BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dioscorea21_contig00009675
         (883 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002285105.1| PREDICTED: isoflavone 2'-hydroxylase-like [V...   289   5e-76
ref|XP_004143022.1| PREDICTED: cytochrome P450 81D1-like [Cucumi...   287   3e-75
ref|XP_002327185.1| cytochrome P450 [Populus trichocarpa] gi|222...   283   3e-74
gb|ADD84651.1| CYP81B36 [Scoparia dulcis]                             283   4e-74
dbj|BAF98467.1| cytochrome P450 [Coptis japonica var. dissecta]       281   2e-73

>ref|XP_002285105.1| PREDICTED: isoflavone 2'-hydroxylase-like [Vitis vinifera]
          Length = 499

 Score =  289 bits (740), Expect = 5e-76
 Identities = 139/295 (47%), Positives = 213/295 (72%), Gaps = 1/295 (0%)
 Frame = +1

Query: 1    VEEVFLLSGASTLEDFIPVVRWLRIGGAEKRMERVGKELDEFYQKIIEDRRRVGKWKEYV 180
            V E F L+GA+ + DF+P++ WL + G E+R+ ++ K  + F Q +IE+ RR G      
Sbjct: 205  VSETFQLAGATNMVDFLPILGWLGLKGTERRLIKLQKRRESFIQNLIEEHRRKGS----- 259

Query: 181  DGHDQEKKGNIIDVMLAMQEKEKDNYSDVAIKGVMTSMLXXXXXXXXXXMEWVMALLLNH 360
              + + ++  +I+V+L++QE E + Y+D  I+G+M SML          MEW ++LLLN+
Sbjct: 260  --NCEGRQKTMIEVLLSLQETEPEYYTDEIIRGLMLSMLTGGTDTSAGTMEWALSLLLNN 317

Query: 361  PNALKKAKAEIKEQVGHSHLIKDSDISKLHYLDNVIKETLRLFPAGPLLVAHESSQDCTV 540
            P  LKKA  EI +++GH  LI++ D+++L YL ++IKETLR++PAGPLLV HESS++C+V
Sbjct: 318  PKVLKKAHQEIDDRLGHDRLIEELDLAQLPYLRSIIKETLRMYPAGPLLVPHESSKECSV 377

Query: 541  LGSHIPKGTMLLVNAYAMQRDNQLWDNPLEFKPERFDSDDHVLHGD-EGYKYIPFGTGRR 717
             G  IP+GTMLLVN +A+Q D+++W +P EF+PERF+     + GD +G+K++PFG+GRR
Sbjct: 378  GGFRIPQGTMLLVNLWAIQSDHKIWGDPTEFRPERFEG----VEGDRDGFKFVPFGSGRR 433

Query: 718  RCPGESLAWKVMLLTLGALIQCFEWERVGKELVDLSEGVGLSLPMAKPLQAMYKP 882
             CPGE+LA +++ L LG+LIQCF+WERV +++VD++EG GL+LP A+PL A  +P
Sbjct: 434  GCPGEALAIRIVGLALGSLIQCFDWERVDEQMVDMTEGGGLTLPKAQPLLAKCRP 488


>ref|XP_004143022.1| PREDICTED: cytochrome P450 81D1-like [Cucumis sativus]
            gi|449482809|ref|XP_004156410.1| PREDICTED: cytochrome
            P450 81D1-like [Cucumis sativus]
          Length = 522

 Score =  287 bits (734), Expect = 3e-75
 Identities = 146/296 (49%), Positives = 204/296 (68%), Gaps = 2/296 (0%)
 Frame = +1

Query: 1    VEEVFLLSGASTLEDFIP-VVRWLRI-GGAEKRMERVGKELDEFYQKIIEDRRRVGKWKE 174
            V+E F +SGA+ + DF+P  ++W+ +  G EKRMER+    D F Q +IE+RRR+ + K 
Sbjct: 223  VKEGFKVSGATNMGDFLPNFLKWVGLWSGIEKRMERLQGLRDGFMQNLIEERRRMRRDK- 281

Query: 175  YVDGHDQEKKGNIIDVMLAMQEKEKDNYSDVAIKGVMTSMLXXXXXXXXXXMEWVMALLL 354
               G + ++   + DV+L +Q+ E   Y+D  I+G+M  ML          MEW  +LLL
Sbjct: 282  ---GDEMKESRVMADVLLDLQQTEPHYYTDAFIRGMMQGMLSAGTDTSSATMEWAFSLLL 338

