BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dioscorea21_contig00008497
         (2756 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002271798.1| PREDICTED: isoamylase 2, chloroplastic [Viti...   967   0.0  
ref|XP_002533079.1| isoamylase, putative [Ricinus communis] gi|2...   927   0.0  
ref|XP_003554240.1| PREDICTED: isoamylase 2, chloroplastic-like ...   881   0.0  
ref|XP_004164565.1| PREDICTED: LOW QUALITY PROTEIN: isoamylase 2...   863   0.0  
ref|XP_004136188.1| PREDICTED: isoamylase 2, chloroplastic-like ...   862   0.0  

>ref|XP_002271798.1| PREDICTED: isoamylase 2, chloroplastic [Vitis vinifera]
          Length = 882

 Score =  967 bits (2500), Expect = 0.0
 Identities = 485/837 (57%), Positives = 600/837 (71%), Gaps = 5/837 (0%)
 Frame = +2

Query: 14   NATIICKGIQNFKTSAAPTKSIDQLEQVISDTD-EELGKVASYLFRTEXXXXXXXXXXXX 190
            NAT  C    N+K SA    +I++ E   S T+ EEL    +YLF TE            
Sbjct: 58   NATRNCFRNINWKVSATSHIAIEETENRYSTTESEELESRLNYLFLTEIGGQVKVIVGKK 117

Query: 191  SIKYSVIVEVSSLPNFTSDHKLVMCWGIFRSDSACFLVPDHESLASETTKLSDESRLDSF 370
            + KY V +EVSSL  + SD+KL++ WG+FRS+S+CF+  D ++L  E    + E      
Sbjct: 118  NKKYIVSIEVSSLQLYNSDNKLILSWGVFRSNSSCFMPVDFQNLVPEVGSNTTE------ 171

Query: 371  RTPLSQNSSGIYTAKLEFDSAQIPFYVTFSLXXXXXXXXTESEIRTHQKTSFCVPVGIGR 550
              P  + SSG +  KL+F++   PFY++F L        + S+IR+H+KT+FC+PVG GR
Sbjct: 172  -IPFMERSSGSFALKLDFEANHAPFYLSFLLKSTLDTDLSSSDIRSHRKTNFCIPVGFGR 230

Query: 551  GHPLPLGVSFSDDNLMNFSLFSRNAEGVVLCLYDGTTNQPALEIELDPYVNRTGDIWHIS 730
            G+P PLG+SFS D   NF+ FSRNA GVVLCLYDGT+++PALEI+LDPYVNRTGDIWH S
Sbjct: 231  GYPAPLGLSFSSDGSPNFAFFSRNAGGVVLCLYDGTSDKPALEIDLDPYVNRTGDIWHAS 290

Query: 731  MEQTGKYVKYGYRCKGSVLWEIGSRFHTRHVLLDPYAKKIGKISFNESQSVSSVKYLGCL 910
            ME  G +V YGYRCK + L + G   HT HV LDPYAK I + SF++   +     LG L
Sbjct: 291  MESVGSFVSYGYRCKEANLQDSGETLHTEHVHLDPYAKLI-RNSFSDDHGLKPQPRLGEL 349

Query: 911  DDEPAFDWNGDVHPMLPMEKMAVYRLNICNFTEDKSSGLSKNVAGTFSGVIHKLQHIKSL 1090
              EPAF+WN DVHP +PMEK+ VYRLN+ +FT+D+SS ++ ++AGTFSG++ KL H K L
Sbjct: 350  QKEPAFNWNDDVHPYIPMEKLVVYRLNVMHFTKDESSQVASDLAGTFSGLMEKLHHFKDL 409

Query: 1091 GFNAILFEPIFSFDEQKGPYYPFHFFSVMNSCGREGNLELANNSVKEMIKTLHENGMEVL 1270
            G NA+L EPIFSFDEQKGPY+PFHFFS MN  G         NSVKEM+K LH NG+EVL
Sbjct: 410  GVNAVLLEPIFSFDEQKGPYFPFHFFSPMNVYGPSSGPVSTINSVKEMVKRLHANGIEVL 469

