BLASTX nr result

ID: Dioscorea21_contig00012166 seq

BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dioscorea21_contig00012166
         (377 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ACJ85868.1| unknown [Medicago truncatula] gi|388518991|gb|AFK...   106   2e-21
ref|XP_003611658.1| Isoflavone 2'-hydroxylase [Medicago truncatu...    99   3e-19
gb|AAQ20042.1| CYP81E8 [Medicago truncatula]                           99   3e-19
ref|XP_003548043.1| PREDICTED: isoflavone 2'-hydroxylase-like [G...    98   8e-19
dbj|BAA93634.1| cytochrome P450 [Lotus japonicus]                      97   1e-18

>gb|ACJ85868.1| unknown [Medicago truncatula] gi|388518991|gb|AFK47557.1| unknown
           [Medicago truncatula]
          Length = 509

 Score =  106 bits (264), Expect = 2e-21
 Identities = 56/125 (44%), Positives = 76/125 (60%), Gaps = 4/125 (3%)
 Frame = -1

Query: 368 VRSDEACSLVKALLREYSGPS---FQRTELRPKFFGFAYNAVMRMIANKRYYAY-ADESS 201
           +R +E   L++AL R  S  S   F R EL+ K     +N +MRMI+ KRYY    D S 
Sbjct: 146 IRKNEIMKLMQALARGSSSSSSDGFVRVELKTKLSEMTFNTIMRMISGKRYYGEDCDVSD 205

Query: 200 SEGGNEFRDMVKKTTSIAGVSNAADFIPLARWFRVGGHEKKLQGLKKRRDKFFQGLIDEH 21
            E    FR+M+K+  S+ G SN  +F+ + RWF  GG+EKKL+ + +R D F QGLIDEH
Sbjct: 206 EEEAKGFREMIKEMVSLGGSSNPGEFVGILRWFDFGGYEKKLKRIARRFDGFLQGLIDEH 265

Query: 20  RTKKK 6
           R KK+
Sbjct: 266 RRKKE 270


>ref|XP_003611658.1| Isoflavone 2'-hydroxylase [Medicago truncatula]
           gi|355512993|gb|AES94616.1| Isoflavone 2'-hydroxylase
           [Medicago truncatula]
          Length = 499

 Score = 99.4 bits (246), Expect = 3e-19
 Identities = 52/124 (41%), Positives = 73/124 (58%), Gaps = 2/124 (1%)
 Frame = -1

Query: 371 DVRSDEACSLVKALLRE-YSGPSFQRTELRPKFFGFAYNAVMRMIANKRYYAY-ADESSS 198
           ++R DE   L++ L ++ Y+G  F   ELRP F    +N +MRM++ KRYY    D S  
Sbjct: 146 EIRRDEIMRLIQKLAQKSYNG--FTEVELRPMFSEMTFNTIMRMVSGKRYYGNDCDVSDV 203

Query: 197 EGGNEFRDMVKKTTSIAGVSNAADFIPLARWFRVGGHEKKLQGLKKRRDKFFQGLIDEHR 18
           E    FR ++K+  S+ G +N  DF+   RWF   G EK+L+ + KR D F QGLIDEHR
Sbjct: 204 EEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFDGLEKRLKKISKRTDAFLQGLIDEHR 263

Query: 17  TKKK 6
             K+
Sbjct: 264 FGKR 267


>gb|AAQ20042.1| CYP81E8 [Medicago truncatula]
          Length = 499

 Score = 99.4 bits (246), Expect = 3e-19
 Identities = 52/124 (41%), Positives = 73/124 (58%), Gaps = 2/124 (1%)
 Frame = -1

Query: 371 DVRSDEACSLVKALLRE-YSGPSFQRTELRPKFFGFAYNAVMRMIANKRYYAY-ADESSS 198
           ++R DE   L++ L ++ Y+G  F   ELRP F    +N +MRM++ KRYY    D S  
Sbjct: 146 EIRRDEIMRLIQKLAQKSYNG--FTEVELRPMFSEMTFNTIMRMVSGKRYYGNDCDVSDV 203

Query: 197 EGGNEFRDMVKKTTSIAGVSNAADFIPLARWFRVGGHEKKLQGLKKRRDKFFQGLIDEHR 18
           E    FR ++K+  S+ G +N  DF+   RWF   G EK+L+ + KR D F QGLIDEHR
Sbjct: 204 EEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFDGLEKRLKKISKRTDAFLQGLIDEHR 263

Query: 17  TKKK 6
             K+
Sbjct: 264 FGKR 267


>ref|XP_003548043.1| PREDICTED: isoflavone 2'-hydroxylase-like [Glycine max]
          Length = 498

 Score = 97.8 bits (242), Expect = 8e-19
 Identities = 49/120 (40%), Positives = 72/120 (60%), Gaps = 1/120 (0%)
 Frame = -1

Query: 365 RSDEACSLVKALLREYSGPSFQRTELRPKFFGFAYNAVMRMIANKRYYAY-ADESSSEGG 189
           R DE   LV+ L R+ S   F + EL+ +F    +N +MRM++ KRYY    D S  +  
Sbjct: 145 RRDEIMRLVQKLARD-SRNGFTKVELKSRFSEMTFNTIMRMVSGKRYYGEDCDVSDVQEA 203

Query: 188 NEFRDMVKKTTSIAGVSNAADFIPLARWFRVGGHEKKLQGLKKRRDKFFQGLIDEHRTKK 9
            +FR+++K+  ++ G +N  DF+ L RWF   G EK+L+ + KR D F QGLID+HR  K
Sbjct: 204 RQFREIIKELVTLGGANNPGDFLALLRWFDFDGLEKRLKRISKRTDAFLQGLIDQHRNGK 263


>dbj|BAA93634.1| cytochrome P450 [Lotus japonicus]
          Length = 499

 Score = 97.4 bits (241), Expect = 1e-18
 Identities = 48/124 (38%), Positives = 75/124 (60%), Gaps = 1/124 (0%)
 Frame = -1

Query: 374 SDVRSDEACSLVKALLREYSGPSFQRTELRPKFFGFAYNAVMRMIANKRYYAY-ADESSS 198
           S +R DE   L++ L  + S  +F   EL  +FF   +N +MRMI+ KRYY    D +  
Sbjct: 145 SPIRRDETTRLIRKLAED-SAKNFSEVELTSRFFDMTFNNIMRMISGKRYYGEDCDMTEL 203

Query: 197 EGGNEFRDMVKKTTSIAGVSNAADFIPLARWFRVGGHEKKLQGLKKRRDKFFQGLIDEHR 18
           +  +EFRDMV +   ++G +N ADF+P+ R     G EK+++G+  + D+F +GL+ EHR
Sbjct: 204 QEASEFRDMVTELLQLSGANNKADFMPILRLVDFEGLEKRVKGISSKTDRFLRGLLQEHR 263

Query: 17  TKKK 6
            KK+
Sbjct: 264 DKKQ 267


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