BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dioscorea21_contig00021500
         (969 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAK02913.1| predicted protein [Hordeum vulgare subsp. vulgare]    224   2e-68
gb|AEC50089.1| flavonoid 3',5'-hydroxylase [Citrus clementina]        223   2e-68
ref|XP_002314004.1| flavonoid 3',5'-hydroxylase [Populus trichoc...   226   2e-68
gb|AEY80043.1| flavonoid-3',5'-hydroxylase [Aconitum carmichaelii]    233   2e-68
ref|XP_002314003.1| cytochrome P450 flavonoid 3',5'-hydroxylase ...   226   2e-68

>dbj|BAK02913.1| predicted protein [Hordeum vulgare subsp. vulgare]
          Length = 519

 Score =  224 bits (570), Expect(2) = 2e-68
 Identities = 107/207 (51%), Positives = 153/207 (73%), Gaps = 1/207 (0%)
 Frame = -2

Query: 965 LLMRHLLRRKSTLLLPPGPRGYPILGALSLIGPAAHVSLANLAKRYGPIMYMKLGSCDVI 786
           L  R  L R+S   LPPGP G PILGAL  +GPA H  LA LA+++GP+MY+++G+C V+
Sbjct: 24  LAFRRSLNRRSCSRLPPGPPGLPILGALPFVGPAPHAGLAALARKHGPVMYLRMGTCGVV 83

Query: 785 VASTAAAARPFLTTLDLQFSNRPTTIASAKHVSYNGNDFIFANYTPKYKLFRKLSTLHML 606
           VAS+ +AAR FL  LD +F+NRP  +ASA  ++Y   + +FA+Y PK+KL RKL+++H+L
Sbjct: 84  VASSPSAARTFLKALDARFANRP-AVASAADITYGRQNMVFADYGPKWKLMRKLASVHLL 142

Query: 605 GNKAMAGWADVRRDEVRLMVKSMHESSQL-GKLVIVPETLICAMMNIVGRVMLSQRVFDA 429
           G +A+A WA VRRDE   +++ M E++++ G+ V+VPE L+CA+ NIVG++ +S+RVFDA
Sbjct: 143 GPRAVADWAGVRRDEAGRVLRGMKEAAEVTGRAVVVPEVLVCALANIVGQITVSRRVFDA 202

Query: 428 TDPELGSYKELLKKLMTGGGLFNIGDF 348
              E  SYKE++  L+TG GLFNI DF
Sbjct: 203 QGDESSSYKEMIVSLLTGTGLFNISDF 229



 Score = 62.8 bits (151), Expect(2) = 2e-68
 Identities = 27/52 (51%), Positives = 40/52 (76%), Gaps = 2/52 (3%)
 Frame = -3

Query: 349 FVPVIAWMDLQGVQVKLKKGHARMDGMVERMLARHAATVKE--REGCPDFID 200
           FVP ++WMDLQGVQ +L++ H + DG++ ++LA HAAT +E  R+G  DF+D
Sbjct: 229 FVPALSWMDLQGVQARLRRVHRQFDGLITKLLAEHAATAEERARDGRLDFVD 280


>gb|AEC50089.1| flavonoid 3',5'-hydroxylase [Citrus clementina]
          Length = 514

 Score =  223 bits (567), Expect(2) = 2e-68
 Identities = 109/209 (52%), Positives = 154/209 (73%), Gaps = 3/209 (1%)
 Frame = -2

Query: 965 LLMRHLLR---RKSTLLLPPGPRGYPILGALSLIGPAAHVSLANLAKRYGPIMYMKLGSC 795
           L+ R+ +R   RKS+  LPPGP+G+PI+GAL L+G   HV+LA +AK+YGP+MY+K+G+C
Sbjct: 21  LITRYFIRFPIRKSSRPLPPGPKGFPIIGALPLLGAMPHVTLAKMAKKYGPVMYLKMGTC 80

Query: 794 DVIVASTAAAARPFLTTLDLQFSNRPTTIASAKHVSYNGNDFIFANYTPKYKLFRKLSTL 615
           D++VAST  AAR FL TLDL FSNRP   A A H++Y+  D +FA+Y P++KL RK+S L
Sbjct: 81  DMVVASTPDAARAFLKTLDLNFSNRPPN-AGATHLAYDAQDMVFADYGPRWKLLRKISNL 139

