BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dioscorea21_contig00026770
         (1392 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002319761.1| flavonoid 3'-hydroxylase [Populus trichocarp...   467   e-144
gb|ADE80941.1| flavonoid 3'-hydroxylase [Epimedium sagittatum]        468   e-143
gb|AAG49298.1|AF313488_1 putative flavonoid 3'-hydroxylase [Call...   472   e-143
gb|AGC29948.1| CYP75B65 [Sinopodophyllum hexandrum]                   459   e-142
gb|ADM26615.1| flavonoid 3'-hydroxylase [Cynara cardunculus var....   464   e-142

>ref|XP_002319761.1| flavonoid 3'-hydroxylase [Populus trichocarpa]
            gi|222858137|gb|EEE95684.1| flavonoid 3'-hydroxylase
            [Populus trichocarpa]
          Length = 521

 Score =  467 bits (1202), Expect(2) = e-144
 Identities = 225/370 (60%), Positives = 283/370 (76%), Gaps = 15/370 (4%)
 Frame = +1

Query: 1    PPNSGAEHVAYNYQDLVFAPYGPQWRMFRRLCSLHLFSTKVLDELRHVREDEVTLLVTGL 180
            PPNSGA+H+AYNYQDLVFAPYGP+WRM R++ S+HLFS K LD+ RH+R++EV +L   L
Sbjct: 100  PPNSGAKHIAYNYQDLVFAPYGPRWRMLRKISSVHLFSAKSLDDFRHIRQEEVAVLTGAL 159

Query: 181  RRA-EKVDVEVGKEINICVTNALSKAMLGRKVFDDGDNG------EFKDLVVELMKLSGS 339
             R+     V +G+ +N+C  NAL + MLGR+VF DG         EFK +VVE+M L+G 
Sbjct: 160  TRSGPTTPVNLGQLLNVCTANALGRVMLGRRVFGDGSGDGDPKADEFKSMVVEVMVLAGV 219

Query: 340  FNVGDFVPWLKPLDVHGVVSRMKKIHKWYDDFLNKIIEDHNKEGGRG--------DLLSV 495
            FN+GDFVP L+ LD+ GV ++MKK+HK +D FL  I+E+H               DLLS 
Sbjct: 220  FNIGDFVPALEWLDLQGVAAKMKKLHKRFDAFLTNIVEEHKTSSSTASVRSEKHTDLLST 279

Query: 496  LLRLKEDHDNNDEEIKLTDTNIKALLLDLFQAGTDTTSSTVEWGLAELLRHPDILTAAQN 675
            L+ LKE  D + EE KLTDT IKALLL++F AGTDT+SSTVEW +AEL+RHPDIL   + 
Sbjct: 280  LIALKEQQDVDGEEGKLTDTEIKALLLNMFTAGTDTSSSTVEWAIAELIRHPDILAQVKQ 339

Query: 676  ELDSVVGRDRLVSELDLANLPLMQAIIKETFRLHPSTPLSLPHVGSEPCEVGGFHIPQGS 855
            ELDSVVGRDRLV+ELDLA L  +QA++KETFRLHPSTPLSLP + +E CE+GG+HIP+GS
Sbjct: 340  ELDSVVGRDRLVTELDLAQLTYLQAVVKETFRLHPSTPLSLPRIAAESCEIGGYHIPKGS 399

Query: 856  TILINIWSISRDPNFWSNPLEFEPSRFLPGGNHADVDLKGSHFEFIPFGAGRRICAGMRL 1035
            T+L+N+W+I+RDP+ W+ PLEF P RFLPGG+ ADVD+KG+ FE IPFGAGRRICAGM L
Sbjct: 400  TVLVNVWAIARDPDVWTKPLEFRPERFLPGGDKADVDVKGNDFELIPFGAGRRICAGMSL 459

