BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dioscorea21_contig00010937
         (1388 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAK02913.1| predicted protein [Hordeum vulgare subsp. vulgare]    537   e-150
gb|ABI95365.1| flavonoid 3',5'-hydroxylase [Dendrobium hybrid cu...   525   e-146
gb|AAP31058.1| flavonoid 3',5'-hydroxylase [Gossypium hirsutum]       520   e-145
dbj|BAJ16328.1| flavonoid 3',5'-hydroxylase [Antirrhinum kelloggii]   520   e-145
ref|NP_001234840.1| putative flavonoid 3'5' hydroxylase [Solanum...   518   e-144

>dbj|BAK02913.1| predicted protein [Hordeum vulgare subsp. vulgare]
          Length = 519

 Score =  537 bits (1384), Expect = e-150
 Identities = 262/449 (58%), Positives = 345/449 (76%), Gaps = 10/449 (2%)
 Frame = +3

Query: 3    PIMYLKLGTCPVIVASTPSAARAFLTTLDSQFANRPTDIISAKDITYNCNNMVFSNLTPR 182
            P+MYL++GTC V+VAS+PSAAR FL  LD++FANRP  + SA DITY   NMVF++  P+
Sbjct: 71   PVMYLRMGTCGVVVASSPSAARTFLKALDARFANRPA-VASAADITYGRQNMVFADYGPK 129

Query: 183  WKLLRKLANLHMLGTKALSSWSPIRRSELHLMLQSIHSSCQL-GXXXXXXXXXXCATGNV 359
            WKL+RKLA++H+LG +A++ W+ +RR E   +L+ +  + ++ G          CA  N+
Sbjct: 130  WKLMRKLASVHLLGPRAVADWAGVRRDEAGRVLRGMKEAAEVTGRAVVVPEVLVCALANI 189

Query: 360  ISRVMLSRRVFDVRGEELGQFKSVLEDMLTGVGMFNIGDFVPSIAWMDLQGVQARMKGVN 539
            + ++ +SRRVFD +G+E   +K ++  +LTG G+FNI DFVP+++WMDLQGVQAR++ V+
Sbjct: 190  VGQITVSRRVFDAQGDESSSYKEMIVSLLTGTGLFNISDFVPALSWMDLQGVQARLRRVH 249

Query: 540  KRFDGMITRMLXXXXXXXXXXXDNP--DFIDLVLA--NRVGD----NGETLTDVNIKGLI 695
            ++FDG+IT++L            +   DF+D + A  N  GD    +GET+T+VNIKGLI
Sbjct: 250  RQFDGLITKLLAEHAATAEERARDGRLDFVDRLRASSNDGGDGEEGDGETITEVNIKGLI 309

Query: 696  WDMFTAGTDTSSIVVEWALAEMLKNPTILKRAHLEMDAVIGRDRLIDESDIPNLPYLRAI 875
            +DMFTAGTDTSSI+VEWA+AEM+ NP+I+ RA  EMD V+GR R ++ESDI NLPYL+AI
Sbjct: 310  FDMFTAGTDTSSIIVEWAMAEMINNPSIMARAQEEMDRVVGRGRRLEESDIANLPYLQAI 369

Query: 876  CKEALRKHPSTPLSLPHYSFQACEVEGYYIPANTRLLINIWGIGRDPNVWERPLEFDPER 1055
            CKEA+R HPSTPLSLPH+SF+ CEV+G+++PANTRLLINIW IGRDP  WE PLEF PER
Sbjct: 370  CKEAMRLHPSTPLSLPHFSFEECEVDGHHVPANTRLLINIWAIGRDPAAWEDPLEFRPER 429

Query: 1056 FISGKGAKIEPNGSDFELIPFGSGRRICAGKQAGMMFLQYFLGALVHAFDWVVPAGEE-I 1232
            F+SG  AKI+P G++FELIPFG+GRRICAGK AGM+F+QYFLG LVHAF+W +P GEE +
Sbjct: 430  FMSGPAAKIDPMGNNFELIPFGAGRRICAGKLAGMVFVQYFLGTLVHAFEWRLPDGEEKV 489

