BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dioscorea21_contig00006573
         (1322 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002285105.1| PREDICTED: isoflavone 2'-hydroxylase-like [V...   376   e-102
ref|XP_002320704.1| cytochrome P450 [Populus trichocarpa] gi|222...   370   e-100
ref|XP_002283222.2| PREDICTED: isoflavone 2'-hydroxylase-like [V...   370   e-100
ref|XP_002510130.1| cytochrome P450, putative [Ricinus communis]...   369   1e-99
gb|AGC29952.1| CYP81B57 [Sinopodophyllum hexandrum]                   366   6e-99

>ref|XP_002285105.1| PREDICTED: isoflavone 2'-hydroxylase-like [Vitis vinifera]
          Length = 499

 Score =  376 bits (966), Expect = e-102
 Identities = 194/423 (45%), Positives = 284/423 (67%), Gaps = 11/423 (2%)
 Frame = -2

Query: 1321 VVSSPSAAEHCFTKNDIIFANRPRILPAKYLGYNFTSLVFSPFTARWRAHRRFTALHAFS 1142
            +VSSPSA+E CFTKND++FANRPR++  K+LGYN+TS+ ++P    WR  RR ++    S
Sbjct: 75   LVSSPSASEECFTKNDVVFANRPRLIAGKHLGYNYTSMSWAPHGDHWRNLRRISSFEILS 134

Query: 1141 PS---CLSDNFSSELRFLLRGLYSDHTQGFHKTEIRSWFFDLTLNLMMGMITGRRFQGXX 971
             +    LS   S E+R L+R L+ + +Q     E++S FF++TLN+MM MI G+R+ G  
Sbjct: 135  SNRLQTLSGIRSDEVRSLVRWLFKNQSQ---MVEMKSAFFEMTLNVMMKMIGGKRYYGEN 191

Query: 970  XXXXXXXXXEM------FFLSGATSMEDFVPLVRWFGLSMMRKRLERVGKEIDETLQELV 809
                             F L+GAT+M DF+P++ W GL    +RL ++ K  +  +Q L+
Sbjct: 192  IGEVEEARMFREMVSETFQLAGATNMVDFLPILGWLGLKGTERRLIKLQKRRESFIQNLI 251

Query: 808  LEERSKLRNGDHGEEMKTIIGNLLTMQEKEPEHYTDIDIKGILASLIXXXXXXXXXTMEW 629
             E R K  N +  +  KT+I  LL++QE EPE+YTD  I+G++ S++         TMEW
Sbjct: 252  EEHRRKGSNCEGRQ--KTMIEVLLSLQETEPEYYTDEIIRGLMLSMLTGGTDTSAGTMEW 309

Query: 628  AMALLVNHPEVLKKVRDEIEEHVGHARLVMDSDITKLKYLNNVVKETLRLFPPGPLLVPH 449
            A++LL+N+P+VLKK   EI++ +GH RL+ + D+ +L YL +++KETLR++P GPLLVPH
Sbjct: 310  ALSLLLNNPKVLKKAHQEIDDRLGHDRLIEELDLAQLPYLRSIIKETLRMYPAGPLLVPH 369

Query: 448  ESSEDCIVEGVCVPKGTMLLVNAYAMQRDPELWEDSMKFKPERFD--EDHQQGFKYIPFG 275
            ESS++C V G  +P+GTMLLVN +A+Q D ++W D  +F+PERF+  E  + GFK++PFG
Sbjct: 370  ESSKECSVGGFRIPQGTMLLVNLWAIQSDHKIWGDPTEFRPERFEGVEGDRDGFKFVPFG 429

Query: 274  YGRRRCPGESMGWKVMTLALGVLIQCFEWEREGEELVDLTEGAGLSMPMATPLEIIYRPC 95
             GRR CPGE++  +++ LALG LIQCF+WER  E++VD+TEG GL++P A PL    RP 
Sbjct: 430  SGRRGCPGEALAIRIVGLALGSLIQCFDWERVDEQMVDMTEGGGLTLPKAQPLLAKCRPR 489

Query: 94   PEM 86
            P M
Sbjct: 490  PTM 492


>ref|XP_002320704.1| cytochrome P450 [Populus trichocarpa] gi|222861477|gb|EEE99019.1|
            cytochrome P450 [Populus trichocarpa]
          Length = 499

 Score =  370 bits (950), Expect = e-100
 Identities = 196/424 (46%), Positives = 274/424 (64%), Gaps = 12/424 (2%)
 Frame = -2

