BLASTX nr result
ID: Ephedra25_contig00019627
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Ephedra25_contig00019627
(915 letters)
Database: ./nr
37,332,560 sequences; 13,225,080,153 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|H8ZM73.1|CAS_ABIBA RecName: Full=Bifunctional cis-abienol syn... 122 2e-25
sp|Q675L0.1|TPSLS_PICAB RecName: Full=Longifolene synthase; Shor... 120 5e-25
sp|O64404.1|TPSD4_ABIGR RecName: Full=Delta-selinene synthase; A... 120 7e-25
gb|ACN39765.1| unknown [Picea sitchensis] gi|325930026|gb|ADZ455... 120 7e-25
gb|ACN40827.1| unknown [Picea sitchensis] 120 9e-25
gb|AAK39129.2|AF369920_1 gamma-humulene synthase-like protein [P... 120 9e-25
gb|ADZ45515.1| longifolene synthase [Picea engelmannii x Picea g... 119 2e-24
gb|AAK83561.1| delta-selinene synthase [Abies grandis] 118 3e-24
gb|ABV44453.1| 1(10),5-germacradien-4-ol synthase [Pinus sylvest... 117 5e-24
gb|ADZ45513.1| alpha-humulene synthase [Picea glauca] 115 2e-23
sp|H8ZM70.1|LAS_ABIBA RecName: Full=Bifunctional abietadiene syn... 115 2e-23
gb|AAK83563.1| abietadiene synthase [Abies grandis] 115 3e-23
sp|Q38710.1|TPSDV_ABIGR RecName: Full=Abietadiene synthase, chlo... 114 4e-23
pdb|3S9V|A Chain A, Abietadiene Synthase From Abies Grandis gi|3... 114 4e-23
gb|ADZ45517.1| levopimaradiene/abietadiene synthase [Picea sitch... 113 9e-23
gb|ACN40849.1| unknown [Picea sitchensis] 113 9e-23
gb|ABV44452.1| caryophyllene/humulene synthase [Pinus sylvestris] 112 2e-22
sp|Q84LB2.2|AFS1_MALDO RecName: Full=(E,E)-alpha-farnesene synth... 112 2e-22
gb|AFU73863.1| levopimaradiene/abietadiene synthase [Pinus conto... 112 3e-22
gb|AFU73862.1| levopimaradiene/abietadiene synthase [Pinus conto... 112 3e-22
>sp|H8ZM73.1|CAS_ABIBA RecName: Full=Bifunctional cis-abienol synthase, chloroplastic;
AltName: Full=Diterpene synthase TPS4; Short=AbdiTPS4;
Flags: Precursor gi|343174804|gb|AEL99953.1| diterpene
synthase TPS4 [Abies balsamea]
Length = 867
Score = 122 bits (305), Expect = 2e-25
Identities = 75/264 (28%), Positives = 133/264 (50%), Gaps = 1/264 (0%)
Frame = -3
Query: 907 SAVSKLKIFRQNLLESYMMVVLGNFEPELSDFRIILTKAIMTVFILDNIYEYQLNVNQVK 728
S ++LK R ++E+Y V FEPE S R + TK + + ILD++Y+ + +++K
Sbjct: 576 SGFTELKFTRDRVVETYFAVASSMFEPEFSTCRAVYTKISVLLVILDDLYDGYGSPDEIK 635
Query: 727 EFTKAVRRQKLSMKETFSEEVKICFIFVMETYKEIINSCSIKQGYNMEQILLPKWIRQLE 548
F++AV+R LS+ E + +KICF+ + T E+ QG+++ + W QL
Sbjct: 636 LFSEAVKRWDLSLLEQMPDHMKICFLGLYNTVNEVAEEGRKTQGHDVLGYIRNLWEIQLA 695
Query: 547 AFELEATWFTEREIPSMEVYLRNGCVSVSVWPIFTIARLLTCKFSCHYKDLKNELKINTE 368
AF EA W + +PS + Y+ N VS+ V I I L T D+ + + ++
Sbjct: 696 AFTREAEWSQGKYVPSFDEYIENAQVSIGVATILLITILFT-----EEDDILSHIDYGSK 750
Query: 367 LYKLCAQSVRIHNDLLEELKEALVTKEISVIDCYMKERPLEKREDVTKALRSLVREHGKT 188
+L + + R+ ND+ +E + +S I CYMK+RP E+ K + +
Sbjct: 751 FLRLASLTARLANDIKTYQEERAHGEVVSAIQCYMKDRPEITEEEALKYVYGRMVNDLAE 810
Query: 187 LSKLFMDPNGI-EEYERVAFELTR 119
L+ ++ N + + +R+ F+ R
Sbjct: 811 LNSEYLKSNEMPQNCKRLVFDTAR 834
>sp|Q675L0.1|TPSLS_PICAB RecName: Full=Longifolene synthase; Short=PaTPS-Lon
gi|44804575|gb|AAS47695.1| longifolene synthase [Picea
abies]
Length = 578
Score = 120 bits (302), Expect = 5e-25
Identities = 76/278 (27%), Positives = 144/278 (51%), Gaps = 6/278 (2%)
Frame = -3
