BLASTX nr result
ID: Dioscorea21_contig00009746
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dioscorea21_contig00009746
(1841 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002280960.2| PREDICTED: DNA topoisomerase 1-like [Vitis v... 597 e-168
ref|XP_003560815.1| PREDICTED: DNA topoisomerase 1-like [Brachyp... 597 e-168
gb|EAZ36631.1| hypothetical protein OsJ_20977 [Oryza sativa Japo... 595 e-167
ref|XP_003545557.1| PREDICTED: DNA topoisomerase 1-like [Glycine... 579 e-163
ref|XP_002436546.1| hypothetical protein SORBIDRAFT_10g004493 [S... 579 e-163
>ref|XP_002280960.2| PREDICTED: DNA topoisomerase 1-like [Vitis vinifera]
Length = 1185
Score = 597 bits (1539), Expect = e-168
Identities = 299/450 (66%), Positives = 349/450 (77%), Gaps = 1/450 (0%)
Frame = +3
Query: 477 QEAHEAIRPTNIRRLPSSLNGILDDDALKLYTLIWARTMACQMESLLIDTVQVDIGSMGG 656
QEAHEAIRPT+I+RLPS L G+LD+D+LKLYTLIW+RTMACQME ID +QVDIG+
Sbjct: 733 QEAHEAIRPTDIQRLPSMLAGVLDEDSLKLYTLIWSRTMACQMEPATIDQIQVDIGNANE 792
Query: 657 DLVLRSTGSKVGFPGYQAVYKDKEAGVNGNDDKVEDVTQACFDVLRKLKVEDQLSSGEVE 836
+V RST S V F GYQAVYKD EA D+ + F L LK D L +E
Sbjct: 793 SVVFRSTCSGVEFFGYQAVYKDVEAKAIRVDENEGNERGEVFKALSSLKAGDPLYLSLLE 852
Query: 837 LRQHHTKPPPRYSEGSLVKKLEELGIGRPSTYASIMKVLQDRNYMTVKNRVLFPEFRGRM 1016
L QHHT PP RYSEG+LVKKLEELGIGRPSTYA MKVLQDRNY+TVKNRVL+PEFRGRM
Sbjct: 853 LEQHHTLPPSRYSEGALVKKLEELGIGRPSTYAITMKVLQDRNYVTVKNRVLYPEFRGRM 912
Query: 1017 VSIFLSHHFAEVADYSFTADMENELDNVSGGVTEWKGLLKDYWSRFSKYCDLATSTDIRQ 1196
VS FLSHHF+EV DYSFTADME ELDNVS G+TEWKGLLKDYW+RFS YC+ ++ I Q
Sbjct: 913 VSAFLSHHFSEVTDYSFTADMETELDNVSAGLTEWKGLLKDYWTRFSMYCNRVSNVHIHQ 972
Query: 1197 VEKMLEEKFGHILFASLPDQSRVCPSCSRGTLKFKVSRFGAGYFIGCDQHPSCKHISSTV 1376
VEKMLE+KFG LF+ LPD+SR CPSC GTL FKVSRFG+GYFIGCDQHP CK+I+ +
Sbjct: 973 VEKMLEKKFGDFLFSFLPDKSRTCPSCMEGTLIFKVSRFGSGYFIGCDQHPKCKYIAKML 1032
Query: 1377 FSDEDESHASDNPEKSF-PPKLLGYCPDSNNKVLLKNGPYGFYVQLGDDTHGYTPKRASV 1553
D+DE AS +K+F PK LG P S+ K+LLKNGPYGFY+QLG+D GY PKRASV
