BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Ephedra26_contig00005432
         (1405 letters)

Database: ./nr 
           37,332,560 sequences; 13,225,080,153 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_006664219.1| PREDICTED: putative folylpolyglutamate synth...   479   e-133
ref|XP_003535287.1| PREDICTED: folylpolyglutamate synthase, mito...   479   e-132
ref|XP_006490572.1| PREDICTED: folylpolyglutamate synthase, mito...   478   e-132
ref|XP_006422118.1| hypothetical protein CICLE_v10007115mg [Citr...   478   e-132
ref|XP_004308011.1| PREDICTED: folylpolyglutamate synthase-like ...   478   e-132
ref|XP_004958237.1| PREDICTED: folylpolyglutamate synthase, mito...   476   e-132
ref|XP_002267695.2| PREDICTED: folylpolyglutamate synthase-like ...   476   e-132
ref|XP_003544122.1| PREDICTED: LOW QUALITY PROTEIN: probable dih...   475   e-131
gb|ABA99426.2| dihydrofolate synthetase/folylpolyglutamate synth...   474   e-131
gb|ESW33330.1| hypothetical protein PHAVU_001G060700g [Phaseolus...   473   e-131
gb|ESW33328.1| hypothetical protein PHAVU_001G060700g [Phaseolus...   473   e-131
gb|EMJ22354.1| hypothetical protein PRUPE_ppa020012mg, partial [...   473   e-131
ref|XP_003577221.1| PREDICTED: folylpolyglutamate synthase-like ...   473   e-131
ref|XP_002442305.1| hypothetical protein SORBIDRAFT_08g017730 [S...   473   e-130
gb|AFW56474.1| GLA1 [Zea mays]                                        470   e-130
ref|NP_001151629.1| LOC100285263 [Zea mays] gi|195648214|gb|ACG4...   470   e-130
ref|XP_006374333.1| hypothetical protein POPTR_0015s06150g [Popu...   470   e-130
ref|XP_002331843.1| folylpolyglutamate synthase [Populus trichoc...   470   e-130
tpg|DAA55274.1| TPA: hypothetical protein ZEAMMB73_929164 [Zea m...   468   e-129
gb|EOY23454.1| Folylpolyglutamate synthetase family protein isof...   466   e-129

>ref|XP_006664219.1| PREDICTED: putative folylpolyglutamate synthase-like [Oryza
            brachyantha]
          Length = 511

 Score =  479 bits (1234), Expect = e-133
 Identities = 254/440 (57%), Positives = 319/440 (72%), Gaps = 3/440 (0%)
 Frame = -1

Query: 1345 MESLKNYEKEGIPKGAGTDSEEGFDLQRMERLIQLLGDPLSKYPVVHIAGTKGKGSTSAF 1166
            ME L+NYE+ G+P+GAGTDS++GFDL RM RL++ LGDP + +P VHIAGTKGKGST+AF
Sbjct: 1    MERLRNYERSGVPRGAGTDSDDGFDLGRMRRLLRCLGDPHTHFPAVHIAGTKGKGSTAAF 60

Query: 1165 ISNILREEGYSVGVYTSPHLKTIRERIVTGKKCEIMSADALKNLFDDVKHMLKDALEVEG 986
            +SNI+RE+GY+VG YTSPHL TIRERI  G+    +SA  L+N+F   K  +  ++E E 
Sbjct: 61   LSNIMREQGYNVGCYTSPHLLTIRERISVGESGGPVSAAVLRNIFGHAKEAIDQSIESED 120

Query: 985  LCLTHFEVFTALAFRFFADQNVDIAVIEAGLGGARDATNIIKSEGLLASVITTIGEEHLE 806
              LTHFEVFTAL++  F+ +NVDIA++EAGLGGARDATN+I+S  L ASVITTIG+EHL 
Sbjct: 121  GALTHFEVFTALSYLLFSRENVDIAIVEAGLGGARDATNVIQSTELAASVITTIGKEHLA 180

Query: 805  ALGGSLENIAVAKSGIIKQGRPVVLGSRFEFHIEQILRNRAALLNAPIISSCAPGIKS-- 632
            ALGGSL++IAVAKSGIIKQ RPV++G  F   IEQILR+RA L  +PIIS+C PG++S  
Sbjct: 181  ALGGSLQSIAVAKSGIIKQERPVIIGGPFSSDIEQILRDRAFLTQSPIISACDPGVRSIT 240

Query: 631  SISRLDCTGSIPYQYADISINLDEKNPNLMIDLAGVKIGMLGNHQLQNAITAICTSLCLR 452
               R DC    PYQ  DI I +    P L I+L  VKI +LG+HQ QNA+TA CT+LCLR
Sbjct: 241  RCIRWDC--GKPYQSCDIIIEISNNIP-LFIELHDVKIQLLGDHQRQNAVTACCTALCLR 297

Query: 451  EQGLRISDKSIITGLEKTSLLGRFQVLTPAEAESLGVVG-STIILDGAHTTASAKALADT 275
            E G  ISD SI  GLEKT L GR Q LT  E   LG+ G S++++DGAHT ASAKAL+D 
Sbjct: 298  ELGWDISDSSIRAGLEKTQLPGRSQFLTEEETSVLGLDGASSVLVDGAHTEASAKALSDV 357

Query: 274  IRRVYPDKPLAYVVSMANDKDHSGFARELISGTTPKVALFRDVSVAGSRSRTMPASLLTD 95
            I+ V P  PLA+VV MANDK+H  FA +L+SG  P + L  + S+AG RSR+MPAS L +
Sbjct: 358  IKTVKPRGPLAFVVGMANDKEHFAFAEQLLSGPRPDIVLLTEASIAGGRSRSMPASSLKE 417

Query: 94   TWTQTASEMMFSVTNCSNMT 35
             W   A     S ++   +T
Sbjct: 418  VWMTAAHSQGISYSDIGMIT 437


>ref|XP_003535287.1| PREDICTED: folylpolyglutamate synthase, mitochondrial-like isoform X1
            [Glycine max] gi|571481971|ref|XP_006588818.1| PREDICTED:
            folylpolyglutamate synthase, mitochondrial-like isoform
            X2 [Glycine max]
          Length = 536

 Score =  479 bits (1232), Expect = e-132
 Identities = 247/440 (56%), Positives = 319/440 (72%)
 Frame = -1

Query: 1369 KLQDLYNFMESLKNYEKEGIPKGAGTDSEEGFDLQRMERLIQLLGDPLSKYPVVHIAGTK 1190
            +++D  ++++SLKNYEK G+PKGAGTDS +GFDL RM RL++  G+P SK+  VHIAGTK
Sbjct: 25   EMKDFLDYLDSLKNYEKSGVPKGAGTDSSDGFDLGRMRRLMERFGNPHSKFKAVHIAGTK 84

Query: 1189 GKGSTSAFISNILREEGYSVGVYTSPHLKTIRERIVTGKKCEIMSADALKNLFDDVKHML 1010
            GKGST+AF+SNILR EGYSVG YTSPH+ TIRERI+ GK    +SA  L +LF  +K  +
Sbjct: 85   GKGSTAAFVSNILRNEGYSVGCYTSPHILTIRERILLGKSGHPVSAKLLNDLFRRIKPDI 144

Query: 1009 KDALEVEGLCLTHFEVFTALAFRFFADQNVDIAVIEAGLGGARDATNIIKSEGLLASVIT 830
              A++ E  C++HFEVFT +AF  FAD+NVDIAVIEAGLGGARDATNII S GL+ASVIT
Sbjct: 145  DQAMKEENGCISHFEVFTTMAFLLFADENVDIAVIEAGLGGARDATNIISSSGLVASVIT 204

Query: 829  TIGEEHLEALGGSLENIAVAKSGIIKQGRPVVLGSRFEFHIEQILRNRAALLNAPIISSC 650
            T+GEEHL ALGGSLE IA+AK+GIIKQ RP+VLG  F  HIE+I+R++A  +++P++S+ 
Sbjct: 205  TVGEEHLAALGGSLETIAMAKAGIIKQDRPLVLGGPFVPHIERIIRDKAVAMDSPVVSAY 264

Query: 649  APGIKSSISRLDCTGSIPYQYADISINLDEKNPNLMIDLAGVKIGMLGNHQLQNAITAIC 470
              G K ++         P Q  DI I +  K+  +   L  VK+ MLG+HQLQNA TA C
Sbjct: 265  DTGNKCTVKSFSIRNGRPCQICDILIQV-VKDMKMSCKLHDVKLQMLGDHQLQNAATATC 323

Query: 469  TSLCLREQGLRISDKSIITGLEKTSLLGRFQVLTPAEAESLGVVGSTIILDGAHTTASAK 290
             +L LR  G  ISD+SI  GLE T LLGR Q LT  EAE LG+ GST++LDGAHT  SAK
Sbjct: 324  VALVLRNLGWSISDESIRYGLEDTYLLGRSQFLTSEEAEVLGLTGSTVLLDGAHTKESAK 383

Query: 289  ALADTIRRVYPDKPLAYVVSMANDKDHSGFARELISGTTPKVALFRDVSVAGSRSRTMPA 110
            AL +TIR  +P   L +VV+MA+DKDH GFARE++SG   +  L  + ++AG  +RT PA
Sbjct: 384  ALMNTIRMAFPKAQLVFVVAMASDKDHVGFAREILSGVHVETVLLTEAAIAGGVTRTTPA 443

