BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dioscorea21_contig00010979
         (1370 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002277399.1| PREDICTED: E3 ubiquitin-protein ligase RHF2A...   197   4e-98
ref|XP_002270570.2| PREDICTED: E3 ubiquitin-protein ligase RHF2A...   198   1e-96
emb|CBI39117.3| unnamed protein product [Vitis vinifera]              198   1e-94
ref|XP_003596933.1| RING finger protein [Medicago truncatula] gi...   194   6e-94
ref|XP_003540274.1| PREDICTED: E3 ubiquitin-protein ligase RHF2A...   196   1e-68

>ref|XP_002277399.1| PREDICTED: E3 ubiquitin-protein ligase RHF2A [Vitis vinifera]
            gi|296084011|emb|CBI24399.3| unnamed protein product
            [Vitis vinifera]
          Length = 393

 Score =  197 bits (500), Expect(2) = 4e-98
 Identities = 93/118 (78%), Positives = 101/118 (85%), Gaps = 1/118 (0%)
 Frame = -3

Query: 1182 MEETKKEGKILSSAAAFVEGGLQDACGDACSICLEDFCDSDPSTVTNCKHEFHLQCILEW 1003
            MEE+ K    L+SAAAFVEGG+Q+AC DACSICLE FCDSDPSTVT+CKHEFHLQCILEW
Sbjct: 6    MEESNKSECHLTSAAAFVEGGIQEACDDACSICLEAFCDSDPSTVTSCKHEFHLQCILEW 65

Query: 1002 CQRSSQCPMCWQALSLKDPISQELLEGVERERNLRASRSRN-TAFYHPALGDFELQHL 832
            CQRSSQCPMCWQ +SLKDP SQELLE VERER  R + SRN T F+HP LGDFELQHL
Sbjct: 66   CQRSSQCPMCWQPISLKDPTSQELLEAVERERTFRFNPSRNATIFHHPTLGDFELQHL 123



 Score =  189 bits (480), Expect(2) = 4e-98
 Identities = 119/272 (43%), Positives = 155/272 (56%), Gaps = 16/272 (5%)
 Frame = -1

Query: 770 LPLGPNDAELEERIFQHLAAAAAMGRAQHIARRDGQRGRSAARGRPQYVVFSTNSSSVPA 591
           LP+G NDAELEERI QHLAAAAAMGRA+HIARR+GQR RS+A+GRPQ++VFS + S+  A
Sbjct: 123 LPVGANDAELEERIIQHLAAAAAMGRARHIARREGQRTRSSAQGRPQFLVFSAHPSTPQA 182

Query: 590 XXXXXXXXXXXXXXXXXXXXXXXXXGNTLEESIPXXXXXXXXXXXXXXXXQPGPSSAATA 411
                                       +  S+P                       A+A
Sbjct: 183 GPASSSPAQRGEDEPAPAITA------AMPSSLPTAVGEESSRLTTSQLSSQAEEITASA 236

Query: 410 RPVLXXXXXXXXXSTQDRAGP-------------SDFQSFSETLKSRFNAVSLRYKESIA 270
              +         S+ +R  P             S+FQSFSETLKSRFNAVS+RYKESI+
Sbjct: 237 SGSIIHTTNQHGTSSNNRRSPGQSLPSGQDKAGPSEFQSFSETLKSRFNAVSMRYKESIS 296

Query: 269 NSTRGWKERFFSRNTSAADLHSDTRRDVNTEVATVSRMMERLGSRNGIQATSPSLAQSTE 90
            STRGWKER FSRNTS AD+ SD RR+VN  +ATVSRMME L +R+  + T  S++ S+E
Sbjct: 297 KSTRGWKERLFSRNTSMADIGSDVRREVNAGIATVSRMMEHLETRDNSRTTPSSVSDSSE 356

Query: 89  DHTYSSV--ERVSDNSA-NPATNGSSAPACFA 3
           D +  S+  +R+++ SA NP  + +   +C A
Sbjct: 357 DTSSPSLGDQRMAETSAHNPLGDTNVQASCAA 388