Query: 913 NQSAVSKLKIFRQNLLESYMMVVLGNFEPELSDFRIILTKAIMTVFILDNIYEYQLNVNQ 734
+QS +++ +R+ +E Y+ VV+G FEPE S RI K + ILD++Y+ + Q
Sbjct: 283 SQSDIAQQNFYRKRHVEFYLWVVIGTFEPEFSTCRITFAKISTLMTILDDLYDTHGTLEQ 342
Query: 733 VKEFTKAVRRQKLSMKETFSEEVKICFIFVMETYKEIINSCSIKQGYNMEQILLPKWIRQ 554
+K FT+ V+R LS+ + + +KI F F + T E+I + Q +M + W R
Sbjct: 343 LKIFTEGVKRWDLSLVDRLPDYIKITFEFFLNTSNELIAEVAKTQERDMSAYIRKTWERY 402
Query: 553 LEAFELEATWFTEREIPSMEVYLRNGCVS-----VSVWPIFTIARLLTCKFSCHYKDLKN 389
LEA+ EA W R +P+ + Y++NG S ++++ + + +LL D+
Sbjct: 403 LEAYLQEAEWIAARHVPTFDEYMKNGISSSGMCILNLYSLLLMGQLLP-------DDVLE 455
Query: 388 ELKINTELYKLCAQSVRIHNDLLEELKEALVTKEISVIDCYMKERPLEKREDVTKALRSL 209
++ +++++L + R+ +D + + + + S I+CY+K+ P ED + L L
Sbjct: 456 QIHSPSKIHELVELTARLVDDSKDFETKKVGGELASGIECYVKDNPECTLEDASNHLNGL 515
Query: 208 VREHGKTLSKLFMDPNGIEE-YERVAFELTRALHFLYK 98
+ K L+ F+ + + +++ AF + R L +YK
Sbjct: 516 LDLTVKELNWEFVRHDSVALCFKKFAFNVARGLRLIYK 553
>sp|O64404.1|TPSD4_ABIGR RecName: Full=Delta-selinene synthase; AltName: Full=Agfdsel1
gi|2961460|gb|AAC05727.1| d-selinene synthase [Abies
grandis]
Length = 581
Score = 120 bits (301), Expect = 7e-25
Identities = 81/304 (26%), Positives = 150/304 (49%), Gaps = 7/304 (2%)
Frame = -3
Query: 907 SAVSKLKIFRQNLLESYMMVVLGNFEPELSDFRIILTKAIMTVFILDNIYEYQLNVNQVK 728
S +S+L +R+ +E Y VV+ FEPE S+ RI K + +LD++Y+ ++++K
Sbjct: 285 SGISQLNFYRKRHVEYYSWVVMCIFEPEFSESRIAFAKTAILCTVLDDLYDTHATLHEIK 344
Query: 727 EFTKAVRRQKLSMKETFSEEVKICFIFVMETYKEIINSCSIKQGYNMEQILLPKWIRQLE 548
T+ VRR LS+ + + +KI F F T E+I +QG +M I+ W R +E
Sbjct: 345 IMTEGVRRWDLSLTDDLPDYIKIAFQFFFNTVNELIVEIVKRQGRDMTTIVKDCWKRYIE 404
Query: 547 AFELEATWFTEREIPSMEVYLRNGCVS-----VSVWPIFTIARLLTCKFSCHYKDLKNEL 383
++ EA W IP+ Y++NG S +++ P+ + +LL ++ ++
Sbjct: 405 SYLQEAEWIATGHIPTFNEYIKNGMASSGMCILNLNPLLLLDKLLP-------DNILEQI 457
Query: 382 KINTELYKLCAQSVRIHNDLLEELKEALVTKEISVIDCYMKERPLEKREDVTKALRSLVR 203
+++ L + RI +DL + E + S + CYMKE P E+ ++ ++
Sbjct: 458 HSPSKILDLLELTGRIADDLKDFEDEKERGEMASSLQCYMKENPESTVENALNHIKGILN 517
Query: 202 EHGKTLSKLFMDPNGIEE-YERVAFELTRALHFLYK-RA*CFTSYEGDKELNFKECAHRL 29
+ + FM + + ++ F + R L F+YK R + S + K+ FK H++
Sbjct: 518 RSLEEFNWEFMKQDSVPMCCKKFTFNIGRGLQFIYKYRDGLYISDKEVKDQIFKILVHQV 577
Query: 28 FLQE 17
++E
Sbjct: 578 PMEE 581
>gb|ACN39765.1| unknown [Picea sitchensis] gi|325930026|gb|ADZ45516.1|
alpha-longipinene synthase [Picea sitchensis]
Length = 579
Score = 120 bits (301), Expect = 7e-25
Identities = 76/278 (27%), Positives = 143/278 (51%), Gaps = 6/278 (2%)
Frame = -3
Query: 913 NQSAVSKLKIFRQNLLESYMMVVLGNFEPELSDFRIILTKAIMTVFILDNIYEYQLNVNQ 734
+QS +++ +R+ +E Y VV+G FEPE S RI K + ILD++Y+ + Q
Sbjct: 284 SQSDIAQQNFYRKRHVEFYFWVVIGTFEPEFSTCRITFAKIATLMTILDDLYDTHGTLEQ 343
Query: 733 VKEFTKAVRRQKLSMKETFSEEVKICFIFVMETYKEIINSCSIKQGYNMEQILLPKWIRQ 554
+K FT+ V+R LS+ ++ + +KI F F + T E+I + Q +M + W R
Sbjct: 344 LKIFTEGVKRWDLSLVDSLPDYIKITFEFFLNTSNELIAEVAKTQERDMSAYIRKTWERY 403
Query: 553 LEAFELEATWFTEREIPSMEVYLRNGCVS-----VSVWPIFTIARLLTCKFSCHYKDLKN 389
LEA+ EA W T +P+ + Y++NG S ++++ + + +LL D+
Sbjct: 404 LEAYMQEAEWITAGHVPTFDEYMKNGISSSGMCILNLYSLLLMGQLLP-------DDVLE 456
Query: 388 ELKINTELYKLCAQSVRIHNDLLEELKEALVTKEISVIDCYMKERPLEKREDVTKALRSL 209
++ +++++L + R+ +D + + + S I+CY+K+ P ED + L L
Sbjct: 457 QIHSPSKIHELVELTARLVDDSKDFETNKVGGELASGIECYVKDNPECTLEDASNHLNGL 516