Query: 1271 LEVVFSHTSEGGDAACQTTSFRGIDNSSYYIVEGDIGSGINNALKCNSPVVQQMILDSLR 1450
            LEVVF+HT+E G       + +GID+S YY V GD   GI NAL CN  +VQQMI+DSLR
Sbjct: 470  LEVVFTHTAESG-------ALQGIDDSCYYYVNGDADLGIRNALNCNYSIVQQMIVDSLR 522

Query: 1451 HWVTDFHVDGFCFINAAHLLRGVNGKYLSRPPLVEAIAFDPLLSKTKIIADCWSPLDLSF 1630
            +WVT+FHVDGFCFINA+ LLRG +G+YLSRPPLVE IAFDPLLSKTKIIADCW P ++  
Sbjct: 523  YWVTEFHVDGFCFINASSLLRGFHGEYLSRPPLVETIAFDPLLSKTKIIADCWDPRNMLP 582

Query: 1631 MKIDFPHWKRWAEMNGNFCVDVRNFVRGQGSLSDLATRLCGSGDVFSDSRGPAFSFNFIA 1810
             +I FPHWKRWAE+N  FC DVRNF+RG+G LSD ATRLCGSGD+F D RGPAFSFNF  
Sbjct: 583  KEIRFPHWKRWAEVNTRFCNDVRNFLRGEG-LSDFATRLCGSGDIFMDGRGPAFSFNFTT 641

Query: 1811 RNSGLPLVDLVSFSDGDLALELSWNCGAEGPTNNSLILGTRLKQIRNFLFILFVSLGVPV 1990
            +N GLPLVDLVSFS  +LA ELSWNCG EGPTN + +L  RLKQIRNFLFIL+VSLGVP+
Sbjct: 642  KNFGLPLVDLVSFSSSELASELSWNCGDEGPTNKTTVLERRLKQIRNFLFILYVSLGVPI 701

Query: 1991 LNMGDEFGYSADGLTLHGDRKPLNWSYLKTGFGSQIMQFIAFLNSLRTRRSDLFQKNDFL 2170
            LNMGDE G S+ G   +GDRKP NW+ +KTGFG Q +QFI+FL+SLR+RRSDL Q+  FL
Sbjct: 702  LNMGDECGQSSGGSPAYGDRKPFNWNSVKTGFGIQTIQFISFLSSLRSRRSDLLQRRSFL 761

Query: 2171 NAENIKWHGSNDSPPKWDDPSCKFLAMTLKAEKHE----TTPSSSNNDIFVSFNASNHRD 2338
              E+I WHGS+ SPP+WDDPS KFLAMTLKAE  E    +  SS   D+F++FN ++   
Sbjct: 762  KEESIDWHGSDQSPPRWDDPSSKFLAMTLKAENMEGQLPSESSSIKGDLFIAFNTADRSV 821

Query: 2339 IVVLPEVSEGTIWRRLVDTALPFPGFFSDISDSDPLELAGLSEYRMGSHSCVLFEAS 2509
             V+LP    G +W RLVDTALPFPGFF+   ++   + +GL  Y+M SHSC LFEA+
Sbjct: 822  KVILPPPPTGMVWHRLVDTALPFPGFFTADGEAILKKKSGLVTYKMESHSCALFEAN 878


>ref|XP_002533079.1| isoamylase, putative [Ricinus communis] gi|223527143|gb|EEF29318.1|
            isoamylase, putative [Ricinus communis]
          Length = 872

 Score =  927 bits (2395), Expect = 0.0
 Identities = 459/803 (57%), Positives = 578/803 (71%), Gaps = 5/803 (0%)
 Frame = +2

