BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dioscorea21_contig00026968
         (1236 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002516192.1| Pectinesterase PPE8B precursor, putative [Ri...   374   e-101
dbj|BAH56489.1| pectin methylesterase 1 [Prunus persica]              372   e-100
ref|XP_002326320.1| predicted protein [Populus trichocarpa] gi|2...   365   1e-98
ref|XP_002878961.1| pectinesterase family protein [Arabidopsis l...   358   2e-96
ref|NP_180212.1| pectinesterase 12 [Arabidopsis thaliana] gi|753...   347   3e-93

>ref|XP_002516192.1| Pectinesterase PPE8B precursor, putative [Ricinus communis]
            gi|223544678|gb|EEF46194.1| Pectinesterase PPE8B
            precursor, putative [Ricinus communis]
          Length = 543

 Score =  374 bits (961), Expect = e-101
 Identities = 210/387 (54%), Positives = 258/387 (66%), Gaps = 17/387 (4%)
 Frame = +2

Query: 125  MQTSLL-LILLATFFTP-------------SLSLNATDDHNLLTIKSLCQTTPYPNSCFD 262
            M  SLL L+LL  FF+P             S S N+ D H L ++++ C   PYP++CFD
Sbjct: 1    MAFSLLNLLLLLFFFSPIFFSSAWAANISSSSSANSLDTH-LSSLRNFCSGRPYPDACFD 59

Query: 263  ALKXXXXXXXXXXXXXXXXQTLNTAXXXXXXXXXXXXXXXXXXXXXQK--GSLQDCQELH 436
            +LK                QTL TA                     QK  G++QDC+ELH
Sbjct: 60   SLKLSISINISPNILNYILQTLQTAISEAGKLTNLFSSAGSSSNIIQKQRGTIQDCKELH 119

Query: 437  QITLT-YXXXXXXXXXXXXXXXXXVRSHLSAALTNKATCLEGLATASGPSKQALVSSLSS 613
            +ITL+                    R++LSAALTNK TCLEGL +ASGP K  LV+S+ S
Sbjct: 120  EITLSSLKRSVSRIQAGDSQKLADARAYLSAALTNKNTCLEGLDSASGPLKPVLVNSVIS 179

Query: 614  TYAHVTNSLSIISXXXXXXXXXXXXLSGKLPKWLTRKDRRLLQSDDDYNNEYDPASILTV 793
            TY HV+NS+S+I             L G  PKWL++KDRR+LQSDD   +EYDP+ +L V
Sbjct: 180  TYKHVSNSISMIPSPNSEKGQKNRRLLG-FPKWLSKKDRRILQSDD--GDEYDPSEVLVV 236

Query: 794  AADGTGNFTTISDAIAFAPNNSDYRTVIIVRAGVYDEHVEIPSYKPNIVLLGEGSDVTII 973
            AADGTG+FTTI+DAI FAP NS  R +I V+ GVY+E+VEIPSYK NIVL+G+GSDVT I
Sbjct: 237  AADGTGSFTTITDAINFAPKNSFDRIIISVKEGVYEENVEIPSYKTNIVLIGDGSDVTFI 296

Query: 974  TGNRSVADGWTTFRSATVAVSGQGFLARDLTIMNTAGAVKNQAVALRVNADLSAVYRCKI 1153
            TGNRSV DGWTTFRSAT+AVSG+GFLARD+TI NTAGA K+QAVALR+NADL+A+YRC I
Sbjct: 297  TGNRSVDDGWTTFRSATLAVSGEGFLARDITIENTAGAQKHQAVALRINADLAAMYRCTI 356

Query: 1154 DGYQDTLYVHSFRQFYRECDIFGTVDF 1234
            +GYQDTLYVHSFRQFYRECDI+GT+D+
Sbjct: 357  NGYQDTLYVHSFRQFYRECDIYGTIDY 383


>dbj|BAH56489.1| pectin methylesterase 1 [Prunus persica]
          Length = 543

 Score =  372 bits (954), Expect = e-100
 Identities = 201/358 (56%), Positives = 248/358 (69%), Gaps = 4/358 (1%)
 Frame = +2

