BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Ephedra25_contig00008756
         (1987 letters)

Database: ./nr 
           37,332,560 sequences; 13,225,080,153 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_006470603.1| PREDICTED: mitochondrial tRNA-specific 2-thi...   488   e-135
ref|XP_002513510.1| tRNA (5-methylaminomethyl-2-thiouridylate)-m...   483   e-133
emb|CBI16383.3| unnamed protein product [Vitis vinifera]              480   e-133
ref|XP_002282827.1| PREDICTED: tRNA-specific 2-thiouridylase mnm...   480   e-133
ref|XP_006850208.1| hypothetical protein AMTR_s00022p00251260 [A...   480   e-132
ref|XP_004142116.1| PREDICTED: tRNA-specific 2-thiouridylase Mnm...   479   e-132
ref|XP_006358437.1| PREDICTED: mitochondrial tRNA-specific 2-thi...   479   e-132
ref|XP_002298404.2| hypothetical protein POPTR_0001s26620g [Popu...   479   e-132
gb|EXB93298.1| tRNA-specific 2-thiouridylase mnmA [Morus notabilis]   478   e-132
ref|XP_006307321.1| hypothetical protein CARUB_v10008942mg [Caps...   477   e-132
ref|XP_006280360.1| hypothetical protein CARUB_v10026287mg [Caps...   477   e-132
ref|XP_004247484.1| PREDICTED: tRNA-specific 2-thiouridylase Mnm...   476   e-131
ref|XP_006393065.1| hypothetical protein EUTSA_v10011432mg [Eutr...   473   e-130
dbj|BAD95081.1| hypothetical protein [Arabidopsis thaliana]           473   e-130
ref|XP_004507093.1| PREDICTED: tRNA-specific 2-thiouridylase Mnm...   472   e-130
ref|XP_004301461.1| PREDICTED: tRNA-specific 2-thiouridylase Mnm...   472   e-130
ref|XP_002894309.1| tRNA (5-methylaminomethyl-2-thiouridylate)-m...   472   e-130
ref|NP_175542.2| tRNA (5-methylaminomethyl-2-thiouridylate)-meth...   472   e-130
ref|XP_003539065.2| PREDICTED: mitochondrial tRNA-specific 2-thi...   468   e-129
ref|XP_002468413.1| hypothetical protein SORBIDRAFT_01g045520 [S...   468   e-129

>ref|XP_006470603.1| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1-like [Citrus
            sinensis]
          Length = 525

 Score =  488 bits (1256), Expect = e-135
 Identities = 245/450 (54%), Positives = 301/450 (66%), Gaps = 3/450 (0%)
 Frame = +1

Query: 343  DEKYLQCCWPGGVKRPLKIXXXXXXXXXXXXXXXXXXXXXHHCTAFYLKIDQDYVYENYM 522
            + +YL C  P   ++ LK+                     H CTAFYLKI     +EN+ 
Sbjct: 56   EPRYLSCSMPH--QKGLKVAVLLSGGVDSSVALRLLHSAGHSCTAFYLKIWFQEDFENFW 113

Query: 523  PGCPWEDDLKYVRSVCEQAGVDLQRIDFTDRYWKSVVSYMISEYKRGFTPNPDILCNTRI 702
              CPWE+D+KY ++VC+Q  V L+ +  TD YWK+VVSY+I EY+ G TPNPD+LCNTRI
Sbjct: 114  TECPWEEDMKYAKAVCDQVDVPLEVVHLTDEYWKNVVSYIIEEYRCGRTPNPDVLCNTRI 173

Query: 703  KFGEFLDYIDTMNFDYIASGHYARVQHSGTTSKDTSAILCLSLDNVKDQTYFXXXXXXXX 882
            KFG F+D I +M FDY+ASGHYA+V HS    +   ++L LS D VKDQTYF        
Sbjct: 174  KFGAFMDAISSMEFDYVASGHYAKVVHSSADQEYKPSVLELSKDKVKDQTYFLSHLSQAQ 233

Query: 883  XXXXIFPLGCIPKNEVRELAADMGLPNKDRKDSQGICFLGKVKFSEFVARHIGEMEGILL 1062
                IFPLGCI K EVR+ A    LPNKDRKDSQGICFLGK+KFSEFVARHIGEMEG++L
Sbjct: 234  LKRLIFPLGCISKEEVRKFATQFDLPNKDRKDSQGICFLGKIKFSEFVARHIGEMEGVIL 293

Query: 1063 EAETGAFLGNHRGFWFYTIGQRQGLGLTGGPWYVVAKDVENNVIFVSRNYYSLEMKRSFF 1242
            EAETG FLG H GFWFYTIGQRQGL L GGPWYVV KDV+NNV+FVSRNYYS + KR  F
Sbjct: 294  EAETGDFLGKHWGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYYSFDKKRRLF 353

Query: 1243 HVSNIRWIYA-TPEDFENIKCKVRHGPGFYDCK--IEHAKDIYWKEHNLPHPVEDSTQEV 1413
             V +++W+    PE+   ++CKVRHGPGFY+C   IEH              VED  + +
Sbjct: 354  RVGSLKWLSGLPPEEISRLQCKVRHGPGFYNCNLMIEH--------------VEDGGENI 399

Query: 1414 YTVTLSENDQGLAAGQFAAFYKDNICLGSGIIVGSSKDAYFPVCSKALEIAKIKDKSVLG 1593
              V LSE+DQGLAAGQFAAFY+   C+GSG+I+ S  D  FPVC KALEIA+++DKS LG
Sbjct: 400  AVVQLSEDDQGLAAGQFAAFYQGTRCIGSGVILESWDDQGFPVCEKALEIARMEDKSKLG 459

Query: 1594 KPIKIGTTVKSTETKNKFPAERAVKGSKEM 1683
            KPIKI       ET  +F + + V+ S+ +
Sbjct: 460  KPIKIKV---KPETPEEFNSNKGVQSSRNL 486


>ref|XP_002513510.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase,
            putative [Ricinus communis] gi|223547418|gb|EEF48913.1|
            tRNA
            (5-methylaminomethyl-2-thiouridylate)-methyltransferase,
            putative [Ricinus communis]
          Length = 497

 Score =  483 bits (1242), Expect = e-133
 Identities = 243/450 (54%), Positives = 299/450 (66%), Gaps = 5/450 (1%)
 Frame = +1

Query: 343  DEKYLQCCWPGGVKRPLKIXXXXXXXXXXXXXXXXXXXXXHHCTAFYLKIDQDYVYENYM 522
            +  YL CC P   K  L+I                     HHCTAFYLKI     +EN+ 
Sbjct: 57   EPNYLSCCLPD--KNNLRIALLLSGGVDSSVALRLLHAAGHHCTAFYLKIWFQEDFENFW 114

Query: 523  PGCPWEDDLKYVRSVCEQAGVDLQRIDFTDRYWKSVVSYMISEYKRGFTPNPDILCNTRI 702
              CPWE+DLKY ++VC+Q  + L+ +  TD YWK+VVSY+I EY+ G TPNPD+LCNTRI
Sbjct: 115  SECPWEEDLKYAKAVCDQVDIPLEVVHLTDEYWKNVVSYIIEEYRCGRTPNPDVLCNTRI 174

Query: 703  KFGEFLDYIDTMNFDYIASGHYARVQHSGTTSKDTSAILCLSLDNVKDQTYFXXXXXXXX 882
            KFG F+D I+ M FDY+ASGHYA+V H      +  ++L LS D VKDQTYF        
Sbjct: 175  KFGAFMDAINNMEFDYVASGHYAKVIHPSADEINQPSVLELSKDMVKDQTYFLSHLSQAQ 234

Query: 883  XXXXIFPLGCIPKNEVRELAADMGLPNKDRKDSQGICFLGKVKFSEFVARHIGEMEGILL 1062
                IFPLG I K+EVR+LA    LPNKDRKDSQGICFLGK+KFSEFVARHIGEMEGI+L
Sbjct: 235  LRRLIFPLGGISKDEVRKLATLFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEMEGIML 294

Query: 1063 EAETGAFLGNHRGFWFYTIGQRQGLGLTGGPWYVVAKDVENNVIFVSRNYYSLEMKRSFF 1242
            EAETG FLGNHRGFWFYTIGQRQGL L GGPWYVV KDV+NNV+FVSRNY+S + +R   
Sbjct: 295  EAETGDFLGNHRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSFDKRRRLL 354

Query: 1243 HVSNIRWIYAT-PEDFENIKCKVRHGPGFYDCKIEHAKDIYWKEHNLPHPVEDSTQEVYT 1419
             V ++RW   + P++   ++CKVRHGPGFY+C +            +  P E   Q+V  
Sbjct: 355  RVGSLRWFNGSPPKETSELQCKVRHGPGFYNCSV------------VIEPGEVDDQDVAI 402