Query: 208 VREHGKTLSKLFMDPNGIEE-YERVAFELTRALHFLYK 98
+ K L+ F+ + + +++ AF + R L +YK
Sbjct: 517 LDLTVKELNWEFVRHDSVALCFKKFAFNVARGLRLIYK 554
>gb|ACN40827.1| unknown [Picea sitchensis]
Length = 654
Score = 120 bits (300), Expect = 9e-25
Identities = 75/277 (27%), Positives = 140/277 (50%), Gaps = 7/277 (2%)
Frame = -3
Query: 907 SAVSKLKIFRQNLLESYMMVVLGNFEPELSDFRIILTKAIMTVFILDNIYEYQLNVNQVK 728
S + +L +R+ +E Y VV G FEPE S RI TK +LD++Y+ +++VK
Sbjct: 359 SYLPQLGFYRKRHVELYFWVVTGTFEPEFSSSRIAFTKIATLATVLDDLYDTHGTLDEVK 418
Query: 727 EFTKAVRRQKLSMKETFSEEVKICFIFVMETYKEIINSCSIKQGYNMEQILLPK-WIRQL 551
FT+ VRR S+ + +K F F M+T E+ + KQG +M + W R L
Sbjct: 419 IFTEGVRRWDNSLIGRLPDHIKKIFEFFMKTSNEMNDEVEKKQGRDMRAYIRKNGWERYL 478
Query: 550 EAFELEATWFTEREIPSMEVYLRNGCVS-----VSVWPIFTIARLLTCKFSCHYKDLKNE 386
E++ EA W +PS YL+NG S +++ P+ + ++L +D+ +
Sbjct: 479 ESYLQEAEWIAAGYVPSFNEYLKNGLASSGSCVLNLIPLLLMGQILP-------EDILEQ 531
Query: 385 LKINTELYKLCAQSVRIHNDLLEELKEALVTKEISVIDCYMKERPLEKREDVTKALRSLV 206
+ +++++L ++R+ +D+ + +E + S ++CY+K+ P RED ++ ++
Sbjct: 532 IFFPSKIHELLEMTIRLKDDITDFEQEKERGEIASSLECYLKDNPESTREDALNHIKGIL 591
Query: 205 REHGKTLSKLFMDPNGIEE-YERVAFELTRALHFLYK 98
L+ F+ + + ++ F R +HFL+K
Sbjct: 592 DLSVSELNWEFLKHDNVPLCCKKFTFNFARGMHFLFK 628
>gb|AAK39129.2|AF369920_1 gamma-humulene synthase-like protein [Picea abies]
Length = 577
Score = 120 bits (300), Expect = 9e-25
Identities = 76/278 (27%), Positives = 145/278 (52%), Gaps = 6/278 (2%)
Frame = -3
Query: 913 NQSAVSKLKIFRQNLLESYMMVVLGNFEPELSDFRIILTKAIMTVFILDNIYEYQLNVNQ 734
+QS +++ +R+ +E Y+ VV+G FEPE S RI K + ILD++Y+ + Q
Sbjct: 283 SQSDIAQQNFYRKRHVEFYLWVVIGTFEPEFSTCRITFAKIATLMTILDDLYDTHGTLEQ 342
Query: 733 VKEFTKAVRRQKLSMKETFSEEVKICFIFVMETYKEIINSCSIKQGYNMEQILLPKWIRQ 554
+K FT+ V+R LS+ + + +KI F F + T E+I + Q +M + W R
Sbjct: 343 LKIFTEGVKRWDLSLVDRLPDYIKITFEFFLNTSNELIAEVAKTQERDMSAYIRKTWERY 402
Query: 553 LEAFELEATWFTEREIPSMEVYLRNGCVS-----VSVWPIFTIARLLTCKFSCHYKDLKN 389
LEA+ EA W R +P+ + Y++NG S ++++ + + +LL D+
Sbjct: 403 LEAYLQEAEWIAARHVPTFDEYMKNGISSSGMCILNLYSLLLMGQLLP-------DDVLE 455
Query: 388 ELKINTELYKLCAQSVRIHNDLLEELKEALVTKEISVIDCYMKERPLEKREDVTKALRSL 209
++ +++++L + R+ +D ++ + + + S I+CY+K+ P ED + L L
Sbjct: 456 QIHSPSKIHELVELTARLVDD-SKDFETKVGGELASGIECYVKDNPECTLEDASNHLNGL 514
Query: 208 VREHGKTLSKLFMDPNGIEE-YERVAFELTRALHFLYK 98
+ K L+ F+ + + +++ AF + R L +YK
Sbjct: 515 LDLTVKELNWEFVRHDSVALCFKKFAFNVARGLRLIYK 552
>gb|ADZ45515.1| longifolene synthase [Picea engelmannii x Picea glauca]
Length = 578
Score = 119 bits (298), Expect = 2e-24
Identities = 76/278 (27%), Positives = 143/278 (51%), Gaps = 6/278 (2%)
Frame = -3
Query: 913 NQSAVSKLKIFRQNLLESYMMVVLGNFEPELSDFRIILTKAIMTVFILDNIYEYQLNVNQ 734
+QS +++ +R+ +E Y+ VV+G FEPE S RI K + ILD++Y+ + Q
Sbjct: 283 SQSDIAQQNFYRKRHVEYYLWVVIGTFEPEFSTCRIAFAKIATLMTILDDLYDTHGTLEQ 342
Query: 733 VKEFTKAVRRQKLSMKETFSEEVKICFIFVMETYKEIINSCSIKQGYNMEQILLPKWIRQ 554
+K FT+ V+R LS+ + + +KI F F + T E+I + Q +M + W R
Sbjct: 343 LKIFTEGVKRWDLSLVDRLPDYIKITFEFFLNTSNELIAEVAKTQERDMSAYIRKTWERY 402
Query: 553 LEAFELEATWFTEREIPSMEVYLRNGCVS-----VSVWPIFTIARLLTCKFSCHYKDLKN 389
LEA+ EA W R +P+ + Y++NG S ++++ + + +LL D+
Sbjct: 403 LEAYLQEAEWIAARHVPTFDEYMKNGISSSGMCILNLYSLLLMGQLLP-------DDVLE 455
Query: 388 ELKINTELYKLCAQSVRIHNDLLEELKEALVTKEISVIDCYMKERPLEKREDVTKALRSL 209
