BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dioscorea21_contig00015371
         (2136 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002274214.2| PREDICTED: L-galactono-1,4-lactone dehydroge...   920   0.0  
gb|ACN59217.1| putative L-galactono-1,4-lactone dehydrogenase [V...   916   0.0  
gb|AAT67210.1| mitochondrial L-galactono-1,4-lactone dehydrogena...   909   0.0  
gb|ACG75919.1| L-galactono-1,4-lactone dehydrogenase [Malpighia ...   906   0.0  
gb|ADT80722.1| L-galactono-1,4-lactone dehydrogenase [Cucumis sa...   906   0.0  

>ref|XP_002274214.2| PREDICTED: L-galactono-1,4-lactone dehydrogenase, mitochondrial
            [Vitis vinifera]
          Length = 609

 Score =  920 bits (2379), Expect = 0.0
 Identities = 443/568 (77%), Positives = 503/568 (88%), Gaps = 12/568 (2%)
 Frame = +2

Query: 2    PNPNPNLN---FIRSVSTSSDGELRKYAGYFVLLLGCGVATYYSFPLPENAKHKKAQIFR 172
            PNPNPNL       S S SS+ E RKY GYF LLLGCG+ATYYSFP  ++AKHKKAQ+FR
Sbjct: 41   PNPNPNLTRPFSSLSSSPSSEAEFRKYLGYFALLLGCGIATYYSFPFSDSAKHKKAQLFR 100

Query: 173  YAPLPDDLHTVSNWSGTHEVSTRTFLQPESLRDLEVVVRDAHQSKQKIRPVGSGLSPNGI 352
            YAPLPDDLHTVSNWSGTHEV TR F QPESL +LE +V++A++ KQKIRPVGSGLSPNGI
Sbjct: 101  YAPLPDDLHTVSNWSGTHEVQTRVFHQPESLEELEQIVKEANEKKQKIRPVGSGLSPNGI 160

Query: 353  GLQRAGMINLALMDKVLEVDREKKRVRVQAGIRVSQLVDELKEHGLTLQNFASIREQQIG 532
            GL RAGM+NLALMD VL+VD EKKRVRVQAGIRV QLVD +K++G+TLQNFASIREQQIG
Sbjct: 161  GLTRAGMVNLALMDNVLDVDVEKKRVRVQAGIRVQQLVDAIKDYGITLQNFASIREQQIG 220

Query: 533  GIIQVGAHGTGASLPPIDEQIVSMKLVTPAKGTIEISKEQNPELFYLARCGLGGLGVVAE 712
            GI+QVGAHGTGA LPPIDEQ++SMKLVTPAKGTIE+SKE++PELFYLARCGLGGLGVVAE
Sbjct: 221  GIVQVGAHGTGARLPPIDEQVISMKLVTPAKGTIEVSKERDPELFYLARCGLGGLGVVAE 280

Query: 713  VTIQCVDRHELLEHTFVSDLKEIRKNHKRWLAENKHVKYLWIPYTDTIVVVQCNPLSKWK 892
            VT+QCV+R EL+EHT VS++KEI+KNHK+ L+ENKHVKYL+IPYTDT+VVV CNP+SKWK
Sbjct: 281  VTLQCVERQELVEHTVVSNMKEIKKNHKKLLSENKHVKYLYIPYTDTVVVVTCNPVSKWK 340

Query: 893  SPPKFTPKYSEDEAVQHVRDLYRDSLRKYRAE---------ESADINQLSFTELRDKLIA 1045
             PPKF PKYS DEA+QHVRDLY++SL+KY  E            DIN+LSFTELRDKL+A
Sbjct: 341  GPPKFKPKYSRDEAIQHVRDLYQESLKKYSPEAITAKSSDNSEPDINELSFTELRDKLLA 400

