BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dioscorea21_contig00014158
         (1812 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002268388.1| PREDICTED: nitric oxide synthase 1 [Vitis vi...   721   0.0  
ref|XP_004158109.1| PREDICTED: NO-associated protein 1, chloropl...   717   0.0  
ref|XP_002510962.1| Nitric-oxide synthase, putative [Ricinus com...   715   0.0  
ref|XP_004143656.1| PREDICTED: NO-associated protein 1, chloropl...   714   0.0  
dbj|BAF93184.1| nitric oxide associated 1 [Nicotiana benthamiana]     699   0.0  

>ref|XP_002268388.1| PREDICTED: nitric oxide synthase 1 [Vitis vinifera]
            gi|296084222|emb|CBI24610.3| unnamed protein product
            [Vitis vinifera]
          Length = 564

 Score =  721 bits (1861), Expect = 0.0
 Identities = 367/550 (66%), Positives = 422/550 (76%), Gaps = 9/550 (1%)
 Frame = +3

Query: 81   PIFPKTLTPRSVS------PFSCRXXXXXXXXXXXXXXXXXXXGTGAAAPTRGDLFLERQ 242
            P  P   TP+  S      P SC+                   G GAAAPTRGDLFLE  
Sbjct: 17   PYSPSNPTPKFSSFYTKPTPISCQTQAHQQAAPTSDPYRPESDGLGAAAPTRGDLFLEHH 76

Query: 243  QSRSAATVVLXXXXXXXXXXXXXXXXLSSAIACCYGCGAPLQTEEMEAPGYVDPETYELK 422
            QS +A+ VV                  S+A AC YGCGAPLQT E +APGYVDPETYELK
Sbjct: 77   QSVAASEVVFNANKKKKKVKFSGSWKASAASAC-YGCGAPLQTLETDAPGYVDPETYELK 135

Query: 423  KKHRQLRTVLCGRCKLLSHGHMVTAVGGHGGYPGGKQFVTAEELREKLSHLRHEKVLIVK 602
            KKHRQLRTVLCGRC+LLSHG M+TAVGG+GGY GGKQF++AEELREKLSHLRHEK LIVK
Sbjct: 136  KKHRQLRTVLCGRCRLLSHGQMITAVGGNGGYSGGKQFISAEELREKLSHLRHEKALIVK 195

Query: 603  LVDIVDFNGSFLSRIRDLAGANPIILVVTKVDLLPKGTELNCIGDWVVDATIKKKLNVIS 782
            LVDIVDFNGSFL+ +RDLAGANPIILVVTKVDLLPK T+LNC+GDWVV+AT+KKKLNV+S
Sbjct: 196  LVDIVDFNGSFLAHVRDLAGANPIILVVTKVDLLPKETDLNCVGDWVVEATMKKKLNVLS 255

Query: 783  VHLTSSKSLVGIVGVIAEIQKEKKGRDVYILGSANVGKSAFINALLKMMGEKDPVAAAAQ 962
            VHLTSSKSLVGI GV +EIQKEKKGR+VYILGSANVGKSAFINALLKMM ++DP AAAAQ
Sbjct: 256  VHLTSSKSLVGISGVASEIQKEKKGRNVYILGSANVGKSAFINALLKMMAQRDPAAAAAQ 315

Query: 963  KYKPIQSAVPGTTLGPIQIEAFQGGGKLFDTPGVHLHHRQAAVVDSEDLPSLAPKNRLRA 1142
            +YKPIQSAVPGTTLGPIQI+AF GGGKL+DTPGVHLHHRQAAVV SEDLP+LAP++RLR 
Sbjct: 316  RYKPIQSAVPGTTLGPIQIDAFLGGGKLYDTPGVHLHHRQAAVVHSEDLPALAPRSRLRG 375

Query: 1143 QSFP---VLSENKRLNDTAALGLNGFSIFWGGLVRVDIIKVLPETRLTFYGPRQLPIHMV 1313
            Q FP   V  ++  L+   + GLNGFSIFWGGLVR+DI+KVLP+TRLTFYGP+ L IHMV
Sbjct: 376  QCFPNFQVAFDDSTLSRIKSNGLNGFSIFWGGLVRIDIVKVLPQTRLTFYGPKALNIHMV 435

Query: 1314 PTSEADQFYHRELGISLTPPTGNQRAEAWPGLQVVRQLQINFENRQRPACDIAISGLGWI 1493
            PT +AD+FY +ELG+ LTPPTG QRAE W GL+  RQLQI FE+  RPACD+AISGLGWI
Sbjct: 436  PTDKADEFYQKELGVLLTPPTGKQRAEDWLGLETERQLQIKFEDSDRPACDLAISGLGWI 495