Query: 109  SLLTDTWTQTASEMMFSVTN 50
            SLL D+W + + E+   + +
Sbjct: 444  SLLRDSWIKASDELGIGIVH 463


>ref|XP_006490572.1| PREDICTED: folylpolyglutamate synthase, mitochondrial-like isoform X1
            [Citrus sinensis]
          Length = 556

 Score =  478 bits (1231), Expect = e-132
 Identities = 249/437 (56%), Positives = 318/437 (72%)
 Frame = -1

Query: 1378 EDEKLQDLYNFMESLKNYEKEGIPKGAGTDSEEGFDLQRMERLIQLLGDPLSKYPVVHIA 1199
            E+ +L +  N+++SLKN+EK G+PKGAGTDS++GFDL RM RL+  LG+P SK+  VHIA
Sbjct: 41   EEPELMNFMNYLDSLKNFEKSGVPKGAGTDSDDGFDLGRMNRLMDRLGNPHSKFKTVHIA 100

Query: 1198 GTKGKGSTSAFISNILREEGYSVGVYTSPHLKTIRERIVTGKKCEIMSADALKNLFDDVK 1019
            GTKGKGST+AF+S+ILR EGYSVG YTSPH+KTIRER+  G+    +SA AL  LF  +K
Sbjct: 101  GTKGKGSTAAFLSSILRAEGYSVGCYTSPHIKTIRERMNVGRLNRPVSAKALNCLFHKIK 160

Query: 1018 HMLKDALEVEGLCLTHFEVFTALAFRFFADQNVDIAVIEAGLGGARDATNIIKSEGLLAS 839
             +L +A+ +E  C+THFEV TA+AF  FA  +VDIAVIEAGLGGARDATNII S GL AS
Sbjct: 161  GVLDEAIRLENGCITHFEVLTAMAFALFAQNHVDIAVIEAGLGGARDATNIISSSGLAAS 220

Query: 838  VITTIGEEHLEALGGSLENIAVAKSGIIKQGRPVVLGSRFEFHIEQILRNRAALLNAPII 659
            VITTIGEEH  ALGGSLE IA+AKSGIIK GRP+VLG  F  HIE ILR+ A+L+ + ++
Sbjct: 221  VITTIGEEHTAALGGSLETIAMAKSGIIKYGRPLVLGGPFLPHIEHILRDEASLMCSQVV 280

Query: 658  SSCAPGIKSSISRLDCTGSIPYQYADISINLDEKNPNLMIDLAGVKIGMLGNHQLQNAIT 479
            S+   GI+++I+ L      P Q  DI +   E++  L I+L  VK+ M+GNHQL NA+T
Sbjct: 281  SAYDAGIRATINGLSMFNDRPCQSCDIIVQA-ERDLKLSIELLDVKLCMIGNHQLHNALT 339

Query: 478  AICTSLCLREQGLRISDKSIITGLEKTSLLGRFQVLTPAEAESLGVVGSTIILDGAHTTA 299
            A C +LCLR+QG +ISD S+  GLE T L+GR Q LT  +AE LG+ G+TI++DGAHT  
Sbjct: 340  ATCAALCLRDQGWKISDGSVRAGLENTCLVGRTQFLTSKQAELLGLPGATILVDGAHTKE 399

Query: 298  SAKALADTIRRVYPDKPLAYVVSMANDKDHSGFARELISGTTPKVALFRDVSVAGSRSRT 119
            SAKAL DTI+  +P   LA VV+MA DKDH  FA+E +SG      +  +  VAG ++R 
Sbjct: 400  SAKALVDTIQMTFPQARLALVVAMAIDKDHLAFAKEFLSGRKLDSVILTEADVAGGKARV 459

Query: 118  MPASLLTDTWTQTASEM 68
             PASLL D W Q + E+
Sbjct: 460  TPASLLRDRWIQASEEL 476


>ref|XP_006422118.1| hypothetical protein CICLE_v10007115mg [Citrus clementina]
            gi|557523991|gb|ESR35358.1| hypothetical protein
            CICLE_v10007115mg [Citrus clementina]
          Length = 565

 Score =  478 bits (1231), Expect = e-132
 Identities = 249/437 (56%), Positives = 318/437 (72%)
 Frame = -1

Query: 1378 EDEKLQDLYNFMESLKNYEKEGIPKGAGTDSEEGFDLQRMERLIQLLGDPLSKYPVVHIA 1199
            E+ +L +  N+++SLKN+EK G+PKGAGTDS++GFDL RM RL+  LG+P SK+  VHIA
Sbjct: 50   EEPELMNFMNYLDSLKNFEKSGVPKGAGTDSDDGFDLGRMNRLMDRLGNPHSKFKTVHIA 109

Query: 1198 GTKGKGSTSAFISNILREEGYSVGVYTSPHLKTIRERIVTGKKCEIMSADALKNLFDDVK 1019
            GTKGKGST+AF+S+ILR EGYSVG YTSPH+KTIRER+  G+    +SA AL  LF  +K
Sbjct: 110  GTKGKGSTAAFLSSILRAEGYSVGCYTSPHIKTIRERMNVGRLNRPVSAKALNCLFHKIK 169

Query: 1018 HMLKDALEVEGLCLTHFEVFTALAFRFFADQNVDIAVIEAGLGGARDATNIIKSEGLLAS 839
             +L +A+ +E  C+THFEV TA+AF  FA  +VDIAVIEAGLGGARDATNII S GL AS
Sbjct: 170  GVLDEAIRLENGCITHFEVLTAMAFALFAQNHVDIAVIEAGLGGARDATNIISSSGLAAS 229

Query: 838  VITTIGEEHLEALGGSLENIAVAKSGIIKQGRPVVLGSRFEFHIEQILRNRAALLNAPII 659
            VITTIGEEH  ALGGSLE IA+AKSGIIK GRP+VLG  F  HIE ILR+ A+L+ + ++
Sbjct: 230  VITTIGEEHTAALGGSLETIAMAKSGIIKYGRPLVLGGPFLPHIEHILRDEASLMCSQVV 289

Query: 658  SSCAPGIKSSISRLDCTGSIPYQYADISINLDEKNPNLMIDLAGVKIGMLGNHQLQNAIT 479
            S+   GI+++I+ L      P Q  DI +   E++  L I+L  VK+ M+GNHQL NA+T
Sbjct: 290  SAYDAGIRATINGLSMFNDRPCQSCDIIVQA-ERDLKLSIELLDVKLCMIGNHQLHNALT 348

Query: 478  AICTSLCLREQGLRISDKSIITGLEKTSLLGRFQVLTPAEAESLGVVGSTIILDGAHTTA 299
            A C +LCLR+QG +ISD S+  GLE T L+GR Q LT  +AE LG+ G+TI++DGAHT  
Sbjct: 349  ATCAALCLRDQGWKISDGSVRAGLENTCLVGRTQFLTSKQAELLGLPGATILVDGAHTKE 408

Query: 298  SAKALADTIRRVYPDKPLAYVVSMANDKDHSGFARELISGTTPKVALFRDVSVAGSRSRT 119
            SAKAL DTI+  +P   LA VV+MA DKDH  FA+E +SG      +  +  VAG ++R 
Sbjct: 409  SAKALVDTIQMTFPQARLALVVAMAIDKDHLAFAKEFLSGRKLDSVILTEADVAGGKARV 468

Query: 118  MPASLLTDTWTQTASEM 68
             PASLL D W Q + E+
Sbjct: 469  TPASLLRDRWIQASEEL 485


>ref|XP_004308011.1| PREDICTED: folylpolyglutamate synthase-like [Fragaria vesca subsp.
            vesca]
          Length = 553

 Score =  478 bits (1231), Expect = e-132
 Identities = 252/453 (55%), Positives = 325/453 (71%), Gaps = 5/453 (1%)
 Frame = -1

Query: 1378 EDEKLQDLYNFMESLKNYEKEGIPKGAGTDSEEGFDLQRMERLIQLLGDPLSKYPVVHIA 1199
            ED +L++L  +M+SLKNYEK G+P GAGTDS++GFDL RM RL++LLG P  K+  VHIA
Sbjct: 40   EDSELEELMEYMDSLKNYEKSGVPVGAGTDSDDGFDLGRMRRLMELLGSPQFKFKAVHIA 99

Query: 1198 GTKGKGSTSAFISNILREEGYSVGVYTSPHLKTIRERIVTGKKCEIMSADALKNLFDDVK 1019
            GTKGKGST+AF+ NILR EGYSVG YTSPH++TIRER+  G+  E +SA AL ++F + K
Sbjct: 100  GTKGKGSTAAFLCNILRAEGYSVGCYTSPHIRTIRERMTLGRSGEPVSAKALSSVFQENK 159

Query: 1018 HMLKDALEVEGLCLTHFEVFTALAFRFFADQNVDIAVIEAGLGGARDATNIIKSEGLLAS 839
              L  A+EVE  C++HFEV TALAF  FA + VDIAVIEAGLGGARDATN+I S  L  S
Sbjct: 160  ENLDRAIEVENGCISHFEVLTALAFTLFAKEKVDIAVIEAGLGGARDATNVIPSSALATS 219

Query: 838  VITTIGEEHLEALGGSLENIAVAKSGIIKQGRPVVLGSRFEFHIEQILRNRAALLNAPII 659
            +ITTIGEEHL ALGGSLE+IA+AKSGI+K G PVVLG  F  HIE ILR++A L ++P++
Sbjct: 220  IITTIGEEHLAALGGSLESIAMAKSGIMKHGCPVVLGGPFLPHIECILRDKALLTSSPVL 279

