BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dioscorea21_contig00014409
         (1435 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CBI30065.3| unnamed protein product [Vitis vinifera]              391   e-106
ref|XP_002274685.1| PREDICTED: folylpolyglutamate synthase, mito...   391   e-106
ref|XP_002322647.1| folylpolyglutamate synthase [Populus trichoc...   375   e-101
ref|XP_002308003.1| folylpolyglutamate synthase [Populus trichoc...   375   e-101
ref|XP_004136673.1| PREDICTED: folylpolyglutamate synthase-like ...   360   7e-97

>emb|CBI30065.3| unnamed protein product [Vitis vinifera]
          Length = 673

 Score =  391 bits (1004), Expect = e-106
 Identities = 207/367 (56%), Positives = 258/367 (70%), Gaps = 3/367 (0%)
 Frame = +2

Query: 140  GDTLGQIASEKAGILKSGVPAYTVPQLSEAMSVLQDRAATLMVHLDIALPLQQKMLRGLK 319
            GDTLG+IAS KAGI K  +PA+TVPQLSEAM VLQ RA  L V L++A PL  K L+GLK
Sbjct: 307  GDTLGKIASHKAGIFKPQIPAFTVPQLSEAMDVLQQRAHELEVPLEVAAPLDHKNLKGLK 366

Query: 320  LGLDGLHQFTNASLAVALCRCWLEQTKHQTFFLDD-NLDAGLPEPFLQGLCAARLQGRAQ 496
            L L G HQF NA LAV+LC+CWL+ T +    L + N +  L E FL+GL  ARL GRAQ
Sbjct: 367  LSLSGDHQFINAGLAVSLCKCWLQSTGNWEKLLKNANQEDDLLEAFLRGLSTARLSGRAQ 426

Query: 497  IVHDIPEKSLQMPDIDECS-SNLVFYLDGAHSPESMEICANWFSDSVTGEKNSLKNNLMD 673
             V+D P KS  + +  E S  +L+FYLDGAHSPESM++CA WFS++V   +NS  ++ + 
Sbjct: 427  TVYDTPLKSYNLSEATENSFGDLIFYLDGAHSPESMDVCARWFSNAVKERRNSPSSSFVK 486

Query: 674  EFNPLNTVANGKMCYPNRSNKISKRILLFNCMEARDPELLLPQLFKICAGKGIHFSKALF 853
              N +N   + K      SNKISK+ILLFNCME RDP +LLPQL + CA  G HFSKALF
Sbjct: 487  VGNMVNGYIHHKKEDTEESNKISKQILLFNCMEVRDPHILLPQLVRTCASSGTHFSKALF 546

Query: 854  VPSMSIYHKVDPGLSDRTLE-TSMDTSWQSTLQRTWEKMIHGRDNIPENDSKLQKPDNLP 1030
            VPS+S Y+KV  G S   L+  + D SWQ  LQR WEK+IH +D + +   K+  P  LP
Sbjct: 547  VPSISTYNKVTSGDSIVPLDLPARDLSWQFNLQRIWEKIIHNKDVVVDESFKMDSPRILP 606

Query: 1031 SYDFLHGSSENCNPPGKNVTCSAVIPSLPLTIKWLRDCVKENPSFRLQVLVTGSLHLVGD 1210
             ++FL+ +  + +P  K ++ SAV+PSLPLTI+WLRDCVKENPS RLQVLVTGSLHLVGD
Sbjct: 607  PFEFLYENGSSGSPSSKCLSSSAVMPSLPLTIRWLRDCVKENPSLRLQVLVTGSLHLVGD 666

Query: 1211 VLKLIHR 1231
            VLKL+ R
Sbjct: 667  VLKLLRR 673


>ref|XP_002274685.1| PREDICTED: folylpolyglutamate synthase, mitochondrial [Vitis
            vinifera]
          Length = 570

 Score =  391 bits (1004), Expect = e-106
 Identities = 207/367 (56%), Positives = 258/367 (70%), Gaps = 3/367 (0%)
 Frame = +2