Query: 1321 VVSSPSAAEHCFTKNDIIFANRPRILPAKYLGYNFTSLVFSPFTARWRAHRRFTA---LH 1151
            VVSSPS AE CFTKND++FANRPR+L AK+L YN TSLV++P+   WR  RR  +   L 
Sbjct: 75   VVSSPSIAEECFTKNDVVFANRPRLLIAKHLAYNSTSLVWAPYGDHWRNLRRIVSIEVLS 134

Query: 1150 AFSPSCLSDNFSSELRFLLRGLYSDHTQGFHKTEIRSWFFDLTLNLMMGMITGRRFQGXX 971
            A+    LS     E++ ++  L+ +     H  ++++ FF+LTLN+MM MI G+R+ G  
Sbjct: 135  AYRLQMLSAIRLEEVKSMVCVLFRNQK---HTVDMKTVFFELTLNIMMRMIAGKRYYGEN 191

Query: 970  XXXXXXXXXEM------FFLSGATSMEDFVPLVRWFGLSMMRKRLERVGKEIDETLQELV 809
                             F L G T + D++P   W     M KRL     + D  LQ L+
Sbjct: 192  VSDVEEAKRFRALHAESFLLGGKTIIGDYIP---WIKSKKMEKRLIECNLKRDSFLQCLI 248

Query: 808  LEERSKLRNGDH-GEEMKTIIGNLLTMQEKEPEHYTDIDIKGILASLIXXXXXXXXXTME 632
             E+R K+  GD  GE+ K +I  LL++QE EPE+YTD  IKG++  ++         TME
Sbjct: 249  EEQRRKILEGDCCGEKKKNLIQVLLSLQETEPEYYTDDIIKGLVVVILFAGTDTSSTTME 308

Query: 631  WAMALLVNHPEVLKKVRDEIEEHVGHARLVMDSDITKLKYLNNVVKETLRLFPPGPLLVP 452
            WA++LL+NHPEVL+K + EI+EH+GH RL+ + D+ +L YL +++ ETLR++PP PLLVP
Sbjct: 309  WALSLLLNHPEVLEKAKREIDEHIGHDRLMDEGDLAQLPYLRSILNETLRMYPPAPLLVP 368

Query: 451  HESSEDCIVEGVCVPKGTMLLVNAYAMQRDPELWEDSMKFKPERFD--EDHQQGFKYIPF 278
            HESSE+C+V G  +P+GTML VN +A+Q DP++W D  KF+PERFD  E  +  FK +PF
Sbjct: 369  HESSEECLVGGFRIPRGTMLSVNMWAIQNDPKIWRDPTKFRPERFDNPEGGRYEFKLMPF 428

Query: 277  GYGRRRCPGESMGWKVMTLALGVLIQCFEWEREGEELVDLTEGAGLSMPMATPLEIIYRP 98
            G+GRR CPGE +  KV+ LALG L+QCFEW++ G+++VD+TE  G ++P A  LE I R 
Sbjct: 429  GHGRRSCPGEGLALKVVGLALGSLLQCFEWQKIGDKMVDMTESPGFTVPKAKQLEAICRA 488

Query: 97   CPEM 86
             P M
Sbjct: 489  RPRM 492


>ref|XP_002283222.2| PREDICTED: isoflavone 2'-hydroxylase-like [Vitis vinifera]
          Length = 503

 Score =  370 bits (949), Expect = e-100
 Identities = 202/424 (47%), Positives = 274/424 (64%), Gaps = 12/424 (2%)
 Frame = -2

Query: 1321 VVSSPSAAEHCFTKNDIIFANRPRILPAKYLGYNFTSLVFSPFTARWRAHRRFTALHAFS 1142
            +VSSPSAAE CFTKNDI+FANRPR+L  K+LGYN+T+L ++P+   WR  RR  +L   S
Sbjct: 75   IVSSPSAAEECFTKNDIVFANRPRLLAGKHLGYNYTTLTWAPYGQHWRNLRRIASLEILS 134

Query: 1141 PSCLS---DNFSSELRFLLRGLYSDHTQG-FHKTEIRSWFFDLTLNLMMGMITGRRFQGX 974
             + L    D    E+R LL  L+   ++G F   +++S FF+LTLN MM MI+G+R+ G 
Sbjct: 135  SNRLQMFYDIRIDEVRALLCQLFRASSEGQFSAVDMKSMFFELTLNNMMRMISGKRYYGD 194

Query: 973  XXXXXXXXXXEM------FFLSGATSMEDFVPLVRWFGLSMMRKRLERVGKEIDETLQEL 812
                              F LSGAT++ DFVP  +W GL+ + K+L  +  + D  +Q L
Sbjct: 195  NVTELEETRKFREIVAETFELSGATNIVDFVPFSKWIGLNGIEKKLVILQGKRDGFMQNL 254