Query: 658  SSCAPGIKSSISRLDCTGSIPYQYADISINLDEKNPNLMIDLAGVKIGMLGNHQLQNAIT 479
             +     +S I         P+Q  DI I L E++  L ++L  +K+ MLG HQLQNA T
Sbjct: 280  LASDTRNRSMIHGFSMHNGRPFQSCDIVIQL-ERDIKLSVELLDLKLYMLGCHQLQNAAT 338

Query: 478  AICTSLCLREQGLRISDKSIITGLEKTSLLGRFQVLTPAEAESLGVVGSTIILDGAHTTA 299
            A C +LCLR  G RISD SI +GL++T LLGR Q LT  EAE+LG+ G+TI+LDGAHT  
Sbjct: 339  ATCAALCLRNLGWRISDASIRSGLQQTHLLGRSQFLTSEEAEALGLSGATILLDGAHTKE 398

Query: 298  SAKALADTIRRVYPDKPLAYVVSMANDKDHSGFARELISGTTPKVALFRDVSVAGSRSRT 119
            SAKAL DTI+ ++P+  L  VV+MANDKDH GFARE +SG   +  +  + ++AG +SRT
Sbjct: 399  SAKALMDTIKMIFPESRLTVVVAMANDKDHLGFAREFLSGGHLEGVICTEANIAGGKSRT 458

Query: 118  MPASLLTDTWTQTASEMMFSV-----TNCSNMT 35
              AS+L + W Q + E+   +     T+C + +
Sbjct: 459  TAASMLRNCWIQASKELGLDIDHDQMTDCQDFS 491


>ref|XP_004958237.1| PREDICTED: folylpolyglutamate synthase, mitochondrial-like [Setaria
            italica]
          Length = 559

 Score =  476 bits (1226), Expect = e-132
 Identities = 247/453 (54%), Positives = 322/453 (71%), Gaps = 1/453 (0%)
 Frame = -1

Query: 1369 KLQDLYNFMESLKNYEKEGIPKGAGTDSEEGFDLQRMERLIQLLGDPLSKYPVVHIAGTK 1190
            +L D + +ME L+NYE+ G+P+GAGTDS++GFDL RM RL++ LGDP + +P VHIAGTK
Sbjct: 36   QLGDFFEYMERLRNYERSGVPRGAGTDSDDGFDLGRMRRLLRRLGDPHTHFPAVHIAGTK 95

Query: 1189 GKGSTSAFISNILREEGYSVGVYTSPHLKTIRERIVTGKKCEIMSADALKNLFDDVKHML 1010
            GKGST+AF+SNI+RE+GY+VG Y+SPHL TIRERI  G     +    L +LFD  K  +
Sbjct: 96   GKGSTAAFLSNIMREQGYNVGCYSSPHLLTIRERISVGNDGGPVPVGLLSDLFDQAKEAI 155

Query: 1009 KDALEVEGLCLTHFEVFTALAFRFFADQNVDIAVIEAGLGGARDATNIIKSEGLLASVIT 830
             +++E+E   LTHFEVFTAL+F  F+ +NVDIA++EAGLGGARDATN+I+S  L ASVIT
Sbjct: 156  DESIELENGALTHFEVFTALSFLLFSQENVDIAIVEAGLGGARDATNVIQSTELAASVIT 215

Query: 829  TIGEEHLEALGGSLENIAVAKSGIIKQGRPVVLGSRFEFHIEQILRNRAALLNAPIISSC 650
            T+G EHL ALGGSL+NIA+AKSGIIKQGRPVV+G  F   IEQI+R+RA L  +P+IS+C
Sbjct: 216  TVGREHLAALGGSLQNIAIAKSGIIKQGRPVVIGGPFSTDIEQIIRDRAFLTQSPVISAC 275

Query: 649  APGIKSSISRLDCTGSIPYQYADISINLDEKNPNLMIDLAGVKIGMLGNHQLQNAITAIC 470
             PGIKS    +      PYQ  DISI +   +    I+L  V + +LG+HQ QNA+TA C
Sbjct: 276  DPGIKSISKCIYWDNGKPYQRCDISIKI-SNDTLFSIELHDVNLQLLGDHQRQNAVTASC 334

Query: 469  TSLCLREQGLRISDKSIITGLEKTSLLGRFQVLTPAEAESLGVVG-STIILDGAHTTASA 293
            T+LCLR  G  ISD SI +GLE+T L GR Q LT  EA  LG+ G STI++DGAHT ASA
Sbjct: 335  TALCLRNLGWDISDASIQSGLEETQLPGRSQFLTREEALVLGLDGPSTILIDGAHTEASA 394

Query: 292  KALADTIRRVYPDKPLAYVVSMANDKDHSGFARELISGTTPKVALFRDVSVAGSRSRTMP 113
            KAL++ I  V P+ PLA VV MANDK+H  FA +L++G+ P V L  + S+AG  SR MP
Sbjct: 395  KALSNVIETVRPEGPLALVVGMANDKEHLAFAEQLLAGSRPNVVLLTEASIAGGASRAMP 454

Query: 112  ASLLTDTWTQTASEMMFSVTNCSNMTRKEPNKH 14
            A  L + W   A ++  +  +   ++  +  +H
Sbjct: 455  APSLKEIWMAAARDLGINCVDIGTISGAKAPEH 487


>ref|XP_002267695.2| PREDICTED: folylpolyglutamate synthase-like [Vitis vinifera]
            gi|302143688|emb|CBI22549.3| unnamed protein product
            [Vitis vinifera]
          Length = 536

 Score =  476 bits (1226), Expect = e-132
 Identities = 247/436 (56%), Positives = 324/436 (74%)
 Frame = -1

Query: 1375 DEKLQDLYNFMESLKNYEKEGIPKGAGTDSEEGFDLQRMERLIQLLGDPLSKYPVVHIAG 1196
            + +L+D  N++++LKNYEK G+PK AGTDS  GFDL RM RL+  LG+P + +  VHIAG
Sbjct: 23   EPELKDFLNYLDNLKNYEKCGVPKDAGTDSSHGFDLGRMNRLMDRLGNPETGFKAVHIAG 82

Query: 1195 TKGKGSTSAFISNILREEGYSVGVYTSPHLKTIRERIVTGKKCEIMSADALKNLFDDVKH 1016
            TKGKGST+AF++NILR EGY+VG YTSPH++TIRERI  GK  E +SA AL  LF  +K 
Sbjct: 83   TKGKGSTAAFLANILRTEGYAVGCYTSPHVRTIRERISLGKLGEPVSAKALNCLFHRIKP 142

Query: 1015 MLKDALEVEGLCLTHFEVFTALAFRFFADQNVDIAVIEAGLGGARDATNIIKSEGLLASV 836
            +L +A+ +E   L+HFE+ TA+AF+ FA +NVD+AVIEAGLGGARDATNII S GL A+V
Sbjct: 143  ILDEAVALENGRLSHFEILTAMAFKLFAQENVDVAVIEAGLGGARDATNIISSSGLAAAV 202

Query: 835  ITTIGEEHLEALGGSLENIAVAKSGIIKQGRPVVLGSRFEFHIEQILRNRAALLNAPIIS 656
            ITTIGEEHL ALGGSLE+IA+AKSGIIKQG P+VLG  F  HIE I  ++A+ + +P++S
Sbjct: 203  ITTIGEEHLAALGGSLESIAMAKSGIIKQGCPLVLGGPFLPHIEHIFLDKASSMCSPVVS 262

Query: 655  SCAPGIKSSISRLDCTGSIPYQYADISINLDEKNPNLMIDLAGVKIGMLGNHQLQNAITA 476
            +  PG +S++  +  +   P+Q  DI I + E++  L I+L  VK+ MLG HQLQNA TA
Sbjct: 263  ASGPGNRSAVKGVSKSNGKPFQSCDIVIEV-ERDFKLFIELFDVKLQMLGIHQLQNAATA 321

Query: 475  ICTSLCLREQGLRISDKSIITGLEKTSLLGRFQVLTPAEAESLGVVGSTIILDGAHTTAS 296
             C +LCLR++G RISD+SI  GLE   LLGR Q LT  EAE+LG+ G+TI+LDGAHT  S
Sbjct: 322  TCVALCLRDKGWRISDESIHAGLEHAYLLGRSQFLTSTEAETLGLPGATIMLDGAHTKES 381

Query: 295  AKALADTIRRVYPDKPLAYVVSMANDKDHSGFARELISGTTPKVALFRDVSVAGSRSRTM 116
            AKAL DTI+  +P+  LA +V+MA+DKDH  FARE +SG   +     +V++AG++SRT 
Sbjct: 382  AKALVDTIQMTFPEARLALIVAMASDKDHMAFAREFLSGGQLEAVFLTEVNIAGAKSRTT 441

Query: 115  PASLLTDTWTQTASEM 68
             AS+L D W Q + E+
Sbjct: 442  SASMLRDCWIQASKEL 457


>ref|XP_003544122.1| PREDICTED: LOW QUALITY PROTEIN: probable dihydrofolate
            synthetase-like [Glycine max]
          Length = 536

 Score =  475 bits (1223), Expect = e-131
 Identities = 245/440 (55%), Positives = 317/440 (72%)
 Frame = -1