Query: 1420 VTLSENDQGLAAGQFAAFYKDNICLGSGIIVGSSKDAYFPVCSKALEIAKIKDKSVLGKP 1599
            V L E+DQGLAAGQFAAFYK   C+GSG+I+ S  D  FPVC +ALE+A+I+DKS LGKP
Sbjct: 403  VQLCEDDQGLAAGQFAAFYKGRTCIGSGVILESWNDQGFPVCERALELARIEDKSELGKP 462

Query: 1600 IKIGT----TVKSTETKNKFPAERAVKGSK 1677
            +KI       V+  + K+    ER +  S+
Sbjct: 463  VKIKVKPEIPVREFDGKDGIEVERGLNNSQ 492


>emb|CBI16383.3| unnamed protein product [Vitis vinifera]
          Length = 544

 Score =  480 bits (1236), Expect = e-133
 Identities = 240/422 (56%), Positives = 285/422 (67%), Gaps = 1/422 (0%)
 Frame = +1

Query: 346  EKYLQCCWPGGVKRPLKIXXXXXXXXXXXXXXXXXXXXXHHCTAFYLKIDQDYVYENYMP 525
            + YL C  P      LKI                     H CTAFYLKI     +EN+  
Sbjct: 76   QPYLSCSLPNN---RLKIAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWS 132

Query: 526  GCPWEDDLKYVRSVCEQAGVDLQRIDFTDRYWKSVVSYMISEYKRGFTPNPDILCNTRIK 705
             CPWEDDLKY ++VC+Q  V L+ +  TD YWK+VVSY+I EY+ G TPNPD+LCNTRIK
Sbjct: 133  ECPWEDDLKYAKAVCDQVDVPLEVVHLTDEYWKNVVSYIIEEYRCGRTPNPDVLCNTRIK 192

Query: 706  FGEFLDYIDTMNFDYIASGHYARVQHSGTTSKDTSAILCLSLDNVKDQTYFXXXXXXXXX 885
            FG F+D I +  FDY+ASGHYA V HS T   D  ++L LS D VKDQTYF         
Sbjct: 193  FGAFMDAISSKEFDYVASGHYANVVHSFTDQMDKPSVLELSKDMVKDQTYFLSHLSQIQL 252

Query: 886  XXXIFPLGCIPKNEVRELAADMGLPNKDRKDSQGICFLGKVKFSEFVARHIGEMEGILLE 1065
               IFPLGCIPK+EVR LA  + LPN+DRKDSQGICFLGK+KFSEFVARHIGE EGI+LE
Sbjct: 253  KRLIFPLGCIPKDEVRSLATKLDLPNRDRKDSQGICFLGKIKFSEFVARHIGEKEGIILE 312

Query: 1066 AETGAFLGNHRGFWFYTIGQRQGLGLTGGPWYVVAKDVENNVIFVSRNYYSLEMKRSFFH 1245
            AETG  LG HRGFWFYTIGQRQGL L GGPWYVV KDV+NNV+FVSRNY+SL+ +R  F 
Sbjct: 313  AETGDLLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSLDKRRRLFR 372

Query: 1246 VSNIRWIYATPE-DFENIKCKVRHGPGFYDCKIEHAKDIYWKEHNLPHPVEDSTQEVYTV 1422
            V +++WI   P      ++CKVRHGPGFY+C                 P ED  ++V  V
Sbjct: 373  VGSLKWISREPPGQINQLQCKVRHGPGFYNCSF------------TMEPGEDGHEDVAVV 420

Query: 1423 TLSENDQGLAAGQFAAFYKDNICLGSGIIVGSSKDAYFPVCSKALEIAKIKDKSVLGKPI 1602
             LSE+DQGLAAGQF AFY+   C+GSG+I+ S  D  FPVC+KALEIA+++DKS LGKP+
Sbjct: 421  QLSEDDQGLAAGQFTAFYQGRSCIGSGVILESWDDQGFPVCAKALEIARMEDKSKLGKPV 480

Query: 1603 KI 1608
            KI
Sbjct: 481  KI 482


>ref|XP_002282827.1| PREDICTED: tRNA-specific 2-thiouridylase mnmA-like [Vitis vinifera]
          Length = 540

 Score =  480 bits (1236), Expect = e-133
 Identities = 240/422 (56%), Positives = 285/422 (67%), Gaps = 1/422 (0%)
 Frame = +1

Query: 346  EKYLQCCWPGGVKRPLKIXXXXXXXXXXXXXXXXXXXXXHHCTAFYLKIDQDYVYENYMP 525
            + YL C  P      LKI                     H CTAFYLKI     +EN+  
Sbjct: 72   QPYLSCSLPNN---RLKIAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWS 128

Query: 526  GCPWEDDLKYVRSVCEQAGVDLQRIDFTDRYWKSVVSYMISEYKRGFTPNPDILCNTRIK 705
             CPWEDDLKY ++VC+Q  V L+ +  TD YWK+VVSY+I EY+ G TPNPD+LCNTRIK
Sbjct: 129  ECPWEDDLKYAKAVCDQVDVPLEVVHLTDEYWKNVVSYIIEEYRCGRTPNPDVLCNTRIK 188

Query: 706  FGEFLDYIDTMNFDYIASGHYARVQHSGTTSKDTSAILCLSLDNVKDQTYFXXXXXXXXX 885
            FG F+D I +  FDY+ASGHYA V HS T   D  ++L LS D VKDQTYF         
Sbjct: 189  FGAFMDAISSKEFDYVASGHYANVVHSFTDQMDKPSVLELSKDMVKDQTYFLSHLSQIQL 248

Query: 886  XXXIFPLGCIPKNEVRELAADMGLPNKDRKDSQGICFLGKVKFSEFVARHIGEMEGILLE 1065
               IFPLGCIPK+EVR LA  + LPN+DRKDSQGICFLGK+KFSEFVARHIGE EGI+LE
Sbjct: 249  KRLIFPLGCIPKDEVRSLATKLDLPNRDRKDSQGICFLGKIKFSEFVARHIGEKEGIILE 308

Query: 1066 AETGAFLGNHRGFWFYTIGQRQGLGLTGGPWYVVAKDVENNVIFVSRNYYSLEMKRSFFH 1245
            AETG  LG HRGFWFYTIGQRQGL L GGPWYVV KDV+NNV+FVSRNY+SL+ +R  F 
Sbjct: 309  AETGDLLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSLDKRRRLFR 368

Query: 1246 VSNIRWIYATPE-DFENIKCKVRHGPGFYDCKIEHAKDIYWKEHNLPHPVEDSTQEVYTV 1422
            V +++WI   P      ++CKVRHGPGFY+C                 P ED  ++V  V
Sbjct: 369  VGSLKWISREPPGQINQLQCKVRHGPGFYNCSF------------TMEPGEDGHEDVAVV 416

Query: 1423 TLSENDQGLAAGQFAAFYKDNICLGSGIIVGSSKDAYFPVCSKALEIAKIKDKSVLGKPI 1602
             LSE+DQGLAAGQF AFY+   C+GSG+I+ S  D  FPVC+KALEIA+++DKS LGKP+
Sbjct: 417  QLSEDDQGLAAGQFTAFYQGRSCIGSGVILESWDDQGFPVCAKALEIARMEDKSKLGKPV 476

Query: 1603 KI 1608
            KI
Sbjct: 477  KI 478


>ref|XP_006850208.1| hypothetical protein AMTR_s00022p00251260 [Amborella trichopoda]
            gi|548853806|gb|ERN11789.1| hypothetical protein
            AMTR_s00022p00251260 [Amborella trichopoda]
          Length = 565

 Score =  480 bits (1235), Expect = e-132
 Identities = 245/439 (55%), Positives = 299/439 (68%), Gaps = 3/439 (0%)
 Frame = +1

Query: 301  CLFPQHEQKQWRPADEKYLQCCWPGGVKRPLKIXXXXXXXXXXXXXXXXXXXXXHHCTAF 480
            C  P++        +++YL+C  P   ++  K+                     H CTAF
Sbjct: 97   CFLPENHYSGSFVPEKRYLECSLP---EKRSKVAVLVSGGVDSSVALRLLCYAGHSCTAF 153

Query: 481  YLKIDQDYVYENYMPGCPWEDDLKYVRSVCEQAGVDLQRIDFTDRYWKSVVSYMISEYKR 660
            YLKI     +EN+   CPW++DLKY ++VC++  V L+ +  TD YW +VVS++ISEY+ 
Sbjct: 154  YLKIWFQEDFENFWSACPWDEDLKYAKAVCDEVHVPLEVVHLTDEYWNNVVSHIISEYRS 213

Query: 661  GFTPNPDILCNTRIKFGEFLDYIDTMNFDYIASGHYARVQHSGTTSKDTSAILCLSLDNV 840
            G TPNPD+LCNTRIKFG FLD I  M+FDY+ASGHYARV H      D S+ L LS D V
Sbjct: 214  GRTPNPDVLCNTRIKFGAFLDAIGNMDFDYVASGHYARVVHPPPDQPDASSALELSADKV 273

