BLASTX nr result
ID: Dioscorea21_contig00005871
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dioscorea21_contig00005871
(1497 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_003563042.1| PREDICTED: probable 5'-adenylylsulfate reduc... 96 1e-55
dbj|BAK04337.1| predicted protein [Hordeum vulgare subsp. vulgare] 95 6e-53
gb|AAB57688.1| APS reductase [Arabidopsis thaliana] gi|21617937|... 89 1e-52
ref|XP_002886487.1| hypothetical protein ARALYDRAFT_475126 [Arab... 89 1e-52
gb|AAB05871.2| PAPS-reductase-like protein [Catharanthus roseus] 94 1e-51
>ref|XP_003563042.1| PREDICTED: probable 5'-adenylylsulfate reductase 1,
chloroplastic-like [Brachypodium distachyon]
Length = 463
Score = 95.5 bits (236), Expect(5) = 1e-55
Identities = 43/58 (74%), Positives = 50/58 (86%)
Frame = -2
Query: 1121 QKHYDMHIEYMVPDAREVQKLVRSKGLFSFYGDGHQKYCRVREVRPFGRALKGLNAWI 948
+KHY +HIEYM P+A EVQ+LVR+KGLFSFY DGHQ+ CRVR+VRP RALKGL AWI
Sbjct: 159 EKHYGIHIEYMFPEAAEVQELVRNKGLFSFYEDGHQECCRVRKVRPLRRALKGLKAWI 216
Score = 67.8 bits (164), Expect(5) = 1e-55
Identities = 33/41 (80%), Positives = 35/41 (85%)
Frame = -1
Query: 1350 SHDGLFLSGAEDVALIEYARLTGWPFRVFSLDTGRLNPETY 1228
S + SGAEDVALIEYA+LTG PFRVFSLDTGRLNPETY
Sbjct: 112 SEIAIAFSGAEDVALIEYAKLTGRPFRVFSLDTGRLNPETY 152
Score = 65.5 bits (158), Expect(5) = 1e-55
Identities = 41/117 (35%), Positives = 56/117 (47%), Gaps = 4/117 (3%)
Frame = -2
Query: 668 GYVCIGCEPYSLPALPGQHXXXXXXXXXXXXXXXXGMQSP*R*YQARRHT*KIWYQCEWN 489
GYV IGCEP + P LPGQH G+ + K+ +
Sbjct: 282 GYVSIGCEPCTRPVLPGQHEREGRWWWEDATAKECGLHKGN--IEKEGQAPKVGVNGNGS 339
Query: 488 CQSRR----LSESTHSLSRPGIENLLNLQNRIEL*LVVLYSPWC*FCQIKTLRFLRI 330
++ S++ SL+RPGIENLL L+NR E L VLY+PWC +CQ +L +
Sbjct: 340 AEASAPDIFQSQAVVSLTRPGIENLLKLENRTEPWLTVLYAPWCPYCQAMEASYLEL 396
Score = 51.6 bits (122), Expect(5) = 1e-55
Identities = 22/34 (64%), Positives = 28/34 (82%)
Frame = -1
Query: 855 SLIKWNPLLNIQGKDMWNFLRTMNVPCKISLHPQ 754
SLIKWNP+ N++GKD+W FLRTM+VP +LH Q
Sbjct: 249 SLIKWNPVANVEGKDIWTFLRTMDVPVN-TLHAQ 281
Score = 26.6 bits (57), Expect(5) = 1e-55
Identities = 12/24 (50%), Positives = 17/24 (70%)
Frame = -1
Query: 228 IKVAKFQADGEQRKVKRLKATGQS 157
+KVAKF+ADGEQ+ + + QS
Sbjct: 405 VKVAKFRADGEQKPFAQAELQLQS 428
>dbj|BAK04337.1| predicted protein [Hordeum vulgare subsp. vulgare]
Length = 458
Score = 94.7 bits (234), Expect(4) = 6e-53
Identities = 43/58 (74%), Positives = 49/58 (84%)
Frame = -2
Query: 1121 QKHYDMHIEYMVPDAREVQKLVRSKGLFSFYGDGHQKYCRVREVRPFGRALKGLNAWI 948
+KHY +HIEYM P+A EVQ LVR+KGLFSFY DGHQ+ CRVR+VRP RALKGL AWI
Sbjct: 154 EKHYGIHIEYMFPEASEVQDLVRTKGLFSFYEDGHQECCRVRKVRPLRRALKGLKAWI 211
Score = 67.8 bits (164), Expect(4) = 6e-53