Query: 113  EELGKVASYLFRTEXXXXXXXXXXXXSIKYSVIVEVSSLPNFTSDHKLVMCWGIFRSDSA 292
            +EL KV++YLFRT+            + KY+V +EVSSL   T+D++L++ WGI+RSDS+
Sbjct: 84   DELNKVSTYLFRTQFGGHVKVLVRKKNAKYAVYIEVSSLELGTTDYRLMLIWGIYRSDSS 143

Query: 293  CFLVPDHESLASETTKLSDESRLDSFRTPLSQNSSGIYTAKLEFDSAQIPFYVTFSLXXX 472
            CF+  D ++ A    K+          T L QNS G +  +LEF+  Q PFY++F L   
Sbjct: 144  CFMPLDSQNFAPNARKMD---------TALVQNSFGTFALELEFEPKQTPFYLSFLLKSK 194

Query: 473  XXXXXTESEIRTHQKTSFCVPVGIGRGHPLPLGVSFSDDNLMNFSLFSRNAEGVVLCLYD 652
                 +  EI+ H+  +FCVP+G   G P PLG+SFS D  MNF+ FSRN EG+VLCLYD
Sbjct: 195  LNTDASGLEIKNHKNANFCVPIGFNSGDPSPLGLSFSTDGSMNFAFFSRNVEGLVLCLYD 254

Query: 653  -GTTNQPALEIELDPYVNRTGDIWHISMEQTGKYVKYGYRCKGSVLWEIGSRFHTRHVLL 829
              TT++PALE++LDPYVNRTGD+WH S+E    +  YGYRCKG++L    S+     VLL
Sbjct: 255  DSTTDKPALELDLDPYVNRTGDVWHASLEGAWTFTSYGYRCKGAILQGNTSKVDMECVLL 314

Query: 830  DPYAKKIGKISFNESQSVSSVKYLGCLDDEPAFDWNGDVHPMLPMEKMAVYRLNICNFTE 1009
            DPYA+ I   S  +  S  S KYLG L +EPAF+W  D+ P L MEK+ VYRLN+  FTE
Sbjct: 315  DPYARVIAS-SMTDHGSRLSAKYLGRLCEEPAFEWGSDIRPNLAMEKLIVYRLNVKRFTE 373

Query: 1010 DKSSGLSKNVAGTFSGVIHKLQHIKSLGFNAILFEPIFSFDEQKGPYYPFHFFSVMNSCG 1189
             KS  L  ++AGTF+G+I K+ H ++LG NA+L EPIF FDEQKGPY+P+HFFS  N  G
Sbjct: 374  HKSGKLYSDIAGTFAGLIEKMDHFRNLGVNAVLLEPIFPFDEQKGPYFPYHFFSPSNIYG 433

Query: 1190 REGNLELANNSVKEMIKTLHENGMEVLLEVVFSHTSEGGDAACQTTSFRGIDNSSYYIVE 1369
              G    A  S+KEM+K LH N +EVLLEVVF+HT+EGG       + +GID+ SYY  +
Sbjct: 434  PSGGSISAITSMKEMVKELHANRIEVLLEVVFTHTAEGG-------ALQGIDDFSYYYTK 486

Query: 1370 GDIGSGINNALKCNSPVVQQMILDSLRHWVTDFHVDGFCFINAAHLLRGVNGKYLSRPPL 1549
              + S   NAL CN P+VQ+MILDSL+HWVT+FH+DGFCFINA+ LL G +G++LSRPPL
Sbjct: 487  SSMDS--RNALNCNYPIVQRMILDSLQHWVTEFHIDGFCFINASALLTGFHGEHLSRPPL 544

Query: 1550 VEAIAFDPLLSKTKIIADCWSPLDLSFMKIDFPHWKRWAEMNGNFCVDVRNFVRGQGSLS 1729
            VEAIAFDP+LSKTKIIAD W P      +  FPHWKRWAE+N  FC+DVRNF+RG+  L 
Sbjct: 545  VEAIAFDPILSKTKIIADPWHPEHRIPKETCFPHWKRWAEINPKFCIDVRNFLRGESLLG 604