Query: 811  VLEERSKLRNGDHGEEMKTIIGNLLTMQEKEPEHYTDIDIKGILASLIXXXXXXXXXTME 632
            + EE  ++R+   G   KT++  LL++QE EPE YTD  I+G++  ++         TME
Sbjct: 255  I-EEHRRMRSPCEGRS-KTMLDVLLSLQETEPECYTDDIIRGMMQVMLSAGTDTSAGTME 312

Query: 631  WAMALLVNHPEVLKKVRDEIEEHVGHARLVMDSDITKLKYLNNVVKETLRLFPPGPLLVP 452
            WAM+LL+N+PE L+K + EI+ H+G +RL+ + DI +L YL  ++KETLR++P  PLLVP
Sbjct: 313  WAMSLLLNNPEALEKAQAEIDSHLGKSRLIDELDIAELPYLRGIIKETLRMYPAAPLLVP 372

Query: 451  HESSEDCIVEGVCVPKGTMLLVNAYAMQRDPELWEDSMKFKPERFD--EDHQQGFKYIPF 278
            HESSE+C V G  VP GTMLLVN +A+Q DP LW +  KFKPERF   E  + GF + PF
Sbjct: 373  HESSEECTVGGFRVPSGTMLLVNMWAIQNDPMLWAEPSKFKPERFQGPEGQRNGFMFSPF 432

Query: 277  GYGRRRCPGESMGWKVMTLALGVLIQCFEWEREGEELVDLTEGAGLSMPMATPLEIIYRP 98
            G GRR CPGE +  +V+ LALG LIQ FEWER  EE+VD++EG GL+MP A  L    RP
Sbjct: 433  GAGRRGCPGEGLAMRVVGLALGSLIQFFEWERVDEEMVDMSEGTGLTMPKAQSLVAKCRP 492

Query: 97   CPEM 86
             P M
Sbjct: 493  RPSM 496


>ref|XP_002510130.1| cytochrome P450, putative [Ricinus communis]
            gi|223550831|gb|EEF52317.1| cytochrome P450, putative
            [Ricinus communis]
          Length = 500

 Score =  369 bits (946), Expect = 1e-99
 Identities = 191/423 (45%), Positives = 270/423 (63%), Gaps = 11/423 (2%)
 Frame = -2

Query: 1321 VVSSPSAAEHCFTKNDIIFANRPRILPAKYLGYNFTSLVFSPFTARWRAHRRFTALHAFS 1142
            VVSSPSAAE C TK D +FANRPR+L  K++GYN+TSL ++P+   WR  R+ +AL   S
Sbjct: 73   VVSSPSAAEECLTKCDTVFANRPRLLAGKHIGYNYTSLAWAPYGDLWRNLRKVSALEILS 132

Query: 1141 P---SCLSDNFSSELRFLLRGLYSDHTQGFHKTEIRSWFFDLTLNLMMGMITGRRFQGXX 971
                  LS     E++ L++ L+ ++     K +++S FF+L LN+MM MI G+R+ G  
Sbjct: 133  SHRLQLLSSIRRDEVKLLIQRLFRNNKDCREKVDMKSAFFELMLNVMMRMIAGKRYYGEN 192

Query: 970  XXXXXXXXXEM------FFLSGATSMEDFVPLVRWFGLSMMRKRLERVGKEIDETLQELV 809
                             F  +G ++M DF+PLV   G     KRL+ +GK  D  +Q+L+
Sbjct: 193  VEQVEEAKRFREIVRETFLAAGTSNMGDFLPLVAVVG--GQEKRLKELGKRRDGFIQDLI 250

Query: 808  LEERSKLRNGDHGEEMKTIIGNLLTMQEKEPEHYTDIDIKGILASLIXXXXXXXXXTMEW 629
             E R ++      E  KT+I  LL++QE EPE+YTD  IK ++  L+         TMEW
Sbjct: 251  DEHRKRMAACSSEERNKTMIEVLLSLQESEPEYYTDETIKSLMLVLLAAGTDTSAATMEW 310

Query: 628  AMALLVNHPEVLKKVRDEIEEHVGHARLVMDSDITKLKYLNNVVKETLRLFPPGPLLVPH 449
            AM+LLVN+PE LKK + EI+  +GH RL+ +SD +KL YLN ++ E +R++P GPLLVPH
Sbjct: 311  AMSLLVNNPEALKKAQTEIDSVIGHDRLINESDTSKLPYLNCIINEVMRMYPAGPLLVPH 370