Query: 173  SLSLNATD-DHNLLTIKSLCQTTPYPNSCFDALKXXXXXXXXXXXXXXXXQTLNTAXXXX 349
            S S NAT+ + NL +I+S C++TPYP+ CFD+LK                Q+L  A    
Sbjct: 30   SSSTNATNFNPNLSSIRSFCKSTPYPDVCFDSLKLSISINISPNIITFLLQSLQVAISEA 89

Query: 350  XXXXXXXXXXXXXXXXX--QKGSLQDCQELHQITLT-YXXXXXXXXXXXXXXXXXVRSHL 520
                               QKG++QDC+ELHQITL+                    R++L
Sbjct: 90   GKLSDLFYKAGRYSNIVEKQKGAIQDCKELHQITLSSLQRSVSRVRAGNTKKLNDARAYL 149

Query: 521  SAALTNKATCLEGLATASGPSKQALVSSLSSTYAHVTNSLSIISXXXXXXXXXXXXLSGK 700
            SAALTNK TCLEGL +ASGP K ALV+SL+STY +V+NSLS+IS            L   
Sbjct: 150  SAALTNKNTCLEGLDSASGPMKPALVNSLTSTYKYVSNSLSVISKPGAPKGGTNRHLLA- 208

Query: 701  LPKWLTRKDRRLLQSDDDYNNEYDPASILTVAADGTGNFTTISDAIAFAPNNSDYRTVII 880
            +P W++RKDRR+L+S  D   +YDP+ +LTVAADGTGNFTTI+DA+ FAPNNS  RT+I 
Sbjct: 209  VPTWMSRKDRRILESSGD---KYDPSEVLTVAADGTGNFTTITDAVNFAPNNSYDRTIIY 265

Query: 881  VRAGVYDEHVEIPSYKPNIVLLGEGSDVTIITGNRSVADGWTTFRSATVAVSGQGFLARD 1060
            V+ GVY E+VEIPSYK NIVLLG+G D+T+ITGNRSV DGWTTFRSAT+AVSG+GFLARD
Sbjct: 266  VKEGVYVENVEIPSYKTNIVLLGDGRDITVITGNRSVVDGWTTFRSATLAVSGEGFLARD 325

Query: 1061 LTIMNTAGAVKNQAVALRVNADLSAVYRCKIDGYQDTLYVHSFRQFYRECDIFGTVDF 1234
            +T  NTAG  K+QAVALRVNAD +A+Y+C I+GYQDTLYVHSFRQFYRECDIFGT+D+
Sbjct: 326  ITFENTAGPEKHQAVALRVNADFAAIYKCIINGYQDTLYVHSFRQFYRECDIFGTIDY 383


>ref|XP_002326320.1| predicted protein [Populus trichocarpa] gi|222833513|gb|EEE71990.1|
            predicted protein [Populus trichocarpa]
          Length = 541

 Score =  365 bits (938), Expect = 1e-98
 Identities = 201/372 (54%), Positives = 249/372 (66%), Gaps = 6/372 (1%)
 Frame = +2

Query: 137  LLLILLATFFTPSLSLNATD----DHNLLTIKSLCQTTPYPNSCFDALKXXXXXXXXXXX 304
            LLL++ + FFT + +LN +     D +L TI++ C + PYP++CF++LK           
Sbjct: 9    LLLLISSVFFTSTWALNTSSTTSLDAHLSTIRTFCNSRPYPDACFNSLKLSISINISPNI 68

Query: 305  XXXXXQTLNTAXXXXXXXXXXXXXXXXXXXXX-QKGSLQDCQELHQITLT-YXXXXXXXX 478
                 QTL TA                      Q+G++QDC ELHQIT++          
Sbjct: 69   INLLLQTLQTAISEAGKLTNLFSIAGGSNIIERQRGTIQDCLELHQITVSSLQRSVSRVR 128

Query: 479  XXXXXXXXXVRSHLSAALTNKATCLEGLATASGPSKQALVSSLSSTYAHVTNSLSIISXX 658
                      R++LSAA+TNK TCLEGL +ASGP K AL++SL+STY HVTNSLS++   
Sbjct: 129  AGDSRKLVDARAYLSAAVTNKNTCLEGLDSASGPLKPALLNSLTSTYQHVTNSLSMLPKS 188