Query: 1494 AIEPFSSHLNADPNGNNEADLKELHLAVHVPKPVEVFVRPPMPVGKAGXXXXXXXXXXXX 1673
            A+EP    L    + + E   ++L L++ VPKPVE+FVRPP+PVGK G            
Sbjct: 496  AVEPIGRSLRTS-DSDLEETAEQLQLSIQVPKPVEIFVRPPIPVGKGGGEWYQYRELTEK 554

Query: 1674 XXXLRPKWFF 1703
               +RP+W+F
Sbjct: 555  EVEVRPQWYF 564


>ref|XP_004158109.1| PREDICTED: NO-associated protein 1, chloroplastic/mitochondrial-like
            [Cucumis sativus]
          Length = 555

 Score =  717 bits (1850), Expect = 0.0
 Identities = 359/508 (70%), Positives = 413/508 (81%), Gaps = 4/508 (0%)
 Frame = +3

Query: 192  GTGAAAPTRGDLFLERQQSRSAATVVLXXXXXXXXXXXXXXXXLSSAIACCYGCGAPLQT 371
            GTGAAAPTRGD FLER  S  AA  VL                +S A+A CYGCGAPLQT
Sbjct: 56   GTGAAAPTRGDRFLERHHSVEAAKFVLKENNKDRKRKKDKALKVSLAVASCYGCGAPLQT 115

Query: 372  EEMEAPGYVDPETYELKKKHRQLRTVLCGRCKLLSHGHMVTAVGGHGGYPGGKQFVTAEE 551
             E++APGYV+PETYELKKKH QLR V+CGRC+LLSHGHM+TAVGG+GGY GGKQF++AEE
Sbjct: 116  LELDAPGYVEPETYELKKKHHQLRRVICGRCRLLSHGHMITAVGGNGGYSGGKQFISAEE 175

Query: 552  LREKLSHLRHEKVLIVKLVDIVDFNGSFLSRIRDLAGANPIILVVTKVDLLPKGTELNCI 731
            LR+KLSHLR+EK LIVKLVDIVDFNGSFL+R+RDLAGANPIILVVTKVDLLPKGT+LNCI
Sbjct: 176  LRDKLSHLRYEKALIVKLVDIVDFNGSFLARVRDLAGANPIILVVTKVDLLPKGTDLNCI 235

Query: 732  GDWVVDATIKKKLNVISVHLTSSKSLVGIVGVIAEIQKEKKGRDVYILGSANVGKSAFIN 911
            GDWVV+AT KKKL+V+SVHLTSSKSLVG+ GV+A+IQKEKKGRDVYILGSANVGKSAFIN
Sbjct: 236  GDWVVEATTKKKLSVLSVHLTSSKSLVGVAGVVADIQKEKKGRDVYILGSANVGKSAFIN 295

Query: 912  ALLKMMGEKDPVAAAAQKYKPIQSAVPGTTLGPIQIEAFQGGGKLFDTPGVHLHHRQAAV 1091
            A+LK M E+DPVAAAAQKY+PIQSAVPGTTLGPIQI+AF GGGKL+DTPGVHLHHR AAV
Sbjct: 296  AVLKKMAERDPVAAAAQKYRPIQSAVPGTTLGPIQIDAFLGGGKLYDTPGVHLHHRLAAV 355

Query: 1092 VDSEDLPSLAPKNRLRAQSFPVLSENKRLNDTAALGLNGFSIFWGGLVRVDIIKVLPETR 1271
            V SEDLP+LAP++RLR Q F V  ++  +N+  + GLNGFSIFWGGLVRVD++KV PET 
Sbjct: 356  VHSEDLPALAPQSRLRGQHFQV--QHLPMNNELSSGLNGFSIFWGGLVRVDLLKVQPETC 413

Query: 1272 LTFYGPRQLPIHMVPTSEADQFYHRELGISLTPPTGNQRAEAWPGLQVVRQLQINFENRQ 1451
            LTFYGP+ L I +VPT +AD+FY +ELGISLTPP+G Q    W GL+ VRQL+INFE+ Q
Sbjct: 414  LTFYGPKGLQIRVVPTEKADEFYEKELGISLTPPSGKQTRNDWRGLETVRQLKINFEDEQ 473

Query: 1452 RPACDIAISGLGWIAIEPFSSHLNADPNGNNEADLK----ELHLAVHVPKPVEVFVRPPM 1619
            RPACD+AISGLGWI +E   S        NN +DL+    EL LAVHVPKPVE+FVRPPM
Sbjct: 474  RPACDVAISGLGWITVEQKKS------LSNNHSDLESSDDELELAVHVPKPVEIFVRPPM 527