Query: 1730 DLATRLCGSGDVFSDSRGPAFSFNFIARNSGLPLVDLVSFSDGDLALELSWNCGAEGPTN 1909
            DLATRLCGSGD+FS+ RGPAFSFN+IARNSGLPLVDLVSFS G+L  ELSWNCG EGPTN
Sbjct: 605  DLATRLCGSGDIFSNGRGPAFSFNYIARNSGLPLVDLVSFSGGELGSELSWNCGEEGPTN 664

Query: 1910 NSLILGTRLKQIRNFLFILFVSLGVPVLNMGDEFGYSADGLTLHGDRKPLNWSYLKTGFG 2089
             + +L  RLKQIRN+LFIL+VSLGVPVLNMGDE G S+ G   +GDRKP +W+ L T FG
Sbjct: 665  KTAVLERRLKQIRNYLFILYVSLGVPVLNMGDECGQSSRGSISYGDRKPFDWNALSTSFG 724

Query: 2090 SQIMQFIAFLNSLRTRRSDLFQKNDFLNAENIKWHGSNDSPPKWDDPSCKFLAMTLKAEK 2269
            +Q+ QFI+FL+SLR RRSDL QK +FL  ENI WHG++ SPP+W+DP+CKFLAMTLK +K
Sbjct: 725  NQMTQFISFLSSLRMRRSDLLQKRNFLKEENIDWHGNDQSPPRWEDPTCKFLAMTLKIDK 784

Query: 2270 HE----TTPSSSNNDIFVSFNASNHRDIVVLPEVSEGTIWRRLVDTALPFPGFFSDISDS 2437
             E    + PS+   D+F++FNA+ H + V+LP V EG IWRRLVDTALPFPGFFS+  + 
Sbjct: 785  AESQLSSEPSNIKGDLFMAFNAAGHAESVILPPVPEGMIWRRLVDTALPFPGFFSEDGEP 844

Query: 2438 DPLELAGLSEYRMGSHSCVLFEA 2506
               ++AGL  Y+M SHSC LFEA
Sbjct: 845  VVEQIAGLIAYKMNSHSCTLFEA 867


>ref|XP_003554240.1| PREDICTED: isoamylase 2, chloroplastic-like [Glycine max]
          Length = 865

 Score =  881 bits (2277), Expect = 0.0
 Identities = 460/830 (55%), Positives = 575/830 (69%), Gaps = 9/830 (1%)
 Frame = +2

Query: 50   KTSAAPTKSIDQLEQVISDTD--EELGKVASYLFRTEXXXXXXXXXXXX-SIKYSVIVEV 220
            K  A    SI++ EQ I      E+L    +YLFRTE             + +YSV +E+
Sbjct: 56   KLCATSRLSIEETEQQIGTLTRPEDLKGSLAYLFRTETGGGLVKVHVTRRNDRYSVYIEI 115

Query: 221  SSLP-NFTSDHKLVMCWGIFRSDSACFLVPDHESLASETTKLSDESRLDSFRTPLSQNSS 397
            SSL  +      L++CWG++RSDS+CF+  D       T  LS+ + +    +PL QNS 
Sbjct: 116  SSLDISGGVGEALLLCWGVYRSDSSCFVDLD-------TIGLSENAAMGMNVSPLVQNSD 168

Query: 398  GIYTAKLEFDSAQIPFYVTFSLXXXXXXXXTESEIRTHQKTSFCVPVGIGRGHPLPLGVS 577
            G +  +LEFD+  +P Y++F L           EIR+H++T+FC+PVG   G+P PLGVS
Sbjct: 169  GKFAIELEFDAKHVPLYLSFFLMSSLDSGL---EIRSHRRTNFCMPVGSLPGYPCPLGVS 225

Query: 578  FSDDNLMNFSLFSRNAEGVVLCLYDGT-TNQPALEIELDPYVNRTGDIWHISMEQTGKYV 754
            +S D  +NFS+FSR+AE VVLCLYD     +PALE++LDPYVNRTGDIWH+S E    +V
Sbjct: 226  YSPDGSVNFSIFSRHAESVVLCLYDENGVEKPALELDLDPYVNRTGDIWHVSFESAKGFV 285