++ +++++L + R+ +D + + + S I+CY+K+ P ED + L L
Sbjct: 456 QIHSPSKIHELVELTARLVDDSKDFETKKAGGELASGIECYVKDNPECTLEDASNHLIGL 515
Query: 208 VREHGKTLSKLFMDPNGIEE-YERVAFELTRALHFLYK 98
+ K L+ F+ + + +++ AF + R L +YK
Sbjct: 516 LDLTVKELNWEFVRHDSVALCFKKFAFNVARGLRLIYK 553
>gb|AAK83561.1| delta-selinene synthase [Abies grandis]
Length = 579
Score = 118 bits (296), Expect = 3e-24
Identities = 82/304 (26%), Positives = 146/304 (48%), Gaps = 7/304 (2%)
Frame = -3
Query: 907 SAVSKLKIFRQNLLESYMMVVLGNFEPELSDFRIILTKAIMTVFILDNIYEYQLNVNQVK 728
S +S L +R +E Y V+G FEPE S+ RI K ILD++Y+ ++++K
Sbjct: 283 SGISDLNFYRNRHVEYYFWAVIGIFEPEFSESRIAFAKTATVCTILDDLYDTHATLDELK 342
Query: 727 EFTKAVRRQKLSMKETFSEEVKICFIFVMETYKEIINSCSIKQGYNMEQILLPKWIRQLE 548
T+ VRR LS+ + +KI F F E+I +QG +M I+ W R +E
Sbjct: 343 IMTEGVRRWDLSLIHDLPDYIKIAFQFFFNIADELIFEIVKQQGRDMTAIVKDCWKRYIE 402
Query: 547 AFELEATWFTEREIPSMEVYLRNGCVS-----VSVWPIFTIARLLTCKFSCHYKDLKNEL 383
++ EA W +P+ Y++NG S V++ P+ + +LL ++ ++
Sbjct: 403 SYLQEAEWIASGHMPTFNEYIKNGLASSGLCIVNLNPLLLLGKLLP-------DNILEQI 455
Query: 382 KINTELYKLCAQSVRIHNDLLEELKEALVTKEISVIDCYMKERPLEKREDVTKALRSLVR 203
+++ +L + RI +DL + E + S + CYMKE P E+ ++ ++
Sbjct: 456 HSPSKIQELSELTGRITDDLKDFEDEKERGEMASSLQCYMKENPESTVENALNHIKGILN 515
Query: 202 EHGKTLSKLFMDPNGIEE-YERVAFELTRALHFLYK-RA*CFTSYEGDKELNFKECAHRL 29
+ + FM + + ++ F + R L FLYK R + S + K+ FK H++
Sbjct: 516 RSLEEFNWEFMKQDSVPMCCKKFTFNIGRGLQFLYKYRDGLYISDKEVKDQIFKILVHQV 575
Query: 28 FLQE 17
++E
Sbjct: 576 PMEE 579
>gb|ABV44453.1| 1(10),5-germacradien-4-ol synthase [Pinus sylvestris]
Length = 625
Score = 117 bits (294), Expect = 5e-24
Identities = 74/274 (27%), Positives = 135/274 (49%), Gaps = 2/274 (0%)
Frame = -3
Query: 913 NQSAVSKLKIFRQNLLESYMMVVLGNFEPELSDFRIILTKAIMTVFILDNIYEYQLNVNQ 734
++S + +L +R+ +E Y VLG FEPE RI TK + ++D++Y+ +++
Sbjct: 328 SESYLPQLDFYRKRHVELYFWAVLGTFEPEFRSSRIAFTKLSTVMTVIDDLYDTHGTLDE 387
Query: 733 VKEFTKAVRRQKLSMKETFSEEVKICFIFVMETYKEIINSCSIKQGYNMEQILLPK-WIR 557
+K FT+ VRR S+ + ++ F F M+T E KQG +M + W R
Sbjct: 388 IKIFTEGVRRWDTSLISRLPDHIQKIFEFFMKTSNEWTAEVEKKQGRDMAAYIRKNGWER 447
Query: 556 QLEAFELEATWFTEREIPSMEVYLRNGCVSVSVWPIFTIARLLTCKFSCHYKDLKNELKI 377
+E++ E W +PS Y +NG S + + I LL + D+ ++
Sbjct: 448 YVESYLQEGEWMAAGYVPSFNEYYKNGLASSGMCVLNLIPLLLMDQIL--PDDILKQIVY 505
Query: 376 NTELYKLCAQSVRIHNDLLEELKEALVTKEISVIDCYMKERPLEKREDVTKALRSLVREH 197
+++++L ++R+ +D+ + KE + S +CYMK+ P RED ++ ++
Sbjct: 506 PSKIHELLELTIRVKDDITDFEKEKEHGQVASCSECYMKDNPECTREDALNHMKGILDLS 565
Query: 196 GKTLSKLFMDPNGIEE-YERVAFELTRALHFLYK 98
L+ F+ + + Y+R F L R +HFL+K
Sbjct: 566 VSQLNWEFLKHDNVPLCYKRFTFNLARGMHFLFK 599
>gb|ADZ45513.1| alpha-humulene synthase [Picea glauca]
Length = 575
Score = 115 bits (289), Expect = 2e-23
Identities = 73/276 (26%), Positives = 136/276 (49%), Gaps = 6/276 (2%)
Frame = -3
Query: 907 SAVSKLKIFRQNLLESYMMVVLGNFEPELSDFRIILTKAIMTVFILDNIYEYQLNVNQVK 728
S +++L +R+ +E Y V FEPE S RI K +LD++Y+ ++++K
Sbjct: 279 SEIAQLNFYRRRHVELYFWAVTCIFEPEFSPSRIAFAKITTVGAVLDDLYDTHGTLDELK 338
Query: 727 EFTKAVRRQKLSMKETFSEEVKICFIFVMETYKEIINSCSIKQGYNMEQILLPKWIRQLE 548
T+AVRR LS+ + +KI F T E+ KQG +M +L W R +E
Sbjct: 339 TITEAVRRWDLSLIDDLPNNIKIACQFFFNTANELAVEVVKKQGRDMTALLKATWQRYVE 398
Query: 547 AFELEATWFTEREIPSMEVYLRNGCVS-----VSVWPIFTIARLLTCKFSCHYKDLKNEL 383