Query: 1369 KLQDLYNFMESLKNYEKEGIPKGAGTDSEEGFDLQRMERLIQLLGDPLSKYPVVHIAGTK 1190
            +++D  ++++SLKNYEK G+PKGAGTDS +GFDL RM  L++  G+P SK+  VHIAGTK
Sbjct: 25   EMKDFLDYLDSLKNYEKSGVPKGAGTDSSDGFDLGRMRCLMERFGNPHSKFKAVHIAGTK 84

Query: 1189 GKGSTSAFISNILREEGYSVGVYTSPHLKTIRERIVTGKKCEIMSADALKNLFDDVKHML 1010
            GK ST+AF+SNILR EGYSVG YTSPH+ TIRERI+ GK   ++SA  L +LF  +K  +
Sbjct: 85   GKASTAAFVSNILRNEGYSVGCYTSPHILTIRERILLGKSGHLVSAKLLNDLFRRIKPDI 144

Query: 1009 KDALEVEGLCLTHFEVFTALAFRFFADQNVDIAVIEAGLGGARDATNIIKSEGLLASVIT 830
              A++ E  C++HFEVFT +AF  FAD+NVD AVIEAGLGGARDATNII S GL+ASVIT
Sbjct: 145  DQAMKEENGCISHFEVFTTMAFLLFADENVDFAVIEAGLGGARDATNIISSSGLVASVIT 204

Query: 829  TIGEEHLEALGGSLENIAVAKSGIIKQGRPVVLGSRFEFHIEQILRNRAALLNAPIISSC 650
            T+GEEHL ALGGSLE IA+AK+GIIKQ RP+VLG  F  HIE+I+R++A  +N+P++S+ 
Sbjct: 205  TVGEEHLAALGGSLETIAMAKAGIIKQDRPLVLGGPFVPHIERIIRDKAVAMNSPVVSAY 264

Query: 649  APGIKSSISRLDCTGSIPYQYADISINLDEKNPNLMIDLAGVKIGMLGNHQLQNAITAIC 470
              G K ++         P Q  DI I +  K+  +   L  VK+ MLG+HQLQNA TA C
Sbjct: 265  DTGNKCTMKSFSILNGRPCQICDILIQV-VKDMKMSCKLHDVKLQMLGDHQLQNAATATC 323

Query: 469  TSLCLREQGLRISDKSIITGLEKTSLLGRFQVLTPAEAESLGVVGSTIILDGAHTTASAK 290
             +L LR  G  ISDKSI  GLE T LLGR Q LT  EAE LG+ GST++LDGAHT  SAK
Sbjct: 324  VALVLRNLGWSISDKSIRYGLEDTYLLGRSQFLTSEEAEVLGLTGSTVLLDGAHTKESAK 383

Query: 289  ALADTIRRVYPDKPLAYVVSMANDKDHSGFARELISGTTPKVALFRDVSVAGSRSRTMPA 110
            AL +TIR  +P   L +VV+MA+DKDH GFARE++SG   +  +  + ++AG  +RT PA
Sbjct: 384  ALMNTIRMAFPKAQLVFVVAMASDKDHVGFAREILSGVHVETVVLTEAAIAGGVTRTTPA 443

Query: 109  SLLTDTWTQTASEMMFSVTN 50
            SLL D+W + + E+   + +
Sbjct: 444  SLLRDSWIKASGELGIGIVH 463


>gb|ABA99426.2| dihydrofolate synthetase/folylpolyglutamate synthetase, putative,
            expressed [Oryza sativa Japonica Group]
            gi|218187281|gb|EEC69708.1| hypothetical protein
            OsI_39178 [Oryza sativa Indica Group]
            gi|222617510|gb|EEE53642.1| hypothetical protein
            OsJ_36925 [Oryza sativa Japonica Group]
          Length = 510

 Score =  474 bits (1221), Expect = e-131
 Identities = 248/430 (57%), Positives = 313/430 (72%), Gaps = 1/430 (0%)
 Frame = -1

Query: 1345 MESLKNYEKEGIPKGAGTDSEEGFDLQRMERLIQLLGDPLSKYPVVHIAGTKGKGSTSAF 1166
            ME L+NYE+ G+P+GAGTDS++GFDL RM RL++ LGDP + +P VHIAGTKGKGST+AF
Sbjct: 1    MERLRNYERSGVPRGAGTDSDDGFDLGRMRRLLRRLGDPHTHFPAVHIAGTKGKGSTAAF 60

Query: 1165 ISNILREEGYSVGVYTSPHLKTIRERIVTGKKCEIMSADALKNLFDDVKHMLKDALEVEG 986
            +SNI+RE+GY+VG YTSPHL TIRERI  G+    +SA+ L++LF   K  +  ++E E 
Sbjct: 61   LSNIMREQGYNVGCYTSPHLLTIRERISVGQSGGPVSAELLRDLFGHAKEAIGQSIESED 120

Query: 985  LCLTHFEVFTALAFRFFADQNVDIAVIEAGLGGARDATNIIKSEGLLASVITTIGEEHLE 806
              LTHFEVFTAL++  F+ +NVDIA++EAGLGGARDATN+I+S  L ASVITT+G+EHL 
Sbjct: 121  GALTHFEVFTALSYLLFSQENVDIAIVEAGLGGARDATNVIQSTELAASVITTVGKEHLA 180

Query: 805  ALGGSLENIAVAKSGIIKQGRPVVLGSRFEFHIEQILRNRAALLNAPIISSCAPGIKSSI 626
            ALGGSL++IAVAKSGIIKQ RPV++G  F   IEQILR+RA L  +P+IS+C PGI+S  
Sbjct: 181  ALGGSLQSIAVAKSGIIKQERPVIIGGPFSPDIEQILRDRAFLTQSPVISACDPGIRSIT 240

Query: 625  SRLDCTGSIPYQYADISINLDEKNPNLMIDLAGVKIGMLGNHQLQNAITAICTSLCLREQ 446
              +      PYQ  DI I +    P L I+L  +K+ +LG+HQ QNA+TA CT+LCLRE 
Sbjct: 241  RCIGWDCGKPYQSCDIVIEISNDIP-LFIELQDLKLQLLGDHQRQNAVTACCTALCLREL 299

Query: 445  GLRISDKSIITGLEKTSLLGRFQVLTPAEAESLGVVG-STIILDGAHTTASAKALADTIR 269
            G  ISD SI  GLEKT L GR Q LT  E   LG+ G S++++DGAHT ASAKAL+D I+
Sbjct: 300  GWNISDTSIQAGLEKTQLPGRSQFLTKEETSVLGLDGTSSVLVDGAHTEASAKALSDVIK 359

Query: 268  RVYPDKPLAYVVSMANDKDHSGFARELISGTTPKVALFRDVSVAGSRSRTMPASLLTDTW 89
             V P  PLA VV MANDK+H  FA +L+SG  P V L  + S+AG RSR+MPAS L + W
Sbjct: 360  TVQPRGPLALVVGMANDKEHLAFAEQLLSGPRPDVVLLTEASIAGGRSRSMPASSLKEIW 419

Query: 88   TQTASEMMFS 59
               A     S
Sbjct: 420  MAAAQSQGIS 429


>gb|ESW33330.1| hypothetical protein PHAVU_001G060700g [Phaseolus vulgaris]
          Length = 548

 Score =  473 bits (1218), Expect = e-131
 Identities = 242/441 (54%), Positives = 321/441 (72%)
 Frame = -1

Query: 1390 KLVMEDEKLQDLYNFMESLKNYEKEGIPKGAGTDSEEGFDLQRMERLIQLLGDPLSKYPV 1211
            + +  + +++DL ++++SLKN+EK G+P+GAGTDS+EGFDL RM RL++  G+P + +  
Sbjct: 31   RTLSSNPEMKDLSDYLDSLKNFEKSGLPRGAGTDSDEGFDLGRMRRLMERFGNPHTNFKA 90

Query: 1210 VHIAGTKGKGSTSAFISNILREEGYSVGVYTSPHLKTIRERIVTGKKCEIMSADALKNLF 1031
            VHIAGTKGKGST+AFISNILR EGYSVG YTSPH+ TIRERI+ G+    +S   L +LF
Sbjct: 91   VHIAGTKGKGSTAAFISNILRTEGYSVGCYTSPHILTIRERILLGRSGHPVSGKLLNDLF 150

Query: 1030 DDVKHMLKDALEVEGLCLTHFEVFTALAFRFFADQNVDIAVIEAGLGGARDATNIIKSEG 851
              +K  L+ A++ E  C++HFEVFTA+AF  FAD+NVDIAVIEAGLGGARDATNII S G
Sbjct: 151  HRIKPKLEQAMKEENGCISHFEVFTAMAFVLFADENVDIAVIEAGLGGARDATNIISSSG 210

Query: 850  LLASVITTIGEEHLEALGGSLENIAVAKSGIIKQGRPVVLGSRFEFHIEQILRNRAALLN 671
            L A+VITT+GEEHL ALGGSLE IA+AK+GIIKQG P+VLG  F  HIE+I+R++AA + 
Sbjct: 211  LAAAVITTVGEEHLAALGGSLETIAMAKAGIIKQGCPLVLGGPFVPHIERIIRDKAATME 270

Query: 670  APIISSCAPGIKSSISRLDCTGSIPYQYADISINLDEKNPNLMIDLAGVKIGMLGNHQLQ 491
            +P++S+   G + ++         PYQ  DI I +  K+  +   L  VK+ MLG+HQLQ
Sbjct: 271  SPVVSAYDTGNRCTLKSFSIVNGRPYQICDIVIQV-VKDLKMSCKLHDVKLKMLGDHQLQ 329