Query: 755  KYGYRCKGSVLWEIGSRFHTRHVLLDPYAKKIGKISFNESQSVSSVKYLGCLDDEPAFDW 934
             YGYRC+  VL +        HV+LDPYAK +G    +    V  VK LGCL  EP FDW
Sbjct: 286  SYGYRCRRGVLKKNKDDGFAEHVVLDPYAKIVGN---SYPDGVGLVKNLGCLRKEPFFDW 342

Query: 935  NGDVHPMLPMEKMAVYRLNICNFTEDKSSGLSKNVAGTFSGVIHKLQHIKSLGFNAILFE 1114
             GD HP L MEK+ VYRLN+  FT+ +SS L   +AGTF+G+  K+QH K LG NA+L E
Sbjct: 343  GGDRHPDLSMEKLVVYRLNVKRFTQHESSQLPSGLAGTFTGLAKKVQHFKDLGVNAVLLE 402

Query: 1115 PIFSFDEQKGPYYPFHFFSVMNSCGREGNLELANNSVKEMIKTLHENGMEVLLEVVFSHT 1294
            P+F+FDE+KGPY+P HFFS+M+  G  G    A  ++KEM+KT+H NG+EVL+EVVFS+T
Sbjct: 403  PVFTFDEKKGPYFPCHFFSLMHIYGPSGGPVSAIAAMKEMVKTMHANGIEVLVEVVFSNT 462

Query: 1295 SEGGDAACQTTSFRGIDNSSYYIVEGDIGSGINNALKCNSPVVQQMILDSLRHWVTDFHV 1474
            +E G       + +GID+SSYY   G  G  + +AL CN P+VQ +ILDSLRHWVT+FH+
Sbjct: 463  AEIG-------AIQGIDDSSYYYANGVGGLKVQSALNCNYPIVQNLILDSLRHWVTEFHI 515

Query: 1475 DGFCFINAAHLLRGVNGKYLSRPPLVEAIAFDPLLSKTKIIADCWSPLDLSFMKIDFPHW 1654
            DGF FINA+HLLRG +G+YLSRPPLVEAIAFDP+LSKTKIIADCW P  +   +I FPHW
Sbjct: 516  DGFSFINASHLLRGFHGEYLSRPPLVEAIAFDPVLSKTKIIADCWDPHGMVAKEIRFPHW 575

Query: 1655 KRWAEMNGNFCVDVRNFVRGQGSLSDLATRLCGSGDVFSDSRGPAFSFNFIARNSGLPLV 1834
             RWAEMN +FC DVRNF+RGQ  LSDLATRLCGSGD+FS  RGP FSFN+IARN G+ LV
Sbjct: 576  MRWAEMNTHFCNDVRNFLRGQNLLSDLATRLCGSGDIFSGGRGPGFSFNYIARNFGVSLV 635

Query: 1835 DLVSFSDGDLALELSWNCGAEGPTNNSLILGTRLKQIRNFLFILFVSLGVPVLNMGDEFG 2014
            DLVSFS  D   ELSWNCGAEGPTNN+ +L  RLKQIRNFLFILFVSLGVPVLNMGDE G
Sbjct: 636  DLVSFSSVD---ELSWNCGAEGPTNNTAVLERRLKQIRNFLFILFVSLGVPVLNMGDECG 692

Query: 2015 YSADGLTLHGDRKPLNWSYLKTGFGSQIMQFIAFLNSLRTRRSDLFQKNDFLNAENIKWH 2194
             S+ G T +   KP +WS LKTGFG Q  +FI FL+SLR RRS L Q+  FL  ENI+W+
Sbjct: 693  QSSGGFTAYDGIKPFSWSALKTGFGKQTSEFIFFLSSLRKRRSYLLQRRSFLKEENIEWY 752