++ EA W R +PS Y++N VS V++ P+ + +LL ++ ++
Sbjct: 399 SYLQEAEWIETRHVPSFNEYIKNALVSSGMCIVNLIPLLLLGQLLA-------NNIVEQI 451
Query: 382 KINTELYKLCAQSVRIHNDLLEELKEALVTKEISVIDCYMKERPLEKREDVTKALRSLVR 203
+++ +L ++R+ +D+ + E + S+++CYMK+ P E+ ++ ++
Sbjct: 452 LSPSKIQELSELTIRLIDDIRDFEDEKERGEIASIVECYMKDNPDSTLENALNHIKGILH 511
Query: 202 EHGKTLSKLFMDPNGIEE-YERVAFELTRALHFLYK 98
+ L+ FM + + ++ F + R L FLYK
Sbjct: 512 VSLEELNWEFMKDDSVPLCCKKFTFNIVRGLQFLYK 547
>sp|H8ZM70.1|LAS_ABIBA RecName: Full=Bifunctional abietadiene synthase, chloroplastic;
AltName: Full=Abietadiene cyclase; AltName:
Full=Diterpene synthase TPS1; Short=AbdiTPS1; AltName:
Full=Levopimaradiene/abietadiene synthase; Short=AbLAS;
Includes: RecName: Full=Abietadiene synthase; AltName:
Full=Levopimaradiene synthase; AltName:
Full=Neoabietadiene synthase; Includes: RecName:
Full=Copalyl diphosphate synthase; Flags: Precursor
gi|343174798|gb|AEL99950.1| diterpene synthase TPS1,
partial [Abies balsamea]
Length = 844
Score = 115 bits (288), Expect = 2e-23
Identities = 76/290 (26%), Positives = 140/290 (48%), Gaps = 1/290 (0%)
Frame = -3
Query: 907 SAVSKLKIFRQNLLESYMMVVLGNFEPELSDFRIILTKAIMTVFILDNIYEYQLNVNQVK 728
S + L R+ + E Y FEPE S R + TK ILD++Y+ +++ +K
Sbjct: 551 SGFTDLNFTRERVTEIYFSPASFIFEPEFSKCREVYTKTSNFTVILDDLYDAHGSLDDLK 610
Query: 727 EFTKAVRRQKLSMKETFSEEVKICFIFVMETYKEIINSCSIKQGYNMEQILLPKWIRQLE 548
FT++V+R LS+ + +++KICF+ T+ EI QG ++ + W QLE
Sbjct: 611 LFTESVKRWDLSLVDQMPQQMKICFVGFYNTFNEIAKEGRESQGRDVLGYIQNVWKVQLE 670
Query: 547 AFELEATWFTEREIPSMEVYLRNGCVSVSVWPIFTIARLLTCKFSCHYKDLKNELKINTE 368
A+ EA W + +PS Y+ N VS+++ + I+ L T + ++ +++ +
Sbjct: 671 AYTKEAEWSEAKYVPSFNEYIENASVSIALGTVVLISALFTGEVLT--DEVLSKIDRGSR 728
Query: 367 LYKLCAQSVRIHNDLLEELKEALVTKEISVIDCYMKERPLEKREDVTKALRSLVREHGKT 188
+L + R+ ND E + S I CYMK+ P E+ K + +++ +
Sbjct: 729 FLQLMGLTGRLVNDTKTYQAERGQGEVASAIQCYMKDHPKISEEEALKHVYTVMENSLEE 788
Query: 187 LSKLFMDPNGIEEYERVAFELTRALHFLYKRA*CFT-SYEGDKELNFKEC 41
L++ F++ + Y R+ FE R + Y + T S++ + + + K C
Sbjct: 789 LNREFVNNKIPDIYRRLVFETARIMQLFYMQGDGLTLSHDMEIKEHVKNC 838
>gb|AAK83563.1| abietadiene synthase [Abies grandis]
Length = 853
Score = 115 bits (287), Expect = 3e-23
Identities = 75/290 (25%), Positives = 143/290 (49%), Gaps = 1/290 (0%)
Frame = -3
Query: 907 SAVSKLKIFRQNLLESYMMVVLGNFEPELSDFRIILTKAIMTVFILDNIYEYQLNVNQVK 728
S ++L R+ + E Y FEPE S R + TK ILD++Y+ +++ +K
Sbjct: 560 SGFTELNFTRERVTEIYFSPASFIFEPEFSKCREVYTKTSNFTVILDDLYDAHGSLDDLK 619
Query: 727 EFTKAVRRQKLSMKETFSEEVKICFIFVMETYKEIINSCSIKQGYNMEQILLPKWIRQLE 548
FT++V+R LS+ + +++KICF+ T+ +I +QG ++ + W QLE
Sbjct: 620 LFTESVKRWDLSLVDQMPKQMKICFVGFYNTFNDIAKEGRERQGRDVLGYIQNVWKVQLE 679
Query: 547 AFELEATWFTEREIPSMEVYLRNGCVSVSVWPIFTIARLLTCKFSCHYKDLKNELKINTE 368
A+ EA W + +PS Y+ N VS+++ + I+ L T + ++ +++ +
Sbjct: 680 AYTKEAEWSEAKYVPSFNEYIENASVSIALGTVVLISALFTGEVLT--DEVLSKIDRESR 737
Query: 367 LYKLCAQSVRIHNDLLEELKEALVTKEISVIDCYMKERPLEKREDVTKALRSLVREHGKT 188
+L + R+ ND E + S I CYMK+ P E+ + + S++ +
Sbjct: 738 FLQLMGLTGRLVNDTKTYQAERGQGEVASAIQCYMKDHPKISEEEALQHVYSVMENALEE 797
Query: 187 LSKLFMDPNGIEEYERVAFELTRALHFLYKRA*CFT-SYEGDKELNFKEC 41
L++ F++ + Y+R+ FE R + Y + T S++ + + + K C
Sbjct: 798 LNREFVNNKIPDIYKRLVFETARIMQLFYMQGDGLTLSHDMEIKEHVKNC 847
>sp|Q38710.1|TPSDV_ABIGR RecName: Full=Abietadiene synthase, chloroplastic; AltName:
Full=(-)-abieta-7(8),13(14)-diene synthase; AltName:
Full=Abietadiene cyclase; Short=AgAS; AltName:
Full=Agggabi; Includes: RecName: Full=Abietadiene
synthase; AltName: Full=Neoabietadiene synthase;
Includes: RecName: Full=Copalyl diphosphate synthase;
Flags: Precursor gi|1477584|gb|AAB05407.1| abietadiene
cyclase [Abies grandis]
Length = 868
Score = 114 bits (286), Expect = 4e-23
Identities = 75/290 (25%), Positives = 142/290 (48%), Gaps = 1/290 (0%)
Frame = -3
Query: 907 SAVSKLKIFRQNLLESYMMVVLGNFEPELSDFRIILTKAIMTVFILDNIYEYQLNVNQVK 728
S + L R+ + E Y FEPE S R + TK ILD++Y+ +++ +K
Sbjct: 575 SGFTDLNFTRERVTEIYFSPASFIFEPEFSKCREVYTKTSNFTVILDDLYDAHGSLDDLK 634
Query: 727 EFTKAVRRQKLSMKETFSEEVKICFIFVMETYKEIINSCSIKQGYNMEQILLPKWIRQLE 548
FT++V+R LS+ + +++KICF+ T+ +I +QG ++ + W QLE
Sbjct: 635 LFTESVKRWDLSLVDQMPQQMKICFVGFYNTFNDIAKEGRERQGRDVLGYIQNVWKVQLE 694
Query: 547 AFELEATWFTEREIPSMEVYLRNGCVSVSVWPIFTIARLLTCKFSCHYKDLKNELKINTE 368
A+ EA W + +PS Y+ N VS+++ + I+ L T + ++ +++ +
Sbjct: 695 AYTKEAEWSEAKYVPSFNEYIENASVSIALGTVVLISALFTGEVLT--DEVLSKIDRESR 752
Query: 367 LYKLCAQSVRIHNDLLEELKEALVTKEISVIDCYMKERPLEKREDVTKALRSLVREHGKT 188
+L + R+ ND E + S I CYMK+ P E+ + + S++ +
Sbjct: 753 FLQLMGLTGRLVNDTKTYQAERGQGEVASAIQCYMKDHPKISEEEALQHVYSVMENALEE 812
Query: 187 LSKLFMDPNGIEEYERVAFELTRALHFLYKRA*CFT-SYEGDKELNFKEC 41
L++ F++ + Y+R+ FE R + Y + T S++ + + + K C
Sbjct: 813 LNREFVNNKIPDIYKRLVFETARIMQLFYMQGDGLTLSHDMEIKEHVKNC 862
>pdb|3S9V|A Chain A, Abietadiene Synthase From Abies Grandis
gi|371927470|pdb|3S9V|B Chain B, Abietadiene Synthase
From Abies Grandis gi|371927471|pdb|3S9V|C Chain C,
Abietadiene Synthase From Abies Grandis
gi|371927472|pdb|3S9V|D Chain D, Abietadiene Synthase
From Abies Grandis
Length = 785
Score = 114 bits (286), Expect = 4e-23
Identities = 75/290 (25%), Positives = 142/290 (48%), Gaps = 1/290 (0%)
Frame = -3
Query: 907 SAVSKLKIFRQNLLESYMMVVLGNFEPELSDFRIILTKAIMTVFILDNIYEYQLNVNQVK 728
S + L R+ + E Y FEPE S R + TK ILD++Y+ +++ +K
Sbjct: 492 SGFTDLNFTRERVTEIYFSPASFIFEPEFSKCREVYTKTSNFTVILDDLYDAHGSLDDLK 551
Query: 727 EFTKAVRRQKLSMKETFSEEVKICFIFVMETYKEIINSCSIKQGYNMEQILLPKWIRQLE 548
FT++V+R LS+ + +++KICF+ T+ +I +QG ++ + W QLE
Sbjct: 552 LFTESVKRWDLSLVDQMPQQMKICFVGFYNTFNDIAKEGRERQGRDVLGYIQNVWKVQLE 611
Query: 547 AFELEATWFTEREIPSMEVYLRNGCVSVSVWPIFTIARLLTCKFSCHYKDLKNELKINTE 368
A+ EA W + +PS Y+ N VS+++ + I+ L T + ++ +++ +
Sbjct: 612 AYTKEAEWSEAKYVPSFNEYIENASVSIALGTVVLISALFTGEVLT--DEVLSKIDRESR 669
Query: 367 LYKLCAQSVRIHNDLLEELKEALVTKEISVIDCYMKERPLEKREDVTKALRSLVREHGKT 188
+L + R+ ND E + S I CYMK+ P E+ + + S++ +
Sbjct: 670 FLQLMGLTGRLVNDTKTYQAERGQGEVASAIQCYMKDHPKISEEEALQHVYSVMENALEE 729
Query: 187 LSKLFMDPNGIEEYERVAFELTRALHFLYKRA*CFT-SYEGDKELNFKEC 41
L++ F++ + Y+R+ FE R + Y + T S++ + + + K C
Sbjct: 730 LNREFVNNKIPDIYKRLVFETARIMQLFYMQGDGLTLSHDMEIKEHVKNC 779
>gb|ADZ45517.1| levopimaradiene/abietadiene synthase [Picea sitchensis]
Length = 859
Score = 113 bits (283), Expect = 9e-23
Identities = 76/291 (26%), Positives = 140/291 (48%), Gaps = 2/291 (0%)
Frame = -3
Query: 907 SAVSKLKIFRQNLLESYMMVVLGNFEPELSDFRIILTKAIMTVFILDNIYEYQLNVNQVK 728
S ++LK R+ + E Y FEPE + R + TK ILD++Y+ ++ +K
Sbjct: 565 SGFTELKFTRERVTEIYFSPASFMFEPEFATCRAVYTKTSNFTVILDDLYDAHGTLDDLK 624
Query: 727 EFTKAVRRQKLSMKETFSEEVKICFIFVMETYKEIINSCSIKQGYNMEQILLPKWIRQLE 548