Query: 659  XXXXXXXXXXLSGKLPKWLTRKDRRLLQSDDDYNNEYDPASILTVAADGTGNFTTISDAI 838
                           PKW+++KDRR+LQSD+D   EYDP+  L VAADGTGNF+TI+DAI
Sbjct: 189  KHSKQGYKNRRLLGFPKWMSKKDRRILQSDED---EYDPSEELIVAADGTGNFSTITDAI 245

Query: 839  AFAPNNSDYRTVIIVRAGVYDEHVEIPSYKPNIVLLGEGSDVTIITGNRSVADGWTTFRS 1018
             FAPNNS  R +I VR GVY E+VEIP YK NIVLLG+G+DVT ITGNRSV DGWTTFRS
Sbjct: 246  NFAPNNSYDRIIIRVREGVYAENVEIPIYKTNIVLLGDGTDVTFITGNRSVVDGWTTFRS 305

Query: 1019 ATVAVSGQGFLARDLTIMNTAGAVKNQAVALRVNADLSAVYRCKIDGYQDTLYVHSFRQF 1198
            AT+AVSG GFLA D+TI NTAG  K+QAVALRV+ADL A+YRC I+GYQDTLYVHSFRQF
Sbjct: 306  ATLAVSGDGFLALDITIDNTAGPEKHQAVALRVSADLVALYRCSINGYQDTLYVHSFRQF 365

Query: 1199 YRECDIFGTVDF 1234
            YRECDI GT+D+
Sbjct: 366  YRECDISGTIDY 377


>ref|XP_002878961.1| pectinesterase family protein [Arabidopsis lyrata subsp. lyrata]
            gi|297324800|gb|EFH55220.1| pectinesterase family protein
            [Arabidopsis lyrata subsp. lyrata]
          Length = 542

 Score =  358 bits (919), Expect = 2e-96
 Identities = 197/378 (52%), Positives = 239/378 (63%), Gaps = 11/378 (2%)
 Frame = +2

Query: 134  SLLLILLATFFTPSLSLNATDDHNLLTIKSLCQTTPYPNSCFDALKXXXXXXXXXXXXXX 313
            S L  LL T    S SL      N  ++ S C  TPYPN+CFD+LK              
Sbjct: 9    SFLFFLLFTPLVVSTSLKP----NQTSLTSFCMNTPYPNACFDSLKLSISINISPNILSF 64

Query: 314  XXQTLNTAXXXXXXXXXXXXXXXXXXXXX--QKGSLQDCQELHQITLTYXXXXXXXXXXX 487
              QTL TA                       Q+GSLQDC++LHQIT ++           
Sbjct: 65   LLQTLQTALSEAGKLTDLLSGAGISNNLVEGQRGSLQDCKDLHQITSSFLKRSISKIQDG 124

Query: 488  XXXXXXV---RSHLSAALTNKATCLEGLATASGPSKQALVSSLSSTYAHVTNSLSII--- 649
                  +   R++LSAALTNK TCLEGL TASGP K  LV+S   TY HV+NSLS +   
Sbjct: 125  VNDSRKLADARAYLSAALTNKITCLEGLDTASGPLKPKLVTSFMETYKHVSNSLSALPKQ 184

Query: 650  ---SXXXXXXXXXXXXLSGKLPKWLTRKDRRLLQSDDDYNNEYDPASILTVAADGTGNFT 820
               +            L G LP W+++KD R L+   D  +EYDP+ I+ VAADGTGNF+
Sbjct: 185  RRATNLKTDGKTKNRRLFGLLPDWVSKKDHRFLEDSSDGYDEYDPSEIIVVAADGTGNFS 244

Query: 821  TISDAIAFAPNNSDYRTVIIVRAGVYDEHVEIPSYKPNIVLLGEGSDVTIITGNRSVADG 1000
            TI++AI+FAP+ S+ R +I VR GVYDE++EIP YK NIVL+G+GSDVT ITGNRSV DG
Sbjct: 245  TINEAISFAPDMSNDRVLIYVREGVYDENIEIPIYKTNIVLIGDGSDVTFITGNRSVGDG 304

Query: 1001 WTTFRSATVAVSGQGFLARDLTIMNTAGAVKNQAVALRVNADLSAVYRCKIDGYQDTLYV 1180
            WTTFRSAT+AVSG+GFLARD+ I NTAG  K+QAVALRVNAD  A+YRC IDGYQDTLY 
Sbjct: 305  WTTFRSATLAVSGEGFLARDMMITNTAGPEKHQAVALRVNADFVALYRCVIDGYQDTLYT 364