Query: 841  KDQTYFXXXXXXXXXXXXIFPLGCIPKNEVRELAADMGLPNKDRKDSQGICFLGKVKFSE 1020
            KDQTYF            +FPLGC+ K+EVR LAA MGLPNKDRKDSQGICFLGK+KFS+
Sbjct: 274  KDQTYFLSHLSQTQLNRLLFPLGCLSKDEVRRLAAKMGLPNKDRKDSQGICFLGKLKFSD 333

Query: 1021 FVARHIGEMEGILLEAETGAFLGNHRGFWFYTIGQRQGLGLTGGPWYVVAKDVENNVIFV 1200
            FV RHIGEMEG+LLEAETG FLG H+G+WFYTIGQRQGL L GGPWYVV KD+ENNVIFV
Sbjct: 334  FVTRHIGEMEGVLLEAETGDFLGKHQGYWFYTIGQRQGLRLPGGPWYVVNKDIENNVIFV 393

Query: 1201 SRNYYSLEMKRSFFHVSNIRWIY-ATPEDFENIKCKVRHGPGFYDC--KIEHAKDIYWKE 1371
            SRNY+S + KR  F V +++W+  A PE  + ++CKVRHGP FYDC  KIE   D    E
Sbjct: 394  SRNYFSFDKKRRSFRVGSLKWLSGAPPEHLDQLQCKVRHGPCFYDCTFKIESRCD----E 449

Query: 1372 HNLPHPVEDSTQEVYTVTLSENDQGLAAGQFAAFYKDNICLGSGIIVGSSKDAYFPVCSK 1551
            H      EDS      + +SE+DQGLAAGQFAAFY+ N+C+GSGII+ S  D  F VCSK
Sbjct: 450  H------EDSA----LIHISEDDQGLAAGQFAAFYRKNVCIGSGIILESWDDQNFSVCSK 499

Query: 1552 ALEIAKIKDKSVLGKPIKI 1608
            ALE AK++DKS LG PIKI
Sbjct: 500  ALERAKMEDKSKLGLPIKI 518


>ref|XP_004142116.1| PREDICTED: tRNA-specific 2-thiouridylase MnmA-like [Cucumis sativus]
            gi|449524848|ref|XP_004169433.1| PREDICTED: tRNA-specific
            2-thiouridylase MnmA-like [Cucumis sativus]
          Length = 538

 Score =  479 bits (1234), Expect = e-132
 Identities = 252/479 (52%), Positives = 309/479 (64%), Gaps = 19/479 (3%)
 Frame = +1

Query: 352  YLQCCWPGGVKRPLKIXXXXXXXXXXXXXXXXXXXXXHHCTAFYLKIDQDYVYENYMPGC 531
            +L C  P     PLK+                     H CTAFYLKI     +EN+   C
Sbjct: 72   FLSCSMP---TYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSEC 128

Query: 532  PWEDDLKYVRSVCEQAGVDLQRIDFTDRYWKSVVSYMISEYKRGFTPNPDILCNTRIKFG 711
            PW+DDLKY ++VC+Q  V L+ +  T+ YW++VVSY+I EY+ G TPNPD+LCNTRIKFG
Sbjct: 129  PWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFG 188

Query: 712  EFLDYIDTMNFDYIASGHYARVQHSGTTSKDTSAILCLSLDNVKDQTYFXXXXXXXXXXX 891
             F+D I  M FDY+ASGHYA V H      D  ++L LS D VKDQTYF           
Sbjct: 189  AFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLSQNQLKR 248

Query: 892  XIFPLGCIPKNEVRELAADMGLPNKDRKDSQGICFLGKVKFSEFVARHIGEMEGILLEAE 1071
             +FPLGCIPK+EVR+LAA   LPNKDRKDSQGICFLGK+KFSEFVARHIGE EG++LEAE
Sbjct: 249  LLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAE 308

Query: 1072 TGAFLGNHRGFWFYTIGQRQGLGLTGGPWYVVAKDVENNVIFVSRNYYSLEMKRSFFHVS 1251
            +G FLG HRGFWFYTIGQRQGL L GGPWYVV KD++NNV+FVSRNY+S + +R  F V 
Sbjct: 309  SGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRVFRVG 368

Query: 1252 NIRWIYATPED-FENIKCKVRHGPGFYDCKIEHAKDIYWKEHNLPHPVEDSTQEVYTVTL 1428
            +++W+   P D F  ++CKVRHGP FY C +         EH+     ED  ++V  V L
Sbjct: 369  SLKWLSGFPPDQFTELQCKVRHGPSFYSCNLT-------MEHS-----EDGHEDVAVVHL 416

Query: 1429 SENDQGLAAGQFAAFYKDNICLGSGIIVGSSKDAYFPVCSKALEIAKIKDKSVLGKPIKI 1608
            SE+DQGLAAGQFAAFYK   C+GSG+I+ S  D  FPVC KA+E A+++DKS LGKPIKI
Sbjct: 417  SEDDQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKI 476

Query: 1609 GTTVKS----------TETKNKFPAERA--VKGSKEMKPIH------MKKGGSLKKESL 1731
                +S          TE  +K   E A  V+  +E+ P        MK  GSLKK+ L
Sbjct: 477  KVKPESGMKESKAKDDTEIHDKLRNEEATVVEQIREVSPDEAMATPSMKWLGSLKKKLL 535


>ref|XP_006358437.1| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1-like
            [Solanum tuberosum]
          Length = 488

 Score =  479 bits (1233), Expect = e-132
 Identities = 240/427 (56%), Positives = 287/427 (67%), Gaps = 5/427 (1%)
 Frame = +1

Query: 343  DEK----YLQCCWPGGVKRPLKIXXXXXXXXXXXXXXXXXXXXXHHCTAFYLKIDQDYVY 510
            DEK    YL C  P   K PLK+                     H CTAFYLKI     +
Sbjct: 66   DEKINPTYLSCSMPH--KNPLKVAVLVSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDF 123

Query: 511  ENYMPGCPWEDDLKYVRSVCEQAGVDLQRIDFTDRYWKSVVSYMISEYKRGFTPNPDILC 690
            ENY   CPWEDDLKY ++VC+Q  V L+ +  TD YW +VVSY+I EYK G TPNPD+LC
Sbjct: 124  ENYWSECPWEDDLKYAKAVCDQVDVPLEVVHLTDEYWNNVVSYIIEEYKCGRTPNPDVLC 183

Query: 691  NTRIKFGEFLDYIDTMNFDYIASGHYARVQHSGTTSKDTSAILCLSLDNVKDQTYFXXXX 870
            NTRIKFG F+D I  M FD++ASGHYA++ H+ T   D  +IL LS D VKDQTYF    
Sbjct: 184  NTRIKFGAFMDAISGMEFDFVASGHYAKIVHASTAQLDEPSILKLSKDMVKDQTYFLSHL 243

Query: 871  XXXXXXXXIFPLGCIPKNEVRELAADMGLPNKDRKDSQGICFLGKVKFSEFVARHIGEME 1050
                    IFPLGCI K+EVR LA    LPN+DRKDSQGICFLGK+KFSEFVARHIGE E
Sbjct: 244  SQAQLKRLIFPLGCIQKDEVRTLAKSFNLPNQDRKDSQGICFLGKIKFSEFVARHIGESE 303

Query: 1051 GILLEAETGAFLGNHRGFWFYTIGQRQGLGLTGGPWYVVAKDVENNVIFVSRNYYSLEMK 1230
            GI+LEAE G +LGNHRGFWFYTIGQRQGL L GGPWYVV KD++NNV++VSRNY+S++ K
Sbjct: 304  GIILEAENGDYLGNHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVYVSRNYFSVDKK 363

Query: 1231 RSFFHVSNIRWIYAT-PEDFENIKCKVRHGPGFYDCKIEHAKDIYWKEHNLPHPVEDSTQ 1407
            R  F V +++W+    P+    ++CKVRHGP FYDC             +L   V+   Q
Sbjct: 364  RRLFRVGSLKWLSGLFPKQINELQCKVRHGPSFYDC-------------SLVMEVDQHGQ 410

Query: 1408 EVYTVTLSENDQGLAAGQFAAFYKDNICLGSGIIVGSSKDAYFPVCSKALEIAKIKDKSV 1587
            EV  V LS +DQGLAAGQFAAFY    C+GSGII+ S  D  +P+C KALEIA+++DKS 
Sbjct: 411  EVAVVRLSGDDQGLAAGQFAAFYNGRTCIGSGIILESWDDQGYPICEKALEIARMEDKSK 470

Query: 1588 LGKPIKI 1608
            LGKP+KI
Sbjct: 471  LGKPVKI 477


>ref|XP_002298404.2| hypothetical protein POPTR_0001s26620g [Populus trichocarpa]
            gi|550348245|gb|EEE83209.2| hypothetical protein
            POPTR_0001s26620g [Populus trichocarpa]
          Length = 512

 Score =  479 bits (1232), Expect = e-132
 Identities = 236/434 (54%), Positives = 292/434 (67%), Gaps = 1/434 (0%)
 Frame = +1