Query: 355  NHPNALKKAKAEIKEQVGHSHLIKDSDISKLHYLDNVIKETLRLFPAGPLLVAHESSQDC 534
            NHPN+L K + EI   VG + L+++SD+S L +L++V+KETLR++P  PLLV HESSQDC
Sbjct: 339  NHPNSLLKVQEEIDTHVGPNRLLQESDLSNLPFLNSVLKETLRIYPVAPLLVPHESSQDC 398

Query: 535  TVLGSHIPKGTMLLVNAYAMQRDNQLWDNPLEFKPERFDSDDHVLHGDEGYKYIPFGTGR 714
             V G H+P+GTMLLVN +A+Q D+  W +P EFKPERF     V   +EG +++PFG GR
Sbjct: 399  VVGGFHVPRGTMLLVNNWAIQNDSDSWPDPAEFKPERFQDAGEV---EEGLRWLPFGAGR 455

Query: 715  RRCPGESLAWKVMLLTLGALIQCFEWERVGKELVDLSEGVGLSLPMAKPLQAMYKP 882
            R CPGE LA +++ LTLG LIQCFEW RVG+E+VD+SEG GL++P A+PL A Y+P
Sbjct: 456  RGCPGEGLAMRMVGLTLGCLIQCFEWRRVGEEMVDMSEGGGLTMPRARPLWANYRP 511


>ref|XP_002327185.1| cytochrome P450 [Populus trichocarpa] gi|222835500|gb|EEE73935.1|
            cytochrome P450 [Populus trichocarpa]
          Length = 601

 Score =  283 bits (725), Expect = 3e-74
 Identities = 136/298 (45%), Positives = 200/298 (67%), Gaps = 4/298 (1%)
 Frame = +1

Query: 1    VEEVFLLSGASTLEDFIPVVRWLRIGGAEKRMERVGKELDEFYQKIIEDRRRVGKWKEY- 177
            ++E+  L G+S + DF PV++W+   G EKRM  + K++D+F Q +IE+ ++V       
Sbjct: 211  IKEMEALRGSSNMNDFFPVLQWIDFQGLEKRMMGLKKKMDKFLQDLIEEHQKVRSQSSQS 270

Query: 178  --VDGHDQEKKG-NIIDVMLAMQEKEKDNYSDVAIKGVMTSMLXXXXXXXXXXMEWVMAL 348
              + G   +K+   +IDVML+++E E + Y+D  IKGV+ S L          +EW M+L
Sbjct: 271  TKITGLGNQKRNMTLIDVMLSLKETEPEFYTDQTIKGVIMSTLTAGSQTSAATLEWAMSL 330

Query: 349  LLNHPNALKKAKAEIKEQVGHSHLIKDSDISKLHYLDNVIKETLRLFPAGPLLVAHESSQ 528
            LLN+P  ++KA  EI   VG  H++ + D++KL YL N++ ET RLFP  PLL+ HESS+
Sbjct: 331  LLNNPETMRKASEEIDAIVGTEHILDEVDVTKLSYLQNILNETFRLFPPAPLLLPHESSE 390

Query: 529  DCTVLGSHIPKGTMLLVNAYAMQRDNQLWDNPLEFKPERFDSDDHVLHGDEGYKYIPFGT 708
            DCT+ G H+P+GTMLLVN +++ RD +LW  P +F PERF+  +      EGYK +PFG 
Sbjct: 391  DCTISGFHVPRGTMLLVNTWSIHRDTKLWVEPTKFMPERFEGGE-----GEGYKLLPFGA 445

Query: 709  GRRRCPGESLAWKVMLLTLGALIQCFEWERVGKELVDLSEGVGLSLPMAKPLQAMYKP 882
            GRR CPG  LA +++ LTLG LIQCFEW+RV KE ++L+EG GL++P A+PL+A+ +P
Sbjct: 446  GRRACPGAGLAKRIIGLTLGVLIQCFEWDRVSKEEINLTEGTGLTIPKAEPLEALCRP 503



 Score = 69.7 bits (169), Expect = 8e-10
 Identities = 32/55 (58%), Positives = 43/55 (78%)
 Frame = +1

Query: 715 RRCPGESLAWKVMLLTLGALIQCFEWERVGKELVDLSEGVGLSLPMAKPLQAMYK 879
           R CPG  LA +V+ L L +LIQCFEWER+ +E VD+SEG G+++  AKPL+AM+K
Sbjct: 537 RSCPGAGLANRVVGLALASLIQCFEWERIREEEVDMSEGSGITMLKAKPLKAMFK 591


>gb|ADD84651.1| CYP81B36 [Scoparia dulcis]
          Length = 502

 Score =  283 bits (724), Expect = 4e-74
 Identities = 142/291 (48%), Positives = 202/291 (69%), Gaps = 1/291 (0%)
 Frame = +1

