BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dioscorea21_contig00006859
         (1807 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_003557748.1| PREDICTED: 3beta-hydroxysteroid-dehydrogenas...   652   0.0  
ref|XP_002282161.2| PREDICTED: 3beta-hydroxysteroid-dehydrogenas...   650   0.0  
ref|NP_001050327.1| Os03g0405000 [Oryza sativa Japonica Group] g...   649   0.0  
emb|CBI20743.3| unnamed protein product [Vitis vinifera]              650   0.0  
tpg|DAA45720.1| TPA: sterol-4-alpha-carboxylate 3-dehydrogenase,...   640   0.0  

>ref|XP_003557748.1| PREDICTED: 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform
            2-like [Brachypodium distachyon]
          Length = 560

 Score =  652 bits (1681), Expect(2) = 0.0
 Identities = 317/455 (69%), Positives = 378/455 (83%)
 Frame = -3

Query: 1805 RVKIMDLGPSIKLEPREEEGIIGQSLASGDAVYASADLRNKAQVIKAFEGAEVIFHMAAP 1626
            RV++ DL P+I L+  EEEGI+G +L  G AVYASADLR+KAQV  AFEGAEV+FHMAAP
Sbjct: 39   RVRVADLSPAIALDRDEEEGILGAALREGQAVYASADLRDKAQVANAFEGAEVVFHMAAP 98

Query: 1625 DSSINNYQLHYSVNVEGMKNVIDACIECKVKRLICTSSPSVVFDGVHGLFNVDESMPYPE 1446
            DSSINN+ LHYSVNVEG KNVIDACI CKVKRLI TSSPSVVFDGVHG+FN DESMPYP+
Sbjct: 99   DSSINNFHLHYSVNVEGTKNVIDACIRCKVKRLIYTSSPSVVFDGVHGIFNADESMPYPD 158

Query: 1445 KFNDAYSETKAEGEKLALKANGRNGLLTCCIRPSSIFGPGDRLLVPSLIAAAKAGKSKFI 1266
            KFND+YSETKA+ EKL ++ANGR+GLLTCCIRPSSIFGPGD+LLVPSL+AAA+AGKSK+I
Sbjct: 159  KFNDSYSETKADAEKLVIRANGRDGLLTCCIRPSSIFGPGDKLLVPSLVAAARAGKSKYI 218

Query: 1265 IGDGNNEYDFTYVENVAYAHVCAERTLASGGLSAEKAAGQAYFITNMEPIKFWEFMSRIL 1086
            IGDGNN YDFTYVENVAY HVCA++TL+S    A++AAG+AYFITN+EPIKFWEFMS IL
Sbjct: 219  IGDGNNYYDFTYVENVAYGHVCADKTLSSED-GAKRAAGKAYFITNVEPIKFWEFMSLIL 277

Query: 1085 EGLGYERPRMKIPASVIMPIAHLVERTYKMLAQYGMRVPQLTPSRVRLLSCNRTFNCSKA 906
            EGLGY+RP +KIP SV+MP+AH+VE TYK   +YGM+VPQLTPSR+RLLSCNRTF+CS+A
Sbjct: 278  EGLGYKRPSIKIPVSVMMPVAHVVELTYKTFCKYGMKVPQLTPSRIRLLSCNRTFSCSRA 337

Query: 905  KSLLGYEPVVSLEDGLKRTVESYPQLRAEEKTRMLSKASAYLGNGNIANALLWKDKKQXX 726
            K  LGYEP+VSL+DGLKRT+ESY  L+A+ + R +SKAS  LGNGNIA  LLW+D KQ  
Sbjct: 338  KDQLGYEPIVSLKDGLKRTIESYSHLQAQNQ-RSVSKASILLGNGNIAKTLLWEDTKQTM 396

