BLASTX nr result
ID: Dioscorea21_contig00018416
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dioscorea21_contig00018416
(1295 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002525356.1| sentrin/sumo-specific protease, putative [Ri... 300 3e-91
ref|XP_003551198.1| PREDICTED: ubiquitin-like-specific protease ... 288 3e-89
ref|XP_003530651.1| PREDICTED: LOW QUALITY PROTEIN: ubiquitin-li... 288 6e-89
gb|ABA99256.1| Ulp1 protease family, C-terminal catalytic domain... 278 2e-86
gb|EEC69640.1| hypothetical protein OsI_39038 [Oryza sativa Indi... 278 2e-86
>ref|XP_002525356.1| sentrin/sumo-specific protease, putative [Ricinus communis]
gi|223535319|gb|EEF36994.1| sentrin/sumo-specific
protease, putative [Ricinus communis]
Length = 283
Score = 300 bits (768), Expect(2) = 3e-91
Identities = 143/228 (62%), Positives = 180/228 (78%), Gaps = 2/228 (0%)
Frame = +2
Query: 227 IMNFYIQYLQRSLSSISRARDKYYIFNTYFYEKLKEAHSCEG-DKGASFLKLRRWWKGVN 403
IMNFYI+YL+ S ++A + FNT+FY+KLK+A S +G DK + F+K RRWWKGVN
Sbjct: 47 IMNFYIRYLRLQTSPTNKAISDCHFFNTFFYKKLKQAVSYKGSDKESFFIKFRRWWKGVN 106
Query: 404 IFQKSYIFLPIHGHSHWSLVIICIPAKEDESGPIILHLDSLGIHSSHPIFDIVDSYLIDE 583
IFQK+Y+F+PIH HWSLVIICIP KEDESGPIILHLDSLG+HSS +F+ + SYL E
Sbjct: 107 IFQKAYVFIPIHDDLHWSLVIICIPDKEDESGPIILHLDSLGLHSSKEVFEEIKSYLRQE 166
Query: 584 WNFVNQSAAPLDIPFSRGIWRCLPDWIEKKKIKVPQQKNEYDCGVFVLYYMERFIREAPE 763
WN++NQ AP DIP + IW+ LP IE+KKI+VPQQKN+YDCG+FVLY+MERFI EAPE
Sbjct: 167 WNYMNQEVAPSDIPIAERIWKRLPRRIEEKKIEVPQQKNDYDCGLFVLYFMERFIEEAPE 226
Query: 764 RIRTKDLNMFGNKWFQPQEASNLRNRIRALLLEEFESAR-LDNDEELP 904
R++ KDL MFG +WF+P+EAS LR +IR LLL+EF++A D+ E P
Sbjct: 227 RLKKKDLAMFGKRWFRPEEASGLRVKIRKLLLDEFKNANDTDSVSESP 274
Score = 63.2 bits (152), Expect(2) = 3e-91
Identities = 28/41 (68%), Positives = 34/41 (82%)
Frame = +3
Query: 39 REAKVYYPSRDDPESVELSYSDIKCLDPGSYLTSTIMNFYI 161
++AK+YYPSRDD ESVE+ Y+DI L P S+LTS IMNFYI
Sbjct: 12 KDAKIYYPSRDDRESVEICYTDINSLAPNSFLTSPIMNFYI 52
>ref|XP_003551198.1| PREDICTED: ubiquitin-like-specific protease 1D-like [Glycine max]
Length = 586
Score = 288 bits (736), Expect(2) = 3e-89
Identities = 140/239 (58%), Positives = 181/239 (75%), Gaps = 4/239 (1%)
Frame = +2
Query: 227 IMNFYIQYLQRSLSSISRARDKYYIFNTYFYEKLKEAHSC-EGDKGASFLKLRRWWKGVN 403
IMNFYIQYLQ+ +R+ Y+ FNTYFY+KLKEA S + D+ F K RRWWKGVN
Sbjct: 324 IMNFYIQYLQQQALLTNRSLSDYHFFNTYFYKKLKEAVSYKQSDREMIFAKFRRWWKGVN 383