Query: 490  NAITAICTSLCLREQGLRISDKSIITGLEKTSLLGRFQVLTPAEAESLGVVGSTIILDGA 311
            NA TA C +LCLR  G  ISD+SI +GLE T LLGR Q LT  EA  LG+ G+T++LDGA
Sbjct: 330  NAATATCVALCLRNLGWSISDESIRSGLEHTHLLGRSQFLTSEEAGVLGLTGATVLLDGA 389

Query: 310  HTTASAKALADTIRRVYPDKPLAYVVSMANDKDHSGFARELISGTTPKVALFRDVSVAGS 131
            HT  SAKAL  T++  +P  PL +VV+MA+DKDH GFARE++ G   +  L  + ++AG 
Sbjct: 390  HTKESAKALMKTVKMTFPKAPLIFVVAMASDKDHVGFAREILLGGHVETVLLTEAAIAGG 449

Query: 130  RSRTMPASLLTDTWTQTASEM 68
             +RT  ASLL D+W + + E+
Sbjct: 450  VTRTTSASLLRDSWIKASDEL 470


>gb|ESW33328.1| hypothetical protein PHAVU_001G060700g [Phaseolus vulgaris]
          Length = 535

 Score =  473 bits (1218), Expect = e-131
 Identities = 242/441 (54%), Positives = 321/441 (72%)
 Frame = -1

Query: 1390 KLVMEDEKLQDLYNFMESLKNYEKEGIPKGAGTDSEEGFDLQRMERLIQLLGDPLSKYPV 1211
            + +  + +++DL ++++SLKN+EK G+P+GAGTDS+EGFDL RM RL++  G+P + +  
Sbjct: 18   RTLSSNPEMKDLSDYLDSLKNFEKSGLPRGAGTDSDEGFDLGRMRRLMERFGNPHTNFKA 77

Query: 1210 VHIAGTKGKGSTSAFISNILREEGYSVGVYTSPHLKTIRERIVTGKKCEIMSADALKNLF 1031
            VHIAGTKGKGST+AFISNILR EGYSVG YTSPH+ TIRERI+ G+    +S   L +LF
Sbjct: 78   VHIAGTKGKGSTAAFISNILRTEGYSVGCYTSPHILTIRERILLGRSGHPVSGKLLNDLF 137

Query: 1030 DDVKHMLKDALEVEGLCLTHFEVFTALAFRFFADQNVDIAVIEAGLGGARDATNIIKSEG 851
              +K  L+ A++ E  C++HFEVFTA+AF  FAD+NVDIAVIEAGLGGARDATNII S G
Sbjct: 138  HRIKPKLEQAMKEENGCISHFEVFTAMAFVLFADENVDIAVIEAGLGGARDATNIISSSG 197

Query: 850  LLASVITTIGEEHLEALGGSLENIAVAKSGIIKQGRPVVLGSRFEFHIEQILRNRAALLN 671
            L A+VITT+GEEHL ALGGSLE IA+AK+GIIKQG P+VLG  F  HIE+I+R++AA + 
Sbjct: 198  LAAAVITTVGEEHLAALGGSLETIAMAKAGIIKQGCPLVLGGPFVPHIERIIRDKAATME 257

Query: 670  APIISSCAPGIKSSISRLDCTGSIPYQYADISINLDEKNPNLMIDLAGVKIGMLGNHQLQ 491
            +P++S+   G + ++         PYQ  DI I +  K+  +   L  VK+ MLG+HQLQ
Sbjct: 258  SPVVSAYDTGNRCTLKSFSIVNGRPYQICDIVIQV-VKDLKMSCKLHDVKLKMLGDHQLQ 316

Query: 490  NAITAICTSLCLREQGLRISDKSIITGLEKTSLLGRFQVLTPAEAESLGVVGSTIILDGA 311
            NA TA C +LCLR  G  ISD+SI +GLE T LLGR Q LT  EA  LG+ G+T++LDGA
Sbjct: 317  NAATATCVALCLRNLGWSISDESIRSGLEHTHLLGRSQFLTSEEAGVLGLTGATVLLDGA 376

Query: 310  HTTASAKALADTIRRVYPDKPLAYVVSMANDKDHSGFARELISGTTPKVALFRDVSVAGS 131
            HT  SAKAL  T++  +P  PL +VV+MA+DKDH GFARE++ G   +  L  + ++AG 
Sbjct: 377  HTKESAKALMKTVKMTFPKAPLIFVVAMASDKDHVGFAREILLGGHVETVLLTEAAIAGG 436

Query: 130  RSRTMPASLLTDTWTQTASEM 68
             +RT  ASLL D+W + + E+
Sbjct: 437  VTRTTSASLLRDSWIKASDEL 457


>gb|EMJ22354.1| hypothetical protein PRUPE_ppa020012mg, partial [Prunus persica]
          Length = 499

 Score =  473 bits (1218), Expect = e-131
 Identities = 250/431 (58%), Positives = 319/431 (74%)
 Frame = -1

Query: 1348 FMESLKNYEKEGIPKGAGTDSEEGFDLQRMERLIQLLGDPLSKYPVVHIAGTKGKGSTSA 1169
            +M SLKNYEK G+PK AGTDSE+GFDL RM RL++LLG+P SK+  VHIAGTKGKGST+A
Sbjct: 3    YMNSLKNYEKSGVPKDAGTDSEDGFDLGRMRRLLELLGNPHSKFKAVHIAGTKGKGSTAA 62

Query: 1168 FISNILREEGYSVGVYTSPHLKTIRERIVTGKKCEIMSADALKNLFDDVKHMLKDALEVE 989
            F+S+ILR EG+SVG YTSPH++TIRERI  G+  E +SA AL++LF  +K +L  A+EVE
Sbjct: 63   FLSSILRAEGHSVGCYTSPHIQTIRERISMGRFGEPVSAKALRSLFHRIKKILDRAMEVE 122

Query: 988  GLCLTHFEVFTALAFRFFADQNVDIAVIEAGLGGARDATNIIKSEGLLASVITTIGEEHL 809
               ++HFEV TA+AF  FA +NV IAVIEAGLGGARDATN+I S GL  SVITTIGEEHL
Sbjct: 123  NGRMSHFEVLTAMAFTLFAQENVGIAVIEAGLGGARDATNVICSSGLATSVITTIGEEHL 182

Query: 808  EALGGSLENIAVAKSGIIKQGRPVVLGSRFEFHIEQILRNRAALLNAPIISSCAPGIKSS 629
             ALGGSLE+IA AKSGIIK GRPVVLG  F  HIE ILR++A L+++P++ +   G +S 
Sbjct: 183  AALGGSLESIATAKSGIIKHGRPVVLGGPFLPHIEHILRDKAFLMSSPVVLASDAGNRSK 242

Query: 628  ISRLDCTGSIPYQYADISINLDEKNPNLMIDLAGVKIGMLGNHQLQNAITAICTSLCLRE 449
            I+ +      P+Q  DI I L E++  L I+L  VK+ MLG HQLQNA+TA C +LCLR 
Sbjct: 243  INGVSMLNGRPFQSCDIVIQL-ERDLKLFIELLDVKLYMLGTHQLQNAVTATCAALCLRN 301

Query: 448  QGLRISDKSIITGLEKTSLLGRFQVLTPAEAESLGVVGSTIILDGAHTTASAKALADTIR 269
             G RISD+ I TGL++T LLGR Q LT  EA+++ +  +TI++DGAHT  SA+AL +TI+
Sbjct: 302  LGWRISDECIRTGLQQTHLLGRSQFLTSKEAQAIRLSDATILVDGAHTKESARALMETIK 361

Query: 268  RVYPDKPLAYVVSMANDKDHSGFARELISGTTPKVALFRDVSVAGSRSRTMPASLLTDTW 89
              +P+  L  VV+MA+DKDH GFARE +SG      +  + S+AGS+SRT  ASLL + W
Sbjct: 362  MTFPEAGLTLVVAMASDKDHLGFARECLSGGHVDGVVLTEASIAGSKSRTTAASLLRNCW 421

Query: 88   TQTASEMMFSV 56
             Q + E+   V
Sbjct: 422  IQASKELGIDV 432


>ref|XP_003577221.1| PREDICTED: folylpolyglutamate synthase-like [Brachypodium distachyon]
          Length = 563

 Score =  473 bits (1218), Expect = e-131
 Identities = 244/452 (53%), Positives = 321/452 (71%), Gaps = 1/452 (0%)
 Frame = -1

Query: 1378 EDEKLQDLYNFMESLKNYEKEGIPKGAGTDSEEGFDLQRMERLIQLLGDPLSKYPVVHIA 1199
            E+ KL +   +M+ L+NYE+ G+P+GAGTDS++GFDL RM RL++ LGDP + YP VHIA
Sbjct: 36   EEGKLGEFLEYMDRLRNYERSGVPRGAGTDSDDGFDLGRMRRLLRRLGDPHTHYPAVHIA 95

Query: 1198 GTKGKGSTSAFISNILREEGYSVGVYTSPHLKTIRERIVTGKKCEIMSADALKNLFDDVK 1019
            GTKGKGST+AF+SNILRE+GY+VG Y+SPHL TIRERI  G+    + A  L +LFD  K
Sbjct: 96   GTKGKGSTAAFLSNILREQGYNVGCYSSPHLLTIRERISVGEHGGPVPAALLSDLFDQAK 155