Query: 614 HMLGNKAMAGWADVRRDEVRLMVKSMHESSQLGKLVIVPETLICAMMNIVGRVMLSQRVF 435
           HMLG KA+  W++VR  E+  M++++ ESSQ  + V+VPE L  AM N++G+V+LS+RVF
Sbjct: 140 HMLGGKALYDWSNVRNIELGHMLRAICESSQRNEPVVVPEMLTYAMANMIGQVILSRRVF 199

Query: 434 DATDPELGSYKELLKKLMTGGGLFNIGDF 348
                E   +K+++ +LMT  G FNIGDF
Sbjct: 200 VTKGTESNEFKDMVVELMTSAGFFNIGDF 228



 Score = 63.9 bits (154), Expect(2) = 2e-68
 Identities = 26/53 (49%), Positives = 39/53 (73%)
 Frame = -3

Query: 349 FVPVIAWMDLQGVQVKLKKGHARMDGMVERMLARHAATVKEREGCPDFIDLVL 191
           F+P IAW+DLQG++  +KK H R D ++ +M+  H A+  EREG PDF+D+V+
Sbjct: 228 FIPSIAWLDLQGIERGMKKLHNRFDVLLTKMIEEHTASTNEREGKPDFLDIVM 280


>ref|XP_002314004.1| flavonoid 3',5'-hydroxylase [Populus trichocarpa]
           gi|222850412|gb|EEE87959.1| flavonoid 3',5'-hydroxylase
           [Populus trichocarpa]
          Length = 509

 Score =  226 bits (575), Expect(2) = 2e-68
 Identities = 108/206 (52%), Positives = 153/206 (74%)
 Frame = -2

Query: 965 LLMRHLLRRKSTLLLPPGPRGYPILGALSLIGPAAHVSLANLAKRYGPIMYMKLGSCDVI 786
           LL R L+R+ ST  LPPGPRG+PI+GA+ ++G   H +LA +AK+YGP+MY+K+G+C+++
Sbjct: 23  LLTRCLIRKLSTRQLPPGPRGWPIIGAIPVLGAMPHAALAKMAKQYGPVMYLKMGTCNMV 82

Query: 785 VASTAAAARPFLTTLDLQFSNRPTTIASAKHVSYNGNDFIFANYTPKYKLFRKLSTLHML 606
           VAST  AAR FL TLDL FSNRP   A A H++YN  D +FA+Y P++KL RKLS LHML
Sbjct: 83  VASTPDAARAFLKTLDLNFSNRPPN-AGATHLAYNAQDMVFADYGPRWKLLRKLSNLHML 141

Query: 605 GNKAMAGWADVRRDEVRLMVKSMHESSQLGKLVIVPETLICAMMNIVGRVMLSQRVFDAT 426
           G KA+  WA VR  E+  M+++M E+S+ G+ V+VPE L  AM N++G+++LS+RVF   
Sbjct: 142 GGKALEDWAHVRVSELGHMLRAMCEASRKGEPVVVPEMLTYAMANMIGQIILSRRVFVTK 201

Query: 425 DPELGSYKELLKKLMTGGGLFNIGDF 348
             E   +K+++ +LMT  GLFN+GD+
Sbjct: 202 GSESNEFKDMVVELMTSAGLFNVGDY 227



 Score = 60.5 bits (145), Expect(2) = 2e-68
 Identities = 23/53 (43%), Positives = 39/53 (73%)
 Frame = -3

Query: 349 FVPVIAWMDLQGVQVKLKKGHARMDGMVERMLARHAATVKEREGCPDFIDLVL 191
           ++P +AWMDLQG++  +K+ H R D ++ +M+  H AT  ER+G PDF+D+++
Sbjct: 227 YIPSVAWMDLQGIERGMKRLHRRFDVLLTKMMEEHIATAHERKGKPDFLDVLM 279


>gb|AEY80043.1| flavonoid-3',5'-hydroxylase [Aconitum carmichaelii]
          Length = 506

 Score =  233 bits (595), Expect(2) = 2e-68
 Identities = 112/206 (54%), Positives = 156/206 (75%)
 Frame = -2

Query: 965 LLMRHLLRRKSTLLLPPGPRGYPILGALSLIGPAAHVSLANLAKRYGPIMYMKLGSCDVI 786
           L +R L   K    LPPGP+G+P++GAL L+G   HV+LA ++++YGPI+Y+KLGSC ++
Sbjct: 19  LFVRFLCSSKQARKLPPGPKGWPVVGALPLLGSMPHVALAKMSRQYGPIVYLKLGSCGMV 78

