BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dioscorea21_contig00030276
         (466 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002319761.1| flavonoid 3'-hydroxylase [Populus trichocarp...   133   2e-29
dbj|BAH89259.1| putative flavonoid 3'-hydroxylase [Diospyros kaki]    132   4e-29
dbj|BAF49324.1| flavonoid 3'-hydroxylase [Lobelia erinus]             131   5e-29
dbj|BAM28972.1| flavonoid 3'-hydroxylase [Lilium hybrid division I]   130   9e-29
ref|NP_001064338.1| Os10g0320100 [Oryza sativa Japonica Group] g...   130   1e-28

>ref|XP_002319761.1| flavonoid 3'-hydroxylase [Populus trichocarpa]
           gi|222858137|gb|EEE95684.1| flavonoid 3'-hydroxylase
           [Populus trichocarpa]
          Length = 521

 Score =  133 bits (334), Expect = 2e-29
 Identities = 73/155 (47%), Positives = 101/155 (65%), Gaps = 3/155 (1%)
 Frame = +1

Query: 10  RRVFEEDNG---PDVEEFMKVLEENLKLATSMNIEDFIPWVKAWDVRGFVAKLKRLHRWY 180
           RRVF + +G   P  +EF  ++ E + LA   NI DF+P ++  D++G  AK+K+LH+ +
Sbjct: 189 RRVFGDGSGDGDPKADEFKSMVVEVMVLAGVFNIGDFVPALEWLDLQGVAAKMKKLHKRF 248

Query: 181 DGTLTKIIEEHKTKPKIVSEGEAEGKDLLSVLLRLKEADVKDKETDNYKLTDTDIKALLT 360
           D  LT I+EEHKT     S    +  DLLS L+ LKE   +D + +  KLTDT+IKALL 
Sbjct: 249 DAFLTNIVEEHKTSSSTASVRSEKHTDLLSTLIALKEQ--QDVDGEEGKLTDTEIKALLL 306

Query: 361 DLFIGGTDTTSLMVEWALVELIRHPHILAAAQKEL 465
           ++F  GTDT+S  VEWA+ ELIRHP ILA  ++EL
Sbjct: 307 NMFTAGTDTSSSTVEWAIAELIRHPDILAQVKQEL 341


>dbj|BAH89259.1| putative flavonoid 3'-hydroxylase [Diospyros kaki]
          Length = 416

 Score =  132 bits (331), Expect = 4e-29
 Identities = 76/153 (49%), Positives = 103/153 (67%), Gaps = 1/153 (0%)
 Frame = +1

Query: 10  RRVF-EEDNGPDVEEFMKVLEENLKLATSMNIEDFIPWVKAWDVRGFVAKLKRLHRWYDG 186
           RRVF ++D+G  V+EF +++ E + LA   N+ DF+P ++  D++G  AK+K+LHR +D 
Sbjct: 92  RRVFRDDDSGGGVDEFKQMVVELMVLAGVFNLGDFVPALEWLDLQGVAAKMKKLHRRFDV 151

Query: 187 TLTKIIEEHKTKPKIVSEGEAEGKDLLSVLLRLKEADVKDKETDNYKLTDTDIKALLTDL 366
            LT I+EEHK  P     G  +  DLLS L+ LK+AD   +     +LTDT+IKALL DL
Sbjct: 152 FLTGILEEHKRHPG----GGQKHTDLLSTLISLKDADTDGE--GGGRLTDTEIKALLLDL 205

Query: 367 FIGGTDTTSLMVEWALVELIRHPHILAAAQKEL 465
           F  GTDT++  VEWA+ ELIRHP ILA AQ EL
Sbjct: 206 FTAGTDTSASTVEWAMAELIRHPKILARAQIEL 238


>dbj|BAF49324.1| flavonoid 3'-hydroxylase [Lobelia erinus]
          Length = 515

 Score =  131 bits (330), Expect = 5e-29
 Identities = 75/155 (48%), Positives = 102/155 (65%)
 Frame = +1

Query: 1   LFRRRVFEEDNGPDVEEFMKVLEENLKLATSMNIEDFIPWVKAWDVRGFVAKLKRLHRWY 180
           +F +RVF        +EF +++ E + LA   NI DFIP ++ +D++G V K+K+LH  +
Sbjct: 189 MFGKRVFGGGGDSKADEFKQMVVEAMVLAGVFNIGDFIPALEWFDLQGVVGKMKKLHVRF 248