Sbjct: 1033 DGDDDEEVASQ--DKTFEEPKALGLSPGSSEKILLKNGPYGFYLQLGEDRKGYLPKRASV 1090
Query: 1554 TEVKDVDSITLEFALDLLQYPLTLGKHPEDKHPVMLAHSKFGFSVKHRRTIAPVPKSVNP 1733
+ +KDV SITLE AL+LL+YP+TLG HP D HPV+L +K GFS++HRRTIAPVPK++ P
Sbjct: 1091 SHIKDVGSITLEDALELLRYPVTLGNHPNDDHPVVLKLAKNGFSIRHRRTIAPVPKNIKP 1150
Query: 1734 KSITLQTGLKLLSGKNVRRSGRPKRKPNTE 1823
ITL+ LKLL GK+V++SGRPK K E
Sbjct: 1151 NDITLEKALKLLLGKDVKQSGRPKNKKKQE 1180
Score = 131 bits (330), Expect = 5e-28
Identities = 66/113 (58%), Positives = 80/113 (70%)
Frame = +1
Query: 1 RKLNQLXXXXXXXXXXXXXXXXXXQFKVLAVKMGKVTKNPPMPYITSTLQQDSASKLNFS 180
+KLNQ +FKV+ K K+ KNPP PYITSTLQQD+A+KL+FS
Sbjct: 602 KKLNQFSISSHTEAKAIEQEINSLEFKVIGSKRNKMRKNPPTPYITSTLQQDAANKLHFS 661
Query: 181 TTYTMKLAQKLYEGIKLSDEEATGLITYMRTDGLNISNEAANDIHSFVKERYG 339
YTMKLAQ+LYEG++LSD +A GLITYMRTDGL++S+EAA DI S V ERYG
Sbjct: 662 AMYTMKLAQRLYEGVQLSDGKAAGLITYMRTDGLHVSDEAAKDIRSLVAERYG 714
>ref|XP_003560815.1| PREDICTED: DNA topoisomerase 1-like [Brachypodium distachyon]
Length = 877
Score = 597 bits (1539), Expect = e-168
Identities = 287/447 (64%), Positives = 357/447 (79%)
Frame = +3
Query: 477 QEAHEAIRPTNIRRLPSSLNGILDDDALKLYTLIWARTMACQMESLLIDTVQVDIGSMGG 656
QEAHEAIRPT+IRRLPSSL GILD+D+LKLY LIW RTMACQME+ + +QVDIG+ GG
Sbjct: 422 QEAHEAIRPTSIRRLPSSLVGILDEDSLKLYALIWRRTMACQMEASRTELIQVDIGNPGG 481
Query: 657 DLVLRSTGSKVGFPGYQAVYKDKEAGVNGNDDKVEDVTQACFDVLRKLKVEDQLSSGEVE 836
D+ S+ SK+ F GYQAVY D EA + + + E + F+ L KL+++D +S V
Sbjct: 482 DMTFHSSASKLDFKGYQAVYDDTEASGSSENPEGEALHHDNFEALSKLEMKDLVSPVNVH 541
Query: 837 LRQHHTKPPPRYSEGSLVKKLEELGIGRPSTYASIMKVLQDRNYMTVKNRVLFPEFRGRM 1016
L QH TKPP RYSEG+L+KKLEELGIGRPSTYASIM+VLQDR Y+T+K+RVL PEFRGRM
Sbjct: 542 LGQHFTKPPSRYSEGALIKKLEELGIGRPSTYASIMRVLQDRKYVTIKSRVLHPEFRGRM 601
Query: 1017 VSIFLSHHFAEVADYSFTADMENELDNVSGGVTEWKGLLKDYWSRFSKYCDLATSTDIRQ 1196
VS FLSHHF+E+ADYSFTA+ME ELDNVS G TEWKGLLKDYW RFSKYC A+ D+R+
Sbjct: 602 VSAFLSHHFSEIADYSFTANMETELDNVSAGSTEWKGLLKDYWERFSKYCGDASQWDVRK 661
Query: 1197 VEKMLEEKFGHILFASLPDQSRVCPSCSRGTLKFKVSRFGAGYFIGCDQHPSCKHISSTV 1376