Query: 725  XXXXXXXXXXXXXXTSGFTFIKATAKLLSVVALFLFIHGMLPEKIFSYKIEKISPTYFHL 546
                          T G+T I A AKL S+ ALFLFIHG+LP  +F +KIEK+ P+ FH+
Sbjct: 397  TVLLLLAVIYYQLFTCGYTIITAMAKLFSLTALFLFIHGLLPANVFGHKIEKLEPSNFHI 456

Query: 545  SEEKSQCLAYSVTSSWNSAIGIVRSLCRGKDWPLF 441
            S+ ++  +A SV SSWNS +G+++SLCRG DWPLF
Sbjct: 457  SQMEAHHVACSVRSSWNSLVGMLKSLCRGNDWPLF 491



 Score = 55.8 bits (133), Expect(2) = 0.0
 Identities = 27/51 (52%), Positives = 35/51 (68%)
 Frame = -1

Query: 358 KVFFMLLVLSFLASMSFQCIFKLAIPFLFIAFIVYEKWEDEIDGVVHNILS 206
           KV F LL++S L+SMS Q  FK+ IP +FI F  YEK+ED ID +V +  S
Sbjct: 493 KVVFFLLIVSILSSMSSQAAFKIGIPLIFIGFKAYEKFEDTIDSLVGDACS 543


>ref|XP_002282161.2| PREDICTED: 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform
            2-like [Vitis vinifera]
          Length = 563

 Score =  650 bits (1676), Expect(2) = 0.0
 Identities = 319/457 (69%), Positives = 376/457 (82%), Gaps = 2/457 (0%)
 Frame = -3

Query: 1802 VKIMDLGPSIKLEPREEEGIIGQSLASGDAVYASADLRNKAQVIKAFEGAEVIFHMAAPD 1623
            V+I DLGPSIKLEP EE+G++G++L SG A Y SADLR+KAQV+KA +GAE +FHMAAPD
Sbjct: 33   VRIADLGPSIKLEPSEEKGLLGEALQSGRAEYVSADLRDKAQVLKACQGAEAVFHMAAPD 92

Query: 1622 SSINNYQLHYSVNVEGMKNVIDACIECKVKRLICTSSPSVVFDGVHGLFNVDESMPYPEK 1443
            SSINN+QLH+SVNV+G KNVIDACIE KVKRLI TSSPSVVFDGVHG+FN DES+PYP+K
Sbjct: 93   SSINNHQLHHSVNVQGTKNVIDACIEQKVKRLIYTSSPSVVFDGVHGIFNGDESLPYPDK 152

Query: 1442 FNDAYSETKAEGEKLALKANGRNGLLTCCIRPSSIFGPGDRLLVPSLIAAAKAGKSKFII 1263
             ND+YS TKAEGE L +K+NG NGLLTCCIRPSSIFGPGD+LLVPSL+ AA+AGKSKFII
Sbjct: 153  HNDSYSATKAEGEALVIKSNGTNGLLTCCIRPSSIFGPGDKLLVPSLVNAARAGKSKFII 212

Query: 1262 GDGNNEYDFTYVENVAYAHVCAERTLASGGLSAEKAAGQAYFITNMEPIKFWEFMSRILE 1083
            GDGNN YDFTYVENVA+AH+CAE+ LAS G  AE+AAGQAYFITNMEPIKFWEF+S ILE
Sbjct: 213  GDGNNMYDFTYVENVAHAHICAEQALASEGKIAEQAAGQAYFITNMEPIKFWEFVSLILE 272

Query: 1082 GLGYERPRMKIPASVIMPIAHLVERTYKMLAQYGMRVPQLTPSRVRLLSCNRTFNCSKAK 903
            GLGYERPR+KIPA V+MPIAH+VE TYKMLA YGM+VPQLTPSR+RLLSC+RTF+CSKAK
Sbjct: 273  GLGYERPRIKIPALVMMPIAHMVEWTYKMLAPYGMKVPQLTPSRIRLLSCSRTFSCSKAK 332

