BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dioscorea21_contig00021262
         (1121 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002284864.1| PREDICTED: subtilisin-like protease [Vitis v...   461   e-127
ref|XP_002284869.2| PREDICTED: subtilisin-like protease [Vitis v...   460   e-127
emb|CAA59964.1| subtilisin-like protease [Alnus glutinosa]            452   e-125
gb|AAO62352.1| subtilase [Casuarina glauca]                           448   e-123
ref|XP_003624105.1| Xylem serine proteinase [Medicago truncatula...   446   e-123

>ref|XP_002284864.1| PREDICTED: subtilisin-like protease [Vitis vinifera]
          Length = 763

 Score =  461 bits (1187), Expect = e-127
 Identities = 230/377 (61%), Positives = 287/377 (76%), Gaps = 4/377 (1%)
 Frame = -2

Query: 1120 RYFNKGVQAANPGINISMNSARDTYGHGTHCASTAAGNYASA-DYFGYASGVARGIAPRS 944
            RYFNKGV AANPG+N++MNSARDT GHGTH +STAAGNY     YFGYA G ARG+AP +
Sbjct: 197  RYFNKGVIAANPGVNLTMNSARDTQGHGTHTSSTAAGNYVEGVSYFGYAKGTARGVAPGA 256

Query: 943  RLAVYKVNWQEGSYESDVLAGLDQAIADGVDIISVSLGFSGTDFYEDPIAIGSFSAMEKG 764
            R+A+YK  W EG Y SDVLAG+DQA+ADGVD+IS+S+GF     Y+DPIAI SF+AMEKG
Sbjct: 257  RVAMYKALWDEGEYASDVLAGMDQAVADGVDVISISMGFDLVPLYKDPIAIASFAAMEKG 316

Query: 763  IFVSISAGNRGPDRSTLHNGTPWALTVAAGSIDRKFSGTLTLGNGQTIIGTTLYPENALL 584
            + VS SAGN GP   TLHNG PW LTVAAG+IDR F+GTLTLGNG TI G T++P +AL+
Sbjct: 317  VLVSSSAGNEGPSLGTLHNGIPWVLTVAAGTIDRSFAGTLTLGNGLTITGWTMFPASALV 376

Query: 583  VNVQLIYNETISACNSSSLLTSEAEGMIVVCDDTGSTYYQNYYVTESKAAGAVFISDEIH 404
             ++ L+YN+T+SACNSS+LL S A   +V+CD  G  Y Q Y +  SK   A+ ISD+  
Sbjct: 377  QDLPLVYNKTLSACNSSALL-SGAPYAVVICDKVGLIYEQLYQIAASKVGAAIIISDDPE 435

Query: 403  NFD---NTCPGVVINSKQAVNLIKYAKNNLEATVTMKFRQTFIGTERAPAVASSSSRGPS 233
             F+      P V+I+ K A  ++ YAK   + T TM+F+QT + T+ APAVAS +SRGPS
Sbjct: 436  LFELGGVPWPVVMISPKYAKAVVDYAKTAHKPTATMRFQQTLLDTKPAPAVASYTSRGPS 495

Query: 232  QNIPGVLKPDIMAPGTKILAAWVPNIAAAIIGNTPLGSDYNILSGTSMACPHAAGVAALL 53
            ++ PG+LKPD+MAPG+ +LAAW+PN  AAIIG+  L SDYN++SGTSMACPHA+GVAALL
Sbjct: 496  RSYPGILKPDVMAPGSLVLAAWIPNSEAAIIGSLSLSSDYNMISGTSMACPHASGVAALL 555

Query: 52   KSARPGWSPAAIRSAMM 2
            + A P WS AAIRSAM+
Sbjct: 556  RGAHPEWSVAAIRSAMV 572


>ref|XP_002284869.2| PREDICTED: subtilisin-like protease [Vitis vinifera]
            gi|147773976|emb|CAN60787.1| hypothetical protein
            VITISV_034533 [Vitis vinifera]
          Length = 763

 Score =  460 bits (1184), Expect = e-127
 Identities = 232/377 (61%), Positives = 287/377 (76%), Gaps = 4/377 (1%)
 Frame = -2