>ref|XP_002270570.2| PREDICTED: E3 ubiquitin-protein ligase RHF2A-like [Vitis vinifera]
          Length = 387

 Score =  198 bits (504), Expect(2) = 1e-96
 Identities = 89/117 (76%), Positives = 101/117 (86%)
 Frame = -3

Query: 1182 MEETKKEGKILSSAAAFVEGGLQDACGDACSICLEDFCDSDPSTVTNCKHEFHLQCILEW 1003
            MEE K     ++SAAAFVEGG+Q+AC DACSICLE FCDSDPST+T+CKHEFHLQCILEW
Sbjct: 6    MEEVKNSENHMTSAAAFVEGGVQEACDDACSICLEAFCDSDPSTLTSCKHEFHLQCILEW 65

Query: 1002 CQRSSQCPMCWQALSLKDPISQELLEGVERERNLRASRSRNTAFYHPALGDFELQHL 832
            CQRSSQCPMCWQ++SLKDP SQELLE VERERN+R +  R+  F+HP LGDFELQHL
Sbjct: 66   CQRSSQCPMCWQSISLKDPTSQELLEAVERERNIRLTPPRSAIFHHPTLGDFELQHL 122



 Score =  182 bits (463), Expect(2) = 1e-96
 Identities = 120/265 (45%), Positives = 144/265 (54%), Gaps = 9/265 (3%)
 Frame = -1

Query: 770 LPLGPNDAELEERIFQHLAAAAAMGRAQHIARRDGQRGRSAARGRPQYVVFSTN-----S 606
           LP+G ND ELEERI QHLAAAAAMGRA HIARR+GQR RS+A GR  ++VFST+     S
Sbjct: 122 LPVGTNDPELEERIIQHLAAAAAMGRAHHIARREGQRSRSSAHGRSHFLVFSTHPNATAS 181

Query: 605 SSVPAXXXXXXXXXXXXXXXXXXXXXXXXXGNTLEESIPXXXXXXXXXXXXXXXXQPGPS 426
             VPA                         G+   + IP                     
Sbjct: 182 GPVPASLVQVGENEPAAITVASPSIPLPAGGDEPSQEIPQFPSVQTDHMSAS-----ASR 236

Query: 425 SAATARPVLXXXXXXXXXST---QDRAGPSDFQSFSETLKSRFNAVSLRYKESIANSTRG 255
           SA T R +          S+   QDRAGPS+ QSFSE+LKSR NAVS+RYKESI+ STRG
Sbjct: 237 SAVTPRRISFNNRSSASQSSPPDQDRAGPSELQSFSESLKSRLNAVSMRYKESISKSTRG 296

Query: 254 WKERFFSRNTSAADLHSDTRRDVNTEVATVSRMMERLGSRNGIQATSPSLAQSTEDH-TY 78
           WKER FSRN S ADL S+ RR+VN  +A+VSRMMERL +R    A   S++    D  T 
Sbjct: 297 WKERLFSRNNSMADLGSEVRREVNAGIASVSRMMERLDTRENNGAGQASVSSHLADSVTE 356

Query: 77  SSVERVSDNSANPATNGSSAPACFA 3
            S +   +  A    N S+ PA  A
Sbjct: 357 QSNQNERETRAENPLNDSNTPAACA 381


>emb|CBI39117.3| unnamed protein product [Vitis vinifera]
          Length = 391

 Score =  198 bits (504), Expect(2) = 1e-94
 Identities = 89/117 (76%), Positives = 101/117 (86%)
 Frame = -3

Query: 1182 MEETKKEGKILSSAAAFVEGGLQDACGDACSICLEDFCDSDPSTVTNCKHEFHLQCILEW 1003
            MEE K     ++SAAAFVEGG+Q+AC DACSICLE FCDSDPST+T+CKHEFHLQCILEW
Sbjct: 6    MEEVKNSENHMTSAAAFVEGGVQEACDDACSICLEAFCDSDPSTLTSCKHEFHLQCILEW 65