Query: 1018 HMLKDALEVEGLCLTHFEVFTALAFRFFADQNVDIAVIEAGLGGARDATNIIKSEGLLAS 839
              +  ++E E   LTHFEVFT L+F  F+ +NVD A++EAGLGGARDATN+I++  L AS
Sbjct: 156  EAIDQSIESESGALTHFEVFTTLSFLLFSRENVDFAIVEAGLGGARDATNVIRNTELAAS 215

Query: 838  VITTIGEEHLEALGGSLENIAVAKSGIIKQGRPVVLGSRFEFHIEQILRNRAALLNAPII 659
            VITT+G+EHL ALGGSL++IAVAKSGIIK+G+PV++G  F   IEQI+R+RA L  + +I
Sbjct: 216  VITTVGKEHLAALGGSLQSIAVAKSGIIKEGQPVIIGGPFSTDIEQIIRDRAFLTQSTVI 275

Query: 658  SSCAPGIKSSISRLDCTGSIPYQYADISINLDEKNPNLMIDLAGVKIGMLGNHQLQNAIT 479
            S+C PG++S    +      PYQ  DI++ +    P L ++L  +K+ +LG+HQ QNA+T
Sbjct: 276  SACDPGVRSITRCVGWDYGKPYQSCDIAVQISNDMP-LSMELHDIKLQLLGDHQRQNAVT 334

Query: 478  AICTSLCLREQGLRISDKSIITGLEKTSLLGRFQVLTPAEAESLGV-VGSTIILDGAHTT 302
            A CT+LCLRE G  ISD SI  GLEKT LLGR Q LT  EA  LG+   STI++DGAHT 
Sbjct: 335  ASCTALCLRELGWNISDASIRAGLEKTQLLGRSQFLTHDEASVLGLDRSSTILIDGAHTE 394

Query: 301  ASAKALADTIRRVYPDKPLAYVVSMANDKDHSGFARELISGTTPKVALFRDVSVAGSRSR 122
            ASAKAL+D IR V P+ PLA ++ MANDK+H  FA +L+SG  P + L  +  +AG  +R
Sbjct: 395  ASAKALSDVIRTVEPEGPLALIIGMANDKEHLAFAAQLLSGRRPDIVLLTEARIAGGTAR 454

Query: 121  TMPASLLTDTWTQTASEMMFSVTNCSNMTRKE 26
            +M AS L D WT  A +     T+   +T  E
Sbjct: 455  SMLASSLKDIWTGVAHDQGIDCTDIGVVTSDE 486


>ref|XP_002442305.1| hypothetical protein SORBIDRAFT_08g017730 [Sorghum bicolor]
            gi|241942998|gb|EES16143.1| hypothetical protein
            SORBIDRAFT_08g017730 [Sorghum bicolor]
          Length = 567

 Score =  473 bits (1216), Expect = e-130
 Identities = 245/456 (53%), Positives = 317/456 (69%), Gaps = 1/456 (0%)
 Frame = -1

Query: 1378 EDEKLQDLYNFMESLKNYEKEGIPKGAGTDSEEGFDLQRMERLIQLLGDPLSKYPVVHIA 1199
            E+  L     +ME L+NYE+ G+P+GAGTDS++GFDL RM RL++ LGDP + +P VHIA
Sbjct: 42   EEGPLGGFLEYMERLRNYERSGVPRGAGTDSDDGFDLGRMRRLLRRLGDPHTHFPAVHIA 101

Query: 1198 GTKGKGSTSAFISNILREEGYSVGVYTSPHLKTIRERIVTGKKCEIMSADALKNLFDDVK 1019
            GTKGKGST+AF+SNI+R +GY+VG Y+SPHL TIRERI  G     +    L +LFD+ K
Sbjct: 102  GTKGKGSTAAFLSNIMRAQGYNVGCYSSPHLLTIRERISVGNDGGPVPVGLLSDLFDEAK 161

Query: 1018 HMLKDALEVEGLCLTHFEVFTALAFRFFADQNVDIAVIEAGLGGARDATNIIKSEGLLAS 839
              + +A+E E   LTHFEVFTAL+F  F+ +NVDIA+IEAGLGGARDATN+I+S  L AS
Sbjct: 162  EAIDEAIESENGSLTHFEVFTALSFLLFSQENVDIAIIEAGLGGARDATNVIRSTELAAS 221

Query: 838  VITTIGEEHLEALGGSLENIAVAKSGIIKQGRPVVLGSRFEFHIEQILRNRAALLNAPII 659
            VITT+G EHL ALGGSL++IA+AKSGIIKQGRPVV+G  F   IEQI+R+RA L  +P+I
Sbjct: 222  VITTVGREHLAALGGSLQSIAIAKSGIIKQGRPVVIGGPFAADIEQIIRDRAFLTQSPVI 281

Query: 658  SSCAPGIKSSISRLDCTGSIPYQYADISINLDEKNPNLMIDLAGVKIGMLGNHQLQNAIT 479
            S+C PG+KS    +D     PYQ   I+I +    P L I++  + + +LG+HQ QNA+T
Sbjct: 282  SACDPGVKSFTKCIDWDNGKPYQRCYINIKISNDVP-LSIEMQDINLQLLGDHQRQNAVT 340

Query: 478  AICTSLCLREQGLRISDKSIITGLEKTSLLGRFQVLTPAEAESLGVVG-STIILDGAHTT 302
            A C +LCLR +G  ISD SI  GLE+T L GR Q LT  EA  LG+ G ST+++DGAHT 
Sbjct: 341  AACAALCLRSEGWDISDASIQAGLEETQLPGRSQFLTAEEASVLGLNGASTVLIDGAHTE 400

Query: 301  ASAKALADTIRRVYPDKPLAYVVSMANDKDHSGFARELISGTTPKVALFRDVSVAGSRSR 122
             SAKAL+  I+ V P+ PLA VV MA DK+H  FA +L+SG TP V L  + S+AG  SR
Sbjct: 401  ESAKALSGVIKTVRPEGPLALVVGMATDKEHLAFAEQLLSGQTPDVVLLTEASIAGGTSR 460

Query: 121  TMPASLLTDTWTQTASEMMFSVTNCSNMTRKEPNKH 14
             MPAS L + W   A +      +   ++  E  +H
Sbjct: 461  AMPASSLKEVWIAAARDHGIECVDIGGVSGAEAPEH 496


>gb|AFW56474.1| GLA1 [Zea mays]
          Length = 633

 Score =  470 bits (1210), Expect = e-130
 Identities = 247/454 (54%), Positives = 316/454 (69%), Gaps = 1/454 (0%)
 Frame = -1

Query: 1366 LQDLYNFMESLKNYEKEGIPKGAGTDSEEGFDLQRMERLIQLLGDPLSKYPVVHIAGTKG 1187
            L   +++ME L+NYE+ G+P+GAGTDS++GFDL RM RL++ LGDP + +P VHIAGTKG
Sbjct: 114  LAGFFDYMERLRNYERSGVPRGAGTDSDDGFDLGRMRRLLRRLGDPHTHFPAVHIAGTKG 173

Query: 1186 KGSTSAFISNILREEGYSVGVYTSPHLKTIRERIVTGKKCEIMSADALKNLFDDVKHMLK 1007
            KGST+AF+SNI+R +GY+VG Y+SPHL TIRERI  G     +    L +LFD+ K  + 
Sbjct: 174  KGSTAAFLSNIMRAQGYNVGCYSSPHLLTIRERISVGNDGGPVPVRLLSDLFDEAKKAID 233

Query: 1006 DALEVEGLCLTHFEVFTALAFRFFADQNVDIAVIEAGLGGARDATNIIKSEGLLASVITT 827
            +A+E E   LTHFEVFTAL+F  F+ QNVDIA+IEAGLGGARDATN+I+S  L ASVITT
Sbjct: 234  EAIESENGSLTHFEVFTALSFLLFSQQNVDIAIIEAGLGGARDATNVIRSTELAASVITT 293

Query: 826  IGEEHLEALGGSLENIAVAKSGIIKQGRPVVLGSRFEFHIEQILRNRAALLNAPIISSCA 647
            +G EHL ALGGSL++IA+AKSGIIKQGRPVV+G  F   IEQI+R+RA L  +P+IS+C 
Sbjct: 294  VGREHLVALGGSLQSIAIAKSGIIKQGRPVVIGGPFSEDIEQIIRDRAFLTQSPVISACG 353

Query: 646  PGIKSSISRLDCTGSIPYQYADISINLDEKNPNLMIDLAGVKIGMLGNHQLQNAITAICT 467
             GIKS    +D     PYQ   ISI +    P L I++  + + +LG+HQ QNA+TA C 
Sbjct: 354  HGIKSFTKCIDWDNGKPYQRCYISIKISRDVP-LSIEMHDINLQLLGDHQRQNAVTAACA 412

Query: 466  SLCLREQGLRISDKSIITGLEKTSLLGRFQVLTPAEAESLGV-VGSTIILDGAHTTASAK 290
            +LCL  QG  ISD SI  GLE+T L GR Q LT  EA +LG    ST+++DGAHT  SAK
Sbjct: 413  ALCLCSQGWDISDASIQAGLEETQLPGRSQFLTAEEASALGFDSASTVLIDGAHTEESAK 472