F+ +V++ LS+ + E++KICF+ T+ EI +QG ++ + W QLE
Sbjct: 625 LFSDSVKKWDLSLVDRMPEDMKICFMGFYNTFNEIAEEGRKRQGRDVLGYIRNVWEIQLE 684
Query: 547 AFELEATWFTEREIPSMEVYLRNGCVSVSVWPIFTIARLLTCKFSCHYKDLKNELKINTE 368
A+ EA W R +PS + Y+ N VS+++ + I+ L T + D+ +++ +
Sbjct: 685 AYTKEAEWSAARYVPSFDEYIENASVSIALGTVVLISALFTGEILT--DDVLSKIGRGSR 742
Query: 367 LYKLCAQSVRIHNDLLEELKEALVTKEISVIDCYMKERPLEKREDVTKALRSLVREHGKT 188
+L + R+ ND E + S + CYMK+ P E+ K + +++
Sbjct: 743 FLQLMGLTGRLVNDTKTYEAERGQGEVASAVQCYMKDHPEISEEEALKHVYTVMENALDE 802
Query: 187 LSKLFMDPNGI-EEYERVAFELTRALHFLYKRA*CFT-SYEGDKELNFKEC 41
L++ F++ + + R+ FE R + Y T S+E + + + K C
Sbjct: 803 LNREFVNNREVPDSCRRLVFETARIMQLFYMDGDGLTLSHETEIKEHVKNC 853
>gb|ACN40849.1| unknown [Picea sitchensis]
Length = 866
Score = 113 bits (283), Expect = 9e-23
Identities = 76/291 (26%), Positives = 140/291 (48%), Gaps = 2/291 (0%)
Frame = -3
Query: 907 SAVSKLKIFRQNLLESYMMVVLGNFEPELSDFRIILTKAIMTVFILDNIYEYQLNVNQVK 728
S ++LK R+ + E Y FEPE + R + TK ILD++Y+ ++ +K
Sbjct: 572 SGFTELKFTRERVTEIYFSPASFMFEPEFATCRAVYTKTSNFTVILDDLYDAHGTLDDLK 631
Query: 727 EFTKAVRRQKLSMKETFSEEVKICFIFVMETYKEIINSCSIKQGYNMEQILLPKWIRQLE 548
F+ +V++ LS+ + E++KICF+ T+ EI +QG ++ + W QLE
Sbjct: 632 LFSDSVKKWDLSLVDRMPEDMKICFMGFYNTFNEIAEEGRKRQGRDVLGYIRNVWEIQLE 691
Query: 547 AFELEATWFTEREIPSMEVYLRNGCVSVSVWPIFTIARLLTCKFSCHYKDLKNELKINTE 368
A+ EA W R +PS + Y+ N VS+++ + I+ L T + D+ +++ +
Sbjct: 692 AYTKEAEWSAARYVPSFDEYIENASVSIALGTVVLISALFTGEILT--DDVLSKIGRGSR 749
Query: 367 LYKLCAQSVRIHNDLLEELKEALVTKEISVIDCYMKERPLEKREDVTKALRSLVREHGKT 188
+L + R+ ND E + S + CYMK+ P E+ K + +++
Sbjct: 750 FLQLMGLTGRLVNDTKTYEAERGQGEVASAVQCYMKDHPEISEEEALKHVYTVMENALDE 809
Query: 187 LSKLFMDPNGI-EEYERVAFELTRALHFLYKRA*CFT-SYEGDKELNFKEC 41
L++ F++ + + R+ FE R + Y T S+E + + + K C
Sbjct: 810 LNREFVNNREVPDSCRRLVFETARIMQLFYMDGDGLTLSHETEIKEHVKNC 860
>gb|ABV44452.1| caryophyllene/humulene synthase [Pinus sylvestris]
Length = 575
Score = 112 bits (280), Expect = 2e-22
Identities = 69/276 (25%), Positives = 138/276 (50%), Gaps = 6/276 (2%)
Frame = -3
Query: 907 SAVSKLKIFRQNLLESYMMVVLGNFEPELSDFRIILTKAIMTVFILDNIYEYQLNVNQVK 728
S V+++ +R+ +E Y V+ FEPE S RI K +LD++Y+ ++++K
Sbjct: 279 SEVAQVNFYRRRHMELYFWAVISIFEPEFSQSRIAFAKVTTVGTVLDDLYDTYGMLDELK 338
Query: 727 EFTKAVRRQKLSMKETFSEEVKICFIFVMETYKEIINSCSIKQGYNMEQILLPKWIRQLE 548
T+ VRR +S+ + E++KI F T E+ KQG + IL W+R LE
Sbjct: 339 TITEGVRRWDISLIDDLPEKIKIAIQFFFNTANELAAEVVSKQGPDTSAILKDTWVRYLE 398
Query: 547 AFELEATWFTEREIPSMEVYLRNGCVS-----VSVWPIFTIARLLTCKFSCHYKDLKNEL 383
++ EA W T +P+ Y++N S V++ P+ + + L ++ ++
Sbjct: 399 SYLQEAEWITTGCVPTFSEYIKNAVASSGMCIVNLIPLLLMGQPLP-------NNILQQI 451
Query: 382 KINTELYKLCAQSVRIHNDLLEELKEALVTKEISVIDCYMKERPLEKREDVTKALRSLVR 203
+++ +L ++R+ +DL + E + S+I+CY+K+ P + ++ +++
Sbjct: 452 HSPSKIQELSELTIRLIDDLRDFEDEKERGEMASIIECYIKDNPDSTVGNALNRIKGILQ 511
Query: 202 EHGKTLSKLFMDPNGIEE-YERVAFELTRALHFLYK 98
+ +++ F+ + + ++ F +TR L FLYK
Sbjct: 512 LSLEEMNREFLKQDSVPLCCKKFTFNITRGLQFLYK 547
>sp|Q84LB2.2|AFS1_MALDO RecName: Full=(E,E)-alpha-farnesene synthase; Short=MdASF1; AltName:
Full=Prenyltransferase gi|32265058|gb|AAO22848.2|
(E,E)-alpha-farnesene synthase [Malus domestica]
Length = 576
Score = 112 bits (280), Expect = 2e-22
Identities = 95/308 (30%), Positives = 139/308 (45%), Gaps = 7/308 (2%)
