BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dioscorea21_contig00022928
         (1524 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002510131.1| cytochrome P450, putative [Ricinus communis]...   439   e-120
gb|AGC29952.1| CYP81B57 [Sinopodophyllum hexandrum]                   436   e-120
ref|XP_004169118.1| PREDICTED: isoflavone 2'-hydroxylase-like [C...   431   e-118
ref|XP_004142688.1| PREDICTED: isoflavone 2'-hydroxylase-like [C...   429   e-118
ref|XP_002283222.2| PREDICTED: isoflavone 2'-hydroxylase-like [V...   429   e-117

>ref|XP_002510131.1| cytochrome P450, putative [Ricinus communis]
            gi|223550832|gb|EEF52318.1| cytochrome P450, putative
            [Ricinus communis]
          Length = 509

 Score =  439 bits (1128), Expect = e-120
 Identities = 224/425 (52%), Positives = 304/425 (71%), Gaps = 10/425 (2%)
 Frame = -3

Query: 1270 ECFSSQNDIVFANRPHLFAGKHLGYNYTTIAWASYGPHWRNLRRLTTLELFSTLRLNSFS 1091
            ECF+ +NDI+ ANRP L AGKHLGY+YTT+ WASYG HWRNLRR+ ++EL S+ R+ +FS
Sbjct: 85   ECFT-KNDIILANRPKLLAGKHLGYDYTTLVWASYGNHWRNLRRIASIELLSSNRIQTFS 143

Query: 1090 SIRRDEVLSLVKSLVKDNKK-----VVLKPKLFELTLNIMMRMIAGKKYFGXXXXXXXXX 926
            ++R +EV SL + L + +       V +K  LFELTLN++MRMIAGK+Y+G         
Sbjct: 144  NVRVEEVRSLARRLFRGSMDGEFMTVDMKSMLFELTLNVLMRMIAGKRYYGENTAELDDA 203

Query: 925  XXXERFRDIVEETFRVSGSSNLCDFMPFLRLFGYGRRMEKRIVRLQEKRDAFLQELIDKH 746
                +F++IV ETF++SG+SN+ DF+P L+  G    +EKR+  LQ KRD F+QEL+++H
Sbjct: 204  K---KFKEIVTETFQLSGASNIGDFVPALKWVGL-TNIEKRLEILQRKRDRFMQELVEEH 259

Query: 745  RDKTRVNPNVNDHFEGK--KSXXXXXXXXXXXDPEYYTDDIIKGVVVMMLTAGTDTSAVT 572
            +       N +D   GK  K+           +PEYYTD+II+G++++MLTAG+DTSA T
Sbjct: 260  K-----RANSDDSASGKRCKTMIDVLLDLQKDEPEYYTDEIIRGMMLVMLTAGSDTSAGT 314

Query: 571  IEWAMSLLLNHPEVLSKVEEEIMINIGKERFVTELDLANLSFLQCVIYETLRLKPPAPLI 392
            +EWA++LLLN+PE L K  EEI  N+G+ + + E D+ANL +LQ +I ET R++P APL+
Sbjct: 315  LEWALTLLLNNPEALLKAREEIDTNVGQSKLIEESDIANLPYLQGIINETFRMQPAAPLL 374

Query: 391  PAHESSEDCKISGFHVSRGTMLLVNAWAMQRDPELWVEPNKFQPERWL---GKGREERQK 221
            PAHESSE+C + GF + RGTMLLVN +A+Q DP+LW EP KF+PER+L   GKG      
Sbjct: 375  PAHESSEECILGGFKIPRGTMLLVNMFAIQNDPKLWEEPTKFKPERFLSTEGKGEGLGYM 434

Query: 220  MLPFGIGRRSCPGEGLAMKVVGLTLAALIQCFEWGRVDDELVDLHEGPGLTMPKACPLVA 41
            +LPFG GRR CPGEGLA++ +GL L  LIQCFEW R+ +E+VD+ EG GL+MPKA PLVA
Sbjct: 435  LLPFGAGRRRCPGEGLAIRNIGLGLGTLIQCFEWERIGEEMVDMVEGSGLSMPKAHPLVA 494

Query: 40   LCKPR 26
             C+PR
Sbjct: 495  KCRPR 499


>gb|AGC29952.1| CYP81B57 [Sinopodophyllum hexandrum]
          Length = 507

 Score =  436 bits (1121), Expect = e-120
 Identities = 227/420 (54%), Positives = 295/420 (70%), Gaps = 4/420 (0%)
 Frame = -3