Query: 343  DEKYLQCCWPGGVKRPLKIXXXXXXXXXXXXXXXXXXXXXHHCTAFYLKIDQDYVYENYM 522
            D  YL CC     K PL+I                     HHCTAFYLKI     + N+ 
Sbjct: 71   DPYYLSCCMADKDK-PLRIAVLLSGGVDSSVALRLLHAAGHHCTAFYLKIWFQEDFNNFW 129

Query: 523  PGCPWEDDLKYVRSVCEQAGVDLQRIDFTDRYWKSVVSYMISEYKRGFTPNPDILCNTRI 702
              CPWE+DLK+ + VC+Q GV L+ +  TD YW +VVSY+I EY+ G TPNPD+LCNTRI
Sbjct: 130  SECPWEEDLKFAKGVCDQVGVPLEVVHLTDEYWSNVVSYIIEEYRCGRTPNPDVLCNTRI 189

Query: 703  KFGEFLDYIDTMNFDYIASGHYARVQHSGTTSKDTSAILCLSLDNVKDQTYFXXXXXXXX 882
            KFG F+D I+ M FDY+ASGHYA V H  +   +  ++L LS D +KDQTYF        
Sbjct: 190  KFGAFMDAINNMEFDYVASGHYAHVIHPVSDQINKPSVLELSKDKLKDQTYFLSHLTQAQ 249

Query: 883  XXXXIFPLGCIPKNEVRELAADMGLPNKDRKDSQGICFLGKVKFSEFVARHIGEMEGILL 1062
                IFPLGCIPK EVR LA+   LPNKDRKDSQGICFLGK+KFSEFV RHIGE EG++L
Sbjct: 250  LKRLIFPLGCIPKEEVRNLASKFDLPNKDRKDSQGICFLGKIKFSEFVGRHIGESEGVIL 309

Query: 1063 EAETGAFLGNHRGFWFYTIGQRQGLGLTGGPWYVVAKDVENNVIFVSRNYYSLEMKRSFF 1242
            EAETG FLG HRGFWFYTIGQRQGL L GGPWYVV KDV+NNV+FVSRNY+SL+ +R  F
Sbjct: 310  EAETGDFLGIHRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSLDKRRRSF 369

Query: 1243 HVSNIRWIYATPEDF-ENIKCKVRHGPGFYDCKIEHAKDIYWKEHNLPHPVEDSTQEVYT 1419
             V +++W+  +P D    ++CKVRHGP FYDC ++                EDS +EV  
Sbjct: 370  RVGSLKWLSGSPTDMTTQLQCKVRHGPTFYDCSVKIELG------------EDSPEEVVA 417

Query: 1420 VTLSENDQGLAAGQFAAFYKDNICLGSGIIVGSSKDAYFPVCSKALEIAKIKDKSVLGKP 1599
            V L E+DQGLAAGQF AFY+   C+GSG+I+ S  D  FPVC KALE+A+++DKS LGKP
Sbjct: 418  VHLCEDDQGLAAGQFTAFYQGRTCIGSGVILESWDDKGFPVCQKALELARLEDKSKLGKP 477

Query: 1600 IKIGTTVKSTETKN 1641
            +KI    ++++ ++
Sbjct: 478  VKIKVKPETSDQES 491


>gb|EXB93298.1| tRNA-specific 2-thiouridylase mnmA [Morus notabilis]
          Length = 580

 Score =  478 bits (1229), Expect = e-132
 Identities = 240/432 (55%), Positives = 288/432 (66%), Gaps = 1/432 (0%)
 Frame = +1

Query: 316  HEQKQWRPADEKYLQCCWPGGVKRPLKIXXXXXXXXXXXXXXXXXXXXXHHCTAFYLKID 495
            H  K +    E YL C  P    + L++                     H CTAFYLKI 
Sbjct: 108  HHSKSFDGDIEPYLSCSMP---HKRLRVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIW 164

Query: 496  QDYVYENYMPGCPWEDDLKYVRSVCEQAGVDLQRIDFTDRYWKSVVSYMISEYKRGFTPN 675
                +EN+   CPWE+DLKY ++VC Q  V L+ +  TD YWK+VVSY+I EY+ G TPN
Sbjct: 165  FQEDFENFWSECPWEEDLKYAKAVCSQIEVPLEVVHLTDEYWKNVVSYIIDEYRCGRTPN 224

Query: 676  PDILCNTRIKFGEFLDYIDTMNFDYIASGHYARVQHSGTTSKDTSAILCLSLDNVKDQTY 855
            PD+LCNTRIKFG F+D I +M FDY+ASGHYA V HS T  +  +++L LS D VKDQTY
Sbjct: 225  PDVLCNTRIKFGSFMDAISSMEFDYVASGHYANVVHSFTNERGDASVLELSQDMVKDQTY 284

Query: 856  FXXXXXXXXXXXXIFPLGCIPKNEVRELAADMGLPNKDRKDSQGICFLGKVKFSEFVARH 1035
            F            +FPLGCI K EVR+LA    LPNKDRKDSQGICFLGK+KFSEFVARH
Sbjct: 285  FLSHLSQAQLKRLVFPLGCISKEEVRKLAKKFDLPNKDRKDSQGICFLGKIKFSEFVARH 344

Query: 1036 IGEMEGILLEAETGAFLGNHRGFWFYTIGQRQGLGLTGGPWYVVAKDVENNVIFVSRNYY 1215
            IGE EGI+LEAETG FLG HRGFWFYTIGQRQGL L GGPWYVV KDV+NNV+F SRNY+
Sbjct: 345  IGEKEGIILEAETGDFLGMHRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFASRNYF 404

Query: 1216 SLEMKRSFFHVSNIRWIYATPEDFE-NIKCKVRHGPGFYDCKIEHAKDIYWKEHNLPHPV 1392
            S + +R  F V +++W+   P D    ++CKVRHGPGFY+C                   
Sbjct: 405  SFDKRRRIFRVGSLKWLNGLPPDQTCQLQCKVRHGPGFYNCSFTMESG------------ 452

Query: 1393 EDSTQEVYTVTLSENDQGLAAGQFAAFYKDNICLGSGIIVGSSKDAYFPVCSKALEIAKI 1572
            E+S ++V  V LSE+DQGLA GQFAAFY+   CLGSGII+ S  D  FPVC KALEIAK+
Sbjct: 453  ENSGEDVAVVQLSEDDQGLAVGQFAAFYQGKSCLGSGIILESWDDQGFPVCEKALEIAKM 512

Query: 1573 KDKSVLGKPIKI 1608
            +DKS+LGKP+KI
Sbjct: 513  EDKSLLGKPVKI 524


>ref|XP_006307321.1| hypothetical protein CARUB_v10008942mg [Capsella rubella]
            gi|482576032|gb|EOA40219.1| hypothetical protein
            CARUB_v10008942mg [Capsella rubella]
          Length = 496

 Score =  477 bits (1228), Expect = e-132
 Identities = 231/424 (54%), Positives = 287/424 (67%), Gaps = 1/424 (0%)
 Frame = +1

Query: 346  EKYLQCCWPGGVKRPLKIXXXXXXXXXXXXXXXXXXXXXHHCTAFYLKIDQDYVYENYMP 525
            ++YL C  P   ++PL++                     H CTAFYLKI     +EN+  
Sbjct: 69   DQYLSCSMP---EKPLRVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEGFENFWD 125

Query: 526  GCPWEDDLKYVRSVCEQAGVDLQRIDFTDRYWKSVVSYMISEYKRGFTPNPDILCNTRIK 705
             CPWEDDLKY + VCEQ GV L+ +  TD YW+ VVSY+I EY+ G TPNPD+LCNTRIK
Sbjct: 126  QCPWEDDLKYAKHVCEQVGVPLEVVHLTDEYWERVVSYLIEEYRCGRTPNPDVLCNTRIK 185

Query: 706  FGEFLDYIDTMNFDYIASGHYARVQHSGTTSKDTSAILCLSLDNVKDQTYFXXXXXXXXX 885
            FG F+D I  M +DY+ASGHYA+V H      D+S++L LS D VKDQTYF         
Sbjct: 186  FGAFMDAISDMEYDYVASGHYAKVVHPPADQNDSSSLLELSQDMVKDQTYFLSHLSQTQL 245

Query: 886  XXXIFPLGCIPKNEVRELAADMGLPNKDRKDSQGICFLGKVKFSEFVARHIGEMEGILLE 1065
               +FPLGC+ K+EVR+LA    LPNKDRKDSQGICFLGK+KFS+FV RHIGEMEG++LE
Sbjct: 246  KRLLFPLGCVKKDEVRKLATQFDLPNKDRKDSQGICFLGKIKFSDFVGRHIGEMEGVILE 305

Query: 1066 AETGAFLGNHRGFWFYTIGQRQGLGLTGGPWYVVAKDVENNVIFVSRNYYSLEMKRSFFH 1245
            AETG FLGNHRGFWFYTIGQRQGL L GGPWYVV KD +NNV+F SRNYYS++ +R  F 
Sbjct: 306  AETGDFLGNHRGFWFYTIGQRQGLRLPGGPWYVVEKDTKNNVVFASRNYYSIDKRRRVFR 365