VE+MLEEKF ILF L D SR+CPSCS GTL+FKVSRFG GYFIGCD+HP CK+I+ T+
Sbjct: 662 VERMLEEKFRPILFPDLDDASRICPSCSEGTLRFKVSRFGEGYFIGCDRHPKCKYIARTL 721
Query: 1377 FSDEDESHASDNPEKSFPPKLLGYCPDSNNKVLLKNGPYGFYVQLGDDTHGYTPKRASVT 1556
++DE+ A + + +F P+LLG P+ N KV LK GPYG+Y+Q+G+D G + KRAS++
Sbjct: 722 SDEDDENEACEETKWNFEPRLLGVMPNLNEKVFLKQGPYGYYIQVGEDRKGISQKRASLS 781
Query: 1557 EVKDVDSITLEFALDLLQYPLTLGKHPEDKHPVMLAHSKFGFSVKHRRTIAPVPKSVNPK 1736
EVK+VDSITLE A++LLQYP LGKHP+D++PV++ HSK GFSV+HRRT+AP+PK+ + K
Sbjct: 782 EVKNVDSITLEDAIELLQYPKILGKHPDDEYPVLMTHSKAGFSVRHRRTVAPLPKNTDLK 841
Query: 1737 SITLQTGLKLLSGKNVRRSGRPKRKPN 1817
ITL+ LKLL+GKNV++ GRP K N
Sbjct: 842 KITLECALKLLAGKNVKKFGRPLGKQN 868
Score = 131 bits (329), Expect = 7e-28
Identities = 67/113 (59%), Positives = 81/113 (71%)
Frame = +1
Query: 1 RKLNQLXXXXXXXXXXXXXXXXXXQFKVLAVKMGKVTKNPPMPYITSTLQQDSASKLNFS 180
+KL+QL QF+V VK K+ KNPPMPYITS+LQQD+A+KL+FS
Sbjct: 291 KKLDQLSISSQEEARAIEKRIYSSQFEVRGVKRSKIHKNPPMPYITSSLQQDAANKLHFS 350
Query: 181 TTYTMKLAQKLYEGIKLSDEEATGLITYMRTDGLNISNEAANDIHSFVKERYG 339
+TMK+AQKLYEGI LS EEATGLITY+RTDG +IS+ AA D+ S VKERYG
Sbjct: 351 AGHTMKVAQKLYEGISLSSEEATGLITYLRTDGFHISDGAAEDVRSLVKERYG 403
>gb|EAZ36631.1| hypothetical protein OsJ_20977 [Oryza sativa Japonica Group]
Length = 1180
Score = 595 bits (1535), Expect = e-167
Identities = 292/466 (62%), Positives = 358/466 (76%), Gaps = 17/466 (3%)
Frame = +3
Query: 477 QEAHEAIRPTNIRRLPSSLNGILDDDALKLYTLIWARTMACQMESLLIDTVQVDIGSMGG 656
QEAHEAIRPT+IRRLPSSL G LDDD+LKLYTLIW RTMACQME+ D +QVDIG+ G
Sbjct: 708 QEAHEAIRPTSIRRLPSSLVGALDDDSLKLYTLIWKRTMACQMEASRTDMIQVDIGNSEG 767
Query: 657 DLVLRSTGSKVGFPGYQAVYKDKEAGVNGNDDKVEDVTQACFDVLRKLKVEDQLSSGEVE 836
D++ S+ S++ F GYQAVY D EA + + +V+ V Q F+ L KL+V+D +S V
Sbjct: 768 DMIFHSSASRLDFKGYQAVYDDTEASPSSYNSEVDAVHQDNFEALSKLEVKDLVSPVNVH 827
Query: 837 LRQHHTKPPPRYSEGSLVKKLEELGIGRPSTYASIMKVLQDRNYMTVKNRVLFPEFRGRM 1016
L QH TKPP RYSE +L+KKLEELGIGRPSTYASIMKVLQDR Y+T+K+RVL PEFRGRM
Sbjct: 828 LSQHFTKPPSRYSESALIKKLEELGIGRPSTYASIMKVLQDRKYVTIKSRVLHPEFRGRM 887