Query: 1036 GLRMVPLVVA 1065
            GLRMV L+ A
Sbjct: 460  GLRMVQLLTA 469



 Score = 71.2 bits (173), Expect(2) = e-144
 Identities = 32/48 (66%), Positives = 36/48 (75%)
 Frame = +2

Query: 1187 SLVHGFDWALPDGLTPETLNMDEDFGLTLQRAVPLVARPIPRLSPDAY 1330
            +L+H FDW L DGL PE LNMDE +GLTLQRA PL+  P PRLSP  Y
Sbjct: 470  TLIHAFDWDLADGLVPEKLNMDEAYGLTLQRADPLMVHPRPRLSPKVY 517


>gb|ADE80941.1| flavonoid 3'-hydroxylase [Epimedium sagittatum]
          Length = 514

 Score =  468 bits (1203), Expect(2) = e-143
 Identities = 225/364 (61%), Positives = 283/364 (77%), Gaps = 9/364 (2%)
 Frame = +1

Query: 1    PPNSGAEHVAYNYQDLVFAPYGPQWRMFRRLCSLHLFSTKVLDELRHVREDEVTLLVTGL 180
            PPNSGA+H+AYNYQDLVFAPYGP+WRM R++CS+HLFS K LD+ RH+R++EV +L+  L
Sbjct: 100  PPNSGAKHIAYNYQDLVFAPYGPKWRMLRKICSVHLFSAKALDDFRHIRQEEVLVLINAL 159

Query: 181  RRAEKVDVEVGKEINICVTNALSKAMLGRKVFDDGDNGE----FKDLVVELMKLSGSFNV 348
             +A K   ++ K +N+C TN L + MLGR+VF DG   E    FK +VVE+M L+G FN+
Sbjct: 160  FQAGKAPTDLAKLLNVCTTNVLGRVMLGRRVFGDGSGDEKSEEFKQMVVEMMVLAGVFNI 219

Query: 349  GDFVPWLKPLDVHGVVSRMKKIHKWYDDFLNKIIEDHNKEGGRG-----DLLSVLLRLKE 513
            GDFVP L+ LD+ GV S+MKK+H  +D+FLNKI+E+H +  G       DLLSVL+ LK+
Sbjct: 220  GDFVPSLEWLDLQGVASKMKKLHNRFDEFLNKILEEHKESNGEDGHVHTDLLSVLIGLKD 279

Query: 514  DHDNNDEEIKLTDTNIKALLLDLFQAGTDTTSSTVEWGLAELLRHPDILTAAQNELDSVV 693
            D +   E  KLTDTNIKALLLDLF AGTDT+SSTVEW +AEL+R+P +L  AQ EL+ VV
Sbjct: 280  DAEG--EGGKLTDTNIKALLLDLFTAGTDTSSSTVEWAIAELIRNPKLLAQAQEELNQVV 337

Query: 694  GRDRLVSELDLANLPLMQAIIKETFRLHPSTPLSLPHVGSEPCEVGGFHIPQGSTILINI 873
            GRDRLVSE DL  L   QAIIKETFRLHPSTPLSLP + SE CE+ G+HIP+ ST+L+N+
Sbjct: 338  GRDRLVSESDLGQLTFFQAIIKETFRLHPSTPLSLPRMASESCEIDGYHIPKNSTLLVNV 397

Query: 874  WSISRDPNFWSNPLEFEPSRFLPGGNHADVDLKGSHFEFIPFGAGRRICAGMRLGLRMVP 1053
            W+I+RDP+ WS PLEF+P RFLPGG +A +D+KG+ FE IPFGAGRRICAGM +G+RMV 
Sbjct: 398  WAIARDPDVWSEPLEFKPDRFLPGGKNAHMDVKGTDFEVIPFGAGRRICAGMSMGMRMVQ 457

Query: 1054 LVVA 1065
             V A
Sbjct: 458  YVTA 461



 Score = 69.3 bits (168), Expect(2) = e-143
 Identities = 30/48 (62%), Positives = 36/48 (75%)
 Frame = +2