Identities = 33/41 (80%), Positives = 35/41 (85%)
Frame = -1
Query: 1350 SHDGLFLSGAEDVALIEYARLTGWPFRVFSLDTGRLNPETY 1228
S + SGAEDVALIEYA+LTG PFRVFSLDTGRLNPETY
Sbjct: 107 SEIAIAFSGAEDVALIEYAKLTGRPFRVFSLDTGRLNPETY 147
Score = 63.9 bits (154), Expect(4) = 6e-53
Identities = 40/117 (34%), Positives = 56/117 (47%), Gaps = 4/117 (3%)
Frame = -2
Query: 668 GYVCIGCEPYSLPALPGQHXXXXXXXXXXXXXXXXGMQSP*R*YQARRHT*KIWYQCEWN 489
GYV IGCEP + P LPGQH G+ T K+ +
Sbjct: 277 GYVSIGCEPCTRPVLPGQHEREGRWWWEDATAKECGLHKGN--IDKEGQTPKVGVNGNGS 334
Query: 488 CQSRR----LSESTHSLSRPGIENLLNLQNRIEL*LVVLYSPWC*FCQIKTLRFLRI 330
++ S++ +L+RPGIENLL L+NR E L VLY+PWC +CQ ++ +
Sbjct: 335 AEASAPDIFQSQAIVNLTRPGIENLLRLENRAEPWLAVLYAPWCPYCQAMEASYVEL 391
Score = 50.8 bits (120), Expect(4) = 6e-53
Identities = 22/34 (64%), Positives = 27/34 (79%)
Frame = -1
Query: 855 SLIKWNPLLNIQGKDMWNFLRTMNVPCKISLHPQ 754
SLIKWNP+ N+ GKD+W FLRTM+VP +LH Q
Sbjct: 244 SLIKWNPVANVDGKDIWTFLRTMDVPVN-TLHAQ 276
>gb|AAB57688.1| APS reductase [Arabidopsis thaliana] gi|21617937|gb|AAM66987.1|
5'-adenylylphosphosulfate reductase, putative
[Arabidopsis thaliana]
Length = 455
Score = 89.4 bits (220), Expect(5) = 1e-52
Identities = 42/58 (72%), Positives = 47/58 (81%)
Frame = -2
Query: 1121 QKHYDMHIEYMVPDAREVQKLVRSKGLFSFYGDGHQKYCRVREVRPFGRALKGLNAWI 948
+K Y + IEYM PDA EVQ LVR+KGLFSFY DGHQ+ CRVR+VRP RALKGL AWI
Sbjct: 158 EKQYGIRIEYMFPDAVEVQALVRNKGLFSFYEDGHQECCRVRKVRPLRRALKGLKAWI 215
Score = 68.6 bits (166), Expect(5) = 1e-52
Identities = 33/34 (97%), Positives = 33/34 (97%)
Frame = -1
Query: 1329 SGAEDVALIEYARLTGWPFRVFSLDTGRLNPETY 1228
SGAEDVALIEYARLTG PFRVFSLDTGRLNPETY
Sbjct: 118 SGAEDVALIEYARLTGKPFRVFSLDTGRLNPETY 151
Score = 59.7 bits (143), Expect(5) = 1e-52
Identities = 41/115 (35%), Positives = 54/115 (46%), Gaps = 2/115 (1%)
Frame = -2
Query: 668 GYVCIGCEPYSLPALPGQHXXXXXXXXXXXXXXXXGMQSP*R*YQARRHT*KIWYQCEWN 489
GYV IGCEP + P LPGQH G+ + K
Sbjct: 281 GYVSIGCEPCTRPVLPGQHEREGRWWWEDAKAKECGLHKGNIKEEDGAADSK-------P 333
Query: 488 CQSRRLSESTH--SLSRPGIENLLNLQNRIEL*LVVLYSPWC*FCQIKTLRFLRI 330
+ + ES + +LSR G+ENLL L+NR E LVVLY+PWC FCQ ++ +
Sbjct: 334 AAVQEIFESNNVVALSRGGVENLLKLENRKEAWLVVLYAPWCPFCQAMEASYVEL 388
Score = 52.4 bits (124), Expect(5) = 1e-52
Identities = 22/34 (64%), Positives = 28/34 (82%)
Frame = -1
Query: 855 SLIKWNPLLNIQGKDMWNFLRTMNVPCKISLHPQ 754
SL+KWNPL N++G D+WNFLRTM+VP +LH Q
Sbjct: 248 SLVKWNPLANVEGADVWNFLRTMDVPVN-ALHAQ 280
Score = 26.6 bits (57), Expect(5) = 1e-52
Identities = 10/14 (71%), Positives = 14/14 (100%)
Frame = -1
Query: 228 IKVAKFQADGEQRK 187
+KVAKF+ADGEQ++
Sbjct: 397 VKVAKFRADGEQKE 410
>ref|XP_002886487.1| hypothetical protein ARALYDRAFT_475126 [Arabidopsis lyrata subsp.