Query: 1620 PVGKAGXXXXXXXXXXXXXXXLRPKWFF 1703
            PVGKAG               +RP+W+F
Sbjct: 528  PVGKAGSEWYQYRDLTEEEEEVRPRWYF 555


>ref|XP_002510962.1| Nitric-oxide synthase, putative [Ricinus communis]
            gi|223550077|gb|EEF51564.1| Nitric-oxide synthase,
            putative [Ricinus communis]
          Length = 567

 Score =  715 bits (1846), Expect = 0.0
 Identities = 371/570 (65%), Positives = 427/570 (74%), Gaps = 12/570 (2%)
 Frame = +3

Query: 30   MAPNPLL-----LSSSHTLCHFPIFPKTLTPRSVSPFSCRXXXXXXXXXXXXXXXXXXX- 191
            MAP  L+     +S  H L HF         R  +P  C+                    
Sbjct: 1    MAPKTLVTFFSPVSLPHNLTHFNTKFLKFNSRKTTPIFCKSTQSQQKPVSDTQLPLSIPE 60

Query: 192  --GTGAAAPTRGDLFLERQQSRSAATVVLXXXXXXXXXXXXXXXXL--SSAIACCYGCGA 359
              GTGAAAPT G+ FLERQQ+  AA +V+                L  +S + CCYGCGA
Sbjct: 61   TSGTGAAAPTPGEQFLERQQAFQAAKLVMKEAKKKRRREKVREKALKVNSMVVCCYGCGA 120

Query: 360  PLQTEEMEAPGYVDPETYELKKKHRQLRTVLCGRCKLLSHGHMVTAVGGHGGYPGGKQFV 539
            PLQT + EAPGYV P+TYELKK+H QLRTVLCGRCKLLSHGHM+TAVGG+GGY GGKQFV
Sbjct: 121  PLQTLDQEAPGYVQPDTYELKKRHHQLRTVLCGRCKLLSHGHMITAVGGNGGYSGGKQFV 180

Query: 540  TAEELREKLSHLRHEKVLIVKLVDIVDFNGSFLSRIRDLAGANPIILVVTKVDLLPKGTE 719
            +A+ELREKLSHLR+E+VL+VKLVDIVDFNGSFL+RIRDLAGANPIILVVTKVDLLPK T+
Sbjct: 181  SADELREKLSHLRYERVLVVKLVDIVDFNGSFLARIRDLAGANPIILVVTKVDLLPKETD 240

Query: 720  LNCIGDWVVDATIKKKLNVISVHLTSSKSLVGIVGVIAEIQKEKKGRDVYILGSANVGKS 899
             NCIGDWVV+AT KKKLNV+SVHLTSSKSLVGI GV++EIQKEKKGRDVYILGSANVGKS
Sbjct: 241  FNCIGDWVVEATTKKKLNVLSVHLTSSKSLVGIAGVVSEIQKEKKGRDVYILGSANVGKS 300

Query: 900  AFINALLKMMGEKDPVAAAAQKYKPIQSAVPGTTLGPIQIEAFQGGGKLFDTPGVHLHHR 1079
            AFINALLKMM ++DP AAAA+KY+PIQSAVPGTTLG IQI+AF GGGKLFDTPGVHLHHR
Sbjct: 301  AFINALLKMMAQRDPAAAAARKYRPIQSAVPGTTLGSIQIDAFLGGGKLFDTPGVHLHHR 360

Query: 1080 QAAVVDSEDLPSLAPKNRLRAQSFPVLSENKRLNDTAALGLNGFSIFWGGLVRVDIIKVL 1259
            QAAVV SEDLP LAP++RLR QSFPV S   + +   + GLNGFSIFWGGLVR+D++KVL
Sbjct: 361  QAAVVQSEDLPILAPRSRLRGQSFPVCS--LQTDKFESNGLNGFSIFWGGLVRIDVLKVL 418

Query: 1260 PETRLTFYGPRQLPIHMVPTSEADQFYH--RELGISLTPPTGNQRAEAWPGLQVVRQLQI 1433
            PET LTFYGP+ L  H+VPT EAD+FY   +ELG+ LTPPTG + A+ W GL ++RQLQI
Sbjct: 419  PETSLTFYGPKALQFHIVPTDEADEFYQASKELGVLLTPPTGIEGAKDWKGLGIIRQLQI 478