Query: 289  ALADTIRRVYPDKPLAYVVSMANDKDHSGFARELISGTTPKVALFRDVSVAGSRSRTMPA 110
            AL+  I+ V P+ PLA VV MA+DK+H  FA +L+SG TP V L  + SVAG  SR MPA
Sbjct: 473  ALSGVIKTVRPEGPLALVVGMASDKEHLAFAEQLLSGQTPDVVLLTEASVAGGTSRAMPA 532

Query: 109  SLLTDTWTQTASEMMFSVTNCSNMTRKEPNKHSS 8
            S L + W   A +      +   ++  E  +H S
Sbjct: 533  SSLKEVWIAAARDRGIEYVDIGGVSGAETPEHIS 566


>ref|NP_001151629.1| LOC100285263 [Zea mays] gi|195648214|gb|ACG43575.1| GLA1 [Zea mays]
          Length = 559

 Score =  470 bits (1210), Expect = e-130
 Identities = 247/454 (54%), Positives = 316/454 (69%), Gaps = 1/454 (0%)
 Frame = -1

Query: 1366 LQDLYNFMESLKNYEKEGIPKGAGTDSEEGFDLQRMERLIQLLGDPLSKYPVVHIAGTKG 1187
            L   +++ME L+NYE+ G+P+GAGTDS++GFDL RM RL++ LGDP + +P VHIAGTKG
Sbjct: 40   LAGFFDYMERLRNYERSGVPRGAGTDSDDGFDLGRMRRLLRRLGDPHTHFPAVHIAGTKG 99

Query: 1186 KGSTSAFISNILREEGYSVGVYTSPHLKTIRERIVTGKKCEIMSADALKNLFDDVKHMLK 1007
            KGST+AF+SNI+R +GY+VG Y+SPHL TIRERI  G     +    L +LFD+ K  + 
Sbjct: 100  KGSTAAFLSNIMRAQGYNVGCYSSPHLLTIRERISVGNDGGPVPVRLLSDLFDEAKKAID 159

Query: 1006 DALEVEGLCLTHFEVFTALAFRFFADQNVDIAVIEAGLGGARDATNIIKSEGLLASVITT 827
            +A+E E   LTHFEVFTAL+F  F+ QNVDIA+IEAGLGGARDATN+I+S  L ASVITT
Sbjct: 160  EAIESENGSLTHFEVFTALSFLLFSQQNVDIAIIEAGLGGARDATNVIRSTELAASVITT 219

Query: 826  IGEEHLEALGGSLENIAVAKSGIIKQGRPVVLGSRFEFHIEQILRNRAALLNAPIISSCA 647
            +G EHL ALGGSL++IA+AKSGIIKQGRPVV+G  F   IEQI+R+RA L  +P+IS+C 
Sbjct: 220  VGREHLVALGGSLQSIAIAKSGIIKQGRPVVIGGPFSEDIEQIIRDRAFLTQSPVISACG 279

Query: 646  PGIKSSISRLDCTGSIPYQYADISINLDEKNPNLMIDLAGVKIGMLGNHQLQNAITAICT 467
             GIKS    +D     PYQ   ISI +    P L I++  + + +LG+HQ QNA+TA C 
Sbjct: 280  HGIKSFTKCIDWDNGKPYQRCYISIKISRDVP-LSIEMHDINLQLLGDHQRQNAVTAACA 338

Query: 466  SLCLREQGLRISDKSIITGLEKTSLLGRFQVLTPAEAESLGV-VGSTIILDGAHTTASAK 290
            +LCL  QG  ISD SI  GLE+T L GR Q LT  EA +LG    ST+++DGAHT  SAK
Sbjct: 339  ALCLCSQGWDISDASIQAGLEETQLPGRSQFLTAEEASALGFDSASTVLIDGAHTEESAK 398

Query: 289  ALADTIRRVYPDKPLAYVVSMANDKDHSGFARELISGTTPKVALFRDVSVAGSRSRTMPA 110
            AL+  I+ V P+ PLA VV MA+DK+H  FA +L+SG TP V L  + SVAG  SR MPA
Sbjct: 399  ALSGVIKTVRPEGPLALVVGMASDKEHLAFAEQLLSGQTPDVVLLTEASVAGGTSRAMPA 458

Query: 109  SLLTDTWTQTASEMMFSVTNCSNMTRKEPNKHSS 8
            S L + W   A +      +   ++  E  +H S
Sbjct: 459  SSLKEVWIAAARDRGIEYVDIGGVSGAETPEHIS 492


>ref|XP_006374333.1| hypothetical protein POPTR_0015s06150g [Populus trichocarpa]
            gi|550322092|gb|ERP52130.1| hypothetical protein
            POPTR_0015s06150g [Populus trichocarpa]
          Length = 515

 Score =  470 bits (1209), Expect = e-130
 Identities = 245/437 (56%), Positives = 316/437 (72%)
 Frame = -1

Query: 1378 EDEKLQDLYNFMESLKNYEKEGIPKGAGTDSEEGFDLQRMERLIQLLGDPLSKYPVVHIA 1199
            E+ + ++  ++++SLKNYEK G+PK AGTDS++G DL RM RL+  LG+P SK+  VH+A
Sbjct: 11   EEPEPKEFIDYLDSLKNYEKLGVPKDAGTDSDDGLDLGRMRRLMDRLGNPQSKFKAVHVA 70

Query: 1198 GTKGKGSTSAFISNILREEGYSVGVYTSPHLKTIRERIVTGKKCEIMSADALKNLFDDVK 1019
            GTKGKGST+A++SNILR EGYSVG YTSPH+ +IRERI  G+    +S   L  LF  +K
Sbjct: 71   GTKGKGSTAAYLSNILRAEGYSVGCYTSPHMMSIRERISLGQSGNPVSTKTLNKLFHMIK 130

Query: 1018 HMLKDALEVEGLCLTHFEVFTALAFRFFADQNVDIAVIEAGLGGARDATNIIKSEGLLAS 839
              L +A+++E   LTHFEV TA AF   A++ VDIAVIEAGLGGARDATNI+ S  L AS
Sbjct: 131  PKLDEAIQLENGSLTHFEVLTATAFTLMAEEKVDIAVIEAGLGGARDATNILCSSELAAS 190

Query: 838  VITTIGEEHLEALGGSLENIAVAKSGIIKQGRPVVLGSRFEFHIEQILRNRAALLNAPII 659
            VITTIGEEHL ALGGSLE+IAVAKSGIIK GRPVVLG  F  H+++ILR++A+++ +P++
Sbjct: 191  VITTIGEEHLAALGGSLESIAVAKSGIIKYGRPVVLGGPFLSHVDRILRDKASVMCSPVV 250

Query: 658  SSCAPGIKSSISRLDCTGSIPYQYADISINLDEKNPNLMIDLAGVKIGMLGNHQLQNAIT 479
            S+   GI++SI  L      P Q +DI I ++   P L I+L+ VK+ MLG HQL NA +
Sbjct: 251  SASDAGIRTSIKGLIILDGRPCQLSDIMIQVERDFP-LFIELSDVKLRMLGRHQLHNASS 309

Query: 478  AICTSLCLREQGLRISDKSIITGLEKTSLLGRFQVLTPAEAESLGVVGSTIILDGAHTTA 299
            A C +LCLR+QG RISD+SI  GLE T LLGR Q L+  E E LG+ G+TI+LDGAHT  
Sbjct: 310  AACVALCLRDQGCRISDRSIRAGLENTFLLGRSQFLSSKETEVLGLPGATILLDGAHTKD 369

Query: 298  SAKALADTIRRVYPDKPLAYVVSMANDKDHSGFARELISGTTPKVALFRDVSVAGSRSRT 119
            SAKAL DT+R  +PD  +A VV+MA+DKDH  FARE +SG   +     +  +AG +SRT
Sbjct: 370  SAKALVDTVRMAFPDARVALVVAMASDKDHLAFAREFLSGLQLEAVFLTEADIAGGKSRT 429

Query: 118  MPASLLTDTWTQTASEM 68
              ASLL D W Q + E+
Sbjct: 430  TSASLLMDCWIQASEEL 446


>ref|XP_002331843.1| folylpolyglutamate synthase [Populus trichocarpa]
          Length = 478

 Score =  470 bits (1209), Expect = e-130
 Identities = 245/437 (56%), Positives = 316/437 (72%)
 Frame = -1

Query: 1378 EDEKLQDLYNFMESLKNYEKEGIPKGAGTDSEEGFDLQRMERLIQLLGDPLSKYPVVHIA 1199
            E+ + ++  ++++SLKNYEK G+PK AGTDS++G DL RM RL+  LG+P SK+  VH+A
Sbjct: 11   EEPEPKEFIDYLDSLKNYEKLGVPKDAGTDSDDGLDLGRMRRLMDRLGNPQSKFKAVHVA 70

Query: 1198 GTKGKGSTSAFISNILREEGYSVGVYTSPHLKTIRERIVTGKKCEIMSADALKNLFDDVK 1019
            GTKGKGST+A++SNILR EGYSVG YTSPH+ +IRERI  G+    +S   L  LF  +K
Sbjct: 71   GTKGKGSTAAYLSNILRAEGYSVGCYTSPHMMSIRERISLGQSGNPVSTKTLNKLFHMIK 130