Query: 1120 RYFNKGVQAANPGINISMNSARDTYGHGTHCASTAAGNYAS-ADYFGYASGVARGIAPRS 944
            RYFNKGV AANPG+N++MNSARDT GHGTH +STAAGNY   A YFGYA G ARG+AP +
Sbjct: 197  RYFNKGVIAANPGVNLTMNSARDTQGHGTHTSSTAAGNYVEGASYFGYAKGTARGVAPGA 256

Query: 943  RLAVYKVNWQEGSYESDVLAGLDQAIADGVDIISVSLGFSGTDFYEDPIAIGSFSAMEKG 764
            R+A+YKV W EG Y SDVLAG+DQA+ADGVD+IS+S+GF     Y+DPIAI SF+AMEKG
Sbjct: 257  RVAMYKVLWDEGRYASDVLAGMDQAVADGVDVISISMGFDLVPLYKDPIAIASFAAMEKG 316

Query: 763  IFVSISAGNRGPDRSTLHNGTPWALTVAAGSIDRKFSGTLTLGNGQTIIGTTLYPENALL 584
            + VS SAGN GP   TLHNG PW LTVAAG+IDR F+GTLTLGNG TI G T++P +AL+
Sbjct: 317  VLVSSSAGNAGPSLGTLHNGIPWVLTVAAGTIDRSFAGTLTLGNGLTIRGWTMFPASALV 376

Query: 583  VNVQLIYNETISACNSSSLLTSEAEGMIVVCDDTGSTYYQNYYVTESKAAGAVFISDEIH 404
             ++ L+YN+T+SACNSS+LL+    G +V+CD  G  Y Q   +  SK   A+ ISD+  
Sbjct: 377  QDLPLVYNKTLSACNSSALLSGAPYG-VVICDKVGFIYEQLDQIAASKVGAAIIISDDPE 435

Query: 403  NFD---NTCPGVVINSKQAVNLIKYAKNNLEATVTMKFRQTFIGTERAPAVASSSSRGPS 233
             F+      P VVI+   A  +I YAK   + T TMKF+QT + T+ APAVAS +SRGPS
Sbjct: 436  LFELGGVPWPVVVISPTYAKAVIDYAKTAHKPTATMKFQQTLLDTKPAPAVASYTSRGPS 495

Query: 232  QNIPGVLKPDIMAPGTKILAAWVPNIAAAIIGNTPLGSDYNILSGTSMACPHAAGVAALL 53
            ++ PG+LKPD+MAPG+ +LAAW+PN  AAIIG+  L SDYN++SGTSMACPHA+GVAALL
Sbjct: 496  RSYPGILKPDVMAPGSLVLAAWIPNSEAAIIGSLSLSSDYNMISGTSMACPHASGVAALL 555

Query: 52   KSARPGWSPAAIRSAMM 2
            + A P WS AAIRSAM+
Sbjct: 556  RGAHPEWSVAAIRSAMV 572


>emb|CAA59964.1| subtilisin-like protease [Alnus glutinosa]
          Length = 761

 Score =  452 bits (1164), Expect = e-125
 Identities = 233/379 (61%), Positives = 282/379 (74%), Gaps = 6/379 (1%)
 Frame = -2

Query: 1120 RYFNKGVQAANPGINISMNSARDTYGHGTHCASTAAGNYAS-ADYFGYASGVARGIAPRS 944
            RYFN G+ AA P    SMNSARDT GHGTH ASTAAGNY + A YFGY  G ARGIAPR+
Sbjct: 190  RYFNNGIMAAIPNATFSMNSARDTLGHGTHTASTAAGNYVNGASYFGYGKGTARGIAPRA 249

Query: 943  RLAVYKVNWQEGSYESDVLAGLDQAIADGVDIISVSLGFSGTDFYEDPIAIGSFSAMEKG 764
            R+AVYKV W EG Y SDVLAG+DQAIADGVD+IS+SLG+ G   YEDPIAI SF+AMEKG
Sbjct: 250  RVAVYKVTWPEGRYTSDVLAGIDQAIADGVDVISISLGYDGVPLYEDPIAIASFAAMEKG 309