Query: 1017 VSIFLSHHFAEVADYSFTADMENELDNVSGGVTEWKGLLKDYWSRFSKYCDLATSTDIR- 1193
VS FL HHF+EVAD SFTA+ME ELDNVS G TEWKGLLKD+W RF+KYC A+ D+R
Sbjct: 888 VSAFLMHHFSEVADLSFTANMETELDNVSAGSTEWKGLLKDFWERFNKYCGDASRLDVRK 947
Query: 1194 ----------------QVEKMLEEKFGHILFASLPDQSRVCPSCSRGTLKFKVSRFGAGY 1325
+VE+MLEEKFG ILF+ L + SR+CPSCS GTL+FKVSR+G GY
Sbjct: 948 VERKSLLISHKVQLPLKVERMLEEKFGSILFSDLDNDSRICPSCSEGTLRFKVSRYGEGY 1007
Query: 1326 FIGCDQHPSCKHISSTVFSDEDESHASDNPEKSFPPKLLGYCPDSNNKVLLKNGPYGFYV 1505
FIGCD+HP CK+I+ T+ D+DE+ ASD +++F P+LLG PDS+ KV LK GPYG YV
Sbjct: 1008 FIGCDRHPKCKYIARTLSDDDDETEASDETQRTFTPRLLGALPDSDEKVFLKQGPYGHYV 1067
Query: 1506 QLGDDTHGYTPKRASVTEVKDVDSITLEFALDLLQYPLTLGKHPEDKHPVMLAHSKFGFS 1685
Q+G+D G +PKRA ++EVKD+DSITL+ A++LLQYP LGKHP+D PV++ HSK GFS
Sbjct: 1068 QVGEDRKGVSPKRAPLSEVKDIDSITLKDAIELLQYPKILGKHPDDDLPVLITHSKAGFS 1127
Query: 1686 VKHRRTIAPVPKSVNPKSITLQTGLKLLSGKNVRRSGRPKRKPNTE 1823
++HRRT+A +PKS +PK ITL+ LKLL+GKNVR GRPK K E
Sbjct: 1128 IRHRRTLASLPKSADPKKITLERALKLLTGKNVRMFGRPKGKTKKE 1173
Score = 110 bits (275), Expect = 1e-21
Identities = 55/95 (57%), Positives = 67/95 (70%)
Frame = +1
Query: 1 RKLNQLXXXXXXXXXXXXXXXXXXQFKVLAVKMGKVTKNPPMPYITSTLQQDSASKLNFS 180
+KL+QL QF+V +K K+ KNPPMPYITS+LQQD+A+KL+FS
Sbjct: 524 KKLDQLSIRSQEEAHNIEKRIYSSQFEVTGIKRSKINKNPPMPYITSSLQQDAANKLHFS 583
Query: 181 TTYTMKLAQKLYEGIKLSDEEATGLITYMRTDGLN 285
YTMK+AQKLYEGI LS EEATGLITY+RTDG +
Sbjct: 584 AGYTMKVAQKLYEGINLSSEEATGLITYIRTDGFH 618
>ref|XP_003545557.1| PREDICTED: DNA topoisomerase 1-like [Glycine max]
Length = 1197
Score = 579 bits (1493), Expect = e-163
Identities = 285/447 (63%), Positives = 350/447 (78%), Gaps = 3/447 (0%)
Frame = +3
Query: 477 QEAHEAIRPTNIRRLPSSLNGILDDDALKLYTLIWARTMACQMESLLIDTVQVDIGSMGG 656
QEAHEAIRPT+IR+LPS L+ ILD+++LKLYTLIW+RT++CQME + + +Q+DIG++
Sbjct: 741 QEAHEAIRPTDIRKLPSMLSAILDEESLKLYTLIWSRTVSCQMEPAVFEQIQLDIGNLDE 800
Query: 657 DLVLRSTGSKVGFPGYQAVYKD---KEAGVNGNDDKVEDVTQACFDVLRKLKVEDQLSSG 827
++L+S+ S+V FPGY+AV+ D + G+D+ D T F VL LK D L