Query: 140  GDTLGQIASEKAGILKSGVPAYTVPQLSEAMSVLQDRAATLMVHLDIALPLQQKMLRGLK 319
            GDTLG+IAS KAGI K  +PA+TVPQLSEAM VLQ RA  L V L++A PL  K L+GLK
Sbjct: 204  GDTLGKIASHKAGIFKPQIPAFTVPQLSEAMDVLQQRAHELEVPLEVAAPLDHKNLKGLK 263

Query: 320  LGLDGLHQFTNASLAVALCRCWLEQTKHQTFFLDD-NLDAGLPEPFLQGLCAARLQGRAQ 496
            L L G HQF NA LAV+LC+CWL+ T +    L + N +  L E FL+GL  ARL GRAQ
Sbjct: 264  LSLSGDHQFINAGLAVSLCKCWLQSTGNWEKLLKNANQEDDLLEAFLRGLSTARLSGRAQ 323

Query: 497  IVHDIPEKSLQMPDIDECS-SNLVFYLDGAHSPESMEICANWFSDSVTGEKNSLKNNLMD 673
             V+D P KS  + +  E S  +L+FYLDGAHSPESM++CA WFS++V   +NS  ++ + 
Sbjct: 324  TVYDTPLKSYNLSEATENSFGDLIFYLDGAHSPESMDVCARWFSNAVKERRNSPSSSFVK 383

Query: 674  EFNPLNTVANGKMCYPNRSNKISKRILLFNCMEARDPELLLPQLFKICAGKGIHFSKALF 853
              N +N   + K      SNKISK+ILLFNCME RDP +LLPQL + CA  G HFSKALF
Sbjct: 384  VGNMVNGYIHHKKEDTEESNKISKQILLFNCMEVRDPHILLPQLVRTCASSGTHFSKALF 443

Query: 854  VPSMSIYHKVDPGLSDRTLE-TSMDTSWQSTLQRTWEKMIHGRDNIPENDSKLQKPDNLP 1030
            VPS+S Y+KV  G S   L+  + D SWQ  LQR WEK+IH +D + +   K+  P  LP
Sbjct: 444  VPSISTYNKVTSGDSIVPLDLPARDLSWQFNLQRIWEKIIHNKDVVVDESFKMDSPRILP 503

Query: 1031 SYDFLHGSSENCNPPGKNVTCSAVIPSLPLTIKWLRDCVKENPSFRLQVLVTGSLHLVGD 1210
             ++FL+ +  + +P  K ++ SAV+PSLPLTI+WLRDCVKENPS RLQVLVTGSLHLVGD
Sbjct: 504  PFEFLYENGSSGSPSSKCLSSSAVMPSLPLTIRWLRDCVKENPSLRLQVLVTGSLHLVGD 563

Query: 1211 VLKLIHR 1231
            VLKL+ R
Sbjct: 564  VLKLLRR 570


>ref|XP_002322647.1| folylpolyglutamate synthase [Populus trichocarpa]
            gi|222867277|gb|EEF04408.1| folylpolyglutamate synthase
            [Populus trichocarpa]
          Length = 581

 Score =  375 bits (962), Expect = e-101
 Identities = 203/375 (54%), Positives = 255/375 (68%), Gaps = 11/375 (2%)
 Frame = +2

Query: 140  GDTLGQIASEKAGILKSGVPAYTVPQLSEAMSVLQDRAATLMVHLDIALPLQQKMLRGLK 319
            G+T+GQIAS KAG+ K  +PA+TV Q+SEAM VLQ+ A  LMV L +  PL  K L GLK
Sbjct: 207  GNTIGQIASHKAGVFKHQIPAFTVLQVSEAMDVLQENARELMVPLKVVEPLDSKALNGLK 266

Query: 320  LGLDGLHQFTNASLAVALCRCWLEQTKH-QTFFLDDNLDAGLPEPFLQGLCAARLQGRAQ 496
            L L G HQF+NA LAV+LC+ WL++T + +  F  DN +A LPE FL+GL  A + GRAQ
Sbjct: 267  LSLSGDHQFSNAGLAVSLCKSWLQRTGNWEKLFQKDNREANLPEAFLRGLSTAHISGRAQ 326