Query: 902  SLLGYEPVVSLEDGLKRTVESYPQLRAEE--KTRMLSKASAYLGNGNIANALLWKDKKQX 729
              L Y P+VSL++GL+RT++SYP LRAE   K    SKAS YLG+G +A+ LLWKDKK  
Sbjct: 333  DQLSYTPIVSLQEGLRRTIDSYPDLRAEHLPKREGPSKASRYLGSGRVADTLLWKDKKLA 392

Query: 728  XXXXXXXXXXXXXXXTSGFTFIKATAKLLSVVALFLFIHGMLPEKIFSYKIEKISPTYFH 549
                            SG+T + A +KL  V ++FLFIHG LPEKI  Y +EKI P+ FH
Sbjct: 393  LTTLLVLMAFYHNFIASGYTIVTAVSKLFLVASIFLFIHGNLPEKILGYTVEKIPPSNFH 452

Query: 548  LSEEKSQCLAYSVTSSWNSAIGIVRSLCRGKDWPLFL 438
             SEE+S   A SV SSWN+A+ +++SLC+G DW LFL
Sbjct: 453  FSEERSHRAALSVASSWNTAVNVLKSLCKGDDWMLFL 489



 Score = 55.1 bits (131), Expect(2) = 0.0
 Identities = 27/63 (42%), Positives = 40/63 (63%)
 Frame = -1

Query: 358 KVFFMLLVLSFLASMSFQCIFKLAIPFLFIAFIVYEKWEDEIDGVVHNILSRAMKLKANV 179
           KV   LL+LSFL ++S Q +F   +   F+AF +YE  E+EID +    LS + KLK+++
Sbjct: 490 KVVLSLLILSFLGAISLQSLFVTGLLIAFVAFYIYENKEEEIDRLASEALSFSCKLKSDI 549

Query: 178 MKK 170
            KK
Sbjct: 550 AKK 552


>ref|NP_001050327.1| Os03g0405000 [Oryza sativa Japonica Group] gi|31415899|gb|AAP50920.1|
            putative 3-beta hydroxysteroid dehydrogenase/isomerase
            [Oryza sativa Japonica Group] gi|108708709|gb|ABF96504.1|
            NAD dependent epimerase/dehydratase family protein,
            expressed [Oryza sativa Japonica Group]
            gi|113548798|dbj|BAF12241.1| Os03g0405000 [Oryza sativa
            Japonica Group] gi|215678510|dbj|BAG92165.1| unnamed
            protein product [Oryza sativa Japonica Group]
          Length = 561

 Score =  649 bits (1674), Expect(2) = 0.0
 Identities = 310/454 (68%), Positives = 379/454 (83%)
 Frame = -3

Query: 1802 VKIMDLGPSIKLEPREEEGIIGQSLASGDAVYASADLRNKAQVIKAFEGAEVIFHMAAPD 1623
            V++ DL P+I L+  EEEGI+G +L  G A YASADLRNKAQV  AFEGA+V+FHMAAPD
Sbjct: 39   VRVADLPPAIALDRDEEEGILGAALREGRAAYASADLRNKAQVAAAFEGADVVFHMAAPD 98

Query: 1622 SSINNYQLHYSVNVEGMKNVIDACIECKVKRLICTSSPSVVFDGVHGLFNVDESMPYPEK 1443
            SSINN+ LHYSVNVEG KNVIDACI CKVKRLI TSSPSVVFDG+HG+F+ DESMPYP+K
Sbjct: 99   SSINNFHLHYSVNVEGTKNVIDACIRCKVKRLIYTSSPSVVFDGIHGIFDADESMPYPDK 158

Query: 1442 FNDAYSETKAEGEKLALKANGRNGLLTCCIRPSSIFGPGDRLLVPSLIAAAKAGKSKFII 1263
            FND+YSETKA+ EKL +KANGR+GLLTCCIRPSSIFGPGD+LLVPSL+ AA+AGKSK+II
Sbjct: 159  FNDSYSETKADAEKLVMKANGRDGLLTCCIRPSSIFGPGDKLLVPSLVTAARAGKSKYII 218