Query: 1    VEEVFLLSGASTLEDFIPVVRWLRIGGAEKRMERVGKELDEFYQKIIEDRRRVGKWKEYV 180
            V+E   L G S + DF+P+VRWL IGGAEK +  + ++ D F Q+++E+ +R        
Sbjct: 207  VKETLRLGGTS-MGDFLPIVRWLGIGGAEKGLAELQRKRDGFMQELVEECKR-----RIR 260

Query: 181  DGHDQEKKGNIIDVMLAMQEKEKDNYSDVAIKGVMTSMLXXXXXXXXXXMEWVMALLLNH 360
            +         +I+++L +QEKE + Y+D  I+ +M ++L          MEWV++LLLN+
Sbjct: 261  NSSGDSGPMTMIEMLLPLQEKEPEYYTDKLIRSLMLTLLVAGTDTTAGTMEWVVSLLLNN 320

Query: 361  PNALKKAKAEIKEQVGHSHLIKDSDISKLHYLDNVIKETLRLFPAGPLLVAHESSQDCTV 540
            P+ +KKA+ EI   +GH HLI +SDI+ L YL  ++ ETLRL+PAGPLLV HESS+ C V
Sbjct: 321  PHVIKKAQVEIDNHIGHGHLIDESDITDLPYLRCIVNETLRLYPAGPLLVPHESSEQCIV 380

Query: 541  LGSHIPKGTMLLVNAYAMQRDNQLWDNPLEFKPERFDSDDHVLHGD-EGYKYIPFGTGRR 717
             G  +P GTMLLVN +A+QRD + WD P +FKPERF+     L G+ +G+K+ PFG+GRR
Sbjct: 381  GGYRVPAGTMLLVNLWAIQRDPKYWDEPEKFKPERFEG----LEGNRDGFKFSPFGSGRR 436

Query: 718  RCPGESLAWKVMLLTLGALIQCFEWERVGKELVDLSEGVGLSLPMAKPLQA 870
             CPGE LA +++  ++G++IQCF+WERVGKELVD+SEGVGL+LP A+PL A
Sbjct: 437  GCPGEGLAVRMLASSIGSIIQCFDWERVGKELVDMSEGVGLTLPKAQPLMA 487


>dbj|BAF98467.1| cytochrome P450 [Coptis japonica var. dissecta]
          Length = 503

 Score =  281 bits (718), Expect = 2e-73
 Identities = 140/296 (47%), Positives = 201/296 (67%), Gaps = 2/296 (0%)
 Frame = +1

Query: 1    VEEVFLLSGASTLEDFIPVVRWLRIGGAEKRMERVGKELDEFYQKIIEDRR--RVGKWKE 174
            V+    +SGAS + DF+P++ W+ I G EK++  + ++ D+F Q++IE+ R  R G  + 
Sbjct: 206  VQGTLAISGASNIGDFLPILSWIGIKGIEKKVWNLKEKRDKFLQELIEEHRISRSGGMQT 265

Query: 175  YVDGHDQEKKGNIIDVMLAMQEKEKDNYSDVAIKGVMTSMLXXXXXXXXXXMEWVMALLL 354
                  +EK   +IDV+L++QE E + Y+D  I G++  +L          MEW M+LLL
Sbjct: 266  ------KEKNATLIDVLLSLQETEPEYYTDEMILGIIWVLLAAGTDTSAVTMEWAMSLLL 319

Query: 355  NHPNALKKAKAEIKEQVGHSHLIKDSDISKLHYLDNVIKETLRLFPAGPLLVAHESSQDC 534
            N+P  +KKA+AEI   +    LI +SD++KL YL ++I ETLR++PAGPLLV HESS++C
Sbjct: 320  NNPQVIKKAQAEIDNNLEQGRLINESDVNKLPYLHSIITETLRIYPAGPLLVPHESSEEC 379

Query: 535  TVLGSHIPKGTMLLVNAYAMQRDNQLWDNPLEFKPERFDSDDHVLHGDEGYKYIPFGTGR 714
             V G  +P GTMLLVN +A+Q+D  +W  P +FKPERFD  +      +G+K +PFG+GR
Sbjct: 380  IVGGYKVPSGTMLLVNVWAIQQDPNIWVEPTKFKPERFDGFEGT---RDGFKLMPFGSGR 436

Query: 715  RRCPGESLAWKVMLLTLGALIQCFEWERVGKELVDLSEGVGLSLPMAKPLQAMYKP 882
            R CPGE LA +V+ L LGALIQCF+WERVG+E+VD+SEG GL+LP   PL+A  +P
Sbjct: 437  RGCPGEGLAMRVVALALGALIQCFDWERVGEEMVDMSEGPGLTLPKVHPLEAKCRP 492