lyrata] gi|297332328|gb|EFH62746.1| hypothetical protein
ARALYDRAFT_475126 [Arabidopsis lyrata subsp. lyrata]
Length = 455
Score = 89.4 bits (220), Expect(5) = 1e-52
Identities = 42/58 (72%), Positives = 47/58 (81%)
Frame = -2
Query: 1121 QKHYDMHIEYMVPDAREVQKLVRSKGLFSFYGDGHQKYCRVREVRPFGRALKGLNAWI 948
+K Y + IEYM PDA EVQ LVR+KGLFSFY DGHQ+ CRVR+VRP RALKGL AWI
Sbjct: 158 EKQYGIRIEYMFPDAVEVQALVRNKGLFSFYEDGHQECCRVRKVRPLRRALKGLKAWI 215
Score = 68.6 bits (166), Expect(5) = 1e-52
Identities = 33/34 (97%), Positives = 33/34 (97%)
Frame = -1
Query: 1329 SGAEDVALIEYARLTGWPFRVFSLDTGRLNPETY 1228
SGAEDVALIEYARLTG PFRVFSLDTGRLNPETY
Sbjct: 118 SGAEDVALIEYARLTGKPFRVFSLDTGRLNPETY 151
Score = 59.3 bits (142), Expect(5) = 1e-52
Identities = 40/115 (34%), Positives = 54/115 (46%), Gaps = 2/115 (1%)
Frame = -2
Query: 668 GYVCIGCEPYSLPALPGQHXXXXXXXXXXXXXXXXGMQSP*R*YQARRHT*KIWYQCEWN 489
GYV IGCEP + P LPGQH G+ + K
Sbjct: 281 GYVSIGCEPCTRPVLPGQHEREGRWWWEDAKAKECGLHKGNIKEEDGAADSK-------P 333
Query: 488 CQSRRLSESTH--SLSRPGIENLLNLQNRIEL*LVVLYSPWC*FCQIKTLRFLRI 330
+ + ES + +LSR G+ENL+ L+NR E LVVLY+PWC FCQ ++ +
Sbjct: 334 AAVQEIFESNNVVALSRGGVENLMKLENRKEAWLVVLYAPWCPFCQAMEASYIEL 388
Score = 52.8 bits (125), Expect(5) = 1e-52
Identities = 22/34 (64%), Positives = 28/34 (82%)
Frame = -1
Query: 855 SLIKWNPLLNIQGKDMWNFLRTMNVPCKISLHPQ 754
SL+KWNPL N++G D+WNFLRTM+VP +LH Q
Sbjct: 248 SLVKWNPLANVEGSDVWNFLRTMDVPVN-ALHAQ 280
Score = 26.6 bits (57), Expect(5) = 1e-52
Identities = 10/14 (71%), Positives = 14/14 (100%)
Frame = -1
Query: 228 IKVAKFQADGEQRK 187
+KVAKF+ADGEQ++
Sbjct: 397 VKVAKFRADGEQKE 410
>gb|AAB05871.2| PAPS-reductase-like protein [Catharanthus roseus]
Length = 464
Score = 93.6 bits (231), Expect(4) = 1e-51
Identities = 44/58 (75%), Positives = 48/58 (82%)
Frame = -2
Query: 1121 QKHYDMHIEYMVPDAREVQKLVRSKGLFSFYGDGHQKYCRVREVRPFGRALKGLNAWI 948
+K Y +HIEYM PDA EVQ LVRSKGLFSFY DGHQ+ CRVR+VRP RALKGL AWI
Sbjct: 160 EKQYGIHIEYMFPDAVEVQALVRSKGLFSFYEDGHQECCRVRKVRPLRRALKGLRAWI 217
Score = 66.6 bits (161), Expect(4) = 1e-51
Identities = 32/34 (94%), Positives = 32/34 (94%)
Frame = -1
Query: 1329 SGAEDVALIEYARLTGWPFRVFSLDTGRLNPETY 1228
SGAEDVALIEYA LTG PFRVFSLDTGRLNPETY
Sbjct: 120 SGAEDVALIEYAHLTGRPFRVFSLDTGRLNPETY 153
Score = 61.2 bits (147), Expect(4) = 1e-51
Identities = 41/115 (35%), Positives = 54/115 (46%), Gaps = 2/115 (1%)
Frame = -2
Query: 668 GYVCIGCEPYSLPALPGQHXXXXXXXXXXXXXXXXGMQSP*R*YQARRHT*KIWYQCEWN 489
GYV IGCEP + P LPGQH G+ + + +
Sbjct: 283 GYVSIGCEPCTRPVLPGQHEREGRWWWEDAKAKECGLHKGNIKEETLNNNGNGAVNGNGS 342
Query: 488 CQSRRLSESTH--SLSRPGIENLLNLQNRIEL*LVVLYSPWC*FCQIKTLRFLRI 330
+ ++ + SLSRPGIENLL L+ R E LVVLY+PWC FCQ +L +
Sbjct: 343 DTIADIFDTNNVTSLSRPGIENLLKLEERREAWLVVLYAPWCRFCQAMEGSYLEL 397
Score = 51.6 bits (122), Expect(4) = 1e-51
Identities = 21/34 (61%), Positives = 28/34 (82%)
Frame = -1
Query: 855 SLIKWNPLLNIQGKDMWNFLRTMNVPCKISLHPQ 754
SL+KWNP+ N++GKD+WNFLR M+VP +LH Q
Sbjct: 250 SLVKWNPVANVEGKDIWNFLRAMDVPVN-TLHSQ 282