Query: 2195 GSNDSPPKWDDPSCKFLAMTLKAEKHETTPSSSNNDI----FVSFNASNHRDIVVLPEVS 2362
            GS+  PP+W+DPSCKFLAM LKAE  E   SS ++DI    F++FNA++H +  VLP   
Sbjct: 753  GSDGDPPRWEDPSCKFLAMILKAEVTEFLESSVSSDISGDLFIAFNATDHPETAVLPLPP 812

Query: 2363 EGTIWRRLVDTALPFPGFFSDISDSDPLELAGLSEYRMGSHSCVLFEASD 2512
            EG  W RLVDTALPFPGFFS   +  P +  GL  Y++ S+SC LFEA++
Sbjct: 813  EGMSWYRLVDTALPFPGFFSTSGEVVPEQTEGLFTYQVKSYSCTLFEANN 862


>ref|XP_004164565.1| PREDICTED: LOW QUALITY PROTEIN: isoamylase 2, chloroplastic-like
            [Cucumis sativus]
          Length = 885

 Score =  863 bits (2229), Expect = 0.0
 Identities = 438/810 (54%), Positives = 558/810 (68%), Gaps = 7/810 (0%)
 Frame = +2

Query: 98   ISDTDEELGKVASYLFRTEXXXXXXXXXXXXSIKYSVIVEVSSLPNFTSDHKLVMCWGIF 277
            I    +E  +VA+YLFRTE               ++V +EV S+   + D  L++ WG++
Sbjct: 88   IGGKSKEQRRVATYLFRTEFGDLVNVFVGKKGSTFTVNIEVPSMQLVSIDEALLLSWGVY 147

Query: 278  RSDSACFLVPDHESLASETTKLSDESRLDSFRTPLSQNSSGIYTAKLEFDSAQIPFYVTF 457
            RSDSA  + P+ ES   + T  + E       TP  + S G ++ +LEFD+   PFY++F
Sbjct: 148  RSDSA-LVTPNFESSPPDETTGATE-------TPFVKTSEGKFSVELEFDAKHTPFYLSF 199

Query: 458  SLXXXXXXXXTESEIRTHQKTSFCVPVGIGRGHPLPLGVSFSDDNLMNFSLFSRNAEGVV 637
             L          SEIR+H+KTSF VPVG GRG+P PLG+S S D  +NFS+FS +AE +V
Sbjct: 200  VLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSISGDGSVNFSIFSSSAESLV 259

Query: 638  LCLY-DGTTNQPALEIELDPYVNRTGDIWHISMEQTGKYVKYGYRCKGSVLWEIGSRFHT 814
            LCLY D T+ +P LE++LDPY+NR+G+IWH S E   K+V YGY+CKGS   E       
Sbjct: 260  LCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVSYGYQCKGSKSHENQDGLEV 319

Query: 815  RHVLLDPYAKKIG-KISFNESQSVS-SVKYLGCLDDEPAFDWNGDVHPMLPMEKMAVYRL 988
              +++DPYAK +   I  +  Q +    K+LG +   P FDW+G+VHP LPMEK+ VYRL
Sbjct: 320  SRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFDWDGEVHPNLPMEKLXVYRL 379

Query: 989  NICNFTEDKSSGLSKNVAGTFSGVIHKLQHIKSLGFNAILFEPIFSFDEQKGPYYPFHFF 1168
            N+  FT DKSS L  ++AGTFSG+  KL H K+LG NA+L EPIF FDE++GPY+PFHFF
Sbjct: 380  NVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLLEPIFQFDEKEGPYFPFHFF 439

Query: 1169 SVMNSCGREGNLELANNSVKEMIKTLHENGMEVLLEVVFSHTSEGGDAACQTTSFRGIDN 1348
            S  N+ G  G    A NS+KEM+K LH NG+EV+LEVV++HTS  G       + +GID+
Sbjct: 440  SPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTHTSGNG-------ALQGIDD 492

