BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dioscorea21_contig00018082
         (540 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_003576075.1| PREDICTED: 4-coumarate--CoA ligase-like 7-li...   123   2e-26
ref|NP_001059373.1| Os07g0280200 [Oryza sativa Japonica Group] g...   119   3e-25
gb|EAZ03495.1| hypothetical protein OsI_25635 [Oryza sativa Indi...   119   3e-25
gb|AGE10595.1| 4-coumarate CoA ligase [Lonicera japonica]             117   9e-25
gb|ADN33954.1| 4-coumarate-CoA ligase [Cucumis melo subsp. melo]      116   3e-24

>ref|XP_003576075.1| PREDICTED: 4-coumarate--CoA ligase-like 7-like [Brachypodium
           distachyon]
          Length = 583

 Score =  123 bits (308), Expect = 2e-26
 Identities = 78/202 (38%), Positives = 109/202 (53%), Gaps = 25/202 (12%)
 Frame = -1

Query: 534 SHLGISKGHVAFILSPSCLEIPILYLSLLSLGXXXXXXXXXXXPSELSRLIDLSSPTHFF 355
           S LG+ +G VAF+L+P+ L++P+LY +LLSLG            +E+ RL+ LS  +  F
Sbjct: 99  SQLGLGRGDVAFVLAPARLDVPVLYFALLSLGAVVSPANPALTAAEVERLVSLSGASVAF 158

Query: 354 ATSSSSSNLPLHVTPILIDSPLFRSFL-------ESDSISHLSDPIEIHQSDPAAILFXX 196
           A SS+++ LP ++  +L+DSP FRS L        SD+  H   PI I QS  A I +  
Sbjct: 159 AVSSTAAKLPANLPVVLLDSPHFRSLLNPNGDQESSDAADHAVIPIPIPQSATATIQYSS 218

Query: 195 XXXXXTKAAAISHRNIIAVTAVMRA-----------IRPSEK------ETTLLIGPMFHS 67
                 KAAA+ HR+ IA++A   A             PS K      + TLL  PMFHS
Sbjct: 219 GTTGRVKAAALPHRSFIAMSAGFHAHALRQRRRADDTNPSNKSPGQHHQITLLGAPMFHS 278

Query: 66  MGFFMVMNNV-MRQETVVVARP 4
           MGFF  +N + + Q TVV+  P
Sbjct: 279 MGFFFTLNGIALGQTTVVMTEP 300


>ref|NP_001059373.1| Os07g0280200 [Oryza sativa Japonica Group]
           gi|75289692|sp|Q69RG7.1|4CLL7_ORYSJ RecName:
           Full=4-coumarate--CoA ligase-like 7
           gi|50508642|dbj|BAD31128.1| putative 4-coumarate--CoA
           ligase 1 [Oryza sativa Japonica Group]
           gi|113610909|dbj|BAF21287.1| Os07g0280200 [Oryza sativa
           Japonica Group] gi|215766251|dbj|BAG98479.1| unnamed
           protein product [Oryza sativa Japonica Group]
          Length = 558

 Score =  119 bits (298), Expect = 3e-25
 Identities = 72/181 (39%), Positives = 105/181 (58%), Gaps = 5/181 (2%)
 Frame = -1

Query: 540 LHSHLGISKGHVAFILSPSCLEIPILYLSLLSLGXXXXXXXXXXXPSELSRLIDLSSPTH 361
           L S +G+  G VAF+L+P+ L++P+LY +LLS+G           P+E+SRL+ LS  + 
Sbjct: 95  LRSRVGLRGGDVAFVLAPAGLDVPVLYFALLSIGAVVSPANPALTPAEVSRLVSLSGASV 154

Query: 360 FFATSSSSSNLPLHVTP-ILIDSPLFRSFLESDSISHLSDP---IEIHQSDPAAILFXXX 193
            FA SS+++ LP  +T  +L+DSP FRS L     +   +P   + + QS+ AAI +   
Sbjct: 155 AFAVSSTATKLPAGLTTVVLLDSPHFRSLLMDCGQAQGQEPLPVVVVRQSETAAIQYSSG 214

Query: 192 XXXXTKAAAISHRNIIAVTAVMRAIRPSEKETTLLIG-PMFHSMGFFMVMNNVMRQETVV 16
                KAAA+ HR+ IA+ A   A+R   +E   L+G PMFHSMGF  V+  V    T V
Sbjct: 215 TTGRVKAAALPHRSFIAMVAGFHALRAKAREVRTLLGAPMFHSMGFLFVLQGVALGATTV 274

Query: 15  V 13
           V
Sbjct: 275 V 275


>gb|EAZ03495.1| hypothetical protein OsI_25635 [Oryza sativa Indica Group]
          Length = 552

 Score =  119 bits (298), Expect = 3e-25
 Identities = 72/181 (39%), Positives = 105/181 (58%), Gaps = 5/181 (2%)
 Frame = -1

Query: 540 LHSHLGISKGHVAFILSPSCLEIPILYLSLLSLGXXXXXXXXXXXPSELSRLIDLSSPTH 361
           L S +G+  G VAF+L+P+ L++P+LY +LLS+G           P+E+SRL+ LS  + 
Sbjct: 89  LRSRVGLRGGDVAFVLAPAGLDVPVLYFALLSIGAVVSPANPALTPAEVSRLVSLSGASV 148