Query: 1181 HSFRQFYRECDIFGTVDF 1234
            HSFRQFYRECDI+GT+D+
Sbjct: 365  HSFRQFYRECDIYGTIDY 382


>ref|NP_180212.1| pectinesterase 12 [Arabidopsis thaliana]
            gi|75318311|sp|O48711.1|PME12_ARATH RecName:
            Full=Probable pectinesterase/pectinesterase inhibitor 12;
            Includes: RecName: Full=Pectinesterase inhibitor 12;
            AltName: Full=Pectin methylesterase inhibitor 12;
            Includes: RecName: Full=Pectinesterase 12; Short=PE 12;
            AltName: Full=Pectin methylesterase 12; Short=AtPME12;
            Flags: Precursor gi|2739369|gb|AAC14493.1| putative
            pectinesterase [Arabidopsis thaliana]
            gi|18176445|gb|AAL60045.1| putative pectinesterase
            [Arabidopsis thaliana] gi|21689727|gb|AAM67485.1|
            putative pectinesterase [Arabidopsis thaliana]
            gi|330252745|gb|AEC07839.1| pectinesterase 12
            [Arabidopsis thaliana]
          Length = 547

 Score =  347 bits (891), Expect = 3e-93
 Identities = 192/381 (50%), Positives = 242/381 (63%), Gaps = 14/381 (3%)
 Frame = +2

Query: 134  SLLLILLATFFTPSL---SLNATDDHNLLTIKSLCQTTPYPNSCFDALKXXXXXXXXXXX 304
            S LL LL  FFTPS+   S   + + +  +  S C+ TPYP++CF +LK           
Sbjct: 9    SSLLFLL--FFTPSVFSYSYQPSLNPHETSATSFCKNTPYPDACFTSLKLSISINISPNI 66

Query: 305  XXXXXQTLNTAXXXXXXXXXXXXXXXXXXXXX--QKGSLQDCQELHQITLTYXXXXXXXX 478
                 QTL TA                       Q+GSLQDC++LH IT ++        
Sbjct: 67   LSFLLQTLQTALSEAGKLTDLLSGAGVSNNLVEGQRGSLQDCKDLHHITSSFLKRSISKI 126

Query: 479  XXXXXXXXXV---RSHLSAALTNKATCLEGLATASGPSKQALVSSLSSTYAHVTNSLSII 649
                     +   R++LSAALTNK TCLEGL +ASGP K  LV+S ++TY H++NSLS +
Sbjct: 127  QDGVNDSRKLADARAYLSAALTNKITCLEGLESASGPLKPKLVTSFTTTYKHISNSLSAL 186

Query: 650  ------SXXXXXXXXXXXXLSGKLPKWLTRKDRRLLQSDDDYNNEYDPASILTVAADGTG 811
                  +            L G  P W+ +KD R L+   D  +EYDP+  L VAADGTG
Sbjct: 187  PKQRRTTNPKTGGNTKNRRLLGLFPDWVYKKDHRFLEDSSDGYDEYDPSESLVVAADGTG 246

Query: 812  NFTTISDAIAFAPNNSDYRTVIIVRAGVYDEHVEIPSYKPNIVLLGEGSDVTIITGNRSV 991
            NF+TI++AI+FAPN S+ R +I V+ GVYDE+++IP YK NIVL+G+GSDVT ITGNRSV
Sbjct: 247  NFSTINEAISFAPNMSNDRVLIYVKEGVYDENIDIPIYKTNIVLIGDGSDVTFITGNRSV 306

Query: 992  ADGWTTFRSATVAVSGQGFLARDLTIMNTAGAVKNQAVALRVNADLSAVYRCKIDGYQDT 1171
             DGWTTFRSAT+AVSG+GFLARD+ I NTAG  K+QAVALRVNAD  A+YRC IDGYQDT
Sbjct: 307  GDGWTTFRSATLAVSGEGFLARDIMITNTAGPEKHQAVALRVNADFVALYRCVIDGYQDT 366

Query: 1172 LYVHSFRQFYRECDIFGTVDF 1234
            LY HSFRQFYRECDI+GT+D+
Sbjct: 367  LYTHSFRQFYRECDIYGTIDY 387