Query: 1349 SSYYIVEGDIGSGINNALKCNSPVVQQMILDSLRHWVTDFHVDGFCFINAAHLLRGVNGK 1528
            SSYY           +AL CN P+VQQ++LDSLR+WVT+FHVDGFCF+NA+ LLRG +G+
Sbjct: 493  SSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFVNASFLLRGHHGE 552

Query: 1529 YLSRPPLVEAIAFDPLLSKTKIIADCWSPLDLSFMKIDFPHWKRWAEMNGNFCVDVRNFV 1708
             LSRPP VEAIAFDPLLSKTK++AD W P +L   +  FPHWKRWAE+N  FC D+R+F 
Sbjct: 553  LLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEVNSKFCSDIRDFF 612

Query: 1709 RGQGSLSDLATRLCGSGDVFSDSRGPAFSFNFIARNSGLPLVDLVSFSDGDLALELSWNC 1888
            RG+G +S LATRLCGSGDVFSD RGPAFSFNFIARN GLPLVDLVSFS+ +LA ELSWNC
Sbjct: 613  RGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFSNSNLASELSWNC 672

Query: 1889 GAEGPTNNSLILGTRLKQIRNFLFILFVSLGVPVLNMGDEFGYSADGLTLHGDRKPLNWS 2068
            G EGPT+N  +L  RLKQIRNF+F+LFVSLGVPVLNMGDE G S+ G     D++  NW 
Sbjct: 673  GEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGSVAFNDKRSFNWD 732

Query: 2069 YLKTGFGSQIMQFIAFLNSLRTRRSDLFQKNDFLNAENIKWHGSNDSPPKWDDPSCKFLA 2248
             LKT FG+Q  QFIAFL+S R+RR DLFQ  +FL  ENI W  +N SPP+W+D SCKFLA
Sbjct: 733  LLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSPPQWEDASCKFLA 792

Query: 2249 MTLKAEKHETTPSSSN----NDIFVSFNASNHRDIVVLPEVSEGTIWRRLVDTALPFPGF 2416
            + L+A+K E    + N    ++IF+ FNAS+  + V LPE  EGT W R+VDTALPFPGF
Sbjct: 793  VMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWFRVVDTALPFPGF 852

Query: 2417 FSDISDSDPLELAGLSEYRMGSHSCVLFEA 2506
            FS  SD + + + G   Y + +HSC LFEA
Sbjct: 853  FS--SDGELVPMTGSVTYEIQAHSCALFEA 880


>ref|XP_004136188.1| PREDICTED: isoamylase 2, chloroplastic-like [Cucumis sativus]
          Length = 885

 Score =  862 bits (2228), Expect = 0.0
 Identities = 438/810 (54%), Positives = 558/810 (68%), Gaps = 7/810 (0%)
 Frame = +2

Query: 98   ISDTDEELGKVASYLFRTEXXXXXXXXXXXXSIKYSVIVEVSSLPNFTSDHKLVMCWGIF 277
            I    +E  +VA+YLFRTE               ++V +EV S+   + D  L++ WG++
Sbjct: 88   IGGKSKEQRRVATYLFRTEFGDLVNVFVGKKGSTFTVNIEVPSMQLVSIDEALLLSWGVY 147

Query: 278  RSDSACFLVPDHESLASETTKLSDESRLDSFRTPLSQNSSGIYTAKLEFDSAQIPFYVTF 457
            RSDSA  + P+ ES   + T  + E       TP  + S G ++ +LEFD+   PFY++F
Sbjct: 148  RSDSA-LVTPNFESSPPDETTGATE-------TPFVKTSEGKFSVELEFDAKHTPFYLSF 199

Query: 458  SLXXXXXXXXTESEIRTHQKTSFCVPVGIGRGHPLPLGVSFSDDNLMNFSLFSRNAEGVV 637
             L          SEIR+H+KTSF VPVG GRG+P PLG+S S D  +NFS+FS +AE +V
Sbjct: 200  VLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSISGDGSVNFSIFSSSAESLV 259