Query: 404 IFQKSYIFLPIHGHSHWSLVIICIPAKEDESGPIILHLDSLGIHSSHPIFDIVDSYLIDE 583
IFQK+Y+ +PIH HWSL+IICIP KE ESGPIILHLDSLG+HSS +FD + SYLI+E
Sbjct: 384 IFQKAYVLIPIHEDLHWSLIIICIPDKEYESGPIILHLDSLGLHSSKSVFDNIKSYLIEE 443
Query: 584 WNFVNQSAAPLDIPFSRGIWRCLPDWIEKKKIKVPQQKNEYDCGVFVLYYMERFIREAPE 763
N++++ LD+ + IW+CLP IE + I+VPQQKNEYDCG+FVLY++ERF+ EAPE
Sbjct: 444 KNYMDREDVSLDVSIADRIWKCLPRRIESQIIQVPQQKNEYDCGLFVLYFIERFMEEAPE 503
Query: 764 RIRTKDLNMFGNKWFQPQEASNLRNRIRALLLEEFESARLD--NDEELPASS-GCSANC 931
R++ KDL+MFG +WF+PQEASNLR +I LLLE+ +++ D N E P+SS G + +C
Sbjct: 504 RLKRKDLDMFGRRWFKPQEASNLRVKILKLLLEKLQNSITDNCNSESSPSSSAGPATDC 562
Score = 68.9 bits (167), Expect(2) = 3e-89
Identities = 32/49 (65%), Positives = 38/49 (77%), Gaps = 1/49 (2%)
Frame = +3
Query: 18 ETMFHEW-REAKVYYPSRDDPESVELSYSDIKCLDPGSYLTSTIMNFYI 161
E F E+ +EAK+Y+PSRDDPE VE+ Y+D CL P YLTSTIMNFYI
Sbjct: 281 ENKFPEYLKEAKIYFPSRDDPECVEICYTDTNCLAPEGYLTSTIMNFYI 329
>ref|XP_003530651.1| PREDICTED: LOW QUALITY PROTEIN: ubiquitin-like-specific protease
1D-like [Glycine max]
Length = 594
Score = 288 bits (737), Expect(2) = 6e-89
Identities = 138/238 (57%), Positives = 180/238 (75%), Gaps = 3/238 (1%)
Frame = +2
Query: 227 IMNFYIQYLQRSLSSISRARDKYYIFNTYFYEKLKEAHSCEGDKGASFLKLRRWWKGVNI 406
IMNFYIQYLQ+ +R+ Y+ FNTYFY+KLKEA S + F K RRWWKGVNI
Sbjct: 333 IMNFYIQYLQQQALLTNRSLSAYHFFNTYFYKKLKEAVSYKQSDXEIFAKFRRWWKGVNI 392
Query: 407 FQKSYIFLPIHGHSHWSLVIICIPAKEDESGPIILHLDSLGIHSSHPIFDIVDSYLIDEW 586
FQK+Y+ +PIH HWSL+IICIP KEDESGPIILHLDSLG+HSS +FD + SYLI+E
Sbjct: 393 FQKAYVLIPIHEDLHWSLIIICIPDKEDESGPIILHLDSLGLHSSKSVFDNIKSYLIEEK 452
Query: 587 NFVNQSAAPLDIPFSRGIWRCLPDWIEKKKIKVPQQKNEYDCGVFVLYYMERFIREAPER 766
N++++ D+ + IW+CLP IE + I+VPQQKN+YDCG+FVLY++ERF+ EAPER
Sbjct: 453 NYMDREDMASDVSIADRIWKCLPRRIESQIIQVPQQKNDYDCGLFVLYFIERFMEEAPER 512
Query: 767 IRTKDLNMFGNKWFQPQEASNLRNRIRALLLEEFESARLDND--EELPASS-GCSANC 931
++ KDL+MFG +WF+PQEASNLR +IR LL+E+ +++ DN E P+SS G + +C
Sbjct: 513 LKMKDLDMFGRRWFKPQEASNLRVKIRKLLVEKLQNSVTDNCNLESSPSSSAGPATDC 570
Score = 67.4 bits (163), Expect(2) = 6e-89
Identities = 31/49 (63%), Positives = 38/49 (77%), Gaps = 1/49 (2%)
Frame = +3
Query: 18 ETMFHEW-REAKVYYPSRDDPESVELSYSDIKCLDPGSYLTSTIMNFYI 161
E F E+ +EAK+Y+PSRDDPE VE+ ++D CL P YLTSTIMNFYI
Sbjct: 290 ENKFPEYLKEAKIYFPSRDDPECVEICFTDTNCLAPEGYLTSTIMNFYI 338
>gb|ABA99256.1| Ulp1 protease family, C-terminal catalytic domain containing
