BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dioscorea21_contig00012213
         (1142 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_003554195.1| PREDICTED: polygalacturonase inhibitor-like ...   188   2e-78
gb|AFW75084.1| polygalacturonase inhibitor [Zea mays]                 172   7e-77
gb|AAR15145.1| polygalacturonase-inhibiting protein [Eucalyptus ...   181   3e-76
gb|AAB19212.1| polygalacturonase-inhibiting protein [Malus x dom...   177   7e-76
ref|NP_001147231.1| polygalacturonase inhibitor precursor [Zea m...   168   1e-75

>ref|XP_003554195.1| PREDICTED: polygalacturonase inhibitor-like [Glycine max]
          Length = 333

 Score =  188 bits (478), Expect(2) = 2e-78
 Identities = 92/139 (66%), Positives = 108/139 (77%)
 Frame = -3

Query: 561 PNSYLLLSHNNLTGPIPRSFQKVDFTRVDLSRNKLTGDASVLFGQSKSTQSIDLSRNKLM 382
           P   L LSHN L+GPIP S   +D  R+D SRNKL GDASVLFG++K+TQ +D+SRN L 
Sbjct: 195 PGPSLWLSHNQLSGPIPASLANIDPQRIDFSRNKLEGDASVLFGRNKTTQIVDVSRNSLA 254

Query: 381 FDLSNVKFPEAELISLELNHNMIYGSIPKQITKVPNLQGFNVSYNRLCGEIPRGGKLGQF 202
           FDLS V+FP + LISL+LNHN I GSIP  +T V  LQGFNVSYNRLCGEIP+GG+L +F
Sbjct: 255 FDLSKVEFPTS-LISLDLNHNQITGSIPVGLTAVDFLQGFNVSYNRLCGEIPQGGRLQKF 313

Query: 201 DKYSFFHNKCLCGPPLAPC 145
           D YS+FHNKCLCG PL  C
Sbjct: 314 DVYSYFHNKCLCGSPLTSC 332



 Score =  131 bits (330), Expect(2) = 2e-78
 Identities = 73/152 (48%), Positives = 95/152 (62%), Gaps = 9/152 (5%)
 Frame = -1

Query: 1064 LCNEQDKQALLIIRDSFGSSNP----PWHSDIACCDWPGIDCDAEGH-VTGMAIL----E 912
            LCN QDK+ LL I+  F  +NP     W+ +  CC+W  + C  E H +  + IL    +
Sbjct: 24   LCNPQDKKVLLQIKKDF--NNPYLLASWNPNTDCCNWYCVQCHPETHRINSLVILSSVPQ 81

Query: 911  NPLVGPIPSAIGDLHYLQVVRLHKLPNLYGTIPPEITKLSKLRFLTISWTGIYGSVPAFL 732
              L GPIP ++GDL +L+ ++ HKLP L G I P I KL+KL+ + ISWT + G VP FL
Sbjct: 82   TNLSGPIPPSVGDLPFLETLQFHKLPKLTGPIQPTIAKLTKLKEIYISWTNVSGPVPDFL 141

Query: 731  AKLTALEQLDLSFNKLSGFIPSSLGDLPKLYS 636
            A+LT L+ LDLSFN LSG IPSSL  L  L S
Sbjct: 142  ARLTNLQFLDLSFNNLSGPIPSSLSQLSNLVS 173


>gb|AFW75084.1| polygalacturonase inhibitor [Zea mays]
          Length = 341

 Score =  172 bits (436), Expect(2) = 7e-77
 Identities = 84/138 (60%), Positives = 101/138 (73%), Gaps = 1/138 (0%)
 Frame = -3

Query: 555 SYLLLSHNNLTGPIPRSFQKVDFTRVDLSRNKLTGDASVLFGQSKSTQSIDLSRNKLMFD 376
           +YL LSHNNLTG +P  F  V F +VDLSRN   GDAS LFG++K  Q +DLSRN   F+
Sbjct: 204 AYLRLSHNNLTGSVPPEFSAVAFAQVDLSRNAFAGDASGLFGRAKPVQQMDLSRNAFSFN 263

Query: 375 LSNVKFPEAELISLELNHNMIYGSIPKQITKVPNLQGFNVSYNRLCGEIPRGGKLGQFDK 196
           LS V+ PE +L SL+L+HN IYG IP Q+  + NLQ FNVSYNRLCG +P GG +  FD 
Sbjct: 264 LSAVELPE-QLNSLDLSHNAIYGGIPAQVVNLTNLQLFNVSYNRLCGAVPTGGNMASFDA 322

Query: 195 YSFFHNKCLCGPPLA-PC 145
           YS+ HN+CLCGPPLA PC
Sbjct: 323 YSYQHNRCLCGPPLADPC 340



 Score =  142 bits (359), Expect(2) = 7e-77
 Identities = 77/148 (52%), Positives = 95/148 (64%), Gaps = 6/148 (4%)
 Frame = -1

