BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dioscorea21_contig00035678
         (641 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002305237.1| acyl:coa ligase acetate-coa synthetase-like ...   317   1e-84
ref|XP_002272437.1| PREDICTED: medium-chain-fatty-acid--CoA liga...   315   3e-84
ref|XP_002327560.1| predicted protein [Populus trichocarpa] gi|2...   310   2e-82
ref|XP_002301652.1| acyl:coa ligase acetate-coa synthetase-like ...   307   1e-81
gb|AFS60176.1| cinnamate:CoA ligase [Hypericum calycinum]             305   4e-81

>ref|XP_002305237.1| acyl:coa ligase acetate-coa synthetase-like protein [Populus
           trichocarpa] gi|118487892|gb|ABK95768.1| unknown
           [Populus trichocarpa] gi|222848201|gb|EEE85748.1|
           acyl:coa ligase acetate-coa synthetase-like protein
           [Populus trichocarpa]
          Length = 584

 Score =  317 bits (812), Expect = 1e-84
 Identities = 149/214 (69%), Positives = 176/214 (82%), Gaps = 4/214 (1%)
 Frame = +1

Query: 10  IDDIDTPTGVVRHGKFEYEELIARGDPMHAPHVLEDEWDAIALNYTSGTTSAPKGVVYSH 189
           IDD+DTPTGV R G+FEYE+L+ RG+P +   +++DEWD IALNYTSGTTSAPKGVVYSH
Sbjct: 143 IDDVDTPTGV-RFGQFEYEQLVQRGNPGYTGELIQDEWDPIALNYTSGTTSAPKGVVYSH 201

Query: 190 RGAYISCMTLLLQWGVGNEPVYLWSLPMFHCNGWTFTWGMAARGGTNVCIRNTSAAEMHR 369
           RGAY+S ++L+L W +G+ PVYLWSLPMFHCNGWTFTWG+AARGGTNVCIRNTSA +M++
Sbjct: 202 RGAYLSTLSLILGWEMGSAPVYLWSLPMFHCNGWTFTWGVAARGGTNVCIRNTSAKDMYQ 261

Query: 370 AITQYKVTHMCCAPIVLKIIFEGFDCNMSATATLHRR----PVVEILTGGAPPPAALLEK 537
           +I  +KVTHMCCAPIV  I+ E         A  H R      VEILTGGAPPPA+LLEK
Sbjct: 262 SIADHKVTHMCCAPIVFNILLE---------AKPHERREITSPVEILTGGAPPPASLLEK 312

Query: 538 IENLGFHVTHAYGLTEATGPATVCEWKAEWNELP 639
           IE+LGFHVTHAYGLTEATGPA VCEW+ +WN+LP
Sbjct: 313 IESLGFHVTHAYGLTEATGPALVCEWQTKWNKLP 346


>ref|XP_002272437.1| PREDICTED: medium-chain-fatty-acid--CoA ligase [Vitis vinifera]
          Length = 588

 Score =  315 bits (808), Expect = 3e-84
 Identities = 150/213 (70%), Positives = 173/213 (81%)
 Frame = +1

Query: 1   LILIDDIDTPTGVVRHGKFEYEELIARGDPMHAPHVLEDEWDAIALNYTSGTTSAPKGVV 180
           +I+IDDIDTPTGV R G+ EYE+L+A G+P +AP  +EDEWD IALNYTSGTTSAPKGVV
Sbjct: 149 VIVIDDIDTPTGV-RLGELEYEQLVANGNPGYAPVEVEDEWDPIALNYTSGTTSAPKGVV 207

Query: 181 YSHRGAYISCMTLLLQWGVGNEPVYLWSLPMFHCNGWTFTWGMAARGGTNVCIRNTSAAE 360
           YSHRGAY+S ++LLL W +G EPVY+WSLPMFHCNGWTFTWG+AARGGT+VCIRNT+A +
Sbjct: 208 YSHRGAYLSTLSLLLGWEMGGEPVYMWSLPMFHCNGWTFTWGVAARGGTSVCIRNTTAYD 267

Query: 361 MHRAITQYKVTHMCCAPIVLKIIFEGFDCNMSATATLHRRPVVEILTGGAPPPAALLEKI 540
           M+R I  + VTHMCCAPIV  I+ E  +       T      V+ILTGGAPPPA LLEKI
Sbjct: 268 MYRNIALHNVTHMCCAPIVFNILLEAKENERQRLKT-----PVQILTGGAPPPAPLLEKI 322

Query: 541 ENLGFHVTHAYGLTEATGPATVCEWKAEWNELP 639
           E LGFHV HAYGLTEATGPA VCEW+AEWN LP
Sbjct: 323 EALGFHVVHAYGLTEATGPALVCEWQAEWNRLP 355


>ref|XP_002327560.1| predicted protein [Populus trichocarpa] gi|222836114|gb|EEE74535.1|
           predicted protein [Populus trichocarpa]
          Length = 547

 Score =  310 bits (793), Expect = 2e-82
 Identities = 146/214 (68%), Positives = 172/214 (80%), Gaps = 4/214 (1%)
 Frame = +1

Query: 10  IDDIDTPTGVVRHGKFEYEELIARGDPMHAPHVLEDEWDAIALNYTSGTTSAPKGVVYSH 189
           IDDIDTPTGV + G+ EYE+L+ RG+P +   +++DEWD IALNYTSGTTSAPKGVVYSH
Sbjct: 143 IDDIDTPTGV-QFGQLEYEQLVQRGNPGYTDELVQDEWDPIALNYTSGTTSAPKGVVYSH 201