Query: 497  IVHDIPEKSLQMP-DIDECSSNLVFYLDGAHSPESMEICANWFSDSVTGEKNSLKNNLMD 673
            IVHD    S  +  ++ E S +L+FYLDGAHSPES+E CA WFS +V G   S    L  
Sbjct: 327  IVHDSSSSSSHVSSEVAETSGDLIFYLDGAHSPESIEACAKWFSVAVKGNNQSPPLVLSS 386

Query: 674  EFN--PLNTV-ANGKM----CYPNRSNKISKRILLFNCMEARDPELLLPQLFKICAGKGI 832
              N   +N V  NG +    C     NKISK+ILLFNCME RDP++LLP+L   CA  G 
Sbjct: 387  SHNIENINIVWKNGHVQHEKCNIQEFNKISKKILLFNCMEVRDPQILLPRLVSTCASSGT 446

Query: 833  HFSKALFVPSMSIYHKVDPGLSDRTLE-TSMDTSWQSTLQRTWEKMIHGRD-NIPENDSK 1006
             FSKA+FVPS+S Y+KV  G S    + +S D SWQ +LQR WE+++HG D ++ E  +K
Sbjct: 447  FFSKAIFVPSISTYNKVTSGTSVIPSDISSKDLSWQFSLQRLWERIVHGIDTDLLEKSTK 506

Query: 1007 LQKPDNLPSYDFLHGSSENCNPPGKNVTCSAVIPSLPLTIKWLRDCVKENPSFRLQVLVT 1186
            +   +  P  +FL+  + NC+P    + CSAVIPSLPLTIKWLRDCV+ENPS RLQVLVT
Sbjct: 507  MDAAETSPHREFLYEDASNCSPSNGYLACSAVIPSLPLTIKWLRDCVRENPSLRLQVLVT 566

Query: 1187 GSLHLVGDVLKLIHR 1231
            GSLHLVGDVLK+I R
Sbjct: 567  GSLHLVGDVLKVIRR 581


>ref|XP_002308003.1| folylpolyglutamate synthase [Populus trichocarpa]
            gi|222853979|gb|EEE91526.1| folylpolyglutamate synthase
            [Populus trichocarpa]
          Length = 588

 Score =  375 bits (962), Expect = e-101
 Identities = 203/379 (53%), Positives = 260/379 (68%), Gaps = 15/379 (3%)
 Frame = +2

Query: 140  GDTLGQIASEKAGILKSGVPAYTVPQLSEAMSVLQDRAATLMVHLDIALPLQQKMLRGLK 319
            G+T+GQIAS KAGI K  +PA+TVPQ+SEAM VLQ+ A  L V L +  PL    L GLK
Sbjct: 212  GNTIGQIASHKAGIFKHQIPAFTVPQVSEAMDVLQENAQELTVPLKVVEPLDSNELNGLK 271

Query: 320  LGLDGLHQFTNASLAVALCRCWLEQTKH-QTFFLDDNLDAGLPEPFLQGLCAARLQGRAQ 496
            L L G HQ +NA LAV+LC+CWL++T + +  F  DN +A LPE FL+GL  A + GRAQ
Sbjct: 272  LSLSGNHQLSNAGLAVSLCKCWLQRTGNWEKLFQIDNKEANLPEAFLRGLSKAHIAGRAQ 331

Query: 497  IVHDIPEKSLQM--PDIDECSSNLVFYLDGAHSPESMEICANWFSDSVTGEKNSLKNNLM 670
            IV D+   S      ++ E S +L+FYLDGAHSPESME+CA WFS +V  ++N+L ++L+
Sbjct: 332  IVPDLSSTSSSCISSEVAEASGDLIFYLDGAHSPESMEVCAEWFSSAV--KENNLSSSLV 389

Query: 671  D----EFNPLNTV-ANGKMCYPNRS----NKISKRILLFNCMEARDPELLLPQLFKICAG 823
                 +   +N V  NG M +   +    NKISK+ILLFNCM+ RDP++LLP+L   CA 
Sbjct: 390  SFSSHDIESINKVPGNGYMQHEKFNIQEINKISKKILLFNCMDVRDPQILLPRLVSTCAS 449