Query: 1061 CNEQDKQALLIIRDSFGSSNP----PWHSDIACCDWPGIDCDAE-GHVTGMAILENP-LV 900
            C+  DK ALL I+ + G  NP     W  D  CCDW  +DCDA  G V G+A+ ++  L 
Sbjct: 32   CHSGDKAALLAIKAALG--NPYHFASWTPDNPCCDWYDVDCDATTGRVVGLAVFQDANLT 89

Query: 899  GPIPSAIGDLHYLQVVRLHKLPNLYGTIPPEITKLSKLRFLTISWTGIYGSVPAFLAKLT 720
            G IP A+  L +LQ + LH LP + G IPP I +LS L FLTISWTG+ G VP+FL  LT
Sbjct: 90   GTIPDAVAGLVHLQNLMLHHLPGISGPIPPAIGRLSNLSFLTISWTGVSGPVPSFLGTLT 149

Query: 719  ALEQLDLSFNKLSGFIPSSLGDLPKLYS 636
             L QLDLSFN L+G +P+SL  LP LYS
Sbjct: 150  RLNQLDLSFNALTGAVPASLAALPSLYS 177


>gb|AAR15145.1| polygalacturonase-inhibiting protein [Eucalyptus grandis]
          Length = 331

 Score =  181 bits (459), Expect(2) = 3e-76
 Identities = 88/135 (65%), Positives = 107/135 (79%)
 Frame = -3

Query: 549 LLLSHNNLTGPIPRSFQKVDFTRVDLSRNKLTGDASVLFGQSKSTQSIDLSRNKLMFDLS 370
           L LSHN L+G IP SF ++DFT +DLSRNKL GDASV+FG +K+TQ +DLSRN L F+LS
Sbjct: 197 LYLSHNQLSGNIPTSFAQMDFTSIDLSRNKLEGDASVIFGLNKTTQIVDLSRNLLEFNLS 256

Query: 369 NVKFPEAELISLELNHNMIYGSIPKQITKVPNLQGFNVSYNRLCGEIPRGGKLGQFDKYS 190
            V+FP   L SL++NHN IYGSIP + T++ N Q  NVSYNRLCG+IP GGKL  FD+YS
Sbjct: 257 KVEFP-TSLTSLDINHNKIYGSIPVEFTQL-NFQFLNVSYNRLCGQIPVGGKLQSFDEYS 314

Query: 189 FFHNKCLCGPPLAPC 145
           +FHN+CLCG PL PC
Sbjct: 315 YFHNRCLCGAPLGPC 329



 Score =  131 bits (330), Expect(2) = 3e-76
 Identities = 68/144 (47%), Positives = 87/144 (60%), Gaps = 3/144 (2%)
 Frame = -1

Query: 1064 LCNEQDKQALLIIRDSFGSSN--PPWHSDIACCDWPGIDCDAE-GHVTGMAILENPLVGP 894
            LCN  DK+ LL I+ +FG       W SD  CCDW  + CD+    +  + I    + G 
Sbjct: 26   LCNPDDKKVLLQIKKAFGDPYVLASWKSDTDCCDWYCVTCDSTTNRINSLTIFAGQVSGQ 85

Query: 893  IPSAIGDLHYLQVVRLHKLPNLYGTIPPEITKLSKLRFLTISWTGIYGSVPAFLAKLTAL 714
            IP+ +GDL YL+ +  HK PNL G I P I KL  L+ L +SWT + GSVP FL++L  L
Sbjct: 86   IPALVGDLPYLETLEFHKQPNLTGPIQPAIAKLKGLKSLRLSWTNLSGSVPDFLSQLKNL 145

Query: 713  EQLDLSFNKLSGFIPSSLGDLPKL 642
              LDLSFN L+G IPSSL +LP L
Sbjct: 146  TFLDLSFNNLTGAIPSSLSELPNL 169


>gb|AAB19212.1| polygalacturonase-inhibiting protein [Malus x domestica]
           gi|75753642|gb|ABA26937.1| polygalacturonase-inhibiting
           protein [Malus x domestica]
          Length = 330

 Score =  177 bits (450), Expect(2) = 7e-76
 Identities = 87/135 (64%), Positives = 106/135 (78%)
 Frame = -3

Query: 549 LLLSHNNLTGPIPRSFQKVDFTRVDLSRNKLTGDASVLFGQSKSTQSIDLSRNKLMFDLS 370
           L LSHN L+G IP SF ++DFT +DLSRNKL GDASV+FG +K+TQ +DLSRN L F+LS
Sbjct: 197 LYLSHNQLSGNIPTSFAQMDFTSIDLSRNKLEGDASVIFGLNKTTQIVDLSRNLLEFNLS 256

