BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dioscorea21_contig00022537
         (1117 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ABA94379.2| RWD domain containing protein, expressed [Oryza s...   221   7e-80
ref|NP_001148707.1| LOC100282323 [Zea mays] gi|195621552|gb|ACG3...   223   8e-80
ref|XP_002449721.1| hypothetical protein SORBIDRAFT_05g022090 [S...   213   1e-77
ref|NP_001241545.1| uncharacterized protein LOC100791789 [Glycin...   210   1e-76
ref|XP_003577450.1| PREDICTED: E3 ubiquitin-protein ligase RNF25...   213   3e-75

>gb|ABA94379.2| RWD domain containing protein, expressed [Oryza sativa Japonica
           Group]
          Length = 379

 Score =  221 bits (564), Expect(2) = 7e-80
 Identities = 94/149 (63%), Positives = 126/149 (84%)
 Frame = +3

Query: 102 YGDDCEIIREFPPHLHVHITPSTAENSSQQFVEVVLGILCDAQYPQKPPHLHIVDSKGLD 281
           YGDDC ++R FPPHL VH+ P TA++SSQQFVE+ LGI   +QYP+ PPH++ V+SKGLD
Sbjct: 40  YGDDCRVVRGFPPHLVVHVRPRTADDSSQQFVELFLGIKASSQYPKAPPHVYAVESKGLD 99

Query: 282 ENREKHLITSIENKSQELSSCSMIIELCEEAVELLTGMNHPEGNCPFCLHPLLVEDQSGN 461
           ENR+ +LI+SI+NK++ELS   M++ LCEEAVE+L+ MNHP G+CP CL+P LV++  G+
Sbjct: 100 ENRQVYLISSIQNKARELSCYPMLVTLCEEAVEMLSNMNHPAGDCPLCLYP-LVKENDGS 158

Query: 462 SPPFMKLMSCYHCFHSECIIQWWQWLQDD 548
           + PFMKLMSCYHCFHS+CI++WW+WLQ+D
Sbjct: 159 ALPFMKLMSCYHCFHSDCIMRWWEWLQND 187



 Score =  103 bits (257), Expect(2) = 7e-80
 Identities = 59/149 (39%), Positives = 91/149 (61%), Gaps = 4/149 (2%)
 Frame = +1

Query: 655  KDIEHILDYAGTNSLQ-ENAAEEMDADERKLLESESEKNRRQKFESLLQLQQENSGLIEP 831
            KDIEH+ D  G N+ Q  + + ++D DE+++L SESEK R++KF SL  LQQE +GLIEP
Sbjct: 224  KDIEHVRDLLGANTSQLASLSIDLDEDEKEVLHSESEKIRQEKFASLFNLQQERNGLIEP 283

Query: 832  RKEIVVLPGMYLP-APVVPASTAIDSAVEQQLDSASTASEAPDFNIPSNKATTSKHRDMN 1008
            +K++ + PGMY+P  P  P ++  D+  +   D+ ++ S     N  ++   T+KH   +
Sbjct: 284  KKDLSIQPGMYIPRPPSAPTASEGDTVDDSCEDTTTSTSGTEPLNQTNSTGGTTKHNSSS 343

Query: 1009 -TRRKNRYHNSRRQQQNV-YSNRRQWIKK 1089
              RR++R H SRRQ        R+QW +K
Sbjct: 344  GHRRRHRSHASRRQPHGQGQPARQQWQRK 372


>ref|NP_001148707.1| LOC100282323 [Zea mays] gi|195621552|gb|ACG32606.1|
           ubiquitin-protein ligase/ zinc ion binding protein [Zea
           mays] gi|219884689|gb|ACL52719.1| unknown [Zea mays]
           gi|413925312|gb|AFW65244.1| Ubiquitin-protein ligase/
           zinc ion binding protein [Zea mays]
          Length = 358

 Score =  223 bits (567), Expect(2) = 8e-80
 Identities = 92/147 (62%), Positives = 124/147 (84%)
 Frame = +3

Query: 102 YGDDCEIIREFPPHLHVHITPSTAENSSQQFVEVVLGILCDAQYPQKPPHLHIVDSKGLD 281
           YGDD  ++R+ PPHL VH+ P TA+++SQQFVE+ LGI   +QYP++PPH++ V+SKGLD
Sbjct: 25  YGDDVRVLRDLPPHLFVHVRPRTADDTSQQFVELFLGIKASSQYPKEPPHIYAVESKGLD 84

Query: 282 ENREKHLITSIENKSQELSSCSMIIELCEEAVELLTGMNHPEGNCPFCLHPLLVEDQSGN 461
           ENR+ +LITSI+NK+ ELS+C M++ LCEEA E+L+ MNHP G+CP CL+PL+ ED+ G+
Sbjct: 85  ENRQIYLITSIQNKANELSNCPMLVTLCEEAGEILSNMNHPAGDCPLCLYPLMGEDKVGS 144