Query: 1046 LDPLNKDHIIKVNQAEAEFWRKSEGYRVGWSDEILGFDCGGQQWVSETCFPSGTLAKPSM 1225
            LDPLNKDH+I+VNQAEAEFWRKSEGYRVGWSDEILGFDCGGQQWVSETCFP+GTLAKPSM
Sbjct: 401  LDPLNKDHVIQVNQAEAEFWRKSEGYRVGWSDEILGFDCGGQQWVSETCFPAGTLAKPSM 460

Query: 1226 RDLDYMEELKQLVETEGIPAPAPIEQRWTARSLSTMSPASSPRADDIFSWVGIIMYLPTT 1405
            +DL+Y+E+LK+L+E E IPAPAPIEQRWT  S S MSPASS   DDIFSWVGIIMYLPT 
Sbjct: 461  KDLEYIEDLKKLIEKEEIPAPAPIEQRWTQSSKSPMSPASSSAEDDIFSWVGIIMYLPTM 520

Query: 1406 DARQRKDITEKFFHYRHLAQSKLWDEYSAYEHWAKIEVPKDKDILSALQERLRKRFPVDD 1585
            DARQRK+ITE+FFHYR L+Q++LWD YSAYEHWAKIEVPKDKD L+ALQ RLRKRFPVD+
Sbjct: 521  DARQRKEITEEFFHYRRLSQTQLWDLYSAYEHWAKIEVPKDKDELAALQARLRKRFPVDE 580

Query: 1586 YNKARQELDPNKILSNRVLEKLFPSQET 1669
            YNKAR+ELDPN+ILSN +L KLFPS +T
Sbjct: 581  YNKARKELDPNRILSNNMLGKLFPSSDT 608


>gb|ACN59217.1| putative L-galactono-1,4-lactone dehydrogenase [Vitis vinifera]
          Length = 611

 Score =  916 bits (2368), Expect = 0.0
 Identities = 441/568 (77%), Positives = 502/568 (88%), Gaps = 12/568 (2%)
 Frame = +2

Query: 2    PNPNPNLN---FIRSVSTSSDGELRKYAGYFVLLLGCGVATYYSFPLPENAKHKKAQIFR 172
            PNPNPNL       S S SS+ E RKY GYF LLLGCG+ATYYSFP  ++AKHKKAQ+FR
Sbjct: 43   PNPNPNLTRPFSSLSSSPSSEAEFRKYLGYFALLLGCGIATYYSFPFSDSAKHKKAQLFR 102

Query: 173  YAPLPDDLHTVSNWSGTHEVSTRTFLQPESLRDLEVVVRDAHQSKQKIRPVGSGLSPNGI 352
            YAPLPDDLHTVSNWSGTHEV TR F QPESL +LE +V++A++ KQKIRPVGSGLSPNGI
Sbjct: 103  YAPLPDDLHTVSNWSGTHEVQTRVFHQPESLEELEQIVKEANEKKQKIRPVGSGLSPNGI 162

Query: 353  GLQRAGMINLALMDKVLEVDREKKRVRVQAGIRVSQLVDELKEHGLTLQNFASIREQQIG 532
            GL RAGM+NLAL+D VL+VD EKKRVRVQAGIRV QLVD +K++G+TLQNFASIREQQIG
Sbjct: 163  GLTRAGMVNLALLDNVLDVDVEKKRVRVQAGIRVQQLVDAIKDYGITLQNFASIREQQIG 222

Query: 533  GIIQVGAHGTGASLPPIDEQIVSMKLVTPAKGTIEISKEQNPELFYLARCGLGGLGVVAE 712
            GI+QVGAHGTGA LPPIDEQ++SMKLVTPAKGTIE+SKE++PELFYLARCGLGGLGVVAE
Sbjct: 223  GIVQVGAHGTGARLPPIDEQVISMKLVTPAKGTIEVSKEKDPELFYLARCGLGGLGVVAE 282

Query: 713  VTIQCVDRHELLEHTFVSDLKEIRKNHKRWLAENKHVKYLWIPYTDTIVVVQCNPLSKWK 892
            VT+QCV+R EL+EHT VS++KEI+KNHK+ L+ENKHVKYL+IPYTDT+VVV CNP+SKWK
Sbjct: 283  VTLQCVERQELVEHTVVSNMKEIKKNHKKLLSENKHVKYLYIPYTDTVVVVTCNPVSKWK 342