Query: 369 NVKFPEAELISLELNHNMIYGSIPKQITKVPNLQGFNVSYNRLCGEIPRGGKLGQFDKYS 190
            V+FP   L SL++NHN IYGSIP + T++ N Q  NVSYNRLCG+IP GGKL  FD+YS
Sbjct: 257 KVEFP-TSLTSLDINHNKIYGSIPVEFTQL-NFQFLNVSYNRLCGQIPVGGKLQSFDEYS 314

Query: 189 FFHNKCLCGPPLAPC 145
           +FHN+CLCG PL  C
Sbjct: 315 YFHNRCLCGAPLPSC 329



 Score =  134 bits (336), Expect(2) = 7e-76
 Identities = 69/144 (47%), Positives = 87/144 (60%), Gaps = 3/144 (2%)
 Frame = -1

Query: 1064 LCNEQDKQALLIIRDSFGSSN--PPWHSDIACCDWPGIDCDAE-GHVTGMAILENPLVGP 894
            LCN  DK+ LL I+ +FG       W SD  CCDW  + CD+    +  + I    + G 
Sbjct: 26   LCNPDDKKVLLQIKKAFGDPYVLTSWKSDTDCCDWYCVTCDSTTNRINSLTIFAGQVSGQ 85

Query: 893  IPSAIGDLHYLQVVRLHKLPNLYGTIPPEITKLSKLRFLTISWTGIYGSVPAFLAKLTAL 714
            IP+ +GDL YL+ +  HK PNL G I P I KL  L+FL +SWT + GSVP FL++L  L
Sbjct: 86   IPALVGDLPYLETLEFHKQPNLTGPIQPAIAKLKGLKFLRLSWTNLSGSVPDFLSQLKNL 145

Query: 713  EQLDLSFNKLSGFIPSSLGDLPKL 642
              LDLSFN L+G IPSSL  LP L
Sbjct: 146  TFLDLSFNNLTGAIPSSLSQLPNL 169


>ref|NP_001147231.1| polygalacturonase inhibitor precursor [Zea mays]
           gi|195608848|gb|ACG26254.1| polygalacturonase inhibitor
           precursor [Zea mays]
          Length = 341

 Score =  168 bits (426), Expect(2) = 1e-75
 Identities = 84/138 (60%), Positives = 100/138 (72%), Gaps = 1/138 (0%)
 Frame = -3

Query: 555 SYLLLSHNNLTGPIPRSFQKVDFTRVDLSRNKLTGDASVLFGQSKSTQSIDLSRNKLMFD 376
           +YL LSHNNLTG +P  F  V F +VDLSRN   GDAS LFG++K  Q +DLSRN   F+
Sbjct: 204 AYLRLSHNNLTGSVPPEFSAVAFAQVDLSRNAFAGDASGLFGRAKPVQQMDLSRNAFSFN 263

Query: 375 LSNVKFPEAELISLELNHNMIYGSIPKQITKVPNLQGFNVSYNRLCGEIPRGGKLGQFDK 196
           LS V+ PE +L SL+L+HN IYG IP Q+  + NLQ F VSYNRL G +P GG +G FD 
Sbjct: 264 LSAVELPE-QLNSLDLSHNAIYGGIPGQVVNLTNLQLFKVSYNRLWGAVPPGGNMGSFDA 322

Query: 195 YSFFHNKCLCGPPLA-PC 145
           YSF HN+CLCGPPLA PC
Sbjct: 323 YSFQHNRCLCGPPLADPC 340



 Score =  142 bits (359), Expect(2) = 1e-75
 Identities = 77/148 (52%), Positives = 95/148 (64%), Gaps = 6/148 (4%)
 Frame = -1

Query: 1061 CNEQDKQALLIIRDSFGSSNP----PWHSDIACCDWPGIDCDAE-GHVTGMAILENP-LV 900
            C+  DK ALL I+ + G  NP     W  D  CCDW  +DCDA  G V G+A+ ++  L 
Sbjct: 32   CHSGDKAALLAIKAALG--NPYHFASWTPDNPCCDWYDVDCDATTGRVVGLAVFQDANLT 89

Query: 899  GPIPSAIGDLHYLQVVRLHKLPNLYGTIPPEITKLSKLRFLTISWTGIYGSVPAFLAKLT 720
            G IP A+  L +LQ + LH LP + G IPP I +LS L FLTISWTG+ G VP+FL  LT
Sbjct: 90   GTIPDAVSGLVHLQNLMLHHLPGISGPIPPAIGRLSNLSFLTISWTGVSGPVPSFLGALT 149

Query: 719  ALEQLDLSFNKLSGFIPSSLGDLPKLYS 636
             L QLDLSFN L+G +P+SL  LP LYS
Sbjct: 150  RLNQLDLSFNALTGAVPASLAALPSLYS 177