Query: 1233 DMSETPGLALPKAVPLKAFATPRLAPTVY 1319
            D +ET GLALPKAVPLKA  TPRL P  Y
Sbjct: 490  DTAETFGLALPKAVPLKALVTPRLVPEAY 518


>gb|ABI95365.1| flavonoid 3',5'-hydroxylase [Dendrobium hybrid cultivar]
          Length = 504

 Score =  525 bits (1352), Expect = e-146
 Identities = 254/441 (57%), Positives = 328/441 (74%), Gaps = 2/441 (0%)
 Frame = +3

Query: 3    PIMYLKLGTCPVIVASTPSAARAFLTTLDSQFANRPTDIISAKDITYNCNNMVFSNLTPR 182
            PIM+LK+G   V+VAS+ +AAR+FL T DS F++RP+ +IS K+I+YN  NMVF++  P+
Sbjct: 62   PIMFLKMGIRRVVVASSATAARSFLKTFDSHFSDRPSGVIS-KEISYNGQNMVFADYGPK 120

Query: 183  WKLLRKLANLHMLGTKALSSWSPIRRSELHLMLQSIHSSCQLGXXXXXXXXXXCATGNVI 362
            WKLLRK+++LH+LG+KA+S W+ +RR E   M+Q +                 CA  NVI
Sbjct: 121  WKLLRKVSSLHLLGSKAMSRWAGVRRDEAFSMIQFLKKHSDTEKPVLLPNLLVCAMANVI 180

Query: 363  SRVMLSRRVFDVRGEELGQFKSVLEDMLTGVGMFNIGDFVPSIAWMDLQGVQARMKGVNK 542
             R+ +S+RVFD  GEE  +FK +++++L G G  NIGD VPSI W+D  GV+ RM G+N+
Sbjct: 181  GRIAMSKRVFDEEGEEAKEFKEMIKELLVGQGASNIGDLVPSIGWLDPMGVKKRMLGLNR 240

Query: 543  RFDGMITRMLXXXXXXXXXXXDNPDFIDLVLANRV-GDNGETLTDVNIKGLIWDMFTAGT 719
            RFD M++++L            NPD +DLV+ + + G++GE L + NIKG I D+F AGT
Sbjct: 241  RFDRMVSKLLVEHAETAGERQGNPDLLDLVVGSELTGEDGEGLCEDNIKGFISDLFVAGT 300

Query: 720  DTSSIVVEWALAEMLKNPTILKRAHLEMDAVIGRDRLIDESDIPNLPYLRAICKEALRKH 899
            DTS+IV+EWA+AEMLKNP+IL+RA  E D V+GR RL+DESDIP LPYL+AICKEALRKH
Sbjct: 301  DTSAIVIEWAMAEMLKNPSILQRAQQETDRVVGRHRLLDESDIPKLPYLQAICKEALRKH 360

Query: 900  PSTPLSLPHYSFQACEVEGYYIPANTRLLINIWGIGRDPNVWERPLEFDPERFISGKGAK 1079
            P TPLS+PHY+ + CEVEGY+IP  T LL+NIW IGRDP+VWE PL FDPERF+ GK A+
Sbjct: 361  PPTPLSIPHYASEPCEVEGYHIPGKTWLLVNIWAIGRDPDVWENPLLFDPERFLQGKMAR 420

Query: 1080 IEPNGSDFELIPFGSGRRICAGKQAGMMFLQYFLGALVHAFDWVVPAG-EEIDMSETPGL 1256
            I+P G+DFELIPFG+GRRICAGK AGM+ +QY+LG LVHAFDW +P G  E+DM E PGL
Sbjct: 421  IDPMGNDFELIPFGAGRRICAGKLAGMLMVQYYLGTLVHAFDWSLPEGVGELDMEEGPGL 480