Query: 1002 CQRSSQCPMCWQALSLKDPISQELLEGVERERNLRASRSRNTAFYHPALGDFELQHL 832
            CQRSSQCPMCWQ++SLKDP SQELLE VERERN+R +  R+  F+HP LGDFELQHL
Sbjct: 66   CQRSSQCPMCWQSISLKDPTSQELLEAVERERNIRLTPPRSAIFHHPTLGDFELQHL 122



 Score =  176 bits (446), Expect(2) = 1e-94
 Identities = 111/231 (48%), Positives = 132/231 (57%), Gaps = 8/231 (3%)
 Frame = -1

Query: 770 LPLGPNDAELEERIFQHLAAAAAMGRAQHIARRDGQRGRSAARGRPQYVVFSTN-----S 606
           LP+G ND ELEERI QHLAAAAAMGRA HIARR+GQR RS+A GR  ++VFST+     S
Sbjct: 122 LPVGTNDPELEERIIQHLAAAAAMGRAHHIARREGQRSRSSAHGRSHFLVFSTHPNATAS 181

Query: 605 SSVPAXXXXXXXXXXXXXXXXXXXXXXXXXGNTLEESIPXXXXXXXXXXXXXXXXQPGPS 426
             VPA                         G+   + IP                     
Sbjct: 182 GPVPASLVQVGENEPAAITVASPSIPLPAGGDEPSQEIPQFPSVQTDHMSAS-----ASR 236

Query: 425 SAATARPVLXXXXXXXXXST---QDRAGPSDFQSFSETLKSRFNAVSLRYKESIANSTRG 255
           SA T R +          S+   QDRAGPS+ QSFSE+LKSR NAVS+RYKESI+ STRG
Sbjct: 237 SAVTPRRISFNNRSSASQSSPPDQDRAGPSELQSFSESLKSRLNAVSMRYKESISKSTRG 296

Query: 254 WKERFFSRNTSAADLHSDTRRDVNTEVATVSRMMERLGSRNGIQATSPSLA 102
           WKER FSRN S ADL S+ RR+VN  +A+VSRMMERL +R    A   S++
Sbjct: 297 WKERLFSRNNSMADLGSEVRREVNAGIASVSRMMERLDTRENNGAGQASVS 347


>ref|XP_003596933.1| RING finger protein [Medicago truncatula] gi|355485981|gb|AES67184.1|
            RING finger protein [Medicago truncatula]
          Length = 382

 Score =  194 bits (492), Expect(2) = 6e-94
 Identities = 91/116 (78%), Positives = 102/116 (87%), Gaps = 1/116 (0%)
 Frame = -3

Query: 1176 ETKKEGKILSSAAAFVEGGLQDACGDACSICLEDFCDSDPSTVTNCKHEFHLQCILEWCQ 997
            ETK E   L+SAAAFVEGG+QDAC D+CSICLE+F DSDPSTVT+CKHEFHLQCILEWCQ
Sbjct: 11   ETKSEAH-LTSAAAFVEGGIQDACDDSCSICLEEFIDSDPSTVTSCKHEFHLQCILEWCQ 69

Query: 996  RSSQCPMCWQALSLKDPISQELLEGVERERNLRASRSRN-TAFYHPALGDFELQHL 832
            RSSQCPMCWQ++SLKDP  QELLE VERERN+R + +RN T F+HP LGDFELQHL
Sbjct: 70   RSSQCPMCWQSISLKDPTGQELLEAVERERNIRFNPTRNATIFHHPTLGDFELQHL 125



 Score =  178 bits (452), Expect(2) = 6e-94
 Identities = 114/267 (42%), Positives = 147/267 (55%), Gaps = 16/267 (5%)
 Frame = -1

Query: 770 LPLGPNDAELEERIFQHLAAAAAMGRAQHIARRDGQRGRSAARGRPQYVVFSTNSSSVPA 591
           LP+G NDA+LEERI QH AAAAAMGRA+HIARR+GQR RS+A+GRPQY+VFS + +S P 
Sbjct: 125 LPVGANDADLEERILQHFAAAAAMGRARHIARREGQRNRSSAQGRPQYMVFSAHPNSPPM 184