Sbjct: 801 SIILQSSISRVEFPGYRAVFTDIVSEAVQDKGSDESSHDQT---FGVLNSLKTGDSLHVV 857
Query: 828 EVELRQHHTKPPPRYSEGSLVKKLEELGIGRPSTYASIMKVLQDRNYMTVKNRVLFPEFR 1007
+ E QHHT+PPPRYSE SLVKKLEELGIGRPSTYAS +KVLQDRNY+TVK+RVL PEFR
Sbjct: 858 QTEPNQHHTQPPPRYSEASLVKKLEELGIGRPSTYASTIKVLQDRNYVTVKSRVLSPEFR 917
Query: 1008 GRMVSIFLSHHFAEVADYSFTADMENELDNVSGGVTEWKGLLKDYWSRFSKYCDLATSTD 1187
GRMVS FLS+HF+EV DYSFTADME ELDNVS G+TEWKGLL+DYW+RF YC+ ++
Sbjct: 918 GRMVSAFLSNHFSEVTDYSFTADMETELDNVSAGLTEWKGLLRDYWTRFKSYCERTSNVH 977
Query: 1188 IRQVEKMLEEKFGHILFASLPDQSRVCPSCSRGTLKFKVSRFGAGYFIGCDQHPSCKHIS 1367
I QVEKMLE+KFG LFASLPDQSRVCPSC GTL FKVSRFGAGYFIGCDQHP CK+I+
Sbjct: 978 IHQVEKMLEKKFGDYLFASLPDQSRVCPSCMEGTLIFKVSRFGAGYFIGCDQHPRCKYIA 1037
Query: 1368 STVFSDEDESHASDNPEKSFPPKLLGYCPDSNNKVLLKNGPYGFYVQLGDDTHGYTPKRA 1547
T++ E+E AS + PKLLG SN KVLLK+GPYG YVQLG+D GY P+RA
Sbjct: 1038 KTLYGTEEEEDASQPNTRIEEPKLLGVTSGSNEKVLLKSGPYGVYVQLGEDRKGYIPRRA 1097
Query: 1548 SVTEVKDVDSITLEFALDLLQYPLTLGKHPEDKHPVMLAHSKFGFSVKHRRTIAPVPKSV 1727
SV+ +KDVDSITL+ AL++LQYPLTLG HP+D PV+L ++ GF V+HRRTIA +PK+V
Sbjct: 1098 SVSHIKDVDSITLKDALEVLQYPLTLGNHPKDGQPVILRLARAGFCVRHRRTIASIPKNV 1157
Query: 1728 NPKSITLQTGLKLLSGKNVRRSGRPKR 1808
P +TL+ L+ LSG +VRRSGRPK+
Sbjct: 1158 KPSDVTLEKALEYLSGDDVRRSGRPKK 1184
Score = 117 bits (293), Expect = 1e-23
Identities = 56/88 (63%), Positives = 72/88 (81%)
Frame = +1
Query: 76 FKVLAVKMGKVTKNPPMPYITSTLQQDSASKLNFSTTYTMKLAQKLYEGIKLSDEEATGL 255
F V+++K K +NPP PYITSTLQQD+A+KL+F+ ++TMKLAQKLYEG++LSD A GL
Sbjct: 635 FHVVSLKKNKSRRNPPTPYITSTLQQDAANKLHFTASHTMKLAQKLYEGVELSDSVAVGL 694
Query: 256 ITYMRTDGLNISNEAANDIHSFVKERYG 339
ITY+RTDGL+IS+EA I S + ERYG
Sbjct: 695 ITYIRTDGLHISDEAVASIRSLIIERYG 722
>ref|XP_002436546.1| hypothetical protein SORBIDRAFT_10g004493 [Sorghum bicolor]
gi|241914769|gb|EER87913.1| hypothetical protein
SORBIDRAFT_10g004493 [Sorghum bicolor]
Length = 1094
Score = 579 bits (1493), Expect = e-163
Identities = 280/432 (64%), Positives = 342/432 (79%)
Frame = +3
Query: 477 QEAHEAIRPTNIRRLPSSLNGILDDDALKLYTLIWARTMACQMESLLIDTVQVDIGSMGG 656