Query: 1257 ALPKAVPLKAFATPRLAPTVY 1319
             LPKAVPL   A PRLAP  Y
Sbjct: 481  VLPKAVPLSVMARPRLAPAAY 501


>gb|AAP31058.1| flavonoid 3',5'-hydroxylase [Gossypium hirsutum]
          Length = 510

 Score =  520 bits (1340), Expect = e-145
 Identities = 250/442 (56%), Positives = 325/442 (73%), Gaps = 1/442 (0%)
 Frame = +3

Query: 3    PIMYLKLGTCPVIVASTPSAARAFLTTLDSQFANRPTDIISAKDITYNCNNMVFSNLTPR 182
            P+MYLK+GTC ++VASTP AARAFL TLD  F+NRP++   A  I YN  +MVF+   PR
Sbjct: 70   PVMYLKMGTCNMVVASTPDAARAFLKTLDLNFSNRPSNA-GATHIAYNSQDMVFAEYGPR 128

Query: 183  WKLLRKLANLHMLGTKALSSWSPIRRSELHLMLQSIHSSCQLGXXXXXXXXXXCATGNVI 362
            WKLLRKL+NLHMLG KAL  WS +R  EL  ML+++  S + G           A  N+I
Sbjct: 129  WKLLRKLSNLHMLGGKALEDWSQVRAVELGHMLRAMCESSRKGEPVVVPEMLTYAMANMI 188

Query: 363  SRVMLSRRVFDVRGEELGQFKSVLEDMLTGVGMFNIGDFVPSIAWMDLQGVQARMKGVNK 542
             +V+LSRRVF  +G E  +FK ++ +++T  G+FNIGDF+PSIAWMDLQG++  MK ++ 
Sbjct: 189  GQVILSRRVFVTKGSESNEFKDMVVELMTSAGLFNIGDFIPSIAWMDLQGIEGEMKKLHN 248

Query: 543  RFDGMITRMLXXXXXXXXXXXDNPDFIDLVLANRVGDNGETLTDVNIKGLIWDMFTAGTD 722
            R+D ++T+M+             PDF+D+++ NR    GE L+  N+K L+ ++FTAGTD
Sbjct: 249  RWDVLLTKMMKEHEETAYERKGKPDFLDIIMDNRENSAGERLSLTNVKALLLNLFTAGTD 308

Query: 723  TSSIVVEWALAEMLKNPTILKRAHLEMDAVIGRDRLIDESDIPNLPYLRAICKEALRKHP 902
            TSS ++EWALAE+LKNP IL +AH EMD VIGR+R ++ESDIP LPYL+AICKE  RKHP
Sbjct: 309  TSSSIIEWALAEILKNPKILNKAHEEMDKVIGRNRRLEESDIPKLPYLQAICKETFRKHP 368

Query: 903  STPLSLPHYSFQACEVEGYYIPANTRLLINIWGIGRDPNVWERPLEFDPERFISGKGAKI 1082
            STPL+LP  S QACE+ GYYIP NTRL +NIW IGRDP+VW  PL+F PERF+SG+ AKI
Sbjct: 369  STPLNLPRVSTQACEINGYYIPKNTRLSVNIWAIGRDPDVWGNPLDFTPERFLSGRFAKI 428

Query: 1083 EPNGSDFELIPFGSGRRICAGKQAGMMFLQYFLGALVHAFDWVVPAGE-EIDMSETPGLA 1259
            +P G+DFELIPFG+GRRICAG + G++ ++Y LG L+H+FDW++P G  E++M E  GLA
Sbjct: 429  DPRGNDFELIPFGAGRRICAGTRMGIVLVEYILGTLLHSFDWMLPPGTGELNMDEAFGLA 488