Frame = -3
Query: 913 NQSAVSKLKIFRQNLLESYMMVVLGNFEPELSDFRIILTKAIMTVFILDNIYEYQLNVNQ 734
N LK R L+E + V FEPE S FRI LTK I V I+D++Y+ + +
Sbjct: 278 NLGIADNLKFARDRLVECFACAVGVAFEPEHSSFRICLTKVINLVLIIDDVYDIYGSEEE 337
Query: 733 VKEFTKAVRRQKLSMKETFSEEVKICFIFVMETYKEIINSCSIKQGYNMEQILLPK-WIR 557
+K FT AV R E E +K+CF + T EI + G+N L K W
Sbjct: 338 LKHFTNAVDRWDSRETEQLPECMKMCFQVLYNTTCEIAREIEEENGWNQVLPQLTKVWAD 397
Query: 556 QLEAFELEATWFTEREIPSMEVYLRNGCVSVSVWPIFTIARLLTCKFSCHY---KDLKNE 386
+A +EA W+ + IP++E YLRNGC+S SV + L+ FS + K++ +
Sbjct: 398 FCKALLVEAEWYNKSHIPTLEEYLRNGCISSSVSVL-----LVHSFFSITHEGTKEMADF 452
Query: 385 LKINTELYKLCAQSVRIHNDLLEELKEALVTKEISVIDCYMKERPLEKREDVTKALRSLV 206
L N +L + VR++NDL E S I CYM+E + E K ++ ++
Sbjct: 453 LHKNEDLLYNISLIVRLNNDLGTSAAEQERGDSPSSIVCYMREVNASE-ETARKNIKGMI 511
Query: 205 REHGKTLSKLFMDPNGI---EEYERVAFELTRALHFLYKRA*CFTSYEGDKELNFKECAH 35
K ++ N + + A + R H LYK F GD+E +
Sbjct: 512 DNAWKKVNGKCFTTNQVPFLSSFMNNATNMARVAHSLYKDGDGF----GDQEKGPRTHIL 567
Query: 34 RLFLQELV 11
L Q LV
Sbjct: 568 SLLFQPLV 575
>gb|AFU73863.1| levopimaradiene/abietadiene synthase [Pinus contorta]
Length = 850
Score = 112 bits (279), Expect = 3e-22
Identities = 72/272 (26%), Positives = 131/272 (48%), Gaps = 1/272 (0%)
Frame = -3
Query: 913 NQSAVSKLKIFRQNLLESYMMVVLGNFEPELSDFRIILTKAIMTVFILDNIYEYQLNVNQ 734
N S S+L R+ + E Y FEPE + R + TK ILD++Y+ ++
Sbjct: 554 NSSGFSQLGFTRERVAEIYFSPASFLFEPEFATCRAVYTKTSNFTVILDDLYDAHGTLDN 613
Query: 733 VKEFTKAVRRQKLSMKETFSEEVKICFIFVMETYKEIINSCSIKQGYNMEQILLPKWIRQ 554
+K F+++V+R LS+ + +++KICF T+ EI +QG ++ + W Q
Sbjct: 614 LKLFSESVKRWDLSLVDQMPQDMKICFKGFYNTFNEIAEEGRKRQGRDVLSYIQKVWEVQ 673
Query: 553 LEAFELEATWFTEREIPSMEVYLRNGCVSVSVWPIFTIARLLTCKFSCHYKDLKNELKIN 374
LEA+ EA W R +PS + Y+ N VS+++ + I+ L T + D+ +++ +
Sbjct: 674 LEAYTKEAEWSAVRYVPSYDEYIENASVSIALGTVVLISALFTGEILT--DDILSKIGRD 731
Query: 373 TELYKLCAQSVRIHNDLLEELKEALVTKEISVIDCYMKERPLEKREDVTKALRSLVREHG 194
+ L + R+ ND E + S + CYMK+ P E+ K + +++
Sbjct: 732 SRFLYLMGLTGRLVNDTKTYQAERGQGEVASAVQCYMKDHPEISEEEALKHVYTIMDNAL 791
Query: 193 KTLSKLFMDPNGI-EEYERVAFELTRALHFLY 101
L++ F++ + + R+ FE R + Y
Sbjct: 792 DELNREFVNNRDVPDTCRRLVFETARIMQLFY 823
>gb|AFU73862.1| levopimaradiene/abietadiene synthase [Pinus contorta]
Length = 857
Score = 112 bits (279), Expect = 3e-22
Identities = 72/272 (26%), Positives = 131/272 (48%), Gaps = 1/272 (0%)
Frame = -3
Query: 913 NQSAVSKLKIFRQNLLESYMMVVLGNFEPELSDFRIILTKAIMTVFILDNIYEYQLNVNQ 734
N S S+L R+ + E Y FEPE + R + TK ILD++Y+ ++
Sbjct: 561 NSSGFSQLGFTRERVAEIYFSPASFLFEPEFATCRAVYTKTSNFTVILDDLYDAHGTLDN 620
Query: 733 VKEFTKAVRRQKLSMKETFSEEVKICFIFVMETYKEIINSCSIKQGYNMEQILLPKWIRQ 554
+K F+++V+R LS+ + +++KICF T+ EI +QG ++ + W Q
Sbjct: 621 LKLFSESVKRWDLSLVDQMPQDMKICFKGFYNTFNEIAEEGRKRQGRDVLSYIQKVWEVQ 680
Query: 553 LEAFELEATWFTEREIPSMEVYLRNGCVSVSVWPIFTIARLLTCKFSCHYKDLKNELKIN 374
LEA+ EA W R +PS + Y+ N VS+++ + I+ L T + D+ +++ +
Sbjct: 681 LEAYTKEAEWSAVRYVPSYDEYIENASVSIALGTVVLISALFTGEILT--DDILSKIGRD 738
Query: 373 TELYKLCAQSVRIHNDLLEELKEALVTKEISVIDCYMKERPLEKREDVTKALRSLVREHG 194
+ L + R+ ND E + S + CYMK+ P E+ K + +++
Sbjct: 739 SRFLYLMGLTGRLVNDTKTYQAERGQGEVASAVQCYMKDHPEISEEEALKHVYTIMDNAL 798
Query: 193 KTLSKLFMDPNGI-EEYERVAFELTRALHFLY 101
L++ F++ + + R+ FE R + Y
Sbjct: 799 DELNREFVNNRDVPDTCRRLVFETARIMQLFY 830