Query: 1434 NFENRQRPACDIAISGLGWIAIEPFSSHLNADPNGNNEADLKELHLAVHVPKPVEVFVRP 1613
             FE+ +RPA D+AISGLGWIA+EP S      P  N E   KELHLAVHVP PVE+FVRP
Sbjct: 479  KFEDAERPASDVAISGLGWIAVEPVSKS-RRKPVLNLEETAKELHLAVHVPNPVEIFVRP 537

Query: 1614 PMPVGKAGXXXXXXXXXXXXXXXLRPKWFF 1703
            P+PVGK+G               +RPKW F
Sbjct: 538  PLPVGKSGSEWYQYRELTEKEEEIRPKWHF 567


>ref|XP_004143656.1| PREDICTED: NO-associated protein 1, chloroplastic/mitochondrial-like
            [Cucumis sativus]
          Length = 555

 Score =  714 bits (1844), Expect = 0.0
 Identities = 355/504 (70%), Positives = 413/504 (81%)
 Frame = +3

Query: 192  GTGAAAPTRGDLFLERQQSRSAATVVLXXXXXXXXXXXXXXXXLSSAIACCYGCGAPLQT 371
            GTGAAAPTRGD FLER  S  AA  VL                +S A+A CYGCGAPLQT
Sbjct: 56   GTGAAAPTRGDRFLERHHSVEAAKFVLKENNKDRKRKKDKALKVSLAVASCYGCGAPLQT 115

Query: 372  EEMEAPGYVDPETYELKKKHRQLRTVLCGRCKLLSHGHMVTAVGGHGGYPGGKQFVTAEE 551
             E++APGYV+PETYELKKKH QLR V+CGRC+LLSHGHM+TAVGG+GGY GGKQF++AEE
Sbjct: 116  LELDAPGYVEPETYELKKKHHQLRRVICGRCRLLSHGHMITAVGGNGGYSGGKQFISAEE 175

Query: 552  LREKLSHLRHEKVLIVKLVDIVDFNGSFLSRIRDLAGANPIILVVTKVDLLPKGTELNCI 731
            LR+KLSHLR+EK LIVKLVDIVDFNGSFL+R+RDLAGANPIILVVTKVDLLPKGT+LNCI
Sbjct: 176  LRDKLSHLRYEKALIVKLVDIVDFNGSFLARVRDLAGANPIILVVTKVDLLPKGTDLNCI 235

Query: 732  GDWVVDATIKKKLNVISVHLTSSKSLVGIVGVIAEIQKEKKGRDVYILGSANVGKSAFIN 911
            GDWVV+AT +KKL+V+SVHLTSSKSLVG+ GV+A+IQKEKKGRDVYILGSANVGKSAFIN
Sbjct: 236  GDWVVEATTRKKLSVLSVHLTSSKSLVGVAGVVADIQKEKKGRDVYILGSANVGKSAFIN 295

Query: 912  ALLKMMGEKDPVAAAAQKYKPIQSAVPGTTLGPIQIEAFQGGGKLFDTPGVHLHHRQAAV 1091
            A+LK M E+DPVAAAAQKY+PIQSAVPGTTLGPIQI+AF GGGKL+DTPGVHLHHR AAV
Sbjct: 296  AVLKKMAERDPVAAAAQKYRPIQSAVPGTTLGPIQIDAFLGGGKLYDTPGVHLHHRLAAV 355

Query: 1092 VDSEDLPSLAPKNRLRAQSFPVLSENKRLNDTAALGLNGFSIFWGGLVRVDIIKVLPETR 1271
            V SEDLP+LAP++RLR Q F V  ++  +++  + GLNGFSIFWGGLVRVD++KV PET 
Sbjct: 356  VHSEDLPALAPQSRLRGQHFQV--QHLPMDNELSSGLNGFSIFWGGLVRVDLLKVQPETC 413

Query: 1272 LTFYGPRQLPIHMVPTSEADQFYHRELGISLTPPTGNQRAEAWPGLQVVRQLQINFENRQ 1451
            LTFYGP+ L I +VPT +AD+FY +ELGISLTPP+G Q    W GL+ VRQL+INFE+ Q
Sbjct: 414  LTFYGPKGLQIRVVPTEKADEFYEKELGISLTPPSGKQTRNDWRGLETVRQLKINFEDEQ 473

Query: 1452 RPACDIAISGLGWIAIEPFSSHLNADPNGNNEADLKELHLAVHVPKPVEVFVRPPMPVGK 1631
            RPACD+AISGLGWI +E   S  N   + + E+ + EL LAVHVPKPVE+FVRPPMPVGK
Sbjct: 474  RPACDVAISGLGWITVEQKKSLSN--NHSDLESSVDELELAVHVPKPVEIFVRPPMPVGK 531