Query: 785 VASTAAAARPFLTTLDLQFSNRPTTIASAKHVSYNGNDFIFANYTPKYKLFRKLSTLHML 606
           VAST  +AR FL TLDL FSNRPT  A A H++YN  D +FA+Y P++KL RKL++LHML
Sbjct: 79  VASTPDSARTFLKTLDLNFSNRPTD-AGATHIAYNSQDMVFADYGPRWKLLRKLTSLHML 137

Query: 605 GNKAMAGWADVRRDEVRLMVKSMHESSQLGKLVIVPETLICAMMNIVGRVMLSQRVFDAT 426
           G+KA+  WA VRRDEV  MVK+M+ESS +G++V+VP+ L+ AM N++G+V+LS+RVF   
Sbjct: 138 GSKAIEDWARVRRDEVGYMVKAMYESSCVGEVVVVPDMLVFAMANMLGQVILSRRVFVTK 197

Query: 425 DPELGSYKELLKKLMTGGGLFNIGDF 348
             E   +KE++  LMT  GLFN+GD+
Sbjct: 198 GVESNEFKEMVIDLMTSAGLFNVGDY 223



 Score = 52.8 bits (125), Expect(2) = 2e-68
 Identities = 21/54 (38%), Positives = 37/54 (68%)
 Frame = -3

Query: 349 FVPVIAWMDLQGVQVKLKKGHARMDGMVERMLARHAATVKEREGCPDFIDLVLN 188
           ++P IAWMDLQG+   +K+ H + D ++++ L  H AT  ER+  PD +D++++
Sbjct: 223 YIPSIAWMDLQGIVRGMKRLHRKFDALLDKKLKEHMATRDERKEKPDLLDVLMD 276


>ref|XP_002314003.1| cytochrome P450 flavonoid 3',5'-hydroxylase [Populus trichocarpa]
           gi|222850411|gb|EEE87958.1| cytochrome P450 flavonoid
           3',5'-hydroxylase [Populus trichocarpa]
          Length = 505

 Score =  226 bits (575), Expect(2) = 2e-68
 Identities = 108/206 (52%), Positives = 153/206 (74%)
 Frame = -2

Query: 965 LLMRHLLRRKSTLLLPPGPRGYPILGALSLIGPAAHVSLANLAKRYGPIMYMKLGSCDVI 786
           LL R L+R+ ST  LPPGPRG+PI+GA+ ++G   H +LA +AK+YGP+MY+K+G+C+++
Sbjct: 19  LLTRCLIRKLSTRQLPPGPRGWPIIGAIPVLGAMPHAALAKMAKQYGPVMYLKMGTCNMV 78

Query: 785 VASTAAAARPFLTTLDLQFSNRPTTIASAKHVSYNGNDFIFANYTPKYKLFRKLSTLHML 606
           VAST  AAR FL TLDL FSNRP   A A H++YN  D +FA+Y P++KL RKLS LHML
Sbjct: 79  VASTPDAARAFLKTLDLNFSNRPPN-AGATHLAYNAQDMVFADYGPRWKLLRKLSNLHML 137

Query: 605 GNKAMAGWADVRRDEVRLMVKSMHESSQLGKLVIVPETLICAMMNIVGRVMLSQRVFDAT 426
           G KA+  WA VR  E+  M+++M E+S+ G+ V+VPE L  AM N++G+++LS+RVF   
Sbjct: 138 GGKALEDWAHVRVSELGHMLRAMCEASRKGEPVVVPEMLTYAMANMIGQIILSRRVFVTK 197

Query: 425 DPELGSYKELLKKLMTGGGLFNIGDF 348
             E   +K+++ +LMT  GLFN+GD+
Sbjct: 198 GSESNEFKDMVVELMTSAGLFNVGDY 223



 Score = 60.5 bits (145), Expect(2) = 2e-68
 Identities = 23/53 (43%), Positives = 39/53 (73%)
 Frame = -3

Query: 349 FVPVIAWMDLQGVQVKLKKGHARMDGMVERMLARHAATVKEREGCPDFIDLVL 191
           ++P +AWMDLQG++  +K+ H R D ++ +M+  H AT  ER+G PDF+D+++
Sbjct: 223 YIPSVAWMDLQGIERGMKRLHRRFDVLLTKMMEEHIATAHERKGKPDFLDVLM 275