Query: 1246 VSNIRWIYATPE-DFENIKCKVRHGPGFYDCKIEHAKDIYWKEHNLPHPVEDSTQEVYTV 1422
            V ++RW+   P  +   ++CKVRHGPGFY C  E                 ++  +V  V
Sbjct: 366  VGSLRWLSGKPSANVSELRCKVRHGPGFYSCSFE----------------METKGDVAVV 409

Query: 1423 TLSENDQGLAAGQFAAFYKDNICLGSGIIVGSSKDAYFPVCSKALEIAKIKDKSVLGKPI 1602
             L E+DQGLAAGQFAAFY+   C+GSG+I+ S  D  FPVC+KAL++A ++DK+ LGKP+
Sbjct: 410  HLDEDDQGLAAGQFAAFYEGTTCIGSGVILESWDDQCFPVCAKALQLAAMEDKTKLGKPV 469

Query: 1603 KIGT 1614
            KI T
Sbjct: 470  KIFT 473


>ref|XP_006280360.1| hypothetical protein CARUB_v10026287mg [Capsella rubella]
            gi|482549064|gb|EOA13258.1| hypothetical protein
            CARUB_v10026287mg [Capsella rubella]
          Length = 494

 Score =  477 bits (1227), Expect = e-132
 Identities = 232/424 (54%), Positives = 286/424 (67%), Gaps = 1/424 (0%)
 Frame = +1

Query: 346  EKYLQCCWPGGVKRPLKIXXXXXXXXXXXXXXXXXXXXXHHCTAFYLKIDQDYVYENYMP 525
            ++YL C  P   ++PL++                     H CTAFYLKI     +EN+  
Sbjct: 67   DQYLSCSMP---EKPLRVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEGFENFWN 123

Query: 526  GCPWEDDLKYVRSVCEQAGVDLQRIDFTDRYWKSVVSYMISEYKRGFTPNPDILCNTRIK 705
             CPWEDDLKY + VCEQ  V L+ +  TD YW+ VVSY+I EY+ G TPNPD+LCNTRIK
Sbjct: 124  QCPWEDDLKYAKHVCEQVNVPLEVVHLTDEYWEKVVSYIIEEYRCGRTPNPDVLCNTRIK 183

Query: 706  FGEFLDYIDTMNFDYIASGHYARVQHSGTTSKDTSAILCLSLDNVKDQTYFXXXXXXXXX 885
            FG F+D I  M +DY+ASGHYA+V H      D+S++L LS D VKDQTYF         
Sbjct: 184  FGAFMDAISDMEYDYVASGHYAKVVHPPADQNDSSSLLELSQDMVKDQTYFLSHLSQTQL 243

Query: 886  XXXIFPLGCIPKNEVRELAADMGLPNKDRKDSQGICFLGKVKFSEFVARHIGEMEGILLE 1065
               +FPLGC+ K+EVR+LA    LPNKDRKDSQGICFLGK+KFS+FV  HIGEMEG++LE
Sbjct: 244  KRLLFPLGCVKKDEVRKLATQFDLPNKDRKDSQGICFLGKIKFSDFVGTHIGEMEGVILE 303

Query: 1066 AETGAFLGNHRGFWFYTIGQRQGLGLTGGPWYVVAKDVENNVIFVSRNYYSLEMKRSFFH 1245
            AETG FLGNHRGFWFYTIGQRQGL L GGPWYVV KD +NNV+FVSRNYYS++ KR  F 
Sbjct: 304  AETGDFLGNHRGFWFYTIGQRQGLRLPGGPWYVVEKDTKNNVVFVSRNYYSIDKKRRIFR 363

Query: 1246 VSNIRWIYATPED-FENIKCKVRHGPGFYDCKIEHAKDIYWKEHNLPHPVEDSTQEVYTV 1422
            V ++RW+   P D    ++CKVRHGPGFY C  E                 ++  +V  V
Sbjct: 364  VGSLRWLCGKPSDNVSKLRCKVRHGPGFYSCSFE----------------MEAKGDVAVV 407

Query: 1423 TLSENDQGLAAGQFAAFYKDNICLGSGIIVGSSKDAYFPVCSKALEIAKIKDKSVLGKPI 1602
             L E+DQGLAAGQFAAFY+   C+GSG+I+ S  D  FPVC+KAL++A ++DK+ LGKP+
Sbjct: 408  HLDEDDQGLAAGQFAAFYEGTTCIGSGVILESWDDQCFPVCAKALQLAAMEDKTKLGKPV 467

Query: 1603 KIGT 1614
            KI T
Sbjct: 468  KILT 471


>ref|XP_004247484.1| PREDICTED: tRNA-specific 2-thiouridylase MnmA-like [Solanum
            lycopersicum]
          Length = 489

 Score =  476 bits (1226), Expect = e-131
 Identities = 235/423 (55%), Positives = 286/423 (67%), Gaps = 1/423 (0%)
 Frame = +1

Query: 343  DEKYLQCCWPGGVKRPLKIXXXXXXXXXXXXXXXXXXXXXHHCTAFYLKIDQDYVYENYM 522
            D  YL C  P   K PLK+                     H CTAFYLKI     +ENY 
Sbjct: 66   DPTYLSCSMPH--KNPLKVAVLVSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENYW 123

Query: 523  PGCPWEDDLKYVRSVCEQAGVDLQRIDFTDRYWKSVVSYMISEYKRGFTPNPDILCNTRI 702
              CPWEDDLKY + VC+Q  V L+ +  TD YW +VVSY+I EYK G TPNPD+LCNTRI
Sbjct: 124  SECPWEDDLKYAKGVCDQVDVPLEVVHLTDEYWNNVVSYIIEEYKCGRTPNPDVLCNTRI 183

Query: 703  KFGEFLDYIDTMNFDYIASGHYARVQHSGTTSKDTSAILCLSLDNVKDQTYFXXXXXXXX 882
            KFG F+D I  M FD++ASGHYA++ H+ T   D  ++L LS D VKDQTYF        
Sbjct: 184  KFGAFMDAISGMEFDFVASGHYAKIVHASTEQLDEPSVLELSKDMVKDQTYFLSHLSQAQ 243

Query: 883  XXXXIFPLGCIPKNEVRELAADMGLPNKDRKDSQGICFLGKVKFSEFVARHIGEMEGILL 1062
                IFPLGCI K+EVR LA    LPN++RKDSQGICFLGK+KFSEFVARHIGE EGI+L
Sbjct: 244  LKRLIFPLGCIQKDEVRMLAKSFNLPNQERKDSQGICFLGKIKFSEFVARHIGESEGIIL 303

Query: 1063 EAETGAFLGNHRGFWFYTIGQRQGLGLTGGPWYVVAKDVENNVIFVSRNYYSLEMKRSFF 1242
            EAE G +LGNHRGFWFYTIGQRQGL L GGPWYVV KD++NNV++VSRNY+S++ KR  F
Sbjct: 304  EAENGDYLGNHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVYVSRNYFSVDKKRRLF 363

Query: 1243 HVSNIRWIYAT-PEDFENIKCKVRHGPGFYDCKIEHAKDIYWKEHNLPHPVEDSTQEVYT 1419
             V +++W+    P+  + ++CKVRHGPGFY+C             +L   V+   QEV  
Sbjct: 364  RVGSLKWLSGLFPKQIDELQCKVRHGPGFYNC-------------SLVMEVDQHGQEVAV 410

Query: 1420 VTLSENDQGLAAGQFAAFYKDNICLGSGIIVGSSKDAYFPVCSKALEIAKIKDKSVLGKP 1599
            V LS +DQGLAAGQFAAFY    C+GSGII+ S  D  +P+C +ALEIA+++DKS LGKP
Sbjct: 411  VRLSGDDQGLAAGQFAAFYDGRTCIGSGIILESWDDQGYPICERALEIARMEDKSKLGKP 470

Query: 1600 IKI 1608
            +KI
Sbjct: 471  VKI 473


>ref|XP_006393065.1| hypothetical protein EUTSA_v10011432mg [Eutrema salsugineum]
            gi|557089643|gb|ESQ30351.1| hypothetical protein
            EUTSA_v10011432mg [Eutrema salsugineum]
          Length = 488

 Score =  473 bits (1217), Expect = e-130
 Identities = 237/435 (54%), Positives = 291/435 (66%), Gaps = 4/435 (0%)
 Frame = +1

Query: 346  EKYLQCCWPGGVKRPLKIXXXXXXXXXXXXXXXXXXXXXHHCTAFYLKIDQDYVYENYMP 525
            ++YL C  P   ++PL++                     H CTAFYLKI     +EN+  
Sbjct: 60   DQYLSCSMP---EKPLRVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEGFENFWN 116