Query: 1632 AGXXXXXXXXXXXXXXXLRPKWFF 1703
            AG               +RP+W+F
Sbjct: 532  AGSEWYQYRDLTEEEEEVRPRWYF 555


>dbj|BAF93184.1| nitric oxide associated 1 [Nicotiana benthamiana]
          Length = 568

 Score =  699 bits (1805), Expect = 0.0
 Identities = 345/508 (67%), Positives = 410/508 (80%), Gaps = 4/508 (0%)
 Frame = +3

Query: 192  GTGAAAPTRGDLFLERQQSRSAATVVLXXXXXXXXXXXXXXXXLSSAIACCYGCGAPLQT 371
            G GAAAPTRGD++L+RQQ+ +A++ VL                +S+   CCYGCGAPL T
Sbjct: 64   GYGAAAPTRGDIYLQRQQAVAASSTVLATIKKKKKKKDKIFK-ISNLAPCCYGCGAPLHT 122

Query: 372  EEMEAPGYVDPETYELKKKHRQLRTVLCGRCKLLSHGHMVTAVGGHGGYPGGKQFVTAEE 551
             E++APGYV  ETY+LKKKH QLR VLCGRC+LLSHGHM+TAVGG+GGY GGKQFVTAEE
Sbjct: 123  SEVDAPGYVHQETYDLKKKHHQLRKVLCGRCRLLSHGHMITAVGGNGGYSGGKQFVTAEE 182

Query: 552  LREKLSHLRHEKVLIVKLVDIVDFNGSFLSRIRDLAGANPIILVVTKVDLLPKGTELNCI 731
            LREKLSHLRHEK LIVKLVDIVDFNGSFL+R+RDLAGANPIILV+TKVDLLPK T+LNC+
Sbjct: 183  LREKLSHLRHEKALIVKLVDIVDFNGSFLARVRDLAGANPIILVITKVDLLPKETDLNCV 242

Query: 732  GDWVVDATIKKKLNVISVHLTSSKSLVGIVGVIAEIQKEKKGRDVYILGSANVGKSAFIN 911
            GDWVV+AT+KKKLNV+SVHLTSSKSLVGI GV++EIQKEKKGRDVYILGSANVGKSAFIN
Sbjct: 243  GDWVVEATMKKKLNVLSVHLTSSKSLVGIAGVVSEIQKEKKGRDVYILGSANVGKSAFIN 302

Query: 912  ALLKMMGEKDPVAAAAQKYKPIQSAVPGTTLGPIQIEAFQGGGKLFDTPGVHLHHRQAAV 1091
            ALL  M   DPVAA A+KYKPIQSAVPGTTLGPI I+AF GGGK++DTPGVHLHHRQAAV
Sbjct: 303  ALLNTMSYNDPVAATARKYKPIQSAVPGTTLGPIPIDAFLGGGKVYDTPGVHLHHRQAAV 362

Query: 1092 VDSEDLPSLAPKNRLRAQSFP---VLSENKRLNDTAALGLNGFSIFWGGLVRVDIIKVLP 1262
            + +EDLP+LAP++RLRAQ+FP   +  E++  +   + GLNG SIFWGGLVR+D++KVLP
Sbjct: 363  IHTEDLPTLAPQSRLRAQAFPSSGLKLESQIADRVRSSGLNGLSIFWGGLVRIDVLKVLP 422

Query: 1263 ETRLTFYGPRQLPIHMVPTSEADQFYHRELGISLTPPTGNQRAEAWPGLQVVRQLQINFE 1442
            ET LTFYGP+ L +HMVPT EAD+FY +ELGI LTPPTG ++A+ W GL+  RQLQI +E
Sbjct: 423  ETCLTFYGPKALQLHMVPTEEADEFYQKELGILLTPPTGKEKADGWMGLETKRQLQIKYE 482

Query: 1443 NRQRPACDIAISGLGWIAIEPFSSHL-NADPNGNNEADLKELHLAVHVPKPVEVFVRPPM 1619
            + +RP CD+AISGLGW+++ P +  +  +DP   +E    EL   VHVPKPVE+FVRPP+
Sbjct: 483  DIERPTCDVAISGLGWLSVIPVNKSVGTSDP--VSEVTAGELTFVVHVPKPVEIFVRPPL 540

Query: 1620 PVGKAGXXXXXXXXXXXXXXXLRPKWFF 1703
            PVGKAG               +RPKWFF
Sbjct: 541  PVGKAGGQWYDYRELTEEESEVRPKWFF 568