Query: 1270 ECFSSQNDIVFANRPHLFAGKHLGYNYTTIAWASYGPHWRNLRRLTTLELFSTLRLNSFS 1091
            EC + +NDI FANRP L  GKH G NYTT+AWASYG +WRNLRR++ LE+FS  R+  FS
Sbjct: 85   ECLT-KNDIGFANRPRLMIGKHSGNNYTTLAWASYGQNWRNLRRISALEMFSPNRIQMFS 143

Query: 1090 SIRRDEVLSLVKSLVK--DNKKVVLKPKLFELTLNIMMRMIAGKKYFGXXXXXXXXXXXX 917
             IR DEV S+V+ LV   +  KV +K   FELTLNIMMRMIAGK+Y+G            
Sbjct: 144  EIRTDEVKSMVRRLVAGYEYHKVEMKSVFFELTLNIMMRMIAGKRYYGEHVADLEQAR-- 201

Query: 916  ERFRDIVEETFRVSGSSNLCDFMPFLRLFGYGRRMEKRIVRLQEKRDAFLQELIDKHRDK 737
             +F++IVEETF +SG+SN+ DF+P LR  GY + +EKR++RL+ KRD FLQELI + R K
Sbjct: 202  -KFKEIVEETFELSGASNVGDFLPLLRWIGY-KGIEKRLIRLKNKRDEFLQELIQERR-K 258

Query: 736  TRVNPNVNDHFEGKKSXXXXXXXXXXXDPEYYTDDIIKGVVVMMLTAGTDTSAVTIEWAM 557
             RVN       E KK+           +PEYYTD++I+G++ ++  AGTDTSA T+EWAM
Sbjct: 259  LRVNSKSFPSEEKKKTLIDVLLSLQETEPEYYTDEMIRGLIWILFAAGTDTSAGTMEWAM 318

Query: 556  SLLLNHPEVLSKVEEEIMINIGKERFVTELDLANLSFLQCVIYETLRLKPPAPLIPAHES 377
            SLLLN+P+++ K + EI + I   R + E DL  L +L C+I ETLR+ P  PL+  HES
Sbjct: 319  SLLLNNPDLIKKAQSEIDLQIEPGRPIDESDLNKLPYLHCIINETLRMYPAGPLLIPHES 378

Query: 376  SEDCKISGFHVSRGTMLLVNAWAMQRDPELWVEPNKFQPERWLG--KGREERQKMLPFGI 203
            SEDC+I G+++  GTMLLVN WA+Q DP LW EP KF+PER+ G   G  +  K++PFG 
Sbjct: 379  SEDCEIGGYNIPCGTMLLVNLWAIQNDPNLWKEPRKFKPERFEGYQGGVRDGFKLMPFGS 438

Query: 202  GRRSCPGEGLAMKVVGLTLAALIQCFEWGRVDDELVDLHEGPGLTMPKACPLVALCKPRS 23
            GRR CPGEGLA +VVGLTL +L+QCF+W RV +E+V++ EG GLT+PK  PL A C+PRS
Sbjct: 439  GRRGCPGEGLAXRVVGLTLXSLLQCFDWHRVGEEMVEMSEGTGLTLPKLHPLEAHCRPRS 498


>ref|XP_004169118.1| PREDICTED: isoflavone 2'-hydroxylase-like [Cucumis sativus]
          Length = 506

 Score =  431 bits (1107), Expect = e-118
 Identities = 220/423 (52%), Positives = 292/423 (69%), Gaps = 4/423 (0%)
 Frame = -3

Query: 1270 ECFSSQNDIVFANRPHLFAGKHLGYNYTTIAWASYGPHWRNLRRLTTLELFSTLRLNSFS 1091
            +CFS QNDIVFANRP L AGK+LGY+++ + WASYG HWRNLRR+++L L S+  L S S
Sbjct: 85   QCFS-QNDIVFANRPRLLAGKYLGYDFSVVVWASYGDHWRNLRRISSLHLLSSSNLQSLS 143

Query: 1090 SIRRDEVLSLVKSLVKDNKKVV-LKPKLFELTLNIMMRMIAGKKYFGXXXXXXXXXXXXE 914
            S+R DEV SL+  L K+  +VV ++  LFE  LN+MMRMI GK+YF              
Sbjct: 144  SVRADEVHSLILRLRKNPNQVVNVRTLLFEFMLNVMMRMIGGKRYFDDNRTHTEESL--- 200

Query: 913  RFRDIVEETFRVSGSSNLCDFMPFLRLFGYGRRMEKRIVRLQEKRDAFLQELIDKHRDKT 734
             F++IV ETF+++G++NL D++P L+  G  R++EKR + L++KRD  +Q LI++HR + 
Sbjct: 201  NFQEIVTETFKLAGANNLVDYLPILKWTGISRKIEKRYINLRKKRDKLIQNLIEEHRKEK 260