Query: 1262 GDGNNEYDFTYVENVAYAHVCAERTLASGGLSAEKAAGQAYFITNMEPIKFWEFMSRILE 1083
            GDG+N YDFTYVENVAY HVCAE+TL+S    A++AAG+ YFITNME IKFWEFMS ILE
Sbjct: 219  GDGSNYYDFTYVENVAYGHVCAEKTLSSED-GAKRAAGKTYFITNMEAIKFWEFMSLILE 277

Query: 1082 GLGYERPRMKIPASVIMPIAHLVERTYKMLAQYGMRVPQLTPSRVRLLSCNRTFNCSKAK 903
            GLGYERP +KIP SV+MP+AH+VE TYK  A+YGM++PQLTPSR+RLLSCNRTF+CS+AK
Sbjct: 278  GLGYERPSIKIPVSVMMPVAHMVEWTYKTFARYGMKIPQLTPSRIRLLSCNRTFSCSRAK 337

Query: 902  SLLGYEPVVSLEDGLKRTVESYPQLRAEEKTRMLSKASAYLGNGNIANALLWKDKKQXXX 723
              LGYEP+VSL+DGLKRT+ESYP L+A+ + R +SKAS +LGNGN+A  +LW+D KQ   
Sbjct: 338  DQLGYEPIVSLKDGLKRTIESYPHLQAQNQ-RSISKASIFLGNGNLAKTVLWEDMKQTMT 396

Query: 722  XXXXXXXXXXXXXTSGFTFIKATAKLLSVVALFLFIHGMLPEKIFSYKIEKISPTYFHLS 543
                         T G+TFI A AKL S+ ALFLFIHG+LP  +F +K+EK+ P+ FH++
Sbjct: 397  VLLLLAVIYYHLFTCGYTFITAMAKLFSLTALFLFIHGILPANVFGHKVEKLEPSNFHIT 456

Query: 542  EEKSQCLAYSVTSSWNSAIGIVRSLCRGKDWPLF 441
            + ++  +A+SV+S+WNS +G+++SLCRG DWPLF
Sbjct: 457  QVEAHHIAHSVSSTWNSLVGVLKSLCRGNDWPLF 490



 Score = 55.8 bits (133), Expect(2) = 0.0
 Identities = 28/51 (54%), Positives = 33/51 (64%)
 Frame = -1

Query: 358 KVFFMLLVLSFLASMSFQCIFKLAIPFLFIAFIVYEKWEDEIDGVVHNILS 206
           KV F LLV+S L+SMS Q  FK+ I   F+ F  YEKWED ID +V N  S
Sbjct: 492 KVVFSLLVVSILSSMSSQSAFKIGISMAFLGFKAYEKWEDTIDDLVGNACS 542


>emb|CBI20743.3| unnamed protein product [Vitis vinifera]
          Length = 540

 Score =  650 bits (1676), Expect(2) = 0.0
 Identities = 319/457 (69%), Positives = 376/457 (82%), Gaps = 2/457 (0%)
 Frame = -3

Query: 1802 VKIMDLGPSIKLEPREEEGIIGQSLASGDAVYASADLRNKAQVIKAFEGAEVIFHMAAPD 1623
            V+I DLGPSIKLEP EE+G++G++L SG A Y SADLR+KAQV+KA +GAE +FHMAAPD
Sbjct: 10   VRIADLGPSIKLEPSEEKGLLGEALQSGRAEYVSADLRDKAQVLKACQGAEAVFHMAAPD 69

Query: 1622 SSINNYQLHYSVNVEGMKNVIDACIECKVKRLICTSSPSVVFDGVHGLFNVDESMPYPEK 1443
            SSINN+QLH+SVNV+G KNVIDACIE KVKRLI TSSPSVVFDGVHG+FN DES+PYP+K
Sbjct: 70   SSINNHQLHHSVNVQGTKNVIDACIEQKVKRLIYTSSPSVVFDGVHGIFNGDESLPYPDK 129