Query: 590 XXXXXXXXXXXXXXXXXXXXXXXXXGNTLEESIPXXXXXXXXXXXXXXXXQPGPSSAATA 411
                                         E  P                  G ++ AT 
Sbjct: 185 APASSSPPQRGDGEQTP----------ATGEEPPQLTLVPPVQTDQVSASGSGSAALATD 234

Query: 410 RPVLXXXXXXXXXST----QDRAGPSDFQSFSETLKSRFNAVSLRYKESIANSTRGWKER 243
              L          +    QDRAGPS+ QSFSE+LKS+ NAVS RYKESI+ STRGWKER
Sbjct: 235 NLGLSYNSRRSPNQSSPSSQDRAGPSELQSFSESLKSKLNAVSTRYKESISKSTRGWKER 294

Query: 242 FFSRNTSAADLHSDTRRDVNTEVATVSRMMERLGSRNGIQATSPSL--------AQSTED 87
           +FSR +  +DL S+ +R+VN  +ATVSRMMERL +R+  +++S +          Q + D
Sbjct: 295 WFSRTSPMSDLGSEVKREVNAGIATVSRMMERLETRDNNRSSSDTAPSNLADGPIQGSSD 354

Query: 86  HTYSSVERVS----DNSANPATNGSSA 18
              +  ER S    +NS    T GSS+
Sbjct: 355 QHLTDTERDSPLRDNNSKTSCTAGSSS 381


>ref|XP_003540274.1| PREDICTED: E3 ubiquitin-protein ligase RHF2A-like [Glycine max]
          Length = 401

 Score =  196 bits (498), Expect(2) = 1e-68
 Identities = 92/117 (78%), Positives = 99/117 (84%), Gaps = 1/117 (0%)
 Frame = -3

Query: 1179 EETKKEGKILSSAAAFVEGGLQDACGDACSICLEDFCDSDPSTVTNCKHEFHLQCILEWC 1000
            +   K    L+SAAAFVEGG+QDAC DACSICLE FCDSDPSTVT+CKHEFHLQCILEWC
Sbjct: 16   DNNNKSEAHLTSAAAFVEGGIQDACDDACSICLEAFCDSDPSTVTSCKHEFHLQCILEWC 75

Query: 999  QRSSQCPMCWQALSLKDPISQELLEGVERERNLRASRSRN-TAFYHPALGDFELQHL 832
            QRSSQCPMCWQ +SLKDP SQELLE VERERN R + SRN T F+HP LGDFELQHL
Sbjct: 76   QRSSQCPMCWQPISLKDPTSQELLEAVERERNFRFNPSRNATIFHHPTLGDFELQHL 132



 Score = 91.7 bits (226), Expect(2) = 1e-68
 Identities = 43/59 (72%), Positives = 53/59 (89%)
 Frame = -1

Query: 770 LPLGPNDAELEERIFQHLAAAAAMGRAQHIARRDGQRGRSAARGRPQYVVFSTNSSSVP 594
           LP+G +DA+LEERI QHLAAAAAMGRA+HIARR+GQR RS+A+GRP ++VFSTN +S P
Sbjct: 132 LPVGASDADLEERIIQHLAAAAAMGRARHIARREGQRNRSSAQGRPHFLVFSTNPNSPP 190



 Score =  120 bits (301), Expect = 8e-25
 Identities = 62/114 (54%), Positives = 81/114 (71%)
 Frame = -1

Query: 368 TQDRAGPSDFQSFSETLKSRFNAVSLRYKESIANSTRGWKERFFSRNTSAADLHSDTRRD 189
           +QDRAGPS+ QSFSE+LKS+ NAVS RYKESI+ STRGWKER+FSRN + +DL S+ RR+
Sbjct: 271 SQDRAGPSELQSFSESLKSKLNAVSTRYKESISKSTRGWKERWFSRNNTMSDLGSEVRRE 330

Query: 188 VNTEVATVSRMMERLGSRNGIQATSPSLAQSTEDHTYSSVERVSDNSANPATNG 27
           VN  +ATVSRMMERL + +  ++ S S   + ED    SV+  +  +  P   G
Sbjct: 331 VNAGIATVSRMMERLETSDNNRSNSNSAPSNIED---GSVQEYNGQTLTPGNEG 381