Query: 181 DGTLTKIIEEHKTKPKIVSEGEAEGKDLLSVLLRLKEADVKDKETDNYKLTDTDIKALLT 360
           D  LT I+EEHKT     S G  +  DLLS L+ LK+ D+     +  KL+DT+IKALL 
Sbjct: 249 DSFLTAILEEHKTTG---SRGGGKHVDLLSTLISLKDNDLAG---EGEKLSDTEIKALLL 302

Query: 361 DLFIGGTDTTSLMVEWALVELIRHPHILAAAQKEL 465
           ++FI GTDT+S  VEWAL ELIRHP ILA AQ+EL
Sbjct: 303 NMFIAGTDTSSSTVEWALAELIRHPKILAQAQQEL 337


>dbj|BAM28972.1| flavonoid 3'-hydroxylase [Lilium hybrid division I]
          Length = 514

 Score =  130 bits (328), Expect = 9e-29
 Identities = 76/146 (52%), Positives = 98/146 (67%), Gaps = 2/146 (1%)
 Frame = +1

Query: 34  GPDV--EEFMKVLEENLKLATSMNIEDFIPWVKAWDVRGFVAKLKRLHRWYDGTLTKIIE 207
           G DV  EEF  ++ E ++LA   NI DF+P +   D++G V K+K+LH+  D  L K+I 
Sbjct: 194 GEDVTAEEFKDMVMEVMRLAGVFNIGDFVPGLGWLDLQGVVGKMKKLHKRMDSFLDKLIA 253

Query: 208 EHKTKPKIVSEGEAEGKDLLSVLLRLKEADVKDKETDNYKLTDTDIKALLTDLFIGGTDT 387
           EH+     V+ GE  GKDLLSV++RL+E    + E +  KLTDT+IKALL +LF  GTDT
Sbjct: 254 EHRAA---VAGGELAGKDLLSVMVRLQE----EGEGEGGKLTDTEIKALLLNLFTAGTDT 306

Query: 388 TSLMVEWALVELIRHPHILAAAQKEL 465
           TS  VEWAL ELIRHP +L  AQKEL
Sbjct: 307 TSSTVEWALAELIRHPQLLHQAQKEL 332


>ref|NP_001064338.1| Os10g0320100 [Oryza sativa Japonica Group]
           gi|19881547|gb|AAM00948.1|AC021892_12 Putative flavonoid
           3'-hydroxylase [Oryza sativa Japonica Group]
           gi|22725927|gb|AAN04937.1| Putative chalcone flavonoid
           3' - hydroxylase [Oryza sativa Japonica Group]
           gi|31431083|gb|AAP52914.1| Flavonoid 3'-monooxygenase,
           putative, expressed [Oryza sativa Japonica Group]
           gi|113638947|dbj|BAF26252.1| Os10g0320100 [Oryza sativa
           Japonica Group] gi|125531442|gb|EAY78007.1| hypothetical
           protein OsI_33047 [Oryza sativa Indica Group]
           gi|125574353|gb|EAZ15637.1| hypothetical protein
           OsJ_31048 [Oryza sativa Japonica Group]
           gi|215693977|dbj|BAG89180.1| unnamed protein product
           [Oryza sativa Japonica Group]
           gi|340343700|gb|AEK31169.1| flavonoid 3'-hydroxylase
           [Oryza sativa Japonica Group]
          Length = 526

 Score =  130 bits (327), Expect = 1e-28
 Identities = 76/153 (49%), Positives = 97/153 (63%), Gaps = 2/153 (1%)
 Frame = +1

Query: 13  RVFEEDNGPDVEEFMKVLEENLKLATSMNIEDFIPWVKAWDVRGFVAKLKRLHRWYDGTL 192
           RVF  D G    EF +++ E ++LA   N+ DF+P ++  D +G VAK+KRLHR YD  +
Sbjct: 198 RVFAVDGGEGAREFKEMVVELMQLAGVFNVGDFVPALRWLDPQGVVAKMKRLHRRYDNMM 257

Query: 193 TKIIEEHKT--KPKIVSEGEAEGKDLLSVLLRLKEADVKDKETDNYKLTDTDIKALLTDL 366
              I E K   +P  V+ GE  G DLLSVLL   + + K  + D  K+T+TDIKALL +L
Sbjct: 258 NGFINERKAGAQPDGVAAGE-HGNDLLSVLLARMQEEQK-LDGDGEKITETDIKALLLNL 315

Query: 367 FIGGTDTTSLMVEWALVELIRHPHILAAAQKEL 465
           F  GTDTTS  VEWAL ELIRHP +L  AQ EL
Sbjct: 316 FTAGTDTTSSTVEWALAELIRHPDVLKEAQHEL 348