Query: 638  LCLY-DGTTNQPALEIELDPYVNRTGDIWHISMEQTGKYVKYGYRCKGSVLWEIGSRFHT 814
            LCLY D T+ +P LE++LDPY+NR+G+IWH S E   K+V YGY+CKGS   E       
Sbjct: 260  LCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVSYGYQCKGSKSHENQDGLEV 319

Query: 815  RHVLLDPYAKKIG-KISFNESQSVS-SVKYLGCLDDEPAFDWNGDVHPMLPMEKMAVYRL 988
              +++DPYAK +   I  +  Q +    K+LG +   P FDW+G+VHP LPMEK+ VYRL
Sbjct: 320  SRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFDWDGEVHPNLPMEKLFVYRL 379

Query: 989  NICNFTEDKSSGLSKNVAGTFSGVIHKLQHIKSLGFNAILFEPIFSFDEQKGPYYPFHFF 1168
            N+  FT DKSS L  ++AGTFSG+  KL H K+LG NA+L EPIF FDE++GPY+PFHFF
Sbjct: 380  NVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLLEPIFQFDEKEGPYFPFHFF 439

Query: 1169 SVMNSCGREGNLELANNSVKEMIKTLHENGMEVLLEVVFSHTSEGGDAACQTTSFRGIDN 1348
            S  N+ G  G    A NS+KEM+K LH NG+EV+LEVV++HTS  G       + +GID+
Sbjct: 440  SPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTHTSGNG-------ALQGIDD 492

Query: 1349 SSYYIVEGDIGSGINNALKCNSPVVQQMILDSLRHWVTDFHVDGFCFINAAHLLRGVNGK 1528
            SSYY           +AL CN P+VQQ++LDSLR+WVT+FHVDGFCF+NA+ LLRG +G+
Sbjct: 493  SSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFVNASFLLRGHHGE 552

Query: 1529 YLSRPPLVEAIAFDPLLSKTKIIADCWSPLDLSFMKIDFPHWKRWAEMNGNFCVDVRNFV 1708
             LSRPP VEAIAFDPLLSKTK++AD W P +L   +  FPHWKRWAE+N  FC D+R+F 
Sbjct: 553  LLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEVNSKFCSDIRDFF 612

Query: 1709 RGQGSLSDLATRLCGSGDVFSDSRGPAFSFNFIARNSGLPLVDLVSFSDGDLALELSWNC 1888
            RG+G +S LATRLCGSGDVFSD RGPAFSFNFIARN GLPLVDLVSFS+ +LA ELSWNC
Sbjct: 613  RGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFSNSNLASELSWNC 672

Query: 1889 GAEGPTNNSLILGTRLKQIRNFLFILFVSLGVPVLNMGDEFGYSADGLTLHGDRKPLNWS 2068
            G EGPT+N  +L  RLKQIRNF+F+LFVSLGVPVLNMGDE G S+ G     D++  NW 
Sbjct: 673  GEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGSVAFNDKRSFNWD 732

Query: 2069 YLKTGFGSQIMQFIAFLNSLRTRRSDLFQKNDFLNAENIKWHGSNDSPPKWDDPSCKFLA 2248
             LKT FG+Q  QFIAFL+S R+RR DLFQ  +FL  ENI W  +N SPP+W+D SCKFLA
Sbjct: 733  LLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSPPQWEDASCKFLA 792

Query: 2249 MTLKAEKHETTPSSSN----NDIFVSFNASNHRDIVVLPEVSEGTIWRRLVDTALPFPGF 2416
            + L+A+K E    + N    ++IF+ FNAS+  + V LPE  EGT W R+VDTALPFPGF
Sbjct: 793  VMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWFRVVDTALPFPGF 852

Query: 2417 FSDISDSDPLELAGLSEYRMGSHSCVLFEA 2506
            FS  SD + + + G   Y + +HSC LFEA
Sbjct: 853  FS--SDGELVPMTGSVTYEIQAHSCALFEA 880