Query: 733  RVNPNVNDHFEGKKSXXXXXXXXXXXDPEYYTDDIIKGVVVMMLTAGTDTSAVTIEWAMS 554
            +   + N     K +           DP+YYTD+II G +++ML+AGTDTS  T+EWAMS
Sbjct: 261  QKAMSKNKPPLKKTTMIEVMLSLQESDPDYYTDEIIIGQMMVMLSAGTDTSVGTMEWAMS 320

Query: 553  LLLNHPEVLSKVEEEIMINIGKERFVTELDLANLSFLQCVIYETLRLKPPAPLIPAHESS 374
            LLLNHP+VL K +EEI   IGK+R + E DL  L +LQCVI ET+R+ P  PL+  HESS
Sbjct: 321  LLLNHPDVLGKAKEEIDEIIGKKRHIEESDLEKLPYLQCVIKETMRMYPVGPLLVPHESS 380

Query: 373  EDCKISGFHVSRGTMLLVNAWAMQRDPELWVEPNKFQPERWLGKGREE---RQKMLPFGI 203
             DC + G+H+  GTML+VNAWA+  D  LW E   F+PER+LG G E      K + FG 
Sbjct: 381  ADCTVGGYHIPGGTMLMVNAWAIHNDAGLWEEAAVFKPERFLGAGAEGDGIGLKYMVFGA 440

Query: 202  GRRSCPGEGLAMKVVGLTLAALIQCFEWGRVDDELVDLHEGPGLTMPKACPLVALCKPRS 23
            GRR CPGEGLAM+VVGL L +LIQCFEW R+ +E+VD+ EG GLTMPKACPL A C+PR 
Sbjct: 441  GRRGCPGEGLAMRVVGLVLGSLIQCFEWERIGEEMVDMGEGTGLTMPKACPLQAKCRPRP 500

Query: 22   FVY 14
             ++
Sbjct: 501  ILH 503


>ref|XP_004142688.1| PREDICTED: isoflavone 2'-hydroxylase-like [Cucumis sativus]
          Length = 506

 Score =  429 bits (1104), Expect = e-118
 Identities = 219/423 (51%), Positives = 292/423 (69%), Gaps = 4/423 (0%)
 Frame = -3

Query: 1270 ECFSSQNDIVFANRPHLFAGKHLGYNYTTIAWASYGPHWRNLRRLTTLELFSTLRLNSFS 1091
            +CFS QNDIVFANRP L AGK+LGY+++ + WASYG HWRNLRR+++L L S+  L S S
Sbjct: 85   QCFS-QNDIVFANRPRLLAGKYLGYDFSVVVWASYGDHWRNLRRISSLHLLSSSNLQSLS 143

Query: 1090 SIRRDEVLSLVKSLVKDNKKVV-LKPKLFELTLNIMMRMIAGKKYFGXXXXXXXXXXXXE 914
            S+R DEV SL+  L K+  +VV ++  LFE  LN+MMRMI GK+YF              
Sbjct: 144  SVRADEVHSLILRLRKNPNQVVNVRTLLFEFMLNVMMRMIGGKRYFDDNRTHTEESL--- 200

Query: 913  RFRDIVEETFRVSGSSNLCDFMPFLRLFGYGRRMEKRIVRLQEKRDAFLQELIDKHRDKT 734
             F++IV ETF+++G++NL D++P L+  G  R++EKR + L++KRD  +Q LI++HR + 
Sbjct: 201  NFQEIVTETFKLAGANNLVDYLPILKWTGISRKIEKRYINLRKKRDKLIQNLIEEHRKEK 260

Query: 733  RVNPNVNDHFEGKKSXXXXXXXXXXXDPEYYTDDIIKGVVVMMLTAGTDTSAVTIEWAMS 554
            +   + N     K +           DP+YYTD+II G +++ML+AGTDTS  T+EWAMS
Sbjct: 261  QKAMSKNKPPLKKTTMIEMMLSLQESDPDYYTDEIIIGQMMVMLSAGTDTSVGTMEWAMS 320

Query: 553  LLLNHPEVLSKVEEEIMINIGKERFVTELDLANLSFLQCVIYETLRLKPPAPLIPAHESS 374
            LLLNHP+VL K +EE+   IGK+R + E DL  L +LQCVI ET+R+ P  PL+  HESS
Sbjct: 321  LLLNHPDVLGKAKEEMDEIIGKKRHIEESDLEKLPYLQCVIKETMRMYPVGPLLVPHESS 380

