BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dioscorea21_contig00027408
         (1624 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAC98601.1| putative tRNA modification GTPase trmE [Oryza sa...   380   0.0  
ref|XP_002444316.1| hypothetical protein SORBIDRAFT_07g020060 [S...   375   0.0  
ref|NP_001149416.1| LOC100283042 [Zea mays] gi|195627080|gb|ACG3...   371   0.0  
gb|AFW62152.1| tRNA modification GTPase trmE [Zea mays]               370   0.0  
ref|XP_003574461.1| PREDICTED: tRNA modification GTPase MnmE-lik...   362   0.0  

>dbj|BAC98601.1| putative tRNA modification GTPase trmE [Oryza sativa Japonica
           Group]
          Length = 552

 Score =  380 bits (975), Expect(2) = 0.0
 Identities = 205/298 (68%), Positives = 230/298 (77%), Gaps = 15/298 (5%)
 Frame = +1

Query: 13  NPKSLIPRPRTLISRFSSTGKNLILTKDERA---GTSGTVE------------GSGTIAA 147
           +P++L  R   L SR S      +L  D+R+    T G+V+            G GTIAA
Sbjct: 36  SPRTLTTRAEALFSRHSH-----LLPADDRSPSPSTRGSVQPPPDYGGGGGGAGPGTIAA 90

Query: 148 IVTSLGGGPAAVGIVRLSGPMAVDVASRVFRPAKRNGEVPWRPRSHFVEYGLALDLEGNV 327
           IVTSLGGGPAAVGIVRLSGP A  VA RVFRPA+R    PWRPRSHFVEYG+ALD +G V
Sbjct: 91  IVTSLGGGPAAVGIVRLSGPDAAAVAGRVFRPARRAAP-PWRPRSHFVEYGVALDRDGGV 149

Query: 328 IDEVLAVPMLAPRSYTREDVVELQCHGSDVCLRRVLRACLEAGARLAEPGEFTLRAFLNG 507
           IDEVL VPMLAPRSYTREDVVELQCHG+D+CLRRVLRACLEAGARLA+PGEFT+RAFLNG
Sbjct: 150 IDEVLVVPMLAPRSYTREDVVELQCHGNDLCLRRVLRACLEAGARLADPGEFTVRAFLNG 209

Query: 508 RLDLSQAENIGRLIXXXXXXXXXXXXXGMQGGFSALVKGLRKQCIELLAEIEARLDFEDE 687
           RLDL+QAEN+ RLI             G+QGGFSALVK LR +CIELL EIEARLDFEDE
Sbjct: 210 RLDLAQAENVSRLISAKSAAAADSALAGIQGGFSALVKSLRSRCIELLTEIEARLDFEDE 269

Query: 688 LPALDSSMLIDKINLMWHDVQQALDTADYDKLLQSGLQIAIVGRPNVGKSSLLNAWSK 861
           LP LD +ML+ KIN M  +VQ ALDTA+YDKLLQSGLQ+AI+GRPNVGKSSLLNAWSK
Sbjct: 270 LPPLDLTMLVSKINGMRQEVQDALDTANYDKLLQSGLQVAIIGRPNVGKSSLLNAWSK 327



 Score =  333 bits (853), Expect(2) = 0.0
 Identities = 169/234 (72%), Positives = 201/234 (85%)
 Frame = +2

Query: 842  SSMHGVNIQSDRAIVTEIAGTTRDVVEAHVTVQGIPVTLLDTAGIRETDDLVEKIGVQRS 1021
            SS+     +S+RAIVTEIAGTTRDVVEA+V++ GIP+TLLDTAGIRETDD+VEKIGV+RS
Sbjct: 319  SSLLNAWSKSERAIVTEIAGTTRDVVEANVSIHGIPITLLDTAGIRETDDIVEKIGVERS 378