Query: 824  KGIHFSKALFVPSMSIYHKVDPGLSDRTLE-TSMDTSWQSTLQRTWEKMIHG--RDNIPE 994
             G  FSKA+FVPS+S Y+KV  G S    + +S D SWQ +LQR WEK++HG   D++ E
Sbjct: 450  SGTFFSKAIFVPSISTYNKVTSGTSVVPSDISSKDLSWQFSLQRLWEKIVHGIDTDSLLE 509

Query: 995  NDSKLQKPDNLPSYDFLHGSSENCNPPGKNVTCSAVIPSLPLTIKWLRDCVKENPSFRLQ 1174
              +K+   + LP   FL+  + NC+P    + CSAVIPSLPLTIKWLR CV+ENPS RLQ
Sbjct: 510  KSTKMDGAETLPRRQFLYEDASNCSPTDGYLACSAVIPSLPLTIKWLRHCVRENPSLRLQ 569

Query: 1175 VLVTGSLHLVGDVLKLIHR 1231
            VLVTGSLHLVGDVLKLI R
Sbjct: 570  VLVTGSLHLVGDVLKLIRR 588


>ref|XP_004136673.1| PREDICTED: folylpolyglutamate synthase-like [Cucumis sativus]
          Length = 558

 Score =  360 bits (923), Expect = 7e-97
 Identities = 205/376 (54%), Positives = 247/376 (65%), Gaps = 12/376 (3%)
 Frame = +2

Query: 140  GDTLGQIASEKAGILKSGVPAYTVPQLSEAMSVLQDRAATLMVHLDIALPLQQKMLRGLK 319
            GD+LG IAS KAGILK  VPA+TVPQL EAM V+Q +A  LMV L++A PL  K L GLK
Sbjct: 195  GDSLGMIASHKAGILKPQVPAFTVPQLPEAMDVIQKKARELMVPLEVAEPLNDKKLDGLK 254

Query: 320  LGLDGLHQFTNASLAVALCRCWLEQTKH-QTFFLDDNLDAGLPEPFLQGLCAARLQGRAQ 496
            LG+ G HQF N+ LAV+LC+CWL++T + +   L+    A +P  FL+GL  A L GRAQ
Sbjct: 255  LGMSGDHQFVNSGLAVSLCKCWLQRTGNWERMLLNGCKGAKIPAAFLRGLSTANLSGRAQ 314

Query: 497  IVHDIPEKSL-QMPDIDECSSNLVFYLDGAHSPESMEICANWFSDSVTGEKNSL-----K 658
             V+D    S       D  + +LVFYLDGAHSPESME CA+WFS  V G   SL     K
Sbjct: 315  TVYDYYTSSFNSTTQSDNSNGDLVFYLDGAHSPESMEACASWFSSVVKGNHKSLNSFRAK 374

Query: 659  NNLMDEFN--PLNTVANGKMCYPNRSNKISKRILLFNCMEARDPELLLPQLFKICAGKGI 832
            N   D  N  P+      K+     S K+SK++LLFNCME RDP +LLP+L   CA  G+
Sbjct: 375  NVDRDSGNDDPV------KVLQEAESTKLSKQVLLFNCMEVRDPHILLPRLVNTCASSGV 428

Query: 833  HFSKALFVPSMSIYHKVDPGLSDRTLET-SMDTSWQSTLQRTWEKMIHGRDNIPENDSKL 1009
            HFSKALFVP+MS Y KV  G S  + +  S D SWQ  LQR WEK++HG+D I E D KL
Sbjct: 429  HFSKALFVPTMSTYSKVSSGNSVTSSDNISKDLSWQLNLQRLWEKIMHGKDAILEKDLKL 488

Query: 1010 QKPDNLPSY--DFLHGSSENCNPPGKNVTCSAVIPSLPLTIKWLRDCVKENPSFRLQVLV 1183
                 LP+   D L   SEN      N++ SAV+PSLPL IKWLRDCV+E+PS RLQVLV
Sbjct: 489  DSERGLPALYEDSLSSLSEN------NLSSSAVMPSLPLAIKWLRDCVRESPSIRLQVLV 542

Query: 1184 TGSLHLVGDVLKLIHR 1231
            TGSLHLVGDVLKL+ R
Sbjct: 543  TGSLHLVGDVLKLLKR 558