Query: 763  IFVSISAGNRGPDRSTLHNGTPWALTVAAGSIDRKFSGTLTLGNGQTIIGTTLYPENALL 584
            + VS SAGN GP    +HNG PW LTVAAG+IDR F+GTLTLGN QTI G T++P +A++
Sbjct: 310  VVVSTSAGNAGPFFGNMHNGIPWVLTVAAGNIDRSFAGTLTLGNDQTITGWTMFPASAII 369

Query: 583  VNVQLIYNETISACNSSSLLTSEAEGMIVVCDDTGSTYYQNYYVTESKAAGAVFISDEIH 404
             + QL+YN+TISACNS+ LL S+A   +V+C+     Y Q   +T S  AGA+ IS+   
Sbjct: 370  ESSQLVYNKTISACNSTELL-SDAVYSVVICEAITPIYAQIDAITRSNVAGAILISNHTK 428

Query: 403  NFD----NTCPGVVINSKQAVNLIKYAKNNLEATVTMKFRQTFIGTERAPAVASSSSRGP 236
             F+     +CP +VI+ K A  LIKYAK +      +KF++T  GT+ APAVA  SSRGP
Sbjct: 429  LFELGGGVSCPCLVISPKDAAALIKYAKTDEFPLAGLKFQETITGTKPAPAVAYYSSRGP 488

Query: 235  SQNIPGVLKPDIMAPGTKILAAWVPNIAAAIIG-NTPLGSDYNILSGTSMACPHAAGVAA 59
            S + PG+LKPD+MAPG+ +LA+W+PN A A IG N  L S YN++SGTSMACPHA+GVAA
Sbjct: 489  SPSYPGILKPDVMAPGSLVLASWIPNEATAQIGTNVYLSSHYNMVSGTSMACPHASGVAA 548

Query: 58   LLKSARPGWSPAAIRSAMM 2
            LLK+A P WSPAAIRSAMM
Sbjct: 549  LLKAAHPEWSPAAIRSAMM 567


>gb|AAO62352.1| subtilase [Casuarina glauca]
          Length = 764

 Score =  448 bits (1153), Expect = e-123
 Identities = 231/378 (61%), Positives = 282/378 (74%), Gaps = 5/378 (1%)
 Frame = -2

Query: 1120 RYFNKGVQAANPGINISMNSARDTYGHGTHCASTAAGNYAS-ADYFGYASGVARGIAPRS 944
            RYFN G+ AANP I   MNSARDT GHGTH ASTAAGNY +   +FGY  G ARGIAPR+
Sbjct: 198  RYFNNGILAANPNITFGMNSARDTIGHGTHTASTAAGNYVNDVSFFGYGKGTARGIAPRA 257

Query: 943  RLAVYKVNWQEGSYESDVLAGLDQAIADGVDIISVSLGFSGTDFYEDPIAIGSFSAMEKG 764
            RLAVYKVNW+EG Y SDVLAG+DQAIADGVD+IS+S+GF G   +EDPIAI SF+AMEKG
Sbjct: 258  RLAVYKVNWREGRYASDVLAGIDQAIADGVDVISISMGFDGAPLHEDPIAIASFAAMEKG 317

Query: 763  IFVSISAGNRGPDRSTLHNGTPWALTVAAGSIDRKFSGTLTLGNGQTIIGTTLYPENALL 584
            + VS SAGN GP    LHNG PW LTVA G++DR F+GTLTLGN Q I G TL+P +A++
Sbjct: 318  VLVSTSAGNEGPFFGNLHNGIPWVLTVAGGTVDRSFAGTLTLGNDQIITGWTLFPASAVI 377

Query: 583  VNVQLIYNETISACNSSSLLTSEAEGMIVVCDDTGSTYYQNYYVTESKAAGAVFISDEIH 404
             N+ L+Y++ ISACNS  LL SEA   I++C+   S   Q   +  S   GA+ IS+  +
Sbjct: 378  QNLPLVYDKNISACNSPELL-SEAIYTIIICEQARSIRDQIDSLARSNVVGAILISNNTN 436

Query: 403  NF---DNTCPGVVINSKQAVNLIKYAKNNLEATVTMKFRQTFIGTERAPAVASSSSRGPS 233
            +    + TCP +VI+ K A  +IKYA  N  A  +MKF++TF+G + APAVAS +SRGPS
Sbjct: 437  SSELGEVTCPCLVISPKDAEAVIKYANFNEIAFASMKFQKTFLGAKPAPAVASYTSRGPS 496