Query: 526  GCPWEDDLKYVRSVCEQAGVDLQRIDFTDRYWKSVVSYMISEYKRGFTPNPDILCNTRIK 705
             CPWEDDLKY + VCEQ  V L+ +  TD YW+ VVSY+I EY+ G TPNPD+LCNTRIK
Sbjct: 117  QCPWEDDLKYAKHVCEQVDVPLEVVHLTDEYWERVVSYIIEEYRCGRTPNPDVLCNTRIK 176

Query: 706  FGEFLDYIDTMNFDYIASGHYARVQHSGTTSK-DTSAILCLSLDNVKDQTYFXXXXXXXX 882
            FG F+D I  M +DYIASGHYA+V H     + D+S++L LS D VKDQTYF        
Sbjct: 177  FGAFMDTISDMEYDYIASGHYAKVVHPPPADQNDSSSLLELSQDMVKDQTYFLSHLSQTQ 236

Query: 883  XXXXIFPLGCIPKNEVRELAADMGLPNKDRKDSQGICFLGKVKFSEFVARHIGEMEGILL 1062
                +FPLGC+ K EVR+LA    LPNKDRKDSQGICFLGK+KFS+FV RHIGEMEGI+L
Sbjct: 237  LKRLLFPLGCVKKEEVRKLATKFDLPNKDRKDSQGICFLGKIKFSDFVGRHIGEMEGIIL 296

Query: 1063 EAETGAFLGNHRGFWFYTIGQRQGLGLTGGPWYVVAKDVENNVIFVSRNYYSLEMKRSFF 1242
            EAETG FLGNHRGFWFYTIGQRQGL L GGPWYVV KD +NNV+FVSRNYYS++ +R  F
Sbjct: 297  EAETGDFLGNHRGFWFYTIGQRQGLRLPGGPWYVVEKDTKNNVVFVSRNYYSIDKRRRIF 356

Query: 1243 HVSNIRWIYATPE-DFENIKCKVRHGPGFYDCKIEHAKDIYWKEHNLPHPVEDSTQEVYT 1419
             V ++RW+   P  +   ++CKVRHGPGFY C  E                 ++  +V  
Sbjct: 357  RVGSLRWLSGKPSGNVSQLRCKVRHGPGFYSCSFE----------------MEAQGDVAV 400

Query: 1420 VTLSENDQGLAAGQFAAFYKDNICLGSGIIVGSSKDAYFPVCSKALEIAKIKDKSVLGKP 1599
            V L E+DQGLAAGQFAAFY+   C+GSG+I+ S  D  FPVC+KALE+A ++DK+ LGKP
Sbjct: 401  VHLDEDDQGLAAGQFAAFYEGTTCIGSGVILESWDDQCFPVCAKALELAAMEDKTKLGKP 460

Query: 1600 IKIGT--TVKSTETK 1638
            +KI T     S ET+
Sbjct: 461  VKIMTMPVTPSVETE 475


>dbj|BAD95081.1| hypothetical protein [Arabidopsis thaliana]
          Length = 497

 Score =  473 bits (1217), Expect = e-130
 Identities = 235/432 (54%), Positives = 286/432 (66%), Gaps = 3/432 (0%)
 Frame = +1

Query: 346  EKYLQCCWPGGVKRPLKIXXXXXXXXXXXXXXXXXXXXXHHCTAFYLKIDQDYVYENYMP 525
            ++YL C  P   ++PL++                     H CTAFYLKI     +EN+  
Sbjct: 70   DQYLSCSMP---EKPLRVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEGFENFWN 126

Query: 526  GCPWEDDLKYVRSVCEQAGVDLQRIDFTDRYWKSVVSYMISEYKRGFTPNPDILCNTRIK 705
             CPWEDDLKY + VCEQ  V L+ +  TD YW+ VVSY+I EY+ G TPNPD+LCNTRIK
Sbjct: 127  QCPWEDDLKYAKHVCEQVDVPLEVVHLTDEYWERVVSYIIEEYRCGRTPNPDVLCNTRIK 186

Query: 706  FGEFLDYIDTMNFDYIASGHYARVQHSGTTSKDTSAILCLSLDNVKDQTYFXXXXXXXXX 885
            FG F+D I  M +DY+ SGHYARV H      D S++L LS D VKDQTYF         
Sbjct: 187  FGAFMDAISDMEYDYVGSGHYARVVHPPADQNDASSVLELSQDMVKDQTYFLSHLSQTQL 246

Query: 886  XXXIFPLGCIPKNEVRELAADMGLPNKDRKDSQGICFLGKVKFSEFVARHIGEMEGILLE 1065
               +FPLGC+ K+EVR+LA    LPNKDRKDSQGICFLGK+KFS+FV RHIGEMEGI+LE
Sbjct: 247  KRLLFPLGCVKKDEVRKLATQFDLPNKDRKDSQGICFLGKIKFSDFVCRHIGEMEGIILE 306

Query: 1066 AETGAFLGNHRGFWFYTIGQRQGLGLTGGPWYVVAKDVENNVIFVSRNYYSLEMKRSFFH 1245
            AE+G FLGNHRGFWFYTIGQRQGL L GGPWYVV KD +NNV+FVSRNYYS++ +R  F 
Sbjct: 307  AESGDFLGNHRGFWFYTIGQRQGLRLPGGPWYVVEKDTKNNVVFVSRNYYSIDKRRRIFR 366

Query: 1246 VSNIRWIYATPE-DFENIKCKVRHGPGFYDCKIEHAKDIYWKEHNLPHPVEDSTQEVYTV 1422
            V ++RW    P      ++CKVRHGPGFY C  E   D                 +V  V
Sbjct: 367  VGSLRWNSGKPSGKVRELRCKVRHGPGFYSCSFEMEGD----------------GDVAVV 410

Query: 1423 TLSENDQGLAAGQFAAFYKDNICLGSGIIVGSSKDAYFPVCSKALEIAKIKDKSVLGKPI 1602
             L E+DQGLAAGQFAAFY+   C+GSG+I+ S  D  FPVC+KAL++A ++DK+ LGKP+
Sbjct: 411  HLDEDDQGLAAGQFAAFYEGTTCIGSGVILESWDDQCFPVCAKALQLAALEDKTKLGKPV 470

Query: 1603 KIGT--TVKSTE 1632
            KI T  T+ S E
Sbjct: 471  KIMTMPTMSSVE 482


>ref|XP_004507093.1| PREDICTED: tRNA-specific 2-thiouridylase MnmA-like [Cicer arietinum]
          Length = 484

 Score =  472 bits (1215), Expect = e-130
 Identities = 234/422 (55%), Positives = 283/422 (67%), Gaps = 1/422 (0%)
 Frame = +1

Query: 346  EKYLQCCWPGGVKRPLKIXXXXXXXXXXXXXXXXXXXXXHHCTAFYLKIDQDYVYENYMP 525
            + YL+C  P     PL++                     H CTAFYLKI     +EN+  
Sbjct: 57   DPYLRCSMP---HNPLRVAVLISGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWS 113

Query: 526  GCPWEDDLKYVRSVCEQAGVDLQRIDFTDRYWKSVVSYMISEYKRGFTPNPDILCNTRIK 705
             CPWEDDLKY + VC Q  V L+ +  TD YWK+VVSY+I EY+ G TPNPD+LCNTRIK
Sbjct: 114  ECPWEDDLKYAKDVCNQVEVPLEVVHLTDEYWKNVVSYIIEEYQCGRTPNPDVLCNTRIK 173

Query: 706  FGEFLDYIDTMNFDYIASGHYARVQHSGTTSKDTSAILCLSLDNVKDQTYFXXXXXXXXX 885
            FG FLD I  M FDY+ASGHYA V H      D  + L LS D VKDQTYF         
Sbjct: 174  FGAFLDAISDMGFDYVASGHYANVIHPSADQMDGPSTLELSQDMVKDQTYFLSHLSQSQL 233

Query: 886  XXXIFPLGCIPKNEVRELAADMGLPNKDRKDSQGICFLGKVKFSEFVARHIGEMEGILLE 1065
               +FPLGCIPK+EVR LA    LPNKDRKDSQGICFLGK+KFSEFVARHIGE EGI+LE
Sbjct: 234  KQLLFPLGCIPKDEVRRLATKFDLPNKDRKDSQGICFLGKIKFSEFVARHIGEKEGIILE 293

Query: 1066 AETGAFLGNHRGFWFYTIGQRQGLGLTGGPWYVVAKDVENNVIFVSRNYYSLEMKRSFFH 1245
            AETG FLG HRGFWFYTIGQRQGL L GGPWYVV KDV+NNV+FVSRNY+S++ +R  F 
Sbjct: 294  AETGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSIDKRRRVFR 353

Query: 1246 VSNIRWIYA-TPEDFENIKCKVRHGPGFYDCKIEHAKDIYWKEHNLPHPVEDSTQEVYTV 1422
            V +++W+    P     ++CKVRHGPGFYDC ++        E    +P++ +      V
Sbjct: 354  VGSLKWLSGLPPSQTSQLRCKVRHGPGFYDCSLQ-------MELEGENPIDSA-----VV 401