Query: 462 SPPFMKLMSCYHCFHSECIIQWWQWLQ 542
             PFMKLMSCYHCFHS+CI++WW+WLQ
Sbjct: 145 ELPFMKLMSCYHCFHSDCIMRWWEWLQ 171



 Score =  102 bits (253), Expect(2) = 8e-80
 Identities = 58/148 (39%), Positives = 91/148 (61%), Gaps = 2/148 (1%)
 Frame = +1

Query: 655  KDIEHILDYAGTNSLQ-ENAAEEMDADERKLLESESEKNRRQKFESLLQLQQENSGLIEP 831
            KDIEH+ D  G N+ +  +   ++  DE++LL SE+E+NRR++ ESL+ LQQE +GLIEP
Sbjct: 206  KDIEHVRDLLGDNTSRLASLTVDLGEDEKELLHSEAEQNRRKRIESLVNLQQELNGLIEP 265

Query: 832  RKEIVVLPGMYLPAPVVPASTAIDSAVEQQLDSASTASEAPDFNIPSNKATTSKHRDMNT 1011
            +K++ + PGMY+  P     TA +   +    +A++ SE       SN A+++K ++   
Sbjct: 266  KKDLAIQPGMYVSVPPSTPDTAEEENADPSQGTATSTSETEQRGQASN-ASSNKPKNPGH 324

Query: 1012 RRKNRYHNSRRQQQNV-YSNRRQWIKKE 1092
            RR+NR + SRRQ        R+QW +KE
Sbjct: 325  RRRNRANASRRQPHGQGQPARQQWQRKE 352


>ref|XP_002449721.1| hypothetical protein SORBIDRAFT_05g022090 [Sorghum bicolor]
           gi|241935564|gb|EES08709.1| hypothetical protein
           SORBIDRAFT_05g022090 [Sorghum bicolor]
          Length = 353

 Score =  213 bits (541), Expect(2) = 1e-77
 Identities = 90/147 (61%), Positives = 122/147 (82%)
 Frame = +3

Query: 102 YGDDCEIIREFPPHLHVHITPSTAENSSQQFVEVVLGILCDAQYPQKPPHLHIVDSKGLD 281
           YGDD  ++R+ PPHL V + P TA++SSQQFVE+ LGI   +QYP++PPH++ V+SKGLD
Sbjct: 23  YGDDVRVLRDLPPHLVVDVRPRTADDSSQQFVELFLGIKASSQYPKEPPHIYAVESKGLD 82

Query: 282 ENREKHLITSIENKSQELSSCSMIIELCEEAVELLTGMNHPEGNCPFCLHPLLVEDQSGN 461
           ENR+ +LITSI++K++ELS+  M + LCEEA E+L+ MNHP G+CP CL+PL+ ED+ G+
Sbjct: 83  ENRQIYLITSIQSKAKELSNYPMFVTLCEEAGEILSNMNHPAGDCPLCLYPLVGEDKVGS 142

Query: 462 SPPFMKLMSCYHCFHSECIIQWWQWLQ 542
             PFMKLMSCYHCFHS+CI++WW+WLQ
Sbjct: 143 ELPFMKLMSCYHCFHSDCIMRWWEWLQ 169



 Score =  104 bits (260), Expect(2) = 1e-77
 Identities = 57/148 (38%), Positives = 93/148 (62%), Gaps = 2/148 (1%)
 Frame = +1

Query: 655  KDIEHILDYAGTNSLQ-ENAAEEMDADERKLLESESEKNRRQKFESLLQLQQENSGLIEP 831
            KDIEH+ D  G N+ +  +   ++  DE +LL SE+E+NRR++ ES++ LQQE +GLIEP
Sbjct: 204  KDIEHVRDLLGDNTSRLVSLTGDLGEDETELLHSEAEQNRRKRIESIVNLQQERNGLIEP 263

Query: 832  RKEIVVLPGMYLPAPVVPASTAIDSAVEQQLDSASTASEAPDFNIPSNKATTSKHRDMNT 1011
            +K++ + PGMY+  P     TA +   +   D+A++ SE     + +N  +++K ++   
Sbjct: 264  KKDLAIQPGMYVSLPPSTPETAEEENADPSKDTATSTSETEHRGL-ANNTSSNKPKNSGH 322

Query: 1012 RRKNRYHNSRRQ-QQNVYSNRRQWIKKE 1092
            RR+NR + SRRQ   +  S R+QW +KE
Sbjct: 323  RRRNRANASRRQPHPHGQSARQQWQRKE 350


>ref|NP_001241545.1| uncharacterized protein LOC100791789 [Glycine max]
           gi|255644864|gb|ACU22932.1| unknown [Glycine max]
          Length = 366

 Score =  210 bits (535), Expect(2) = 1e-76
 Identities = 89/151 (58%), Positives = 116/151 (76%)
 Frame = +3

Query: 102 YGDDCEIIREFPPHLHVHITPSTAENSSQQFVEVVLGILCDAQYPQKPPHLHIVDSKGLD 281
           YG DC ++R FPPH H+ + P TA+ SSQQFVE VL I   +QYP++PP + +VD KGLD
Sbjct: 21  YGTDCVLLRSFPPHFHISLKPRTADVSSQQFVEAVLEIQASSQYPKEPPSVDLVDCKGLD 80