QEAHEAIRPT+IRRLPSSL GILDDD+LKLYTLIW RTMACQME+ + +QVDIG+ G
Sbjct: 663 QEAHEAIRPTSIRRLPSSLVGILDDDSLKLYTLIWKRTMACQMEASRTEMIQVDIGTPEG 722
Query: 657 DLVLRSTGSKVGFPGYQAVYKDKEAGVNGNDDKVEDVTQACFDVLRKLKVEDQLSSGEVE 836
+++ S SK+ F GYQAVY+D EA ++ + + V + F+ L KL V+D + V
Sbjct: 723 EMIFHSVASKLDFKGYQAVYEDTEAIPCSDNSERDSVDEDNFEALSKLMVKDLVFPVNVH 782
Query: 837 LRQHHTKPPPRYSEGSLVKKLEELGIGRPSTYASIMKVLQDRNYMTVKNRVLFPEFRGRM 1016
L QH TKPP RYSEG+L+KKLEELGIGRPSTYASIMKVLQDR Y+T K+RVL PEFRGRM
Sbjct: 783 LGQHFTKPPSRYSEGALIKKLEELGIGRPSTYASIMKVLQDRKYVTAKSRVLHPEFRGRM 842
Query: 1017 VSIFLSHHFAEVADYSFTADMENELDNVSGGVTEWKGLLKDYWSRFSKYCDLATSTDIRQ 1196
VS FL HHF+EVADYSFTA+ME ELDNVS G TEWKGLLKDYW RF+KYC A+ D R+
Sbjct: 843 VSAFLLHHFSEVADYSFTANMETELDNVSAGSTEWKGLLKDYWERFNKYCADASKLDGRK 902
Query: 1197 VEKMLEEKFGHILFASLPDQSRVCPSCSRGTLKFKVSRFGAGYFIGCDQHPSCKHISSTV 1376
VE+MLEEKFG ILF + SR+CPSCS GTL+FKVSR+G GYFIGCD+HP CK+I+ ++
Sbjct: 903 VERMLEEKFGPILFPDVDKDSRICPSCSEGTLRFKVSRYGEGYFIGCDRHPKCKYIARSL 962
Query: 1377 FSDEDESHASDNPEKSFPPKLLGYCPDSNNKVLLKNGPYGFYVQLGDDTHGYTPKRASVT 1556
EDE+ ++ KSF P+LLG PDS+ KV LK GPYG+YVQ+G+D G PKRAS++
Sbjct: 963 SQQEDETEPTEESPKSFTPRLLGVMPDSDQKVFLKQGPYGYYVQIGEDKKGLFPKRASLS 1022
Query: 1557 EVKDVDSITLEFALDLLQYPLTLGKHPEDKHPVMLAHSKFGFSVKHRRTIAPVPKSVNPK 1736
EVKD+DS+TLE A++LLQYP LGKHPED HPV++ HSK G+++KHRR++A VPKS +PK
Sbjct: 1023 EVKDIDSVTLEDAIELLQYPKILGKHPEDDHPVLITHSKVGYNIKHRRSLALVPKSTDPK 1082
Query: 1737 SITLQTGLKLLS 1772
+TL+ LKLLS
Sbjct: 1083 KMTLERALKLLS 1094
Score = 131 bits (329), Expect = 7e-28
Identities = 66/113 (58%), Positives = 81/113 (71%)
Frame = +1
Query: 1 RKLNQLXXXXXXXXXXXXXXXXXXQFKVLAVKMGKVTKNPPMPYITSTLQQDSASKLNFS 180
+KL+QL +F+VL VK K+ KNPPMPYITS+LQQD+A+KL+F+
Sbjct: 532 KKLDQLSIRSQEEAQAIEKRIHSSKFEVLGVKRSKIHKNPPMPYITSSLQQDAANKLHFT 591
Query: 181 TTYTMKLAQKLYEGIKLSDEEATGLITYMRTDGLNISNEAANDIHSFVKERYG 339
YTMK+AQKLYEGI LS EE TGLITY+RTDG ++S+ AA DI S VKERYG
Sbjct: 592 AGYTMKIAQKLYEGINLSSEETTGLITYIRTDGFHVSDGAAEDIRSLVKERYG 644