Query: 1423 TLSENDQGLAAGQFAAFYKDNICLGSGIIVGSSKDAYFPVCSKALEIAKIKDKSVLGKPI 1602
             +SE+DQGLAAGQF AFY+   C+GSG+I+ S  D  FPVC+KALEIA+++DKS +G P+
Sbjct: 402  RISEDDQGLAAGQFTAFYEGRTCIGSGVILESWDDQGFPVCTKALEIARMEDKSKIGNPV 461

Query: 1603 KI 1608
            KI
Sbjct: 462  KI 463


>ref|XP_004301461.1| PREDICTED: tRNA-specific 2-thiouridylase MnmA-like [Fragaria vesca
            subsp. vesca]
          Length = 475

 Score =  472 bits (1214), Expect = e-130
 Identities = 231/422 (54%), Positives = 289/422 (68%), Gaps = 3/422 (0%)
 Frame = +1

Query: 352  YLQCCWPGGVKRPLKIXXXXXXXXXXXXXXXXXXXXXHHCTAFYLKIDQDYVYENYMPGC 531
            YL C  P   K+PLK+                     H CTAFYLKI     +EN+   C
Sbjct: 63   YLSCSMPH--KKPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEGFENFWSEC 120

Query: 532  PWEDDLKYVRSVCEQAGVDLQRIDFTDRYWKSVVSYMISEYKRGFTPNPDILCNTRIKFG 711
            PWE+DL Y ++VC Q  V L+ +  TD YWK+VVSY+I EY+ G TPNPD+LCNTRIKFG
Sbjct: 121  PWEEDLNYAKAVCNQVDVPLEVVHLTDEYWKNVVSYIIEEYRCGRTPNPDVLCNTRIKFG 180

Query: 712  EFLDYIDTMNFDYIASGHYARVQHSGTTSKDTSAILCLSLDNVKDQTYFXXXXXXXXXXX 891
             F+D ID+M FDY+ASGHYA+V H      D  ++L LS D VKDQTYF           
Sbjct: 181  VFMDAIDSMEFDYVASGHYAKVVHLSADQTDKDSVLELSQDMVKDQTYFLSHLSQDQLKK 240

Query: 892  XIFPLGCIPKNEVRELAADMGLPNKDRKDSQGICFLGKVKFSEFVARHIGEMEGILLEAE 1071
             IFPLGC+ K++VR+LA    LPNK+RKDSQGICFLGKV+FSEF+ +HIGE EG++LEAE
Sbjct: 241  LIFPLGCLSKDKVRKLATKFDLPNKERKDSQGICFLGKVRFSEFIGKHIGEKEGVILEAE 300

Query: 1072 TGAFLGNHRGFWFYTIGQRQGLGLTGGPWYVVAKDVENNVIFVSRNYYSLEMKRSFFHVS 1251
            TG  LG HRGFWFYTIGQRQGL L GGPWYVV KD+++NV+FVSRNYYS++ +R  F V 
Sbjct: 301  TGDLLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKHNVVFVSRNYYSVDKRRRLFRVG 360

Query: 1252 NIRWIYAT-PEDFENIKCKVRHGPGFYDC--KIEHAKDIYWKEHNLPHPVEDSTQEVYTV 1422
            +++W++ + P     ++CKVRHGP F +C  KIEH               ED  ++V  V
Sbjct: 361  SLKWLHGSPPAQISQLQCKVRHGPRFCNCTVKIEHG--------------EDGNEDVAVV 406

Query: 1423 TLSENDQGLAAGQFAAFYKDNICLGSGIIVGSSKDAYFPVCSKALEIAKIKDKSVLGKPI 1602
            +LSE+DQGLAAGQFAAFY+   CLGSG+I+ S  D  FPVC+KALE A+++DKS+LGKP+
Sbjct: 407  SLSEDDQGLAAGQFAAFYQGRTCLGSGVILESWDDQGFPVCTKALENARMEDKSLLGKPV 466

Query: 1603 KI 1608
            KI
Sbjct: 467  KI 468


>ref|XP_002894309.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase
            [Arabidopsis lyrata subsp. lyrata]
            gi|297340151|gb|EFH70568.1| tRNA
            (5-methylaminomethyl-2-thiouridylate)-methyltransferase
            [Arabidopsis lyrata subsp. lyrata]
          Length = 500

 Score =  472 bits (1214), Expect = e-130
 Identities = 231/424 (54%), Positives = 282/424 (66%), Gaps = 1/424 (0%)
 Frame = +1

Query: 346  EKYLQCCWPGGVKRPLKIXXXXXXXXXXXXXXXXXXXXXHHCTAFYLKIDQDYVYENYMP 525
            ++YL C  P   ++PL++                     H CTAFYLKI     +EN+  
Sbjct: 73   DQYLSCSMP---EKPLRVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEGFENFWN 129

Query: 526  GCPWEDDLKYVRSVCEQAGVDLQRIDFTDRYWKSVVSYMISEYKRGFTPNPDILCNTRIK 705
             CPWEDDLKY + VCEQ  V L+ +  TD YW+ VVSY+I EY+ G TPNPD+LCNTRIK
Sbjct: 130  QCPWEDDLKYAKHVCEQVDVPLEVVHLTDEYWERVVSYIIEEYRCGRTPNPDVLCNTRIK 189

Query: 706  FGEFLDYIDTMNFDYIASGHYARVQHSGTTSKDTSAILCLSLDNVKDQTYFXXXXXXXXX 885
            FG F+D I  M +DY+ SGHYA+V H      D S++L LS D VKDQTYF         
Sbjct: 190  FGAFMDAISDMEYDYVGSGHYAKVVHPPVDQNDASSVLELSQDMVKDQTYFLSHLSQTQL 249

Query: 886  XXXIFPLGCIPKNEVRELAADMGLPNKDRKDSQGICFLGKVKFSEFVARHIGEMEGILLE 1065
               IFPLGC+ K+EVR+LA    LPNKDRKDSQGICFLGK+KFS+FV  HIGEMEGI+LE
Sbjct: 250  KRLIFPLGCVKKDEVRKLATQFDLPNKDRKDSQGICFLGKIKFSDFVCAHIGEMEGIILE 309

Query: 1066 AETGAFLGNHRGFWFYTIGQRQGLGLTGGPWYVVAKDVENNVIFVSRNYYSLEMKRSFFH 1245
            AETG FLGNHRGFWFYTIGQRQGL L GGPWYVV KD +NNV+FVSRNYYS++ +R  F 
Sbjct: 310  AETGDFLGNHRGFWFYTIGQRQGLRLPGGPWYVVEKDTKNNVVFVSRNYYSIDKRRRIFR 369

Query: 1246 VSNIRWIYATPE-DFENIKCKVRHGPGFYDCKIEHAKDIYWKEHNLPHPVEDSTQEVYTV 1422
            V ++RW+   P  +   ++CKVRHGP FY C  E   D                 +V  V
Sbjct: 370  VGSLRWLSGKPSGNVRELRCKVRHGPSFYSCSFEMEAD----------------GDVAVV 413

Query: 1423 TLSENDQGLAAGQFAAFYKDNICLGSGIIVGSSKDAYFPVCSKALEIAKIKDKSVLGKPI 1602
             L E+DQGLAAGQFAAFY+   C+GSG+I+ S  D  FPVC+KAL++A ++DK+ LGKP+
Sbjct: 414  HLDEDDQGLAAGQFAAFYEGTTCIGSGVILESWDDQCFPVCAKALQLAAMEDKTKLGKPV 473

Query: 1603 KIGT 1614
            KI T
Sbjct: 474  KIRT 477


>ref|NP_175542.2| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase
            [Arabidopsis thaliana] gi|332194528|gb|AEE32649.1| tRNA
            (5-methylaminomethyl-2-thiouridylate)-methyltransferase
            [Arabidopsis thaliana]
          Length = 497

 Score =  472 bits (1214), Expect = e-130
 Identities = 234/432 (54%), Positives = 286/432 (66%), Gaps = 3/432 (0%)
 Frame = +1

Query: 346  EKYLQCCWPGGVKRPLKIXXXXXXXXXXXXXXXXXXXXXHHCTAFYLKIDQDYVYENYMP 525
            ++YL C  P   ++PL++                     H CTAFYLKI     +EN+  
Sbjct: 70   DQYLSCSMP---EKPLRVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEGFENFWN 126

Query: 526  GCPWEDDLKYVRSVCEQAGVDLQRIDFTDRYWKSVVSYMISEYKRGFTPNPDILCNTRIK 705
             CPWEDDLKY + VCEQ  V L+ +  TD YW+ VVSY+I EY+ G TPNPD+LCNTRIK
Sbjct: 127  QCPWEDDLKYAKHVCEQVDVPLEVVHLTDEYWERVVSYIIEEYRCGRTPNPDVLCNTRIK 186

Query: 706  FGEFLDYIDTMNFDYIASGHYARVQHSGTTSKDTSAILCLSLDNVKDQTYFXXXXXXXXX 885
            FG F+D I  M +DY+ SGHYA+V H      D S++L LS D VKDQTYF         
Sbjct: 187  FGAFMDAISDMEYDYVGSGHYAKVVHPPADQNDASSVLELSQDMVKDQTYFLSHLSQTQL 246