Query: 893  SPPKFTPKYSEDEAVQHVRDLYRDSLRKYRAE---------ESADINQLSFTELRDKLIA 1045
             PPKF PKYS DEA+QHVR LY++SL+KY  E            DIN+LSFTELRDKL+A
Sbjct: 343  GPPKFKPKYSHDEAIQHVRHLYQESLKKYSPEAITAKSSDNSEPDINELSFTELRDKLLA 402

Query: 1046 LDPLNKDHIIKVNQAEAEFWRKSEGYRVGWSDEILGFDCGGQQWVSETCFPSGTLAKPSM 1225
            LDPLNKDH+I+VNQAEAEFWRKSEGYRVGWSDEILGFDCGGQQWVSETCFP+GTLAKPSM
Sbjct: 403  LDPLNKDHVIQVNQAEAEFWRKSEGYRVGWSDEILGFDCGGQQWVSETCFPAGTLAKPSM 462

Query: 1226 RDLDYMEELKQLVETEGIPAPAPIEQRWTARSLSTMSPASSPRADDIFSWVGIIMYLPTT 1405
            +DL+Y+E+LK+L+E E IPAPAPIEQRWTA S S MSPASS   DDIFSWVGIIMYLPT 
Sbjct: 463  KDLEYIEDLKKLIEKEEIPAPAPIEQRWTASSKSPMSPASSSAEDDIFSWVGIIMYLPTM 522

Query: 1406 DARQRKDITEKFFHYRHLAQSKLWDEYSAYEHWAKIEVPKDKDILSALQERLRKRFPVDD 1585
            DARQRK+ITE+FFHYR L+Q++LWD YSAYEHWAKIEVPKDKD L+ALQ RLRKRFPVD+
Sbjct: 523  DARQRKEITEEFFHYRRLSQTQLWDLYSAYEHWAKIEVPKDKDELAALQARLRKRFPVDE 582

Query: 1586 YNKARQELDPNKILSNRVLEKLFPSQET 1669
            YNKAR+ELDPN+ILSN +  KLFPS +T
Sbjct: 583  YNKARKELDPNRILSNNMQGKLFPSSDT 610


>gb|AAT67210.1| mitochondrial L-galactono-1,4-lactone dehydrogenase [Rosa roxburghii]
          Length = 589

 Score =  909 bits (2349), Expect = 0.0
 Identities = 437/558 (78%), Positives = 498/558 (89%), Gaps = 2/558 (0%)
 Frame = +2

Query: 2    PNPNPNLNFIRSVSTSSDGELRKYAGYFVLLLGCGVATYYSFPLPENAKHKKAQIFRYAP 181
            P+P P+     S +++S  E+RKY GY  L+  CG ATYYSFP PENAKHKKAQIFRYAP
Sbjct: 35   PSPAPSA----SSASASASEVRKYMGYTALVSFCGAATYYSFPFPENAKHKKAQIFRYAP 90

Query: 182  LPDDLHTVSNWSGTHEVSTRTFLQPESLRDLEVVVRDAHQSKQKIRPVGSGLSPNGIGLQ 361
            LP++LHTVSNWSGTHEV TR F QPE+L +LE VV++A+++K +IRPVGSGLSPNGIG  
Sbjct: 91   LPEELHTVSNWSGTHEVQTRVFHQPETLEELEKVVKEANENKYRIRPVGSGLSPNGIGSS 150

Query: 362  RAGMINLALMDKVLEVDREKKRVRVQAGIRVSQLVDELKEHGLTLQNFASIREQQIGGII 541
            RAGM+NLALMDKVLEVD+EKKRVRVQAGIRV QLVD +K+ GLTLQNFASIREQQIGGI+
Sbjct: 151  RAGMVNLALMDKVLEVDKEKKRVRVQAGIRVQQLVDGIKDQGLTLQNFASIREQQIGGIV 210