Query: 1187 SLVHGFDWALPDGLTPETLNMDEDFGLTLQRAVPLVARPIPRLSPDAY 1330
            +LVHGFDW +P+G   E LNM+E +GLTLQRA PLV  P PRL+P  Y
Sbjct: 462  TLVHGFDWEMPEGQMVEKLNMEESYGLTLQRAAPLVVHPRPRLAPHVY 509


>gb|AAG49298.1|AF313488_1 putative flavonoid 3'-hydroxylase [Callistephus chinensis]
          Length = 518

 Score =  472 bits (1214), Expect(2) = e-143
 Identities = 227/369 (61%), Positives = 290/369 (78%), Gaps = 14/369 (3%)
 Frame = +1

Query: 1    PPNSGAEHVAYNYQDLVFAPYGPQWRMFRRLCSLHLFSTKVLDELRHVREDEVTLLVTGL 180
            PPNSGA+H+AYNYQDLVFAPYGP+WRM R++CS+HLFSTK LD+ RHVRE+EV +L   L
Sbjct: 99   PPNSGAKHIAYNYQDLVFAPYGPRWRMLRKICSVHLFSTKALDDFRHVREEEVAILTRVL 158

Query: 181  RRAEKVDVEVGKEINICVTNALSKAMLGRKVFDDGDNG--------EFKDLVVELMKLSG 336
              A +  V++G+ +N+C TNAL++ MLGR+VF DG  G        EFKD+VVELM+L+G
Sbjct: 159  VHAGESAVKLGQLLNVCTTNALARVMLGRRVFADGSEGRGVDPKADEFKDMVVELMELAG 218

Query: 337  SFNVGDFVPWLKPLDVHGVVSRMKKIHKWYDDFLNKIIEDHNKEGG-----RGDLLSVLL 501
             FN+GDF+P L  LD+ G+  +MKK+H  +D FLN I++DH  E G       DLL+ L+
Sbjct: 219  EFNIGDFIPPLDCLDLQGITKKMKKLHARFDKFLNIILDDHKIEKGAAGRRHSDLLTTLI 278

Query: 502  RLKE-DHDNNDEEIKLTDTNIKALLLDLFQAGTDTTSSTVEWGLAELLRHPDILTAAQNE 678
             LK+ D  ++DEE KL+D  IKALLL+LF AGTDT+SSTVEW +AEL+RHP++L  A+ E
Sbjct: 279  SLKDVDAADDDEEGKLSDIEIKALLLNLFAAGTDTSSSTVEWAVAELIRHPELLKQAREE 338

Query: 679  LDSVVGRDRLVSELDLANLPLMQAIIKETFRLHPSTPLSLPHVGSEPCEVGGFHIPQGST 858
            +D VVGRDRLV+ELDL+ L  +QAI+KETFRLHPSTPLSLP + SE CEV G++IP+GST
Sbjct: 339  MDIVVGRDRLVTELDLSRLTFLQAIVKETFRLHPSTPLSLPRMASESCEVDGYYIPKGST 398

Query: 859  ILINIWSISRDPNFWSNPLEFEPSRFLPGGNHADVDLKGSHFEFIPFGAGRRICAGMRLG 1038
            +L+N+W+I+RDP  W+NPLEF PSRFLPGG   D D+KG+ FE IPFGAGRRICAGM LG
Sbjct: 399  LLVNVWAIARDPKMWTNPLEFRPSRFLPGGEKPDADIKGNDFEVIPFGAGRRICAGMSLG 458

Query: 1039 LRMVPLVVA 1065
            +RMV L++A
Sbjct: 459  MRMVQLLIA 467



 Score = 64.7 bits (156), Expect(2) = e-143
 Identities = 30/48 (62%), Positives = 35/48 (72%)
 Frame = +2