Query: 886  XXXIFPLGCIPKNEVRELAADMGLPNKDRKDSQGICFLGKVKFSEFVARHIGEMEGILLE 1065
               +FPLGC+ K+EVR+LA    LPNKDRKDSQGICFLGK+KFS+FV RHIGEMEGI+LE
Sbjct: 247  KRLLFPLGCVKKDEVRKLATQFDLPNKDRKDSQGICFLGKIKFSDFVCRHIGEMEGIILE 306

Query: 1066 AETGAFLGNHRGFWFYTIGQRQGLGLTGGPWYVVAKDVENNVIFVSRNYYSLEMKRSFFH 1245
            AE+G FLGNHRGFWFYTIGQRQGL L GGPWYVV KD +NNV+FVSRNYYS++ +R  F 
Sbjct: 307  AESGDFLGNHRGFWFYTIGQRQGLRLPGGPWYVVEKDTKNNVVFVSRNYYSIDKRRRIFR 366

Query: 1246 VSNIRWIYATPE-DFENIKCKVRHGPGFYDCKIEHAKDIYWKEHNLPHPVEDSTQEVYTV 1422
            V ++RW    P      ++CKVRHGPGFY C  E   D                 +V  V
Sbjct: 367  VGSLRWNSGKPSGKVRELRCKVRHGPGFYSCSFEMEGD----------------GDVAVV 410

Query: 1423 TLSENDQGLAAGQFAAFYKDNICLGSGIIVGSSKDAYFPVCSKALEIAKIKDKSVLGKPI 1602
             L E+DQGLAAGQFAAFY+   C+GSG+I+ S  D  FPVC+KAL++A ++DK+ LGKP+
Sbjct: 411  HLDEDDQGLAAGQFAAFYEGTTCIGSGVILESWDDQCFPVCAKALQLAALEDKTKLGKPV 470

Query: 1603 KIGT--TVKSTE 1632
            KI T  T+ S E
Sbjct: 471  KIMTMPTMSSVE 482


>ref|XP_003539065.2| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1-like isoform
            X1 [Glycine max]
          Length = 502

 Score =  468 bits (1204), Expect = e-129
 Identities = 230/422 (54%), Positives = 282/422 (66%), Gaps = 1/422 (0%)
 Frame = +1

Query: 346  EKYLQCCWPGGVKRPLKIXXXXXXXXXXXXXXXXXXXXXHHCTAFYLKIDQDYVYENYMP 525
            ++YL+C  P     PL++                     H CTAFYLKI     +EN+  
Sbjct: 63   DRYLRCSMPQN--SPLRVAVLVSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWS 120

Query: 526  GCPWEDDLKYVRSVCEQAGVDLQRIDFTDRYWKSVVSYMISEYKRGFTPNPDILCNTRIK 705
             CPWEDDLKY + VC Q  V L+ +  TD YW +VVSY+I EY  G TPNPD+LCNTRIK
Sbjct: 121  ECPWEDDLKYAKDVCNQVDVPLEVVHLTDEYWNNVVSYLIEEYSSGRTPNPDVLCNTRIK 180

Query: 706  FGEFLDYIDTMNFDYIASGHYARVQHSGTTSKDTSAILCLSLDNVKDQTYFXXXXXXXXX 885
            FG FLD I  M FDY+ASGHYA V H  +  +D  ++L LS D VKDQTYF         
Sbjct: 181  FGAFLDAIGGMGFDYVASGHYANVIHPCSDRRDEPSVLELSQDMVKDQTYFLSHLSQSQL 240

Query: 886  XXXIFPLGCIPKNEVRELAADMGLPNKDRKDSQGICFLGKVKFSEFVARHIGEMEGILLE 1065
               +FPLGCIPK+EVR+LA    LPNKDRKDSQGICFLGK++FSEFVARHIGE EG++LE
Sbjct: 241  KQLLFPLGCIPKDEVRKLATKFDLPNKDRKDSQGICFLGKIRFSEFVARHIGEREGVILE 300

Query: 1066 AETGAFLGNHRGFWFYTIGQRQGLGLTGGPWYVVAKDVENNVIFVSRNYYSLEMKRSFFH 1245
            AETG FLG HRGFWFYTIGQRQGL L GGPWYVV KDV+NNV+FVSRNY+S + +R  F 
Sbjct: 301  AETGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSFDKRRRVFR 360

Query: 1246 VSNIRWIYATPE-DFENIKCKVRHGPGFYDCKIEHAKDIYWKEHNLPHPVEDSTQEVYTV 1422
            V + +W+   P      ++CKVRHGPGFYDC ++   +   + H+              V
Sbjct: 361  VGSFKWLSGLPPGQTTQLQCKVRHGPGFYDCSLQMEVEADGQCHSA------------VV 408

Query: 1423 TLSENDQGLAAGQFAAFYKDNICLGSGIIVGSSKDAYFPVCSKALEIAKIKDKSVLGKPI 1602
             +SE+DQGLAAGQFAAFY+   C+GSG+I+    D  FPVC+KALEIA+++DKS +G P+
Sbjct: 409  RISEDDQGLAAGQFAAFYEGRTCIGSGVILEFWDDQSFPVCTKALEIARMEDKSKIGNPV 468

Query: 1603 KI 1608
            KI
Sbjct: 469  KI 470


>ref|XP_002468413.1| hypothetical protein SORBIDRAFT_01g045520 [Sorghum bicolor]
            gi|241922267|gb|EER95411.1| hypothetical protein
            SORBIDRAFT_01g045520 [Sorghum bicolor]
          Length = 489

 Score =  468 bits (1204), Expect = e-129
 Identities = 234/422 (55%), Positives = 290/422 (68%), Gaps = 1/422 (0%)
 Frame = +1

Query: 346  EKYLQCCWPGGVKRPLKIXXXXXXXXXXXXXXXXXXXXXHHCTAFYLKIDQDYVYENYMP 525
            E++L  C   G + PL++                     HHCTAFYLKI     + N+  
Sbjct: 64   EEHLTRCAAAG-RAPLRVAVLVSGGVDSSVALRLLHAAGHHCTAFYLKIWFQEDFRNFWS 122

Query: 526  GCPWEDDLKYVRSVCEQAGVDLQRIDFTDRYWKSVVSYMISEYKRGFTPNPDILCNTRIK 705
             CPW++DLKY ++VC++  V L+ +  +D YW  VVS+MI+EY+ G TPNPD+LCNTRIK
Sbjct: 123  ECPWDEDLKYAQAVCDKIDVPLEVVHLSDEYWNYVVSHMINEYRCGRTPNPDVLCNTRIK 182

Query: 706  FGEFLDYIDTMNFDYIASGHYARVQHSGTTSKDTSAILCLSLDNVKDQTYFXXXXXXXXX 885
            FG FL+ I+ + FDYIASGHYA V H  T + +  +IL LS D VKDQTYF         
Sbjct: 183  FGAFLEAIENLGFDYIASGHYAHVVHPSTENTEAPSILQLSKDKVKDQTYFLSHLSQPQL 242

Query: 886  XXXIFPLGCIPKNEVRELAADMGLPNKDRKDSQGICFLGKVKFSEFVARHIGEMEGILLE 1065
               +FPLGCI K+EVR LA  MGLPN+ RKDSQGICFLGKVKFSEFV RHIGEMEGI+LE
Sbjct: 243  RRLLFPLGCITKDEVRRLATQMGLPNQGRKDSQGICFLGKVKFSEFVERHIGEMEGIILE 302

Query: 1066 AETGAFLGNHRGFWFYTIGQRQGLGLTGGPWYVVAKDVENNVIFVSRNYYSLEMKRSFFH 1245
            AE+G +LG HRGFWFYTIGQRQGL L GGPWYVV KDV+NNV+FVSRNYYSL+ +R  F 
Sbjct: 303  AESGDYLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVQNNVVFVSRNYYSLDKRRRTFR 362

Query: 1246 VSNIRWIYAT-PEDFENIKCKVRHGPGFYDCKIEHAKDIYWKEHNLPHPVEDSTQEVYTV 1422
            V ++ W  ++ P + E +KCKVRH P F+DC +        KEH       +  ++   V
Sbjct: 363  VGSLNWFDSSGPGNNEQLKCKVRHSPEFHDCTL-------IKEHT------EENEDALVV 409

Query: 1423 TLSENDQGLAAGQFAAFYKDNICLGSGIIVGSSKDAYFPVCSKALEIAKIKDKSVLGKPI 1602
             LSE+DQGLAAGQFAAFY ++ CLGSGII+ S     FPVCS+ALEIAK++DKS LGKP+
Sbjct: 410  HLSEDDQGLAAGQFAAFYSEHSCLGSGIILDSWDKMSFPVCSRALEIAKLEDKSSLGKPV 469

Query: 1603 KI 1608
            +I
Sbjct: 470  RI 471