Query: 1022 EAAAMGADVIIMTISASDGWTEDDKKLMEHLQMNQMSTSFATPMILVINKIDCAPSITEE 1201
            EAAA+GAD+IIMTISA DGWTEDD KL+EH+ +++ S+    PM+LVINK+DCAP I+ E
Sbjct: 379  EAAALGADLIIMTISAVDGWTEDDTKLIEHVLISKKSSGAPVPMVLVINKVDCAPFISGE 438

Query: 1202 LFEINTRVFKKHVCTCAVTGQGIVELEKAVLEVRGLDSIPAGGRRWTVNQRQCEQLLRTR 1381
             FE    VFKKHV TCAVTG+GI ELEKA++EVRGL+ +P+ GRRWTVNQRQ EQLLRT+
Sbjct: 439  QFEQFHGVFKKHVQTCAVTGKGISELEKAIIEVRGLEPVPSEGRRWTVNQRQFEQLLRTQ 498

Query: 1382 EALSRLKSSICEELPSDFWTIDLREAVLALGEISGVDISEEVLSNIFSKFCIGK 1543
            +A +RL+SSI E+LP DFWTIDLREA LAL  ISG DISEEVLS+IFSKFCIGK
Sbjct: 499  QAFTRLESSINEQLPMDFWTIDLREAALALATISGEDISEEVLSSIFSKFCIGK 552


>ref|XP_002444316.1| hypothetical protein SORBIDRAFT_07g020060 [Sorghum bicolor]
           gi|241940666|gb|EES13811.1| hypothetical protein
           SORBIDRAFT_07g020060 [Sorghum bicolor]
          Length = 548

 Score =  375 bits (963), Expect(2) = 0.0
 Identities = 205/302 (67%), Positives = 226/302 (74%), Gaps = 19/302 (6%)
 Frame = +1

Query: 13  NPKSLIPRPRTLISRFSSTGKNLILTKDERA------------------GTSGTVEGSGT 138
           NP S    PRTL +R  +    L+L  DER+                  G  G     GT
Sbjct: 30  NPSS----PRTLATRADT----LVLPGDERSPSPSPSPSRLPGQLRPDYGGGGGGGAPGT 81

Query: 139 IAAIVTSLGGGPAAVGIVRLSGPMAVDVASRVFRPA-KRNGEVPWRPRSHFVEYGLALDL 315
           IAAIVTSLGGGPAAVGIVRLSGP AV VA RVFRPA  R    PW+PRSHFVEYGLALD 
Sbjct: 82  IAAIVTSLGGGPAAVGIVRLSGPDAVAVADRVFRPAGARRASAPWQPRSHFVEYGLALDA 141

Query: 316 EGNVIDEVLAVPMLAPRSYTREDVVELQCHGSDVCLRRVLRACLEAGARLAEPGEFTLRA 495
           +G+VIDEVL VPMLAPRSYTREDVVELQCHG+D+CLRRVLRACLEAGARLA+PGEFTLRA
Sbjct: 142 DGSVIDEVLVVPMLAPRSYTREDVVELQCHGNDLCLRRVLRACLEAGARLADPGEFTLRA 201

Query: 496 FLNGRLDLSQAENIGRLIXXXXXXXXXXXXXGMQGGFSALVKGLRKQCIELLAEIEARLD 675
           FLNGRLDL+QAEN+ RLI             G+QGGFS LV+ LR +CIELL EIEARLD
Sbjct: 202 FLNGRLDLAQAENVSRLISAKSAAVADSALAGIQGGFSTLVRSLRSRCIELLTEIEARLD 261

Query: 676 FEDELPALDSSMLIDKINLMWHDVQQALDTADYDKLLQSGLQIAIVGRPNVGKSSLLNAW 855
           FEDE+P LD  ML+ KIN M  +VQ ALDT++YDKLLQSGLQIAI+GRPNVGKSSLLNAW
Sbjct: 262 FEDEMPPLDLVMLVSKINCMRQEVQDALDTSNYDKLLQSGLQIAIIGRPNVGKSSLLNAW 321