Query: 1260 LPKAVPLKAFATPRLAPTVYMS 1325
            L KAVPL A   PRLAPT Y+S
Sbjct: 489  LQKAVPLSAMVRPRLAPTAYVS 510


>dbj|BAJ16328.1| flavonoid 3',5'-hydroxylase [Antirrhinum kelloggii]
          Length = 510

 Score =  520 bits (1338), Expect = e-145
 Identities = 252/440 (57%), Positives = 325/440 (73%), Gaps = 1/440 (0%)
 Frame = +3

Query: 3    PIMYLKLGTCPVIVASTPSAARAFLTTLDSQFANRPTDIISAKDITYNCNNMVFSNLTPR 182
            P++YLK+G   + VASTP +A+AFL TLD+ F+NRP +   A  + YN  +MVF+   PR
Sbjct: 70   PVIYLKVGAHGMAVASTPESAKAFLKTLDTNFSNRPPNA-GATHLAYNSQDMVFAAYGPR 128

Query: 183  WKLLRKLANLHMLGTKALSSWSPIRRSELHLMLQSIHSSCQLGXXXXXXXXXXCATGNVI 362
            W+LLRKL+NLHMLGTKAL  W+ +R SE+  ML+ +H +   G           A  N+I
Sbjct: 129  WRLLRKLSNLHMLGTKALDDWANVRVSEVGYMLEDMHGASGRGEAVGVPGMLVYAMANMI 188

Query: 363  SRVMLSRRVFDVRGEELGQFKSVLEDMLTGVGMFNIGDFVPSIAWMDLQGVQARMKGVNK 542
             +V+LSRRVF  RGEEL +FK ++ +++T  G FNIGDF+PS AWMDLQG++  MKG++K
Sbjct: 189  GQVILSRRVFVTRGEELNEFKDMVVELMTSAGYFNIGDFIPSFAWMDLQGIEKGMKGLHK 248

Query: 543  RFDGMITRMLXXXXXXXXXXXDNPDFIDLVLANRVGDNGETLTDVNIKGLIWDMFTAGTD 722
            +FD +I+RML           + PDF+D++LANR    GE LT  NIK L+ ++FTAGTD
Sbjct: 249  KFDDLISRMLEEHLASAHIRKEKPDFLDVILANRDTLEGERLTTSNIKALLLNLFTAGTD 308

Query: 723  TSSIVVEWALAEMLKNPTILKRAHLEMDAVIGRDRLIDESDIPNLPYLRAICKEALRKHP 902
            TSS  +EWALAEM+KNP ILK+AH EMD V+GR+R + ESDIP LPYL+AICKE+ RKHP
Sbjct: 309  TSSSTIEWALAEMIKNPAILKKAHDEMDQVVGRNRRLMESDIPKLPYLQAICKESFRKHP 368

Query: 903  STPLSLPHYSFQACEVEGYYIPANTRLLINIWGIGRDPNVWERPLEFDPERFISGKGAKI 1082
            STPL+LP  S QAC V GYYIP NTRL +NIW IGRDPNVWE PLEF+P+RF+SGK AK+
Sbjct: 369  STPLNLPRISSQACTVNGYYIPKNTRLNVNIWAIGRDPNVWENPLEFNPDRFMSGKNAKL 428

Query: 1083 EPNGSDFELIPFGSGRRICAGKQAGMMFLQYFLGALVHAFDWVVPAG-EEIDMSETPGLA 1259
            +P G+DFELIPFG+GRRICAG + G++ ++Y LG+LVH+FDW +P G +E+++ E  GLA
Sbjct: 429  DPRGNDFELIPFGAGRRICAGARMGIVLVEYILGSLVHSFDWKLPEGVKEMNLDEAFGLA 488

