSLLHSIMACVHGFP---NLLGDQNPHYIVPNIEMS 366
           S  FQ  + DVLLAS  K+GTTW+K+L   I+   H  P   +LL   NP + V  +E S
Sbjct: 56  SSSFQAGNDDVLLASAPKTGTTWLKALCLCILHQNHNIPENEDLLTKDNPQFHVQTVESS 115

Query: 367 SVDSWP-----HLTSPRLLSTHLPLHALPRQIPHDDQLKIVYVTRNPRDTFVSFWKFWER 531
              + P        SPRL+ +HLP H LP  I + +  KIV V RNP+DT VS W F+  
Sbjct: 116 IYSTKPTPDLYSTPSPRLIHSHLPFHFLPNSIINSN-CKIVCVARNPKDTLVSLWHFF-- 172

Query: 532 LAESAVKLGIQRHIPKEVLLEKFCAGHFQGGPFSDNVLSYLRESRRRPDKVLFVTYEDLH 711
              +++  G Q   P E  +++FC G  Q GP+ +NVL Y  ES++RP+K+LF+ YE++ 
Sbjct: 173 ---NSIFRGNQEPYPLEKAVDEFCTGVHQYGPYFENVLGYWLESQKRPEKILFLKYEEMI 229

Query: 712 ADCQAVICKIGNFLGYPSDVVEGNVSQIAKRCSFRNISSLDINKKDESSEGRYPPNSIFF 891
            D +  + K+G FLG P +  E ++ ++  RCS   + +L++NK      G   PN+ +F
Sbjct: 230 KDPKEQVRKLGLFLGKPFE-KEEDLEKVVWRCSLERLRNLEVNKNGSVIYG--VPNASYF 286

Query: 892 RSGETGGWREHFTPAMIERMRQEIEQKLEDEGLYFD 999
           R G  G W+ +  P M  R+ Q    K +D  L F+
Sbjct: 287 RKGIVGDWKNYLNPEMENRINQTTHLKFKDSRLEFE 322


>gb|EOY01597.1| P-loop containing nucleoside triphosphate hydrolases superfamily
           protein [Theobroma cacao]
          Length = 331

 Score =  177 bits (448), Expect = 1e-41
 Identities = 105/286 (36%), Positives = 155/286 (54%), Gaps = 20/286 (6%)
 Frame = +1

Query: 199 KEFQVQHGDVLLASVLKSGTTWMKSLLHSIMACVHGFPNLLGD----QNPHYIV------ 348
           K FQ Q  D+ L +  KSGTTW+K+++ +++     +P+ +       NPH +V      
Sbjct: 62  KNFQAQDSDIFLTTTPKSGTTWLKAIVFALVNR-RKYPDPINHPLVTNNPHALVRYLELE 120

Query: 349 PNIEMSSVDSWPHLTSPRLLSTHLPLHALPRQIPHDDQLKIVYVTRNPRDTFVSFWKFWE 528
           P I+  + D+   + SPRL++THLP  +LP  + +    K+VY+ RNP+D FVSFW+F  
Sbjct: 121 PRIDYQAYDTSTTIASPRLIATHLPFVSLPESVKNS-ACKLVYLCRNPKDNFVSFWQFAN 179

Query: 529 RLAESAVKLGIQRHIPKEV-------LLEKFCAGHFQGGPFSDNVLSYLRESRRRPDKVL 687
            +            IPKE+        L+KFC G    GPF D+VL Y +ES   P K+L
Sbjct: 180 AV------------IPKEMGTNSLEETLDKFCRGGNIYGPFWDHVLGYWKESLENPQKLL 227

Query: 688 FVTYEDLHADCQAVICKIGNFLGYPSDVVE---GNVSQIAKRCSFRNISSLDINKKDESS 858
           F+ YE++    +  + +I  F+G P  + E   G V  I K CSF  +S+L++NK  + S
Sbjct: 228 FLKYEEMKEHPEIHLRRIAEFIGCPLSLEEESKGVVDDILKLCSFDTLSNLEVNKSGKLS 287

Query: 859 EGRYPPNSIFFRSGETGGWREHFTPAMIERMRQEIEQKLEDEGLYF 996
            G     S+FFR GE G W+ H TP MI ++ Q  E+K    GL F
Sbjct: 288 SGH--DTSVFFRRGEVGDWKNHLTPEMIRKLDQITEEKWRGSGLSF 331