Query: 373  EDCKISGFHVSRGTMLLVNAWAMQRDPELWVEPNKFQPERWLGKGREE---RQKMLPFGI 203
             DC + G+H+  GTML+VNAWA+  D  LW E   F+PER+LG G E      K + FG 
Sbjct: 381  ADCTVGGYHIPGGTMLMVNAWAIHNDAGLWEEAAVFKPERFLGAGAEGDGIGLKYMVFGA 440

Query: 202  GRRSCPGEGLAMKVVGLTLAALIQCFEWGRVDDELVDLHEGPGLTMPKACPLVALCKPRS 23
            GRR CPGEGLAM+VVGL L +LIQCFEW R+ +E+VD+ EG GLTMPKACPL A C+PR 
Sbjct: 441  GRRGCPGEGLAMRVVGLVLGSLIQCFEWERIGEEMVDMGEGTGLTMPKACPLQAKCRPRP 500

Query: 22   FVY 14
             ++
Sbjct: 501  ILH 503


>ref|XP_002283222.2| PREDICTED: isoflavone 2'-hydroxylase-like [Vitis vinifera]
          Length = 503

 Score =  429 bits (1102), Expect = e-117
 Identities = 227/422 (53%), Positives = 292/422 (69%), Gaps = 7/422 (1%)
 Frame = -3

Query: 1270 ECFSSQNDIVFANRPHLFAGKHLGYNYTTIAWASYGPHWRNLRRLTTLELFSTLRLNSFS 1091
            ECF+ +NDIVFANRP L AGKHLGYNYTT+ WA YG HWRNLRR+ +LE+ S+ RL  F 
Sbjct: 84   ECFT-KNDIVFANRPRLLAGKHLGYNYTTLTWAPYGQHWRNLRRIASLEILSSNRLQMFY 142

Query: 1090 SIRRDEVLSLVKSLVKDNKK-----VVLKPKLFELTLNIMMRMIAGKKYFGXXXXXXXXX 926
             IR DEV +L+  L + + +     V +K   FELTLN MMRMI+GK+Y+G         
Sbjct: 143  DIRIDEVRALLCQLFRASSEGQFSAVDMKSMFFELTLNNMMRMISGKRYYGDNVTELEET 202

Query: 925  XXXERFRDIVEETFRVSGSSNLCDFMPFLRLFGYGRRMEKRIVRLQEKRDAFLQELIDKH 746
                +FR+IV ETF +SG++N+ DF+PF +  G    +EK++V LQ KRD F+Q LI++H
Sbjct: 203  R---KFREIVAETFELSGATNIVDFVPFSKWIGLNG-IEKKLVILQGKRDGFMQNLIEEH 258

Query: 745  RDKTRVNPNVNDHFEGK-KSXXXXXXXXXXXDPEYYTDDIIKGVVVMMLTAGTDTSAVTI 569
            R        +    EG+ K+           +PE YTDDII+G++ +ML+AGTDTSA T+
Sbjct: 259  R-------RMRSPCEGRSKTMLDVLLSLQETEPECYTDDIIRGMMQVMLSAGTDTSAGTM 311

Query: 568  EWAMSLLLNHPEVLSKVEEEIMINIGKERFVTELDLANLSFLQCVIYETLRLKPPAPLIP 389
            EWAMSLLLN+PE L K + EI  ++GK R + ELD+A L +L+ +I ETLR+ P APL+ 
Sbjct: 312  EWAMSLLLNNPEALEKAQAEIDSHLGKSRLIDELDIAELPYLRGIIKETLRMYPAAPLLV 371

Query: 388  AHESSEDCKISGFHVSRGTMLLVNAWAMQRDPELWVEPNKFQPERWLG-KGREERQKMLP 212
             HESSE+C + GF V  GTMLLVN WA+Q DP LW EP+KF+PER+ G +G+       P
Sbjct: 372  PHESSEECTVGGFRVPSGTMLLVNMWAIQNDPMLWAEPSKFKPERFQGPEGQRNGFMFSP 431

Query: 211  FGIGRRSCPGEGLAMKVVGLTLAALIQCFEWGRVDDELVDLHEGPGLTMPKACPLVALCK 32
            FG GRR CPGEGLAM+VVGL L +LIQ FEW RVD+E+VD+ EG GLTMPKA  LVA C+
Sbjct: 432  FGAGRRGCPGEGLAMRVVGLALGSLIQFFEWERVDEEMVDMSEGTGLTMPKAQSLVAKCR 491

Query: 31   PR 26
            PR
Sbjct: 492  PR 493