Query: 856 SK 861
           SK
Sbjct: 322 SK 323



 Score =  327 bits (839), Expect(2) = 0.0
 Identities = 165/234 (70%), Positives = 198/234 (84%)
 Frame = +2

Query: 842  SSMHGVNIQSDRAIVTEIAGTTRDVVEAHVTVQGIPVTLLDTAGIRETDDLVEKIGVQRS 1021
            SS+     +S+RAIVTEIAGTTRDVVEA+V++ G+PVTLLDTAGIRETDD+VEKIGV+RS
Sbjct: 315  SSLLNAWSKSERAIVTEIAGTTRDVVEANVSIHGVPVTLLDTAGIRETDDVVEKIGVKRS 374

Query: 1022 EAAAMGADVIIMTISASDGWTEDDKKLMEHLQMNQMSTSFATPMILVINKIDCAPSITEE 1201
            EAAAMGAD+I+M ISA DGWT+DD KLMEH+ +N+ S+  A PM+LVINK+DCAP +  E
Sbjct: 375  EAAAMGADLIVMAISAVDGWTDDDTKLMEHVLINRKSSGSAVPMVLVINKVDCAPFVPGE 434

Query: 1202 LFEINTRVFKKHVCTCAVTGQGIVELEKAVLEVRGLDSIPAGGRRWTVNQRQCEQLLRTR 1381
             FE  + +F KHV TCAVTG+GI +LE AV+EVRG++ +P+ GRRWTVNQRQ EQLLRT+
Sbjct: 435  QFEQFSGLFIKHVQTCAVTGKGISDLESAVIEVRGIEHVPSEGRRWTVNQRQFEQLLRTK 494

Query: 1382 EALSRLKSSICEELPSDFWTIDLREAVLALGEISGVDISEEVLSNIFSKFCIGK 1543
            EA  RL+SSI E+LP DFWT+DLREA LAL  ISG DISEEVLS+IFSKFCIGK
Sbjct: 495  EAFLRLESSINEQLPMDFWTVDLREAALALATISGEDISEEVLSSIFSKFCIGK 548


>ref|NP_001149416.1| LOC100283042 [Zea mays] gi|195627080|gb|ACG35370.1| tRNA
           modification GTPase trmE [Zea mays]
          Length = 546

 Score =  371 bits (953), Expect(2) = 0.0
 Identities = 202/300 (67%), Positives = 224/300 (74%), Gaps = 17/300 (5%)
 Frame = +1

Query: 13  NPKSLIPRPRTLISRFSSTGKNLILTKDERA----------------GTSGTVEGSGTIA 144
           NP S    PRTL  R  +    L+L  DER                 G+ G      TIA
Sbjct: 30  NPSS----PRTLAVRVDT----LVLPGDERLPSPSPPRLPNQLRPDDGSGGGGGAPSTIA 81

Query: 145 AIVTSLGGGPAAVGIVRLSGPMAVDVASRVFRPA-KRNGEVPWRPRSHFVEYGLALDLEG 321
           AIVTSLGGGPAAVGIVR+SGP AV VA RVFRPA  R    PW+PRSHFVEYGLALD +G
Sbjct: 82  AIVTSLGGGPAAVGIVRISGPDAVAVADRVFRPAGTRRASTPWQPRSHFVEYGLALDADG 141

Query: 322 NVIDEVLAVPMLAPRSYTREDVVELQCHGSDVCLRRVLRACLEAGARLAEPGEFTLRAFL 501
           +VIDEVL VPML+PRSYTREDVVELQCHG+D+CLRR+LRACLEAGARLA+PGEFTLRAFL
Sbjct: 142 SVIDEVLVVPMLSPRSYTREDVVELQCHGNDLCLRRLLRACLEAGARLADPGEFTLRAFL 201