Query: 1442 FNDAYSETKAEGEKLALKANGRNGLLTCCIRPSSIFGPGDRLLVPSLIAAAKAGKSKFII 1263
             ND+YS TKAEGE L +K+NG NGLLTCCIRPSSIFGPGD+LLVPSL+ AA+AGKSKFII
Sbjct: 130  HNDSYSATKAEGEALVIKSNGTNGLLTCCIRPSSIFGPGDKLLVPSLVNAARAGKSKFII 189

Query: 1262 GDGNNEYDFTYVENVAYAHVCAERTLASGGLSAEKAAGQAYFITNMEPIKFWEFMSRILE 1083
            GDGNN YDFTYVENVA+AH+CAE+ LAS G  AE+AAGQAYFITNMEPIKFWEF+S ILE
Sbjct: 190  GDGNNMYDFTYVENVAHAHICAEQALASEGKIAEQAAGQAYFITNMEPIKFWEFVSLILE 249

Query: 1082 GLGYERPRMKIPASVIMPIAHLVERTYKMLAQYGMRVPQLTPSRVRLLSCNRTFNCSKAK 903
            GLGYERPR+KIPA V+MPIAH+VE TYKMLA YGM+VPQLTPSR+RLLSC+RTF+CSKAK
Sbjct: 250  GLGYERPRIKIPALVMMPIAHMVEWTYKMLAPYGMKVPQLTPSRIRLLSCSRTFSCSKAK 309

Query: 902  SLLGYEPVVSLEDGLKRTVESYPQLRAEE--KTRMLSKASAYLGNGNIANALLWKDKKQX 729
              L Y P+VSL++GL+RT++SYP LRAE   K    SKAS YLG+G +A+ LLWKDKK  
Sbjct: 310  DQLSYTPIVSLQEGLRRTIDSYPDLRAEHLPKREGPSKASRYLGSGRVADTLLWKDKKLA 369

Query: 728  XXXXXXXXXXXXXXXTSGFTFIKATAKLLSVVALFLFIHGMLPEKIFSYKIEKISPTYFH 549
                            SG+T + A +KL  V ++FLFIHG LPEKI  Y +EKI P+ FH
Sbjct: 370  LTTLLVLMAFYHNFIASGYTIVTAVSKLFLVASIFLFIHGNLPEKILGYTVEKIPPSNFH 429

Query: 548  LSEEKSQCLAYSVTSSWNSAIGIVRSLCRGKDWPLFL 438
             SEE+S   A SV SSWN+A+ +++SLC+G DW LFL
Sbjct: 430  FSEERSHRAALSVASSWNTAVNVLKSLCKGDDWMLFL 466



 Score = 55.1 bits (131), Expect(2) = 0.0
 Identities = 27/63 (42%), Positives = 40/63 (63%)
 Frame = -1

Query: 358 KVFFMLLVLSFLASMSFQCIFKLAIPFLFIAFIVYEKWEDEIDGVVHNILSRAMKLKANV 179
           KV   LL+LSFL ++S Q +F   +   F+AF +YE  E+EID +    LS + KLK+++
Sbjct: 467 KVVLSLLILSFLGAISLQSLFVTGLLIAFVAFYIYENKEEEIDRLASEALSFSCKLKSDI 526

Query: 178 MKK 170
            KK
Sbjct: 527 AKK 529


>tpg|DAA45720.1| TPA: sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating [Zea
            mays]
          Length = 552

 Score =  640 bits (1652), Expect(2) = 0.0
 Identities = 310/456 (67%), Positives = 374/456 (82%)
 Frame = -3