Query: 448  ESSEDCIVEGVCVPKGTMLLVNAYAMQRDPELWEDSMKFKPERFD--EDHQQGFKYIPFG 275
            ESSE+C + G  VP GTMLLVN +++Q DP +WE+   FKPERF+  E  + GF+ +PFG
Sbjct: 371  ESSEECFIGGYRVPAGTMLLVNLWSIQNDPRVWEEPRNFKPERFEGCEGVRDGFRLMPFG 430

Query: 274  YGRRRCPGESMGWKVMTLALGVLIQCFEWEREGEELVDLTEGAGLSMPMATPLEIIYRPC 95
             GRR CPGE +  +++ L +G L+QCF+WER G+E++D+TEG GL+MP A PL +   P 
Sbjct: 431  SGRRSCPGEGLALRMVGLGIGTLLQCFDWERVGKEMIDMTEGVGLTMPKAQPLVVQCSPR 490

Query: 94   PEM 86
            P M
Sbjct: 491  PSM 493


>gb|AGC29952.1| CYP81B57 [Sinopodophyllum hexandrum]
          Length = 507

 Score =  366 bits (940), Expect = 6e-99
 Identities = 196/424 (46%), Positives = 278/424 (65%), Gaps = 16/424 (3%)
 Frame = -2

Query: 1321 VVSSPSAAEHCFTKNDIIFANRPRILPAKYLGYNFTSLVFSPFTARWRAHRRFTALHAFS 1142
            VVSSPSAAE C TKNDI FANRPR++  K+ G N+T+L ++ +   WR  RR +AL  FS
Sbjct: 76   VVSSPSAAEECLTKNDIGFANRPRLMIGKHSGNNYTTLAWASYGQNWRNLRRISALEMFS 135

Query: 1141 PSCL---SDNFSSELRFLLRGLYSDHTQGFHKTEIRSWFFDLTLNLMMGMITGRRFQGXX 971
            P+ +   S+  + E++ ++R L + +   +HK E++S FF+LTLN+MM MI G+R+ G  
Sbjct: 136  PNRIQMFSEIRTDEVKSMVRRLVAGYE--YHKVEMKSVFFELTLNIMMRMIAGKRYYGEH 193

Query: 970  XXXXXXXXXEM------FFLSGATSMEDFVPLVRWFGLSMMRKRLERVGKEIDETLQELV 809
                             F LSGA+++ DF+PL+RW G   + KRL R+  + DE LQEL+
Sbjct: 194  VADLEQARKFKEIVEETFELSGASNVGDFLPLLRWIGYKGIEKRLIRLKNKRDEFLQELI 253

Query: 808  LEERSKLRNGDHG----EEMKTIIGNLLTMQEKEPEHYTDIDIKGILASLIXXXXXXXXX 641
             +ER KLR         E+ KT+I  LL++QE EPE+YTD  I+G++  L          
Sbjct: 254  -QERRKLRVNSKSFPSEEKKKTLIDVLLSLQETEPEYYTDEMIRGLIWILFAAGTDTSAG 312

Query: 640  TMEWAMALLVNHPEVLKKVRDEIEEHVGHARLVMDSDITKLKYLNNVVKETLRLFPPGPL 461
            TMEWAM+LL+N+P+++KK + EI+  +   R + +SD+ KL YL+ ++ ETLR++P GPL
Sbjct: 313  TMEWAMSLLLNNPDLIKKAQSEIDLQIEPGRPIDESDLNKLPYLHCIINETLRMYPAGPL 372

Query: 460  LVPHESSEDCIVEGVCVPKGTMLLVNAYAMQRDPELWEDSMKFKPERFDEDH---QQGFK 290
            L+PHESSEDC + G  +P GTMLLVN +A+Q DP LW++  KFKPERF+      + GFK
Sbjct: 373  LIPHESSEDCEIGGYNIPCGTMLLVNLWAIQNDPNLWKEPRKFKPERFEGYQGGVRDGFK 432

Query: 289  YIPFGYGRRRCPGESMGWKVMTLALGVLIQCFEWEREGEELVDLTEGAGLSMPMATPLEI 110
             +PFG GRR CPGE +  +V+ L L  L+QCF+W R GEE+V+++EG GL++P   PLE 
Sbjct: 433  LMPFGSGRRGCPGEGLAXRVVGLTLXSLLQCFDWHRVGEEMVEMSEGTGLTLPKLHPLEA 492

Query: 109  IYRP 98
              RP
Sbjct: 493  HCRP 496