Query: 542  QVGAHGTGASLPPIDEQIVSMKLVTPAKGTIEISKEQNPELFYLARCGLGGLGVVAEVTI 721
            QVGAHGTGA LPPIDEQ++SMKLVTPAKGTIE+SKE++PELFYLARCGLGGLGVVAEVTI
Sbjct: 211  QVGAHGTGARLPPIDEQVISMKLVTPAKGTIEVSKEKDPELFYLARCGLGGLGVVAEVTI 270

Query: 722  QCVDRHELLEHTFVSDLKEIRKNHKRWLAENKHVKYLWIPYTDTIVVVQCNPLSKWKSPP 901
            QCVDR ELLEHT VS+++EI+KNHK+ L+ENKHVKYL+IPYTDT+VVV CNP+SKWK PP
Sbjct: 271  QCVDRQELLEHTIVSNMEEIKKNHKKLLSENKHVKYLYIPYTDTVVVVTCNPVSKWKGPP 330

Query: 902  KFTPKYSEDEAVQHVRDLYRDSLRKYRA--EESADINQLSFTELRDKLIALDPLNKDHII 1075
            KF PKY+ DEA+QHVRDLYR+ L+KYR   + S DIN+LSFTELRDKL+AL+PLNKDHI+
Sbjct: 331  KFKPKYTTDEAIQHVRDLYRECLKKYRVVPDNSVDINELSFTELRDKLLALNPLNKDHIV 390

Query: 1076 KVNQAEAEFWRKSEGYRVGWSDEILGFDCGGQQWVSETCFPSGTLAKPSMRDLDYMEELK 1255
            KVNQAEAEFWRKSEGYRVGWSDEILGFDCGGQQWVSETCFP+GTLAKPSM+DL+Y+ +LK
Sbjct: 391  KVNQAEAEFWRKSEGYRVGWSDEILGFDCGGQQWVSETCFPAGTLAKPSMKDLEYIADLK 450

Query: 1256 QLVETEGIPAPAPIEQRWTARSLSTMSPASSPRADDIFSWVGIIMYLPTTDARQRKDITE 1435
            QL+E E IPAPAPIEQRWTA +   MSPASS R DDIFSWVGIIMYLPTTDARQRKDITE
Sbjct: 451  QLIEKEEIPAPAPIEQRWTASTKGPMSPASSSREDDIFSWVGIIMYLPTTDARQRKDITE 510

Query: 1436 KFFHYRHLAQSKLWDEYSAYEHWAKIEVPKDKDILSALQERLRKRFPVDDYNKARQELDP 1615
            +FFHYRHL Q++LWD YS+YEHWAKIEVPKD++ L+ALQ RLRKRFPVD YNKAR ELDP
Sbjct: 511  EFFHYRHLTQTRLWDTYSSYEHWAKIEVPKDREQLTALQARLRKRFPVDAYNKARTELDP 570

Query: 1616 NKILSNRVLEKLFPSQET 1669
            N+ILSN  LEKLFP  +T
Sbjct: 571  NRILSNIKLEKLFPLSDT 588


>gb|ACG75919.1| L-galactono-1,4-lactone dehydrogenase [Malpighia glabra]
          Length = 592

 Score =  906 bits (2342), Expect = 0.0
 Identities = 431/560 (76%), Positives = 497/560 (88%), Gaps = 9/560 (1%)
 Frame = +2

Query: 8    PNPNLNFIRSVSTSSDGELRKYAGYFVLLLGCGVATYYSFPLPENAKHKKAQIFRYAPLP 187
            P P   F  S  T++D E+RKY GY  L + CG ATYYSFP  ENAKHKKAQIFRYAPLP
Sbjct: 29   PTPTRTFCTSPPTATDSEVRKYLGYTALFIFCGAATYYSFPFSENAKHKKAQIFRYAPLP 88