Query: 1805 RVKIMDLGPSIKLEPREEEGIIGQSLASGDAVYASADLRNKAQVIKAFEGAEVIFHMAAP 1626
            RV++ DLGP+I L+  EEEG +G +L  G AVY SADLR+KAQV +AFEGAEV+FHMAAP
Sbjct: 35   RVRVADLGPAITLDRDEEEGFLGAALREGQAVYVSADLRDKAQVARAFEGAEVVFHMAAP 94

Query: 1625 DSSINNYQLHYSVNVEGMKNVIDACIECKVKRLICTSSPSVVFDGVHGLFNVDESMPYPE 1446
            DSSINN+ LHY VNV+G KNVID CI CKVKRLI TSSPSVVFDGVHG+FN DESMPYP+
Sbjct: 95   DSSINNFHLHYGVNVDGTKNVIDTCIRCKVKRLIYTSSPSVVFDGVHGIFNADESMPYPD 154

Query: 1445 KFNDAYSETKAEGEKLALKANGRNGLLTCCIRPSSIFGPGDRLLVPSLIAAAKAGKSKFI 1266
            KFND+YSETKA+ EKL ++ANGR GLLTCCIRPSSIFGPGD+L+VPSL+AAA+AGKSK+I
Sbjct: 155  KFNDSYSETKADAEKLVMRANGREGLLTCCIRPSSIFGPGDKLMVPSLVAAARAGKSKYI 214

Query: 1265 IGDGNNEYDFTYVENVAYAHVCAERTLASGGLSAEKAAGQAYFITNMEPIKFWEFMSRIL 1086
            IGDGNN YDFTYVENVAY HVCAE+TL+S    A+ AAG+ YFITNMEPIKFWEFMS IL
Sbjct: 215  IGDGNNYYDFTYVENVAYGHVCAEKTLSSED-GAKIAAGKTYFITNMEPIKFWEFMSLIL 273

Query: 1085 EGLGYERPRMKIPASVIMPIAHLVERTYKMLAQYGMRVPQLTPSRVRLLSCNRTFNCSKA 906
            EGLGYERP +KIP SV+MP+AH+VE TY+  A+YGM+VPQLTPSR+RLLSCNRTF+CS+A
Sbjct: 274  EGLGYERPSVKIPVSVMMPVAHVVEWTYQKFAKYGMKVPQLTPSRIRLLSCNRTFSCSRA 333

Query: 905  KSLLGYEPVVSLEDGLKRTVESYPQLRAEEKTRMLSKASAYLGNGNIANALLWKDKKQXX 726
            K  LGYEP+VSL+DG+KRTVESY  L+A+   R +SKAS +LGNGN+A  +LW+D KQ  
Sbjct: 334  KEQLGYEPLVSLKDGVKRTVESYSHLQAQNH-RSISKASIFLGNGNLAKTVLWEDAKQTV 392

Query: 725  XXXXXXXXXXXXXXTSGFTFIKATAKLLSVVALFLFIHGMLPEKIFSYKIEKISPTYFHL 546
                          T G+TFI A AKLLS+ ALFLFIHGMLP  +F +K+EK+  + FH+
Sbjct: 393  TVLLLLAVIYYHLFTCGYTFITAMAKLLSLTALFLFIHGMLPSNLFGHKVEKLEASNFHI 452

Query: 545  SEEKSQCLAYSVTSSWNSAIGIVRSLCRGKDWPLFL 438
            ++ ++  +A S++S+WNS +  +RSLCRG DW LFL
Sbjct: 453  TQAQAHHIANSISSNWNSLVSALRSLCRGNDWLLFL 488



 Score = 49.7 bits (117), Expect(2) = 0.0
 Identities = 24/46 (52%), Positives = 29/46 (63%)
 Frame = -1

Query: 358 KVFFMLLVLSFLASMSFQCIFKLAIPFLFIAFIVYEKWEDEIDGVV 221
           KV   LLV+S L+SMS Q  FK+    +F  F  YEKWED ID +V
Sbjct: 489 KVSLSLLVVSILSSMSSQAAFKIGTALVFTGFKAYEKWEDSIDSMV 534