Query: 502 NGRLDLSQAENIGRLIXXXXXXXXXXXXXGMQGGFSALVKGLRKQCIELLAEIEARLDFE 681
           NGRLDL+QAEN+ RLI             G+QGGFS LVK LR +CIELL EIEARLDFE
Sbjct: 202 NGRLDLAQAENVSRLISAKSAAAADSALAGIQGGFSTLVKSLRSRCIELLTEIEARLDFE 261

Query: 682 DELPALDSSMLIDKINLMWHDVQQALDTADYDKLLQSGLQIAIVGRPNVGKSSLLNAWSK 861
           DE+P LD  ML+ KIN M  +VQ ALDT++YDKLLQSGLQIAI+GRPNVGKSSLLNAWSK
Sbjct: 262 DEMPPLDPVMLVSKINCMRKEVQDALDTSNYDKLLQSGLQIAIIGRPNVGKSSLLNAWSK 321



 Score =  330 bits (845), Expect(2) = 0.0
 Identities = 164/234 (70%), Positives = 200/234 (85%)
 Frame = +2

Query: 842  SSMHGVNIQSDRAIVTEIAGTTRDVVEAHVTVQGIPVTLLDTAGIRETDDLVEKIGVQRS 1021
            SS+     +S+RAIVTEIAGTTRDVVEA+V++ G+PVTLLDTAGIRETDD+VEKIGV+RS
Sbjct: 313  SSLLNAWSKSERAIVTEIAGTTRDVVEANVSIHGVPVTLLDTAGIRETDDVVEKIGVKRS 372

Query: 1022 EAAAMGADVIIMTISASDGWTEDDKKLMEHLQMNQMSTSFATPMILVINKIDCAPSITEE 1201
            EAAAMGAD+I+M ISA DGWT+DD KLMEH+ +N+ S+  A PM+LVINK+DCAP +  E
Sbjct: 373  EAAAMGADLIVMAISAVDGWTDDDTKLMEHVSVNRKSSGSAVPMVLVINKVDCAPFVPGE 432

Query: 1202 LFEINTRVFKKHVCTCAVTGQGIVELEKAVLEVRGLDSIPAGGRRWTVNQRQCEQLLRTR 1381
             F+  + +F+KHV TCAVTG+GI +LE AV+EVRG++ +P+ GRRWTVNQRQ EQLLRT+
Sbjct: 433  QFKQFSGLFRKHVQTCAVTGKGISDLESAVIEVRGIEHVPSEGRRWTVNQRQFEQLLRTK 492

Query: 1382 EALSRLKSSICEELPSDFWTIDLREAVLALGEISGVDISEEVLSNIFSKFCIGK 1543
            EA +RL+SSI E+LP DFWT+DLREA LAL  ISG DISEEVLS+IFSKFCIGK
Sbjct: 493  EAFARLESSINEQLPMDFWTVDLREAALALATISGEDISEEVLSSIFSKFCIGK 546


>gb|AFW62152.1| tRNA modification GTPase trmE [Zea mays]
          Length = 545

 Score =  370 bits (950), Expect(2) = 0.0
 Identities = 190/253 (75%), Positives = 209/253 (82%), Gaps = 1/253 (0%)
 Frame = +1

Query: 106 GTSGTVEGSGTIAAIVTSLGGGPAAVGIVRLSGPMAVDVASRVFRPA-KRNGEVPWRPRS 282
           G+ G      TIAAIVTSLGGGPAAVGIVR+SGP AV VA RVFRPA  R    PW+PRS
Sbjct: 68  GSGGGGGAPSTIAAIVTSLGGGPAAVGIVRISGPDAVAVADRVFRPAGTRRASTPWQPRS 127

Query: 283 HFVEYGLALDLEGNVIDEVLAVPMLAPRSYTREDVVELQCHGSDVCLRRVLRACLEAGAR 462
           HFVEYGLALD +G+VIDEVL VPML+PRSYTREDVVELQCHG+D+CLRRVLRACLEAGAR
Sbjct: 128 HFVEYGLALDADGSVIDEVLVVPMLSPRSYTREDVVELQCHGNDLCLRRVLRACLEAGAR 187