Query: 1018 HMLKDALEVEGLCLTHFEVFTALAFRFFADQNVDIAVIEAGLGGARDATNIIKSEGLLAS 839
              L +A+++E   LTHFEV TA AF   A++ VDIAVIEAGLGGARDATNI+ S  L AS
Sbjct: 131  PKLDEAIQLENGSLTHFEVLTATAFTLMAEEKVDIAVIEAGLGGARDATNILCSSELAAS 190

Query: 838  VITTIGEEHLEALGGSLENIAVAKSGIIKQGRPVVLGSRFEFHIEQILRNRAALLNAPII 659
            VITTIGEEHL ALGGSLE+IAVAKSGIIK GRPVVLG  F  H+++ILR++A+++ +P++
Sbjct: 191  VITTIGEEHLAALGGSLESIAVAKSGIIKYGRPVVLGGPFLSHVDRILRDKASVMCSPVV 250

Query: 658  SSCAPGIKSSISRLDCTGSIPYQYADISINLDEKNPNLMIDLAGVKIGMLGNHQLQNAIT 479
            S+   GI++SI  L      P Q +DI I ++   P L I+L+ VK+ MLG HQL NA +
Sbjct: 251  SASDAGIRTSIKGLIILDGRPCQLSDIMIQVERDFP-LFIELSDVKLRMLGRHQLHNASS 309

Query: 478  AICTSLCLREQGLRISDKSIITGLEKTSLLGRFQVLTPAEAESLGVVGSTIILDGAHTTA 299
            A C +LCLR+QG RISD+SI  GLE T LLGR Q L+  E E LG+ G+TI+LDGAHT  
Sbjct: 310  AACVALCLRDQGCRISDRSIRAGLENTFLLGRSQFLSSKETEVLGLPGATILLDGAHTKD 369

Query: 298  SAKALADTIRRVYPDKPLAYVVSMANDKDHSGFARELISGTTPKVALFRDVSVAGSRSRT 119
            SAKAL DT+R  +PD  +A VV+MA+DKDH  FARE +SG   +     +  +AG +SRT
Sbjct: 370  SAKALVDTVRMAFPDARVALVVAMASDKDHLAFAREFLSGLQLEAVFLTEADIAGGKSRT 429

Query: 118  MPASLLTDTWTQTASEM 68
              ASLL D W Q + E+
Sbjct: 430  TSASLLMDCWIQASEEL 446


>tpg|DAA55274.1| TPA: hypothetical protein ZEAMMB73_929164 [Zea mays]
          Length = 557

 Score =  468 bits (1203), Expect = e-129
 Identities = 244/452 (53%), Positives = 314/452 (69%), Gaps = 1/452 (0%)
 Frame = -1

Query: 1366 LQDLYNFMESLKNYEKEGIPKGAGTDSEEGFDLQRMERLIQLLGDPLSKYPVVHIAGTKG 1187
            L   +++ME L+NYE+ G+P+GAGTDS++GFDL RM RL++ LGDP + +P VHIAGTKG
Sbjct: 39   LGGFFDYMERLRNYERSGVPRGAGTDSDDGFDLGRMRRLLRRLGDPHTDFPAVHIAGTKG 98

Query: 1186 KGSTSAFISNILREEGYSVGVYTSPHLKTIRERIVTGKKCEIMSADALKNLFDDVKHMLK 1007
            KGST+A +SNI+R +GY+VG Y+SPHL TIRERI  G     +    L +LFD  K  + 
Sbjct: 99   KGSTAALLSNIMRAQGYNVGSYSSPHLLTIRERISVGNDGGPVPVRLLSDLFDGAKEAID 158

Query: 1006 DALEVEGLCLTHFEVFTALAFRFFADQNVDIAVIEAGLGGARDATNIIKSEGLLASVITT 827
            +A+E+E   LTHFEVFTAL+F  F+ +NVDIA+IEAGLGGARDATN+I+S  L ASVITT
Sbjct: 159  EAIELENGSLTHFEVFTALSFLLFSQENVDIAIIEAGLGGARDATNVIRSTELAASVITT 218

Query: 826  IGEEHLEALGGSLENIAVAKSGIIKQGRPVVLGSRFEFHIEQILRNRAALLNAPIISSCA 647
            +G EHL ALGGSL++IA+AKSGIIKQG+PVV+G  F   IEQI+R+RA L  +P+IS+C 
Sbjct: 219  VGREHLVALGGSLQSIAIAKSGIIKQGQPVVIGGPFSAVIEQIIRDRAFLTQSPVISACD 278

Query: 646  PGIKSSISRLDCTGSIPYQYADISINLDEKNPNLMIDLAGVKIGMLGNHQLQNAITAICT 467
            PGIKS    +D     PYQ    SIN+    P L I++  + + +LG+HQ QNA+TA CT
Sbjct: 279  PGIKSFTKCIDWDNGKPYQRCYFSINISNDVP-LSIEMHDINLQLLGDHQRQNAVTAACT 337

Query: 466  SLCLREQGLRISDKSIITGLEKTSLLGRFQVLTPAEAESLGVVG-STIILDGAHTTASAK 290
            +LCLR  G  ISD SI  GL +T L GR Q LT  EA  LG+ G ST+++DGAHT  SAK
Sbjct: 338  ALCLRSLGWEISDASIQAGLRETQLPGRSQFLTAEEASVLGLDGASTVLIDGAHTEESAK 397

Query: 289  ALADTIRRVYPDKPLAYVVSMANDKDHSGFARELISGTTPKVALFRDVSVAGSRSRTMPA 110
            AL+  I  V P+ PLA VV MA+DK+H  FA +L+SG TP V L  + S+AG  SR MPA
Sbjct: 398  ALSGVIEAVRPEGPLALVVGMASDKEHLAFAEQLLSGQTPDVVLLTEASIAGGSSRAMPA 457

Query: 109  SLLTDTWTQTASEMMFSVTNCSNMTRKEPNKH 14
            S L + W   A        +   ++  E  +H
Sbjct: 458  SSLKEVWIAAARHRGIEYVDIGGISGTETLEH 489


>gb|EOY23454.1| Folylpolyglutamate synthetase family protein isoform 2 [Theobroma
            cacao]
          Length = 549

 Score =  466 bits (1200), Expect = e-129
 Identities = 243/441 (55%), Positives = 318/441 (72%)
 Frame = -1

Query: 1378 EDEKLQDLYNFMESLKNYEKEGIPKGAGTDSEEGFDLQRMERLIQLLGDPLSKYPVVHIA 1199
            E+ +L+D   +++SLKNYEK G+PK AGTDS++GFDL RM RL+  LG+P S +  VHIA
Sbjct: 35   EEPELKDFIQYIDSLKNYEKSGVPKDAGTDSDDGFDLGRMRRLMDRLGNPQSNFKSVHIA 94

Query: 1198 GTKGKGSTSAFISNILREEGYSVGVYTSPHLKTIRERIVTGKKCEIMSADALKNLFDDVK 1019
            GTKGKGST+A++SNILR EGYSVG YTSPH+ +IRER+  G+  + +S++ L  LF  +K
Sbjct: 95   GTKGKGSTAAYLSNILRTEGYSVGCYTSPHILSIRERMSVGRLGKPVSSNTLNCLFHRIK 154

Query: 1018 HMLKDALEVEGLCLTHFEVFTALAFRFFADQNVDIAVIEAGLGGARDATNIIKSEGLLAS 839
              L +A+ +E  CL+HFEV TA+AF  FA +NVDIA+IEAGLGGARDATNII S  L AS
Sbjct: 155  QSLDEAIILENGCLSHFEVLTAVAFTLFAQENVDIAIIEAGLGGARDATNIISSSELAAS 214

Query: 838  VITTIGEEHLEALGGSLENIAVAKSGIIKQGRPVVLGSRFEFHIEQILRNRAALLNAPII 659
            +ITTIGEEHL ALGGSLE+IA+AK+GIIK GRP++LG  F  HI+ ILR++A  +++PI+
Sbjct: 215  IITTIGEEHLAALGGSLESIAMAKAGIIKHGRPLILGGPFLPHIDCILRDKALSMSSPIV 274

Query: 658  SSCAPGIKSSISRLDCTGSIPYQYADISINLDEKNPNLMIDLAGVKIGMLGNHQLQNAIT 479
            S+   GI+++I  +      P Q  D+ I LD  +  L I+L  + + MLG HQLQNA+T
Sbjct: 275  SASDSGIRTAIKGVSTFKGRPSQSCDLMIQLD-CDFQLSIELCDLNLSMLGTHQLQNAVT 333

Query: 478  AICTSLCLREQGLRISDKSIITGLEKTSLLGRFQVLTPAEAESLGVVGSTIILDGAHTTA 299
            A CT+LCL  QG +ISD SI  GLE T L GR Q LT  EAE+LG+ G+T+++DGAHT  
Sbjct: 334  AACTALCLCNQGWKISDGSIRAGLENTCLQGRSQFLTSKEAETLGLPGATVLIDGAHTKE 393

Query: 298  SAKALADTIRRVYPDKPLAYVVSMANDKDHSGFARELISGTTPKVALFRDVSVAGSRSRT 119
            SAKAL DTI+  +PD  LA VV+MA DKDH  FA+EL+SG   +     + ++AG  SRT
Sbjct: 394  SAKALLDTIQMTFPDSRLAIVVAMACDKDHLAFAKELLSGRQVEAVFLTESNIAGGTSRT 453

Query: 118  MPASLLTDTWTQTASEMMFSV 56
              AS+L D W Q + E+   V
Sbjct: 454  TSASVLRDCWMQASRELGIKV 474