Query: 188  DDLHTVSNWSGTHEVSTRTFLQPESLRDLEVVVRDAHQSKQKIRPVGSGLSPNGIGLQRA 367
            +DLHTVSNWSGTHEV TR F QPE++ +LE +V+ +++ K++IRPVGSGLSPNGIGL R 
Sbjct: 89   EDLHTVSNWSGTHEVQTRNFHQPETINELEELVKVSNEKKERIRPVGSGLSPNGIGLSRL 148

Query: 368  GMINLALMDKVLEVDREKKRVRVQAGIRVSQLVDELKEHGLTLQNFASIREQQIGGIIQV 547
            GM+NLAL+DKVLEVD+EKKRVRVQAGIRV +LVD +KEHGLTLQNFASIREQQIGGI+QV
Sbjct: 149  GMVNLALVDKVLEVDKEKKRVRVQAGIRVQELVDGIKEHGLTLQNFASIREQQIGGIVQV 208

Query: 548  GAHGTGASLPPIDEQIVSMKLVTPAKGTIEISKEQNPELFYLARCGLGGLGVVAEVTIQC 727
            GAHGTGA LPPIDEQ++SMKLVTPAKGTIEISK+++PELFYLARCGLGGLGVVAEVT+QC
Sbjct: 209  GAHGTGARLPPIDEQVISMKLVTPAKGTIEISKDKDPELFYLARCGLGGLGVVAEVTLQC 268

Query: 728  VDRHELLEHTFVSDLKEIRKNHKRWLAENKHVKYLWIPYTDTIVVVQCNPLSKWKSPPKF 907
            V+R +L+EHT++S++K+IRKNHK+ L++NKHVKYL+IPYTD +VVV CNP+SKW+  PKF
Sbjct: 269  VERQQLVEHTYISNMKDIRKNHKKLLSDNKHVKYLYIPYTDAVVVVTCNPVSKWRGVPKF 328

Query: 908  TPKYSEDEAVQHVRDLYRDSLRKYRA---------EESADINQLSFTELRDKLIALDPLN 1060
            TPKY+EDEA+QHVRDLY++ L KYR          ++S DIN+LSFTELRDKL+ALDPLN
Sbjct: 329  TPKYTEDEALQHVRDLYQEPLNKYRGGEITSKSSEDDSPDINELSFTELRDKLLALDPLN 388

Query: 1061 KDHIIKVNQAEAEFWRKSEGYRVGWSDEILGFDCGGQQWVSETCFPSGTLAKPSMRDLDY 1240
            KDH+IKVNQAEAEFWRKSEGYRVGWSDEILGFDCGGQQWVSETCFP+GTLA PSM+DLDY
Sbjct: 389  KDHVIKVNQAEAEFWRKSEGYRVGWSDEILGFDCGGQQWVSETCFPAGTLANPSMQDLDY 448

Query: 1241 MEELKQLVETEGIPAPAPIEQRWTARSLSTMSPASSPRADDIFSWVGIIMYLPTTDARQR 1420
            +E+LKQL+E E IPAPAPIEQRWTARS S+MS ASS + DDIFSWVGIIMYLPT DARQR
Sbjct: 449  IEDLKQLIEKEDIPAPAPIEQRWTARSQSSMSVASSSKEDDIFSWVGIIMYLPTMDARQR 508

Query: 1421 KDITEKFFHYRHLAQSKLWDEYSAYEHWAKIEVPKDKDILSALQERLRKRFPVDDYNKAR 1600
            K+ITE+FFHYRHL Q++LWD+YSAYEHWAKIEVPKDK+ L AL ERL+KRFPVD YNKAR
Sbjct: 509  KEITEEFFHYRHLTQAELWDKYSAYEHWAKIEVPKDKEELEALLERLKKRFPVDAYNKAR 568

Query: 1601 QELDPNKILSNRVLEKLFPS 1660
            +ELDPNKILSN  LEKLFPS
Sbjct: 569  KELDPNKILSNNKLEKLFPS 588


>gb|ADT80722.1| L-galactono-1,4-lactone dehydrogenase [Cucumis sativus]
          Length = 590