Query: 282 ENREKHLITSIENKSQELSSCSMIIELCEEAVELLTGMNHPEGNCPFCLHPLLVEDQSGN 461
           E R+K+L+  I++K+ ELS C M++ LCEEA E L+ MNHP+G+CP CL PL+ EDQ   
Sbjct: 81  EQRKKYLLNCIQSKAYELSPCLMLVALCEEAAEKLSSMNHPDGDCPLCLFPLVPEDQESG 140

Query: 462 SPPFMKLMSCYHCFHSECIIQWWQWLQDDRK 554
           + PFMKLMSC+HCFHSECII+WW+WLQ+  K
Sbjct: 141 TLPFMKLMSCFHCFHSECIIRWWKWLQNSNK 171



 Score =  103 bits (258), Expect(2) = 1e-76
 Identities = 63/156 (40%), Positives = 97/156 (62%), Gaps = 10/156 (6%)
 Frame = +1

Query: 655  KDIEHILDYAGTNSLQENAAEEMDADERKLLESESEKNRRQKFESLLQLQQENSGLIEPR 834
            KD++H+L   G+ S Q ++  E   +E KLL+SE E NRRQK+E++L LQ+ENSGLIEP+
Sbjct: 209  KDLDHVLALVGSYSSQLSSDNEELNEEEKLLQSEDEINRRQKYEAILNLQKENSGLIEPK 268

Query: 835  KEIVVLPGMYLPAPV-VPASTAIDSAVE-QQLDSASTASE-----APDFNIPSNKATTSK 993
            KE+V+LPGMYLP PV +P+ST    + E QQ D  +   E       + +IPS    +S+
Sbjct: 269  KEMVILPGMYLPQPVAMPSSTPTKESDEPQQKDPPAVVPEKHAGGTSNSSIPS----SSR 324

Query: 994  HRDMNTRR---KNRYHNSRRQQQNVYSNRRQWIKKE 1092
             R+   RR   ++  H+S   +      ++QW++++
Sbjct: 325  QRNFGARRQKARSDNHSSSTARNPRKPAQQQWVRRD 360


>ref|XP_003577450.1| PREDICTED: E3 ubiquitin-protein ligase RNF25-like isoform 3
           [Brachypodium distachyon]
          Length = 367

 Score =  213 bits (541), Expect(2) = 3e-75
 Identities = 90/149 (60%), Positives = 121/149 (81%)
 Frame = +3

Query: 102 YGDDCEIIREFPPHLHVHITPSTAENSSQQFVEVVLGILCDAQYPQKPPHLHIVDSKGLD 281
           YGDDC ++ +FPPHL VH+ P+TA++SSQQFVE+ LGI   +QYP++PP ++ V+SKGLD
Sbjct: 20  YGDDCRVVLDFPPHLVVHVRPNTADDSSQQFVELFLGIKASSQYPKEPPDVYAVESKGLD 79

Query: 282 ENREKHLITSIENKSQELSSCSMIIELCEEAVELLTGMNHPEGNCPFCLHPLLVEDQSGN 461
           E R+ +LI+SI+NK++ELS   M++ LCEEA E+L+ MNHP G+CP CL+PL+ ED+   
Sbjct: 80  ETRQAYLISSIQNKAKELSYYPMLVTLCEEAGEILSNMNHPAGDCPLCLYPLVPEDKDDF 139

Query: 462 SPPFMKLMSCYHCFHSECIIQWWQWLQDD 548
           + PFMKLMSCYHCFHS CI++WW WLQ D
Sbjct: 140 ALPFMKLMSCYHCFHSNCIMRWWGWLQQD 168



 Score = 96.7 bits (239), Expect(2) = 3e-75
 Identities = 56/145 (38%), Positives = 88/145 (60%), Gaps = 3/145 (2%)
 Frame = +1

Query: 655  KDIEHILDY--AGTNSLQENAAEEMDADERKLLESESEKNRRQKFESLLQLQQENSGLIE 828
            KDI+H+ D   A T+ L  +   +++ D+++LL SE+E  RR+KF SL  LQQE +GLIE
Sbjct: 205  KDIKHVHDLLDANTSQLMGSLTIDLEDDDKELLYSEAENKRREKFGSLFNLQQERNGLIE 264

Query: 829  PRKEIVVLPGMYLP-APVVPASTAIDSAVEQQLDSASTASEAPDFNIPSNKATTSKHRDM 1005
            P+K++ + PGMYL   P+ P +T  DS    + ++ ST SE    ++ +N  + +K  + 
Sbjct: 265  PKKDLAIQPGMYLSLPPIEPTTTEADSTNSCEENTTST-SETDPGSMGNNTGSRNKQNNS 323

Query: 1006 NTRRKNRYHNSRRQQQNVYSNRRQW 1080
              RR+NR +  +RQ Q   S  +QW
Sbjct: 324  GHRRRNRAYVPKRQPQG-QSTGQQW 347