Query: 1187 SLVHGFDWALPDGLTPETLNMDEDFGLTLQRAVPLVARPIPRLSPDAY 1330
            +LV  FDW L +GL PE LNM+E +GLTLQRA PL+  P PRLSP  Y
Sbjct: 468  TLVQTFDWELANGLDPEKLNMEEAYGLTLQRAEPLMVHPRPRLSPHVY 515


>gb|AGC29948.1| CYP75B65 [Sinopodophyllum hexandrum]
          Length = 510

 Score =  459 bits (1181), Expect(2) = e-142
 Identities = 227/365 (62%), Positives = 285/365 (78%), Gaps = 10/365 (2%)
 Frame = +1

Query: 1    PPNSGAEHVAYNYQDLVFAPYGPQWRMFRRLCSLHLFSTKVLDELRHVREDEVTLLVTGL 180
            PPNSGA+H+AYNYQDLVFAPYGP+WRM R++CS+HLFS K LD+ RH+R++EV +L   L
Sbjct: 100  PPNSGAKHIAYNYQDLVFAPYGPKWRMLRKICSVHLFSAKALDDFRHIRQEEVMVLTKVL 159

Query: 181  RRAEKVDVEVGKEINICVTNALSKAMLGRKVFDDGD----NGEFKDLVVELMKLSGSFNV 348
              A K  V++ K +N+C TNAL + MLGR+VF DG+    + EFK++VVE+M L+G FN+
Sbjct: 160  LGAGKAPVDLAKLLNVCTTNALGRVMLGRRVFGDGNGDEKSDEFKNMVVEMMVLAGIFNI 219

Query: 349  GDFVPWLKPLDVHGVVSRMKKIHKWYDDFLNKIIEDH----NKEGG--RGDLLSVLLRLK 510
            GDFVP L+ LD+  V SRMKK+HK +D FL+KI+E+H    N   G    DLLSVLL LK
Sbjct: 220  GDFVPSLEWLDLQRVASRMKKLHKRFDAFLSKILEEHRESENSNSGVKHTDLLSVLLGLK 279

Query: 511  EDHDNNDEEIKLTDTNIKALLLDLFQAGTDTTSSTVEWGLAELLRHPDILTAAQNELDSV 690
            +D D   E  KLTDTNIKALLLDLF AGTDT+SSTVEW ++EL+R+P +L  AQ ELD V
Sbjct: 280  DDVDG--EGGKLTDTNIKALLLDLFTAGTDTSSSTVEWAISELVRNPKLLAQAQEELDRV 337

Query: 691  VGRDRLVSELDLANLPLMQAIIKETFRLHPSTPLSLPHVGSEPCEVGGFHIPQGSTILIN 870
            VGRDRLVSE DL+ L   QAIIKETFRLHPSTPLSLP + +E CE+ GF+IP+ ST+L+N
Sbjct: 338  VGRDRLVSESDLSQLTFFQAIIKETFRLHPSTPLSLPRMATESCEINGFYIPKDSTLLVN 397

Query: 871  IWSISRDPNFWSNPLEFEPSRFLPGGNHADVDLKGSHFEFIPFGAGRRICAGMRLGLRMV 1050
            +W+I+RDP+ W  PLEF+P RF+PGG +A +D+KG+ FE IPFGAGRRICAGM +G+RMV
Sbjct: 398  VWAIARDPSVWPEPLEFKPERFVPGGRNAHMDVKGNDFEVIPFGAGRRICAGMSMGIRMV 457

Query: 1051 PLVVA 1065
              V A
Sbjct: 458  TFVAA 462



 Score = 75.5 bits (184), Expect(2) = e-142
 Identities = 34/48 (70%), Positives = 39/48 (81%)
 Frame = +2

Query: 1187 SLVHGFDWALPDGLTPETLNMDEDFGLTLQRAVPLVARPIPRLSPDAY 1330
            +LVHGF+W LP+G  PE LNMDE +GLTLQRAVPLV  P PRL+P AY
Sbjct: 463  TLVHGFNWELPEGQMPEKLNMDEAYGLTLQRAVPLVVHPQPRLAPHAY 510