Query: 232  QNIPGVLKPDIMAPGTKILAAWVPNIAAAIIG-NTPLGSDYNILSGTSMACPHAAGVAAL 56
             + PGVLKPD+MAPG++ILAAWVP  A A IG N  L S YN++SGTSMACPHA+G+AAL
Sbjct: 497  PSYPGVLKPDVMAPGSQILAAWVPTDATAQIGTNVYLSSHYNMVSGTSMACPHASGIAAL 556

Query: 55   LKSARPGWSPAAIRSAMM 2
            LK+A P WSPAAIRSAM+
Sbjct: 557  LKAAHPEWSPAAIRSAMI 574


>ref|XP_003624105.1| Xylem serine proteinase [Medicago truncatula]
            gi|87162781|gb|ABD28576.1| Protease-associated PA;
            Proteinase inhibitor I9, subtilisin propeptide [Medicago
            truncatula] gi|355499120|gb|AES80323.1| Xylem serine
            proteinase [Medicago truncatula]
          Length = 769

 Score =  446 bits (1146), Expect = e-123
 Identities = 230/384 (59%), Positives = 287/384 (74%), Gaps = 11/384 (2%)
 Frame = -2

Query: 1120 RYFNKGVQAANPGINISMNSARDTYGHGTHCASTAAGNYAS-ADYFGYASGVARGIAPRS 944
            RYFNKGV A+NP + ISMNSARDT GHGTH +ST AGNY + A YFGYA G+ARGIAP++
Sbjct: 197  RYFNKGVIASNPNVTISMNSARDTIGHGTHTSSTVAGNYVNGASYFGYAKGIARGIAPKA 256

Query: 943  RLAVYKVNWQEGSYESDVLAGLDQAIADGVDIISVSLGFSGTDFYEDPIAIGSFSAMEKG 764
            R+A+YKV W+EG + SDVLAG+DQAI DGVD+IS+S+GF     YEDPIAI SF+AMEKG
Sbjct: 257  RIAMYKVIWEEGRFASDVLAGMDQAINDGVDVISISMGFDDVPLYEDPIAIASFAAMEKG 316

Query: 763  IFVSISAGNRGPDRSTLHNGTPWALTVAAGSIDRKFSGTLTLGNGQTIIGTTLYPENALL 584
            I VS SAGN GP+  TLHNG PW LT AAG+IDR F GTL LGNGQ+IIG TL+P NA++
Sbjct: 317  IVVSSSAGNAGPEFGTLHNGIPWLLTAAAGTIDRTF-GTLVLGNGQSIIGWTLFPANAIV 375

Query: 583  VNVQLIYNETISACNSSSLLTSEAEGMIVVCDDT------GSTYYQNYYVTESKAAGAVF 422
             NV L+YN T+S+CNS +LL+   + +I++CDD+       S + Q   VTE+   GAVF
Sbjct: 376  ENVLLVYNNTLSSCNSLNLLSQLNKKVIILCDDSLSNRNKTSVFNQINVVTEANLLGAVF 435

Query: 421  ISDEIHNFD---NTCPGVVINSKQAVNLIKYAKNNLEATVTMKFRQTFIGTERAPAVASS 251
            +SD     D      P +VI  K A ++I YAK+N   T ++KF+QTF+GT+ APA A  
Sbjct: 436  VSDSPQLIDLGRIYTPSIVIKPKDAQSVINYAKSNNNPTSSIKFQQTFVGTKPAPAAAYY 495

Query: 250  SSRGPSQNIPGVLKPDIMAPGTKILAAWVPNIAAAIIG-NTPLGSDYNILSGTSMACPHA 74
            SSRGPS + P +LKPDIMAPG+++LAA++PN   A IG N  L SDYN +SGTSM+CPH 
Sbjct: 496  SSRGPSHSYPWILKPDIMAPGSRVLAAYIPNKPTARIGTNVFLSSDYNFMSGTSMSCPHV 555

Query: 73   AGVAALLKSARPGWSPAAIRSAMM 2
            +GVAALLK+A P WS AAIRSA++
Sbjct: 556  SGVAALLKAAHPQWSAAAIRSALI 579