Query: 463 LAEPGEFTLRAFLNGRLDLSQAENIGRLIXXXXXXXXXXXXXGMQGGFSALVKGLRKQCI 642
           LA+PGEFTLRAFLNGRLDL+QAEN+ RLI             G+QGGFS LVK LR +CI
Sbjct: 188 LADPGEFTLRAFLNGRLDLAQAENVSRLISAKSAAAADSALAGIQGGFSTLVKSLRSRCI 247

Query: 643 ELLAEIEARLDFEDELPALDSSMLIDKINLMWHDVQQALDTADYDKLLQSGLQIAIVGRP 822
           ELL EIEARLDFEDE+P LD  ML+ KIN M  +VQ ALDT++YDKLLQSGLQIAI+GRP
Sbjct: 248 ELLTEIEARLDFEDEMPPLDPVMLVSKINCMRKEVQDALDTSNYDKLLQSGLQIAIIGRP 307

Query: 823 NVGKSSLLNAWSK 861
           NVGKSSLLNAWSK
Sbjct: 308 NVGKSSLLNAWSK 320



 Score =  330 bits (845), Expect(2) = 0.0
 Identities = 164/234 (70%), Positives = 200/234 (85%)
 Frame = +2

Query: 842  SSMHGVNIQSDRAIVTEIAGTTRDVVEAHVTVQGIPVTLLDTAGIRETDDLVEKIGVQRS 1021
            SS+     +S+RAIVTEIAGTTRDVVEA+V++ G+PVTLLDTAGIRETDD+VEKIGV+RS
Sbjct: 312  SSLLNAWSKSERAIVTEIAGTTRDVVEANVSINGVPVTLLDTAGIRETDDVVEKIGVKRS 371

Query: 1022 EAAAMGADVIIMTISASDGWTEDDKKLMEHLQMNQMSTSFATPMILVINKIDCAPSITEE 1201
            EAAAMGAD+I+M ISA DGWT+DD KLMEH+ +N+ S+  A PM+LVINK+DCAP +  E
Sbjct: 372  EAAAMGADLIVMAISAVDGWTDDDTKLMEHVSVNRKSSGSAVPMVLVINKVDCAPFVPGE 431

Query: 1202 LFEINTRVFKKHVCTCAVTGQGIVELEKAVLEVRGLDSIPAGGRRWTVNQRQCEQLLRTR 1381
             F+  + +F+KHV TCAVTG+GI +LE AV+EVRG++ +P+ GRRWTVNQRQ EQLLRT+
Sbjct: 432  QFKQFSGLFRKHVQTCAVTGKGISDLESAVIEVRGIEHVPSEGRRWTVNQRQFEQLLRTK 491

Query: 1382 EALSRLKSSICEELPSDFWTIDLREAVLALGEISGVDISEEVLSNIFSKFCIGK 1543
            EA +RL+SSI E+LP DFWT+DLREA LAL  ISG DISEEVLS+IFSKFCIGK
Sbjct: 492  EAFARLESSINEQLPMDFWTVDLREAALALATISGEDISEEVLSSIFSKFCIGK 545


>ref|XP_003574461.1| PREDICTED: tRNA modification GTPase MnmE-like [Brachypodium
           distachyon]
          Length = 543

 Score =  362 bits (929), Expect(2) = 0.0
 Identities = 197/296 (66%), Positives = 222/296 (75%), Gaps = 13/296 (4%)
 Frame = +1

Query: 13  NPKSLIPRPRTLISRFSSTGKNLILTKDERAGTS-------------GTVEGSGTIAAIV 153
           +PK+L  RP  L S      ++L+L  DER   S             G     GTIAAIV
Sbjct: 29  SPKTLTTRPGALRSI-----QSLVLPGDERLPPSSPSRRAAQPPLDYGGGGSPGTIAAIV 83