>gb|ADM26615.1| flavonoid 3'-hydroxylase [Cynara cardunculus var. scolymus]
          Length = 512

 Score =  464 bits (1194), Expect(2) = e-142
 Identities = 223/365 (61%), Positives = 282/365 (77%), Gaps = 10/365 (2%)
 Frame = +1

Query: 1    PPNSGAEHVAYNYQDLVFAPYGPQWRMFRRLCSLHLFSTKVLDELRHVREDEVTLLVTGL 180
            PPNSGA+H+AY+YQDLVFAPYGP+WRM R++CS+HLFS K LD+ RHVRE+EV +L   L
Sbjct: 99   PPNSGAKHIAYDYQDLVFAPYGPKWRMLRKICSVHLFSNKALDDFRHVREEEVAILARAL 158

Query: 181  RRAEKVDVEVGKEINICVTNALSKAMLGRKVFDDGDNG------EFKDLVVELMKLSGSF 342
              A +  V +G+ +N+C TNAL++ MLGR+VF DG  G      EFKD+VVELM L+G F
Sbjct: 159  AGAGRSTVALGQLLNVCTTNALARVMLGRRVFGDGSGGVDPKADEFKDMVVELMVLAGEF 218

Query: 343  NVGDFVPWLKPLDVHGVVSRMKKIHKWYDDFLNKIIEDHNKEG----GRGDLLSVLLRLK 510
            N+GDF+P L  LD+  V  +MKK+H  +D FLN ++EDH   G    G  DLLS L+ LK
Sbjct: 219  NIGDFIPALDWLDLQSVTKKMKKLHLRFDSFLNTVLEDHKNGGDVVSGNVDLLSTLISLK 278

Query: 511  EDHDNNDEEIKLTDTNIKALLLDLFQAGTDTTSSTVEWGLAELLRHPDILTAAQNELDSV 690
            +D D   E  KL+D  IKALLL+LF AGTDT+SSTVEW +AEL+RHP ++  AQ E+D V
Sbjct: 279  DDADG--EGGKLSDIEIKALLLNLFAAGTDTSSSTVEWAMAELIRHPQLMKQAQEEMDIV 336

Query: 691  VGRDRLVSELDLANLPLMQAIIKETFRLHPSTPLSLPHVGSEPCEVGGFHIPQGSTILIN 870
            VGRDRLVSELDL+ L  ++AI+KETFRLHPSTPLSLP + SE CEV G++IP+GST+L+N
Sbjct: 337  VGRDRLVSELDLSRLTFLEAIVKETFRLHPSTPLSLPRIASESCEVNGYYIPKGSTLLVN 396

Query: 871  IWSISRDPNFWSNPLEFEPSRFLPGGNHADVDLKGSHFEFIPFGAGRRICAGMRLGLRMV 1050
            +W+I+RDP  W++PLEF PSRFLPGG   + ++KG+ FE IPFGAGRRICAGM LGLRMV
Sbjct: 397  VWAIARDPKMWTDPLEFRPSRFLPGGEKPNANVKGNDFEIIPFGAGRRICAGMSLGLRMV 456

Query: 1051 PLVVA 1065
             L++A
Sbjct: 457  QLLIA 461



 Score = 69.3 bits (168), Expect(2) = e-142
 Identities = 31/48 (64%), Positives = 36/48 (75%)
 Frame = +2

Query: 1187 SLVHGFDWALPDGLTPETLNMDEDFGLTLQRAVPLVARPIPRLSPDAY 1330
            SLVH FDW L +GL PE LNM+E +GLTLQRA PL+  P PRL+P  Y
Sbjct: 462  SLVHAFDWELANGLDPEKLNMEEAYGLTLQRAAPLMVHPSPRLAPHLY 509