Query: 1260 LPKAVPLKAFATPRLAPTVY 1319
            L KAVPL A  TPRL    Y
Sbjct: 489  LQKAVPLAAMVTPRLPSNCY 508


>ref|NP_001234840.1| putative flavonoid 3'5' hydroxylase [Solanum lycopersicum]
            gi|194021495|gb|ACF32346.1| putative flavonoid 3'5'
            hydroxylase [Solanum lycopersicum]
            gi|289064208|gb|ADC80513.1| flavonoid 3`,5`-hydroxylase
            [Solanum lycopersicum]
          Length = 511

 Score =  518 bits (1335), Expect = e-144
 Identities = 252/440 (57%), Positives = 327/440 (74%), Gaps = 1/440 (0%)
 Frame = +3

Query: 3    PIMYLKLGTCPVIVASTPSAARAFLTTLDSQFANRPTDIISAKDITYNCNNMVFSNLTPR 182
            PIMYLK+GTC ++VASTP+AA+AFL TLD  F+NRP +   A  + YN  +MVF+   PR
Sbjct: 71   PIMYLKVGTCGMVVASTPNAAKAFLKTLDINFSNRPPNA-GATHLAYNAQDMVFAPYGPR 129

Query: 183  WKLLRKLANLHMLGTKALSSWSPIRRSELHLMLQSIHSSCQLGXXXXXXXXXXCATGNVI 362
            WKLLRKL+NLHMLG KAL +W+ +R +EL  ML+S+  + Q G           A  N+I
Sbjct: 130  WKLLRKLSNLHMLGGKALENWANVRANELGHMLKSMFDASQDGECVVIADVLTFAMANMI 189

Query: 363  SRVMLSRRVFDVRGEELGQFKSVLEDMLTGVGMFNIGDFVPSIAWMDLQGVQARMKGVNK 542
             +VMLS+RVF  +G E+ +FK+++ +++T  G FNIGDF+P +AWMD+QG++  MK ++K
Sbjct: 190  GQVMLSKRVFVEKGVEVNEFKNMVVELMTVAGYFNIGDFIPKLAWMDIQGIEKGMKNLHK 249

Query: 543  RFDGMITRMLXXXXXXXXXXXDNPDFIDLVLANRVGDNGETLTDVNIKGLIWDMFTAGTD 722
            +FD ++T+M            +NPDF+D+V+ANR    GE L+  NIK L+ ++FTAGTD
Sbjct: 250  KFDDLLTKMFDEHEATSNERKENPDFLDVVMANRDNSEGERLSTTNIKALLLNLFTAGTD 309

Query: 723  TSSIVVEWALAEMLKNPTILKRAHLEMDAVIGRDRLIDESDIPNLPYLRAICKEALRKHP 902
            TSS V+EWALAEM+KNP I K+A  EMD VIG++R + ESDIPNLPYLRAICKE  RKHP
Sbjct: 310  TSSSVIEWALAEMMKNPKIFKKAQQEMDQVIGKNRRLIESDIPNLPYLRAICKETFRKHP 369

Query: 903  STPLSLPHYSFQACEVEGYYIPANTRLLINIWGIGRDPNVWERPLEFDPERFISGKGAKI 1082
            STPL+LP  S + C V+GYYIP NTRL +NIW IGRDP+VWE PLEF PERF+SGK AKI
Sbjct: 370  STPLNLPRVSSEPCTVDGYYIPKNTRLSVNIWAIGRDPDVWENPLEFTPERFLSGKNAKI 429

Query: 1083 EPNGSDFELIPFGSGRRICAGKQAGMMFLQYFLGALVHAFDWVVPAGE-EIDMSETPGLA 1259
            EP G+DFELIPFG+GRRICAG + G++ ++Y LG LVH+FDW +P    +I+M E+ GLA
Sbjct: 430  EPRGNDFELIPFGAGRRICAGTRMGIVVVEYILGTLVHSFDWKLPNNVIDINMEESFGLA 489

Query: 1260 LPKAVPLKAFATPRLAPTVY 1319
            L KAVPL+A  TPRL+  VY
Sbjct: 490  LQKAVPLEAMVTPRLSLDVY 509