protein, expressed [Oryza sativa Japonica Group]
Length = 725
Score = 278 bits (710), Expect(2) = 2e-86
Identities = 136/220 (61%), Positives = 166/220 (75%)
Frame = +2
Query: 227 IMNFYIQYLQRSLSSISRARDKYYIFNTYFYEKLKEAHSCEGDKGASFLKLRRWWKGVNI 406
++NFYIQY++R+ R+K+YIFNTYFY KL+EA +G+ FLKLRRWWKGVNI
Sbjct: 348 VINFYIQYMKRTKLHDDDCREKFYIFNTYFYSKLEEALLGKGE----FLKLRRWWKGVNI 403
Query: 407 FQKSYIFLPIHGHSHWSLVIICIPAKEDESGPIILHLDSLGIHSSHPIFDIVDSYLIDEW 586
+ SYI LPIHG +HWSL+IIC P+KE SGPIILHLDSL +HSS IFD V YL EW
Sbjct: 404 YHTSYIILPIHGTAHWSLIIICFPSKESNSGPIILHLDSLELHSSAKIFDTVRRYLEAEW 463
Query: 587 NFVNQSAAPLDIPFSRGIWRCLPDWIEKKKIKVPQQKNEYDCGVFVLYYMERFIREAPER 766
+ ++ P DI S IW LP I+K+K++VPQQKNEYDCG+F+LYY+ERFIR APER
Sbjct: 464 CHLRKNPPP-DISISETIWDDLPSNIQKEKVQVPQQKNEYDCGIFMLYYIERFIRLAPER 522
Query: 767 IRTKDLNMFGNKWFQPQEASNLRNRIRALLLEEFESARLD 886
+L+MF WFQP++AS+LR RIR LLLEEFESARLD
Sbjct: 523 FTRDNLSMFSRSWFQPEDASDLRQRIRELLLEEFESARLD 562
Score = 69.3 bits (168), Expect(2) = 2e-86
Identities = 30/40 (75%), Positives = 35/40 (87%)
Frame = +3
Query: 42 EAKVYYPSRDDPESVELSYSDIKCLDPGSYLTSTIMNFYI 161
E +YYPSRDDPE+VELS SDIKCLDPG YL+S ++NFYI
Sbjct: 314 ETMIYYPSRDDPEAVELSSSDIKCLDPGVYLSSPVINFYI 353
>gb|EEC69640.1| hypothetical protein OsI_39038 [Oryza sativa Indica Group]
Length = 679
Score = 278 bits (710), Expect(2) = 2e-86
Identities = 136/220 (61%), Positives = 166/220 (75%)
Frame = +2
Query: 227 IMNFYIQYLQRSLSSISRARDKYYIFNTYFYEKLKEAHSCEGDKGASFLKLRRWWKGVNI 406
++NFYIQY++R+ R+K+YIFNTYFY KL+EA +G+ FLKLRRWWKGVNI
Sbjct: 299 VINFYIQYMKRTKLHDDDCREKFYIFNTYFYSKLEEALLGKGE----FLKLRRWWKGVNI 354
Query: 407 FQKSYIFLPIHGHSHWSLVIICIPAKEDESGPIILHLDSLGIHSSHPIFDIVDSYLIDEW 586
+ SYI LPIHG +HWSL+IIC P+KE SGPIILHLDSL +HSS IFD V YL EW
Sbjct: 355 YHTSYIILPIHGTAHWSLIIICFPSKESNSGPIILHLDSLELHSSAKIFDTVRRYLEAEW 414
Query: 587 NFVNQSAAPLDIPFSRGIWRCLPDWIEKKKIKVPQQKNEYDCGVFVLYYMERFIREAPER 766
+ ++ P DI S IW LP I+K+K++VPQQKNEYDCG+F+LYY+ERFIR APER
Sbjct: 415 CHLRKNPPP-DISISETIWDDLPSNIQKEKVQVPQQKNEYDCGIFMLYYIERFIRLAPER 473
Query: 767 IRTKDLNMFGNKWFQPQEASNLRNRIRALLLEEFESARLD 886
+L+MF WFQP++AS+LR RIR LLLEEFESARLD
Sbjct: 474 FTRDNLSMFSRSWFQPEDASDLRQRIRELLLEEFESARLD 513
Score = 69.3 bits (168), Expect(2) = 2e-86
Identities = 30/40 (75%), Positives = 35/40 (87%)
Frame = +3
Query: 42 EAKVYYPSRDDPESVELSYSDIKCLDPGSYLTSTIMNFYI 161
E +YYPSRDDPE+VELS SDIKCLDPG YL+S ++NFYI
Sbjct: 265 ETMIYYPSRDDPEAVELSSSDIKCLDPGVYLSSPVINFYI 304