 Score =  906 bits (2341), Expect = 0.0
 Identities = 429/543 (79%), Positives = 492/543 (90%)
 Frame = +2

Query: 41   STSSDGELRKYAGYFVLLLGCGVATYYSFPLPENAKHKKAQIFRYAPLPDDLHTVSNWSG 220
            S+S D ELRKY GY  L++ CG ATYYSFP PENAKHKKAQIFRYAPLP+DLHTVSNWSG
Sbjct: 47   SSSFDSELRKYLGYGALVIFCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSG 106

Query: 221  THEVSTRTFLQPESLRDLEVVVRDAHQSKQKIRPVGSGLSPNGIGLQRAGMINLALMDKV 400
            THEV TR F QPE+L  LE VV+ A++ K +IRPVGSGLSPNGIGL R GM+NLALMDKV
Sbjct: 107  THEVWTRVFHQPENLEQLEQVVKQANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKV 166

Query: 401  LEVDREKKRVRVQAGIRVSQLVDELKEHGLTLQNFASIREQQIGGIIQVGAHGTGASLPP 580
            LEVD+EKKRVRVQAGIRV QLVD +KE+GLTLQNFASIREQQIGGIIQVGAHGTGA LPP
Sbjct: 167  LEVDKEKKRVRVQAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPP 226

Query: 581  IDEQIVSMKLVTPAKGTIEISKEQNPELFYLARCGLGGLGVVAEVTIQCVDRHELLEHTF 760
            IDEQ+++MKLVTPAKGTIEISK+++PELF LARCGLGGLGVVAEVT+QCV+R EL+EHT+
Sbjct: 227  IDEQVIAMKLVTPAKGTIEISKDKDPELFLLARCGLGGLGVVAEVTLQCVERQELVEHTY 286

Query: 761  VSDLKEIRKNHKRWLAENKHVKYLWIPYTDTIVVVQCNPLSKWKSPPKFTPKYSEDEAVQ 940
            +S++K+I+KNHK+ LA+NKHVKYL+IPYTD IVVV CNP+SKW+ PPKF PKY+ +EA+Q
Sbjct: 287  ISNMKDIKKNHKKLLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQ 346

Query: 941  HVRDLYRDSLRKYRAEESADINQLSFTELRDKLIALDPLNKDHIIKVNQAEAEFWRKSEG 1120
            HVRDLY +SL+KY A E  D+N++SFTELRDKL+ALDPLNK+H+IKVNQAEAEFWRKSEG
Sbjct: 347  HVRDLYVESLKKYSASEERDMNEISFTELRDKLLALDPLNKEHVIKVNQAEAEFWRKSEG 406

Query: 1121 YRVGWSDEILGFDCGGQQWVSETCFPSGTLAKPSMRDLDYMEELKQLVETEGIPAPAPIE 1300
            YRVGWSDEILGFDCGGQQWVSETCFP+GTLAKP+M+D++Y+EELKQL+E + IPAPAP+E
Sbjct: 407  YRVGWSDEILGFDCGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKKNIPAPAPLE 466

Query: 1301 QRWTARSLSTMSPASSPRADDIFSWVGIIMYLPTTDARQRKDITEKFFHYRHLAQSKLWD 1480
            QRWTARS S MSPASS   DDIFSWVGIIMYLPT+DARQRK+ITE+FFHYRHL Q+ LWD
Sbjct: 467  QRWTARSKSPMSPASSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWD 526

Query: 1481 EYSAYEHWAKIEVPKDKDILSALQERLRKRFPVDDYNKARQELDPNKILSNRVLEKLFPS 1660
            +YSA+EHWAKIEVPKDKD L+ALQ RLRKRFPVD+YNKAR+ LDPNKILSN  LEKLF S
Sbjct: 527  QYSAFEHWAKIEVPKDKDELAALQARLRKRFPVDEYNKARRALDPNKILSNNKLEKLFSS 586

Query: 1661 QET 1669
             +T
Sbjct: 587  TDT 589