Query: 154 TSLGGGPAAVGIVRLSGPMAVDVASRVFRPAKRNGEVPWRPRSHFVEYGLALDLEGNVID 333
           TSLGGGPAAVG+VRLSG  AV VA RVFRPA++  E  W+PRSHFVEYG ALD +G+VID
Sbjct: 84  TSLGGGPAAVGVVRLSGADAVAVAGRVFRPARKEPEA-WKPRSHFVEYGFALDGDGSVID 142

Query: 334 EVLAVPMLAPRSYTREDVVELQCHGSDVCLRRVLRACLEAGARLAEPGEFTLRAFLNGRL 513
           EVL VPMLAPRSYTREDVVELQCHG+D+CLRRVL ACLEAGARLA+PGEFTLRAFLNGRL
Sbjct: 143 EVLVVPMLAPRSYTREDVVELQCHGNDLCLRRVLGACLEAGARLADPGEFTLRAFLNGRL 202

Query: 514 DLSQAENIGRLIXXXXXXXXXXXXXGMQGGFSALVKGLRKQCIELLAEIEARLDFEDELP 693
           DL+QAEN+ RLI             G+QGGFS LVK LR +CIE L EIEARLDFEDE+P
Sbjct: 203 DLAQAENVSRLISAKSTAAADSALAGIQGGFSTLVKSLRSRCIEFLTEIEARLDFEDEMP 262

Query: 694 ALDSSMLIDKINLMWHDVQQALDTADYDKLLQSGLQIAIVGRPNVGKSSLLNAWSK 861
            LD  ML+ +I  M  +VQ ALDTA+YD+LLQSGLQIAI+GRPNVGKSSLLNAWSK
Sbjct: 263 PLDPMMLVSQITSMRQEVQDALDTANYDRLLQSGLQIAIIGRPNVGKSSLLNAWSK 318



 Score =  331 bits (849), Expect(2) = 0.0
 Identities = 167/234 (71%), Positives = 199/234 (85%)
 Frame = +2

Query: 842  SSMHGVNIQSDRAIVTEIAGTTRDVVEAHVTVQGIPVTLLDTAGIRETDDLVEKIGVQRS 1021
            SS+     +S+RAIVTEIAGTTRDVVEA+V++ GIPVTLLDTAGIRETDD+VEKIGV+RS
Sbjct: 310  SSLLNAWSKSERAIVTEIAGTTRDVVEANVSIHGIPVTLLDTAGIRETDDIVEKIGVERS 369

Query: 1022 EAAAMGADVIIMTISASDGWTEDDKKLMEHLQMNQMSTSFATPMILVINKIDCAPSITEE 1201
            EAAA+GAD++IMTISA DGWTEDD KL+EH+  ++  +  A PM+LVINK+DC P ++ E
Sbjct: 370  EAAALGADMVIMTISAVDGWTEDDTKLIEHVMNDKKLSGSAVPMVLVINKVDCKPFVSGE 429

Query: 1202 LFEINTRVFKKHVCTCAVTGQGIVELEKAVLEVRGLDSIPAGGRRWTVNQRQCEQLLRTR 1381
             FE  + +F+KHV TCAVTG+GI ELE+AV+EVRGL+ IP GGRRWTVNQRQ EQLLRT+
Sbjct: 430  QFEKFSGIFRKHVHTCAVTGKGISELERAVIEVRGLEPIPPGGRRWTVNQRQLEQLLRTQ 489

Query: 1382 EALSRLKSSICEELPSDFWTIDLREAVLALGEISGVDISEEVLSNIFSKFCIGK 1543
            EA  RL+SSI E+LP DFWTIDLREA LAL  I+G DISEEVLS+IFSKFCIGK
Sbjct: 490  EAFKRLESSINEQLPMDFWTIDLREAALALATINGEDISEEVLSSIFSKFCIGK 543