Query: 360 FFATSSSSSNLPLHVTP-ILIDSPLFRSFLESDSISHLSDP---IEIHQSDPAAILFXXX 193
            FA SS+++ LP  +T  +L+DSP FRS L     +   +P   + + QS+ AAI +   
Sbjct: 149 AFAVSSTATKLPAGLTTVVLLDSPHFRSLLMDCGQAQGQEPLPVVVVRQSETAAIQYSSG 208

Query: 192 XXXXTKAAAISHRNIIAVTAVMRAIRPSEKETTLLIG-PMFHSMGFFMVMNNVMRQETVV 16
                KAAA+ HR+ IA+ A   A+R   +E   L+G PMFHSMGF  V+  V    T V
Sbjct: 209 TTGRVKAAALPHRSFIAMVAGFHALRAKAREVRTLLGAPMFHSMGFLFVLQGVALGATTV 268

Query: 15  V 13
           V
Sbjct: 269 V 269


>gb|AGE10595.1| 4-coumarate CoA ligase [Lonicera japonica]
          Length = 540

 Score =  117 bits (294), Expect = 9e-25
 Identities = 77/179 (43%), Positives = 102/179 (56%), Gaps = 3/179 (1%)
 Frame = -1

Query: 540 LHSHLGISKGHVAFILSPSCLEIPILYLSLLSLGXXXXXXXXXXXPSELSRLIDLSSPTH 361
           L SHLG+SKG VAFILS + L+IP+LY SLLSLG            SE+SR I LS P  
Sbjct: 79  LQSHLGLSKGDVAFILSLNSLQIPLLYFSLLSLGVIISPSNPSSTESEISRQIKLSKPVI 138

Query: 360 FFATSSSSSNLP-LHVTPILIDSPLFRSFLESDSISHLSDPIEIHQSDPAAILFXXXXXX 184
            FATS++S  +P L    +LIDSP F S + + S   LS  + + Q D AAI++      
Sbjct: 139 AFATSATSHKIPALRHGTVLIDSPEFVS-MTTSSGRELS-CVGVSQCDIAAIMYSSGTTG 196

Query: 183 XTKAAAISHRNIIAVTAVMRAIRPSEKETTLLI--GPMFHSMGFFMVMNNVMRQETVVV 13
             K   ++HRN+ A+ A   A+RP  K   +++   P FH +G F  M +V   ETVVV
Sbjct: 197 QVKGVMLTHRNLTAIVANYHALRPERKSPAVVLYTMPYFHVIGLFCCMKSVALSETVVV 255


>gb|ADN33954.1| 4-coumarate-CoA ligase [Cucumis melo subsp. melo]
          Length = 1055

 Score =  116 bits (290), Expect = 3e-24
 Identities = 76/184 (41%), Positives = 100/184 (54%), Gaps = 8/184 (4%)
 Frame = -1

Query: 540  LHSHLGISKGHVAFILSPSCLEIPILYLSLLSLGXXXXXXXXXXXPSELSRLIDLSSPTH 361
            L S   +  GHVAFIL+P+ L+IP+LY +LLSLG            SE+S  I LS P  
Sbjct: 577  LKSLTSLCNGHVAFILAPTSLQIPVLYFALLSLGVVLSPANPTSSVSEISHQIQLSKPVI 636

Query: 360  FFATSSSSSNLP-LHVTPILIDSPLFRSFLESDSISHLSDPIEIHQSDPAAILFXXXXXX 184
             FATSS++S LP L    ++IDSP F S L   + S     I+I QSD AAIL+      
Sbjct: 637  AFATSSTASKLPTLRFGTVIIDSPHFLSMLTETNGSDGLTDIKIDQSDSAAILYSSGTTG 696

Query: 183  XTKAAAISHRNIIAV----TAVMRAIRPSEKE---TTLLIGPMFHSMGFFMVMNNVMRQE 25
              K   +SHRN+IAV    TA    I   E +     L + P+FH  GF M++  + R E
Sbjct: 697  RVKGVLLSHRNLIAVNSGPTAFQSEIHEGEMKPHPVALCLLPLFHVFGFVMLVRAISRGE 756

Query: 24   TVVV 13
            T+V+
Sbjct: 757  TLVL 760



 Score =  102 bits (254), Expect = 4e-20
 Identities = 70/185 (37%), Positives = 99/185 (53%), Gaps = 14/185 (7%)
 Frame = -1

Query: 519 SKGHVAFILSPSCLEIPILYLSLLSLGXXXXXXXXXXXPSELSRLIDLSSPTHFFATSSS 340
           S G VAFILSP+ L+IP+LY +LLSLG            SE++  + L  P   FA SS+
Sbjct: 89  SNGQVAFILSPTSLQIPVLYFALLSLGVVVSPANPISSESEIAHQVSLCKPVIAFAISST 148

Query: 339 SSNLP-LHVTPILIDSPLFRSFL-ESDSISHLSDPI---EIHQSDPAAILFXXXXXXXTK 175
           +S +P L +  +LIDSP F S + ES+    ++D I   +I+Q+D AAIL+        K
Sbjct: 149 ASKIPRLPLGTVLIDSPEFLSLMNESNRSEGVNDGIFDLKINQNDSAAILYSSGTTGRVK 208

Query: 174 AAAISHRNIIAV---------TAVMRAIRPSEKETTLLIGPMFHSMGFFMVMNNVMRQET 22
              +SHRN+I           T V   I P     +LL  P+FH  GFFM+  ++    T
Sbjct: 209 GVLLSHRNLITAITGVQVLDKTPVDGEIEPHPVALSLL--PLFHVFGFFMLFRSISEGNT 266

Query: 21  VVVAR 7
           +V+ R
Sbjct: 267 LVLMR 271