Query: 190 RGAYISCMTLLLQWGVGNEPVYLWSLPMFHCNGWTFTWGMAARGGTNVCIRNTSAAEMHR 369
           RGAY+S ++L+L W +GN PVYLWSLPMFHCNGWTFTWG+AARGGTNVC+RNTSA +M+ 
Sbjct: 202 RGAYLSSLSLILGWEMGNAPVYLWSLPMFHCNGWTFTWGVAARGGTNVCLRNTSAKDMYN 261

Query: 370 AITQYKVTHMCCAPIVLKIIFEGFDCNMSATATLHRR----PVVEILTGGAPPPAALLEK 537
            I ++ VTHMCCAPIV  I+ E         A  H R      VEILTGGAPPPA+LL+K
Sbjct: 262 NIAEHAVTHMCCAPIVFNILLE---------ARPHERREITSPVEILTGGAPPPASLLQK 312

Query: 538 IENLGFHVTHAYGLTEATGPATVCEWKAEWNELP 639
           IE LGFHVTHAYGLTEATGPA VCEW+ +WN+LP
Sbjct: 313 IERLGFHVTHAYGLTEATGPALVCEWQKKWNKLP 346


>ref|XP_002301652.1| acyl:coa ligase acetate-coa synthetase-like protein [Populus
           trichocarpa] gi|222843378|gb|EEE80925.1| acyl:coa ligase
           acetate-coa synthetase-like protein [Populus
           trichocarpa]
          Length = 586

 Score =  307 bits (787), Expect = 1e-81
 Identities = 145/214 (67%), Positives = 171/214 (79%), Gaps = 4/214 (1%)
 Frame = +1

Query: 10  IDDIDTPTGVVRHGKFEYEELIARGDPMHAPHVLEDEWDAIALNYTSGTTSAPKGVVYSH 189
           IDDIDTPTGV + G+ EYE+L+ RG+P +   +++DEWD IALNYTSGTTSAPKGVVYSH
Sbjct: 143 IDDIDTPTGV-QFGQLEYEQLVQRGNPGYTGELVQDEWDPIALNYTSGTTSAPKGVVYSH 201

Query: 190 RGAYISCMTLLLQWGVGNEPVYLWSLPMFHCNGWTFTWGMAARGGTNVCIRNTSAAEMHR 369
           RGAY+S ++L+L W +GN PVYLWSLPMFHCNGWTFTWG+AARGGTNVCIRNTSA +M+ 
Sbjct: 202 RGAYLSSLSLILGWEMGNAPVYLWSLPMFHCNGWTFTWGVAARGGTNVCIRNTSAKDMYH 261

Query: 370 AITQYKVTHMCCAPIVLKIIFEGFDCNMSATATLHRR----PVVEILTGGAPPPAALLEK 537
            I ++ VTHMCCAPIV  ++ E         A  H R      VEILTGGAPPPA+LL+ 
Sbjct: 262 NIAEHAVTHMCCAPIVFNVLLE---------ARPHERREITSPVEILTGGAPPPASLLQD 312

Query: 538 IENLGFHVTHAYGLTEATGPATVCEWKAEWNELP 639
           IE LGFHVTHAYGLTEATGPA VCEW+ +WN+LP
Sbjct: 313 IERLGFHVTHAYGLTEATGPALVCEWQKKWNKLP 346


>gb|AFS60176.1| cinnamate:CoA ligase [Hypericum calycinum]
          Length = 572

 Score =  305 bits (782), Expect = 4e-81
 Identities = 145/216 (67%), Positives = 171/216 (79%), Gaps = 3/216 (1%)
 Frame = +1

Query: 1   LILIDDIDTPTGVVRHGKFEYEELIARGDPMHAPHVLEDEWDAIALNYTSGTTSAPKGVV 180
           ++LIDD+D PTG+ RHG  EYE+LI RG    AP  L DEWD IALNYTSGTTSAPKGVV
Sbjct: 138 VVLIDDVDKPTGIGRHG-LEYEQLIKRGRSDFAPDELVDEWDPIALNYTSGTTSAPKGVV 196

Query: 181 YSHRGAYISCMTLLLQWGVGNEPVYLWSLPMFHCNGWTFTWGMAARGGTNVCIRNTSAAE 360
           YSHRGAY+S ++L+L W +GNEPVYLWSLPMFHCNGWTFTWG+AARGGTNVCIRNT+A +
Sbjct: 197 YSHRGAYLSTLSLILGWEMGNEPVYLWSLPMFHCNGWTFTWGIAARGGTNVCIRNTTAED 256

Query: 361 MHRAITQYKVTHMCCAPIVLKIIFEGFDCNMSATATLHRRPV---VEILTGGAPPPAALL 531
           M+R I  + VTHMCCAPIV  I+ E          +  RRP+   V++LTGGAPPP +LL
Sbjct: 257 MYRNIANHGVTHMCCAPIVFNILLE--------AGSEARRPITRPVQVLTGGAPPPESLL 308

Query: 532 EKIENLGFHVTHAYGLTEATGPATVCEWKAEWNELP 639
            K+E LGF +THAYGLTEATGPA VCEW+A+W+ LP
Sbjct: 309 AKMEPLGFKITHAYGLTEATGPALVCEWQAQWDGLP 344